KR102542995B1 - Composition for anti-oxidation, anti-inflammation, anti-bacterial containing an extraction of backhousia citriodora, liriopsis tuber and angelica gigas - Google Patents

Abstract

The present invention relates to a composition of a mixed extract of lemon myrtle, corticosteroid, and Angelica quai, and the mixed extract of lemon myrtle, corticosteroid, and angelica quai has excellent DPPH radical scavenging activity and lipid peroxidation inhibitory effect, and is effective against Staphylococcus aureus , epidermal staphylococcus Cocci ( Staphylococcus epidermidis ) and Listeria monocytogenes ( Listeria monocytogenes ) Represents an antibacterial effect, effectively inhibits inflammation, and also has an excellent anti-inflammatory effect.
Therefore, the mixed extracts of lemon myrtle, rhubarb, and Angelica quai can be used in medicines, foods, cosmetics, etc. for antioxidant, anti-inflammatory, or/and antibacterial purposes. In addition, it can be used as an anti-pollution material by utilizing antioxidant and anti-inflammatory effects.

Description

Composition for anti-oxidation, anti-inflammation, anti-bacterial containing an extraction of backhousia citriodora, liriopsis tuber and angelica gigas}

The present invention relates to a composition comprising a mixture of extracts of lemon myrtle, rhubarb, and angelica quasi.

In addition, the mixed extracts of lemon myrtle, rhubarb, and angelica quasi can reduce oxidative stress and inflammation caused by fine dust by suppressing oxidative stress and inflammation, so it can be used as an anti-pollution food composition.

Fine dust collectively refers to dust with a particle size of 10㎛ or less, abbreviated as PM ₁₀ (Particulate Matter 10), and is classified as PM _2.5 (Particulate Matter 2.5), which is ultra-fine dust with a particle size of 2.5㎛ or less. It is mainly generated when fossil fuels are burned, and fine dust from China, which relies on coal for 70%, accounts for a large part. In addition, yellow dust refers to a phenomenon in which small sand, ocher, or dust from deserts and loess regions in the center of the Asian continent, such as China and Mongolia, floats in the sky and is blown away by the wind from the upper layers. this is possible

In particular, when fine dust comes in direct contact with the skin, which is located on the outermost part of our body, the skin acts as a barrier function between the organism and the environment. cause problems In particular, since fine dust is 20 times smaller than pores, it can easily penetrate the human skin. For example, in the skin, which is a major site for absorbing environmental pollutants, absorption of a toxic substance such as chloroform through the skin is considered equivalent to inhalation through the respiratory tract.

Fine dust causes oxidative stress and inflammation in the body or skin. The induced inflammation affects the whole body and increases the risk of skin inflammatory diseases, hair loss, atopic aggravation, stroke, brain inflammatory diseases such as Alzheimer's disease, respiratory diseases, and cardiovascular diseases.

Currently, the demand for anti-inflammatory and antioxidant functional materials that protect against inflammatory reactions and oxidative stress caused by stimuli such as fine dust is rapidly increasing.

Currently, many studies are being conducted to find new materials with high therapeutic effects and low side effects from materials derived from natural products whose safety has been proven.

In the present invention, a natural product-derived composite material was developed that enhances antioxidant activity in the body, reduces oxidative stress and inflammation, and exhibits antibacterial effects against microorganisms, and it was confirmed that the composite material has excellent antioxidant, inflammatory, and antibacterial effects without cytotoxicity. By doing so, the present invention was completed.

Korean Registered Patent No. 10-1851603

An object to be solved by the present invention is to provide an antioxidant, anti-inflammatory, and antibacterial composition containing mixed extracts of lemon myrtle, rhubarb, and angelica quasi as an active ingredient. include

In addition, another object of the present invention is to provide a food composition for anti-pollution comprising a mixed extract of lemon myrtle, coriander and Angelica quai as an active ingredient.

In order to achieve the above object, the present invention provides an antioxidant, anti-inflammatory or antibacterial food composition comprising a mixture of extracts of lemon myrtle, rhubarb, and Angelica quai as an active ingredient.

In the present invention, the mixed extract may be extracted by mixing lemon myrtle, coriander, and Angelica gigas in a weight ratio of 1 to 3: 4 to 6: 2 to 4.

In the present invention, the mixed extract may be hot-water extraction of lemon myrtle, puerperium chinensis, and Angelica gigas.

In the present invention, the antibacterial agent may inhibit the growth of one or more microorganisms selected from the group consisting of Staphylococcus aureus , Staphylococcus epidermidis , and Listeria monocytogenes .

In addition, the present invention provides a cosmetic composition for antioxidation, anti-inflammatory or antibacterial use comprising a mixed extract of lemon myrtle, rhubarb, and Angelica quai as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for antibacterial use or prevention or treatment of inflammatory diseases, comprising a mixed extract of lemon myrtle, rhubarb, and Angelica quai as an active ingredient.

In the present invention, the inflammatory disease may be a brain or neuroinflammatory disease.

In addition, the present invention provides a quasi-drug composition for antioxidant, anti-inflammatory, or antibacterial use comprising a mixed extract of lemon myrtle, rhubarb, and angelica quasi as an active ingredient.

In addition, the present invention provides a food composition for anti-pollution comprising a mixture of extracts of lemon myrtle, rhubarb, and Angelica quai as an active ingredient.

In the present invention, the anti-pollution may be to suppress inflammation or oxidative stress induced by yellow dust or fine dust.

The present invention relates to an antioxidative, anti-inflammatory, and antibacterial composition comprising a mixed extract of lemon myrtle, rhubarb, and Angelica quai. Mixed extracts of lemon myrtle, rhubarb, and Angelica quai have excellent DPPH radical scavenging activity and lipid peroxidation inhibitory effects, and are effective against Staphylococcus aureus, Staphylococcus epidermidis , and Listeria monocytogenes . It has been confirmed that it exhibits an antibacterial effect and effectively inhibits inflammation, so it can be used for antioxidant, anti-inflammatory and antibacterial purposes.

In addition, mixed extracts of lemon myrtle, coriander, and Angelica quai can be used to develop anti-pollution foods that alleviate oxidative stress and inflammation caused by fine dust.

1 is a result of analyzing the antibacterial effect of the mixed extracts of lemon myrtle, rhubarb, and Angelica quai by concentration for each strain.
Figure 2 is the result of analyzing the DPPH radical scavenging activity according to the concentration of the mixed extract (LMD) of lemon myrtle, rhubarb and angelica.
Figure 3 is a result of analysis of the change in the production of lipid peroxide (MDA), an indicator of oxidative damage, by administration of a mixed extract (LMD) of lemon myrtle, pulmona sinensis, and Angelica quai after inducing brain inflammation in mice with LPS.
Figure 4 shows changes (increase/decrease) of microglial cells and astrocytes, which are inflammation-related cells, in brain tissue by administration of a mixed extract (LMD) of lemon myrtle, rhubarb, and Angelica quai after inducing brain inflammation in mice with LPS. This is the result of immunostaining analysis.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein and the experimental methods described below are those well known and commonly used in the art.

In one aspect, the present invention relates to a food composition for antioxidant, anti-inflammatory, or antibacterial use comprising a mixed extract of lemon myrtle, rhubarb, and Angelica quai as an active ingredient.

The food composition for antioxidant, anti-inflammatory or antibacterial use of the present invention includes a mixture of extracts of lemon myrtle, coriander, and angelica quasi as an active ingredient.

In the present invention, "lemon myrtle" is a plant of the scientific name Backhousia citriodora, and is a shrub mainly inhabiting tropical forests in Queensland, Australia.

In the present invention, "Macmundong" is a plant with the scientific name Liriope platyphylla , which grows well in shaded and humid places, and is a perennial herbaceous plant belonging to the Liliaceae family that blooms purple flowers in July and bears black fruits in October.

In the present invention, "Angelica" is a plant whose scientific name is Angelica gigas Nakai , and is also called Angelica gigas. It is a dried root of Angelica gigas, a perennial herb belonging to the Umbelaceae, and is characterized by sweet and spicy taste.

In the present invention, "extract" refers to an extract obtained by extraction treatment, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a crude or purified product of the extract, or a mixture thereof, etc. It includes extracts of all formulations that can be formed by

In the present invention, the extract may be processed by a known method to the extent that useful components are not destroyed from dried plants, for example, by natural drying in the shade. In addition, crushing or pulverization may be pulverized by crushing or pulverizing to the extent that useful components of the plant can be sufficiently extracted in the subsequent extraction process. The drying and crushing or crushing process may be carried out in reverse order or repeatedly if necessary.

The extract may be prepared according to a conventional plant extract manufacturing method, and specifically, may be a solvent extraction method, water immersion extraction method, cold needle extraction method, warm needle extraction method, or ultrasonic extraction method. available.

The extraction solvent of the extract may be water or an organic solvent. The organic solvent may be a polar solvent, a non-polar solvent, or a mixture thereof, and may be, for example, alcohol, alcohol dilution water, hexane, methylene chloride, acetone, ethyl acetate, ethyl ether, chloroform, or a mixture thereof, and the alcohol may be a C ₁ to C ₅ alcohol, for example methanol, ethanol, propanol, butanol, isopropanol, and the like. In addition, the alcohol dilution water may be diluted with water to 50 to 99.9% (v / v) of alcohol.

In addition, the extract extracted with the solvent may be further subjected to a fractionation process with any one solvent selected from the group consisting of hexane, methylene chloride, acetone, ethyl acetate, ethyl ether, chloroform, water, and mixtures thereof. At the time of the fractionation, two or more solvents may be used, and each solvent fraction may be prepared by sequentially using or mixing according to the polarity of the solvent.

The prepared extract or the fraction obtained by performing the fractionation process may then be filtered or concentrated or dried to remove the solvent, and filtration, concentration, and drying may all be performed. Specifically, the filtration may be performed using a filter paper or a vacuum filter, the concentration may be concentrated under reduced pressure using a vacuum concentrator, for example, a rotary evaporator, and the drying may be performed, for example, by a lyophilization method.

In the present invention, the "mixed extract" is an extract prepared by extracting lemon myrtle, angelica angelica, and lemon myrtle, prepared by extracting and mixing lemon myrtle, angelica angelica, and lemon myrtle, respectively, or by mixing lemon myrtle, angelica angelica, and angelica then extracting. may have been

In one embodiment of the present invention, after mixing dried lemon myrtle leaves, rhubarb, and Angelica quai, mixed extraction was performed for 3 hours using a pressurized extract using distilled water as a solvent at 100 ° C.

Therefore, the mixed extract of the present invention is obtained by hot water extraction of lemon myrtle, rhubarb, and Angelica quai, and specifically, may be obtained by hot water extraction at 90 to 110 ° C.

In one embodiment of the present invention, an extract was prepared by mixing lemon myrtle, angelica lucifera, and angelica quai at a weight ratio of 2: 1 to 10: 3, and the result of analyzing the cytotoxicity of the extract according to the mixing ratio of lemon myrtle, angelica lucidum, and angelica quai , Lemon myrtle, Mcmundong and Dong Quai 2: 5: 3 (Example 1), the mixed extract did not show cytotoxicity, but lemon myrtle, Mc Mundong and Dong quai 2: 1: 3 (Comparative Example 1) or When mixed at a weight ratio of 2: 10: 3 (Comparative Example 2), cytotoxicity was high.

Therefore, in the present invention, the mixed extract may be extracted by mixing lemon myrtle, angelica parsnips, and angelica in a weight ratio of 1-3: 4-6: 2-4. It may be prepared by mixing in a weight ratio of 6: 2 to 4 and hot water extraction at 90 to 110 ° C.

In one embodiment of the present invention , Staphylococcus aureus , Staphylococcus epidermidis , Listeria monocytogenes , or The antibacterial effect against Escherichia coli was analyzed.

As a result of the analysis, antibacterial effects were shown against Staphylococcus aureus, Staphylococcus epidermidis , and Listeria monocytogenes , and no antibacterial effect was confirmed against Escherichia coli . .

In addition, for Staphylococcus aureus and Staphylococcus epidermidis , clear zones were clearly observed even when the diluted sample was treated at a concentration of 1%, and the mixed extract of lemon myrtle, rhubarb, and angelica quasi ( Staphylococcus aureus ) and epidermal staphylococcus ( Staphylococcus epidermidis ) It was confirmed that it exhibits very excellent antibacterial activity.

Therefore, the mixed extracts of lemon myrtle, coriander, and Angelica chinensis inhibit the production of microorganisms, and thus can be used as an antibacterial composition.

In the present invention, "antibacterial" refers to inhibiting the growth of microorganisms, specifically, one selected from the group consisting of Staphylococcus aureus, Staphylococcus epidermidis , and Listeria monocytogenes . It may be to inhibit the growth of the above microorganisms.

In one embodiment of the present invention, it was confirmed that DPPH scavenging activity increased in a concentration-dependent manner of the mixed extracts of lemon myrtle, rhubarb, and Angelica quai (Example 1, LMD), and brain inflammation was induced in mice with LPS to analyze the antioxidant effect. As a result of analyzing the change in the production of lipid peroxide (MDA), which is an indicator of oxidative damage, by the administration of the mixed extracts (LMD) of lemon myrtle, rhubarb, and Angelica quai, LPS reduced the amount of MDA produced in the brains of experimental animals by 170%. Increased. This means that oxidative damage was induced by LPS. It was confirmed that the high-dose administration (1000 mg/kg) of the mixed extracts (LMD) of lemon myrtle, rhubarb, and angelica quasi statistically significantly reduced the amount of MDA produced by LPS.

In other words, it was confirmed that the mixed extracts of lemon myrtle, miraculous dong quai and angel quai exhibit DPPH scavenging activity and have excellent antioxidant effects by inhibiting lipid peroxide (MDA) generated by oxidative damage. The mixed extract of can be used as an antioxidant composition.

In the present invention, "antioxidation" may be to protect cells from oxidative stress, specifically inhibiting the production of reactive oxygen species or lipid peroxide production.

In another embodiment of the present invention, in order to analyze the anti-inflammatory effect of the mixed extract (LMD) of lemon myrtle, rhubarb, and Angelica quai, brain inflammation was induced in mice with LPS, and then the mixed extract (LMD) of lemon myrtle, rhubarb, and angelica quai was treated for 7 days. ) After oral administration, the degree of increase in microglia and astrocytes, which are inflammation-related cells, in brain tissue was analyzed. As a result of the analysis, LPS induced an increase in GFAP-positive cells, a marker for astrocytes, and Iba-1-positive cells, a marker for microglial cells, in the brains of experimental animals. It was confirmed that the LPS-induced increase in astrocytes (GFAP-positive cells) and microglial cells (Iba-1-positive cells) was significantly reduced when oral administration of the mixed extracts (LMD) of lemon myrtle, rhubarb, and angelica quasi was administered.

That is, as it was confirmed that the mixed extract of lemon myrtle, corkmundong, and Angelica quai exhibited anti-inflammatory effects, the mixed extract of lemon myrtle, corticosteroid, and angelica quai of the present invention can be used as an anti-inflammatory composition.

In the present invention, "anti-inflammatory" is to inhibit or prevent the occurrence of inflammation, or to protect cells from damage caused by inflammation.

In the present invention, inflammation may be caused by microbial infection, exposure to yellow dust or fine dust, exposure to chemicals, or oxidative stress, but is not limited thereto.

The food composition of the present invention may include pills, powders, granules, infusions, tablets, capsules, liquids, etc., and there is no difference in the type of food to which the mixed extract of lemon myrtle, coriander, and angelica quasi of the present invention can be added. There is no limitation, and there are, for example, various beverages, chewing gum, tea, vitamin complexes, health supplements, and the like.

In the food composition, other ingredients may be added in addition to the mixed extract of lemon myrtle, rhubarb, and Angelica quai, and the type thereof is not particularly limited. For example, it may contain various herbal extracts, food additives or natural carbohydrates, etc., as additional components, as in conventional foods, but are not limited thereto.

In the present invention, "food supplement additive" refers to a component that can be added to food supplementally, and can be appropriately selected and used by those skilled in the art as being added to prepare health functional foods of each formulation. Examples of food additives include various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and fillers, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners , pH regulators, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, etc. are included, but the types of food additives of the present invention are not limited by the above examples.

Examples of the natural carbohydrate include monosaccharides such as glucose and fructose; disaccharides such as maltose and sucrose; and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (thaumatin, etc.), stevia extract (rebaudioside A, glycir hijin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can advantageously be used.

There is no particular limitation on the type of food. Examples of foods to which the above substances can be added include meat, sausages, bread, chocolates, candies, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice creams, various soups, beverages, tea, drinks, There are alcoholic beverages and vitamin complexes.

The food composition may be a health functional food composition.

In the present invention, "health functional food" refers to food manufactured and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functionality for the human body. Here, functional means obtaining useful effects for health purposes such as adjusting nutrients for the structure and function of the human body or physiological functions. The health functional food of the present invention can be prepared by a method commonly used in the art, and can be prepared by adding raw materials and components commonly added in the art during the preparation. In addition, unlike general drugs, there is an advantage in that there is no side effect that may occur when taking a drug for a long time by using food as a raw material, and it can be excellent in portability.

The health functional food has the advantage of having a more excellent effect by ingesting it in the form of inner beauty food. The inner beauty refers to food referred to as 'eating cosmetics or beauty food', and refers to food that changes the skin constitution to be healthy by absorbing various ingredients good for the skin into the body. You can select and consume the inner beauty food that suits your individual skin condition and lifestyle. More preferably, when the cosmetic containing the mixed extract of lemon myrtle, corkmundong, and Angelica quai is mixed with the inner beauty food containing the mixed extract of lemon myrtle, corticosteroid, and angelica quai, antioxidant, anti-inflammatory or It has the advantage of being able to see more effective anti-oxidation, anti-inflammatory or anti-bacterial effects as the antibacterial effect is significantly increased.

The mixing amount of the active ingredient may be appropriately determined according to the purpose of use (prevention, health or therapeutic treatment). In general, when preparing food, the mixed extract of lemon myrtle, coriander, and Angelica quai of the present invention may be added in an amount of 1 to 50% by weight, preferably 5 to 10% by weight of the raw material composition, but is not limited thereto no. However, in the case of long-term intake for the purpose of health and hygiene or health control, the amount below the above range may be used.

In another aspect, the present invention relates to a cosmetic composition for antioxidant, anti-inflammatory, or antibacterial use comprising a mixture of extracts of lemon myrtle, rhubarb, and Angelica quai as an active ingredient.

In the present invention, descriptions of lemon myrtle, mulberry, angelica, mixed extract, antioxidant, anti-inflammatory, and antibacterial properties are as described above.

The cosmetic composition of the present invention may further include compounds or natural extracts known to have antioxidant, anti-inflammatory or antibacterial effects so as to enhance or reinforce antioxidant, anti-inflammatory or antibacterial effects, in addition to the mixed extract of lemon myrtle, rhubarb, and Angelica quai.

In the cosmetic composition for antioxidant, anti-inflammatory or antibacterial use of the present invention, the active ingredient may be included in any amount (effective amount) according to the purpose of use, formulation, blending, etc., as long as it can exhibit antioxidant, anti-inflammatory or antibacterial effects. An effective amount of phosphorus may be included within the range of 0.001% by weight to 99.99% by weight based on the total weight of the composition. Here, "effective amount" refers to the amount of an active ingredient capable of inducing antioxidant, anti-inflammatory or antibacterial effects. Such an effective amount can be determined empirically within the ordinary skill of the skilled artisan.

The cosmetic composition of the present invention may be prepared in various forms, for example, the cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, a solution, suspension, paste, gel, cream , Lotion, powder, cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray may be formulated, but is not limited thereto. In addition, specifically, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, moisture cream, hand cream, essence, nutrient essence, pack, soap, shampoo, It may have a formulation selected from the group consisting of cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, lipstick, makeup base, foundation, press powder, and loose powder, but is not limited thereto.

The cosmetic composition of the present invention may include a carrier acceptable in cosmetic formulations in addition to the active ingredient. Here, "acceptable carrier in cosmetic formulations" is a compound or composition that is already known and used that can be included in cosmetic formulations, or a compound or composition to be developed in the future, which does not have toxicity, instability, or irritation more than the human body can adapt to when in contact with the skin. say that The carrier may be included in the cosmetic composition of the present invention in an amount of about 1% to about 99.99% by weight, preferably about 5% to about 99% by weight, based on the total weight of the composition. However, since the ratio varies depending on the formulation of the cosmetic composition of the present invention and its specific application area (face or hand) or its preferred application amount, the ratio is intended to limit the scope of the present invention in any aspect. should not be understood

Meanwhile, as the carrier, alcohol, oil, surfactant, fatty acid, silicone oil, humectant, humectant, viscosity modifier, emulsion, stabilizer, sunscreen, coloring agent, fragrance, and the like may be exemplified. Alcohols, oils, surfactants, fatty acids, silicone oils, humectants, humectants, viscosity modifiers, emulsions, stabilizers, sunscreens, coloring agents, compounds/compositions that can be used as fragrances, etc. that can be used as the carrier are already known in the art. Therefore, those skilled in the art can select and use an appropriate material/composition.

In another aspect, the present invention relates to a pharmaceutical composition for antibacterial use or prevention or treatment of inflammatory diseases, comprising a mixed extract of lemon myrtle, rhubarb, and Angelica quai as an active ingredient.

In the present invention, the description of the lemon myrtle, rhubarb, angelica, and mixed extracts is as described above.

In the present invention, the antibacterial pharmaceutical composition is a pharmaceutical composition for preventing or treating infectious diseases by inhibiting the growth or promotion of microorganisms.

In the present invention, "infectious disease" is a disease caused by microbial infection, specifically, Staphylococcus aureus , Staphylococcus epidermidis , and Listeria monocytogenes caused by infection. means disease.

In the above-described examples of the present invention, the mixed extracts of lemon myrtle, rhubarb and angelica quasi have antibacterial activity against Staphylococcus aureus , Staphylococcus epidermidis , and Listeria monocytogenes . As confirmed, the pharmaceutical composition of the present invention inhibits the growth of Staphylococcus aureus , Staphylococcus epidermidis , and Listeria monocytogenes , thereby exhibiting an effect of preventing or treating infectious diseases.

In the present invention, "inflammatory disease" is a disease caused by an inflammatory reaction (inflammation), the inflammation is induced by microbial (bacterial, viral, fungal) infection, yellow dust, fine dust, exposure to chemicals or oxidative stress It may be, but is not limited thereto.

The inflammatory disease is allergic disease, inflammatory bowel disease, atherosclerosis, inflammatory collagen blood vessel disease, glomerulonephritis, inflammatory skin disease, sarcoidosis, retinitis, gastritis, hepatitis, enteritis, arthritis, tonsillitis, sore throat, bronchitis, pneumonia, pancreatitis, It may be one selected from the group consisting of sepsis, brain or neuroinflammatory diseases, and nephritis.

In the foregoing example of the present invention, it was confirmed that the mixed extract (LMD) of lemon myrtle, rhubarb, and angelica quai suppressed the increase in the production of astrocytes and microglial cells, which are inflammation-related cells, in the brains of laboratory animals in which inflammation was induced by LPS. As a result, the mixed extracts of lemon myrtle, rhubarb, and angelica can be used to prevent or treat inflammatory diseases by suppressing inflammation.

In addition, it has been confirmed that the mixed extracts of lemon myrtle, rhubarb, and Angelica chinensis inhibit inflammation in brain tissue. In the present invention, the inflammatory disease may specifically be a brain or neuroinflammatory disease.

The brain or neuroinflammatory disease may be a degenerative brain disease caused by oxidative stress or excessive inflammatory response, and specifically, Alzheimer's disease, Parkinson's disease, Huntington's disease, and amyotrophic lateral sclerosis. (ALS; Amyotrophic Lateral Sclerosis) may be one selected from the group consisting of.

In the present invention, "treatment" means reduction, suppression, sedation or eradication of an infectious disease or inflammatory disease, which is a target disease of the present invention. In the present invention, "prevention" means any action that inhibits or delays the onset of an infectious disease or inflammatory disease, which is the target disease of the present invention, and includes a therapeutic meaning.

The pharmaceutical composition of the present invention can be administered in a pharmaceutically effective amount.

In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease with a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level is subject type and severity, age, sex, disease type, drug activity, drug sensitivity, administration time, route of administration and excretion rate, duration of treatment, factors including drugs used concurrently, and other factors well known in the medical field. The composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents. And it can be single or multiple administrations. It is important to administer the amount that can obtain the maximum effect with the minimum amount without side effects in consideration of all the above factors, and can be easily determined by those skilled in the art.

The pharmaceutical composition of the present invention is not particularly limited as long as it is a subject for the purpose of treating or preventing an infectious disease or an inflammatory disease, and any one can be applied. For example, non-human animals such as monkeys, dogs, cats, rabbits, guinea pigs, rats, mice, cows, sheep, pigs, goats, and the like, humans, birds, and fish may be used, and the pharmaceutical composition may be administered parenterally or subcutaneously. , can be administered intraperitoneally, intrapulmonaryly and intranasally, and for local treatment, if necessary, by any suitable method including intralesional administration. The preferred dosage of the pharmaceutical composition of the present invention varies depending on the condition and body weight of the subject, the severity of the disease, the drug type, the route of administration and the period, but can be appropriately selected by those skilled in the art. For example, it may be administered by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine intrathecal or intracerebrovascular injection, but is not limited thereto.

The pharmaceutical composition is any one selected from the group consisting of tablets, pills, powders, granules, capsules, suspensions, internal solutions, emulsions, syrups, sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried agents, and suppositories. It may have a formulation, and may be of various oral or parenteral formulations. When formulated, it is prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc. These solid preparations contain at least one excipient such as starch, calcium carbonate, sucrose or lactose, gelatin It can be prepared by mixing etc. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral administration include suspensions, internal solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, aromatics, and preservatives may be included. there is. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried formulations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents. As a base for the suppository, witepsol, macrogol, tween 61, cacao butter, laurin paper, glycerogelatin, and the like may be used.

A suitable total daily amount can be determined by a treating physician within the scope of sound medical judgment, and is generally 0.001 to 1000 mg/kg, preferably 0.05 to 200 mg/kg, more preferably 0.1 to 100 mg/kg. The amount of kg can be divided and administered once a day to several times.

In another aspect, the present invention relates to a quasi-drug composition for antioxidant, anti-inflammatory, or antibacterial use comprising a mixed extract of lemon myrtle, rhubarb, and angelica quasi as an active ingredient.

In the present invention, descriptions of lemon myrtle, mulberry, angelica, mixed extract, antioxidant, anti-inflammatory, and antibacterial properties are as described above.

In the present invention, "quasi-drug" refers to items that have a milder action than pharmaceuticals among items used for the purpose of diagnosing, treating, improving, mitigating, treating or preventing diseases of humans or animals. For example, according to the Pharmaceutical Affairs Act, quasi-drugs are Textile/rubber products used for the treatment or prevention of human/animal diseases, non-tools or machines that have little or no direct action on the human body, and similar products that do not act directly on the human body, excluding items used for medicinal purposes; This includes disinfectants and insecticides to prevent it.

The type or formulation of the quasi-drug composition of the present invention is not particularly limited, but is preferably a disinfectant cleanser, shower foam, gargreen, wet tissue, detergent soap, hand wash, humidifier filler, mask, ointment, or filter filler.

When the composition of the present invention is included in a quasi-drug for antioxidant, anti-inflammatory or antibacterial purposes, the composition may be used as it is or used together with other quasi-drug ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined depending on the purpose of use, and the quasi-drug composition according to the present invention may contain the mixed extract of lemon myrtle, chinquapin, and angelica quasi in an amount of 0.01 to 20% by weight based on the total weight of the composition.

In another aspect, the present invention relates to a food composition for anti-pollution comprising a mixture of extracts of lemon myrtle, rhubarb, and Angelica quai as an active ingredient.

In the present invention, descriptions of lemon myrtle, rhubarb, angelica, mixed extract, and food are as described above.

In the present invention, “anti-pollution” means suppressing oxidative stress or inflammation induced by yellow dust or fine dust.

In the above-described examples of the present invention, it was confirmed that the mixed extract of lemon myrtle, rhubarb, and Angelica quai had excellent DPPH radical scavenging activity, and it was confirmed that the antioxidant effect was excellent by suppressing the production of lipid peroxide induced by LPS in mice. , It is possible to protect cells against yellow dust or fine dust by inhibiting or reducing oxidative stress induced in cells by yellow dust or fine dust.

In addition, when inflammation was induced in the brain tissue with LPS in mice to which the mixed extracts of lemon myrtle, porphyria and angelica were orally administered, astrocytes (GFAP-positive cells) and microglial cells, which are inflammation-related cells, were compared to the control group administered only with LPS. (Iba-1 positive cells) was confirmed to be inhibited, the mixed extract of lemon myrtle, lactobacillus and angelica guinea according to the present invention can protect cells by inhibiting or preventing inflammation induced by fine dust.

In addition, it was confirmed that oral administration of the mixed extracts of lemon myrtle, pomegranate, and Angelica quai suppressed inflammation in brain tissue, and the anti-pollution of the present invention was specifically applied to nerves or brain cells caused by inflammation induced by fine dust. It may be to protect cells from damage.

Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

1. Preparation of Mixed Extracts of Lemon Myrtle, McMoondong and Dong Quai

After mixing dried lemon myrtle leaves, coriander, and Angelica gigas in the weight ratio of Table 1, distilled water as a solvent was mixed and extracted for 3 hours using a pressurized extractor at 100 ° C, followed by filtration, concentration, freezing at -75 ° C deepfreezer, and then freeze-drying. was used as a sample.

As a result of cytotoxicity analysis on the prepared mixed extract, Example 1 had no cytotoxicity, but Comparative Example 1 and Comparative Example 2 showed high cytotoxicity, so further experiments were not performed.

sample name mixed weight ratio lemon myrtle Mcmun-dong angelica Example 1 (LMD) 2 5 3 Comparative Example 1 2 One 3 Comparative Example 2 2 10 3

2. Analysis of antibacterial effect

1) Microbial culture

Staphylococcus aureus , Staphylococcus epidermidis , Listeria monocytogenes , and Escherichia coli were acquired from the Microbial Resource Center (Daejeon, Korea) of the Korea Research Institute of Bioscience and Biotechnology. Microbial culture was performed using Tryptic soy broth (TSB, Difco Co.) or Tryptic soy broth agar (TSA, Difco Co.) in a 37° C. incubator for 24 to 39 hours depending on the strain. It was cultured and used for antibacterial activity test.

2) Measurement of antibacterial effect

Antibacterial activity is measured by the paper disc method. After taking 1 platinum amount of each strain cultured on the plate medium and culturing in 10 ml of liquid medium for 18 to 48 hours, 0.1 ml was inoculated into 10 ml of liquid medium again and cultured for 3 to 6 hours.

After uniformly smearing the strain on the plate medium with a sterile cotton swab, a sterilized 8 mm filter paper disc is placed on the solid plate medium. The sample was absorbed into a filter paper disc for each concentration and incubated at 37° C. or 25° C. for 18 to 48 hours, and the diameter of the clear zone (mm) around the disc was measured.

3) Evaluation of the antibacterial effect of mixed extracts (LMD) of lemon myrtle, licorice root, and Angelica quai

A mixed extract (Example 1, LMD) of lemon myrtle, rhubarb, and Angelica quai was diluted to concentrations of 0%, 1%, 3%, 5%, and 10% and used in the experiment, and the analysis results are shown in FIG. 1. In FIG. 1, the top center disc is a 0% sample, and 1%, 3%, 5%, and 10% are arranged in a clockwise direction.

The mixed extracts of lemon myrtle, pulmona sinensis and dong quai clearly showed clear zones even when diluted samples were treated with Staphylococcus aureus and Staphylococcus epidermidis at a concentration of 1%. It was confirmed that the mixed extract of Staphylococcus aureus and Staphylococcus epidermidis showed very excellent antibacterial activity even at a low concentration (1% concentration) (FIG. 1).

Mixed extracts of lemon myrtle, rhubarb, and Angelica gigas showed clear zones at concentrations above 5% against Listeria monocytogenes , showing some antibacterial activity against Listeria monocytogenes. . In Listeria monocytogenes, the formation of a band as if the control (control, 0%) showed an antibacterial effect is presumed to have an antibacterial effect as distilled water temporarily spreads when dispensed on a paper disc did When looking at the results of the control group in other strains, it was determined that the control group (0%) did not exhibit antibacterial activity (FIG. 1).

On the other hand, it was confirmed that Escherichia coli did not show an antibacterial effect against Escherichia coli because a clear zone did not appear even when the mixed extract of lemon myrtle, coriander, and Angelica quai was treated at a concentration of 10%.

3. In vitro ( In vitro ) Antioxidant effect analysis

1) DPPH radical scavenging activity assay

Mixed extracts of lemon myrtle, rhubarb, and Angelica quai (Example 1, LMD) were dissolved in distilled water at concentrations of 50, 100, 500, and 1000 μg/ml and used in the experiment. A DPPH solution was prepared by mixing 3 ml of ethanol and 0.5 ml of DMSO with 1.5 ml of a solution of 3 mg of DPPH dissolved in 15 ml of ethanol.

DPPH radical scavenging activity was obtained by measuring the absorbance at 517 nm using a spectrophotometer after mixing 50 μl of the sample and the DPPH solution and reacting at room temperature for 10 minutes. The DPPH radical scavenging activity was analyzed by calculating the electron donating ability (EDA) as shown in Equation 1.

A: absorbance of sample, B: absorbance of control

As a result of analyzing the DPPH scavenging activity in a test tube to measure the antioxidant effect of the mixed extract of lemon myrtle, rhubarb, and Angelica quai (LMD), the mixed extract of lemon myrtle, rhubarb, and angelica quai (LMD) showed a concentration-dependent DPPH The radical scavenging ability was increased, and the EC ₅₀ (Half maximal effective concentration) was 116.9 μM (FIG. 2).

4. In animal models ( In-vivo ) Analysis of antioxidant and anti-inflammatory effects

1) Preparation of experimental animals

Male ICR mice (6 weeks old) were purchased from Samtaco Bio and acclimatized for one week before use. The animals were reared in an individual breeding device with a temperature of 22 ± 1 °C and a humidity of 50 ± 10% with day/night cycles set at 12-hour cycles, and water and food were provided on an autonomous diet. The experimental groups were a non-administration control group (sham) to which no LPS and sample samples were administered, an LPS control group (control), and a low-dose group (100 mg/kg), a high-dose group (1000 mg/kg) of a mixed extract (LMD) of lemon myrtle, rhubarb, and angelica quai, and a positive control group (ferulic acid).

All animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) of Dong-A University (DIACUC-approval-20-18).

2) Drug (sample) administration and brain sample collection

The administration doses of the mixed extract (LMD) of lemon myrtle, rhubarb, and Angelica quai were set at a low dose (100 mg/kg) and a high dose (1000 mg/kg). Ferulic acid used as a positive control was administered at the dose (20 mg/kg) used in the study.

LPS was dissolved in PBS and administered at a dose of 1 μg/3 μl to the third ventricle. Each sample was dissolved in 10% tween 80 solution and orally administered 3 days before LPS administration.

Samples were orally administered for 7 days after LPS administration, and brain tissues of experimental animals were collected after 7 days. Half of the brain tissue was ground for western blot and ELISA analysis, and the other half was post-fixed for tissue immunostaining.

3) Antioxidant effect evaluation: Lipid peroxidation analysis (MDA measurement)

To analyze the antioxidant effect of the mixed extracts of lemon myrtle, rhubarb, and angelica quai, brain inflammation was induced in mice with LPS, and lipids, an indicator of oxidative damage, were administered with the mixed extracts of lemon myrtle, rhubarb, and angelica quai (LMD). Changes in the production of peroxide (MDA) were analyzed.

To measure changes in lipid peroxide, rat hippocampal tissue was homogenized at 4° C. using phosphate buffered saline (PBS). Then, the supernatant was used by centrifugation at 10,000 rpm for 5 minutes. Samples were treated with 0.15 M potassium chloride and 500 μl of each homogenized tissue, and 100 μl of 20 mM ferric chloride was added. Then, after reacting for 30 minutes in a 37° C. incubator, 500 μl of 0.25 N cold-HCl containing 15% TCA, 0.38% TBA and 0.5% BHT was added. After heating at 90 ° C. for 60 minutes, centrifugation was performed at 5,000 rpm and 4 ° C. for 5 minutes to measure thiobarbituric acid reactive substances (TBARS) in the supernatant at an absorbance of 532 nm.

As a result of the analysis, LPS increased the amount of MDA produced in the brains of experimental animals by 170%. This means that oxidative damage was induced by LPS. It was confirmed that the high-dose administration (1000 mg/kg) of the mixed extracts (LMD) of lemon myrtle, rhubarb, and angelica quasi statistically significantly reduced the amount of MDA produced by LPS.

In addition, it was confirmed that ferulic acid (FA) used as a positive control also inhibited MDA production by LPS (FIG. 3).

4) Evaluation of anti-inflammatory effect: tissue immunostaining analysis of inflammatory cells

To analyze the anti-inflammatory effect of the mixed extract of lemon myrtle, rhubarb, and Angelica quai, brain inflammation was induced in mice with LPS, and then the mixed extract of lemon myrtle, rhubarb, and angelica quai was orally administered (LMD) to the brain tissue. The degree of increase in microglia and astrocytes, which are inflammation-related cells, was analyzed. Specifically, microglia using the Iba-1 antibody and astrocytes using the GFAP antibody were subjected to tissue immunostaining to analyze the degree of increase in each cell.

The hippocampus of the fixed brain tissue was picked, washed three times with PBS, and then treated with hydrogen peroxide to remove endogenous peroxidase. Thereafter, the primary antibody rabbit anti-Iba-1 (diluted 1:1000) or rabbit anti-GFAP (diluted 1:1000) was reacted overnight. As the secondary antibody, biotin and anti-rabbit (1:200 dilution) were used, followed by ABC reaction, followed by color development using DAB for 3 minutes.

Three washes were done with PBS between each procedure. After completion of the staining reaction, the cover slide with the hippocampal tissue was covered with a mounting solution, dried, and stored. The brain tissue was mounted on a gelatin-coated slide, subjected to a process of 70 to 100% ethanol and xylene, and covered with a cover slide to store the tissue. Stained hippocampal slices were observed using an optical microscope (Olympus Microscope System BX51; Olympus, Tokyo, Japan). The area of each antibody-positive region was expressed as a ratio to the total area of the hippocampus.

As a result of the analysis, LPS induced an increase in GFAP-positive cells, a marker for astrocytes, and Iba-1-positive cells, a marker for microglial cells, in the brains of experimental animals. Oral administration of mixed extracts (LMD) of lemon myrtle, rhubarb, and Angelica quai significantly reduced the increase in astrocytes (GFAP-positive cells) and microglial cells (Iba-1-positive cells) induced by LPS.

The positive control group (ferulic acid, FA) also inhibited the increase of astrocytes by LPS, but the increase of microglial cells only showed a decreasing trend and did not show statistical significance (FIG. 4).

In other words, it was confirmed that oral administration of the mixed extract (LMD) of lemon myrtle, rhubarb, and Angelica quai exhibited an anti-inflammatory effect in animal models.

Claims (10)

Contains as an active ingredient a mixed extract obtained by hot water extraction by mixing lemon myrtle, coriander, and angelica in a weight ratio of 1 to 3: 4 to 6: 2 to 4.
A food composition for antioxidant, anti-inflammatory and antibacterial purposes. delete delete According to claim 1,
The antibacterial is to inhibit the growth of one or more microorganisms selected from the group consisting of Staphylococcus aureus , Staphylococcus epidermidis and Listeria monocytogenes , antioxidant, anti-inflammatory and Antibacterial food composition. Contains as an active ingredient a mixed extract obtained by hot water extraction by mixing lemon myrtle, coriander, and angelica in a weight ratio of 1 to 3: 4 to 6: 2 to 4.
Antioxidant, anti-inflammatory and antibacterial cosmetic composition. Contains as an active ingredient a mixed extract obtained by hot water extraction by mixing lemon myrtle, coriander, and angelica in a weight ratio of 1 to 3: 4 to 6: 2 to 4.
A pharmaceutical composition for preventing or treating inflammatory diseases. According to claim 6,
Wherein the inflammatory disease is a brain or neuroinflammatory disease, the pharmaceutical composition Contains as an active ingredient a mixed extract obtained by hot water extraction by mixing lemon myrtle, coriander, and angelica in a weight ratio of 1 to 3: 4 to 6: 2 to 4.
Antioxidant, anti-inflammatory and antibacterial quasi-drug composition. delete delete

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