KR20190032798A - Centrifugal dehydrator - Google Patents

Abstract

A centrifugal separator of the present invention is a centrifugal separator for centrifugally separating a biological sample by rotating an injector comprising a syringe containing the obtained biological sample and a first holder and a second holder for sealing a front end and a distal end of the syringe. The centrifugal separator comprises: a rotating body being rotated; a bracket for interlocking with the rotating body and accommodating a part of the front end in a state in which the syringe is vertically installed and rotating the syringe about a long axis; and a sample processing unit for supporting a bottom of the syringe and sealing the inside of the syringe to supply fluid to the inside of the syringe and centrifugally separating an unnecessary sample from the biological sample.

Description

[0001] CENTRIFUGAL DEHYDRATOR [0002]

More particularly, the present invention relates to a centrifugal separator, and more particularly, to a centrifugal separator that rotates a syringe having a biological sample on its long axis axis to position a required sample on the inner wall of a syringe and to collect unnecessary samples on the center of the syringe, The present invention relates to a centrifugal separator for facilitating washing and exports of a sample.

Fat transplantation is not just the use of fat from fat as in the past, but the process of separating living fat cells through centrifugation and washing.

Unlike the oil from the destroyed fat cells and the blood from the inhalation cause the necrosis of the implanted adipose tissue, it is essential to improve the survival rate dramatically by introducing a technique for separating only living adipocytes.

In this procedure, a small incision is made in the body part, and a tube with a hole at the end (liposuction tube) is inserted, and the fat is sucked out by using the sound pressure of the syringe.

Then, liquid components such as adipose stem cells, red blood cells, blood, and water were separated into layers through the centrifugal separator in the syringe, and then the free oil, which was separated from the layered red blood cells and broken fat cells in the syringe free oil and the like, and only the adipose stem cells to be transplanted are taken and injected into the human body.

In order to achieve this, the fat syringe is transferred to a test tube for centrifugation at a positive pressure. Then, the test tube is attached to a centrifuge and rotated at a high speed to separate the fat substances according to the particle size and density, (Red blood cells, free oil).

Here, centrifugation utilizes the density difference between the solid and the liquid surrounding the solid. By putting some of the mixed material in the test tube and turning it quickly, it gives artificially strong acceleration, so that the heavy material sinks under the test tube and the light material floats on top.

That is, as shown in FIG. 1, as shown in FIG. 1, the inhaled fat is centrifuged, and erythrocytes E are located at the lowest layer in the test tube, free oil F is located at the uppermost layer, Adipose stem cell S is divided into layers.

Then, only the adipose stem cells S are extracted from the test tube through the syringe equipped with the needle.

However, the prior art has a problem that secondary infections due to the risk of microbial contamination may occur due to the exposure of the extracted fat to the outside air during the process of transferring the fat from the first syringe to the test tube.

In order to prevent the risk of such contamination, expensive equipment such as a clean bench or a clean room should be used. As a result, the apparatus and method for obtaining the adipose stem cell S are complicated and difficult.

In addition, when extracting only the adipose stem cell S in the test tube, there is a possibility that foreign substances may be inflowed and exposed to the outside air, causing a cause of secondary infections caused by air or foreign matter, and thus it is difficult to perform an aseptic extraction process .

This requires time and labor for the extraction of adipose stem cells S, which may lead to infection due to air contact and loss of pure fat.

Specifically, some medical procedures, such as fat transplantation and PRP concentration transplantation, started in the 1990s, using medical syringes as centrifuge containers to eliminate the inconvenience of transferring them to new centrifuge separation containers and minimizing contamination of airborne bacteria System is being utilized.

Syringe buckets which are disinfectionable and removable as a tool other than the necessary syringe are necessary for this purpose.

Because it is very uncomfortable and difficult to contaminate the gloves for disinfected gloves, some of the equipment that comes into contact with the healthcare personnel is pre-disinfected and sterilized by aseptic protocols before use Because it must be mounted.

In this case, since the long axis of the container is placed on the rotating surface or is placed at an angle, the flow of the fluid during rotation is not easily maintained, and the inside of the rotating container can not be observed. There is a problem that it is difficult to predict.

As shown in FIG. 2, when the platelets, leukocytes, stem cells, etc. in the blood are separated, it is impossible to observe the process of reducing the red blood cells by continuously passing the hemolyzed buffer solution that is 500 times or more than the blood amount. It was difficult to set the separation time and there was no actual application of this method.

Korean Patent Laid-Open Publication No. 2013-0062689, (2013.06.13) Korean Patent Laid-Open Publication No. 2015-0051026, (2015.05.11)

Accordingly, it is an object of the present invention to enable a centrifugal separator to centrifuge a syringe without removing the sample extracted from a human body through a syringe into a test tube.

It is another object of the present invention to provide a method for washing a biological sample by rotating a syringe having a biological sample on a long axis basis and positioning the required sample on the inner wall of the syringe and collecting the unnecessary sample in the center of the syringe, So that it can be easily accessed.

In order to attain the above object, the centrifugal separator of the present invention is characterized in that a syringe containing the obtained biological sample, a syringe composed of a first holder and a second holder each having a tip end and an end of the syringe are rotated, A centrifugal separator for separating a centrifugal separator, comprising: a rotating rotator; a bracket interlocked with the rotator and accommodating a tip portion in a state in which a syringe is vertically installed and rotating the syringe rotator about a long axis; And a sample processing unit for washing the unwanted sample in the biological sample after centrifugation by supplying the fluid to the inside in a state where the inside of the cylinder is sealed, and then taking out the sample to the outside.

According to the present invention, the bracket forms a multi-stepped groove which receives a part of the top end of the rectangular opening, wherein the multi-stepped groove has a first groove and a second groove formed concentrically with different diameters, 1 holder, and the second groove is configured to receive a portion of the ringer.

According to the present invention, the rotating body and the bracket can be separated or combined.

According to the present invention, the sample processing unit comprises cleaning means for supplying the cleaning solution through the inlet pipe coupled to the inside of the syringe, and removing means for sucking the cleaning solution and the unnecessary sample through the outlet pipe connected to the inside of the syringe.

According to the present invention, idling means is further provided at the center of the second holder to prevent twisting of the syringe and the sample processing unit.

According to the present invention, the power unit further transmits rotational force to the rotating body.

According to the present invention, the power section is vertically connected to the connecting means, and the upper portion of the connecting means grips the sample treating section.

According to the present invention, the second groove further includes an elastic material pressing member on the inner circumference, so that the ring is elastically fitted in the second groove.

According to the present invention, the cleaning means further includes a cleaning liquid supply means for pressure-feeding the cleaning water, and the first conduit connected to the cleaning liquid supply means is connected to the inlet pipe.

According to the present invention, the removing means further includes a purging means for generating a vacuum pressure for taking out the unnecessary sample and the washing water in the syringe to the outside, and a second pipe connected to the purging means is connected to the outlet pipe .

According to the present invention, the inlet pipe and the outlet pipe further include a filter.

According to the present invention, the filter can be detachably attached to the outflow pipe and the inflow pipe.

According to the present invention, the filter is formed with holes in the unit of 30 to 500 um.

As described above, in the centrifugal separator for a syringe according to the present invention, a syringe containing the obtained biological sample, a syringe composed of a first holder and a second holder each having a tip end and an end of the syringe are rotated to centrifuge the biological sample The centrifugal separator includes: a rotating body; a bracket interlocked with the rotating body and accommodating a part of the front end in a state in which the syringe is vertically installed, for rotating the syringe about its long axis; And a sample processing unit for washing the unnecessary sample in the biological sample after the centrifugal separation by supplying the fluid to the inside in a state where the inside of the cylinder is sealed, and then taking out the sample to the outside.

Thus, it is possible to centrifuge the syringe by attaching the syringe to the centrifuge without moving the sample extracted from the human body through the syringe to the test tube.

In addition, the syringe having the biological sample is rotated with respect to the major axis, and the required sample is positioned on the inner wall of the syringe, and the unnecessary sample is divided so as to be collected at the center of the syringe. have.

1 is a perspective view showing a syringe for a sample applied to centrifugal separation;
2 is a perspective view of a centrifugal separator according to the prior art.
3 is a perspective view showing a state in which a centrifuge is attached to a centrifugal separator according to the prior art.
4 is a front sectional view showing a centrifugal separator of the present invention.
5 is a front sectional view showing a centrifugal separation step using the centrifugal separator of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

First, in the drawings, it is noted that the same components or parts are denoted by the same reference numerals as possible. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted so as to avoid obscuring the subject matter of the present invention.

FIG. 4 is a front sectional view showing a centrifugal separator of the present invention, and FIG. 5 is a front sectional view showing a centrifugal separating step using the centrifugal separator of the present invention.

4 and 5, in the centrifugal separator 10 of the present invention, a syringe 100 containing a biological sample is vertically installed in the centrifugal separator 10, the centrifugal separator 10 is rotated about the long axis to centrifuge the biological sample, The sample is taken out and the inside of the syringe 100 is cleaned.

Here, the syringe 100 combines the syringe 110 containing the obtained biological sample, the first holder 120 and the second holder 130, which are respectively connected to the front end and the end of the syringe 110, respectively.

And seals the space of the cylinder 110 through the first holder 120 and the second holder 130.

The configuration of an apparatus for centrifuging the syringe 100 is as follows.

The centrifugal separator 10 includes a power unit 500, a rotating body 200, a bracket 300, and a sample processing unit 400.

The power unit 500 includes a motor for generating a rotational force, and a rotational axis of the motor is vertically positioned on the top.

Also, the rotating body 200 is coupled to the rotating shaft, and the rotating body 200 rotates through the rotating force of the power unit 500.

The bracket 300 is further coupled to the upper portion of the rotating body 200.

The bracket 300 interlocks with the rotating body 200 and rotates the syringe 110 about the long axis by receiving a part of the tip with the syringe 110 vertically installed.

The multi-stepped groove 310 has a first groove 311 and a second groove 312 concentrically formed with different diameters, so that the first groove 311 And the second groove 312 is adapted to receive a portion of the ring 110.

That is, the first groove 311 and the second groove 312 are continuously formed in multiple stages so that the outer shape of the syringe 100 of the bracket 300 can be accommodated without interference, so that the first holder 120 and the outer periphery of the ring- Respectively.

Here, the rotating body 200 and the bracket 300 can be separated or combined. This is applied when performing the regular disinfection of the bracket 300.

At this time, in order to prevent the syringe 100 coupled to the bracket 300 from being disengaged, the second groove 312 is provided with a pressing member 313 made of an elastic material on the inner circumference thereof.

The pressing member 313 may be made of elastic rubber so that the ring finger 110 is elastically fitted into the second groove 312 through the pressing member 313. [

In addition, the sample processing unit 400 supports the rotation of the syringe 110 and supplies the fluid to the inside of the syringe 110 in a sealed state. After centrifugation, the unnecessary sample in the biological sample is washed at the syringe 110 and taken out to the outside.

The sample processing unit 400 includes a washing unit 410 for supplying a washing solution through an inflow pipe 413 coupled to the inside of the syringe 110 and a removing unit 420 for sucking a washing solution and an unnecessary sample through an outflow pipe 423 connected to the inside of the syringe 110 do.

Here, the cleaning means 410 further includes a cleaning liquid supply means 411 for pressure-feeding the cleaning liquid, and the first conduit 412 connected to the cleaning liquid supply means 411 is connected to the inlet pipe 413.

The removing means 420 further includes a purging means 421 for generating a vacuum pressure for taking out unnecessary samples and washing water in the syringe 110 to the outside, and a second pipe 422 connected to the purging means 421 and an outlet pipe 423 Lt; / RTI >

 After the centrifugation, the unnecessary sample except the necessary sample adhered to the inner wall of the syringe 110 is filled in the syringe 110, and the washing solution is supplied into the syringe 110 through the inflow pipe 413 to lower the point of the unnecessary sample The unneeded sample diluted in the washing solution is sucked into the vacuum pressure through the outlet pipe 423 and is taken out to the outside.

The filter 440 couples the filter 440 to the outflow pipe 423 and the inflow pipe 413 so as to be detachable.

The outflow pipe 423 and the inflow pipe 413 have a maximum length of 50 cm or less in consideration of the general size of a medical high-pressure steam sterilizer or an EO gas sterilizer, a plasma sterilizer, and a self-sterilizer.

Particularly, the outflow pipe 423 and the inflow pipe 413 are made of polymer (PE, PP, PC, Teflon, silicone, ABS, MA, acetal, acrylic, polystyrene or the like) which is stable to heat of 80 degrees or more and does not react with sterilizing gas, It is made of metal only.

Here, the filter 440 is formed with holes of 30 to 500 um.

The second holder 130 further includes an idling means 430 at the center thereof to prevent the slinger 110 and the sample processing unit 400 from being twisted.

Here, the idling means 430 is applicable as a bearing, and is idly rotated in the second holder 130 interlocked with the ring heater 110 to prevent the outflow pipe 423 and the inflow pipe 413 from being twisted.

The centrifugal separator 10 having the above-described structure rotates the long shaft of the transparent cylindrical syringe 100 through the centrifugal separation step to rotate on the rotation axis so that the unnecessary sample is collected on the inner wall of the syringe holder 110 and the thickness of the layer can be visually observed. It is possible to easily form a fluid flow path in the center and to perform a process such as continuous washing.

A centrifugal separation method using the centrifugal separator 10 having the above configuration is as follows.

A representative target procedure for centrifugation is the Stromal Vascular Fraction (SVF) or Platelet-Rich Plasma (PRP).

First, when the stromal stem cell fractionation procedure is performed using the centrifugal separator 10 of the present invention, the sample syringe 100 is used for sampling in the process of dissolving the human tissue in the proteolytic enzyme and then separating only the cells, By using the method used as an enzyme reaction vessel as it is, the probability of contamination is lowered.

However, it is still a problem that the process of final washing of enzyme is complicated and takes a long time for washing. It is a problem that already separated cells attach to untreated tissues during washing time, .

Therefore, it is possible to simplify the cleaning process and minimize the cleaning time, and to easily reprocess the non-decomposed tissue.

When platelet-rich plasma is performed using the centrifugal separator 10 of the present invention, the blood contained in the syringe 100 is transferred to a special container, centrifuged, and then the heaviest red blood cells are collected in the lower part, the light serum is collected in the upper part, , Stem cells, and platelets exist between them.

In this method, it is difficult to remove only the platelet layer formed by the buffy coat with many platelets buried together with red blood cells during centrifugation.

If you can remove only serum and red blood cells from your blood, you can get platelets and leukocyte stem cells together. To test a small amount of sample, use red blood cell hemolysis hypotonic buffer to remove red blood cells.

The problem with this erythrocyte removal method is that it requires about 500 cc of buffer wash for 1 cc of blood and usually requires 20,000 cc of wash solution because it uses more than 20 cc of blood and it takes a lot of time to concentrate other cells from this amount of wash solution It is necessary.

Therefore, if a tube long axis centrifugal separator for washing and collecting a large amount of sludge is applied through the sample treatment unit 400 of the present invention, the erythrocyte lysis method is effective for collecting other blood cells.

The technical features of the centrifugal separator 10 constructed as described above are as follows.

 First, the transparent medical syringe 100 sterilized through a centrifuge is allowed to rotate in the long axis.

As a result, the syringe 100, which is a necessary disposable container in the aseptic process, can be easily applied to the centrifugal separator 10, which facilitates separation and coupling.

Eventually, an independent medical syringe 100 is used as part of a centrifuge without the need to move the container.

In addition, the outflow pipe 423 and the inflow pipe 413 are prevented from being twisted in a state in which rotational friction is reduced through the idle rotating means 430.

Even if the washing liquid is continuously flowed through the inflow pipe 413, the biological sample contained in the syringe 100 is not adhered to the inner wall of the syringe 100 because it is spread and fixed. Therefore, it is easy and time-consuming to wash a large amount of biological sample or to dilute impurities.

Especially. Since the required sample to be collected is attached to the wall of the syringe 100, the unnecessary sample is collected at the central portion except the inner wall of the syringe 110. Therefore, it is easy to remove the impurities because the supernatant can be removed from the inside when centrifuged.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the inventions. It will be clear to those who have knowledge of.

10: Centrifuge 100: Syringe
110: ringer 120: first holder
130: second holder 200: rotating body
300: Bracket 310: Multi-step groove
311: first groove 312: second groove
313: pressing member 400: sample processing unit
410: Cleaning means 411: Cleaning liquid supply means
412: first conduit 413: inlet pipe
420: removing means 421:
422: second conduit 423: outlet pipe
430 idling means 440 filter
500: power unit 510: connecting means

Claims (13)

A centrifugal separator for centrifuging a living body sample by rotating a syringe containing the obtained biological sample, a syringe made up of a first holder and a second holder each having a tip end and a tip end of the syringe,
A rotating body,
A bracket for interlocking with the rotating body and accommodating a part of the tip in a state in which the syringe is vertically installed and rotating the syringe about the long axis;
And a sample processing unit for supporting the syringe bottom and sealing the inside of the syringe and supplying the fluid to the inside of the syringe to clean unnecessary sample in the syringe and centrifuge the sample to be taken out to the outside after centrifugal separation. Device.
The method according to claim 1,
The bracket
The upper end of which is formed with a multi-step groove for partially accommodating the tip end of the opening,
The multi-step groove is formed with a first groove and a second groove concentric with each other in diameter
Wherein the first groove is configured to receive the first holder and the second groove to receive the portion of the caliper.
The method according to claim 1,
Wherein the rotating body and the bracket can be separated or combined.
The method according to claim 1,
The sample processing unit includes:
Cleaning means for supplying the cleaning solution through the inflow pipe coupled to the inside of the syringe,
And a removing means for sucking the washing solution and the unnecessary sample through an outflow pipe connected to the inside of the syringe.
The method according to claim 1,
Further comprising idling means at the center of the second holder
Thereby preventing the syringe and the sample processing unit from being twisted.
The method according to claim 1,
Wherein the centrifugal separator further comprises a power unit for transmitting a rotational force to the rotating body.
The method according to claim 1,
The power unit is vertically connected to the connecting means,
And the sample processing unit is held on the upper portion of the connecting means.
The method according to claim 1,
The second groove may further include a pressing member made of an elastic material at an inner periphery thereof
And the syringe is elastically fitted in the second groove.
5. The method of claim 4,
The cleaning means,
Further comprising washing liquid supply means for feeding the washing water,
Wherein the first conduit connected to the cleaning liquid supply means is connected to the inlet pipe.
5. The method of claim 4,
Wherein the removing means comprises:
Further comprising purging means for generating a vacuum pressure for taking out the unnecessary sample and washing water in the syringe to the outside,
And an outlet pipe is connected to a second pipe connected to the purging means.
5. The method of claim 4,
Wherein the inlet pipe and the outlet pipe further include a filter.
12. The method of claim 11,
Wherein the filter is detachably attachable to the outflow pipe and the inflow pipe.
12. The method of claim 11,
Wherein the filter has holes of 30 to 500 um.

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