KR102481661B1 - A cosmetic composition effective for skin barrier improvement and moisturizing containing vegan exosome, vegan peptide, vegan hyaluronic acid and vegan ceramide - Google Patents
A cosmetic composition effective for skin barrier improvement and moisturizing containing vegan exosome, vegan peptide, vegan hyaluronic acid and vegan ceramide Download PDFInfo
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- KR102481661B1 KR102481661B1 KR1020220136229A KR20220136229A KR102481661B1 KR 102481661 B1 KR102481661 B1 KR 102481661B1 KR 1020220136229 A KR1020220136229 A KR 1020220136229A KR 20220136229 A KR20220136229 A KR 20220136229A KR 102481661 B1 KR102481661 B1 KR 102481661B1
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
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Abstract
Description
본 발명은 비건엑소좀, 비건펩타이드, 비건히알루론산 및 비건세라마이드를 함유한 피부 장벽개선, 보습에 효과적인 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition effective for skin barrier improvement and moisturizing containing vegan exosomes, vegan peptides, vegan hyaluronic acid and vegan ceramide.
피부는 외부 환경의 물리적, 화학적 자극으로부터 인체를 보호하는 동시에 수분 유지 및 체온 유지에 중요한 역할을 한다. 피부는 외부에서부터 표피, 진피, 피하 조직의 3개 층으로 구분되며, 표피는 외부로부터 각질층, 과립층, 유극층, 기저층의 순서로 구분된다. 각질층은 피부의 가장 바깥쪽에 위치하면서, 케라틴 단백질 약 58%, 천연보습인자(natural moisturizing factor, NMF) 약 38%, 지질 약 11%로 구성되어 있다. 천연보습인자는 각질층의 수분을 붙잡는 역할을 한다. 각질층은 단백질이 풍부한 각질 세포와 세라마이드(ceramide), 자유 지방산, 콜레스테롤과 같은 다양한 지질로 이루어진 각질세포 간 지질로 이루어져 있으며, 각질세포와 그 사이의 지질로 피부 장벽을 구성하게 된다.The skin protects the human body from physical and chemical stimuli in the external environment and plays an important role in maintaining moisture and maintaining body temperature. The skin is divided into three layers of the epidermis, dermis, and subcutaneous tissue from the outside, and the epidermis is divided into the stratum corneum, the granular layer, the spinous layer, and the basal layer from the outside in order. The stratum corneum is located at the outermost layer of the skin and is composed of keratin protein (58%), natural moisturizing factor (NMF) (38%), and lipid (11%). Natural moisturizing factor holds the moisture in the stratum corneum. The stratum corneum consists of protein-rich keratinocytes and lipids between keratinocytes composed of various lipids such as ceramide, free fatty acids, and cholesterol, and constitutes a skin barrier with keratinocytes and lipids between them.
정상적인 각질세포의 수분이 10 내지 30% 정도로 유지되면 피부가 매끄럽고 부드러운 상태를 유지하는데, 각질세포의 수분함량이 10% 이하가 되면 피부가 거칠어지고 피부 노화가 발생하며 각질세포가 벗겨져 나오는 스케일링(scaling) 현상이 발생하게 된다. 특히, 대부분의 초기 피부 노화는 피부의 건성화로인한 피부 탄력성 저하와 주름 발생으로부터 시작된다.When the moisture of normal keratinocytes is maintained at 10 to 30%, the skin maintains a smooth and soft state. ) phenomenon occurs. In particular, most early skin aging begins with a decrease in skin elasticity and wrinkles due to dryness of the skin.
아미노산, 당류와 같은 천연보습인자는 피부가 유연성을 나타내도록 작용함은 물론 적절한 수분을 유지할 수 있도록 돕는다. 특히, 아미노산과 같은 물질은 그 자체로 수용성일 뿐만 아니라 효과적으로 수분과 결합하여 피부에서 수분이 건조되는 것을 억제할 수 있으나, 이들은 피부에 자극을 유발하거나 제형 안정성 등에 문제가 있다.Natural moisturizing factors such as amino acids and sugars help the skin to show flexibility and maintain proper moisture. In particular, substances such as amino acids are not only water-soluble in themselves, but also can effectively combine with moisture to suppress drying of moisture from the skin, but they cause irritation to the skin or have problems with formulation stability.
이에, 인체에 무해하면서 피부와의 친화력이 높아 민감한 피부에도 자극 없이, 피부 보습 효과에 탁월한 효과를 발휘하는 피부 보습제의 개발 필요성은 여전하다.Therefore, there is still a need to develop a skin moisturizer that is harmless to the human body and has a high affinity for the skin, so that it does not irritate sensitive skin and exerts an excellent effect on skin moisturizing.
본 발명은 상기와 같은 문제점을 해결하기 위해 안출된 것으로서, 본 발명의 목적은 피부 장벽개선, 보습에 효과적인 화장료 조성물을 제공하는 것이다.The present invention has been made to solve the above problems, and an object of the present invention is to provide a cosmetic composition effective for skin barrier improvement and moisturizing.
본 발명의 과제는 이상에서 언급한 과제들로 제한되지 않으며, 언급되지 않은 또 다른 과제들은 아래의 기재로부터 당업자에게 명확하게 이해될 수 있을 것이다.The object of the present invention is not limited to the tasks mentioned above, and other tasks not mentioned will be clearly understood by those skilled in the art from the following description.
상기 목적을 달성하기 위하여 본 발명은In order to achieve the above object, the present invention
수삼 유래 엑소좀, 아세틸헥사펩타이드-8, 올리고 히알루론산, 피토콜라겐 및 세라마이드를 포함하는 화장료 조성물을 제공한다.Provided is a cosmetic composition containing fresh ginseng-derived exosomes, acetylhexapeptide-8, oligo hyaluronic acid, phyto-collagen and ceramide.
또한, 상기 화장료 조성물은 수삼 유래 엑소좀 1-5 중량부, 소자 유래 엑소좀 1-5 중량부, 아세틸헥사펩타이드-8 0.5-4 중량부, 올리고 히알루론산 4-8 중량부, 피토콜라겐 1-5 중량부, 세라마이드 0.5-4 중량부, 소자 추출물 0.3-2 중량부, 시계초 추출물 0.3-2 중량부, 오미자씨 오일 0.3-2 중량부, 복숭아씨 오일 0.3-2 중량부, 호박씨 발효추출물 0.3-2 중량부, 은행나무잎 추출물 0.3-2 중량부, 아보카도 추출물 0.3-2 중량부, 글리세린 1-5 중량부, 스쿠알란 0.5-4 중량부, 펜틸렌글라이콜 0.5-4 중량부, 나이아신아마이드 0.1-1 중량부, 폴리글리세릴-6스테아레이트 0.1-1 중량부, 폴리글리세릴-2스테아레이트 0.1-1 중량부, 부틸렌글라이콜 2-6 중량부, 세테아릴알코올 0.5-4 중량부, 글리세릴스테아레이트 0.5-4 중량부, 스테아릴알코올 0.1-1 중량부, 프로판다이올 0.5-4 중량부, 카보머 3-7 중량부, 세틸에틸헥사노에이트 0.5-4 중량부, 솔비톨 0.1-1 중량부, 트로메타민 0.1-1 중량부, 폴리글리세릴-6베헤네이트 0.1-1 중량부, 1,2-헥산다이올 0.1-1 중량부, 리모넨 0.5-4 중량부, 리날룰 0.5-4 중량부 및 당근수 38-42 중량부를 포함하고,In addition, the cosmetic composition contains 1-5 parts by weight of fresh ginseng-derived exosomes, 1-5 parts by weight of soybean-derived exosomes, 0.5-4 parts by weight of acetyl hexapeptide-8, 4-8 parts by weight of oligo hyaluronic acid, phytocollagen 1- 5 parts by weight, ceramide 0.5-4 parts by weight, soja extract 0.3-2 parts by weight, passion fruit extract 0.3-2 parts by weight, Schizandra seed oil 0.3-2 parts by weight, peach seed oil 0.3-2 parts by weight, pumpkin seed fermented extract 0.3 parts by weight -2 parts by weight, ginkgo leaf extract 0.3-2 parts by weight, avocado extract 0.3-2 parts by weight, glycerin 1-5 parts by weight, squalane 0.5-4 parts by weight, pentylene glycol 0.5-4 parts by weight, niacinamide 0.1 -1 part by weight, 0.1-1 part by weight of polyglyceryl-6 stearate, 0.1-1 part by weight of polyglyceryl-2 stearate, 2-6 parts by weight of butylene glycol, 0.5-4 part by weight of cetearyl alcohol 0.5-4 parts by weight of glyceryl stearate, 0.1-1 part by weight of stearyl alcohol, 0.5-4 parts by weight of propanediol, 3-7 parts by weight of carbomer, 0.5-4 parts by weight of cetylethylhexanoate, 0.1-4 parts by weight of sorbitol -1 part by weight, 0.1-1 part by weight of tromethamine, 0.1-1 part by weight of polyglyceryl-6behenate, 0.1-1 part by weight of 1,2-hexanediol, 0.5-4 part by weight of limonene, 0.5 part by weight of linalool -4 parts by weight and 38-42 parts by weight of carrots,
상기 올리고 히알루론산은 중량평균분자량이 1,000-3,000인 히알루론산 63-67 중량부 및 중량평균분자량이 15,000-20,000인 히알루론산 33-37 중량부를 포함하는 것을 특징으로 한다.The oligo hyaluronic acid is characterized by comprising 63-67 parts by weight of hyaluronic acid having a weight average molecular weight of 1,000-3,000 and 33-37 parts by weight of hyaluronic acid having a weight average molecular weight of 15,000-20,000.
또한, 본 발명은In addition, the present invention
수삼을 세척하여 18-22℃의 온도에서 140-160 MPa의 압력으로 3-7분 동안 전처리하는 단계; 전처리된 수삼을 착즙하여 수삼 착즙액을 제조하는 단계; 상기 수삼 착즙액을 원심분리하여 엑소좀을 포함하는 상층액을 수득하는 단계; 상기 엑소좀을 포함하는 상층액을 동결건조하여 동결건조물을 제조하는 단계; 상기 동결건조물에 폴리에틸렌글리콜/덱스트란을 사용하여 수성 2상계를 형성하는 단계; 및 상기 수성 2상계 내 엑소좀이 농축된 하층액을 수득하는 단계;를 포함하는 수삼 유래 엑소좀을 제조하는 단계;Washing fresh ginseng and pre-treating it at a temperature of 18-22°C and a pressure of 140-160 MPa for 3-7 minutes; Preparing fresh ginseng juice by extracting pretreated fresh ginseng; Obtaining a supernatant containing exosomes by centrifuging the fresh ginseng juice; preparing a lyophilisate by freeze-drying the supernatant containing the exosomes; forming an aqueous two-phase system using polyethylene glycol/dextran in the lyophilisate; and obtaining a lower layer in which exosomes are concentrated in the aqueous two-phase system; preparing fresh ginseng-derived exosomes;
소자를 세척하여 28-32℃의 온도에서 290-310 MPa의 압력으로 1-5분 동안 전처리하는 단계; 전처리된 소자를 착즙하여 소자 착즙액을 제조하는 단계; 상기 소자 착즙액을 원심분리하여 엑소좀을 포함하는 상층액을 수득하는 단계; 상기 엑소좀을 포함하는 상층액을 동결건조하여 동결건조물을 제조하는 단계; 상기 동결건조물에 폴리에틸렌글리콜/덱스트란을 사용하여 수성 2상계를 형성하는 단계; 및 상기 수성 2상계 내 엑소좀이 농축된 하층액을 수득하는 단계;를 포함하는 소자 유래 엑소좀을 제조하는 단계;washing the device and pre-treating it at a temperature of 28-32° C. and a pressure of 290-310 MPa for 1-5 minutes; Preparing a device juice by extracting the pretreated device; Obtaining a supernatant containing exosomes by centrifuging the juice from the cow; preparing a lyophilisate by freeze-drying the supernatant containing the exosomes; forming an aqueous two-phase system using polyethylene glycol/dextran in the lyophilisate; and obtaining an exosome-enriched lower layer in the aqueous two-phase system; preparing a device-derived exosome comprising;
중량평균분자량이 1,000-3,000인 히알루론산 63-67 중량부 및 중량평균분자량이 15,000-20,000인 히알루론산 33-37 중량부를 혼합하여 혼합물을 제조하는 단계; 및 상기 혼합물을 33-37℃의 온도에서 2-6시간 동안 교반하는 단계;를 포함하는 올리고 히알루론산을 제조하는 단계;Preparing a mixture by mixing 63-67 parts by weight of hyaluronic acid having a weight average molecular weight of 1,000-3,000 and 33-37 parts by weight of hyaluronic acid having a weight average molecular weight of 15,000-20,000; and stirring the mixture at a temperature of 33-37° C. for 2-6 hours; preparing an oligo hyaluronic acid comprising;
소자 및 시계초를 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 소자분말 및 시계초분말을 준비하는 단계; 상기 소자분말 및 시계초분말을 1:1의 중량비율로 혼합하여 제1 혼합분말을 준비하는 단계; 상기 제1 혼합분말 및 물을 혼합하고, 교반을 수행하며 95-105℃의 온도에서 10-14시간 동안 1차 열수추출을 수행하는 단계; 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제1 추출물을 제조하는 단계; 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 남은 고형물을 다시 물과 혼합하고, 135-145℃의 온도에서 4-8시간 동안 2차 열수추출을 수행하는 단계; 2차 열수추출 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제2 추출물을 제조하는 단계; 및 상기 제1 추출물 및 제2 추출물을 혼합하는 단계;를 포함하는 소자 추출물 및 시계초 추출물을 제조하는 단계;Washing the elements and clock grass, cutting them into appropriate sizes, freeze-drying them, and pulverizing to prepare element powder and clock grass powder; preparing a first mixed powder by mixing the element powder and clock start powder in a weight ratio of 1:1; mixing the first mixed powder and water, stirring, and performing primary hot water extraction at a temperature of 95-105° C. for 10-14 hours; After performing the first hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a first extract; After performing the first hot water extraction, filtering with a filter paper, mixing the remaining solids with water again, and performing a second hot water extraction at a temperature of 135-145 ° C. for 4-8 hours; After the second hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a second extract; And the step of mixing the first extract and the second extract; preparing a device extract and passion fruit extract containing;
오미자 및 복숭아를 파쇄한 후 세척하여 오미자씨 및 복숭아씨를 분리하는 단계; 분리된 오미자씨 및 복숭아씨를 분쇄하여 오미자씨분말 및 복숭아씨분말을 준비하는 단계; 상기 오미자씨분말 및 복숭아씨분말을 1:1의 중량비율로 혼합하여 제2 혼합분말을 준비하는 단계; 상기 제2 혼합분말 및 헥산을 혼합하고, 28-32℃의 온도에서 2-4일 동안 교반하여 추출물을 제조하는 단계; 및 상기 추출물을 여과하고 감압농축한 후 원심분리하여 이물질을 제거한 후, 감압증류하는 단계;를 포함하는 오미자씨 오일 및 복숭아씨 오일을 제조하는 단계;Separating Schizandra seeds and peach seeds by washing after crushing Schizandra chinensis and peaches; Preparing Schisandra seed powder and peach seed powder by grinding the separated Schizandra seeds and peach seeds; Preparing a second mixed powder by mixing the Schizandra seed powder and peach seed powder in a weight ratio of 1: 1; Preparing an extract by mixing the second mixed powder and hexane and stirring at a temperature of 28-32 ° C for 2-4 days; Preparing Omija seed oil and peach seed oil comprising; and filtering and concentrating the extract under reduced pressure, centrifuging to remove foreign matter, and then distilling under reduced pressure;
호박씨를 끓인 물로 세척하고, 분쇄하여 호박씨분말을 준비하는 단계; 호박씨 및 물을 혼합하여 혼합물을 제조하고, 혼합물에 효모균으로 사카로미케스 케레비시아이(Saccharomyces cerevisiae)를 첨가한 후 38-42℃의 온도에서 34-38시간 동안 발효시키는 단계; 발효된 혼합물 및 부틸렌글리콜을 혼합하고, 28-32℃의 온도에서 4-6일 동안 교반하여 추출물을 제조하는 단계; 및 상기 추출물을 여과 및 감압농축한 후 동결건조하는 단계;를 포함하는 호박씨 발효추출물을 제조하는 단계;Preparing pumpkin seed powder by washing and grinding pumpkin seeds with boiled water; Mixing pumpkin seeds and water to prepare a mixture, adding Saccharomyces cerevisiae as a yeast to the mixture, and then fermenting at a temperature of 38-42 ° C. for 34-38 hours; Preparing an extract by mixing the fermented mixture and butylene glycol and stirring at a temperature of 28-32 ° C. for 4-6 days; Preparing a fermented pumpkin seed extract comprising; and filtering and concentrating the extract under reduced pressure and then freeze-drying it.
상기 수삼 유래 엑소좀 1-5 중량부, 상기 소자 유래 엑소좀 1-5 중량부, 아세틸헥사펩타이드-8 0.5-4 중량부, 상기 올리고 히알루론산 4-8 중량부, 피토콜라겐 1-5 중량부, 상기 소자 추출물 0.3-2 중량부, 상기 시계초 추출물 0.3-2 중량부, 스쿠알란 0.5-4 중량부, 펜틸렌글라이콜 0.5-4 중량부, 나이아신아마이드 0.1-1 중량부, 세테아릴알코올 0.5-4 중량부, 글리세릴스테아레이트 0.5-4 중량부, 스테아릴알코올 0.1-1 중량부, 프로판다이올 0.5-4 중량부, 카보머 1.5-3.5 중량부, 리모넨 0.5-4 중량부, 리날룰 0.5-4 중량부 및 당근수 23-27 중량부를 혼합하고, 48-52℃의 온도에서 50-70분 동안 교반하여 제1 혼합물을 제조하는 단계;1-5 parts by weight of the fresh ginseng-derived exosome, 1-5 parts by weight of the soma-derived exosome, 0.5-4 parts by weight of acetyl hexapeptide-8, 4-8 parts by weight of the oligo hyaluronic acid, 1-5 parts by weight of phytocollagen 0.3-2 parts by weight of the soybean extract, 0.3-2 parts by weight of the passion fruit extract, 0.5-4 parts by weight of squalane, 0.5-4 parts by weight of pentylene glycol, 0.1-1 part by weight of niacinamide, 0.5 parts by weight of cetearyl alcohol -4 parts by weight, glyceryl stearate 0.5-4 parts by weight, stearyl alcohol 0.1-1 parts by weight, propanediol 0.5-4 parts by weight, carbomer 1.5-3.5 parts by weight, limonene 0.5-4 parts by weight, linalool Preparing a first mixture by mixing 0.5-4 parts by weight and 23-27 parts by weight of carrots and stirring at a temperature of 48-52 ° C. for 50-70 minutes;
세라마이드 0.5-4 중량부, 상기 오미자씨 오일 0.3-2 중량부, 상기 복숭아씨 오일 0.3-2 중량부, 상기 호박씨 발효추출물 0.3-2 중량부, 은행나무잎 추출물 0.3-2 중량부, 아보카도 추출물 0.3-2 중량부, 글리세린 1-5 중량부, 폴리글리세릴-6스테아레이트 0.1-1 중량부, 폴리글리세릴-2스테아레이트 0.1-1 중량부, 부틸렌글라이콜 2-6 중량부, 카보머 1.5-3.5 중량부, 세틸에틸헥사노에이트 0.5-4 중량부, 솔비톨 0.1-1 중량부, 트로메타민 0.1-1 중량부, 폴리글리세릴-6베헤네이트 0.1-1 중량부, 1,2-헥산다이올 0.1-1 중량부 및 당근수 13-17 중량부를 혼합하고, 78-82℃의 온도에서 20-40분 동안 교반하여 제2 혼합물을 제조하는 단계; 및0.5-4 parts by weight of ceramide, 0.3-2 parts by weight of Schisandra seed oil, 0.3-2 parts by weight of peach seed oil, 0.3-2 parts by weight of fermented pumpkin seed extract, 0.3-2 parts by weight of ginkgo leaf extract, 0.3 parts by weight of avocado extract -2 parts by weight, glycerin 1-5 parts by weight, polyglyceryl-6 stearate 0.1-1 parts by weight, polyglyceryl-2 stearate 0.1-1 parts by weight, butylene glycol 2-6 parts by weight, carbomer 1.5-3.5 parts by weight, cetylethylhexanoate 0.5-4 parts by weight, sorbitol 0.1-1 parts by weight, tromethamine 0.1-1 parts by weight, polyglyceryl-6behenate 0.1-1 parts by weight, 1,2- Preparing a second mixture by mixing 0.1-1 part by weight of hexanediol and 13-17 parts by weight of carrot water and stirring at a temperature of 78-82 ° C. for 20-40 minutes; and
상기 제1 혼합물 및 상기 제2 혼합물을 혼합하고, 63-67℃의 온도에서 20-40분 동안 교반하는 단계;를 포함하는 화장료 조성물의 제조방법을 제공한다.It provides a method for producing a cosmetic composition comprising the step of mixing the first mixture and the second mixture and stirring for 20-40 minutes at a temperature of 63-67 ° C.
본 발명에 따른 화장료 조성물은 피부 장벽개선, 보습에 효과적이다.The cosmetic composition according to the present invention is effective for skin barrier improvement and moisturizing.
도 1은 본 발명의 일 측면에서 제공되는 화장료 조성물의 제조방법을 순서도로 나타낸 것이다.1 is a flow chart showing a method for producing a cosmetic composition provided in one aspect of the present invention.
이하에서는 첨부된 도면을 참조하여 다양한 실시예를 보다 상세하게 설명한다. 본 명세서에 기재된 실시예는 다양하게 변형될 수 있다. 특정한 실시예가 도면에서 묘사되고 상세한 설명에서 자세하게 설명될 수 있다. 그러나 첨부된 도면에 개시된 특정한 실시 예는 다양한 실시예를 쉽게 이해하도록 하기 위한 것일 뿐이다. 따라서 첨부된 도면에 개시된 특정 실시예에 의해 기술적 사상이 제한되는 것은 아니며, 발명의 사상 및 기술 범위에 포함되는 모든 균등물 또는 대체물을 포함하는 것으로 이해되어야 한다.Hereinafter, various embodiments will be described in more detail with reference to the accompanying drawings. The embodiments described in this specification may be modified in various ways. Certain embodiments may be depicted in the drawings and described in detail in the detailed description. However, specific embodiments disclosed in the accompanying drawings are only intended to facilitate understanding of various embodiments. Therefore, the technical idea is not limited by the specific embodiments disclosed in the accompanying drawings, and it should be understood to include all equivalents or substitutes included in the spirit and technical scope of the invention.
1차, 2차, 제1, 제2 등과 같이 서수를 포함하는 용어는 다양한 구성요소들을 설명하는데 사용될 수 있지만, 이러한 구성요소들은 상술한 용어에 의해 한정되지는 않는다. 상술한 용어는 하나의 구성요소를 다른 구성요소로부터 구별하는 목적으로만 사용된다.Terms including ordinal numbers such as primary, secondary, first, and second may be used to describe various elements, but these elements are not limited by the above-mentioned terms. The terminology described above is only used for the purpose of distinguishing one component from another.
본 명세서에서, '포함한다' 또는 '가지다' 등의 용어는 명세서상에 기재된 특징, 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것이 존재함을 지정하려는 것이지, 하나 또는 그 이상의 다른 특징들이나 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것들의 존재 또는 부가 가능성을 미리 배제하지 않는 것으로 이해되어야 한다. 어떤 구성요소가 다른 구성요소에 '연결되어' 있다거나 '접속되어' 있다고 언급된 때에는, 그 다른 구성요소에 직접적으로 연결되어 있거나 또는 접속되어 있을 수도 있지만, 중간에 다른 구성요소가 존재할 수도 있다고 이해되어야 할 것이다. 반면에, 어떤 구성요소가 다른 구성요소에 '직접 연결되어' 있다거나 '직접 접속되어' 있다고 언급된 때에는, 중간에 다른 구성요소가 존재하지 않는 것으로 이해되어야 할 것이다.In this specification, terms such as 'comprise' or 'having' are intended to designate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, but one or more other features It should be understood that the presence or addition of numbers, steps, operations, components, parts, or combinations thereof is not precluded. It is understood that when a component is referred to as being 'connected' or 'connected' to another component, it may be directly connected or connected to the other component, but other components may exist in the middle. It should be. On the other hand, when a component is referred to as being 'directly connected' or 'directly connected' to another component, it should be understood that no other component exists in the middle.
본 명세서에서 용어, '엑소좀(exosomes)'은 세포막의 구조와 동일한 이중인지질막으로 이루어진 수십 내지 수백나노미터(바람직하게는 대략 30~200 nm) 크기의 소포체를 의미한다(단, 분리 대상이 되는 세포 종류, 분리방법 및 측정방법에 따라 엑소좀의 입자 크기는 가변될 수 있음)(Vasiliy S. Chernyshev et al., "Size and shape characterization of hydrated and desiccated exosomes", Anal Bioanal Chem, (2015) DOI 10.1007/s00216-015-8535-3). 엑소좀에는 엑소좀 카고(cargo)라고 불리는 단백질, 핵산(mRNA, miRNA 등) 등이 포함되어 있다. 엑소좀 카고에는 광범위한 신호전달 요소들(signaling factors)이 포함되며, 이들 신호전달 요소들은 세포 타입에 특이적이고 분비세포의 환경에 따라 상이하게 조절되는 것으로 알려져 있다. 엑소좀은 세포가 분비하는 세포간 신호전달 매개체로서 이를 통해 전달된 다양한 세포 신호는 표적 세포의 활성화, 성장, 이동, 분화, 탈분화, 사멸(apoptosis), 괴사(necrosis)를 포함한 세포 행동을 조절한다고 알려져 있다.As used herein, the term 'exosomes' refers to endoplasmic reticulum having a size of tens to hundreds of nanometers (preferably approximately 30 to 200 nm) composed of a double-phospholipid membrane identical to the structure of a cell membrane (provided that the separation target is The particle size of exosomes can vary depending on the type of cell used, isolation method, and measurement method) (Vasiliy S. Chernyshev et al., "Size and shape characterization of hydrated and desiccated exosomes", Anal Bioanal Chem, (2015) DOI 10.1007/s00216-015-8535-3). Exosomes contain proteins and nucleic acids (mRNA, miRNA, etc.), which are called exosome cargo. It is known that the exosome cargo contains a wide range of signaling factors, and these signaling factors are cell type-specific and differently regulated depending on the environment of the secreting cell. Exosomes are intercellular signaling mediators secreted by cells, and various cell signals transmitted through them regulate cell behavior including activation, growth, migration, differentiation, dedifferentiation, apoptosis, and necrosis of target cells. It is known.
본 명세서에서 사용되는 용어, '피부 장벽개선'이라 함은 피부 각질층(stratum corneum)의 피부 장벽기능을 보호, 강화 내지는 개선하는 것을 의미하고, 진피나 피하지방조직의 재생이나 기능 개선을 의미하지 않는다. 예를 들어, '피부 장벽개선'은 피부 각질층의 수분 감소 방지, 예를 들어 TEWL(경피수분손실)과 같은 피부 각질층의 수분감소 지표의 개선, 피부수분 함유량(skin hydration)의 증가, 세라마이드, 디히드로세라마이드 및/또는 스핑고 염기의 생성량 증가 등과 같은 피부장벽지표 개선을 통해 진정한 의미의 피부 각질층의 피부장벽 기능의 보호, 강화 및/또는 개선을 가져오는 것을 의미한다.As used herein, the term 'skin barrier improvement' refers to protecting, strengthening or improving the skin barrier function of the stratum corneum, and does not mean regeneration or functional improvement of the dermis or subcutaneous fat tissue. . For example, 'improvement of skin barrier' means prevention of water loss in the stratum corneum, for example, improvement of stratum corneum water loss index such as TEWL (transepidermal water loss), increase in skin hydration, ceramide, It means to bring about protection, reinforcement and/or improvement of the skin barrier function of the stratum corneum in a true sense through improvement of skin barrier indicators such as increased production of hydroceramide and/or sphingo base.
본 명세서에서 사용되는 용어인 '보습'은 피부의 수분 손실(수분 증발) 등을 적절히 조절하여 생체의 항상성을 유지하는 것을 의미한다. '피부 트러블'이란 외부 자극이나 신체적 변화에 의해 피부가 붉게 변하고 가려움증이 나타나며, 심한 경우 작은 돌기나 뾰루지 등이 생기는 현상을 의미한다.As used herein, the term 'moisturizing' refers to maintaining the homeostasis of a living body by appropriately controlling water loss (moisture evaporation) of the skin. 'Skin trouble' refers to a phenomenon in which the skin turns red and itches due to external stimuli or physical changes, and in severe cases, small bumps or pimples appear.
그 밖에도, 본 발명을 설명함에 있어서, 관련된 공지 기능 혹은 구성에 대한 구체적인 설명이 본 발명의 요지를 불필요하게 흐릴 수 있다고 판단되는 경우, 그에 대한 상세한 설명은 축약하거나 생략한다.In addition, in describing the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be abbreviated or omitted.
본 발명은 수삼 유래 엑소좀, 아세틸헥사펩타이드-8, 올리고 히알루론산, 피토콜라겐 및 세라마이드를 포함하는 화장료 조성물을 제공한다.The present invention provides a cosmetic composition containing fresh ginseng-derived exosomes, acetylhexapeptide-8, oligo hyaluronic acid, phyto-collagen and ceramide.
이하, 본 발명에 따른 화장료 조성물에 대하여 상세히 설명한다.Hereinafter, the cosmetic composition according to the present invention will be described in detail.
본 발명에 따른 화장료 조성물은 수삼 유래 엑소좀을 포함한다. 상기 수삼 유래 엑소좀은 비건엑소좀으로 적용한다.The cosmetic composition according to the present invention includes fresh ginseng-derived exosomes. The fresh ginseng-derived exosomes are applied as vegan exosomes.
수삼은 신의 선물이라고 밖에는 표현할 수 없는 신비한 효능을 가진 한국 대표 특산품이다. 상기 수삼 유래 엑소좀은 노화를 촉진시키는 요인인 활성산소를 없애주는 작용 뿐만 아니라, 산화를 억제하는 뛰어난 항산화 작용으로 노화를 방지하고, 피부 면역을 조절하고 노화로 인해 지친 피부에 탄력과 에너지를 공급해준다. 또한, 사포닌 또는 진세노사이드, 항암 물질 등을 많이 함유하고 있고, 당뇨와 노화 방지 효과를 지닌 항산화물질이 다량 함유되어 있다. Fresh ginseng is a representative Korean product with mysterious efficacy that can only be described as a gift from God. The fresh ginseng-derived exosomes not only eliminate active oxygen, which is a factor that accelerates aging, but also prevent aging with an excellent antioxidant action that inhibits oxidation, regulates skin immunity, and supplies elasticity and energy to tired skin due to aging it does In addition, it contains a lot of saponin or ginsenoside, anticancer substances, and the like, and contains a large amount of antioxidants with anti-diabetic and anti-aging effects.
본 발명에 따른 화장료 조성물은 아세틸헥사펩타이드-8를 포함한다. 상기 아세틸헥사펩타이드-8은 비건펩타이드로 적용되며, SNAP-25과 구조적으로 유사한 물질로 SNAP 25와 경쟁적으로 작용하여 스네어 콤플렉스의 구조를 변형시킨다. 그러면 이 콤플렉스는 신경전달물질을 효과적으로 방출하지 못하므로 근육운동이 약해지게 되어 근육운동에 의한 얼굴의 주름생성을 억제해주는 역할을 한다.The cosmetic composition according to the present invention includes acetyl hexapeptide-8. The acetylhexapeptide-8 is applied as a vegan peptide, and as a substance structurally similar to SNAP-25, it acts competitively with SNAP-25 to modify the structure of the snare complex. Then, since this complex cannot effectively release neurotransmitters, muscle movement is weakened, and it plays a role in suppressing wrinkles on the face caused by muscle movement.
본 발명에 따른 화장료 조성물은 올리고 히알루론산을 포함한다. 올리고 히알루론산은 저분자량을 가지는 히알루론산이고, 특히 바람직하게는 중량평균분자량이 1,000-3,000인 히알루론산 63-67 중량부 및 중량평균분자량이 15,000-20,000인 히알루론산 33-37 중량부를 포함한다. 더욱 바람직하게는 1,000-3,000인 히알루론산 64-66 중량부 및 중량평균분자량이 15,000-20,000인 히알루론산 34-36 중량부를 포함한다. 상기와 같이 저분자량을 가지는 히알루론산 중에서도 상대적으로 더 낮은 분자량을 가지는 히알루론산과 상대적으로 더 높은 분자량을 가지는 히알루론산을 혼합하여 적용함으로써 더욱 우수한 보습효과를 나타낸다. 저분자량의 히알루론산은 대한민국등록특허 10-066591를 참조하여 분자량을 조절한 것을 사용할 수 있다.The cosmetic composition according to the present invention contains oligo hyaluronic acid. Oligo hyaluronic acid is hyaluronic acid having a low molecular weight, and particularly preferably contains 63-67 parts by weight of hyaluronic acid having a weight average molecular weight of 1,000-3,000 and 33-37 parts by weight of hyaluronic acid having a weight average molecular weight of 15,000-20,000. More preferably, it contains 64-66 parts by weight of hyaluronic acid of 1,000-3,000 and 34-36 parts by weight of hyaluronic acid of 15,000-20,000 weight average molecular weight. As described above, among hyaluronic acids having a low molecular weight, hyaluronic acid having a relatively lower molecular weight and hyaluronic acid having a relatively higher molecular weight are mixed and applied to show a more excellent moisturizing effect. Low molecular weight hyaluronic acid can be used by adjusting the molecular weight with reference to Korean Patent Registration 10-066591.
본 발명에 따른 화장료 조성물은 피토콜라겐을 포함한다. 상기 피토콜라겐은 비건콜라겐으로서, 옥수수, 콩, 밀이 만들어내는 단백질 섬유를 엮어 제조되는 식물 유래 콜라겐이다. 상기 피토콜라겐은 수분 보유 능력이 우수하여 보습에 효과적이다.The cosmetic composition according to the present invention includes phyto-collagen. The phytocollagen is a vegan collagen, which is a plant-derived collagen prepared by weaving protein fibers produced by corn, beans, and wheat. The phytocollagen is effective in moisturizing because of its excellent ability to retain moisture.
본 발명에 따른 화장료 조성물은 세라마이드를 포함한다. 피부 장벽 속 가장 풍부한 것은 세라마이드엔피로, 세라마이드를 적용하여 피부 장벽개선에 효과적이다.The cosmetic composition according to the present invention contains ceramide. The most abundant thing in the skin barrier is ceramide NP, which is effective in improving the skin barrier by applying ceramide.
또한, 상기 화장료 조성물은 수삼 유래 엑소좀 1-5 중량부, 소자 유래 엑소좀 1-5 중량부, 아세틸헥사펩타이드-8 0.5-4 중량부, 올리고 히알루론산 4-8 중량부, 피토콜라겐 1-5 중량부, 세라마이드 0.5-4 중량부, 소자 추출물 0.3-2 중량부, 시계초 추출물 0.3-2 중량부, 오미자씨 오일 0.3-2 중량부, 복숭아씨 오일 0.3-2 중량부, 호박씨 발효추출물 0.3-2 중량부, 은행나무잎 추출물 0.3-2 중량부, 아보카도 추출물 0.3-2 중량부, 글리세린 1-5 중량부, 스쿠알란 0.5-4 중량부, 펜틸렌글라이콜 0.5-4 중량부, 나이아신아마이드 0.1-1 중량부, 폴리글리세릴-6스테아레이트 0.1-1 중량부, 폴리글리세릴-2스테아레이트 0.1-1 중량부, 부틸렌글라이콜 2-6 중량부, 세테아릴알코올 0.5-4 중량부, 글리세릴스테아레이트 0.5-4 중량부, 스테아릴알코올 0.1-1 중량부, 프로판다이올 0.5-4 중량부, 카보머 3-7 중량부, 세틸에틸헥사노에이트 0.5-4 중량부, 솔비톨 0.1-1 중량부, 트로메타민 0.1-1 중량부, 폴리글리세릴-6베헤네이트 0.1-1 중량부, 1,2-헥산다이올 0.1-1 중량부, 리모넨 0.5-4 중량부, 리날룰 0.5-4 중량부 및 당근수 38-42 중량부를 포함하는 것이 바람직하고, 수삼 유래 엑소좀 2-4 중량부, 소자 유래 엑소좀 2-4 중량부, 아세틸헥사펩타이드-8 1-3 중량부, 올리고 히알루론산 5-7 중량부, 피토콜라겐 2-4 중량부, 세라마이드 1-3 중량부, 소자 추출물 0.5-1.5 중량부, 시계초 추출물 0.5-1.5 중량부, 오미자씨 오일 0.5-1.5 중량부, 복숭아씨 오일 0.5-1.5 중량부, 호박씨 발효추출물 0.5-1.5 중량부, 은행나무잎 추출물 0.5-1.5 중량부, 아보카도 추출물 0.5-1.5 중량부, 글리세린 2-4 중량부, 스쿠알란 1-3 중량부, 펜틸렌글라이콜 1-3 중량부, 나이아신아마이드 0.3-0.7 중량부, 폴리글리세릴-6스테아레이트 0.3-0.7 중량부, 폴리글리세릴-2스테아레이트 0.3-0.7 중량부, 부틸렌글라이콜 3-5 중량부, 세테아릴알코올 1-3 중량부, 글리세릴스테아레이트 1-3 중량부, 스테아릴알코올 0.3-0.7 중량부, 프로판다이올 1-3 중량부, 카보머 4-6 중량부, 세틸에틸헥사노에이트 1-3 중량부, 솔비톨 0.3-0.7 중량부, 트로메타민 0.3-0.7 중량부, 폴리글리세릴-6베헤네이트 0.3-0.7 중량부, 1,2-헥산다이올 0.3-0.7 중량부, 리모넨 1-3 중량부, 리날룰 1-3 중량부 및 당근수 39-41 중량부를 포함하는 것이 더욱 바람직하다.In addition, the cosmetic composition contains 1-5 parts by weight of fresh ginseng-derived exosomes, 1-5 parts by weight of soybean-derived exosomes, 0.5-4 parts by weight of acetyl hexapeptide-8, 4-8 parts by weight of oligo hyaluronic acid, phytocollagen 1- 5 parts by weight, ceramide 0.5-4 parts by weight, soja extract 0.3-2 parts by weight, passion fruit extract 0.3-2 parts by weight, Schizandra seed oil 0.3-2 parts by weight, peach seed oil 0.3-2 parts by weight, pumpkin seed fermented extract 0.3 parts by weight -2 parts by weight, ginkgo leaf extract 0.3-2 parts by weight, avocado extract 0.3-2 parts by weight, glycerin 1-5 parts by weight, squalane 0.5-4 parts by weight, pentylene glycol 0.5-4 parts by weight, niacinamide 0.1 -1 part by weight, 0.1-1 part by weight of polyglyceryl-6 stearate, 0.1-1 part by weight of polyglyceryl-2 stearate, 2-6 parts by weight of butylene glycol, 0.5-4 part by weight of cetearyl alcohol 0.5-4 parts by weight of glyceryl stearate, 0.1-1 part by weight of stearyl alcohol, 0.5-4 parts by weight of propanediol, 3-7 parts by weight of carbomer, 0.5-4 parts by weight of cetylethylhexanoate, 0.1-4 parts by weight of sorbitol -1 part by weight, 0.1-1 part by weight of tromethamine, 0.1-1 part by weight of polyglyceryl-6behenate, 0.1-1 part by weight of 1,2-hexanediol, 0.5-4 part by weight of limonene, 0.5 part by weight of linalool -4 parts by weight and preferably containing 38-42 parts by weight of carrots, 2-4 parts by weight of fresh ginseng-derived exosomes, 2-4 parts by weight of soybean-derived exosomes, 1-3 parts by weight of acetyl hexapeptide-8, oligo 5-7 parts by weight of hyaluronic acid, 2-4 parts by weight of phytocollagen, 1-3 parts by weight of ceramide, 0.5-1.5 parts by weight of Soja extract, 0.5-1.5 parts by weight of passion fruit extract, 0.5-1.5 parts by weight of Schisandra seed oil, peach Seed oil 0.5-1.5 parts by weight, pumpkin seed fermented extract 0.5-1.5 parts by weight, ginkgo leaf extract 0.5-1.5 parts by weight, avocado extract 0.5-1.5 parts by weight, glycerin 2-4 parts by weight, squalane 1-3 parts by weight, pentyl Len glycol 1-3 parts by weight, niacinamide 0.3-0.7 parts by weight, polyglyceryl-6 stearate 0.3- 0.7 parts by weight, polyglyceryl-2 stearate 0.3-0.7 parts by weight, butylene glycol 3-5 parts by weight, cetearyl alcohol 1-3 parts by weight, glyceryl stearate 1-3 parts by weight, stearyl alcohol 0.3-0.7 parts by weight, 1-3 parts by weight of propanediol, 4-6 parts by weight of carbomer, 1-3 parts by weight of cetylethylhexanoate, 0.3-0.7 parts by weight of sorbitol, 0.3-0.7 parts by weight of tromethamine, 0.3-0.7 parts by weight of polyglyceryl-6behenate, 0.3-0.7 parts by weight of 1,2-hexanediol, 1-3 parts by weight of limonene, 1-3 parts by weight of linalool and 39-41 parts by weight of carrots it is more preferable
소자(Perillae Semen)는 꿀풀과(Labiatae)에 속하는 일년생 초본인 차조기(Perilla frutescens var. acuta)의 과실로서 거담, 변비치료 등의 목적으로 사용된다. 소자(Perillae Semem)는 차조기(소엽)의 씨를 말하는 것으로 발한, 진해, 건위, 이뇨, 진정 및 진통제로 사용한다. Perillae Semen is the fruit of Perilla frutescens var. Soja (Perillae Semem) refers to the seeds of perilla (lobule) and is used as a sweating, antitussive, stomach, diuretic, sedative and analgesic.
상기 올리고 히알루론산은 중량평균분자량이 1,000-3,000인 히알루론산 63-67 중량부 및 중량평균분자량이 15,000-20,000인 히알루론산 33-37 중량부를 포함한다. 더욱 바람직하게는 1,000-3,000인 히알루론산 64-66 중량부 및 중량평균분자량이 15,000-20,000인 히알루론산 34-36 중량부를 포함한다. 상기와 같이 저분자량을 가지는 히알루론산 중에서도 상대적으로 더 낮은 분자량을 가지는 히알루론산과 상대적으로 더 높은 분자량을 가지는 히알루론산을 혼합하여 적용함으로써 더욱 우수한 보습효과를 나타낸다.The oligo hyaluronic acid includes 63-67 parts by weight of hyaluronic acid having a weight average molecular weight of 1,000-3,000 and 33-37 parts by weight of hyaluronic acid having a weight average molecular weight of 15,000-20,000. More preferably, it contains 64-66 parts by weight of hyaluronic acid of 1,000-3,000 and 34-36 parts by weight of hyaluronic acid of 15,000-20,000 weight average molecular weight. As described above, among hyaluronic acids having a low molecular weight, hyaluronic acid having a relatively lower molecular weight and hyaluronic acid having a relatively higher molecular weight are mixed and applied to show a more excellent moisturizing effect.
소자 추출물을 포함하여 건조로 가려운 피부 개선을 위한 진정성 고보습 케어가 가능하다.Including soja extract, it is possible to provide soothing, highly moisturizing care to improve itchy skin due to dryness.
오미자(Schizandra chinensis Baillon, Omija)는 동의보감 (東醫寶鑑)에 의하면 시고, 달고, 쓰고, 맵고, 짠 다섯 가지의 맛을 가지고 있는 생약으로 예로부터 거담, 진해, 정천, 철혈, 검한, 생전지갈 등 여러가지의 약효가 있고 anthocyanin 뿐만 아니라 flavonoid 및 유기산(有機酸)류 등이 풍부하여 한방에서는 거담자 양강장제(祛痰滋養强壯劑)등으로 이용 되었으며, 또한 간장보호, 알코올해독, 혈당강하, 콜레스테롤저하, 고지혈증완화, 면역조절, 항암 및 항종양 등 다양한 생리적 활성을 나타낸다(Yoon J.E, Kim N.K, Hwang CY, Jo EH, Park MC. Experimental study about the effect of several herbs on collagen synthesis. J Korean Orient Med Ophthalmol Otolaryngol Dermato. 2010 ; 23(3) : 33-41). 또한 리그난은 85% 이상이 오미자씨에 들어 있어 씨에 항암, 항산화 효과를 나타내는 성분이 과량 포함되어 있다. 나아가 오미자 씨에 든 지방은 73%가 '리놀레산'으로 나타났다. 리놀레산은 몸에 좋은 식물성 오메가3지방산으로 심혈관 질환 예방에 도움이 된다. According to Donguibogam (東醫寶鑑), Schizandra chinensis Baillon (Omija) is a herbal medicine that has five tastes: sour, sweet, bitter, spicy, and salty. It has various medicinal effects such as anthocyanin, as well as flavonoids and organic acids, so it was used as an expectorant and galactant in oriental medicine, and also protects the liver, detoxifies alcohol, lowers blood sugar, cholesterol It exhibits various physiological activities such as reduction of hyperlipidemia, immunomodulation, anticancer and antitumor (Yoon J.E, Kim N.K, Hwang CY, Jo EH, Park MC. Experimental study about the effect of several herbs on collagen synthesis. J Korean Orient Med Ophthalmol Otolaryngol Dermato. 2010;23(3):33-41). In addition, more than 85% of lignans are contained in Schisandra seeds, so the seeds contain an excessive amount of ingredients that exhibit anti-cancer and antioxidant effects. Furthermore, 73% of the fat in Schisandra seeds was 'linoleic acid'. Linoleic acid is a vegetable omega-3 fatty acid that is good for the body and helps prevent cardiovascular disease.
복숭아나무(Prunus persica (L.) Batsch)는 중국 황허강[黃河]유역의 고원지대에 그 근원을 두고 있으며, 현재는 한국을 포함하여 미국, 이탈리아, 에스파냐 등지에서 재배되는 식물로 중국, 한국의 전통의학에서 나무껍질, 나뭇잎, 꽃, 열매, 복숭아씨, 복숭아 털, 복숭아벌레까지 모두 약제로 쓰이는 것으로 알려져 있다. 그 중 복숭아씨(Semen Persicae)는 주성분인 글리세리드(glyceride), 스테롤(sterol), 에물신(emulsin), 아미그달린(amygdalin)으로 이루어져 있고, 말초혈액 순환불량 증후군 (Oketsu)을 차단하는 효과가 있는 것으로 보고되었으며(Kosuge T., Chem. Pharm. Bull., 33, pp.1496-1498, 1985), 강력한 항종양 촉진자(anti-tumor promoter)와 긴밀하게 연관되어 있고, 부인병을 치료하는 데에도 사용되는 것으로 알려져 있다 (Fukuda, T., Biol. Pharm. Bull., 26, pp.271-273, 2003).The peach tree (Prunus persica (L.) Batsch) has its origins in the highlands of the Yellow River basin in China, and is now cultivated in Korea, the United States, Italy, and Spain. In traditional medicine, bark, leaves, flowers, fruits, peach seeds, peach hairs, and peach bugs are all known to be used as medicines. Among them, peach seed (Semen Persicae) is composed of glyceride, sterol, emulsin, and amygdalin as main components, and is effective in blocking peripheral blood circulation syndrome (Oketsu). has been reported (Kosuge T., Chem. Pharm. Bull., 33, pp.1496-1498, 1985), is closely related to a potent anti-tumor promoter, and is also used to treat gynecological diseases. (Fukuda, T., Biol. Pharm. Bull., 26, pp.271-273, 2003).
호박씨(Cucurbita Pepo (Pumpkin) Seed)” 는 호박에 2∼3% 들어 있는 유량종실로서 베타-카로틴(β-carotene), 식물스테롤(phytosterol)의 함량이 높고 약40% 정도의 지방과 30% 정도의 단백질을 함유한다. 민간에서는 전립선 비대증의 치료, 이뇨, 구충에 유효한 성분이 들어있는 것으로 구전되어 민간 한약재로 이용되어 왔다.“Cucurbita Pepo (Pumpkin) Seed” is a milk seed that contains 2-3% of pumpkin and has high content of β-carotene and phytosterol, about 40% fat and about 30% contains the protein of In the private sector, it has been used as a folk herbal medicine because it is said to contain ingredients effective for the treatment of benign prostatic hyperplasia, diuresis, and anthelmintic.
은행나무 잎(Ginkgo biloba leaf)은 동아시아에서 오랜 시간 동안 약재로 사용되어 왔다. 현재에는 전세계적으로 천연 건강기능식품 또는 보충제로서 널리 이용되고 있으며, 특히, 뇌 순환 장애, 알츠하이머 병, 단기 기억 상실, 우울증 등을 포함한 다양한 질병의 개선에 효과적인 것으로 알려져 있다. 상기 은행나무잎 추출물은 플라보노이드 성분이 풍부하여 유사 보톡스 효과를 기대할 수 있다. Ginkgo biloba leaf has been used medicinally in East Asia for a long time. Currently, it is widely used as a natural health functional food or supplement worldwide, and is known to be effective in improving various diseases, including cerebral circulation disorder, Alzheimer's disease, short-term memory loss, and depression. The ginkgo biloba leaf extract is rich in flavonoids, so a similar botox effect can be expected.
아보카도는 과일 중 비타민과 미네랄이 가장 풍부하기 때문에 숲속의 버터라고도 불리며, 아보카도 추출물은 UV를 비롯한 다양한 외부환경으로부터 산화된 단백질을 제거함으로써 피부 내에서 히알루론산 합성을 촉진시켜 우수한 보습효과를 부여함과 동시에 재생 효과를 나타낸다.Avocado is also called forest butter because it is rich in vitamins and minerals among fruits, and avocado extract removes oxidized proteins from various external environments including UV to promote hyaluronic acid synthesis in the skin to provide excellent moisturizing effect. At the same time, it shows a regenerative effect.
당근은 90%의 물을 함유한 수분 함량이 높은 채소로, 비타민 A, K와 미네랄을 함유하여 거칠어진 피부를 빠르게 회복시켜주는 영양성분이 풍부하다. 또한, 당근의 수분은 피부의 보습에 좋은 영향을 준다. 상기 당근수는 수용성 원료로 당근이 가진 풍부한 수분과 미네랄을 녹여낸 원료로서 일반적으로 화장료 조성물에 적용되는 정제수를 대신하여 사용한다.Carrots are vegetables with high water content, containing 90% of water, and are rich in nutrients that quickly restore rough skin by containing vitamins A, K and minerals. In addition, the moisture of carrots has a good effect on moisturizing the skin. The carrot water is a water-soluble raw material in which the rich moisture and minerals of carrots are dissolved, and is generally used instead of purified water applied to cosmetic compositions.
또한, 본 발명은In addition, the present invention
수삼을 세척하여 18-22℃의 온도에서 140-160 MPa의 압력으로 3-7분 동안 전처리하는 단계; 전처리된 수삼을 착즙하여 수삼 착즙액을 제조하는 단계; 상기 수삼 착즙액을 원심분리하여 엑소좀을 포함하는 상층액을 수득하는 단계; 상기 엑소좀을 포함하는 상층액을 동결건조하여 동결건조물을 제조하는 단계; 상기 동결건조물에 폴리에틸렌글리콜/덱스트란을 사용하여 수성 2상계를 형성하는 단계; 및 상기 수성 2상계 내 엑소좀이 농축된 하층액을 수득하는 단계;를 포함하는 수삼 유래 엑소좀을 제조하는 단계;Washing fresh ginseng and pre-treating it at a temperature of 18-22°C and a pressure of 140-160 MPa for 3-7 minutes; Preparing fresh ginseng juice by extracting pretreated fresh ginseng; Obtaining a supernatant containing exosomes by centrifuging the fresh ginseng juice; preparing a lyophilisate by freeze-drying the supernatant containing the exosomes; forming an aqueous two-phase system using polyethylene glycol/dextran in the lyophilisate; and obtaining a lower layer in which exosomes are concentrated in the aqueous two-phase system; preparing fresh ginseng-derived exosomes;
소자를 세척하여 28-32℃의 온도에서 290-310 MPa의 압력으로 1-5분 동안 전처리하는 단계; 전처리된 소자를 착즙하여 소자 착즙액을 제조하는 단계; 상기 소자 착즙액을 원심분리하여 엑소좀을 포함하는 상층액을 수득하는 단계; 상기 엑소좀을 포함하는 상층액을 동결건조하여 동결건조물을 제조하는 단계; 상기 동결건조물에 폴리에틸렌글리콜/덱스트란을 사용하여 수성 2상계를 형성하는 단계; 및 상기 수성 2상계 내 엑소좀이 농축된 하층액을 수득하는 단계;를 포함하는 소자 유래 엑소좀을 제조하는 단계;washing the device and pre-treating it at a temperature of 28-32° C. and a pressure of 290-310 MPa for 1-5 minutes; Preparing a device juice by extracting the pretreated device; Obtaining a supernatant containing exosomes by centrifuging the juice from the cow; preparing a lyophilisate by freeze-drying the supernatant containing the exosomes; forming an aqueous two-phase system using polyethylene glycol/dextran in the lyophilisate; and obtaining an exosome-enriched lower layer in the aqueous two-phase system; preparing a device-derived exosome comprising;
중량평균분자량이 1,000-3,000인 히알루론산 63-67 중량부 및 중량평균분자량이 15,000-20,000인 히알루론산 33-37 중량부를 혼합하여 혼합물을 제조하는 단계; 및 상기 혼합물을 33-37℃의 온도에서 2-6시간 동안 교반하는 단계;를 포함하는 올리고 히알루론산을 제조하는 단계;Preparing a mixture by mixing 63-67 parts by weight of hyaluronic acid having a weight average molecular weight of 1,000-3,000 and 33-37 parts by weight of hyaluronic acid having a weight average molecular weight of 15,000-20,000; and stirring the mixture at a temperature of 33-37° C. for 2-6 hours; preparing an oligo hyaluronic acid comprising;
소자 및 시계초를 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 소자분말 및 시계초분말을 준비하는 단계; 상기 소자분말 및 시계초분말을 1:1의 중량비율로 혼합하여 제1 혼합분말을 준비하는 단계; 상기 제1 혼합분말 및 물을 혼합하고, 교반을 수행하며 95-105℃의 온도에서 10-14시간 동안 1차 열수추출을 수행하는 단계; 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제1 추출물을 제조하는 단계; 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 남은 고형물을 다시 물과 혼합하고, 135-145℃의 온도에서 4-8시간 동안 2차 열수추출을 수행하는 단계; 2차 열수추출 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제2 추출물을 제조하는 단계; 및 상기 제1 추출물 및 제2 추출물을 혼합하는 단계;를 포함하는 소자 추출물 및 시계초 추출물을 제조하는 단계;Washing the elements and clock grass, cutting them into appropriate sizes, freeze-drying them, and pulverizing to prepare element powder and clock grass powder; preparing a first mixed powder by mixing the element powder and clock start powder in a weight ratio of 1:1; mixing the first mixed powder and water, stirring, and performing primary hot water extraction at a temperature of 95-105° C. for 10-14 hours; After performing the first hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a first extract; After performing the first hot water extraction, filtering with a filter paper, mixing the remaining solids with water again, and performing a second hot water extraction at a temperature of 135-145 ° C. for 4-8 hours; After the second hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a second extract; And the step of mixing the first extract and the second extract; preparing a device extract and passion fruit extract containing;
오미자 및 복숭아를 파쇄한 후 세척하여 오미자씨 및 복숭아씨를 분리하는 단계; 분리된 오미자씨 및 복숭아씨를 분쇄하여 오미자씨분말 및 복숭아씨분말을 준비하는 단계; 상기 오미자씨분말 및 복숭아씨분말을 1:1의 중량비율로 혼합하여 제2 혼합분말을 준비하는 단계; 상기 제2 혼합분말 및 헥산을 혼합하고, 28-32℃의 온도에서 2-4일 동안 교반하여 추출물을 제조하는 단계; 및 상기 추출물을 여과하고 감압농축한 후 원심분리하여 이물질을 제거한 후, 감압증류하는 단계;를 포함하는 오미자씨 오일 및 복숭아씨 오일을 제조하는 단계;Separating Schizandra seeds and peach seeds by washing after crushing Schizandra chinensis and peaches; Preparing Schisandra seed powder and peach seed powder by grinding the separated Schizandra seeds and peach seeds; Preparing a second mixed powder by mixing the Schizandra seed powder and peach seed powder in a weight ratio of 1: 1; Preparing an extract by mixing the second mixed powder and hexane and stirring at a temperature of 28-32 ° C for 2-4 days; Preparing Omija seed oil and peach seed oil comprising; and filtering and concentrating the extract under reduced pressure, centrifuging to remove foreign matter, and then distilling under reduced pressure;
호박씨를 끓인 물로 세척하고, 분쇄하여 호박씨분말을 준비하는 단계; 호박씨 및 물을 혼합하여 혼합물을 제조하고, 혼합물에 효모균으로 사카로미케스 케레비시아이(Saccharomyces cerevisiae)를 첨가한 후 38-42℃의 온도에서 34-38시간 동안 발효시키는 단계; 발효된 혼합물 및 부틸렌글리콜을 혼합하고, 28-32℃의 온도에서 4-6일 동안 교반하여 추출물을 제조하는 단계; 및 상기 추출물을 여과 및 감압농축한 후 동결건조하는 단계;를 포함하는 호박씨 발효추출물을 제조하는 단계;Preparing pumpkin seed powder by washing and grinding pumpkin seeds with boiled water; Mixing pumpkin seeds and water to prepare a mixture, adding Saccharomyces cerevisiae as a yeast to the mixture, and then fermenting at a temperature of 38-42 ° C. for 34-38 hours; Preparing an extract by mixing the fermented mixture and butylene glycol and stirring at a temperature of 28-32 ° C. for 4-6 days; Preparing a fermented pumpkin seed extract comprising; and filtering and concentrating the extract under reduced pressure and then freeze-drying it.
상기 수삼 유래 엑소좀 1-5 중량부, 상기 소자 유래 엑소좀 1-5 중량부, 아세틸헥사펩타이드-8 0.5-4 중량부, 상기 올리고 히알루론산 4-8 중량부, 피토콜라겐 1-5 중량부, 상기 소자 추출물 0.3-2 중량부, 상기 시계초 추출물 0.3-2 중량부, 스쿠알란 0.5-4 중량부, 펜틸렌글라이콜 0.5-4 중량부, 나이아신아마이드 0.1-1 중량부, 세테아릴알코올 0.5-4 중량부, 글리세릴스테아레이트 0.5-4 중량부, 스테아릴알코올 0.1-1 중량부, 프로판다이올 0.5-4 중량부, 카보머 1.5-3.5 중량부, 리모넨 0.5-4 중량부, 리날룰 0.5-4 중량부 및 당근수 23-27 중량부를 혼합하고, 48-52℃의 온도에서 50-70분 동안 교반하여 제1 혼합물을 제조하는 단계;1-5 parts by weight of the fresh ginseng-derived exosome, 1-5 parts by weight of the soma-derived exosome, 0.5-4 parts by weight of acetyl hexapeptide-8, 4-8 parts by weight of the oligo hyaluronic acid, 1-5 parts by weight of phytocollagen 0.3-2 parts by weight of the soybean extract, 0.3-2 parts by weight of the passion fruit extract, 0.5-4 parts by weight of squalane, 0.5-4 parts by weight of pentylene glycol, 0.1-1 part by weight of niacinamide, 0.5 parts by weight of cetearyl alcohol -4 parts by weight, glyceryl stearate 0.5-4 parts by weight, stearyl alcohol 0.1-1 parts by weight, propanediol 0.5-4 parts by weight, carbomer 1.5-3.5 parts by weight, limonene 0.5-4 parts by weight, linalool Preparing a first mixture by mixing 0.5-4 parts by weight and 23-27 parts by weight of carrots and stirring at a temperature of 48-52 ° C. for 50-70 minutes;
세라마이드 0.5-4 중량부, 상기 오미자씨 오일 0.3-2 중량부, 상기 복숭아씨 오일 0.3-2 중량부, 상기 호박씨 발효추출물 0.3-2 중량부, 은행나무잎 추출물 0.3-2 중량부, 아보카도 추출물 0.3-2 중량부, 글리세린 1-5 중량부, 폴리글리세릴-6스테아레이트 0.1-1 중량부, 폴리글리세릴-2스테아레이트 0.1-1 중량부, 부틸렌글라이콜 2-6 중량부, 카보머 1.5-3.5 중량부, 세틸에틸헥사노에이트 0.5-4 중량부, 솔비톨 0.1-1 중량부, 트로메타민 0.1-1 중량부, 폴리글리세릴-6베헤네이트 0.1-1 중량부, 1,2-헥산다이올 0.1-1 중량부 및 당근수 13-17 중량부를 혼합하고, 78-82℃의 온도에서 20-40분 동안 교반하여 제2 혼합물을 제조하는 단계; 및0.5-4 parts by weight of ceramide, 0.3-2 parts by weight of Schisandra seed oil, 0.3-2 parts by weight of peach seed oil, 0.3-2 parts by weight of fermented pumpkin seed extract, 0.3-2 parts by weight of ginkgo leaf extract, 0.3 parts by weight of avocado extract -2 parts by weight, glycerin 1-5 parts by weight, polyglyceryl-6 stearate 0.1-1 parts by weight, polyglyceryl-2 stearate 0.1-1 parts by weight, butylene glycol 2-6 parts by weight, carbomer 1.5-3.5 parts by weight, cetylethylhexanoate 0.5-4 parts by weight, sorbitol 0.1-1 parts by weight, tromethamine 0.1-1 parts by weight, polyglyceryl-6behenate 0.1-1 parts by weight, 1,2- Preparing a second mixture by mixing 0.1-1 part by weight of hexanediol and 13-17 parts by weight of carrot water and stirring at a temperature of 78-82 ° C. for 20-40 minutes; and
상기 제1 혼합물 및 상기 제2 혼합물을 혼합하고, 63-67℃의 온도에서 20-40분 동안 교반하는 단계;를 포함하는 화장료 조성물의 제조방법을 제공한다.It provides a method for producing a cosmetic composition comprising the step of mixing the first mixture and the second mixture and stirring for 20-40 minutes at a temperature of 63-67 ° C.
이때, 도 1에 본 발명에 따른 화장료 조성물의 제조방법을 순서도로 나타내었으며, 이하, 도 1을 참조하여 본 발명에 따른 화장료 조성물의 제조방법을 각 단계별로 상세히 설명한다.At this time, the manufacturing method of the cosmetic composition according to the present invention in Figure 1 is shown in a flow chart, hereinafter, with reference to Figure 1, the manufacturing method of the cosmetic composition according to the present invention will be described in detail for each step.
먼저, 본 발명에 따른 화장료 조성물의 제조방법은 수삼 유래 엑소좀을 제조하는 단계를 포함한다. 상기 수삼 유래 엑소좀을 제조하는 단계는, 수삼을 세척하여 18-22℃의 온도에서 140-160 MPa의 압력으로 3-7분 동안 전처리하는 단계; 전처리된 수삼을 착즙하여 수삼 착즙액을 제조하는 단계; 상기 수삼 착즙액을 원심분리하여 엑소좀을 포함하는 상층액을 수득하는 단계; 상기 엑소좀을 포함하는 상층액을 동결건조하여 동결건조물을 제조하는 단계; 상기 동결건조물에 폴리에틸렌글리콜/덱스트란을 사용하여 수성 2상계를 형성하는 단계; 및 상기 수성 2상계 내 엑소좀이 농축된 하층액을 수득하는 단계;를 포함한다.First, the manufacturing method of the cosmetic composition according to the present invention includes preparing fresh ginseng-derived exosomes. The step of preparing the fresh ginseng-derived exosomes may include washing fresh ginseng and pre-treating the fresh ginseng at a temperature of 18-22° C. and a pressure of 140-160 MPa for 3-7 minutes; Preparing fresh ginseng juice by extracting pretreated fresh ginseng; Obtaining a supernatant containing exosomes by centrifuging the fresh ginseng juice; preparing a lyophilisate by freeze-drying the supernatant containing the exosomes; forming an aqueous two-phase system using polyethylene glycol/dextran in the lyophilisate; and obtaining a lower layer in which exosomes are concentrated in the aqueous two-phase system.
수삼을 세척한 후 초고압기를 이용하여 상온에서 140-160 MPa의 압력으로 3-7분 동안 전처리한다. After washing the fresh ginseng, it is pretreated for 3-7 minutes at a pressure of 140-160 MPa at room temperature using an ultra-high pressure machine.
다음, 수삼을 착즙하여 착즙액을 제조한다. 착즙은 스크류를 가진 착즙기를 이용하여 수행한다.Next, fresh ginseng is squeezed to prepare a juice solution. Juicing is performed using a juicer with a screw.
다음, 수삼 착즙액을 원심분리하여 상층액을 수득한다. 상기 상층액은 엑소좀을 포함한다. 상기 원심분리는 10,000 xg에서 수행한다.Next, the fresh ginseng juice is centrifuged to obtain a supernatant. The supernatant contains exosomes. The centrifugation is performed at 10,000 xg.
다음, 상층액을 동결건조하여 동결건조물을 제조한다. 동결건조기를 이용하여 수행할 수 있다.Next, the supernatant is freeze-dried to prepare a lyophilisate. It can be carried out using a lyophilizer.
다음, 동결건조물에 폴리에틸렌글리콜염, 특히 폴리에틸렌글리콜 및 염으로는 덱스트란(dextran)을 사용하여 수성 2상계를 형성한다. 엑소좀을 효율적으로 분리 정제하기 위해 수성 2상분배법을 사용한다.Next, an aqueous two-phase system is formed by using a polyethylene glycol salt, particularly polyethylene glycol and dextran as the salt, in the lyophilized product. An aqueous two-phase partitioning method is used to efficiently separate and purify exosomes.
상기 폴리에틸렌글리콜은 중량평균분자량이 10,000-35,000인 것을 1-15 중량%, 바람직하게는 2-5 중량% 사용하고 덱스트란은 중량평균분자량이 300,000-650,000인 것을 1-8 중량%, 바람직하게는 1-3 중량% 사용하는 것을 특징으로 한다. 상기 폴리에틸렌글리콜과 덱스트란이 3.3 중량% : 1.7 중량%의 농도비율로 사용되는 경우에 엑소좀의 수율이 가장 높고, 안정성이 가장 우수하므로 더욱 바람직하다.The polyethylene glycol has a weight average molecular weight of 10,000-35,000 and is used at 1-15% by weight, preferably 2-5% by weight, and dextran has a weight average molecular weight of 300,000-650,000 at 1-8% by weight, preferably It is characterized by using 1-3% by weight. When the polyethylene glycol and dextran are used in a concentration ratio of 3.3% by weight: 1.7% by weight, the yield of exosomes is the highest and the stability is the best, so it is more preferable.
다음, 수성 2상계 내 엑소좀이 농축된 하층액을 수득한다.Next, a lower layer in which the exosomes in the aqueous two-phase system are concentrated is obtained.
다음으로, 본 발명에 따른 화장료 조성물의 제조방법은 소자 유래 엑소좀을 제조하는 단계를 포함한다. 상기 소자 유래 엑소좀을 제조하는 단계는, 소자를 세척하여 28-32℃의 온도에서 290-310 MPa의 압력으로 1-5분 동안 전처리하는 단계; 전처리된 소자를 착즙하여 소자 착즙액을 제조하는 단계; 상기 소자 착즙액을 원심분리하여 엑소좀을 포함하는 상층액을 수득하는 단계; 상기 엑소좀을 포함하는 상층액을 동결건조하여 동결건조물을 제조하는 단계; 상기 동결건조물에 폴리에틸렌글리콜/덱스트란을 사용하여 수성 2상계를 형성하는 단계; 및 상기 수성 2상계 내 엑소좀이 농축된 하층액을 수득하는 단계;를 포함한다.Next, the manufacturing method of the cosmetic composition according to the present invention includes preparing a device-derived exosome. The step of preparing the device-derived exosome may include washing the device and pre-treating the device at a temperature of 28-32° C. and a pressure of 290-310 MPa for 1-5 minutes; Preparing a device juice by extracting the pretreated device; Obtaining a supernatant containing exosomes by centrifuging the juice from the cow; preparing a lyophilisate by freeze-drying the supernatant containing the exosomes; forming an aqueous two-phase system using polyethylene glycol/dextran in the lyophilisate; and obtaining a lower layer in which exosomes are concentrated in the aqueous two-phase system.
소자를 세척한 후 초고압기를 이용하여 상온보다 다소 높은 온도에서 290-310 MPa의 압력으로 1-5분 동안 전처리한다. After cleaning the device, it is pretreated for 1-5 minutes at a pressure of 290-310 MPa at a temperature slightly higher than room temperature using an ultra-high pressure machine.
다음, 소자를 착즙하여 착즙액을 제조한다. 착즙은 스크류를 가진 착즙기를 이용하여 수행한다.Next, the device is juiced to prepare a juice solution. Juicing is performed using a juicer with a screw.
다음, 소자 착즙액을 원심분리하여 상층액을 수득한다. 상기 상층액은 엑소좀을 포함한다. 상기 원심분리는 10,000 xg에서 수행한다.Then, the juice from the cow was centrifuged to obtain a supernatant. The supernatant contains exosomes. The centrifugation is performed at 10,000 xg.
다음, 상층액을 동결건조하여 동결건조물을 제조한다. 동결건조기를 이용하여 수행할 수 있다.Next, the supernatant is freeze-dried to prepare a lyophilisate. It can be carried out using a lyophilizer.
다음, 동결건조물에 폴리에틸렌글리콜염, 특히 폴리에틸렌글리콜 및 염으로는 덱스트란(dextran)을 사용하여 수성 2상계를 형성한다. 엑소좀을 효율적으로 분리 정제하기 위해 수성 2상분배법을 사용한다.Next, an aqueous two-phase system is formed by using a polyethylene glycol salt, particularly polyethylene glycol and dextran as the salt, in the lyophilized product. An aqueous two-phase partitioning method is used to efficiently separate and purify exosomes.
상기 폴리에틸렌글리콜은 중량평균분자량이 10,000-35,000인 것을 1-15 중량%, 바람직하게는 2-5 중량% 사용하고 덱스트란은 중량평균분자량이 300,000-650,000인 것을 1-8 중량%, 바람직하게는 1-3 중량% 사용하는 것을 특징으로 한다. 상기 폴리에틸렌글리콜과 덱스트란이 3.3 중량% : 1.7 중량%의 농도비율로 사용되는 경우에 엑소좀의 수율이 가장 높고, 안정성이 가장 우수하므로 더욱 바람직하다.The polyethylene glycol has a weight average molecular weight of 10,000-35,000 and is used at 1-15% by weight, preferably 2-5% by weight, and dextran has a weight average molecular weight of 300,000-650,000 at 1-8% by weight, preferably It is characterized by using 1-3% by weight. When the polyethylene glycol and dextran are used in a concentration ratio of 3.3% by weight: 1.7% by weight, the yield of exosomes is the highest and the stability is the best, so it is more preferable.
다음, 수성 2상계 내 엑소좀이 농축된 하층액을 수득한다.Next, a lower layer in which the exosomes in the aqueous two-phase system are concentrated is obtained.
다음으로, 본 발명에 따른 화장료 조성물의 제조방법은 올리고 히알루론산을 제조하는 단계를 포함한다. 상기 올리고 히알루론산을 제조하는 단계는, 중량평균분자량이 1,000-3,000인 히알루론산 63-67 중량부 및 중량평균분자량이 15,000-20,000인 히알루론산 33-37 중량부를 혼합하여 혼합물을 제조하는 단계; 및 상기 혼합물을 33-37℃의 온도에서 2-6시간 동안 교반하는 단계;를 포함한다.Next, the manufacturing method of the cosmetic composition according to the present invention includes preparing oligo hyaluronic acid. The preparing of the oligo hyaluronic acid may include preparing a mixture by mixing 63 to 67 parts by weight of hyaluronic acid having a weight average molecular weight of 1,000 to 3,000 and 33 to 37 parts by weight of hyaluronic acid having a weight average molecular weight of 15,000 to 20,000; and stirring the mixture at a temperature of 33-37° C. for 2-6 hours.
상기 올리고 히알루론산은 중량평균분자량이 1,000-3,000인 초저분자 히알루론산 63-67 중량부, 바람직하게는 64-66 중량부 및 중량평균분자량이 15,000-20,000인 저분자 히알루론산 33-37 중량부, 바람직하게는 34-36 중량부를 혼합한다.The oligo hyaluronic acid is 63-67 parts by weight of ultra-low molecular weight hyaluronic acid having a weight average molecular weight of 1,000-3,000, preferably 64-66 parts by weight and 33-37 parts by weight of low molecular weight hyaluronic acid having a weight average molecular weight of 15,000-20,000, preferably Preferably 34-36 parts by weight are mixed.
상기 혼합물을 34-36℃의 온도에서 3-5시간 동안 교반하여 안정화시키는 것이 더욱 바람직하다.More preferably, the mixture is stabilized by stirring at a temperature of 34-36° C. for 3-5 hours.
다음으로, 본 발명에 따른 화장료 조성물의 제조방법은 소자 추출물 및 시계초 추출물을 제조하는 단계를 포함한다. 상기 소자 추출물 및 시계초 추출물을 제조하는 단계는, 소자 및 시계초를 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 소자분말 및 시계초분말을 준비하는 단계; 상기 소자분말 및 시계초분말을 1:1의 중량비율로 혼합하여 제1 혼합분말을 준비하는 단계; 상기 제1 혼합분말 및 물을 혼합하고, 교반을 수행하며 95-105℃의 온도에서 10-14시간 동안 1차 열수추출을 수행하는 단계; 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제1 추출물을 제조하는 단계; 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 남은 고형물을 다시 물과 혼합하고, 135-145℃의 온도에서 4-8시간 동안 2차 열수추출을 수행하는 단계; 2차 열수추출 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제2 추출물을 제조하는 단계; 및 상기 제1 추출물 및 제2 추출물을 혼합하는 단계;를 포함한다.Next, the manufacturing method of the cosmetic composition according to the present invention includes the step of preparing a soja extract and a passion fruit extract. The steps of preparing the elemental extract and clockwork extract include: preparing elemental powder and clockwise powder by washing elements and clockwork, cutting them into appropriate sizes, lyophilizing, and pulverizing; preparing a first mixed powder by mixing the element powder and clock start powder in a weight ratio of 1:1; mixing the first mixed powder and water, stirring, and performing primary hot water extraction at a temperature of 95-105° C. for 10-14 hours; After performing the first hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a first extract; After performing the first hot water extraction, filtering with a filter paper, mixing the remaining solids with water again, and performing a second hot water extraction at a temperature of 135-145 ° C. for 4-8 hours; After the second hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a second extract; and mixing the first extract and the second extract.
상기 소자 추출물 및 시계초 추출물은 다단계 열수추출 공정을 이용하여 추출함으로써 소자 및 시계초에 포함되는 유효성분을 효과적으로 추출할 수 있다. 또한, 이러한 추출물을 화장료에 적용하는 경우 인체에 해롭지 않다.The element extract and the passion fruit extract can effectively extract the active ingredients contained in the element and passion fruit extract by extracting using a multi-step hot water extraction process. In addition, when these extracts are applied to cosmetics, they are not harmful to the human body.
다음으로, 본 발명에 따른 화장료 조성물의 제조방법은 오미자씨 오일 및 복숭아씨 오일을 제조하는 단계를 포함한다. 상기 오미자씨 오일 및 복숭아씨 오일을 제조하는 단계는, 오미자 및 복숭아를 파쇄한 후 세척하여 오미자씨 및 복숭아씨를 분리하는 단계; 분리된 오미자씨 및 복숭아씨를 분쇄하여 오미자씨분말 및 복숭아씨분말을 준비하는 단계; 상기 오미자씨분말 및 복숭아씨분말을 1:1의 중량비율로 혼합하여 제2 혼합분말을 준비하는 단계; 상기 제2 혼합분말 및 헥산을 혼합하고, 28-32℃의 온도에서 2-4일 동안 교반하여 추출물을 제조하는 단계; 및 상기 추출물을 여과하고 감압농축한 후 원심분리하여 이물질을 제거한 후, 감압증류하는 단계;를 포함한다. 상기 감압증류는 일반적으로 오일을 제조하기 위한 공정을 적용한다.Next, the manufacturing method of the cosmetic composition according to the present invention includes preparing Schisandra seed oil and peach seed oil. The step of preparing the Schisandra seed oil and peach seed oil is to separate Schisandra seeds and peach seeds by crushing and washing Schisandra seeds and peaches; Preparing Schisandra seed powder and peach seed powder by grinding the separated Schizandra seeds and peach seeds; Preparing a second mixed powder by mixing the Schizandra seed powder and peach seed powder in a weight ratio of 1: 1; Preparing an extract by mixing the second mixed powder and hexane and stirring at a temperature of 28-32 ° C for 2-4 days; And filtering the extract, concentrating under reduced pressure, centrifuging to remove foreign substances, and then distilling under reduced pressure. The vacuum distillation generally applies a process for producing oil.
다음으로, 본 발명에 따른 화장료 조성물의 제조방법은 호박씨 발효추출물을 제조하는 단계를 포함한다. 상기 호박씨 발효추출물을 제조하는 단계는, 호박씨를 끓인 물로 세척하고, 분쇄하여 호박씨분말을 준비하는 단계; 호박씨 및 물을 혼합하여 혼합물을 제조하고, 혼합물에 효모균으로 사카로미케스 케레비시아이(Saccharomyces cerevisiae)를 첨가한 후 38-42℃의 온도에서 34-38시간 동안 발효시키는 단계; 발효된 혼합물 및 부틸렌글리콜을 혼합하고, 28-32℃의 온도에서 4-6일 동안 교반하여 추출물을 제조하는 단계; 및 상기 추출물을 여과 및 감압농축한 후 동결건조하는 단계;를 포함한다. 상기 발효를 효모균 중에서 사카로미케스 케레비시아이를 적용하여 효과적으로 발효시킬 수 있다.Next, the manufacturing method of the cosmetic composition according to the present invention includes preparing a fermented pumpkin seed extract. Preparing the pumpkin seed fermented extract includes preparing pumpkin seed powder by washing and pulverizing pumpkin seeds with boiled water; Mixing pumpkin seeds and water to prepare a mixture, adding Saccharomyces cerevisiae as a yeast to the mixture, and then fermenting at a temperature of 38-42 ° C. for 34-38 hours; Preparing an extract by mixing the fermented mixture and butylene glycol and stirring at a temperature of 28-32 ° C. for 4-6 days; and lyophilizing the extract after filtering and concentrating under reduced pressure. The fermentation can be effectively fermented by applying Saccharomyces cerevisiae among yeasts.
다음으로, 본 발명에 따른 화장료 조성물의 제조방법은 상기 수삼 유래 엑소좀 1-5 중량부, 상기 소자 유래 엑소좀 1-5 중량부, 아세틸헥사펩타이드-8 0.5-4 중량부, 상기 올리고 히알루론산 4-8 중량부, 피토콜라겐 1-5 중량부, 상기 소자 추출물 0.3-2 중량부, 상기 시계초 추출물 0.3-2 중량부, 스쿠알란 0.5-4 중량부, 펜틸렌글라이콜 0.5-4 중량부, 나이아신아마이드 0.1-1 중량부, 세테아릴알코올 0.5-4 중량부, 글리세릴스테아레이트 0.5-4 중량부, 스테아릴알코올 0.1-1 중량부, 프로판다이올 0.5-4 중량부, 카보머 1.5-3.5 중량부, 리모넨 0.5-4 중량부, 리날룰 0.5-4 중량부 및 당근수 23-27 중량부를 혼합하고, 48-52℃의 온도에서 50-70분 동안 교반하여 제1 혼합물을 제조하는 단계를 포함한다.Next, the manufacturing method of the cosmetic composition according to the present invention includes 1-5 parts by weight of the fresh ginseng-derived exosomes, 1-5 parts by weight of the device-derived exosomes, 0.5-4 parts by weight of acetyl hexapeptide-8, and the oligo hyaluronic acid. 4-8 parts by weight, phytocollagen 1-5 parts by weight, 0.3-2 parts by weight of the soybean extract, 0.3-2 parts by weight of the passion fruit extract, 0.5-4 parts by weight of squalane, 0.5-4 parts by weight of pentylene glycol, 0.1-1 part by weight of niacinamide, 0.5-4 parts by weight of cetearyl alcohol, 0.5-4 parts by weight of glyceryl stearate, 0.1-1 part by weight of stearyl alcohol, 0.5-4 parts by weight of propanediol, 1.5-4 parts by weight of carbomer Preparing a first mixture by mixing 3.5 parts by weight, 0.5-4 parts by weight of limonene, 0.5-4 parts by weight of linalool and 23-27 parts by weight of carrot water and stirring at a temperature of 48-52 ° C. for 50-70 minutes includes
상기 단계에서는 수삼 유래 엑소좀 등과 과량의 당근수를 이용하여 제1 혼합물을 제조하되 특정 온도 및 공정 시간 동안 교반하여 제조한다.In the above step, a first mixture is prepared using fresh ginseng-derived exosomes and an excess of carrot water, but is prepared by stirring for a specific temperature and processing time.
다음으로, 본 발명에 따른 화장료 조성물의 제조방법은 세라마이드 0.5-4 중량부, 상기 오미자씨 오일 0.3-2 중량부, 상기 복숭아씨 오일 0.3-2 중량부, 상기 호박씨 발효추출물 0.3-2 중량부, 은행나무잎 추출물 0.3-2 중량부, 아보카도 추출물 0.3-2 중량부, 글리세린 1-5 중량부, 폴리글리세릴-6스테아레이트 0.1-1 중량부, 폴리글리세릴-2스테아레이트 0.1-1 중량부, 부틸렌글라이콜 2-6 중량부, 카보머 1.5-3.5 중량부, 세틸에틸헥사노에이트 0.5-4 중량부, 솔비톨 0.1-1 중량부, 트로메타민 0.1-1 중량부, 폴리글리세릴-6베헤네이트 0.1-1 중량부, 1,2-헥산다이올 0.1-1 중량부 및 당근수 13-17 중량부를 혼합하고, 78-82℃의 온도에서 20-40분 동안 교반하여 제2 혼합물을 제조하는 단계를 포함한다.Next, the method for producing a cosmetic composition according to the present invention includes 0.5-4 parts by weight of ceramide, 0.3-2 parts by weight of the Schisandra seed oil, 0.3-2 parts by weight of the peach seed oil, 0.3-2 parts by weight of the fermented pumpkin seed extract, 0.3-2 parts by weight of ginkgo leaf extract, 0.3-2 parts by weight of avocado extract, 1-5 parts by weight of glycerin, 0.1-1 part by weight of polyglyceryl-6 stearate, 0.1-1 part by weight of polyglyceryl-2 stearate , butylene glycol 2-6 parts by weight, carbomer 1.5-3.5 parts by weight, cetylethylhexanoate 0.5-4 parts by weight, sorbitol 0.1-1 parts by weight, tromethamine 0.1-1 parts by weight, polyglyceryl- 0.1-1 part by weight of 6 behenate, 0.1-1 part by weight of 1,2-hexanediol, and 13-17 parts by weight of carrots were mixed and stirred at a temperature of 78-82 ° C. for 20-40 minutes to obtain a second mixture. It includes manufacturing steps.
상기 단계에서는 세라마이드, 오미자씨 오일 등, 미량의 당근수를 이용하여 제2 혼합물을 제조하되 특정 온도 및 공정 시간 동안 교반하여 제조한다.In the above step, a second mixture is prepared using a small amount of carrot water such as ceramide, Schisandra seed oil, etc., but is prepared by stirring for a specific temperature and process time.
다음으로, 본 발명에 따른 화장료 조성물의 제조방법은 상기 제1 혼합물 및 상기 제2 혼합물을 혼합하고, 63-67℃의 온도에서 20-40분 동안 교반하는 단계를 포함한다.Next, the manufacturing method of the cosmetic composition according to the present invention includes mixing the first mixture and the second mixture and stirring at a temperature of 63-67 ° C. for 20-40 minutes.
상기 공정을 통해 최종적으로 화장료 조성물을 제조한다.Through the above process, a cosmetic composition is finally prepared.
이하, 본 발명을 하기의 실시예에 의해 보다 상세하게 설명한다.Hereinafter, the present invention will be described in more detail by the following examples.
단, 하기 실시예는 본 발명의 내용을 예시하는 것일 뿐 발명의 범위가 실시예 및 실험예에 의해 한정되는 것은 아니다.However, the following examples are merely illustrative of the content of the present invention, but the scope of the invention is not limited by the examples and experimental examples.
<제조예 1> 수삼 엑소좀의 제조<Preparation Example 1> Preparation of fresh ginseng exosomes
수삼을 세척하여 초고압기에 넣고 20℃의 온도에서 150 MPa의 압력으로 5분 동안 전처리하였다. 전처리된 수삼을 착즙기를 이용해 착즙하여 수삼 착즙액을 제조하였다. 상기 수삼 착즙액을 10,000xg에서 10분 동안 원심분리한 후, 상층액을 수득하였다. 상기 상층액을 -50℃의 온도에서 8시간 동안 동결건조하여 동결건조물을 제조하였다. 상기 동결건조물에 중량평균분자량 10,000-35,000인 폴리에틸렌글리콜 3.3 중량%와 중량평균분자량 400,000-500,000인 덱스트란을 1.7 중량%의 농도인 수용액을 사용하여 수성 2상계를 형성하였다. 상기 수성 2상계 내 엑소좀이 농축된 하층액을 수득하여 수삼 유래 엑소좀을 제조하였다.Fresh ginseng was washed and placed in an ultra-high pressure machine and pretreated at 20°C and 150 MPa pressure for 5 minutes. The pretreated fresh ginseng was extracted using a juicer to prepare fresh ginseng juice. After the fresh ginseng juice was centrifuged at 10,000xg for 10 minutes, the supernatant was obtained. The supernatant was freeze-dried at -50 °C for 8 hours to prepare a lyophilisate. An aqueous two-phase system was formed using an aqueous solution containing 3.3% by weight of polyethylene glycol having a weight average molecular weight of 10,000 to 35,000 and dextran having a weight average molecular weight of 400,000 to 500,000 at a concentration of 1.7% by weight to the lyophilisate. Fresh ginseng-derived exosomes were prepared by obtaining a lower layer in which exosomes were concentrated in the aqueous two-phase system.
<제조예 2> 소자 엑소좀의 제조<Preparation Example 2> Preparation of device exosomes
소자를 세척하여 초고압기에 넣고 30℃의 온도에서 300 MPa의 압력으로 3분 동안 전처리하였다. 전처리된 소자를 착즙기를 이용해 착즙하여 소자 착즙액을 제조하였다. 상기 소자 착즙액을 10,000xg에서 10분 동안 원심분리한 후, 상층액을 수득하였다. 상기 상층액을 -50℃의 온도에서 8시간 동안 동결건조하여 동결건조물을 제조하였다. 상기 동결건조물에 중량평균분자량 10,000-35,000인 폴리에틸렌글리콜 3.3 중량%와 중량평균분자량 400,000-500,000인 덱스트란을 1.7 중량%의 농도인 수용액을 사용하여 수성 2상계를 형성하였다. 상기 수성 2상계 내 엑소좀이 농축된 하층액을 수득하여 소자 유래 엑소좀을 제조하였다.After cleaning the device, it was placed in an ultra-high pressure machine and pretreated at a temperature of 30° C. and a pressure of 300 MPa for 3 minutes. The pretreated element was juiced using a juicer to prepare a element juice. After centrifuging the soybean juice at 10,000xg for 10 minutes, a supernatant was obtained. The supernatant was freeze-dried at -50 °C for 8 hours to prepare a lyophilisate. An aqueous two-phase system was formed using an aqueous solution containing 3.3% by weight of polyethylene glycol having a weight average molecular weight of 10,000 to 35,000 and 1.7% by weight of dextran having a weight average molecular weight of 400,000 to 500,000 to the lyophilisate. A lower layer in which exosomes were concentrated in the aqueous two-phase system was obtained to prepare device-derived exosomes.
<제조예 3> 올리고 히알루론산의 제조<Preparation Example 3> Preparation of oligo hyaluronic acid
중량평균분자량이 1,000-3,000인 히알루론산 65 중량부 및 중량평균분자량이 15,000-20,000인 히알루론산 35 중량부를 혼합하고, 35℃의 온도에서 4시간 동안 교반하여 올리고 히알루론산을 제조하였다.Oligo hyaluronic acid was prepared by mixing 65 parts by weight of hyaluronic acid having a weight average molecular weight of 1,000-3,000 and 35 parts by weight of hyaluronic acid having a weight average molecular weight of 15,000-20,000, and stirring at a temperature of 35 ° C. for 4 hours.
<제조예 4> 소자 추출물 및 시계초 추출물의 제조<Preparation Example 4> Preparation of soja extract and passion fruit extract
소자 및 시계초를 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 소자분말 및 시계초분말을 준비하였다. 상기 소자분말 및 시계초분말을 1:1의 중량비율로 혼합하여 제1 혼합분말을 준비하였다. 상기 제1 혼합분말 10 g 및 물 90 g을 혼합하고, 교반을 수행하며 100℃의 온도에서 12시간 동안 1차 열수추출을 수행하였다. 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제1 추출물을 제조하였다. 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 남은 고형물을 다시 물과 혼합하고, 140℃의 온도에서 6시간 동안 2차 열수추출을 수행하였다. 2차 열수추출 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제2 추출물을 제조하였다. 상기 제1 추출물 및 제2 추출물을 혼합하여 소자 추출물 및 시계초 추출물을 제조하였다.The element and clock grass were washed, cut into appropriate sizes, lyophilized, and pulverized to prepare element powder and clock grass powder. A first mixed powder was prepared by mixing the element powder and clock start powder in a weight ratio of 1:1. 10 g of the first mixed powder and 90 g of water were mixed, and a first hot water extraction was performed at a temperature of 100° C. for 12 hours while stirring. After the first hot water extraction was performed, the filtrate was filtered with a filter paper and the filtrate was freeze-dried to prepare a first extract. After performing the first hot water extraction, the mixture was filtered with a filter paper, the remaining solids were mixed with water again, and the second hot water extraction was performed at a temperature of 140° C. for 6 hours. After the second hot water extraction, the mixture was filtered with a filter paper, and the filtrate was freeze-dried to prepare a second extract. Soja extract and passion fruit extract were prepared by mixing the first extract and the second extract.
<제조예 5> 오미자씨 오일 및 복숭아씨 오일의 제조<Preparation Example 5> Preparation of Schisandra Seed Oil and Peach Seed Oil
오미자 및 복숭아를 파쇄한 후 세척하여 오미자씨 및 복숭아씨를 분리하고, 분리된 오미자씨 및 복숭아씨를 분쇄하여 오미자씨분말 및 복숭아씨분말을 준비하였다. 상기 오미자씨분말 및 복숭아씨분말을 1:1의 중량비율로 혼합하여 제2 혼합분말을 준비하였다. 상기 제2 혼합분말 10 g 및 헥산 60 g을 혼합하고, 30℃의 온도에서 3일 동안 교반하여 추출물을 제조하였다. 이를 여과하고 감압 농축한 후 원심분리하여 이물질을 제거하는 과정을 반복하였다. 최후 감압 증류를 반복함으로써 잔류용매인 헥산을 0.1 ppm 이하로 제거하여 이를 통해 오미자씨 오일 및 복숭아씨 오일을 제조하였다.Schisandra seeds and peach seeds were crushed and washed to separate Schisandra seeds and peach seeds, and the separated Schisandra seeds and peach seeds were pulverized to prepare Schisandra seed powder and peach seed powder. A second mixed powder was prepared by mixing the Schisandra seed powder and peach seed powder in a weight ratio of 1:1. An extract was prepared by mixing 10 g of the second mixed powder and 60 g of hexane, and stirring at a temperature of 30° C. for 3 days. After filtering and concentrating under reduced pressure, the process of centrifuging to remove foreign substances was repeated. By repeating the last vacuum distillation, the residual solvent, hexane, was removed to less than 0.1 ppm, thereby preparing Schizandra seed oil and peach seed oil.
<제조예 6> 호박씨 발효추출물의 제조<Preparation Example 6> Preparation of pumpkin seed fermented extract
호박씨를 끓인 물로 3회 세척하고, 분쇄하여 호박씨분말을 준비하였다. 호박씨분말 10 g 및 물 300 g을 혼합하여 혼합물을 제조하고, 혼합물에 효모균으로 사카로미케스 케레비시아이(Saccharomyces cerevisiae)를 0.3 g 첨가한 후 40℃의 온도에서 36시간 동안 발효시켰다. 발효된 혼합물에 부틸렌글리콜 300 g을 혼합하고, 30℃의 온도에서 5일 동안 교반하여 추출물을 제조하였다. 상기 추출물을 여과 및 감압농축한 후 동결건조하여 호박씨 발효추출물을 제조하였다.Pumpkin seeds were washed three times with boiled water and ground to prepare pumpkin seed powder. A mixture was prepared by mixing 10 g of pumpkin seed powder and 300 g of water, and after adding 0.3 g of Saccharomyces cerevisiae as a yeast to the mixture, the mixture was fermented at a temperature of 40° C. for 36 hours. An extract was prepared by mixing 300 g of butylene glycol with the fermented mixture and stirring at a temperature of 30° C. for 5 days. The extract was filtered, concentrated under reduced pressure, and lyophilized to prepare a fermented pumpkin seed extract.
<실시예 1-3 및 비교예 1-2> 화장료 조성물의 제조<Example 1-3 and Comparative Example 1-2> Preparation of cosmetic composition
상기 제조예 1에서 제조된 수삼 유래 엑소좀, 상기 제조예 2에서 제조된 소자 유래 엑소좀, 아세틸헥사펩타이드-8, 상기 제조예 3에서 제조된 올리고 히알루론산, 초저분자 히알루론산 또는 저분자 히알루론산, 피토콜라겐, 상기 제조예 4에서 제조된 소자 추출물 및 시계초 추출물, 스쿠알란, 펜틸렌글라이콜, 나이아신아마이드, 세테아릴알코올, 글리세릴스테아레이트, 스테아릴알코올, 프로판다이올, 카보머, 리모넨, 리날룰 및 당근수를 혼합하고, 50℃의 온도에서 60분 동안 교반하여 제1 혼합물을 제조하였다.Fresh ginseng-derived exosomes prepared in Preparation Example 1, Soja-derived exosomes prepared in Preparation Example 2, acetylhexapeptide-8, oligo hyaluronic acid, ultra-low molecular weight hyaluronic acid or low molecular weight hyaluronic acid prepared in Preparation Example 3, Phytocollagen, soybean extract and passion fruit extract prepared in Preparation Example 4, squalane, pentylene glycol, niacinamide, cetearyl alcohol, glyceryl stearate, stearyl alcohol, propanediol, carbomer, limonene, A first mixture was prepared by mixing linalool and carrot water and stirring at a temperature of 50° C. for 60 minutes.
세라마이드, 상기 제조예 5에서 제조된 오미자씨 오일 및 복숭아씨 오일, 상기 제조예 6에서 제조된 호박씨 발효추출물, 은행나무잎 추출물, 아보카도 추출물, 글리세린, 폴리글리세릴-6스테아레이트, 폴리글리세릴-2스테아레이트, 부틸렌글라이콜, 카보머, 세틸에틸헥사노에이트, 솔비톨, 트로메타민, 폴리글리세릴-6베헤네이트, 1,2-헥산다이올 및 당근수를 혼합하고, 80℃의 온도에서 30분 동안 교반하여 제2 혼합물을 제조하였다.Ceramide, Schizandra seed oil and peach seed oil prepared in Preparation Example 5, pumpkin seed fermented extract prepared in Preparation Example 6, ginkgo biloba leaf extract, avocado extract, glycerin, polyglyceryl-6 stearate, polyglyceryl- 2 Stearate, butylene glycol, carbomer, cetylethylhexanoate, sorbitol, tromethamine, polyglyceryl-6behenate, 1,2-hexanediol and carrot water were mixed, and the temperature was 80 ° C. was stirred for 30 minutes to prepare a second mixture.
상기 제1 혼합물 및 상기 제2 혼합물을 혼합하고, 65℃의 온도에서 30분 동안 교반하여 화장료 조성물을 제조하였다.The first mixture and the second mixture were mixed and stirred at a temperature of 65° C. for 30 minutes to prepare a cosmetic composition.
상기 화장료 조성물의 조성은 하기 표 1에 나타내었다.The composition of the cosmetic composition is shown in Table 1 below.
<실험예 1> <Experimental Example 1>
상기 실시예 1-3 및 비교예 1-2에 의해 제조된 본 발명의 화장료 조성물에 대한 시험대상자의 피부개선 유효성 효과, 민감성 피부사용 적합성 및 기호도를 확인하기 위하여 설문평가를 수행하였다. 시험대상자는 총 5점 척도(5: 매우 만족, 4: 만족, 3: 보통, 2: 불만족, 1: 매우 불만족)로 직접 설문평가를 작성하였다. 그 결과를 하기 표 2에 나타내었다.Questionnaire evaluation was conducted to confirm the efficacy of the cosmetic composition of the present invention prepared in Example 1-3 and Comparative Example 1-2 for improving the skin of test subjects, suitability for use on sensitive skin, and preference. The subjects completed the questionnaire evaluation directly on a total of 5-point scale (5: very satisfied, 4: satisfied, 3: normal, 2: dissatisfied, 1: very dissatisfied). The results are shown in Table 2 below.
사용 적합성sensitive skin
suitability for use
상기 표 2에 나타낸 바와 같이, 본 발명에 따른 화장료 조성물이 보습 효과가 우수하고 피부 장벽개선에 효과적인 것을 확인할 수 있었다. 특히 실시예 1의 경우 매우 우수한 경향을 나타냄을 확인할 수 있었다.As shown in Table 2, it was confirmed that the cosmetic composition according to the present invention has excellent moisturizing effect and is effective in improving the skin barrier. In particular, it was confirmed that Example 1 showed a very good tendency.
Claims (3)
상기 올리고 히알루론산은 중량평균분자량이 1,000-3,000인 히알루론산 63-67 중량부 및 중량평균분자량이 15,000-20,000인 히알루론산 33-37 중량부를 포함하는 화장료 조성물.1-5 parts by weight of fresh ginseng-derived exosomes, 1-5 parts by weight of Soja-derived exosomes, 0.5-4 parts by weight of acetyl hexapeptide-8, 4-8 parts by weight of oligo hyaluronic acid, 1-5 parts by weight of phytocollagen, 0.5 parts by weight of ceramide -4 parts by weight, Soja extract 0.3-2 parts by weight, Passionflower extract 0.3-2 parts by weight, Schizandra seed oil 0.3-2 parts by weight, Peach seed oil 0.3-2 parts by weight, Pumpkin seed fermented extract 0.3-2 parts by weight, Ginkgo biloba Tree leaf extract 0.3-2 parts by weight, avocado extract 0.3-2 parts by weight, glycerin 1-5 parts by weight, squalane 0.5-4 parts by weight, pentylene glycol 0.5-4 parts by weight, niacinamide 0.1-1 parts by weight, poly 0.1-1 part by weight of glyceryl-6 stearate, 0.1-1 part by weight of polyglyceryl-2 stearate, 2-6 parts by weight of butylene glycol, 0.5-4 part by weight of cetearyl alcohol, 0.5 part by weight of glyceryl stearate -4 parts by weight, stearyl alcohol 0.1-1 parts by weight, propanediol 0.5-4 parts by weight, carbomer 3-7 parts by weight, cetylethylhexanoate 0.5-4 parts by weight, sorbitol 0.1-1 parts by weight, Tro 0.1-1 part by weight of metamine, 0.1-1 part by weight of polyglyceryl-6behenate, 0.1-1 part by weight of 1,2-hexanediol, 0.5-4 part by weight of limonene, 0.5-4 part by weight of linalool and carrots 38-42 parts by weight;
The oligo hyaluronic acid is a cosmetic composition comprising 63-67 parts by weight of hyaluronic acid having a weight average molecular weight of 1,000-3,000 and 33-37 parts by weight of hyaluronic acid having a weight average molecular weight of 15,000-20,000.
소자를 세척하여 28-32℃의 온도에서 290-310 MPa의 압력으로 1-5분 동안 전처리하는 단계; 전처리된 소자를 착즙하여 소자 착즙액을 제조하는 단계; 상기 소자 착즙액을 원심분리하여 엑소좀을 포함하는 상층액을 수득하는 단계; 상기 엑소좀을 포함하는 상층액을 동결건조하여 동결건조물을 제조하는 단계; 상기 동결건조물에 폴리에틸렌글리콜/덱스트란을 사용하여 수성 2상계를 형성하는 단계; 및 상기 수성 2상계 내 엑소좀이 농축된 하층액을 수득하는 단계;를 포함하는 소자 유래 엑소좀을 제조하는 단계;
중량평균분자량이 1,000-3,000인 히알루론산 63-67 중량부 및 중량평균분자량이 15,000-20,000인 히알루론산 33-37 중량부를 혼합하여 혼합물을 제조하는 단계; 및 상기 혼합물을 33-37℃의 온도에서 2-6시간 동안 교반하는 단계;를 포함하는 올리고 히알루론산을 제조하는 단계;
소자 및 시계초를 세척하고, 적절한 크기로 자른 뒤 동결건조시키고, 분쇄하여 소자분말 및 시계초분말을 준비하는 단계; 상기 소자분말 및 시계초분말을 1:1의 중량비율로 혼합하여 제1 혼합분말을 준비하는 단계; 상기 제1 혼합분말 및 물을 혼합하고, 교반을 수행하며 95-105℃의 온도에서 10-14시간 동안 1차 열수추출을 수행하는 단계; 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제1 추출물을 제조하는 단계; 1차 열수추출을 수행하고난 후, 여과지로 여과한 다음 남은 고형물을 다시 물과 혼합하고, 135-145℃의 온도에서 4-8시간 동안 2차 열수추출을 수행하는 단계; 2차 열수추출 후, 여과지로 여과한 다음 그 여액을 동결건조하여 제2 추출물을 제조하는 단계; 및 상기 제1 추출물 및 제2 추출물을 혼합하는 단계;를 포함하는 소자 추출물 및 시계초 추출물을 제조하는 단계;
오미자 및 복숭아를 파쇄한 후 세척하여 오미자씨 및 복숭아씨를 분리하는 단계; 분리된 오미자씨 및 복숭아씨를 분쇄하여 오미자씨분말 및 복숭아씨분말을 준비하는 단계; 상기 오미자씨분말 및 복숭아씨분말을 1:1의 중량비율로 혼합하여 제2 혼합분말을 준비하는 단계; 상기 제2 혼합분말 및 헥산을 혼합하고, 28-32℃의 온도에서 2-4일 동안 교반하여 추출물을 제조하는 단계; 및 상기 추출물을 여과하고 감압농축한 후 원심분리하여 이물질을 제거한 후, 감압증류하는 단계;를 포함하는 오미자씨 오일 및 복숭아씨 오일을 제조하는 단계;
호박씨를 끓인 물로 세척하고, 분쇄하여 호박씨분말을 준비하는 단계; 호박씨분말 및 물을 혼합하여 혼합물을 제조하고, 혼합물에 효모균으로 사카로미케스 케레비시아이(Saccharomyces cerevisiae)를 첨가한 후 38-42℃의 온도에서 34-38시간 동안 발효시키는 단계; 발효된 혼합물 및 부틸렌글리콜을 혼합하고, 28-32℃의 온도에서 4-6일 동안 교반하여 추출물을 제조하는 단계; 및 상기 추출물을 여과 및 감압농축한 후 동결건조하는 단계;를 포함하는 호박씨 발효추출물을 제조하는 단계;
상기 수삼 유래 엑소좀 1-5 중량부, 상기 소자 유래 엑소좀 1-5 중량부, 아세틸헥사펩타이드-8 0.5-4 중량부, 상기 올리고 히알루론산 4-8 중량부, 피토콜라겐 1-5 중량부, 상기 소자 추출물 및 시계초 추출물 0.6-4 중량부, 스쿠알란 0.5-4 중량부, 펜틸렌글라이콜 0.5-4 중량부, 나이아신아마이드 0.1-1 중량부, 세테아릴알코올 0.5-4 중량부, 글리세릴스테아레이트 0.5-4 중량부, 스테아릴알코올 0.1-1 중량부, 프로판다이올 0.5-4 중량부, 카보머 1.5-3.5 중량부, 리모넨 0.5-4 중량부, 리날룰 0.5-4 중량부 및 당근수 23-27 중량부를 혼합하고, 48-52℃의 온도에서 50-70분 동안 교반하여 제1 혼합물을 제조하는 단계;
세라마이드 0.5-4 중량부, 상기 오미자씨 오일 및 복숭아씨 오일 0.6-4 중량부, 상기 호박씨 발효추출물 0.3-2 중량부, 은행나무잎 추출물 0.3-2 중량부, 아보카도 추출물 0.3-2 중량부, 글리세린 1-5 중량부, 폴리글리세릴-6스테아레이트 0.1-1 중량부, 폴리글리세릴-2스테아레이트 0.1-1 중량부, 부틸렌글라이콜 2-6 중량부, 카보머 1.5-3.5 중량부, 세틸에틸헥사노에이트 0.5-4 중량부, 솔비톨 0.1-1 중량부, 트로메타민 0.1-1 중량부, 폴리글리세릴-6베헤네이트 0.1-1 중량부, 1,2-헥산다이올 0.1-1 중량부 및 당근수 13-17 중량부를 혼합하고, 78-82℃의 온도에서 20-40분 동안 교반하여 제2 혼합물을 제조하는 단계; 및
상기 제1 혼합물 및 상기 제2 혼합물을 혼합하고, 63-67℃의 온도에서 20-40분 동안 교반하는 단계;를 포함하는 화장료 조성물의 제조방법.Washing fresh ginseng and pre-treating it at a temperature of 18-22°C and a pressure of 140-160 MPa for 3-7 minutes; Preparing fresh ginseng juice by extracting pretreated fresh ginseng; Obtaining a supernatant containing exosomes by centrifuging the fresh ginseng juice; preparing a lyophilisate by freeze-drying the supernatant containing the exosomes; forming an aqueous two-phase system using polyethylene glycol/dextran in the lyophilisate; and obtaining a lower layer in which exosomes are concentrated in the aqueous two-phase system; preparing fresh ginseng-derived exosomes;
washing the device and pre-treating it at a temperature of 28-32° C. and a pressure of 290-310 MPa for 1-5 minutes; Preparing a device juice by extracting the pretreated device; Obtaining a supernatant containing exosomes by centrifuging the juice from the cow; preparing a lyophilisate by freeze-drying the supernatant containing the exosomes; forming an aqueous two-phase system using polyethylene glycol/dextran in the lyophilisate; and obtaining an exosome-enriched lower layer in the aqueous two-phase system; preparing a device-derived exosome comprising;
Preparing a mixture by mixing 63-67 parts by weight of hyaluronic acid having a weight average molecular weight of 1,000-3,000 and 33-37 parts by weight of hyaluronic acid having a weight average molecular weight of 15,000-20,000; and stirring the mixture at a temperature of 33-37° C. for 2-6 hours; preparing an oligo hyaluronic acid comprising;
Washing the elements and clock grass, cutting them into appropriate sizes, freeze-drying them, and pulverizing to prepare element powder and clock grass powder; preparing a first mixed powder by mixing the element powder and clock start powder in a weight ratio of 1:1; mixing the first mixed powder and water, stirring, and performing primary hot water extraction at a temperature of 95-105° C. for 10-14 hours; After performing the first hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a first extract; After performing the first hot water extraction, filtering with a filter paper, mixing the remaining solids with water again, and performing a second hot water extraction at a temperature of 135-145 ° C. for 4-8 hours; After the second hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a second extract; And the step of mixing the first extract and the second extract; preparing a device extract and passion fruit extract containing;
Separating Schizandra seeds and peach seeds by washing after crushing Schizandra chinensis and peaches; Preparing Schisandra seed powder and peach seed powder by grinding the separated Schizandra seeds and peach seeds; Preparing a second mixed powder by mixing the Schizandra seed powder and peach seed powder in a weight ratio of 1: 1; Preparing an extract by mixing the second mixed powder and hexane and stirring at a temperature of 28-32 ° C for 2-4 days; Preparing Omija seed oil and peach seed oil comprising; and filtering and concentrating the extract under reduced pressure, centrifuging to remove foreign matter, and then distilling under reduced pressure;
Preparing pumpkin seed powder by washing and grinding pumpkin seeds with boiled water; Preparing a mixture by mixing pumpkin seed powder and water, adding Saccharomyces cerevisiae as a yeast to the mixture, and then fermenting at a temperature of 38-42 ° C. for 34-38 hours; Preparing an extract by mixing the fermented mixture and butylene glycol and stirring at a temperature of 28-32 ° C. for 4-6 days; Preparing a fermented pumpkin seed extract comprising; and filtering and concentrating the extract under reduced pressure and then freeze-drying it.
1-5 parts by weight of the fresh ginseng-derived exosome, 1-5 parts by weight of the soma-derived exosome, 0.5-4 parts by weight of acetyl hexapeptide-8, 4-8 parts by weight of the oligo hyaluronic acid, 1-5 parts by weight of phytocollagen 0.6-4 parts by weight of the soybean extract and passion fruit extract, 0.5-4 parts by weight of squalane, 0.5-4 parts by weight of pentylene glycol, 0.1-1 part by weight of niacinamide, 0.5-4 parts by weight of cetearyl alcohol, glyceryl 0.5-4 parts by weight of reel stearate, 0.1-1 part by weight of stearyl alcohol, 0.5-4 parts by weight of propanediol, 1.5-3.5 parts by weight of carbomer, 0.5-4 parts by weight of limonene, 0.5-4 parts by weight of linalool, and Mixing 23-27 parts by weight of carrots and stirring at a temperature of 48-52 ° C. for 50-70 minutes to prepare a first mixture;
0.5-4 parts by weight of ceramide, 0.6-4 parts by weight of Schisandra seed oil and peach seed oil, 0.3-2 parts by weight of fermented pumpkin seed extract, 0.3-2 parts by weight of ginkgo leaf extract, 0.3-2 parts by weight of avocado extract, glycerin 1-5 parts by weight, 0.1-1 part by weight of polyglyceryl-6 stearate, 0.1-1 part by weight of polyglyceryl-2 stearate, 2-6 parts by weight of butylene glycol, 1.5-3.5 parts by weight of carbomer, 0.5-4 parts by weight of cetylethylhexanoate, 0.1-1 part by weight of sorbitol, 0.1-1 part by weight of tromethamine, 0.1-1 part by weight of polyglyceryl-6behenate, 0.1-1 part by weight of 1,2-hexanediol preparing a second mixture by mixing parts by weight and 13-17 parts by weight of carrots and stirring at a temperature of 78-82 ° C. for 20-40 minutes; and
Mixing the first mixture and the second mixture, and stirring for 20-40 minutes at a temperature of 63-67 ℃; manufacturing method of a cosmetic composition comprising a.
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| KR102635022B1 (en) * | 2023-05-22 | 2024-02-08 | 주식회사 에이바이오머티리얼즈 | Cosmetic composition containing Perilla Ocymoides exosome, Eucalyptus Globulus exosome, elastic liposome containing azulene and peptides |
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| KR20210027627A (en) * | 2019-08-29 | 2021-03-11 | 도운 | Cosmetic composition with skin recovery and soothing function |
| KR102334486B1 (en) | 2021-05-25 | 2021-12-03 | (주)네오팜 | Cosmetic composition for moisturizing skin and improving skin barrier function |
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- 2022-10-21 KR KR1020220136229A patent/KR102481661B1/en active IP Right Grant
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20210027627A (en) * | 2019-08-29 | 2021-03-11 | 도운 | Cosmetic composition with skin recovery and soothing function |
| KR102334486B1 (en) | 2021-05-25 | 2021-12-03 | (주)네오팜 | Cosmetic composition for moisturizing skin and improving skin barrier function |
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| Title |
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| 네이버 블로그, 가인비책 진 3종세트(2021.10.26.)* * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102635022B1 (en) * | 2023-05-22 | 2024-02-08 | 주식회사 에이바이오머티리얼즈 | Cosmetic composition containing Perilla Ocymoides exosome, Eucalyptus Globulus exosome, elastic liposome containing azulene and peptides |
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