KR102486713B1 - 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법 - Google Patents

재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법 Download PDF

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KR102486713B1
KR102486713B1 KR1020197029638A KR20197029638A KR102486713B1 KR 102486713 B1 KR102486713 B1 KR 102486713B1 KR 1020197029638 A KR1020197029638 A KR 1020197029638A KR 20197029638 A KR20197029638 A KR 20197029638A KR 102486713 B1 KR102486713 B1 KR 102486713B1
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조슈아 키틀슨
토마스 스티븐슨
레나 힐
카를로스 구스타보 페세
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볼트 쓰레즈, 인크.
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

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  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Insects & Arthropods (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
KR1020197029638A 2017-03-10 2018-03-09 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법 Active KR102486713B1 (ko)

Priority Applications (1)

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KR1020237000420A KR102618002B1 (ko) 2017-03-10 2018-03-09 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법

Applications Claiming Priority (3)

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US201762470153P 2017-03-10 2017-03-10
US62/470,153 2017-03-10
PCT/US2018/021817 WO2018165594A1 (en) 2017-03-10 2018-03-09 Compositions and methods for producing high secreted yields of recombinant proteins

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KR1020237000420A Division KR102618002B1 (ko) 2017-03-10 2018-03-09 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법

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KR20190127806A KR20190127806A (ko) 2019-11-13
KR102486713B1 true KR102486713B1 (ko) 2023-01-10

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KR1020197029638A Active KR102486713B1 (ko) 2017-03-10 2018-03-09 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법
KR1020237000420A Active KR102618002B1 (ko) 2017-03-10 2018-03-09 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법
KR1020257025185A Pending KR20250121449A (ko) 2017-03-10 2018-03-09 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법
KR1020237044072A Active KR102840788B1 (ko) 2017-03-10 2018-03-09 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법

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KR1020257025185A Pending KR20250121449A (ko) 2017-03-10 2018-03-09 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법
KR1020237044072A Active KR102840788B1 (ko) 2017-03-10 2018-03-09 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법

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US (4) US10906947B2 (https=)
EP (1) EP3592762A4 (https=)
JP (3) JP7063486B2 (https=)
KR (4) KR102486713B1 (https=)
WO (1) WO2018165594A1 (https=)

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US12351805B2 (en) 2015-11-19 2025-07-08 Northeastern University Method of making recombinant silk and silk-amyloid hybrid proteins using bacteria
WO2017087827A1 (en) * 2015-11-19 2017-05-26 President And Fellows Of Harvard College Method of making recombinant silk andsilk-amyloid hybrid proteins using bacteria
EP3592800A4 (en) 2017-03-10 2021-01-06 Bolt Threads, Inc. COMPOSITIONS AND METHODS OF PRODUCING HIGHLY SECRETED YIELD OF RECOMBINANT PROTEIN
KR102486713B1 (ko) 2017-03-10 2023-01-10 볼트 쓰레즈, 인크. 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법
EP4048787A4 (en) * 2019-11-27 2023-12-13 Revelations Biotech Pvt Ltd Nucleic acids, vectors, host cells and methods for production of fructosyltransferase from aspergillus japonicus
US20240141363A1 (en) 2021-02-12 2024-05-02 Boehringer Ingelheim Rcv Gmbh & Co Kg Signal peptides for increased protein secretion
US11993068B2 (en) 2022-04-15 2024-05-28 Spora Cayman Holdings Limited Mycotextiles including activated scaffolds and nano-particle cross-linkers and methods of making them
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2024141641A2 (en) 2022-12-30 2024-07-04 Biotalys NV Secretion signals
US12467171B2 (en) 2023-10-13 2025-11-11 Spora Cayman Holdings Limited Large-scale production of mycelium-based textiles at mushroom farm facilities
WO2026027251A1 (en) 2024-08-01 2026-02-05 Boehringer Ingelheim Rcv Gmbh & Co Kg Signal peptides for increased protein secretion

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Also Published As

Publication number Publication date
US20180282381A1 (en) 2018-10-04
JP7063486B2 (ja) 2022-05-09
KR20250121449A (ko) 2025-08-12
US20220306706A1 (en) 2022-09-29
US20210230235A1 (en) 2021-07-29
JP2020509755A (ja) 2020-04-02
KR20240005134A (ko) 2024-01-11
KR20190127806A (ko) 2019-11-13
US12122810B2 (en) 2024-10-22
US11370815B2 (en) 2022-06-28
KR102618002B1 (ko) 2023-12-27
EP3592762A1 (en) 2020-01-15
US10906947B2 (en) 2021-02-02
EP3592762A4 (en) 2020-12-30
US20250122252A1 (en) 2025-04-17
WO2018165594A1 (en) 2018-09-13
KR20230011481A (ko) 2023-01-20
KR102840788B1 (ko) 2025-07-31
JP2022078220A (ja) 2022-05-24
JP2024045697A (ja) 2024-04-02

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