KR102513350B1 - Centrifugal dehydrator - Google Patents

Abstract

The centrifugal separation device of the present invention is a centrifugal separation device for centrifuging a biological sample by rotating a syringe composed of a syringe containing an acquired biological sample, a first holder and a second holder sealed at the tip and end of the syringe, respectively, A rotating body, a bracket that interlocks with the rotating body and accommodates a portion of the tip in a state in which the syringe is installed vertically, and rotates the syringe around its long axis, and a state in which the syringe rotates and the inside of the syringe is sealed and a sample processing unit for supplying fluid to the inside, washing unnecessary samples among biological samples in a syringe after centrifugation, and carrying them out.

Description

Centrifugal separator {CENTRIFUGAL DEHYDRATOR}

The present invention relates to a centrifugal separator, and more particularly, by rotating a syringe in which a biological sample is obtained based on its long axis, a necessary sample is located on the inner wall of a syringe, and unnecessary samples are separated so that they are gathered in the center of the syringe so that unnecessary samples are collected through a sample processing unit. It relates to a centrifugal separation device that facilitates washing and taking out samples.

Fat grafting is not just using fat collected through liposuction as in the past, but is a process of separating only living fat cells through centrifugation and washing.

The key is to drastically improve the survival rate by introducing a technology that separates only living fat cells, unlike oil from destroyed fat cells and blood generated during inhalation that causes necrosis of the surrounding transplanted fat tissue.

In the process of fat transplantation, a small incision is made on the body part, a tube with a hole at the end (liposuction tube) is inserted, and fat is sucked out using negative pressure from a syringe.

Thereafter, liquid components such as adipose stem cells, red blood cells, blood, and moisture are separated into layers from the fat collected in the syringe through a centrifugal separator, and free oil separated from the red blood cells and broken fat cells separated from the layers in the syringe. (free oil) is removed, and only the adipose stem cells to be transplanted are acquired and injected into the human body.

To achieve this, the syringe from which the fat was extracted is transferred to a test tube for a centrifugal separator under positive pressure, and then the tube is installed in a centrifuge and rotated at high speed to separate the fat substances according to the size and density of the particles. (erythrocytes, free oil) are separated.

Here, centrifugation uses the difference in density between a solid and a liquid surrounding the solid. By placing a mixture in a test tube and rotating it rapidly, artificially giving it a strong acceleration, the heavier material sinks at the bottom of the tube and the lighter material floats on top.

That is, as the aspirated fat is subjected to centrifugation, as shown in FIG. 1, in the test tube, red blood cells E are located at the lowest layer, and free oil F is located at the top layer, and in the middle between the red blood cells E and free oil F Adipose stem cells S are divided into layers.

Then, only the adipose stem cell S is extracted in the test tube through a syringe equipped with a needle.

However, the prior art has a problem in that secondary infection due to the risk of microbial contamination may occur as the extracted fat is exposed to external air in the process of transferring the initially extracted fat to a test tube through a syringe.

In addition, due to the disadvantage of using expensive equipment such as a clean bench or a clean room to prevent such a risk of contamination, the device and method for obtaining adipose stem cells S are complex and difficult.

In addition, when extracting only the adipose stem cell S in the test tube, there is a possibility of inflow of foreign substances and exposure to the outside air, which can cause secondary infection by air or foreign substances, making it difficult to perform an aseptic extraction process. .

This requires time and labor in the extraction method of adipose stem cell S, and there is a risk of infection and loss of pure fat due to air contact.

Specifically, among several medical procedures starting in the 1990s, such as fat transplantation and PRP enriched transplantation, a medical syringe was used as a centrifugal separation vessel to eliminate the inconvenience of transferring to a new centrifugal separation vessel and to minimize contamination of airborne bacteria. system is being used.

As a tool other than the necessary syringe for this purpose, a sterilizable and detachable syringe bucket is essential.

Since it is very inconvenient for medical personnel wearing sterile gloves to change gloves for centrifugation and increases the possibility of contamination, all parts of the equipment that comes into contact with medical personnel are sterilized in advance and centrifuged by an aseptic protocol before use. because it has to be installed.

At this time, the centrifugal separation method used is a method in which the long axis of the vessel is placed on the rotating surface or at a certain angle, so it is not easy to achieve a continuous flow of fluid during rotation and the inside of the rotating vessel cannot be observed. The problem is that it is difficult to predict.

As shown in FIG. 2, when platelets, leukocytes, stem cells, etc. are to be separated from the blood, the process of reducing red blood cells cannot be observed while the hemolysis buffer that is 500 times more than the amount of blood is continuously passed through. It was difficult to set the separation time, and there was no case where this method was actually applied.

[Patent 0001] Republic of Korea Patent Publication No. 2013-0062689, (2013.06.13) [Patent 0002] Republic of Korea Patent Publication No. 2015-0051026, (2015.05.11)

Accordingly, an object of the present invention is to enable centrifugal separation by attaching the syringe as it is to a centrifuge without transferring a sample extracted from a human body through a syringe to a test tube.

In addition, an object of the present invention is to rotate a syringe in which a biological sample is obtained based on a long axis so that a necessary sample is located on the inner wall of the syringe and unnecessary samples are gathered in the center of the syringe so that unnecessary samples can be washed and taken out through the sample processing unit. is to make it easy.

In order to achieve the above object, the centrifugal separator of the present invention rotates a syringe composed of a syringe containing an acquired biological sample, and a first holder and a second holder that seal the tip and end of the syringe, respectively, to centrifuge the biological sample. A centrifugal separator for separating a rotating body, a bracket that interlocks with the rotating body and accommodates a portion of the tip of the syringe in a state in which the syringe is installed vertically, and rotates the syringe about a long axis, and supports the rotation of the syringe. and a sample processing unit that supplies fluid to the inside of the syringe in a state in which the inside of the syringe is sealed, cleans unnecessary samples among the biological samples after centrifugation, and carries them out of the syringe.

According to the present invention, the upper portion of the bracket is opened to form a multi-stage groove that partially accommodates the tip of the syringe, and the multi-stage groove is concentrically formed with first grooves and second grooves having different diameters. 1 holder is accommodated, and a part of the syringe is accommodated in the second groove.

According to the present invention, the rotating body and the bracket can be separated or combined.

According to the present invention, the sample processing unit includes a cleaning means for supplying a washing liquid through an inlet pipe coupled to the syringe, and a removal means for sucking the washing liquid and unnecessary samples through an outlet pipe coupled to the syringe.

According to the present invention, an idle rotation means is further included in the center of the second holder to prevent twisting of the syringe and the sample processing unit.

According to the present invention, a power unit for transmitting rotational force to the rotating body is further included.

According to the present invention, the connecting means is coupled vertically to the power unit, and the upper portion of the connecting means grips the sample processing unit.

According to the present invention, the second groove further includes a pressing member made of an elastic material on the inner periphery so that the syringe is elastically fitted into the second groove.

According to the present invention, the washing means further includes a washing liquid supply means for pressurizing the washing water, and the first conduit connected to the washing liquid supply means is connected to the inlet pipe.

According to the present invention, the removal means further includes a waste means for generating vacuum pressure to take the unnecessary sample and washing water inside the syringe to the outside, and the second conduit connected to the waste means and the outlet pipe are connected. .

According to the present invention, the inlet pipe and the outlet pipe further include a filter.

According to the present invention, the filter is detachable from the outlet pipe and the inlet pipe.

According to the present invention, the filter is formed with a hole in the unit of 30 ~ 500um.

As described above, the centrifugal separator for a syringe of the present invention centrifugally separates a biological sample by rotating a syringe composed of a syringe containing an acquired biological sample, a first holder and a second holder that seal the tip and tip of the syringe, respectively. A centrifugal separation device comprising a rotating body, a bracket that interlocks with the rotating body and accommodates a portion of the tip in a state in which the syringe is installed vertically to rotate the syringe around a long axis, and supports rotation of the syringe, and a sample processing unit which supplies fluid to the inside of the syringe in a state in which the inside of the syringe is hermetically sealed and, after centrifugation, cleans unnecessary samples among the biological samples from the syringe and carries them out.

Through this, there is an effect that the sample extracted from the human body through the syringe can be centrifuged by attaching the syringe to the centrifuge without transferring it to the test tube.

In addition, the syringe obtained with the biological sample is rotated on the long axis, so that the necessary sample is located on the inner wall of the syringe and unnecessary samples are gathered in the center of the syringe, so that the unnecessary sample can be easily washed and taken out through the sample processing unit. there is.

1 is a perspective view showing a syringe for a sample applied to centrifugation.
Figure 2 is a perspective view showing a centrifugal separator according to the prior art.
3 is a perspective view showing a state in which a syringe is mounted on a centrifugal separator according to the prior art;
4 is a front sectional view showing the centrifugal separator of the present invention;
5 is a front sectional view showing a centrifugal separation step using the centrifugal separator of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

First of all, it should be noted that among the drawings, the same components or parts are indicated by the same reference numerals as much as possible. In describing the present invention, detailed descriptions of related known functions or configurations are omitted in order not to obscure the gist of the present invention.

4 is a front cross-sectional view showing the centrifugal separator of the present invention, and FIG. 5 is a front cross-sectional view showing a centrifugal separation step using the centrifugal separator of the present invention.

As shown in FIGS. 4 and 5, the centrifugal separator 10 of the present invention vertically places the injector 100 containing the biological sample in the centrifugal separator 10 and rotates the injector 100 about its long axis to centrifugally separate the biological sample and unnecessary Take the sample outside and clean the inside of the syringe 100.

Here, the syringe 100 is coupled with the syringe 110 containing the obtained biological sample, and the first holder 120 and the second holder 130 that seal the tip and end of the syringe 110, respectively.

The space of the syringe 110 is sealed through the first holder 120 and the second holder 130 .

The configuration of the device for centrifuging the injector 100 is as follows.

The centrifugal separator 10 includes a power unit 500, a rotating body 200, a bracket 300, and a sample processing unit 400.

The power unit 500 is applied with a motor that generates a rotational force, and the rotational axis of the motor is vertically positioned at the top.

In addition, the rotating body 200 is connected to the rotating shaft so that the rotating body 200 rotates through the rotational force of the power unit 500.

Also, a bracket 300 is further coupled to the top of the rotating body 200.

The bracket 300 interlocks with the rotating body 200 and rotates the syringe 110 around its long axis by accommodating a portion of the tip in a state where the syringe 110 is installed vertically.

The upper part of the bracket 300 is open to form a multi-stage groove 310 that partially accommodates the tip of the syringe 110. The first holder 120 is accommodated in the holder, and a part of the syringe 110 is accommodated in the second groove 312.

That is, the first groove 311 and the second groove 312 are continuously formed in multiple stages so that the outer circumference of the first holder 120 and the syringe 110 can accommodate the external shape of the syringe 100 of the bracket 300 without interference. To be coupled to the groove 312, respectively.

Here, the rotating body 200 and the bracket 300 can be separated or combined. This applies when performing regular disinfection of the bracket 300.

At this time, in order to prevent arbitrary separation of the syringe 100 coupled to the bracket 300, a pressing member 313 made of an elastic material is installed on the inner periphery of the second groove 312.

Elastic rubber may be applied to the pressing member 313 so that the syringe 110 is elastically fitted into the second groove 312 through the pressing member 313 .

In addition, the sample processing unit 400 supports the rotation of the syringe 110 and supplies fluid to the inside of the syringe 110 in a state where the inside of the syringe 110 is sealed, and after centrifugation, unnecessary samples among the biological samples are washed in the syringe 110 and taken out.

The sample processing unit 400 is composed of a washing means 410 for supplying washing liquid through an inlet pipe 413 coupled to the inside of the syringe 110, and a removal means 420 for sucking in washing liquid and unnecessary samples through an outlet pipe 423 coupled to the inside of the syringe 110. do.

Here, the washing means 410 further includes a washing liquid supply means 411 for pressurizing the washing water, and a first conduit 412 connected to the washing liquid supply means 411 and an inlet pipe 413 are connected.

In addition, the removal means 420 further includes a waste means 421 generating vacuum pressure to take the unwanted sample and washing water inside the syringe 110 to the outside, and a second conduit 422 and an outlet pipe 423 connected to the waste means 421. this is connected

After centrifugation, unnecessary samples are filled inside the syringe 110, except for the necessary samples attached to the inner wall of the syringe 110, and washing liquid is supplied into the syringe 110 through the inlet pipe 413 to lower the viscosity of unnecessary samples. Then, through the outlet pipe 423, the unnecessary sample diluted in the washing liquid is sucked in with vacuum pressure and taken out.

The filter 440 is coupled to the outlet pipe 423 and the inlet pipe 413 to be detachable.

Here, the longest length of the outflow pipe 423 and the inlet pipe 413 is 50 cm or less, considering general sizes of medical high-pressure steam sterilizers, EO gas sterilizers, plasma sterilizers, and boiling sterilizers.

In particular, the outflow pipe 423 and the inlet pipe 413 are polymers (PE, PP, PC, Teflon, silicone, ABS, MA, acetal, acrylic, polystyrene, etc.) that are stable to heat of 80 degrees or more and do not react to disinfection gas, plasma, or the like. made only of metal

Here, the filter 440 is formed with holes of 30 to 500 μm.

In addition, an idler 430 is further included in the center of the second holder 130 to prevent twisting of the syringe 110 and the sample processing unit 400.

Here, the idling means 430 can be applied as a bearing, so that the outflow pipe 423 and the inlet pipe 413 are prevented from twisting by idling in the second holder 130 interlocking with the syringe 110.

The centrifugal separator 10 configured as described above rotates the long axis of the transparent cylindrical syringe 100 as a rotation axis through the centrifugal separation step, so that unnecessary samples are collected on the inner wall of the syringe 110 and the thickness of the layer can be observed with the naked eye, so that the centrifugation time It is advantageous in setting and can easily form a fluid flow path in the central part, so that processes such as continuous washing can be performed.

A method of centrifuging using the centrifugal separator 10 configured as described above is as follows.

A typical target procedure for centrifugation is stromal vascular fraction (SVF) or platelet-rich plasma (PRP/Platelet-Rich Plasma).

First, when the stromal stem cell fractionation procedure is performed using the centrifugal separator 10 of the present invention, the sample syringe 100 is used for collection in the process of dissolving human tissue in proteolytic enzyme and then separating only the cells, and then the syringe 100 is not replaced. The probability of contamination is reduced by using the method of using the enzyme reaction vessel as it is.

However, it is still a problem that the process of final washing of the enzyme is complicated and takes a long time to wash, and during the washing time, the cells already separated attach to the undissolved tissue and are lost together in the process of removing the undissolved tissue. It becomes.

Therefore, there is an effect of simplifying the washing process and minimizing the washing time while easily reprocessing the undigested tissue.

In addition, when the platelet-rich plasma procedure is performed using the centrifuge 10 of the present invention, the blood contained in the syringe 100 is transferred to a special container and subjected to centrifugation. , stem cells, and platelets exist in between.

The problem with this method is that during centrifugation, it is difficult to remove only the platelet layer formed of a thin, buffy coat of platelets buried together with red blood cells.

If only serum and red blood cells can be removed from blood, platelets and leukocyte stem cells can be obtained together. To test a small amount of sample, red blood cells are removed using a low osmotic buffer for hemolysis of red blood cells.

The problem with this red blood cell removal method is that about 500 cc of buffer washing is required for 1 cc of blood, and since more than 20 cc of blood is usually used, 20,000 cc of washing liquid is required, and it takes a lot of time to concentrate other cells from this amount of washing liquid. that it needs

Therefore, if a long-axis rotary centrifuge for washing and collecting a large amount of sludge through the sample processing unit 400 of the present invention is applied, the red blood cell lysis method is effective in collecting other blood cells.

Technical features of the centrifugal separator 10 configured as above are as follows.

First, the transparent medical injector 100 treated aseptically through a centrifugal separator is allowed to rotate in its long axis.

Through this, there is an effect of easily applying the syringe 100, which is a disposable container required in an aseptic process, and separating and combining with the centrifugal separator 10 easily.

Ultimately, the stand-alone medical syringe 100 is used as part of the centrifuge without the need to move the container.

In addition, the outflow pipe 423 and the inlet pipe 413 are prevented from twisting in a state in which rotational friction is reduced through the idler 430 .

In addition, even if the washing liquid is continuously flowed through the inlet pipe 413, the biological sample contained in the syringe 100 is not lost because it is spread and fixed to the inner wall of the syringe 100. Therefore, there is an effect that it is easy and takes less time to wash a large amount of biological samples or dilute impurities.

especially. Since the necessary sample to be collected is attached to the wall of the syringe 100, the unnecessary sample is collected in the center except for the inner wall of the syringe 110, so the supernatant can be removed while looking inside in the centrifugal state, so impurities are easily removed.

The present invention described above is not limited by the foregoing embodiments and the accompanying drawings, and various substitutions, modifications, and changes are possible within a range that does not deviate from the technical spirit of the present invention. It will be clear to those who have knowledge of

10: centrifugal separator 100: syringe
110: syringe 120: first holder
130: second holder 200: rotating body
300: bracket 310: multi-stage home
311: first home 312: second home
313: pressure member 400: sample processing unit
410: washing means 411: washing liquid supply means
412: first pipeline 413: inlet pipe
420: Removal means 421: Disposal means
422: second pipeline 423: outflow pipe
430: idling means 440: filter
500: power unit 510: connection means

Claims (13)

A centrifugal separator for centrifuging a biological sample by rotating a syringe composed of a syringe containing an obtained biological sample, a first holder and a second holder sealing the tip and tip of the syringe, respectively;
A rotating body further comprising a power unit for transmitting rotational force;
The power unit has a connecting means coupled vertically, and a sample processing unit on top of the connecting means;
It consists of a washing means for supplying washing liquid through an inlet pipe coupled to the inside of the syringe, and a removal means for sucking in washing liquid and unnecessary samples through an outflow pipe coupled to the inside of the syringe, wherein the washing means pressurizes the washing water. A washing liquid supply means is further included so that the inlet pipe is connected to the first conduit connected to the washing liquid supply means, and the removal means is a waste disposal means that generates vacuum pressure to take the unnecessary sample and washing water inside the syringe to the outside. A sample processing unit further included and connected to a second conduit connected to the disposal means and an outflow pipe;
The upper part is opened to form a multi-stage groove that partially accommodates the syringe tip so that the syringe can be rotated around a long axis by accommodating a portion of the tip in a state in which the syringe is interlocked with the rotating body and the syringe is installed vertically, the multi-stage groove A first groove and a second groove having different diameters are formed concentrically, the first groove accommodates the first holder, and the second groove further includes a pressure member made of an elastic material on the inner periphery so that the syringe is attached to the second groove. A centrifugal separator, characterized in that it is composed of a bracket configured to be elastically fitted.
delete According to claim 1,
The centrifugal separator, characterized in that the rotating body and the bracket can be separated or combined.
delete According to claim 1,
An idler is further included in the center of the second holder
A centrifugal separation device characterized in that the syringe and the sample processing unit are prevented from twisting.
According to claim 1,
A centrifugal separator, characterized in that it further comprises a power unit for transmitting rotational force to the rotating body.
According to claim 1,
The power unit is coupled to the connecting means vertically,
A centrifugal separation device characterized in that the sample processing part is gripped on the upper part of the connection means.
delete delete delete According to claim 1,
The centrifugal separator, characterized in that further comprising a filter in the inlet pipe and the outlet pipe.
According to claim 11,
The centrifugal separator, characterized in that the filter is detachable to the outflow pipe and the inlet pipe.
According to claim 11,
The filter is a centrifugal separator, characterized in that the hole is formed in units of 30 ~ 500um.

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