KR101457935B1 - Bottle for cell separation of fat and method using the same - Google Patents

Abstract

The present invention relates to a container for the cell separation of fat and a method for using the same. The container for the cell separation of fat according to the embodiment of the present invention comprises: a container body unit which includes an accommodating space formed therein and has a shape which can be mounted on a centrifuging device; a container inlet which is formed at a particular height at the top of the container body unit and is connected with the accommodating space; and a plurality of coupling projections which are formed to be separated from each other at particular intervals on the outer circumference of the container inlet. The container inlet has a shape on which a syringe hole of a syringe for feeding fat is mounted in order to be connected therewith, and the coupling projections are coupled with a coupling unit of the syringe for feeding fat. Moreover, the method for using the same according to the embodiment of the present invention comprises the steps of: extracting fat through a syringe for feeding fat and coupling a coupling unit of the syringe for feeding fat with a container for the cell separation of first fat, wherein a washing solution is filled in the container; mounting the container for the cell separation of first fat which is coupled with the syringe for feeding fat on a centrifuging device; and centrifuging fat and a washing solution by operating the centrifuging device.

Description

TECHNICAL FIELD The present invention relates to a container for separating fat cells,

The present invention relates to a container for cell separation of fat and a method of using the same. More particularly, the present invention relates to a liposuction device for preventing fat from being exposed to the air when the fat contained in a liposuction syringe is injected into a container from a liposuction syringe by attaching a container for separating fat cells to a liposuction syringe A container for cell separation, and a method for using a container for separating fat cells.

Fat transplantation is a procedure that is used to fill the necessary areas with the use of adipose tissue, and is generally used for enlargement molding such as face molding (nose, chin, ball, etc.) and breast. The fat used for the fat transplant is first taken through a harvesting step taken from the thigh or abdomen of the recipient of the fat transplant. Next, the collected fat is separated through cell separation into pure fatty tissue and oil and the like. Finally, the extracted fat tissue is extracted and subjected to an extraction step.

In the fat separating step, the fat is transferred to a container that is seated in a centrifuge used to separate the fat from the liposuction syringe used to collect the fat.

On the other hand, it is necessary to prevent as much as possible from exposure to air or contamination from other contaminants until the inhaled fat is again implanted in a specific site.

However, conventionally, in the process of transferring the fat from the syringe for liposuction to the container which is seated in the centrifuge, there is no separate blocking member for preventing the fat from being exposed to the air, so that the fat is exposed to the air, There is a problem in that the fat is contaminated.

In addition, consumables such as a pipette used for extracting fat in the step of extracting the separated fat are disposable or disinfected every time they are used, so that the cost for the fat transplantation procedure is excessively used or the fat transplantation procedure becomes complicated There was a problem.

Therefore, in order to prevent contamination of the inhaled fat by the external environment, the syringe, the centrifuge, and the fat extractor were treated with expensive equipment. However, such a device is too expensive, and there is a problem that the cost of consumables is also very high because the syringe, the tube, and the container dedicated to the equipment mounted on the equipment must be used and discarded once.

Korean Published Patent No. 10-2012-0069330 (June 28, 2012)

In order to solve the above-mentioned problems, the present invention can prevent the fat from being contaminated when the fat is transferred from the syringe for suctioning the fat to the container, reduce the cost used in the fat transplantation procedure, It is an object of the present invention to provide a container for cell separation of fat that can be simplified and a method of using the same.

The objects of the present invention are not limited to those mentioned above, and other objects not mentioned may be clearly understood by those skilled in the art from the following description.

According to an aspect of the present invention, there is provided a container for separating fat cells, comprising: a container body having a receiving space formed therein and having a shape that can be attached to a centrifuge; A container inlet formed at an upper portion of the container body and communicating with the accommodation space; And a plurality of engaging protrusions spaced apart from each other by a predetermined distance on the outer periphery of the container inlet portion, wherein the container inlet portion has a shape for being mounted on the injection port of the liposuction syringe and communicating with each other, And the protruding portion is coupled to the coupling portion of the liposuction syringe.

In addition, the height of each of the plurality of engaging projections may be reduced toward one direction on the outer circumference of the container inlet.

In addition, the container for separating the fat cells may further include a container cap covering the container inlet.

In addition, the container body portion may be formed in a conical shape whose diameter decreases toward the bottom.

In addition, the container for separating fat cells may further include a support for supporting the container body on the ground so as to be coupled to the lower portion of the container body.

According to an aspect of the present invention, there is provided a method of using a container for separating fat cells, comprising the steps of: collecting fat through a liposuction syringe; RTI ID = 0.0 > cell < / RTI > Attaching a container for cell separation of the first fat to which the liposuction syringe is attached to a centrifuge; And centrifuging the fat and the wash solution contained in the container for cell separation of the liposuction syringe and the first fat by operating the centrifuge.

In addition, after the step of centrifuging the fat and the washing solution contained in the container for cell separation of the liposuction syringe and the first fat, the syringe for liposuction and the container for cell separation of the first fat Removing the washing solution contained in the liposuction syringe; Binding a container for cell separation of the second fat filled with the enzyme solution to a coupling part of the liposuction syringe containing the centrifuged fat and mixing the enzyme solution and the fat to be centrifuged to prepare a mixed solution ; Attaching a container for cell separation of the second fat to which the liposuction syringe is attached to a centrifuge; And centrifugally separating the mixed solution contained in the container for separating the liposuction syringe and the second fat by operating the centrifugal separator.

Further, the method may further include extracting fat or fat tissue centrifuged from the mixed solution.

Further, the method may further include extracting cell sediment centrifuged from the fat or the mixed solution.

Also, the container for separating cells of the first fat includes a container body having a receiving space formed therein and having a shape capable of being attached to a centrifuge; A container inlet formed at an upper portion of the container body and communicating with the accommodation space; And a plurality of engaging protrusions spaced apart from each other by a predetermined distance on the outer periphery of the container inlet portion, wherein the container inlet portion is configured to be mounted on the injection port of the liposuction syringe and to communicate with each other, May be coupled to the coupling portion of the liposuction syringe.

In addition, the height of each of the plurality of engaging projections may be reduced toward one direction on the outer circumference of the container inlet.

The container for cell separation of the first fat may further include a container cap covering the container inlet.

In addition, the container body portion may be formed in a conical shape whose diameter decreases toward the bottom.

The container for separating cells of the first fat may further include a support for supporting the container body in the lower part of the container body so that the container body is erected on the ground.

According to the container for separating fat cells according to the present invention, the container for separating the cells of the fat is joined to the engaging portion of the syringe of the liposuction syringe by a plurality of engaging projections, thereby separating the fat cells from the syringe for liposuction It is possible to prevent the fat from being exposed to the air during the transfer of the fat. In addition, the present invention can prevent the fat from being exposed to the air during the centrifugal separation by centrifugally separating the fat contained in the syringe for liposuction in a state where a container for separating fat cells is attached to the syringe for liposuction. That is, the present invention can prevent the fat from being exposed to the air and being contaminated by the foreign substance by bonding the container for separating the fat cells to the liposuction syringe by a plurality of engaging projections.

According to the method of using a container for separating fat cells according to the present invention, the syringe containing the fat is centrifuged in a state of being combined with a container for separating the fat cells containing the washing solution or the enzyme solution, The centrifuged fat is formed into a plurality of layers in a container for cell syringe and fat cell separation and the fat tissue is transferred without using consumables such as a pipette used for transferring or extracting the centrifuged fat tissue to another container Can be extracted. That is, waste of consumables can be prevented by the method of using a container for separating cells of fat according to the present invention. Further, since the separated fat tissue can be transferred or extracted without using consumables such as a pipette or the like, it is not necessary to disinfect consumables at each step, so that the process of centrifuging fat can be simplified. Simplifying the process of centrifuging fat can simplify the process of fat transplantation using centrifuged fat.

The effects of the present invention are not limited to those mentioned above, and other effects not mentioned can be clearly understood by those skilled in the art from the following description.

1 is a perspective view showing a container for separating cells of fat according to an embodiment of the present invention.
FIG. 2 is an enlarged perspective view showing another side of the container inlet shown in FIG. 1. FIG.
3 is a perspective view showing a state before a container for separating fat cells is bonded to a liposuction syringe.
4 is a perspective view schematically showing a state where a container for separating fat cells is coupled to a liposuction syringe.
5 is a flowchart showing a procedure of a method of using a cell separation vessel according to an embodiment of the present invention.
FIG. 6 is a view showing the inside of a container for separating cells of a liposuction syringe and a first fat after centrifugation of the fat and washing solution contained in the container for separating the cells from the liposuction syringe and the first fat.
FIG. 7 is a view showing the inside of a container for separating a syringe for liposuction and a second fat after centrifugal separation of a mixed solution contained in a container for separating cells from a liposuction syringe and a second fat.

BRIEF DESCRIPTION OF THE DRAWINGS The above and other objects, features and advantages of the present invention will be more apparent from the following detailed description taken in conjunction with the accompanying drawings, in which: FIG. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unnecessary. The terms described below are defined in consideration of the structure, role and function of the present invention, and may be changed according to the intention of the user, the intention of the operator, or the custom.

The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. These embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art to which the present invention pertains, It is only defined by the scope of the claims. Therefore, the definition should be based on the contents throughout this specification.

Throughout the specification, when an element is referred to as being "comprising" or "comprising" an element, it is understood that the element may include other elements, not the exclusion of any other element .

Hereinafter, a container for separating fat cells and a method of using the same according to an embodiment of the present invention will be described in detail with reference to the accompanying drawings.

FIG. 1 is a perspective view showing a container 100 for separating fat cells according to an embodiment of the present invention.

Referring to FIG. 1, a container 100 for separating fat cells according to an embodiment of the present invention includes a container 100 having a receiving space 111 therein, a container 100 having a shape capable of being mounted on a centrifuge (not shown) A container inlet part 120 formed at a predetermined height above the container body part 110 and communicating with the accommodation space 111; And a plurality of coupling protrusions 130 spaced apart from each other.

The container body 110 may have a receiving space 111 formed therein. The container body 110 may contain a washing solution or an enzyme in a receiving space 111 formed therein. Alternatively, a cell precipitate separated from the fat after centrifugation of the fat, a predetermined amount of washing solution, or a predetermined amount of the enzyme solution may be accommodated in the receiving space 111. The upper portion of the container body portion 110 is connected to the container inlet portion 120 so that the accommodation space 111 can communicate with the container inlet portion 120. Accordingly, the fat sucked by the liposuction syringe, which will be described later, can be introduced into the receiving space 111 through the container inlet 120.

The container body 110 may be formed of a transparent or translucent material. The container body 110 is formed of a transparent or semitransparent material, so that the fat contained in the accommodation space 111 can be seen with the naked eye. Alternatively, the container body 110 is formed of a transparent or semitransparent material, so that the cell precipitate, a predetermined amount of the washing solution, or a predetermined amount of the enzyme solution described above can be visually confirmed.

The container body 110 has a shape that can be attached to the centrifuge. For example, the container body 110 may have a circular cross-section so as to be inserted into a tube insertion hole provided in the swing rotor of the centrifugal separator. However, the present invention is not limited to the above example, and may include all the structure and shape of the container body 110 for mounting the container body 110 to the centrifugal separator. Accordingly, the container body 110 can be easily mounted on the centrifuge.

The container body 110 may be formed to have a reduced diameter toward the bottom. That is, the lower portion of the container body 110 may be formed in a conical shape. Therefore, the container body 110 is mounted on the centrifugal separator and rotates at a swirling velocity of the fat, washing solution, or enzyme solution contained in the receiving space 111 toward the lower portion of the container body 110, Can be increased. If the swirling flow rate of the fat, washing solution or enzyme solution increases, the magnitude of the centrifugal force applied to the fat, washing solution or enzyme solution increases, and centrifugation of the fat, washing solution or enzyme can be facilitated.

The container 100 for separating fat cells may further include a support 150. The support portion 150 may be attached to a lower portion of the container body portion 110 formed in a conical shape. The supporting portion 150 may be formed in a triangular shape in its longitudinal section. That is, the overall shape of the lower portion of the container body 110 with the support portion 150 attached thereto may be formed in a rectangular shape when viewed from the front. Thus, the container body portion 110 can be erected on a flat surface by the support portion 150.

The container inlet part 120 may be formed in a columnar shape on the upper part of the container body part 110. For example, the container inlet portion 120 may be formed of a cylindrical or square column. However, it is not limited to the above example. Hereinafter, for convenience of explanation, the case where the container inlet 120 is formed in a columnar shape will be mainly described.

The container inlet 120 may have a cylindrical shape and may be formed at a predetermined height above the container body 110. One side of the container inlet 120 communicates with a receiving space 111 formed inside the container body 110. 3) of the liposuction syringe 200 (see FIG. 3) may be inserted at the other side of the container inlet 120, and more preferably, the injection hole 210 may be inserted into the container And can be closely inserted into the inner surface of the inlet 120. That is, the container inlet 120 may be formed in a shape that can communicate with the injection port 210 of the liposuction syringe 200. Therefore, the container inlet 120 can communicate with the liposuction syringe 200 by inserting a predetermined portion of the injection hole 210 into the other open side of the container inlet 120.

The plurality of engaging projections 130 may be formed on the outer periphery of the container inlet 120. Each of the plurality of engaging projections 130 may be spaced apart from each other by a predetermined distance on the outer circumference of the container inlet 120 formed in a cylindrical shape.

FIG. 2 is an enlarged perspective view of another side of the container inlet 120 shown in FIG. 1. FIG.

Referring to FIG. 2, each of the plurality of engaging protrusions 130 may decrease in height toward one direction on the outer circumference of the container inlet 120. For example, each of the plurality of engaging projections 130 has a height equal to the thread spacing of the engaging portion 220, and the height decreases from the one side to the counterclockwise direction, . ≪ / RTI > That is, each of the plurality of coupling protrusions 130 may be formed such that the height of the other side is smaller than the thread spacing of the coupling portion 220. Accordingly, the plurality of engaging projections 130 can be easily coupled to the threads formed in the engaging portion 220 of the liposuction syringe 200.

Hereinafter, how the plurality of coupling protrusions 130 are coupled to the coupling portion 220 of the liposuction syringe 200 will be described in more detail.

FIG. 3 is a perspective view showing a state before the container 100 for separating fat cells is joined to the liposuction syringe 200. 4 is a perspective view schematically showing a state in which the container 100 for separating fat cells is coupled to the syringe 200 for liposuction.

3 and 4, when the container body 110 is rotated counterclockwise, the plurality of engaging protrusions 130 are inserted into the thread of the engaging portion 220 while rotating counterclockwise together. As described above, each of the plurality of coupling protrusions 130 decreases in height in the counterclockwise direction and is formed to be smaller than the thread spacing of the coupling portion 220, so that each of the plurality of coupling protrusions 130 is engaged with the coupling portion 220). ≪ / RTI > Each of the plurality of coupling protrusions 130 is formed to have the same spacing as the thread of the coupling portion 220 so that when the plurality of coupling protrusions 130 are screwed to the coupling portion 220, It can be prevented from flowing inside. It is possible to prevent the coupling between the container body 110 and the liposuction syringe 200 from being loosened by preventing the plurality of coupling protrusions 130 from flowing inside the threads of the coupling part 220. [

That is, since the container 100 for separating fat cells is coupled to the liposuction syringe 200, a predetermined portion of the injection port 210 of the liposuction syringe 200 is inserted into the container inlet 120 The state can be maintained. Accordingly, the fat contained in the syringe 200 for liposuction is minimally exposed to the air during the introduction of the fat into the container 100 for separating the cells of the fat, thereby preventing contamination of the fat.

 The container 100 for separating fat cells may further include a container cap 140. The container closure 140 may cover the container inlet 120. The container opening portion 120 is covered by the container stopper 140 so that the receiving space 111 of the container body portion 110 can be sealed. At this time, the container stopper 140 may be provided with a sealing member therein. Therefore, the container stopper 140 can effectively block the inflow of the outside air into the container inlet 120. [ The present invention prevents the inflow of air into the receiving space 111 by the container stopper 140 so that the fat contained in the receiving space 111 can be kept clean.

Hereinafter, a method of using a container for separating fat cells according to an embodiment of the present invention will be described with reference to FIG. 5, which is a flowchart showing a procedure of using a container for separating fat cells according to an embodiment of the present invention . In this case, the container 100 for separating fat cells according to the embodiment of the present invention described above is used in the container using method for separating fat cells according to the embodiment of the present invention. Hereinafter, for convenience of explanation, a container 100 for separating fat cells used in a method for using a container for separating fat cells according to an embodiment of the present invention is provided. (Fig. 6) for cell separation of the first fat by a solution, and a vessel (100b, see Fig. 7) for cell separation of the second fat. 6) and a container 100b (see FIG. 7) for cell separation of the second fat, comprises a container 100 for cell separation of fat according to an embodiment of the present invention, The shape to be formed is the same.

5, the fat is collected through the syringe 200 for liposuction, and the container 100a for separating the first fat, which is filled with the washing solution in the coupling part 220 of the liposuction syringe 200, (S110). At this time, the fat contained in the liposuction syringe 200 may be in a state where the anesthetic solution is firstly removed. The amount of washing solution contained in the container 100a for cell separation of the first fat may be equal to the amount of fat removed from the anesthetic solution.

A container 100a for cell separation of the first fat, to which the liposuction syringe 200 is attached, is attached to the centrifuge (S120).

The centrifuge is operated to centrifuge the fat and washing solution contained in the syringe 200 for liposuction and the vessel 100a for cell separation of the first fat (S130). At this time, the centrifugation can be performed, for example, at a rotation speed of 1500 rpm for 5 minutes. However, the present invention is not limited to the above example, and the operator can select the optimum condition according to the purpose of the operation. The washing solution contained in the container 100a for separating the fat and the first fat contained in the syringe 200 through the centrifugal separation may be mixed and separated due to differences in specific gravity. At this time, the washing solution can remove the remaining anesthetic solution from the fat secondarily by mixing and separating the fat and the washing solution.

FIG. 6 is a schematic diagram of a syringe 200 for liposuction after centrifugation of the fat and washing solution contained in the syringe 200 for liposuction and the vessel 100a for cell separation of the first fat, And shows the inside of the container 100a.

6, the cleaning solution contained in the container 100a for separating the fat contained in the syringe 200 for liposuction and the first fat is stored in the syringe 200a according to the specific gravity of the substance contained in the fat and the cleaning solution, And a predetermined number of layers inside the container 200a and the container 100a for cell separation of the first fat. For example, a first oil 301, a first fat tissue 302, a cleaning solution 303 and a second oil 310 are disposed in the interior of the syringe 200 for liposuction and the container 100a for cell separation of the first fat, The first cell precipitate 304 may be separated into layers. That is, a part of the first oil 301, the first fat tissue 302, and the cleaning solution 303 may form a layer in the liposuction syringe 200, and a container for cell separation of the first fat A portion of the cleaning solution 303 and the first cell precipitate 304 may form a layer in the interior of the first cell 100a. However, the present invention is not limited to the above examples, and the number and types of layers to be separated may vary depending on the type of cleaning solution used and other kinds of additives.

Next, the container 100a for separating the first fat and the syringe 200 for liposuction are separated to separate the cell 100a for separating the first fat and the cleaning solution 200a contained in the liposuction syringe 200 (Step S140). At this time, some of the cleaning solution 303 may form a layer in the lower part of the liposuction syringe 200, and the rest may form a layer in the upper part of the container 100a for cell separation of the first fat.

When the cleaning solution 303 housed in the liposuction syringe 200 is removed, the first fat tissue 302 for fat transplantation is extracted. When the washing solution 303 stored in the container 100a for cell separation of the first fat is removed, the first cell precipitate 304 for fat transplantation is extracted. The extracted first adipose tissue 302 and the first cell precipitate 304 may be mixed and used for fat transplantation. Alternatively, the extracted first cell precipitate 304 may be mixed with fat extracted from a recipient of a fat transplant.

As described above, according to the present invention, since the washing solution 303 and the first cell precipitate 304 are separated and contained in the container 100a for cell separation of the first fat, The cleaning solution 303 or the first cell precipitate 304 accommodated in the container 100a for separation can be easily removed without using a consumable such as a pipette or the like. Therefore, since the consumables for extracting the cleaning solution 303 or the first cell precipitate 304 are minimized, waste of consumables can be prevented. In addition, since a consumable for extracting the cleaning solution 303 or the first cell precipitate 304 is not used, the process of disinfecting consumable products can be omitted, so that the process of centrifuging the fat can be simplified.

The present invention is characterized in that the vessel 100a for cell separation of the first fat and the syringe 200 for liposuction are separated to remove the washing solution 303 and then the first fat tissue 302 and the first cell precipitate (304) can be extracted. The first oil 301 and the first fat tissue 302 in the liposuction syringe 200 are enzymatically reacted and centrifuged to remove the first oil 301 and the first fat tissue 302 ) Is separated into a second fat tissue 402 (see FIG. 7), a second fat tissue 402 (see FIG. 7) and a second cell precipitate 404 The precipitate 404 can be extracted. Hereinafter, the process of extracting the second adipose tissue 402 and the second cell precipitate 404 after the enzymatic reaction will be described in detail.

When the washing solution 303 stored in the syringe 200 for liposuction is removed, the cell separation of the second fat, in which the enzyme solution is filled in the coupling portion 220 of the liposuction syringe 200 containing the fat, (See FIG. 7), and the enzyme solution and the centrifuged fat are mixed to produce a mixed solution (S150).

At this time, the centrifuged fat contained in the liposuction syringe 200 may be the first oil 301 and the first fat tissue 302. That is, the liposuction syringe 200 containing the first oil 301 and the first fat tissue 302 is combined with the container 100b for cell separation of the second fat filled with the enzyme solution, 301), the first adipose tissue 302 and the enzyme solution may be mixed to make a mixed solution. The enzyme solution may be formed of collagenase. However, the present invention is not limited to the above example, and an enzyme capable of having the same effect depending on the selection may be used. The amount of the enzyme solution contained in the container 100b for cell separation of the second fat may be equal to the amount of the first oil 301 and the first fat tissue 302 accommodated in the liposuction syringe 200. [

At this time, as the piston of the liposuction syringe 200 is moved in one direction, the first oil 301 and the first fat tissue 302 accommodated in the liposuction syringe 200 are separated from the second fat The first oil 301 and the first fat tissue 302, which are accommodated in the container 100b for cell separation of the second fat as the piston is moved in the other direction, Can be introduced into the liposuction syringe 200. The first oil 301 and the first fat tissue 302 are introduced into the interior of the syringe 200 for liposuction or the vessel 100b for cell separation of the second fat, 301), the first adipose tissue 302 and the enzyme solution may be mixed. The mixed solution may be prepared by mixing the first oil 301, the first fat tissue 302, and the enzyme solution by the above-described method, and the mixed solution may be prepared by mixing the liposuction syringe 200 and the second fat cells And can be accommodated in the container 100b for separation.

When the first oil 301, the first adipose tissue 302 and the enzyme solution are mixed, the first oil 301 and the first adipose tissue 302 can cause an enzyme reaction by the enzyme solution. At this time, the temperature at which the first oil 301 and the first adipose tissue 302 are enzymatically reacted may be 37 ° C. However, the present invention is not limited to the above example, and it may vary depending on conditions. In addition, the first oil 301, the first adipose tissue 302, and the enzyme solution may be mixed at intervals of 10 minutes as described above. However, the present invention is not limited to the above example, and the mixing time may vary depending on the change of the procedure condition.

The container 100b for separating the second fat into which the liposuction syringe 200 is bound is mounted on the centrifuge (S160).

The centrifugal separator is operated to centrifuge the mixed solution contained in the syringe 200 for liposuction and the container 100b for cell separation of the second fat (S170). At this time, the centrifugation can be performed at a rotation speed of 1500 rpm for 5 minutes. However, the present invention is not limited to the above-described example, and may vary depending on the procedure condition.

FIG. 7 is a view showing a syringe 200 for liposuction after centrifugal separation of the mixed solution contained in the syringe 200 for liposuction and the vessel 100b for cell separation of the second fat and a vessel for cell separation of the second fat 100b.

7, the mixed solution contained in the syringe 200 for liposuction and the container 100b for cell separation of the second fat is mixed with the first oil 301 mixed with the mixed solution, The syringe 200 may be divided into a predetermined number of layers in the interior of the syringe 200 and the container 100b for cell separation of the second fat according to the specific gravity of the enzyme solution and the enzyme solution. For example, the second oil 401, the second fat tissue 402, the enzyme solution 403, and the second fat tissue 403 are contained in the interior of the syringe 200 for liposuction and the container 100b for cell separation of the second fat, The second cell precipitate 404 can be separated into layers. That is, a portion of the second oil 401, the second fat tissue 402, and the enzyme solution 403 may form a layer in the liposuction syringe 200, and a container for cell separation of the second fat A part of the enzyme solution 403 and the second cell precipitate 404 may form a layer in the interior of the cell 200. However, the present invention is not limited to the above-described example, and it may vary depending on the procedure condition.

The second cellular precipitate 404 received in the container 200 for cell separation of the second fat is reacted with the first fatty tissue 302 by enzyme reaction with the enzyme solution 403, Such as adipocytes, lipid precursors, stem cells, etc., which are isolated from the cells.

Next, the container 100b for cell separation of the second fat and the syringe 200 for liposuction are separated, the enzyme solution 403 contained in the liposuction syringe 200 is removed, The extracted fat tissue is extracted (S180). At this time, the fat tissue may be the second fat tissue 402.

Next, the enzyme solution 403 contained in the container 100b for cell separation of the second fat is removed, and the cell suspension centrifuged from the mixed solution is extracted (S190). At this time, the cell precipitate may be the second cell precipitate 404.

The extracted second adipose tissue 402 and the second cell precipitate 404 may be mixed and used for fat transplantation. Alternatively, the extracted second cell precipitate 404 may be mixed with fat extracted from a recipient of a fat transplant.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

100: Container for the separation of fat cells
110:
120: container inlet portion
130: engaging projection
140: container cap
150: Support

Claims (14)

A container body having a receiving space formed therein and having a shape capable of being mounted on a centrifuge;
A container inlet formed at an upper portion of the container body and communicating with the accommodation space; And
And a plurality of engaging protrusions formed on the outer periphery of the container inlet part at a predetermined distance from each other,
Wherein the container inlet is mounted on a syringe of a liposuction syringe and has a shape for communicating with each other and the plurality of coupling protrusions are coupled to a coupling portion of the liposuction syringe
Container for fat cell separation.
The method according to claim 1,
Wherein each of the plurality of engaging projections has a height decreasing in a direction toward the outer periphery of the container inlet portion,
Container for fat cell separation.
The method according to claim 1,
The container for the cell separation of fat
Further comprising a container cap covering the container inlet portion,
Container for fat cell separation.
The method according to claim 1,
Wherein the container body portion is formed in a conical shape whose diameter decreases toward the bottom,
Container for fat cell separation.
5. The method of claim 4,
The container for the cell separation of fat
Further comprising: a support portion coupled to a lower portion of the container body portion to support the container body so as to stand on the ground surface,
Container for fat cell separation.
Collecting fat through a liposuction syringe and combining a vessel for cell separation of a first fat filled with a wash solution in a joint portion of the liposuction syringe;
Attaching a container for cell separation of the first fat to which the liposuction syringe is attached to a centrifuge; And
Centrifuging the fat and the wash solution contained in the container for cell separation of the first fat and the liposuction syringe by operating the centrifuge
Container utilization for fat cell separation.
The method according to claim 6,
After centrifuging the fat and the wash solution contained in the container for cell separation of the liposuction syringe and the first fat,
Separating the liposuction syringe and the container for cell separation of the first fat and removing the washing solution contained in the liposuction syringe;
Binding a container for cell separation of the second fat filled with the enzyme solution to a coupling part of the liposuction syringe containing the centrifuged fat and mixing the enzyme solution and the fat to be centrifuged to prepare a mixed solution ;
Attaching a container for cell separation of the second fat to which the liposuction syringe is attached to a centrifuge; And
Centrifuging the mixed solution contained in the container for cell separation of the liposuction syringe and the second fat by operating the centrifuge;
Container utilization for fat cell separation.
8. The method of claim 7,
Further comprising the step of extracting fat or fat tissue centrifuged from the mixed solution,
Container utilization for fat cell separation.
9. The method according to claim 7 or 8,
And extracting the cell sediment centrifuged from the fat or the mixed solution.
Container utilization for fat cell separation.
The method according to claim 6,
The container for cell separation of the first fat
A container body having a receiving space formed therein and having a shape capable of being mounted on a centrifuge;
A container inlet formed at an upper portion of the container body and communicating with the accommodation space; And
And a plurality of engaging protrusions formed on the outer periphery of the container inlet at a predetermined distance from each other,
Wherein the container inlet is mounted on a syringe of a liposuction syringe and has a shape for communicating with each other and the plurality of coupling protrusions are coupled to a coupling portion of the liposuction syringe
Container utilization for fat cell separation.
11. The method of claim 10,
Wherein each of the plurality of engaging projections has a height decreasing in a direction toward the outer periphery of the container inlet portion,
Container utilization for fat cell separation.
11. The method of claim 10,
The container for cell separation of the first fat
Further comprising a container cap covering the container inlet portion,
Container utilization for fat cell separation.
11. The method of claim 10,
Wherein the container body portion is formed in a conical shape whose diameter decreases toward the bottom,
Container utilization for fat cell separation.
14. The method of claim 13,
The container for cell separation of the first fat
Further comprising: a support portion coupled to a lower portion of the container body portion to support the container body so as to stand on the ground surface,
Container utilization for fat cell separation.

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