KR102539450B1 - Method for manufacturing atopic dermatitis-improving cosmetic composition containing tarantula component - Google Patents

Abstract

The present invention provides an atopic dermatitis-alleviating cosmetic composition containing a tarantula component and an Angelica keiskei extract.

Description

Method for manufacturing atopic dermatitis-improving cosmetic composition containing tarantula component}

The present invention relates to a method for preparing a cosmetic composition for improving atopy containing a tarantula component, and relates to a cosmetic composition for improving atopy containing a peptide component obtained from tarantula and a method for manufacturing the same.

In general, atopic skin occurs mainly in infancy and is also called fetal fever. It is mainly caused by a decrease in the content of ceramides in the stratum corneum. As the skin barrier function deteriorates, irritating substances penetrate into the stratum corneum and cause irritation. indicate When such atopic skin is severely scratched, eczema and keloid phenomena are accompanied, and severe keratinization occurs. In particular, these atopic skin diseases are not easily treated even as they grow, causing significant difficulties in social life or activities in adults, and eventually causing serious social phenomena such as social phobia.

So far, four types of atopic skin have been identified. The first is due to genetic causes. Due to the lack of gamma-linoleic acid (GLA), the skin barrier cannot be hardened, so moisture on the skin surface is easily lost, and allergies easily occur due to external stimuli. Second, it is caused by environmental factors. As the residential environment of modern people is mainly apartment life, and the living environment changes to a very dry and closed state, atopic skin is increasing, which lowers the water retention capacity and improves the skin protection function. Allergy occurs due to a sensitive response even to small stimuli. Thirdly, it is caused by active oxygen, and lipid peroxide is generated by active oxygen, which causes atopic skin. Fourth, it is known to be caused by infection with various harmful bacteria, viruses, fungi, etc., and it is believed that these causes occur in a complex relationship.

In order to improve such atopic skin, in principle, clean the skin to remove substances that can act as antigens, strengthen the skin barrier function, and supply ceramide, an intercellular lipid component that is insufficient to protect the skin from drying skin, to stimulate the stratum corneum. I have been using methods to avoid physical stimuli so as not to give them. Therefore, in order to improve atopic skin, ingredients such as known ceramides, linoleic acid plants or mineral oils, steroid agents such as hydrocortisone, or specific plant extracts are included in the formulation of general emulsifying products, or It is made into a liposome using lecithin, a phospholipid component, and supplied to the stratum corneum to create a moisturizing effect and an oil film to prevent water loss, and to prevent bacterial contamination. Skin external preparations or cosmetics with enhanced components of anti-inflammatory function have been mainly used.

However, the conventional methods as described above, which have been known so far, have achieved some success in preventing and treating atopic skin, but still have many problems in terms of effectiveness or skin stability. For example, it has been reported that the use of steroids used in external preparations for atopic skin can cause adverse effects such as epidermal growth inhibition or side effects, and urea proxide has concerns about skin irritation and other contact dermatitis, and antihistamines. It is known that antibiotics such as antibiotics have the possibility of developing bacterial resistance and photosensitization. In addition, in the case of cosmetics using some drug extracts, there is a problem in that there is a problem in that there is a problem in that there is a limitation in use because there is a case of allergic hypersensitivity reaction.

On the other hand, the GsMTx4 peptide extracted from the poison secreted by the Chilean Rose Tarantula (scientific name: Grammostula spatulata) spider has 34 amino acids (molecular weight: about 4 kDa) and is about 50% less effective than other similar peptide toxins. It is known to have homology (Oswald RE et al., J Biol Chem. 277(37):34443-50, 2002), and inhibits SAC in addition to the GsMTx4 peptide in Grammostola spatulata spider venom. Other peptides with activity (Sachs, F., unpublished observations) and GsMTx-1 (US Pat. No. 5756663) have been isolated (Thomas M. Suchyna, Janice H. Johnson, Katherine Hamer, Joseph F. Leykam, Douglas A. Gage, Henry F. Clemo, Clive M. Baumgarten, and Frederick Sachs, The Journal of General Phyiology, Volume 115, Number 5, 583-598, May 1, 2000).

In addition, Korean Patent Registration No. 10-0761792 discloses that the GsMTx4 peptide extracted from Chilean rose tarantula spider venom is a pain-inducing substance and suppresses the MSC activity of nociceptive neurons activated by mechanical stimulation in a model showing hypersensitivity to stimulation. It has been found to function as an analgesic. However, application as a composition for improving atopy is insignificant, and more research is needed.

The present invention has been made to solve the above problems, and an object of the present invention is to provide a cosmetic composition effective for improving atopy by combining a tarantula component and an extract of Sinensis.

The object of the present invention is not limited to the tasks mentioned above, and other tasks not mentioned will be clearly understood by those skilled in the art from the following description.

In order to achieve the above object, the present invention

Provided is a cosmetic composition for improving atopy containing a tarantula component and an extract of Sintacola.

In addition, the cosmetic composition for improving atopy,

1-5 parts by weight of Grammostula spatulata mechanotoxin 4 (GsMTx4) peptide, 0.3-3 parts by weight of fresh grass leaf extract, 0.3-3 parts by weight of clover clover leaf extract, 0.3-3 parts by weight of honeysuckle leaf extract 3 parts by weight, clover flower extract 0.3-3 parts by weight, peony flower extract 0.3-3 parts by weight, evening primrose extract 0.3-3 parts by weight, sea daffodil oil 0.3-3 parts by weight, lavender oil 0.3-3 parts by weight, derived from Soja Exosome 0.3-3 parts by weight, niacinamide 0.5-4 parts by weight, arginine 0.3-3 parts by weight, adenosine 0.3-3 parts by weight, diethylaminohydroxybenzoylhexylbenzoate 0.3-3 parts by weight, triethylhexanoin 0.3 -3 parts by weight, squalane 3-7 parts by weight, butylene glycol 2-6 parts by weight, propylene glycol 0.05-0.2 parts by weight, polyglyceryl-6 stearate 0.05-0.2 parts by weight, stearyl alcohol 0.05- 0.2 parts by weight, isoquercitrin 0.05-0.2 parts by weight, hyaluronic acid 0.5-4 parts by weight, sodium hyaluronate 0.05-0.2 parts by weight, limonene 0.3-0.7 parts by weight, linalool 0.3-3 parts by weight, and It is characterized in that it comprises 67-71 parts by weight of broccoli water.

In addition, the cosmetic composition for improving atopy,

2-4 parts by weight of Grammostula spatulata mechanotoxin 4 (GsMTx4) peptide, 0.5-1.5 parts by weight of fresh grass leaf extract, 0.5-1.5 parts by weight of clover leaf extract, 0.5-1.5 parts by weight of honeysuckle leaf extract 1.5 parts by weight, clover flower extract 0.5-1.5 parts by weight, peony flower extract 0.5-1.5 parts by weight, evening primrose extract 0.5-1.5 parts by weight, sea daffodil oil 0.5-1.5 parts by weight, lavender oil 0.5-1.5 parts by weight, Soja Exosome 0.5-1.5 parts by weight, niacinamide 1-3 parts by weight, arginine 0.5-1.5 parts by weight, adenosine 0.5-1.5 parts by weight, diethylaminohydroxybenzoylhexylbenzoate 0.5-1.5 parts by weight, triethylhexanoin 0.5 parts by weight -1.5 parts by weight, squalane 4-6 parts by weight, butylene glycol 3-5 parts by weight, propylene glycol 0.08-0.12 parts by weight, polyglyceryl-6 stearate 0.08-0.12 parts by weight, stearyl alcohol 0.08- 0.12 parts by weight, isoquercitrin 0.08-0.12 parts by weight, hyaluronic acid 1-3 parts by weight, sodium hyaluronate 0.08-0.12 parts by weight, limonene 0.4-0.6 parts by weight, linalool 0.5-1.5 parts by weight and It is characterized in that it comprises 68-70 parts by weight of broccoli water.

In addition, the present invention

Preparing fresh herb leaf powder, clover clover leaf powder, and honeysuckle leaf powder by washing, freeze-drying, and pulverizing fresh herb leaves, clover clover leaf and honeysuckle leaf; Prepared fresh herb leaf powder, clover leaf powder and honeysuckle leaf powder were mixed in a weight ratio of 1: 1: 1, and a mixed solvent in which ethanol and hexane were mixed in a volume ratio of 1: 1 and a weight ratio of 1: 9 preparing an extract by mixing and extracting at a temperature of 28-32 ° C for 22-26 hours; and filtering the extract with a filter paper, concentrating the filtered filtrate under reduced pressure and freeze-drying the filtrate at minus 50 ° C.

Preparing clover flower powder, peony flower powder, and evening primrose powder by washing, freeze-drying, and pulverizing clover flower, peony flower, and evening primrose; 20 g of the prepared mixed powder of clover flower powder, peony flower powder, and evening primrose powder mixed in a weight ratio of 1:1:1 was mixed with 200 ml of water, followed by primary hot water at 80°C for 8 hours while stirring. performing an extraction; After the first hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a first mixed extract; After the first hot water extraction, the remaining solids were filtered with filter paper, and the remaining solids were mixed with water so that the mixing ratio of the solids and water was 0.1 g/ml, and the second hot water extraction was performed at a temperature of 120 ° C for 12 hours while stirring. doing; After secondary hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a secondary mixed extract; After the second hot water extraction, filter with filter paper, and mix the remaining solids with water again so that the mixing ratio of solids and water is 0.1 g/ml, and [BMIm][TFSI](1-Butyl-3-methylimidazolium After adding 1-2 g of trifluoromethylsulfonyl)imide), performing a third hot water extraction at a temperature of 100 ° C. for 8 hours while stirring; After tertiary hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a tertiary mixed extract; And mixing the first mixed extract, the second mixed extract and the tertiary mixed extract; preparing a bush clover flower extract, a peony flower extract and an evening primrose extract containing;

Preparing sea daffodil powder and lavender powder by crushing sea daffodil and lavender; preparing an extract by mixing a mixture of daffodil powder and lavender powder in a weight ratio of 1:1 with hexane and stirring at a temperature of 33-37 ° C. for 2-4 days; Preparing sea daffodil oil and lavender oil comprising; filtering and concentrating the extract under reduced pressure, centrifuging to remove foreign substances, and distilling under reduced pressure;

washing the device and pre-treating it at a temperature of 28-32° C. and a pressure of 290-310 MPa for 1-5 minutes; Preparing a device juice by extracting the pretreated device; Obtaining a supernatant containing exosomes by centrifuging the juice from the cow; preparing a lyophilisate by freeze-drying the supernatant containing the exosomes; forming an aqueous two-phase system using polyethylene glycol/dextran in the lyophilisate; and obtaining an exosome-enriched lower layer in the aqueous two-phase system; preparing a device-derived exosome comprising;

1-5 parts by weight of Grammostula spatulata mechanotoxin 4 (GsMTx4) peptide, 1-5 parts by weight of the above-prepared cinnamon leaf extract, clover clover leaf extract and honeysuckle leaf extract, 1-5 parts by weight of Hsagi flower extract, peony flower extract and evening primrose extract prepared above, 0.3-3 parts by weight of the device-derived exosome prepared above, 0.5-4 parts by weight of niacinamide, 0.3-3 parts by weight of arginine, 0.3 parts by weight of adenosine -3 parts by weight, diethylaminohydroxybenzoylhexylbenzoate 0.3-3 parts by weight, triethylhexanoin 0.3-3 parts by weight, stearyl alcohol 0.05-0.2 parts by weight, isoquercitrin 0.05-0.2 parts by weight, hyaluronic acid 0.5-4 parts by weight of rhonic acid, 0.05-0.2 parts by weight of sodium hyaluronate, 0.3-0.7 parts by weight of limonene, 0.3-3 parts by weight of linalool and 47-51 parts by weight of broccoli water were mixed, and Stirring for 28-32 minutes at a temperature to prepare a first mixed composition;

0.5-4 parts by weight of daffodil oil and lavender oil prepared above, 3-7 parts by weight of squalane, 2-6 parts by weight of butylene glycol, 0.05-0.2 parts by weight of propylene glycol, polyglyceryl-6 stearate Preparing a second mixed composition by mixing 0.05-0.2 parts by weight and 18-22 parts by weight of broccoli water and stirring at a temperature of 38-42 ° C. for 58-62 minutes; and

It provides a method for producing a cosmetic composition for improving atopic dermatitis, comprising: mixing the first mixed composition and the second mixed composition and stirring for 18-22 minutes at a temperature of 48-52 ° C.

In addition, the first mixed composition is characterized in that it comprises 0.3-3 parts by weight of rice bran fermented extract.

The cosmetic composition for improving atopy according to the present invention is effective in improving atopy by combining a tarantula component and an extract of cinnamon plant.

1 is a flow chart showing a method for producing a cosmetic composition for improving atopy according to the present invention.

Hereinafter, various embodiments will be described in more detail with reference to the accompanying drawings. The embodiments described in this specification may be modified in various ways. Certain embodiments may be depicted in the drawings and described in detail in the detailed description. However, specific embodiments disclosed in the accompanying drawings are only intended to facilitate understanding of various embodiments. Therefore, the technical idea is not limited by the specific embodiments disclosed in the accompanying drawings, and it should be understood to include all equivalents or substitutes included in the spirit and technical scope of the invention.

Terms including ordinal numbers such as primary, secondary, first, and second may be used to describe various elements, but these elements are not limited by the above-mentioned terms. The terminology described above is only used for the purpose of distinguishing one component from another.

In this specification, terms such as 'comprise' or 'having' are intended to designate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, but one or more other features It should be understood that the presence or addition of numbers, steps, operations, components, parts, or combinations thereof is not precluded. It is understood that when a component is referred to as being 'connected' or 'connected' to another component, it may be directly connected or connected to the other component, but other components may exist in the middle. It should be. On the other hand, when a component is referred to as being 'directly connected' or 'directly connected' to another component, it should be understood that no other component exists in the middle.

In addition, in describing the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be abbreviated or omitted.

the present invention

Provided is a cosmetic composition for improving atopy containing a tarantula component and an extract of Sintacola.

Hereinafter, the cosmetic composition for improving atopy according to the present invention will be described in detail.

The cosmetic composition for improving atopy according to the present invention is effective in improving atopy by combining a tarantula component and an extract of cinnamon plant.

The cosmetic composition for improving atopy includes 1-5 parts by weight of Grammostula spatulata mechanotoxin 4 (GsMTx4) peptide, 0.3-3 parts by weight of fresh grass leaf extract, and 0.3-3 parts by weight of clover leaf extract. , honeysuckle leaf extract 0.3-3 parts by weight, clover flower extract 0.3-3 parts by weight, peony flower extract 0.3-3 parts by weight, evening primrose extract 0.3-3 parts by weight, sea daffodil oil 0.3-3 parts by weight, lavender oil 0.3 parts by weight -3 parts by weight, soma-derived exosome 0.3-3 parts by weight, niacinamide 0.5-4 parts by weight, arginine 0.3-3 parts by weight, adenosine 0.3-3 parts by weight, diethylaminohydroxybenzoylhexylbenzoate 0.3-3 parts by weight 0.3-3 parts by weight of triethylhexanoin, 3-7 parts by weight of squalane, 2-6 parts by weight of butylene glycol, 0.05-0.2 parts by weight of propylene glycol, 0.05-0.2 parts by weight of polyglyceryl-6stearate stearyl alcohol 0.05-0.2 parts by weight, isoquercitrin 0.05-0.2 parts by weight, hyaluronic acid 0.5-4 parts by weight, sodium hyaluronate 0.05-0.2 parts by weight, limonene 0.3-0.7 parts by weight, Li It is preferable to include 0.3-3 parts by weight of nalrul and 67-71 parts by weight of broccoli water, and 2-4 parts by weight of Grammostula spatulata mechanotoxin 4 (GsMTx4) peptide, 0.5- 1.5 parts by weight, bush clover leaf extract 0.5-1.5 parts by weight, honeysuckle leaf extract 0.5-1.5 parts by weight, bush clover flower extract 0.5-1.5 parts by weight, peony flower extract 0.5-1.5 parts by weight, evening primrose extract 0.5-1.5 parts by weight , sea daffodil oil 0.5-1.5 parts by weight, lavender oil 0.5-1.5 parts by weight, elemental exosomes 0.5-1.5 parts by weight, niacinamide 1-3 parts by weight, arginine 0.5-1.5 parts by weight, adenosine 0.5-1.5 parts by weight, 0.5-1.5 parts by weight of diethylaminohydroxybenzoylhexylbenzoate, 0.5-1.5 parts by weight of triethylhexanoin, 4-6 parts by weight of squalane, 3-5 parts by weight of butylene glycol, 0.08-0.12 parts by weight of propylene glycol 0.08-0.12 parts by weight of polyglyceryl-6 stearate, 0.08-0.12 parts by weight of stearyl alcohol, 0.08-0.12 parts by weight of isoquercitrin, 1-3 parts by weight of hyaluronic acid, 0.08 parts by weight of sodium hyaluronate -0.12 parts by weight, limonene 0.4-0.6 parts by weight, linalool 0.5-1.5 parts by weight and broccoli water 68-70 parts by weight more preferably included.

Grammostula spatulata mechanotoxin 4 (GsMTx4) peptide is a peptide component presented in already known literature (Korean Patent Application No. 2005-0098082), etc., and in the present invention, it is applied to a cosmetic composition for improving atopy so that the effect could be confirmed.

Fresh herbs are effective for high blood pressure, low blood pressure, arteriosclerosis, shoulder pain, anemia, etc., and are also good for tonic, diuretic, skin soothing, anti-inflammatory, antibacterial, and allergy effects, and have the effect of keeping the skin moist by improving blood circulation. there is.

Hagi is a deciduous shrub of the leguminous family that grows commonly in mountains and fields at a height of 2 to 3 m, and flowers in July and August. The bark and stem leaves contain saponin. In particular, the root bark contains alkaloids. The leaves of the clover tree can be ironed and used to treat nephritis, renal failure, renal sclerosis, renal azotemia, etc., and to prevent and treat hypertension and arteriosclerosis. In addition, Hagi leaves contain a lot of alkaloids, flavonoids, and ascorbic acid, and the bark contains tannins.

Honeysuckle (Lonicera Caprifolium, Honeysuckle), also known as honeysuckle or honeysuckle, is mainly distributed in East Asia. It has antipyretic, detoxifying, antibacterial, and anti-inflammatory properties, and has been used as a treatment for inflammatory and infectious diseases. In the present invention, honeysuckle leaves are used.

In the present invention, it is possible to exhibit excellent antioxidant ability and antibacterial effect effective for atopic dermatitis by applying the fresh herb leaf extract, clover clover leaf extract and honeysuckle leaf extract.

Peony (Paeonia lactiflora, Paeonia albiflora) is a dicotyledonous perennial plant belonging to the peony and peony genus, and is cultivated mainly in China. Peony flowers are large in size and have good color, so they are mainly used only for gardening, but recently, various attempts to use peony flowers for skin care purposes have been continued.

Taking a bath with water infused with bush clover flowers has the effect of making you sweat and getting various wastes from the body out of the body. In addition, it has a unique flavor when it is collected and eaten as a tea or powdered.

Evening primrose contains gamma linolenic acid, which our bodies cannot produce. It has excellent efficacy in treating arthritis, and it is also good for treating inflammation by regulating a substance called prostaglandin. It is good for blood circulation and has an anti-inflammatory effect, so it has the effect of suppressing inflammation of the skin.

In the present invention, by applying a peony flower extract, a bush clover flower extract, and an evening primrose extract, it is possible to exhibit excellent antioxidant ability and antibacterial effect effective for atopic dermatitis.

Sea daffodil (Pancratium Maritimum), also called Sea daffodil, is a type of bulbous plant that grows in the Black Sea and Mediterranean coasts of Portugal, Turkey, Israel, and Syria. Sea daffodils are common on beaches and coastal sands. In the present invention, sea daffodil oil is applied to show moist and bright skin due to excellent moisturizing effect and dark spot reduction.

Lavender is one of 25 to 30 plants belonging to the genus Lamiaceae and Lavender, a dicotyledonous plant, and is cultivated to obtain scented oil from flowers and plants and is also planted for ornamental purposes. Fragrance oil is used as a raw material for perfumes and cosmetics, and is also used to treat headaches or nervous stability. The species Lavandula officnalis or Lavandula angustifolia is mainly used in aromatherapy.

In the present invention, sea daffodil oil and lavender oil are applied to increase the soothing effect of atopic dermatitis.

Perillae Semen is the fruit of Perilla frutescens var. Soja (Perillae Semem) refers to the seeds of perilla (lobule) and is used as a sweating, antitussive, stomach, diuretic, sedative and analgesic.

In the present invention, the soma-derived exosome is applied to enhance the soothing effect of atopic dermatitis.

Niacinamide is a complex of water-soluble vitamin B, and is also named nicotinic acid and niacin. Melanocytes, a component that induces discoloration of the skin, are produced by tyrosinase in the basal layer, the lowest layer of the epidermal layer, which is deposited on the surface of the skin from the basal layer. It moves to the nearby stratum corneum and causes pigmentation. Niacinamide can inhibit pigmentation by interfering with this movement, and it can lighten the pigment deposited in the stratum corneum to bring about a skin whitening effect. In addition, niacinamide may contribute to preventing water loss of the skin by increasing the synthesis of intercellular lipids.

Arginine has a soothing effect on atopic dermatitis and improves skin whitening.

Broccoli contains a large amount of beta-carotene, rutin, ascorbic acid, selenium, quercetin, and glutathione, which have antioxidant effects, as well as nutrients such as calcium, vitamin C, and folic acid, so it is used for skin care. In the present invention, by applying broccoli water obtained through broccoli instead of purified water, the skin soothing effect is higher.

In addition, it is more preferable that the cosmetic composition for improving atopy according to the present invention includes a fermented rice bran extract. The fermented rice bran extract is preferably included in an amount of 0.3-3 parts by weight, more preferably 0.5-1.5 parts by weight, based on 100 parts by weight of the total cosmetic composition.

Since rice bran has a strong lipase action and increases the acid value of the fat, it is easy to deteriorate during storage, and it is more severe when the temperature is high and the humidity is high. In order to extract the active ingredients of rice bran, various studies are being conducted on fermentation methods in which rice bran is fermented by microorganisms. Rice bran fermented extract is rich in various amino acids such as aspartic acid, glutamic acid, and serine, and contains a large amount of physiologically active substances in the human body, so it is applied as a functional material that activates the body's metabolism. In the present invention, it helps to improve atopy.

In addition, the cosmetic composition for improving atopy according to the present invention more preferably includes Jindubal extract, rhubarb extract, agar extract, auditoryrrhiza extract and fusiformis extract. The Jindubal extract, rhubarb extract, agar extract, auditory extract, and fusiformis extract are preferably included in an amount of 0.3-3 parts by weight, and more preferably 0.5-1.5 parts by weight, based on 100 parts by weight of the total cosmetic composition.

Chondrus ocellatus is a dark purple frond with a body length of 5-2 cm. The stem branches into two branches several times at a short distance, and a short stem comes out from the button-shaped root, and this stem spreads out in a fan shape as it widens. It grows mainly on bedrock in the lower intertidal zone, where there is a lot of influence from waves, and is distributed all over the coast of Korea.

Rhubarb (Eisenia bicyclis) is an edible seaweed of brown algae that contains a large amount of physiologically active substances such as phlorotannins, and eckol, phlorofucofuroeckol-A, dieckol, and 8,8′-bieckol have been reported as phlorotannins isolated from rhubarb that have antioxidant activity. It is becoming. In addition, it has been reported that rhubarb phlorotannins have various physiological activities such as antibacterial, anti-inflammatory, antihyperlipidemic and antidiabetic.

Agar-agar (Gelidium amansii) is a type of red algae belonging to the family Agaraceae. It is a type of seaweed and refers to seaweed that is mainly used as the main raw material for agar. As a perennial seaweed, after the breeding season in summer, the upper part of the main body melts away and only the lower part remains, and new sprouts grow again in the spring of the following year. It is distributed in the east coast, the south coast and the outer islands of the Yellow Sea, but the ones in the southern coast of the East Sea are of good quality and are produced the most. It grows attached to a rock at a depth of 20-30m in the sea, and lives in a place with a sandy bottom and good communication with seawater.

Codium fragile, green fleece, green sea fingers, stag · sponge seaweed) is an alga belonging to the hearing genus of the hearing family. On the surface of the auditory body, small tube-shaped pockets called 'follicles' are arranged to form the cortex, while the branches extending from the bottom of the follicles are intertwined to form the inner part. At this time, the entire body is made up of one cell without a septum, so it is also called the 'non-cellular system'. The auditory sense is distributed on the coasts of all parts of the world, and is especially well developed in the reefs near the tidal line. There are many types of auditory groups, the important ones being bead hearing, wheat hearing, and speech hearing.

Hijiki is a type of sea horse and belongs to the brown horse group. It is very rich in calcium, iodine, iron and magnesium. It also lives on reefs in Korea, Japan, and China. Hijiki forms large colonies in the lower intertidal zone. The plant is erect with fibrous roots, and the stem is columnar and branches once pinnately, usually growing more than 10 to 60 cm. The small one of the branches is shaped like a club and at first glance it feels like a leaf. It is a brown algae plant produced in the wild and aquaculture in the clean Wando and the southwest sea (Southern Coast area). Hijiki is rich in minerals and dietary fiber, such as calcium, iron, phosphorus, and urea.

Including the Jindubal extract, rhubarb extract, agar-agar extract, hearing extract and fusiformis extract, it helps to improve atopic dermatitis.

In addition, the present invention

Preparing fresh herb leaf powder, clover clover leaf powder, and honeysuckle leaf powder by washing, freeze-drying, and pulverizing fresh herb leaves, clover clover leaf and honeysuckle leaf; Prepared fresh herb leaf powder, clover leaf powder and honeysuckle leaf powder were mixed in a weight ratio of 1: 1: 1, and a mixed solvent in which ethanol and hexane were mixed in a volume ratio of 1: 1 and a weight ratio of 1: 9 preparing an extract by mixing and extracting at a temperature of 28-32 ° C for 22-26 hours; and filtering the extract with a filter paper, concentrating the filtered filtrate under reduced pressure and freeze-drying the filtrate at minus 50 ° C.

Preparing clover flower powder, peony flower powder, and evening primrose powder by washing, freeze-drying, and pulverizing clover flower, peony flower, and evening primrose; 20 g of the prepared mixed powder of clover flower powder, peony flower powder, and evening primrose powder mixed in a weight ratio of 1:1:1 was mixed with 200 ml of water, followed by primary hot water at 80°C for 8 hours while stirring. performing an extraction; After the first hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a first mixed extract; After the first hot water extraction, the remaining solids were filtered with filter paper, and the remaining solids were mixed with water so that the mixing ratio of the solids and water was 0.1 g/ml, and the second hot water extraction was performed at a temperature of 120 ° C for 12 hours while stirring. doing; After secondary hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a secondary mixed extract; After the second hot water extraction, filter with filter paper, and mix the remaining solids with water again so that the mixing ratio of solids and water is 0.1 g/ml, and [BMIm][TFSI](1-Butyl-3-methylimidazolium After adding 1-2 g of trifluoromethylsulfonyl)imide), performing a third hot water extraction at a temperature of 100 ° C. for 8 hours while stirring; After tertiary hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a tertiary mixed extract; And mixing the first mixed extract, the second mixed extract and the tertiary mixed extract; preparing a bush clover flower extract, a peony flower extract and an evening primrose extract containing;

Preparing sea daffodil powder and lavender powder by crushing sea daffodil and lavender; preparing an extract by mixing a mixture of daffodil powder and lavender powder in a weight ratio of 1:1 with hexane and stirring at a temperature of 33-37 ° C. for 2-4 days; Preparing sea daffodil oil and lavender oil comprising; filtering and concentrating the extract under reduced pressure, centrifuging to remove foreign substances, and distilling under reduced pressure;

washing the device and pre-treating it at a temperature of 28-32° C. and a pressure of 290-310 MPa for 1-5 minutes; Preparing a device juice by extracting the pretreated device; Obtaining a supernatant containing exosomes by centrifuging the juice from the cow; preparing a lyophilisate by freeze-drying the supernatant containing the exosomes; forming an aqueous two-phase system using polyethylene glycol/dextran in the lyophilisate; and obtaining an exosome-enriched lower layer in the aqueous two-phase system; preparing a device-derived exosome comprising;

1-5 parts by weight of Grammostula spatulata mechanotoxin 4 (GsMTx4) peptide, 1-5 parts by weight of the above-prepared cinnamon leaf extract, clover clover leaf extract and honeysuckle leaf extract, 1-5 parts by weight of Hsagi flower extract, peony flower extract and evening primrose extract prepared above, 0.3-3 parts by weight of the device-derived exosome prepared above, 0.5-4 parts by weight of niacinamide, 0.3-3 parts by weight of arginine, 0.3 parts by weight of adenosine -3 parts by weight, diethylaminohydroxybenzoylhexylbenzoate 0.3-3 parts by weight, triethylhexanoin 0.3-3 parts by weight, stearyl alcohol 0.05-0.2 parts by weight, isoquercitrin 0.05-0.2 parts by weight, hyaluronic acid 0.5-4 parts by weight of rhonic acid, 0.05-0.2 parts by weight of sodium hyaluronate, 0.3-0.7 parts by weight of limonene, 0.3-3 parts by weight of linalool and 47-51 parts by weight of broccoli water were mixed, and Stirring for 28-32 minutes at a temperature to prepare a first mixed composition;

0.5-4 parts by weight of daffodil oil and lavender oil prepared above, 3-7 parts by weight of squalane, 2-6 parts by weight of butylene glycol, 0.05-0.2 parts by weight of propylene glycol, polyglyceryl-6 stearate Preparing a second mixed composition by mixing 0.05-0.2 parts by weight and 18-22 parts by weight of broccoli water and stirring at a temperature of 38-42 ° C. for 58-62 minutes; and

It provides a method for producing a cosmetic composition for improving atopic dermatitis, comprising: mixing the first mixed composition and the second mixed composition and stirring for 18-22 minutes at a temperature of 48-52 ° C.

At this time, the manufacturing method of the cosmetic composition for improving atopy according to the present invention is shown in a flowchart in Figure 1, and the manufacturing method of the cosmetic composition for improving atopy according to the present invention according to the present invention will be described in detail with reference to FIG.

First, the method for preparing a cosmetic composition for improving atopy according to the present invention includes the steps of preparing an extract of fresh grass leaves, an extract of Hsalt japonica leaves, and an extract of honeysuckle leaves. In the step of preparing the fresh herb leaf extract, clover clover leaf extract and honeysuckle leaf extract, the fresh herb leaf, clover clover leaf and honeysuckle leaf are washed, freeze-dried, and then pulverized to obtain fresh herb leaf powder, clover clover leaf powder and honeysuckle leaf. preparing the powder; Prepared fresh herb leaf powder, clover leaf powder and honeysuckle leaf powder were mixed in a weight ratio of 1: 1: 1, and a mixed solvent in which ethanol and hexane were mixed in a volume ratio of 1: 1 and a weight ratio of 1: 9 preparing an extract by mixing and extracting at a temperature of 28-32 ° C for 22-26 hours; and filtering the extract with a filter paper, and concentrating and lyophilizing the filtered filtrate at -50 °C under reduced pressure.

Antioxidants can be easily extracted from the fresh herb leaf extract, clover clover leaf extract, and honeysuckle leaf extract by an extraction method using a mixed solvent in which ethanol and hexane are mixed in a volume ratio of 1:1.

Next, the method for preparing the cosmetic composition for improving atopy according to the present invention includes preparing a clover flower extract, a peony flower extract, and an evening primrose extract. The step of preparing the clover clover flower extract, peony flower extract, and evening primrose extract includes washing, freeze-drying, and pulverizing clover clover flower powder, peony flower powder, and evening primrose flower powder, peony flower powder, and evening primrose flower powder; 20 g of the prepared mixed powder of clover flower powder, peony flower powder, and evening primrose powder mixed in a weight ratio of 1:1:1 was mixed with 200 ml of water, followed by primary hot water at 80°C for 8 hours while stirring. performing an extraction; After the first hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a first mixed extract; After the first hot water extraction, the remaining solids were filtered with filter paper, and the remaining solids were mixed with water so that the mixing ratio of the solids and water was 0.1 g/ml, and the second hot water extraction was performed at a temperature of 120 ° C for 12 hours while stirring. doing; After secondary hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a secondary mixed extract; After the second hot water extraction, filter with filter paper, and mix the remaining solids with water again so that the mixing ratio of solids and water is 0.1 g/ml, and [BMIm][TFSI](1-Butyl-3-methylimidazolium After adding 1-2 g of trifluoromethylsulfonyl)imide), performing a third hot water extraction at a temperature of 100 ° C. for 8 hours while stirring; After tertiary hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a tertiary mixed extract; and mixing the first mixed extract, the second mixed extract and the tertiary mixed extract.

The Hsagi flower extract, peony flower extract, and evening primrose extract are preferably prepared by a multi-step hot water extraction process, and the active ingredients of each flower can be extracted without damage.

Next, the method for preparing the cosmetic composition for improving atopy according to the present invention includes preparing daffodil oil and lavender oil. The step of preparing daffodil oil and lavender oil may include preparing daffodil powder and lavender powder by pulverizing daffodil and lavender; preparing an extract by mixing a mixture of daffodil powder and lavender powder in a weight ratio of 1:1 with hexane and stirring at a temperature of 33-37 ° C. for 2-4 days; And filtering the extract, concentrating under reduced pressure, centrifuging to remove foreign substances, and then distilling under reduced pressure.

Next, the method for preparing the cosmetic composition for improving atopy according to the present invention includes preparing a device-derived exosome. The step of preparing the device-derived exosome may include washing the device and pre-treating the device at a temperature of 28-32° C. and a pressure of 290-310 MPa for 1-5 minutes; Preparing a device juice by extracting the pretreated device; Obtaining a supernatant containing exosomes by centrifuging the juice from the cow; preparing a lyophilisate by freeze-drying the supernatant containing the exosomes; forming an aqueous two-phase system using polyethylene glycol/dextran in the lyophilisate; and obtaining a lower layer in which exosomes are concentrated in the aqueous two-phase system.

Next, the method for producing a cosmetic composition for improving atopy according to the present invention is Grammostula spatulata mechanotoxin 4 (GsMTx4) peptide 1-5 parts by weight, fresh grass leaf extract prepared above, Hsari 1-5 parts by weight of tree leaf extract and honeysuckle leaf extract, 1-5 parts by weight of clover flower extract, peony flower extract and evening primrose extract prepared above, 0.3-3 parts by weight of exosomes derived from the above-prepared device, niacin 0.5-4 parts by weight of amide, 0.3-3 parts by weight of arginine, 0.3-3 parts by weight of adenosine, 0.3-3 parts by weight of diethylaminohydroxybenzoylhexylbenzoate, 0.3-3 parts by weight of triethylhexanoin, 0.05 parts by weight of stearyl alcohol -0.2 parts by weight, isoquercitrin 0.05-0.2 parts by weight, hyaluronic acid 0.5-4 parts by weight, sodium hyaluronate 0.05-0.2 parts by weight, limonene 0.3-0.7 parts by weight, linalool 0.3-3 parts by weight and preparing a first mixed composition by mixing 47-51 parts by weight of broccoli water and stirring at a temperature of 68-72° C. for 28-32 minutes.

In the above step, the first mixed composition is prepared by mixing the GsMTx4 peptide and the fresh grass leaf extract, etc., and stirring at a relatively high temperature.

The first mixed composition preferably contains 0.3-3 parts by weight of rice bran fermented extract, more preferably 0.5-1.5 parts by weight.

Preparing rice bran powder by washing the fermented rice bran extract with boiled water and pulverizing the rice bran; Adding Saccharomyces cerevisiae as a yeast to a mixture of rice bran powder and water, followed by fermentation at a temperature of 33-37 ° C. for 22-26 hours; Preparing an extract by mixing the fermented mixture and butylene glycol and stirring at a temperature of 28-32 ° C. for 6-8 days; and lyophilizing the extract after filtering and concentrating under reduced pressure.

Preferably, the first mixed composition includes 0.3-3 parts by weight of Jindubal extract, 0.3-3 parts by weight of rhubarb extract, 0.3-3 parts by weight of agar extract, 0.3-3 parts by weight of auditory extract, and 0.3-3 parts by weight of fusiformis extract. And, it is more preferable to include 0.5-1.5 parts by weight of Jindubal extract, 0.5-1.5 parts by weight of rhubarb extract, 0.5-1.5 parts by weight of agar extract, 0.5-1.5 parts by weight of auditory extract, and 0.5-1.5 parts by weight of fusiformis extract.

The step of preparing the Jindubal extract, rhubarb extract, agar-agar extract, auditory extract and fusiformis extract by washing Jindubal, rhubarb, agar-agar, and cutting into appropriate sizes; Raw material powder was obtained by mixing and pulverizing washed jindubal, rhubarb, agar-agar, sage and fusiformis in a weight ratio of 1: 1: 1: 1: 1, and 8-12 parts by weight of the raw material powder and 88-92 parts by weight of water Heating the mixed mixture to a temperature of 92-95° C., cooling the evaporating vapor, and mixing the distillate with the mixture again to circulate for 90-150 minutes; obtaining a primary extract by heating the cycled mixture to a temperature of 98-100° C. and cooling the evaporating vapor for 150-210 minutes; Obtaining a solid content of the mixture, mixing it with ethanol, and then extracting at a temperature of 48-52 ° C. for 2-6 hours to obtain a secondary extract; and concentrating the first extract and the second extract under reduced pressure, followed by lyophilization and pulverization.

Next, the method for producing the cosmetic composition for improving atopy according to the present invention includes 0.5-4 parts by weight of daffodil oil and lavender oil prepared above, 3-7 parts by weight of squalane, 2-6 parts by weight of butylene glycol, propylene 0.05-0.2 parts by weight of glycol, 0.05-0.2 parts by weight of polyglyceryl-6 stearate, and 18-22 parts by weight of broccoli water were mixed and stirred at a temperature of 38-42 ° C. for 58-62 minutes to obtain a second mixed composition It includes the step of manufacturing.

In the above step, ingredients such as sea daffodil oil, lavender oil, and squalane are mixed, but stirred at a relatively low temperature to prepare a second mixed composition.

Next, the manufacturing method of the cosmetic composition for improving atopy according to the present invention includes mixing the first mixed composition and the second mixed composition, and stirring at a temperature of 48-52 ° C. for 18-22 minutes.

Finally, a whitening cosmetic composition is prepared by mixing and stirring the first mixed composition and the second mixed composition.

Hereinafter, the present invention will be described in more detail by the following examples.

However, the following examples are merely illustrative of the content of the present invention, but the scope of the invention is not limited by the examples and experimental examples.

<Preparation Example 1> Preparation of fresh herb leaf extract, clover clover leaf extract and honeysuckle leaf extract

Fresh herb leaves, clover clover leaves and honeysuckle leaves were washed, freeze-dried, and pulverized to prepare fresh herb leaf powder, clover clover leaf powder, and honeysuckle leaf powder. Prepared fresh herb leaf powder, clover leaf powder and honeysuckle leaf powder were mixed in a weight ratio of 1: 1: 1, and a mixed solvent in which ethanol and hexane were mixed in a volume ratio of 1: 1 and a weight ratio of 1: 9 , and extracted for 24 hours at a temperature of 30 ° C. to prepare an extract. The extract was filtered with a filter paper, and the filtered filtrate was concentrated under reduced pressure and lyophilized at -50° C. to prepare an extract of cinnamon plant leaf, clover clover leaf extract, and honeysuckle leaf extract.

<Preparation Example 2> Preparation of clover flower extract, peony flower extract and evening primrose extract

After washing clover flower, peony flower, and evening primrose, freeze-drying, and pulverizing to prepare clover flower powder, peony flower powder, and evening primrose powder, the prepared clover flower powder, peony flower powder, and evening primrose powder were prepared 1:1: 20 g of the mixed powder mixed at a weight ratio of 1 was mixed with 200 ml of water, and primary hot water extraction was performed at a temperature of 80° C. for 8 hours while stirring. After the first hot water extraction, the mixture was filtered with filter paper, and the filtrate was freeze-dried to prepare a first mixed extract.

After the first hot water extraction, the remaining solids were filtered with filter paper, and the remaining solids were mixed with water so that the mixing ratio of the solids and water was 0.1 g/ml, and the second hot water extraction was performed at a temperature of 120 ° C for 12 hours while stirring. did After the second hot water extraction, the mixture was filtered with filter paper, and the filtrate was freeze-dried to prepare a second mixed extract.

After the second hot water extraction, filter with filter paper, and mix the remaining solids with water again so that the mixing ratio of solids and water is 0.1 g/ml, and [BMIm][TFSI](1-Butyl-3-methylimidazolium After adding 1.5 g of trifluoromethylsulfonyl)imide), tertiary hot water extraction was performed at a temperature of 100° C. for 8 hours while stirring. After the tertiary hot water extraction, the mixture was filtered with a filter paper, and the filtrate was freeze-dried to prepare a tertiary mixed extract.

The first mixed extract, the second mixed extract, and the tertiary mixed extract were mixed to prepare a clover flower extract, a peony flower extract, and an evening primrose extract.

<Preparation Example 3> Preparation of daffodil oil and lavender oil

Sea daffodil and lavender were pulverized to prepare daffodil powder and lavender powder. A mixed powder was prepared by mixing the daffodil powder and lavender powder in a weight ratio of 1:1. An extract was prepared by mixing 10 g of the mixed powder and 90 g of hexane and stirring at a temperature of 35° C. for 3 days. After filtering and concentrating under reduced pressure, the process of centrifuging to remove foreign substances was repeated. By repeating the last vacuum distillation, residual solvent, hexane, was removed to less than 0.1 ppm, and through this, daffodil oil and lavender oil were prepared.

<Preparation Example 4> Preparation of element-derived exosomes

After cleaning the device, it was placed in an ultra-high pressure machine and pretreated at a temperature of 30° C. and a pressure of 300 MPa for 3 minutes. The pretreated element was juiced using a juicer to prepare a element juice. After centrifuging the soybean juice at 10,000xg for 10 minutes, a supernatant was obtained. The supernatant was freeze-dried at -50 °C for 8 hours to prepare a lyophilisate. An aqueous two-phase system was formed using an aqueous solution containing 3.3% by weight of polyethylene glycol having a weight average molecular weight of 10,000 to 35,000 and 1.7% by weight of dextran having a weight average molecular weight of 400,000 to 500,000 to the lyophilisate. A lower layer in which exosomes were concentrated in the aqueous two-phase system was obtained to prepare device-derived exosomes.

<Preparation Example 5> Preparation of fermented rice bran extract

Rice bran was washed three times with boiled water and ground to prepare rice bran powder. A mixture was prepared by mixing 10 g of rice bran powder and 300 g of water, and after adding 0.3 g of Saccharomyces cerevisiae as a yeast to the mixture, the mixture was fermented at 35° C. for 24 hours. An extract was prepared by mixing 300 g of butylene glycol with the fermented mixture and stirring at a temperature of 30° C. for 7 days. The extract was filtered and concentrated under reduced pressure, and then freeze-dried to prepare a fermented rice bran extract.

<Preparation Example 6> Preparation of Jindubal extract, rhubarb extract, agar-agar extract, auditory extract and fusiformis extract

Prepare by washing Jindubal, Rhubarb, Agar-agar, Agar and Hijiki by cutting them into appropriate sizes, and mixing and grinding the washed Jindubal, Rhubarb, Agar-agar, Agaric and Hijiki at a weight ratio of 1:1:1:1:1 to prepare a first mixture. A second mixture is prepared by mixing 10 parts by weight of the first mixture and 90 parts by weight of water, the second mixture is heated to a temperature of 95 ° C, and the distillate obtained by cooling the evaporating vapor is mixed with the mixture again Circulated for 120 minutes. The second mixture after completion of the circulation was heated to a temperature of 98-100° C., and the evaporating vapor was cooled for 180 minutes to obtain a first extract.

The solid content of the second mixture was obtained and mixed with ethanol, followed by extraction at a temperature of 50° C. for 4 hours to obtain a secondary extract.

After concentrating the first extract and the second extract under reduced pressure, lyophilization and pulverization were performed to prepare Jindubal extract, rhubarb extract, agar-agar extract, auditory extract and fusiformis extract.

<Example 1-3 and Comparative Example 1> Preparation of cosmetic composition for improving atopy

Grammostula spatulata mechanotoxin 4 (GsMTx4) peptide, fresh grass leaf extract prepared in Preparation Example 1, clover clover leaf extract and honeysuckle leaf extract, clover clover prepared in Preparation Example 2 Flower extract, Peony flower extract and Evening primrose extract, Soja-derived exosomes prepared in Preparation Example 4, Rice bran fermented extract prepared in Preparation Example 5, Jindubal extract prepared in Preparation Example 6, Rhubarb extract, Agar-agar extract, Auditory Extract and Hijiki Extract, Niacinamide, Arginine, Adenosine, Diethylaminohydroxybenzoylhexylbenzoate, Triethylhexanoin, Stearyl Alcohol, Isoquercitrin, Hyaluronic Acid, Sodium Hyaluronate, Limonene, A first mixed composition was prepared by mixing linalool and broccoli water and stirring at a temperature of 70° C. for 30 minutes.

Daffodil oil and lavender oil, squalane, butylene glycol, propylene glycol, polyglyceryl-6 stearate, and broccoli water prepared in Preparation Example 3 were mixed and stirred at a temperature of 40 ° C. for 60 minutes To prepare a second mixed composition.

The first mixed composition and the second mixed composition were mixed and stirred at a temperature of 50 ° C. for 20 minutes to prepare a cosmetic composition for improving atopy.

The composition of the cosmetic composition for improving atopy is shown in Table 1 below.

Example 1 Example 2 Example 3 Comparative Example 1 Preparation Example 1 3 3 3 - Preparation Example 2 3 3 3 - Preparation Example 3 2 2 2 - Production Example 4 One One One - Preparation Example 5 - One One - Preparation Example 6 - - 5 - GsMTx4 peptide 3 3 3 3 Niacinamide 2 2 2 2 arginine One One One One adenosine One One One One Diethylaminohydroxybenzoylhexylbenzoate One One One One triethylhexanoin One One One One squalane 5 5 5 5 Butylene Glycol 4 4 4 4 propylene glycol 0.1 0.1 0.1 0.1 Polyglyceryl-6 stearate 0.1 0.1 0.1 0.1 Stearyl Alcohol 0.1 0.1 0.1 0.1 isoquercitrin 0.1 0.1 0.1 0.1 Hyaluronic Acid 2 2 2 2 sodium hyaluronate 0.1 0.1 0.1 0.1 limonene 0.5 0.5 0.5 0.5 linalool One One One One broccoli water 69 69 69 69

<Experimental Example 1> Skin irritation test

A skin test was conducted to confirm the degree of skin irritation of the cosmetic compositions prepared in Examples 1-3 and Comparative Example 1. For the skin test, a total of 20 men and women, 5 people from each age group from 10 to 40 years of age, applied the cosmetic composition to the upper arm area, and 1 hour later, the skin reaction was first visually inspected, and 24 hours later, a second visual inspection was performed. The results are shown in Table 2 below.

(○: No skin change, △: Slight erythema appeared, ×: Clear erythema appeared)

degree of skin irritation Example 1 1st:○ , 2nd:○ Example 2 1st:○ , 2nd:○ Example 3 1st:○ , 2nd:○ Comparative Example 1 1st:○ , 2nd:○

As shown in Table 2, it was confirmed that there was no abnormality as a result of the skin irritation test.

<Experimental Example 2> Atopy improvement experiment

Experiments were conducted to confirm the effect of alleviating the symptoms of atopic dermatitis of the cosmetic compositions prepared in Examples 1-3 and Comparative Example 1. To this end, 80 atopic skin disease patients were divided into 4 groups of 20 each, 1 group received the cosmetic composition of Example 1, 2 groups received the cosmetic composition of Example 2, 3 groups received the cosmetic composition of Example 3, and 4 groups received the cosmetic composition of Example 2. In, the cosmetic composition of Example 4 was used for 30 days to analyze the itching relief and atopy improvement effects, and the results are shown in Table 3 below. Itching relief was evaluated on a 5-point scale, and the atopy improvement effect was evaluated on a 0-100% scale.

For itching relief, 1 point is very bad, 2 points are bad, 3 points are average, 4 points are good, 5 points are very good, and 0% is no healing effect and 25% is slightly improved for the atopy improvement effect. , 50% is moderate improvement, 75% is significant improvement, and 100% is complete healing.

5 days 10 days 15th 30 days Example 1 itching relief 4.0 4.2 4.3 4.5 Atopic improvement (%) 46.3 58.9 73.2 87.4 Example 2 itching relief 4.2 4.3 4.5 4.6 Atopic improvement (%) 48.1 62.3 76.0 90.2 Example 3 itching relief 4.3 4.4 4.6 4.8 Atopic improvement (%) 50.3 68.2 83.6 94.6 Comparative Example 1 itching relief 3.8 3.8 3.7 3.8 Atopic improvement (%) 8.5 10.2 14.6 18.6

As shown in Table 3, it was confirmed that the cosmetic composition for improving atopy according to the present invention has an effect of alleviating and improving itching for atopic dermatitis.

Claims (5)

Grammostula spatulata mechanotoxin 4 (GsMTx4) peptide 1-5 parts by weight, fresh grass leaf extract 0.3-3 parts by weight, clover clover leaf extract 0.3-3 parts by weight, honeysuckle leaf extract 0.3-3 parts by weight 3 parts by weight, clover flower extract 0.3-3 parts by weight, peony flower extract 0.3-3 parts by weight, evening primrose extract 0.3-3 parts by weight, sea daffodil oil 0.3-3 parts by weight, lavender oil 0.3-3 parts by weight, derived from Soja Exosome 0.3-3 parts by weight, niacinamide 0.5-4 parts by weight, arginine 0.3-3 parts by weight, adenosine 0.3-3 parts by weight, diethylaminohydroxybenzoylhexylbenzoate 0.3-3 parts by weight, triethylhexanoin 0.3 -3 parts by weight, squalane 3-7 parts by weight, butylene glycol 2-6 parts by weight, propylene glycol 0.05-0.2 parts by weight, polyglyceryl-6 stearate 0.05-0.2 parts by weight, stearyl alcohol 0.05- 0.2 parts by weight, isoquercitrin 0.05-0.2 parts by weight, hyaluronic acid 0.5-4 parts by weight, sodium hyaluronate 0.05-0.2 parts by weight, limonene 0.3-0.7 parts by weight, linalool 0.3-3 parts by weight, and A cosmetic composition for improving atopy, comprising 67-71 parts by weight of broccoli water. delete According to claim 1,
The cosmetic composition for improving atopy,
2-4 parts by weight of Grammostula spatulata mechanotoxin 4 (GsMTx4) peptide, 0.5-1.5 parts by weight of fresh grass leaf extract, 0.5-1.5 parts by weight of clover leaf extract, 0.5-1.5 parts by weight of honeysuckle leaf extract 1.5 parts by weight, clover flower extract 0.5-1.5 parts by weight, peony flower extract 0.5-1.5 parts by weight, evening primrose extract 0.5-1.5 parts by weight, sea daffodil oil 0.5-1.5 parts by weight, lavender oil 0.5-1.5 parts by weight, Soja Exosome 0.5-1.5 parts by weight, niacinamide 1-3 parts by weight, arginine 0.5-1.5 parts by weight, adenosine 0.5-1.5 parts by weight, diethylaminohydroxybenzoylhexylbenzoate 0.5-1.5 parts by weight, triethylhexanoin 0.5 parts by weight -1.5 parts by weight, squalane 4-6 parts by weight, butylene glycol 3-5 parts by weight, propylene glycol 0.08-0.12 parts by weight, polyglyceryl-6 stearate 0.08-0.12 parts by weight, stearyl alcohol 0.08- 0.12 parts by weight, isoquercitrin 0.08-0.12 parts by weight, hyaluronic acid 1-3 parts by weight, sodium hyaluronate 0.08-0.12 parts by weight, limonene 0.4-0.6 parts by weight, linalool 0.5-1.5 parts by weight and A cosmetic composition for improving atopy, comprising 68-70 parts by weight of broccoli water. Preparing fresh herb leaf powder, clover clover leaf powder, and honeysuckle leaf powder by washing, freeze-drying, and pulverizing fresh herb leaves, clover clover leaf and honeysuckle leaf; Prepared fresh herb leaf powder, clover leaf powder and honeysuckle leaf powder were mixed in a weight ratio of 1: 1: 1, and a mixed solvent in which ethanol and hexane were mixed in a volume ratio of 1: 1 and a weight ratio of 1: 9 preparing an extract by mixing and extracting at a temperature of 28-32 ° C for 22-26 hours; and filtering the extract with a filter paper, concentrating the filtered filtrate under reduced pressure and freeze-drying the filtrate at minus 50 ° C.
Preparing clover flower powder, peony flower powder, and evening primrose powder by washing, freeze-drying, and pulverizing clover flower, peony flower, and evening primrose; 20 g of the prepared mixed powder of clover flower powder, peony flower powder, and evening primrose powder mixed in a weight ratio of 1:1:1 was mixed with 200 ml of water, followed by primary hot water at 80°C for 8 hours while stirring. performing an extraction; After the first hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a first mixed extract; After the first hot water extraction, the remaining solids were filtered with filter paper, and the remaining solids were mixed with water so that the mixing ratio of the solids and water was 0.1 g/ml, and the second hot water extraction was performed at a temperature of 120 ° C for 12 hours while stirring. doing; After secondary hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a secondary mixed extract; After the second hot water extraction, filter with filter paper, and mix the remaining solids with water again so that the mixing ratio of solids and water is 0.1 g/ml, and [BMIm][TFSI](1-Butyl-3-methylimidazolium After adding 1-2 g of trifluoromethylsulfonyl)imide), performing a third hot water extraction at a temperature of 100 ° C. for 8 hours while stirring; After tertiary hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a tertiary mixed extract; And mixing the first mixed extract, the second mixed extract and the tertiary mixed extract; preparing a bush clover flower extract, a peony flower extract and an evening primrose extract containing;
Preparing sea daffodil powder and lavender powder by crushing sea daffodil and lavender; preparing an extract by mixing a mixture of daffodil powder and lavender powder in a weight ratio of 1:1 with hexane and stirring at a temperature of 33-37 ° C. for 2-4 days; Preparing sea daffodil oil and lavender oil comprising; filtering and concentrating the extract under reduced pressure, centrifuging to remove foreign substances, and distilling under reduced pressure;
washing the device and pre-treating it at a temperature of 28-32° C. and a pressure of 290-310 MPa for 1-5 minutes; Preparing a device juice by extracting the pretreated device; Obtaining a supernatant containing exosomes by centrifuging the juice from the cow; preparing a lyophilisate by freeze-drying the supernatant containing the exosomes; forming an aqueous two-phase system using polyethylene glycol/dextran in the lyophilisate; and obtaining an exosome-enriched lower layer in the aqueous two-phase system; preparing a device-derived exosome comprising;
1-5 parts by weight of Grammostula spatulata mechanotoxin 4 (GsMTx4) peptide, 1-5 parts by weight of the above-prepared cinnamon leaf extract, clover clover leaf extract and honeysuckle leaf extract, 1-5 parts by weight of Hsagi flower extract, peony flower extract and evening primrose extract prepared above, 0.3-3 parts by weight of the device-derived exosome prepared above, 0.5-4 parts by weight of niacinamide, 0.3-3 parts by weight of arginine, 0.3 parts by weight of adenosine -3 parts by weight, diethylaminohydroxybenzoylhexylbenzoate 0.3-3 parts by weight, triethylhexanoin 0.3-3 parts by weight, stearyl alcohol 0.05-0.2 parts by weight, isoquercitrin 0.05-0.2 parts by weight, hyaluronic acid 0.5-4 parts by weight of rhonic acid, 0.05-0.2 parts by weight of sodium hyaluronate, 0.3-0.7 parts by weight of limonene, 0.3-3 parts by weight of linalool and 47-51 parts by weight of broccoli water were mixed, and Stirring for 28-32 minutes at a temperature to prepare a first mixed composition;
0.5-4 parts by weight of daffodil oil and lavender oil prepared above, 3-7 parts by weight of squalane, 2-6 parts by weight of butylene glycol, 0.05-0.2 parts by weight of propylene glycol, polyglyceryl-6 stearate Preparing a second mixed composition by mixing 0.05-0.2 parts by weight and 18-22 parts by weight of broccoli water and stirring at a temperature of 38-42 ° C. for 58-62 minutes; and
Mixing the first mixed composition and the second mixed composition, and stirring at a temperature of 48-52 ° C. for 18-22 minutes; Method for producing a cosmetic composition for improving atopy comprising the. According to claim 4,
The first mixed composition is a method for producing a cosmetic composition for improving atopy, characterized in that it comprises 0.3-3 parts by weight of rice bran fermentation extract.

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