KR20150111786A - Manufacturing method for Sunsik using Jeungpo (steamed and dried) method and Sunsik prepared by the method - Google Patents

Abstract

The present invention provides a method for producing a functional grain powder meal from grains and herbal medicinal materials having effects in preventing and alleviating insulin resistance syndrome by using a steaming and drying method. Moreover, the present invention also provides a functional grain powder meal produced through the method and exhibiting effects in alleviating and preventing insulin resistance syndrome. The functional grain powder produced according to the present invention exhibits effects in alleviating symptoms of insulin resistance syndrome such as obesity, blood sugar, insulin resistance, and hyperlipemia. Functional properties such as taste, color, fragrance, and texture, nutritional components, and physiological activities are also more excellent than or exhibit at least a similar level to a functional grain powder meal currently purchased in the market, so the functional grain powder meal of the present invention can be widely used as a meal substitution food or a health supplementary food in all ages.

Description

Technical Field [0001] The present invention relates to a method of manufacturing an electric wire using a graining method,

The present invention relates to a method of manufacturing an electric wire using a vaporizing method and a functional wire made therefrom. The functional wire of the present invention includes selected organic cereals and herbal ingredients, Or preventive effect.

Guizhou Guo Pu is a method used to make medicines for a long time, which means that it is necessary to sift and dry the medicines nine times. Some herbal medicines need to be processed before the preparation of medicines because it is necessary to strengthen or soften the action of the medicines, to soften the hard ones, or to remove the toxicities. Among them, the medicines are put in a syringe and the kettle is filled with water After the steam is placed on the pot, it is called "steam" and the medicine is dried in the sun.

On the other hand, Sunshik (禪 食) is known as one of the convenient and nutritious natural health remedies to carry from the Silla era, and developed into a food that can be eaten as a substitute for meals to modern people who are busy daily life. In addition to the conventional seven-grain mixture of brown rice, glutinous rice, barley, black bean, black sesame, perilla, and yulmu, we also sell functional foods such as dried vegetables, mushrooms, bulbs, seafood, fruits, This is widely sold. Examples of functional alopecia include, for example, Korean Patent No. 10-0615987, which enhances the nutritional balance effect in terms of nutrition, and emphasizes the effects of liver and gastrointestinal function, Open No. 10-2005-0047053 entitled " Food substitute food on the theme of brown rice and various nutritional materials ", Korean Patent No. 10-0664480 "Functional wire for high protein diets " have.

However, functional algebra is not yet known, which has been scientifically proven to prevent or ameliorate metabolic syndrome such as obesity, blood sugar and hypertension, which are problematic for modern people due to the high fat diet. Accordingly, the present inventors have selected the food materials capable of improving the metabolic syndrome and confirmed the efficacy of the functional sunscreen prepared by applying the corneal topography method, and completed the present invention.

It is an object of the present invention to provide a method for producing a functional wire from materials such as cereals and herbal medicines which have an effect of preventing and improving metabolic syndrome by using a vapor method. Another object of the present invention is to provide a functional wire which exhibits the improvement and prevention effects of the metabolic syndrome and the like produced from the above-mentioned production method.

In order to achieve the above-mentioned object, the present invention relates to a method for producing a fermented soybean curd, comprising the steps of: Stevia, burdock, shiitake, cabbage, lotus root and spinach.

The manufacturing method of the present invention comprises the steps of: (a) cleaning materials selected from the above 23 kinds of food materials; (b) growing the materials; And (c) cooling the pre-heated materials.

In one embodiment of the present invention, the materials of step (a) may have the composition ratios shown in Table 1 below.

In one embodiment of the present invention, the step (a) may be performed at room temperature for 14 hours, but is not limited thereto.

In one embodiment, the step (b) may be performed using a hot pot. In this case, when using a large-capacity hot pot, the temperature is raised to 115 ° C for 2 hours 30 minutes. When the small- But is not limited thereto.

In one embodiment of the present invention, the step (c) may be performed by discharging heat at a room temperature until the internal temperature reaches 75 to 85 ° C. In one embodiment of the present invention, the step (c) may be performed for 10 minutes.

In one embodiment of the present invention, the steps (b) and (c) may be repeated two, six or eight more times, and in particular, eight more times, ) Law.

In one embodiment of the present invention, the method comprises: (d) drying the cooled materials; (e) roasting the dried materials; And (f) milling the roasted ingredients.

In one embodiment of the present invention, the moisture content of the materials dried in step (d) is preferably not more than 10% by weight, and in this case, step (d) Lt; 0 > C for 40 hours.

In one embodiment of the present invention, the step (e) may be performed at 90 to 95 ° C for 6 to 7 minutes using a frying pan, but is not limited thereto.

In one embodiment of the present invention, step (f) may be carried out using milling, but is not limited thereto. Preferably, milling is performed at a size of about 100 to 120 mesh.

In addition, the present invention provides a functional wire made according to the above production method.

In one embodiment of the present invention, the functional line shows the effect of preventing or symptomatic improvement of the metabolic syndrome.

The functional line manufactured according to the present invention has the effect of improving the symptoms of metabolic syndrome such as obesity, blood sugar, insulin resistance and hyperlipidemia, but also has the sensory characteristics such as taste, color, flavor and texture, It is superior or at least comparable to the functional seaweed being sold, so it can be widely used as a dietary substitute or a health supplement in all ages.

FIGS. 1A and 1B illustrate a method of manufacturing a functional wire using a cored wire method according to an embodiment of the present invention.
FIG. 2 shows experimental results showing the effect of the present invention on blood glucose concentration.
FIG. 3 is a graph showing the effect of the present invention on (a) insulin concentration, (b) insulin resistance, and (c) insulin secretion ability.
FIG. 4 is a graph showing the effect of the present invention on the activity of (a) adiponectin and (b) leptin.
FIG. 5 shows experimental results showing the effect of the present line of action on (a) GOT and (b) GPT activity.
FIG. 6 shows the results of experiments showing the effect of the present invention on (a) total bilirubin, (b) direct bilirubin, and (c) indirect bilirubin.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

< Example  1>

Functionality according to the present invention Solar  Produce

Various foods belonging to bean curd, cereals, vegetables, fruits and the like which are expected to be related to the prevention and improvement of metabolic syndrome were tested by different combinations and composition ratios, Were selected.

The functional wire of the present invention was prepared according to the following production method using the above-mentioned ingredients, which is known as a Korean traditional herbal medicine manufacturing method (Fig. 1):

(1) Wheat, bean, white crustacea, burdock, red bean, black rice, brown rice, glutinous rice barley brown rice, sorghum, corn, chrysanthemum, apple, strawberry, pumpkin, potato, citrus, yulmu, stevia, burdock, , Lotus root and spinach and then called for 14 hours at room temperature;

(2) heating the above materials for 2 hours 30 minutes or more at a temperature of 115 ° C or higher or 105 ° C for 50 minutes using a small-capacity hot-grinder by using a large-capacity electric hot pot,

(3) a cooling step in which the heated materials are allowed to stand at room temperature for about 10 minutes to discharge hot heat;

(4) optionally repeating steps (2) and (3) two, six or eight more times;

(5) drying the cooled materials at 60 DEG C for 40 hours using an electric drier to make the water content to 10% by weight or less;

(6) roasting the dried materials at 90 to 95 DEG C for 6 to 7 minutes according to physical properties using a stirrer;

(7) pulverizing the roasted materials to a size of about 100 to 120 meshes using a mill; And

(8) blending the pulverized materials in the composition ratio shown in Table 1 to make a wire powder.

The functional wire according to the present embodiment was manufactured into four kinds of products according to the step (4). That is, for comparative experiments, the products of 1-fold increase in volume and 1-fold increase in cooling, 3-fold increase in 3-fold increase and decrease, 7-fold decrease in 7-fold increase in total, 9 product.

< Example  2>

The functionality of the present invention On the line  Sensory evaluation

For the four kinds of products manufactured according to the above Example 1, the food and nutrition related persons were selected as evaluation personnel and rated 5 points according to the items such as taste, color, flavor, texture and overall flavor. The evaluation score for each characteristic was evaluated as "very bad" as 1 point and "very good" as 5 points.

The results of the sensory test on the commercially available fermented wire, 12 curves and the above four types of wire are shown in Table 2 below.

As a result of the sensory evaluation, the taste of 7 - cell 7 - cell and 9 - cell 9 cell were better than those of fermentation and 12 - line type, and there was no significant difference in flavor compared to fermentation and 12 - . There was no significant difference in texture and color between fermentation and twelve lines. The overall taste was similar to that of fermentation and twelve lines of 7-for-7 and 9-for-9 varieties. Therefore, the overall sensory test results are considered to be similar to those on the market of 7-cell 7-cell and 9-cell 9-cell.

< Example  3>

The functionality of the present invention Solar  Analysis of Nutritional and Functional Components

<3-1> Nutritional analysis

Nutritional evaluation of nutrient content per 100g of the prepared seoyun was calculated using CANPro 3.0 (Computer Aided Nutritional Analysis program) developed by the Dietitian Society of Japan. Based on these results, we analyzed the contribution of age and gender to nutrient intake standards in comparison with Korean nutrition standard.

Table 3 presents the mineral, antioxidant and water-soluble vitamin contents per 100 g of the line product. Among inorganic minerals, calcium was 99.9 mg, phosphorus was 319.4 mg, iron was 3.88 mg, sodium was 4.56 mg, and potassium was 705.6 mg. Among them, phosphorus content was the highest. The antioxidant vitamins contained 5.38 ㎍ / RE of vitamin A, 2.63 mg of vitamin C, and 0.3 mg, 0.11 mg and 2.82 mg of vitamin B ₁ , B ₂ and niacin, respectively, of the water soluble vitamins. When consumed 100 g of ginseng in adolescence and old age, intake of phosphorus and iron was 14.3%, phosphorus 45.6%, iron 43.1%, sodium 0.3% and potassium 20.2% . Therefore, the nutrient density of calcium and potassium was high. However, when consumed 100 g of the sun-dried product in adolescence and old age, the consumption ratio of vitamin A, vitamin A, and vitamin C was 0.63% and 1.10%, respectively, and 2.39% and 2.63% . In the intake ratio nutritional recommended dose of water-soluble vitamins, when ingested wire product 100 g of adolescent and old age vitamin B ₁ it was 23.1% and 25.0% vitamin B ₂ are 6.47% and 7.33%, and niacin are 16.6% and 17.6% And vitamin B ₁ and niacin.

<3-2> Analysis of Nutrient Components and Content of Nitric Oxide Prepared by Nutritional Label

The sugar content in the nutrient components (calories, protein, fat, carbohydrate, sodium, cholesterol, simple sugar, trans fat, saturated fat) was measured by HPLC (Water Breeze System, USA), Sodium is ICP (IRIS Intrepid II XSP, USA), Protein is an automatic crude protein analyzer (Foss Kjeltec 2300, Sweden), Fat is Soxhlet extraction tube and Cholesterol, Trans fat, Fat is GC (Schimadzu 2010, Were used to analyze the nine nutrients. The analyzed nutrients were expressed as total content ratios.

Table 4 shows the results of analyzing the ninth nutrients used in the nutritional labeling agent for the four types of the line type of the present invention manufactured according to the above examples.

All the nutrients of the three cultivars, three cultivars, seven cultivars, and nine cultivars were the same, although the contents of heat, protein, fat, carbohydrate, Therefore, there was no change in nutrient content according to the number of steamed and dried.

<3-3> Analysis of moisture, fiber and ash content

The moisture content was measured with an infrared moisture analyzer (Presisa HA-300, Switzerland) according to the AOAC method. That is, a certain amount of the sample was taken in the measuring dish of the infrared moisture meter and the moisture content was measured. The calculation of the solid water amount was made by subtracting the moisture content from the total sample amount. The ash content was measured by weighing 5 g of the sample in accordance with AACC (08-01) for 4 hours at 580 ° C in a painting furnace.

Table 5 shows the moisture content, fiber content and ash content of the present invention.

Moisture content was 5.69 ~ 5.90%, fiber content was 6.18 ~ 6.69%, and ash content was in the range of 2.5 ~ 2.7%. However, moisture, fiber and hides did not show a tendency proportional to the number of stubbing and drying.

<3-4> Determination of the content of functional components (total polyphenol, flavonoid, anthocyanin)

The content of polyphenol compounds was measured by Folin-Denis method. Each sample was dissolved in distilled water at a concentration of 10 mg / mL. Then, 0.2 mL was added to the test tube and distilled water was added to make 2 mL. Then 0.2 mL of Folin-ciocalteu's phenol reagent was added and mixed well and left at room temperature for 3 minutes . After exactly 3 minutes, 0.4 mL of a saturated solution of Na ₂ CO ₃ was added and mixed. Distilled water was added to make 4 mL, then left at room temperature for 1 hour and absorbance was measured at 725 mm using an absorption spectrophotometer (Hitachi UV-2001, Japan) Respectively. The polyphenol content of the total polyphenol compounds was calculated from the standard curves prepared using tannic acid (Sigma Chemical Co. USA).

Total flavonoid content was measured by the method of Kim et al. As follows. 0.1 mL of 10% Al (NO ₃ ) _2, 0.1 mL of 1.0 M CH ₃ COOK, and 4.3 mL of 80% ethanol were added to 0.5 mL of the 80% ethanol extract solution of each sample in this order. The mixture was thoroughly stirred and left to stand at room temperature for 30 minutes. The absorbance was measured at 415 nm and the total flavonoid content of the sample was converted from the calibration curve of rutin.

Anthocyanin content was determined by adding 0.1 g of each sample to a 50 ml Erlenmeyer flask, adding 5 ml of 1% HCl-99% MeOH solution, extracting 3 times at 4 ° C for 24 hours, and then filtering using Advanta No. 2 (Φ 55 mm) And the solution was diluted to 25 ml with an extraction solvent. The filtrate was filtered through a syringe filter (Whatman 0.2 ㎛ NYL) and used as a test solution for HPLC analysis.

The total flavonoids, polyphenols, and polyphenols, which are functional ingredients in water and ethanol extracts of the twelve curly and fermented seaweeds sold in the market, and of the present invention 1, 3, 3, 7, Phenol and anthocyanin contents are shown in Table 6.

The total flavonoid contents in water extracts were 3.27 ± 0.01 mg / g, 12.5 ± 0.001 mg / g, 5.18 ± 0.00 mg / g, and 5.50 ± 0.20 mg / g, respectively, 7, 7, 9, 9, 9, 11, 12, 13, 14, 11, 14, The tendency was to increase significantly in proportion to the number of dried and dried pods. This tendency was the same in the ethanol extracts, and the total flavonoid contents were higher in the water extracts than in the ethanol extracts.

The content of polyphenols in water extracts was 5.47 ± 0.10 mg / g for the 12 curved lines sold in the market, 5.11 ± 0.04 mg / g for the fermental wire, 9.65 ± 0.02 mg / 12.7 ± 0.04 mg / g, 7 7 cells were 19.7 ± 0.10 mg / g and 9 cells were 23.0 ± 0.57 mg / g. , And the number of 9 - cells increased significantly in proportion to the number of dried and dried sausages. Polyphenol contents were higher in ethanol extracts than water extracts.

The anthocyanin content of water extracts was 0.49 ± 0.00 ㎍ / g for 12 curved lines sold in the market, 0.58 ± 0.00 ㎍ / g for fermented wire, 2.02 ± 0.00 ㎍ / g for 1 - The mean value of the mean values of the mean values of the mean values of the mean values of the mean values of the mean values of the mean values of the mean values And the tendency to increase significantly in proportion to the number of sipping and drying. However, the content of anthocyanin in the ethanol extracts was the highest at 15.13 ± 0.00 ㎍ / g in the fermental wire. Unlike the above trend, the anthocyanin content in the ethanol extract was 11.98 ± 0.00 ㎍ / , 7.95 ± 0.50 ㎍ / g in 7 ㎎ / g, 7 ㎎ in 9 ㎎ / g, and 6.96 짹 0.00 ㎍ / g in 9 ㎎ in 9 cells, respectively.

< Example  4>

The functionality of the present invention Solar Physiological activity  analysis

<4-1> Measurement of electron donating ability

As for the electron donating ability (EDA), 1 mL of 0.2 mM DPPH solution was added to 2 mL of each sample according to the Blois method, mixed in a mixer for 10 seconds, reacted at 37 ° C. for 30 minutes, The absorbance at 517 nm was measured using a spectrophotometer (Hitachi UV-2001, Japan), and the absorbance difference before and after the addition of the sample was expressed as a percentage.

Electron donating ability (%) = [1 - (A - B) / C] x 100

(Where, A: absorbance of sample-added sphere, B: absorbance of blank, C: absorbance of sample-added sphere)

The results of measurement of the electron donating ability for the hydrothermal and 70% ethanol extracts of sunscreen are shown in Table 7 and Table 8, respectively.

Table 7 shows the results of electron donating ability for the hot-water extract of the line type, and the electron donating ability was increased with the increase of the hot-water extract concentration in all the 6-line group (p <0.05). The electron donating ability of 12 curved line extracts was higher than that of fermented wire type. The electron donating ability increased from 1 to 9 to 9, and it was higher than that of other lineal extracts The donor ability was confirmed.

Table 8 shows the results of electron donating ability measurement for the ethanol extract of sunscreen. Similar to the results of the hydrothermal extracts of Table 7, the electron donating ability was increased (p <0.05) as the concentration of ethanol extract increased in all six groups, and the electron donating ability was higher in the 12-curved ethanol extract. In addition, the ethanol extract showed higher electron donating ability than the hot water extract.

<4-2> Measurement of SOD (Superoxide dismutase) -like activity

SOD-like activity was determined by adding 3 mL of tris-HCl buffer (50 mM tris [hydroxymethyl] amino-methane + 10 mM EDTA) and 0.2 mL of 7.2 mM pyrogallol to 0.2 mL of each sample according to Marklund's method Respectively. After incubation at 25 ° C for 10 minutes, the reaction was stopped with 0.1 mL of 1N HCl and the absorbance at 420 nm was measured. The SOD - like activity was expressed as the absorbance reduction ratio between the additive and no additive.

SOD-like activity (%) = [1 - (S - B) / C] 100

(Where S: absorbance of sample-added sphere, B: absorbance of blank, and C: absorbance of sample-added sphere)

The results of the SOD-like activity measurement for the hydrothermal and 70% ethanol extracts of sunscreen are shown in Table 9 and Table 10, respectively.

Table 9 shows the results of the SOD-like activity measurement for the hot water extract of Sunshik. SOD - like activity increased with increasing concentrations of all - line hot - water extracts (p <0.05), and high SOD - like activity was observed in the 12 - curd hot - water extract and 9 - cell 9 - cell extract.

Table 10 shows the results of SOD-like activity measurement of ethanol extract of sunscreen. Similar to the results in Table 9, the SOD-like activity increased with increasing concentrations of all-line ethanolic extracts (p <0.05) .

<4-3> Measurement of reducing power

The reducing power was measured by modifying the method of Wong et al. To 0.5 mL of each extract solution, add 1 mL of 0.2 M phosphate buffer (pH 6.6) and 1 mL of 1% potassium ferricyanide, mix well and incubate at 50 ° C for 30 minutes. After cooling to room temperature, 10% TCAT (ricarboxylic acid cycle) 1 mL of the solution was added and left for 10 minutes. Take 0.5 mL of this solution, add 1 mL of distilled water and 0.5 mL of 0.1% FeCl ₃ , and measure the absorbance at 700 nm. BHT (butylated hydroxytoluene) was used as a control.

Table 11 and Table 12 show the results of measuring the reducing power for the hot water and 70% ethanol extracts of the line type, respectively.

Table 11 shows the results of the measurement of the reducing power of the hot-water extract of Sunshik. Reducing power was increased (p <0.05) as the concentration of hot - water extract increased in all the groups, and 12 - curd hot - water extract and 9 - cell hot - water extract showed high reducing power. Especially, at the concentration of 0.1 mg / ml of extract, the reducing power was similar to that of control BHT.

Table 12 shows the measurement of the reducing power for the ethanol extract of the sunscreen. The concentration of ethanol extract increased with increasing concentration of ethanol extracts (p <0.05). At the concentration of 0.1 mg / ml of extract, 12 lines, 3 cells, 7 cells, 7 cells, 9, 9, and 9 were higher than those of the control. In addition, the ethanol extract showed higher reducing power than the hot water extract.

< Example  5>

The functionality of the present invention Solar  Improvement of metabolic syndrome

<5-1> Experimental animals and experiments

Twelve weeks old SD male rats were fed with solid diet for 2 weeks and then fed for 8 weeks with normal diet (normal group), high fat diet (obesity group), high fat diet + 1 hypertensive diet (1HF) (3HF), high - fat diet + 7HP diet (7HF) and high fat diet + 9HP diet supplemented diet (9HF) were fed.

The experimental composition (Table 13) and the dietary composition (Table 14) for experimental animals are shown in Tables 13 and 14 below, respectively, and the diets used in the experiment were based on the composition of AIN-93 purified diet. High fat diet produced diets containing 20% fat and 1% cholesterol, high in saturated fatty acid content instead of corn oil, and induced obesity, a characteristic of hyperlipidemia and metabolic syndrome. In the experimental group, the above-mentioned four kinds of the wire powders of the present invention were mixed at 10% each in the high-fat type.

<5-2> Measurement of growth condition

Body weight and feed intake were measured at regular intervals once a week, and drinking water was allowed to eat freely. Experimental animals were kept at ambient temperature of 22 ± 1 ℃ and relative humidity of 65 ± 5%, and the darkness was adjusted to 12 hours (09:00 ~ 21:00). Dietary intake and body weight gain were used to determine feed efficiency.

As shown in Table 15 below, the growth of the experimental animals was randomly arranged so that there was no significant difference between all groups. At the end of the experiment, the body weight of the normal diet group was 427.6 ± 6.79 g, While the high - fat diet group showed a 12.9% increase in body weight compared to the normal group. However, in addition to the high fat diet method, the four types of feeding of the present invention showed a decrease in body weight gain, but it did not reach the normal dietary level. Feed efficiency was the lowest in group C, which is a normal diet group, while the high - fat diet was the highest in group HF. At this time, the dietary efficiency increased by the high - fat diet method was significantly lowered at the p <0.05 level by the feeding of four kinds of feed, but it was not at the level of the normal diet. Therefore, the daily weight gain and decreased dietary efficiency increased by the high fat diet compared to the fat diet, but could not be adjusted to the normal level.

As shown in Table 16 below, the weight of the organ in terms of weight per 100 g of the experimental animals was significantly increased at the level of p <0.05 compared with that of the group C of 17.8 ± 0.51 g in the high-fat diet group. The weight of kidneys showed similar weight in all groups. In the experimental group, 3HF group showed a high value of 2.98 ± 0.09 g, while the 7HF group showed a low value of 2.68 ± 0.85 g. There was no significant difference in kidney weight between all groups.

<5-3> Evaluation of obesity index

After 8 weeks of experimental diets, obesity index was evaluated to determine if obesity was induced. The obesity index was measured by body length and weight from the nose to the anus in the experimental animals. The Rohrer index, Lee index, T.M. The body fat content, which is the cause of obesity, was evaluated by T.M. index data were used for the calculation.

- Rohrer index = [{Body weight (g) / Naso-anal length (cm) ³ } 10]

- Lee index = Body weight (g) ^1/3 / Naso-anal length (cm) ³

- TM index = [{Body weight (g) / Naso-anal length (cm) 2.823} × 10 3]

- Body fat content = [0.581 × TM index - 22.03]

The Naso-anal length, Rohrer index, Lee index, TM index, and body fat content of the obesity index measured after 8 weeks of high-fat diets to induce obesity by the experimental diet are shown in Table 17 below.

Naso-anal length was significantly shorter at 26.5 ± 0.16 in group C compared with 25.8 ± 0.20 in the HF group at p <0.05. However, when four kinds of lines were supplied, 26.4 ± 0.28, 26.6 ± 0.20, 26.3 ± 0.24, and 26.8 ± 0.11 cm, respectively, were similar to the Naso-anal length of the normal diet group.

The Rohrer index from the nose to the anus was significantly higher in the HF group (32.6 ± 0.82 cm) than in the C group (26.5 ± 0.44 cm) At this time, the Rohrer index showed a significant decrease at the p <0.05 level in the group fed with 1 st, 1, 3, 3, 7, and 9 groups compared to the HF group, The length did not reach the normal level.

The Lee index is normal for rats less than 300, and is considered to be obese. In this example, the C, 1HF, and 9HF groups were in the normal range, while the remaining groups were in the obesity range. Especially, HF group showed 319.2 ± 2.63 compared to 298.1 ± 1.62 of C group, which showed a 7.1% increase in high fat diet group compared with that of normal diet group. However, the Lee index, which was increased by highland method, could be lowered to the normal level by the supply of 1 - for - 1 and 9 - for - 9. Another researcher reported that the Lee index was higher in the obese mice than in the C57BL / 6J obese mice and lean mice fed the solid diet, as the weight gain ratio of the obese mice was larger.

In the TM index, the criterion for obesity is 55 or more. In this example, the remaining HF and 7HF groups were judged to be obese except for the C group, 1HF group, 3HF group and 9HF group. Therefore, obesity by TM index evaluation by high - fat diet could be controlled by the supply of 1, 1, 3, 9 and 9 - diet.

Body fat content in the body was judged to be obesity when the body fat content was 10 or more. In this example, body fat content in C group was lowest at 5.47 ± 0.42 g per 100 g body weight, and body fat content in HF group was found to be 11.6 ± 0.80 g . The body fat contents of the groups fed with 1, 1, 3, 7, 7, 9, and 9 groups were 6.99 ± 0.78, 6.87 ± 0.63, 7.47 ± 0.53, and 5.81 ± 0.67, respectively, 0.05 level, similar to that of the normal diet group.

Therefore, in this example, the obesity index was evaluated in the obesity index evaluation of the experimental animals that consumed the high fat diet group compared to the normal diet group. However, there was no agreement in all obesity indices when the above four kinds of diets were supplied, but the obesity index increased by high fat diet was somewhat controlled.

<5-4> Sample collection

The rats fed the experimental diet were fasted for 12 hours and anesthetized with CO. After the laparotomy, blood was collected from the abdominal vena cava. After incubation at room temperature for 1 hour, the serum was separated by centrifugation at 3000 rpm for 15 minutes and stored at -70 ° C. until analysis. Liver tissue was perfused with 1.15% KCl buffer solution and extracted. After washing several times to remove moisture, the weight was measured. The kidney and spleen tissues were weighed after the water was completely removed.

<5-5> Analysis of hematological composition

Hemoglobin concentration and hematocrit value as anemia index were measured using Hemochroma reader (Boditech, Korea). The total protein and albumin content in the serum was measured with a kit (Asan, Korea), and the globulin content was calculated as the limiting value of the albumin content in the total protein content.

Hemoglobin and hematocrit levels are shown in Table 18 below.

The hemoglobin concentration was 6.84 ± 0.57 in the C group, 8.16 ± 0.65 in the HF group, 8.16 ± 0.65 in the HF group, 7.68 ± 0.88 in the 3HF group, 8.69 ± 0.91 in the 7HF group, and 7.55 ± 0.81 in the 9HF group And there was no significant difference between all groups. The hematocrit level was 20.5 ± 1.72% in the C group, 24.5 ± 1.94% in the HF group, 24.5 ± 2.89% in the 1HF group, 23.03 ± 2.63% in the 3HF group, 26.1 ± 2.72% in the 7HF group and 22.7 ± 2.43% There was no significant difference between all groups.

The total protein, albumin, and globulin content in the serum are shown in Table 19. The total protein content was 14.4 ± 0.28 g / dl in the C group, 15.2 ± 0.60 g / dl in the HF group, 14.8 ± 0.65 g / dl in the 1HF group, 14.1 ± 0.38 g / dl in the 3HF group, 12.0 ± 0.44 g / dl in the 7HF group, 9HF group 11.9 ± 2.30 g / dl. There was no significant difference among all groups. However, the content of albumin was 3.61 ± 0.22 g / dl in group C, 2.92 ± 0.36 g / dl in the HF group, 2.82 ± 0.32 g / dl in the 1HF group and 2.65 ± 0.20 g / dl in the 3HF group, dl and 9HF group 1.75 ± 0.31 g / dl, respectively. There was no significant difference in globulin content among all experimental groups.

<5-6> Indices related to diabetes

Serum glucose content was measured by blood glucose meter (Accucheck, Germany) after blood was collected from the tail vein after fasting for 12 hours or more. The blood glucose level of the experimental animals was significantly higher than that of the C group (105.3 ± 3.13 mg / dl) and the HF group (112.9 ± 5.40 mg / dl, p <0.05) As a result, there was no significant difference in the concentrations of 97.6 ± 5.88, 94.5 ± 4.25, 95.0 ± 4.03, and 99.1 ± 5.08 mg / dl, respectively (FIG. 2).

Insulin concentration was measured using an ELISA kit, and insulin resistance and insulin secretion were measured according to a predetermined formula. As shown in FIG. 3 (a), the insulin concentration in the experimental animals was higher in the HF group (1.78 ± 0.03 ng / ml), the 1HF group (1.62 ± 0.04 ng / ml) (P <0.05) in both the 3HF group (1.61 ± 0.02 ng / ml), the 7HF group (1.57 ± 0.02 ng / ml) and the 9HF group (1.49 ± 0.02 ng / ml). However, as shown in Fig. 3 (b), the insulin resistance (HOMA-IR) was higher than that in the group fed HF (8.75 ± 0.60) (P <0.05) in both HF (6.74 ± 0.30), 3HF (6.73 ± 0.29), and 7HF (6.62 ± 0.29) and 9HF (6.59 ± 0.35) were lowered to the normal level there was. As shown in FIG. 3 (c), the HF group (0.35 ± 0.03), the 1HF group (0.35 ± 0.02), and the 3HF (0.36 ± 0.02) were lower in the insulin secretion ability (HOMA- , 7HF (0.35 ± 0.02), and 9HF (0.32 ± 0.01) were significantly lower at the p <0.05 level. Therefore, in this experiment, the metabolic syndrome index by high fat diet was higher than that of the normal diet, but the blood glucose and insulin resistance were significantly higher but the insulin secretion was lower. However, at this time, the supply of four kinds of lines seems to control glucose and insulin resistance somewhat.

Adiponectin and leptin concentrations were analyzed using an ELISA assay kit.

The concentration of adiponectin according to the ingestion of the rodents in the experimental animals is shown in Fig. 4 (a). The HF group (1.82 ± 0.19 ng / ml) was significantly higher at the p <0.05 level than the C group (1.10 ± 0.03 ng / ml). However, the levels of adiponectin increased by the high-fat diet were higher in the 1HF group (1.09 ± 0.83 ng / ml), the 3HF group (1.01 ± 0.04 ng / ml), the 7HF group (1.07 ± 0.05 ng / ml) ng / ml). Among adipocytes secreted by adipocytes, adiponectin is expressed in the adipocyte differentiation process, and when it is obese, the content of adiponectin in blood is high. In addition, adiponectin has been reported to increase insulin sensitivity in muscle and liver tissue and also to oxidize fat.

The leptin concentration was significantly higher at the p <0.05 level in the HF group (2.56 ± 0.05 ng / ml) as compared to the C group (1.99 ± 0.02 ng / ml) Respectively. However, p <0.05 was found in the group 1HF (1.78 ± 0.04 ng / ml), 3HF (1.81 ± 0.05 ng / ml) and 7HF (1.86 ± 0.06 ng / At the 0.05 level, the concentration of leptin could be lowered to the level of the normal diet. The decrease in leptin concentration is associated with the decrease in body fat, so that the leptin concentration is also significantly decreased as the fat amount is decreased.

<5-7> Indications of complications related to hyperlipemia

The activity of glutamic oxaloacetate transaminase (GOT) and glutamic pyruvate transaminase (GPT) in the serum was measured using the kit for measuring GOT and GPT activity (Asan Pharmaceutical, Korea) by the Retiman-Frankel method.

Figure 5 (a) shows the GOT activity measured in order to observe the degree of liver damage caused by the high-fat diet method. The GOT activity of C group was 127.1 ± 3.24 karmen / ml, which was the highest in all groups, while the GOT activity of HF group was 91.3 ± 9.62 krmen / ml, and the HF group (84.3 ± 7.81 karmen / ml) (81.7 ± 5.06 krmen / ml), respectively. GPT activity was 153.4 ± 2.55 karmen / ml in group C, 137.5 ± 9.59 karmen / ml in HF group, 81.2 ± 5.06 karmen / ml in 3HF group and 141.8 ± 9.96 There was no significant difference between all groups in the karmen / ml, 9HF group, 138.2 ± 12.9 karmen / ml.

Liver damage is usually accompanied by hyperlipidemia, increased liver weight, and changes in serum GOT and GPT activity are indicators of liver damage. Changes in the activity of GOT and GPT enzymes are indicative of hepatic tissue damage as hepatocyte degeneration and necrosis progress and transaminase levels become higher in the blood.

Bilirubin content in serum is determined by Evelyn-Malloy method. Direct and indirect bilirubin in serum react with 2,5-dichlorophenyldiazonium under acidic conditions to form azo-bilirubin. The azo-bilirubin formed was measured with a kit. Sulfonic acid reacts with sodium nitrate to form diazo-sulfanilic acid. Direct serum bilirubin reacts specifically with diazo-sulfanilic acid to quantify the amount of azo-bilirubin. Indirect bilirubin was obtained by subtracting the amount of bilirubin directly from total bilirubin.

As shown in FIG. 6 (a), the total bilirubin content of the experimental animals was 0.83 ± 0.06 mg / dL, which was significantly higher at the p <0.05 level than the 0.64 ± 0.01 mg / dL of the C group and the HF group. Compared with the HF group, the levels of HF, 3HF, 7HF, and 9HF were 0.70 ± 0.05, 0.71 ± 0.62, 0.66 ± 0.02, and 0.63 ± 0.09 mg / dL, respectively. Direct and indirect bilirubin were not significantly different in all experimental groups as shown in Figures 6 (b) and 6 (c).

The neutral lipid content in the plasma was measured by using a kit for measuring neutral lipids (Asan Pharmaceutical, Korea) prepared by glycerol-3-phosphate oxidase-PAP enzymatic method. The total cholesterol content was cholesterol hydrolase and cholesterol and free fatty acid (Asan Pharmaceutical Co., Ltd., Korea). The phospholipid content was measured using a kit prepared by an enzymatic method, and the HDL-cholesterol content was measured using a kit (Shin Yang Pharm, Korea) for quantitatively measuring the HDL-cholesterol in the upper layer after precipitating LDL-cholesterol. LDL-cholesterol was calculated by the Friedwald method LDL-cholesterol = (total cholesterol) - [(HDL-cholesterol) + (triglyceride / 5)]. The total lipid content in liver tissue was measured by Folch and other methods using chloroform: methanol (2: 1, v / v), and triglycerides were measured by Mendez et al. and 2.0 ml of isopropanol solution was used as a sample, and triolein was used as a standard solution. Total cholesterol was measured by Zlatkis and Zak method. The phospholipid content in the liver tissue was analyzed by Eng and Noble method using a mixture of 3.0 ml of glacial acetic acid and some of the total lipid components as a sample. The standard reagent was prepared using KH ₂ PO ₄ .

The results of lipid content in serum are shown in Table 20.

The triglyceride content of high fat diet group, 3HF group and 7HF group were higher in experimental group. However, the increase of triglyceride content due to high - fat diet could be reduced by the supply of 9 - diet 9 - meal diet. Total cholesterol contents were not significantly different among all groups. The levels of HDL-cholesterol, which protects against cardiovascular disease, were highest in the normal diet group and significantly higher in the HF, 3HF, 7HF and 9HF groups.

LDL-cholesterol content was lowest in the 9HF group fed with 9-diet and 9HF, followed by C, 3HF and 7HF. The highest group was the HF group. In conclusion, the effects of the high-fat diet on the high-fat diet showed a protective effect on the supply of 9-fetoprotein in the neutral lipid and in the 3-fetoprotein and 9-fetoprotein on the LDL-cholesterol.

The results of lipid content analysis in liver tissues are shown in Table 21.

Total lipid content was significantly increased in HF, 1HF, 3HF, 7HF and 9HF groups compared to C group. The phospholipid content was also significantly higher in the high fat diet group than in the C group. In addition, the neutral lipid was significantly increased in the HF group compared to the C group by the high fat diet. However, it was possible to lower the neutral lipid content in the liver tissue by the high - fat diet by the supply of four kinds of fishes, though it is not significant. Cholesterol content was also significantly increased in the HF group compared to the C group. However, it was not significant, but it was possible to lower the cholesterol content in the liver tissues increased by the high fat diet by the supply of four kinds of lines.

Statistical analysis

The results of all experiments were analyzed using the SPSS statistical package (version 14.0) and the results were expressed as mean ± standard error. The test results were analyzed by one way analysis of variance followed by Duncan's multiple range test to determine the statistical significance of the mean values of each test group at the α = 0.05 level.

Claims (13)

(a) Wheat, bean, white crustacea, burdock, red bean, black rice, brown rice, glutinous rice barley brown rice, sorghum, corn, caramel, sweet potato, apple, strawberry, pumpkin, potato, citrus, yulmu, stevia, burdock, , Rootstocks and spinach, after washing;
(b) growing the materials; And
(c) cooling the donated materials. The method according to claim 1,
Wherein the materials of step (a) have the following composition ratios:

. The method according to claim 1,
Wherein the step (a) is performed at room temperature for 14 hours. The method according to claim 1,
Wherein the step (b) is performed at a temperature of 115 ° C or higher for 2 hours and 30 minutes using a large-capacity hot pot or at a temperature of 105 ° C or higher for 50 minutes using a small-capacity hot pot. 5. The method of claim 4,
Wherein the step (c) comprises discharging the heat at room temperature until the internal temperature reaches 75 to 85 占 폚. 6. The method of claim 5,
Wherein the step (c) is performed for 10 minutes. The method according to claim 1,
Wherein the steps (b) and (c) are further performed twice, six or eight times. The method according to claim 1,
(d) drying the cooled materials;
(e) roasting the dried materials; And
(f) milling the roasted ingredients. 9. The method of claim 8,
Wherein the moisture content of the materials dried in step (d) is less than or equal to 10% by weight. 10. The method of claim 9,
Wherein the step (d) is performed at 60 DEG C for 40 hours using a drier. 9. The method of claim 8,
Wherein the step (e) is carried out at 90 to 95 캜 for 6 to 7 minutes using a roasting pot. 12. A functional wire made according to the method of any one of claims 1 to 11. 13. The method of claim 12,
Wherein the functional line is effective for preventing or symptomatic improvement of the metabolic syndrome.

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