KR102476982B1 - The composition for the weight control including the grains fermentation product and garcinia cambogia - Google Patents

The composition for the weight control including the grains fermentation product and garcinia cambogia Download PDF

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KR102476982B1
KR102476982B1 KR1020220106792A KR20220106792A KR102476982B1 KR 102476982 B1 KR102476982 B1 KR 102476982B1 KR 1020220106792 A KR1020220106792 A KR 1020220106792A KR 20220106792 A KR20220106792 A KR 20220106792A KR 102476982 B1 KR102476982 B1 KR 102476982B1
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weight
barley
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garcinia cambogia
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이영우
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주식회사 위드랩스
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    • AHUMAN NECESSITIES
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23L33/17Amino acids, peptides or proteins
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
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    • A23V2250/00Food ingredients
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    • A23V2250/2118Garcinia cambogia, hydroxycitric acid

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Abstract

The present invention relates to a composition for weight control containing a garcinia cambogia extract, a complex fermented product of barley, and a complex fermented product of adlay, wherein the composition has an excellent weight loss effect by adding enzyme obtained by fermenting grains such as barley and adlay together with soybeans to the garcinia cambogia extract.

Description

곡물 발효물과 가르시니아 캄보지아를 포함하는 체중조절용 조성물{THE COMPOSITION FOR THE WEIGHT CONTROL INCLUDING THE GRAINS FERMENTATION PRODUCT AND GARCINIA CAMBOGIA}Composition for weight control containing fermented grains and Garcinia cambogia

본 발명은 곡물 발효물과 가르시니아 캄보지아 추출물을 포함하는 체중조절용 조성물에 관한 것이다.The present invention relates to a composition for weight control comprising a fermented grain product and an extract of Garcinia cambogia.

체중조절용 식품은 체중의 증가 또는 체중의 감소와 같이 체중조절이 필요한 사람들이 식사의 일부나 전부를 대신할 수 있도록 비타민이나 무기질 등 필요한 영양소를 가감하여 열량을 조절한 식품을 말한다. 최근 식생활 습관의 서구화로 비만 등으로 인한 성인병 발병이 증가하면서, 체중조절을 통해 성인병을 사전에 예방할 수 있는 식품 소재를 이용한 다양한 제형의 건강기능 식품이 시중에 나오고 있다. 식품 소재들 중에서 체중 감소를 위한 대표적인 건강기능 식품 소재로 가르시니아 캄보지아 추출물이 있다. Foods for weight control refer to foods whose calories are adjusted by adding or subtracting necessary nutrients such as vitamins and minerals so that people who need weight control, such as weight gain or weight loss, can replace part or all of their meals. Recently, as the incidence of adult diseases due to obesity increases due to westernization of eating habits, health functional foods of various formulations using food materials that can prevent adult diseases in advance through weight control are coming out on the market. Among food materials, there is a Garcinia cambogia extract as a representative health functional food material for weight loss.

가르시니아 캄보지아 추출물은 물레나물과(Guttiferae)의 식물로서 인도 남서부에서 자생하는 열대 식물로서 열매의 껍질 부위를 사용하며, 껍질에는 기능성 성분인 하이드록시시트로산(hydroxycitric acid, HCA)이 약 10~30% 함유되어 있다. 식품 의약품 안전처에 따르면, 가르시니아 캄보지아 추출물은 기능성분인 HCA의 함량 기준으로 하루 750~2,800mg을 섭취하면 탄수화물이 지방으로 합성되는 것을 억제하여 체지방 감소에 도움을 주는 등 그 기능성을 인정받고 있다. 이처럼 가르시니아 캄보지아 추출물의 생리활성 기능이 입증되면서 체지방의 관리를 원하는 성인들에게 건강기능식품 소재로 권장되고 있다. Garcinia cambogia extract is a plant of the Guttiferae family and is a tropical plant native to southwest India. The peel part of the fruit is used. % contains. According to the Ministry of Food and Drug Safety, Garcinia cambogia extract has been recognized for its functionality, such as helping to reduce body fat by suppressing the synthesis of carbohydrates into fat when 750 to 2,800 mg per day is consumed based on the content of HCA, a functional component. As the physiological activity of Garcinia cambogia extract has been proven, it is recommended as a health functional food ingredient for adults who want to manage body fat.

한편, 가르시니아 캄보지아 추출물은 단독으로 섭취할 경우 하루에 섭취해야하는 기능성 성분의 양이 많아지게되고, 정제, 캡슐 등의 일반적인 건강기능식품 제형에 적합하지 않으며, 물에 타서 마시는 분말 제형 등으로 한정되는 단점이 있다. 그러나 분말 제형의 경우 일정량의 물에 타서 간편히 먹을 수 있는 장점은 있으나, 수중 분산성 내지 용해성이 있는 문제가 있다. 가르시니아 캄보지아 추출물은 용해 시 분말끼리 서로 엉기는 현상이 발생하므로 수중 분산성 내지 용해성이 떨어져 섭치 시 상당한 시간과 노력이 필요하게 되며, 이는 소비자가 제품을 쉽고 간편하게 섭취하는데 있어서 불편함을 야기한다. 이에 섭취가 용이하면서 체중 조절 효과가 우수한 건강기능식품 개발은 지속적으로 요구되어오고 있다. On the other hand, Garcinia cambogia extract increases the amount of functional ingredients that must be consumed per day when consumed alone, is not suitable for general health functional food formulations such as tablets and capsules, and is limited to powder formulations that are dissolved in water and drunk. there is However, in the case of a powder formulation, there is an advantage that it can be easily eaten by mixing in a certain amount of water, but there is a problem of dispersibility or solubility in water. Garcinia cambogia extract is entangled with each other when dissolving, so dispersibility or solubility in water is poor, requiring considerable time and effort when ingesting, which causes inconvenience to consumers in easily and conveniently ingesting the product. Accordingly, there is a continuous demand for the development of health functional foods that are easy to consume and have excellent weight control effects.

한국 공개특허 제10-2021-0035592호Korean Patent Publication No. 10-2021-0035592

본 발명의 목적은 가르시니아 캄보지아 추출물 및 보리복합발효물을 포함하는 체중조절용 식품조성물을 제공하고자 한다. 본 발명에서는 상기 가르시니아 캄보지아 추출물의 함량을 줄이는 대신 보리복합발효물을 첨가함으로써 체중 감량 효과를 높이고자 한다. 또한 보리복합발효물에 첨가제를 포함하여 섭취 용이성을 개선하고자 한다.An object of the present invention is to provide a food composition for weight control comprising a Garcinia cambogia extract and a complex fermented barley product. In the present invention, instead of reducing the content of the Garcinia cambogia extract, it is intended to increase the weight loss effect by adding complex fermented barley. In addition, it is intended to improve the ease of intake by including additives in the barley complex fermentation product.

본 발명의 일 측면은 가르시니아 캄보지아 추출물, 보리복합발효물, 이산화규소, 효소처리스테비아, 정제소금, 및 스테아린산 마그네슘을 포함하는 체중조절용 식품조성물에 관한 것이다. One aspect of the present invention relates to a food composition for weight control comprising a Garcinia cambogia extract, barley complex fermented product, silicon dioxide, enzyme-treated stevia, refined salt, and magnesium stearate.

본 발명에서 “가르시니아 캄보지아 추출물”은 가르시니아 캄보지아(Garcinia cambogia) 열매의 껍질을 물로 추출하고, 이를 건조한 것으로, 주된 성분은 HCA(hydroxyl citric acid)이며, 체지방의 감소에 효과를 발휘한다. 상기 HCA는 탄수화물이 지방으로 전환될 때 사용되는 효소(ATP-citrate lyase)의 활성을 억제하여 체중과 체지방량을 감소시키는 것이다. 상기 가르시니아 캄보지아 추출물은 시판 상품 중 어떠한 것이라도 사용 가능하며, 시판상품은 상기 기능성분인 HCA의 함량은 약 60%로 표준화되어 있다.In the present invention, "Garcinia cambogia extract" is obtained by extracting the peel of Garcinia cambogia fruit with water and drying it. The HCA reduces body weight and fat mass by inhibiting the activity of an enzyme (ATP-citrate lyase) used when carbohydrates are converted into fat. The Garcinia cambogia extract can be used in any commercial product, and the content of HCA, which is the functional component, in the commercial product is standardized to about 60%.

상기 가르시니아 캄보지아 추출물은 본 발명의 식품조성물 내 5~20중량%로 포함할 수 있으며, 구체적으로 5~10중량%를 포함할 수 있다. 가르시니아 캄보지아 추출물의 함량이 5중량% 미만이면 체지방 감소의 효과가 미미하고, 20중량%를 초과할 경우 과량의 가르시니아 캄보지아 추출물이 간독성을 일으킬 우려가 있기 때문이다. 즉, 본 발명은 종래 가르시니아 캄보지아 추출물을 포함하는 건강기능식품에 비해 추출물의 함량을 낮추면서도, 체중감소 및 체지방 분해의 효과를 높일 수 있다는 데 특징이 있다.The Garcinia cambogia extract may be included in 5 to 20% by weight in the food composition of the present invention, specifically 5 to 10% by weight. This is because if the content of the Garcinia cambogia extract is less than 5% by weight, the effect of reducing body fat is insignificant, and if the content exceeds 20% by weight, excessive Garcinia cambogia extract may cause hepatotoxicity. That is, the present invention is characterized in that it can increase the effect of weight loss and body fat decomposition while lowering the content of the extract compared to health functional foods containing the conventional Garcinia cambogia extract.

상기 보리복합발효물은 가수분해효소를 이용하여 보리 및 대두를 가수분해시킨 후 효모 발효를 진행한 것일 수 있다. 구체적으로 프로테아제, 알파-아밀라아제 및 뉴트라제로 이루어진 군에서 선택된 어느 하나 이상의 가수분해효소를 이용하여 가수분해시킨 후 발효를 진행한 것일 수 있다. 보다 더 구체적으로 상기 가수분해효소는 프로테아제, 알파-아밀라아제 및 뉴트라제를 0.5 내지 1 : 1 : 1 내지 1.5의 비율로 혼합한 것일 수 있다.The barley complex fermented product may be obtained by hydrolyzing barley and soybeans using a hydrolase and then yeast fermentation. Specifically, it may be hydrolyzed using at least one hydrolase selected from the group consisting of protease, alpha-amylase, and neutrase, followed by fermentation. More specifically, the hydrolase may be a mixture of protease, alpha-amylase and neutrase at a ratio of 0.5 to 1: 1: 1 to 1.5.

상기 보리복합발효물은 첨가제를 추가할 수 있다. 구체적으로, 상기 보리복합발효물은 산탄검(Xanthan Gum) 및 카르복시메틸 셀루로오즈(carboxymethyl cellulose)로 이루어진 군에서 선택된 하나 이상을 추가로 더 포함할 수 있다. Additives may be added to the barley complex fermentation product. Specifically, the barley complex fermentation product may further include at least one selected from the group consisting of xanthan gum and carboxymethyl cellulose.

상기 “산탄검(Xanthan Gum)”은 식품의 점착성 및 점도를 증가시키고 유화 안정성을 높일 수 있으며, 식품의 물성 및 촉감을 향상시킬 수 있다. 이때 상기 첨가제의 중량비는 발효복합물 100 중량부에 3~7 중량부가 바람직할 것이다. 3중량부 미만이면 첨가 효과가 거의 없고, 7 중량부를 초과하면 첨가제의 향이 진하여 소비자들의 취향에 따라 호불호가 갈릴 수 있다. The "Xanthan Gum" can increase the tackiness and viscosity of food, increase emulsion stability, and improve physical properties and texture of food. At this time, the weight ratio of the additives will be preferably 3 to 7 parts by weight per 100 parts by weight of the fermentation complex. If it is less than 3 parts by weight, there is almost no effect of addition, and if it exceeds 7 parts by weight, the fragrance of the additive is strong, and consumers may like or dislike it depending on their taste.

상기 식품조성물은 율무복합발효물을 추가로 더 포함할 수 있다. The food composition may further include an adlay complex fermentation product.

상기 율무복합발효물은 율무 및 대두를 가수분해효소를 이용하여 가수분해시킨 후 효모 발효를 진행하여 제조한 것일 수 있다. 구체적으로 알파-아밀라아제 및 뉴트라제로 이루어진 군에서 선택된 어느 하나 이상의 가수분해효소를 이용하여 가수분해시킨 후 발효를 진행하여 제조한 것일 수 있다. 보다 더 구체적으로, 상기 가수분해효소는 알파-아밀라아제 및 뉴트라제를 0.5 내지 1 : 1 내지 1.5의 비율로 혼합한 것일 수 있다. The adlay composite fermented product may be prepared by hydrolyzing adlay and soybeans using a hydrolytic enzyme and then proceeding with yeast fermentation. Specifically, it may be prepared by performing fermentation after hydrolysis using any one or more hydrolases selected from the group consisting of alpha-amylase and neutralase. More specifically, the hydrolase may be a mixture of alpha-amylase and neutrase at a ratio of 0.5 to 1: 1 to 1.5.

본 발명에서 대두는 필수 아미노산을 풍부하게 제공하는 양질의 식물성 단백질로써 동물성 단백질과 비교하여 가격이 저렴하므로 동물성 단백의 대체식품으로 사용되고 있다. 또한 대두는 특유의 콩 비린내로 인해 그 이용이 제한받고 있으나 동물성 단백이 가지고 있는 여러 기능적 특성을 나타내고 있으므로 식품산업에 이용되고 있다.In the present invention, soybean is a high-quality vegetable protein that provides abundant essential amino acids and is inexpensive compared to animal protein, so it is used as a substitute for animal protein. In addition, soybeans are limited in their use due to the unique fishy smell of soybeans, but they are used in the food industry because they exhibit various functional characteristics that animal proteins have.

예컨대, 상기 대두는 단백질을 90% 이상 함유하고 있어 식품에 첨가하였을 경우 단백질의 기능적 성질인 수분결합력, 유화성, 기포성 등을 부여할 수 있다. 즉, 식품산업에서 대두는 제품의 물리적 성질을 향상시켜 주고 조직감 개선 및 냉동식품의 결착력 등을 증가시키기 위해 사용될 수 있으며, 동시에 요구르트, 영양음료, 치즈, 아이스크림 등의 가공품에 첨가하여 이용될 수 있다. For example, the soybean contains 90% or more of protein, so when added to food, the functional properties of the protein, such as water binding capacity, emulsification, foamability, etc. can be imparted. That is, in the food industry, soybeans can be used to improve the physical properties of products, improve texture and increase the binding power of frozen foods, and at the same time be added to processed products such as yogurt, nutritional beverages, cheese, and ice cream. .

본 발명에서는 보리, 율무와 같은 곡물에 대두를 함께 혼합하여 발효시킴으로써 효소 활성을 높임과 동시에 탄수화물 분해효과를 높이고자 한다. 또한, 조성물을 분말화한 후 물에 타서 먹을 때에 분산성 및 용해성을 개선하여 소비자들의 선호도를 높이고자 한다. In the present invention, by mixing and fermenting soybeans with grains such as barley and adlay, it is intended to increase enzyme activity and at the same time increase the carbohydrate decomposition effect. In addition, when the composition is powdered and then mixed with water and eaten, dispersibility and solubility are improved to increase consumer preference.

상기 식품조성물은 프로필렌글리콜, 덱스트린, 아라비아검, 피쉬콜라겐, 무수결정포도당, 결정과당 및 고구마엑기스분말을 더 포함할 수 있다.The food composition may further include propylene glycol, dextrin, gum arabic, fish collagen, anhydrous crystalline glucose, crystalline fructose, and sweet potato extract powder.

또한 상기 식품조성물은 전체 조성물에 대하여 가르시니아 캄보지아 추출물 3~7 중량%, 보리복합발효물 10~30 중량%, 율무복합발효물 10~30중량%, 이산화규소 0.5~1.5 중량%, 효소처리스테비아 2~4 중량%, 정제소금 0.5~1.5 중량% 및 스테아린산 마그네슘 1~5 중량%로 포함할 수 있다. 상기 함량을 벗어나는 경우 체중조절용 조제식품에 적합하지 않을 수 있으며, 간 독성 등과 같은 부작용이 발생할 수 있다. In addition, the food composition is 3 to 7% by weight of Garcinia cambogia extract, 10 to 30% by weight of barley complex fermentation, 10 to 30% by weight of barley complex fermentation, silicon dioxide 0.5 to 1.5% by weight, enzyme-treated stevia 2 ~ 4% by weight, 0.5 to 1.5% by weight of refined salt, and 1 to 5% by weight of magnesium stearate. If it is out of the above content, it may not be suitable for weight control formula food, and side effects such as liver toxicity may occur.

본 발명에 따른 상기 체중 조절용 식품조성물은 식품 제조 시에 통상적으로 첨가되는 성분을 포함하며, 예를 들어, 단백질, 탄수화물, 지방, 영양소 및 조미제를 포함할 수 있고, 유효성분 이외에 천연 탄수화물 또는 향미제를 추가 성분으로서 포함할 수 있다. 상기 천연 탄수화물은 모노사카라이드(예컨대, 글루코오스, 프럭토오스 등), 디사카라이드(예컨대, 말토스, 수크로오스 등), 올리고당, 폴리사카라이드(예컨대, 덱스트린, 시클로덱스트린 등), 또는 당알코올(예컨대, 자일리톨, 소르비톨, 에리쓰리톨 등)인 것이 바람직하다. 상기 향미제는 천연 향미제(예컨대, 타우마틴, 스테비아 추출물 등)와 합성 향미제(예컨대, 사카린, 아스파르탐등)를 이용할 수 있다.The food composition for weight control according to the present invention includes ingredients commonly added during food production, and may include, for example, proteins, carbohydrates, fats, nutrients, and seasonings, and natural carbohydrates or flavors in addition to active ingredients. Agents may be included as additional ingredients. The natural carbohydrate is a monosaccharide (eg, glucose, fructose, etc.), a disaccharide (eg, maltose, sucrose, etc.), an oligosaccharide, a polysaccharide (eg, dextrin, cyclodextrin, etc.), or a sugar alcohol (eg, For example, xylitol, sorbitol, erythritol, etc.) is preferable. As the flavoring agent, natural flavoring agents (eg, thaumatin, stevia extract, etc.) and synthetic flavoring agents (eg, saccharin, aspartame, etc.) may be used.

본 발명에 따른 식품조성물은 가르시니아 캄보지아 추출물에 효소 활성이 높은 보리복합발효물 및/또는 율무복합발효물을 첨가하여 체중감량 효과를 높일 수 있으며, 가르시니아 캄보지아의 함량은 낮추어 부작용은 최소화할 수 있다. The food composition according to the present invention can increase the weight loss effect by adding complex fermented barley and/or fermented barley radish complex having high enzyme activity to the Garcinia cambogia extract, and can minimize side effects by lowering the content of Garcinia cambogia.

또한 체중조절용 식품조성물은 물에 용해성이 우수하여, 섭취가 용이하므로 사람들이 쉽게 음용할 수 있다. In addition, the food composition for weight control is excellent in solubility in water, so people can easily drink it because it is easy to ingest.

본 발명의 효과는 상기 효과로 한정되는 것은 아니며, 본 발명의 상세한 설명 또는 청구범위에 기재된 발명의 구성으로부터 추론 가능한 모든 효과를 포함하는 것으로 이해되어야 한다.The effects of the present invention are not limited to the above effects, and should be understood to include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

도 1은 본 발명에 따른 체중조절용 식품 조성물의 탄수화물 분해율의 결과를 나타낸 것이다.
도 2는 본 발명에 따른 체중조절용 식품 조성물의 체중감량 효과(몸무게, 허리 둘레 변화)를 측정한 결과를 나타낸 것이다.
도 3은 본 발명에 따른 체중조절용 식품 조성물의 체중감량 효과(체지방율, BMI)를 측정한 결과를 나타낸 것이다.
Figure 1 shows the results of the carbohydrate decomposition rate of the food composition for weight control according to the present invention.
Figure 2 shows the results of measuring the weight loss effect (change in body weight, waist circumference) of the food composition for weight control according to the present invention.
Figure 3 shows the results of measuring the weight loss effect (body fat percentage, BMI) of the food composition for weight control according to the present invention.

이하, 첨부된 도면을 참고하여 본 발명의 실시예에 관하여 상세히 서술하나, 하기 실시예에 의해 본 발명이 제한되지 아니함은 자명하다.Hereinafter, the embodiments of the present invention will be described in detail with reference to the accompanying drawings, but it is obvious that the present invention is not limited by the following examples.

제조예 1. 대두분말의 제조Preparation Example 1. Preparation of soybean powder

대두를 10~20시간 동안 불린 후 탈피하여 50~60메쉬(mesh) 크기의 분말 형태로 분쇄한다. 분쇄한 콩 100 중량부에 글루텐 45~60 중량부를 혼합한 혼합재료 100 중량부에 정제수 70~80 중량부를 투입하여 반죽한 반죽물을 호모믹서에서 교반한다. 이때 정제수의 온도는 60~ 70℃이고, 교반시간은 5~ 10분이며, 상기 호모믹서의 회전속도는 2,500~ 3,500 rpm의 조건에서 진행한다. After soaking the soybeans for 10 to 20 hours, they are peeled and ground into powder with a size of 50 to 60 mesh. 70 to 80 parts by weight of purified water is added to 100 parts by weight of a mixed material in which 45 to 60 parts by weight of gluten is mixed with 100 parts by weight of pulverized soybeans, and the kneaded dough is stirred in a homomixer. At this time, the temperature of the purified water is 60 to 70 ° C, the stirring time is 5 to 10 minutes, and the rotation speed of the homomixer is 2,500 to 3,500 rpm.

제조예 2. 보리 발효물의 제조Preparation Example 2. Preparation of fermented barley

2-1. 보리 발효물의 제조 단계2-1. Production steps of fermented barley

보리원물을 준비하고 이를 마쇄한다. 이때 햄머밀을 이용하여 875rpm 및 337kg/40min 조건 하 마쇄를 수행할 수 있다. 마쇄된 보리에 정제수를 첨가하여 10 내지 14 시간 동안 침지하였다. 이때 마쇄 보리 : 정제수는 1 : 3~6의 중량비로 사용한다. 침지가 완료된 후 보리를 90 ℃에서 1 내지 3시간 증자하여 호화를 수행하였다. 호화된 보리에 알파 아밀라아제(α-amylase)를 첨가하여 70 내지 90 ℃에서 1 내지 3 시간 처리하여 1차 효소 가수분해를 수행하였다.Prepare raw barley and grind it. At this time, grinding may be performed using a hammer mill under conditions of 875 rpm and 337 kg/40 min. Purified water was added to ground barley and soaked for 10 to 14 hours. At this time, ground barley: purified water is used in a weight ratio of 1: 3 to 6. After the immersion was completed, gelatinization was performed by steaming barley at 90 ° C. for 1 to 3 hours. Alpha amylase (α-amylase) was added to gelatinized barley and treated at 70 to 90 ° C. for 1 to 3 hours to perform primary enzymatic hydrolysis.

그 후, 1차 효소 가수분해물에 프로테아제(protease), 알파-아밀라아제 및 뉴트라제(neutrase)의 혼합물을 첨가하여 50 내지 70 ℃에서 1 내지 3시간 처리하여 2차 효소 가수분해를 수행하였다. 그리고 2차 효소 가수분해물에 당화효소인 베타-글루카나제(glucanase)를 첨가하여 50 내지 70 ℃에서 12 내지 36시간 반응시켜 3차 효소 가수분해를 수행하여 당화액을 제조하였다.Thereafter, a mixture of protease, alpha-amylase, and neutrase was added to the first enzyme hydrolyzate and treated at 50 to 70 ° C. for 1 to 3 hours to perform second enzyme hydrolysis. In addition, beta-glucanase, a saccharification enzyme, was added to the secondary enzymatic hydrolyzate and reacted at 50 to 70 ° C. for 12 to 36 hours to perform tertiary enzymatic hydrolysis to prepare a saccharification solution.

상기 당화액에 효모(Saccharomyces cerevisiae)를 첨가하여 20 내지 40℃에서 12 내지 36 시간 동안 효모 발효를 진행하며. 그 후 효모 발효물에 유산균인 바실러스 서브틸리스(Bacillus subtilis) 및 바이셀라 시바리아(Weissella cibaria)를 첨가하여 20 내지 40 ℃에서 12 내지 36 시간 동안 유산균 발효를 진행하여, 보리 발효물을 제조하였다. 상기 보리 발효물을 5,000 내지 8,000 rpm에서 20 내지 40분간 원심분리하여 상층액을 분리하고 가압 살균하였다.Yeast ( Saccharomyces cerevisiae ) is added to the saccharification solution and yeast fermentation is performed at 20 to 40 ° C. for 12 to 36 hours. Thereafter, lactic acid bacteria, Bacillus subtilis and Weissella cibaria , were added to the yeast fermentation product, and lactic acid bacteria fermentation was performed at 20 to 40 ° C. for 12 to 36 hours to prepare fermented barley. . The fermented barley was centrifuged at 5,000 to 8,000 rpm for 20 to 40 minutes to separate the supernatant and sterilized under pressure.

2-2. 첨가제를 포함하는 보리 발효물의 제조 단계2-2. Preparation step of fermented barley containing additives

<제조예 2-1>의 보리 발효물을 제조한 후, 상기 발효물에 산탄검(Xanthan Gum) 및 카르복시메틸 셀루로오즈(carboxymethyl cellulose)를 보리 발효물 100 중량부에 3~7 중량부 비율로 첨가하였다. After preparing the fermented barley product of <Preparation Example 2-1>, 3 to 7 parts by weight of xanthan gum and carboxymethyl cellulose were added to 100 parts by weight of the fermented barley product. was added as

제조예 3. 율무 발효물의 제조Production Example 3. Preparation of fermented adlay

율무원물을 준비하고, 이를 마쇄한다. 이때 햄머밀을 이용하여 875rpm 및 337kg/40min 조건 하 마쇄를 수행할 수 있다. 마쇄된 율무에 정제수를 첨가하여 8 내지 10시간 동안 침지하였다. 이때 마쇄 율무 : 정제수는 1 : 3~6의 중량비로 사용한다. 침지가 완료된 후 알파-아밀라아제 및 뉴트라제의 혼합물을 첨가하여 50 내지 70 ℃에서 1 내지 3시간 처리하여 1차 효소 가수분해를 수행하였다. 그리고 1차 효소 가수분해물에 당화효소인 베타-글루카나제(glucanase)를 첨가하여 50 내지 70 ℃에서 12 내지 36시간 반응시켜 2차 효소 가수분해를 수행하여 당화액을 제조하였다.Prepare adlay raw water and grind it. At this time, grinding may be performed using a hammer mill under conditions of 875 rpm and 337 kg/40 min. Purified water was added to the ground adlay and soaked for 8 to 10 hours. At this time, ground adlay: purified water is used in a weight ratio of 1: 3 to 6. After the immersion was completed, a mixture of alpha-amylase and neutrase was added and treated at 50 to 70 °C for 1 to 3 hours to perform primary enzymatic hydrolysis. In addition, beta-glucanase, a saccharification enzyme, was added to the first enzymatic hydrolyzate and reacted at 50 to 70 ° C. for 12 to 36 hours to perform secondary enzymatic hydrolysis to prepare a saccharification solution.

상기 당화액에 효모(Saccharomyces cerevisiae)를 첨가하여 20 내지 40℃에서 12 내지 36 시간 동안 효모 발효를 진행하며. 그 후 효모 발효물에 유산균인 바실러스 서브틸리스(Bacillus subtilis), 아스퍼질러스 오리재(Aspergillus oryzae)를 첨가하여 20 내지 40 ℃에서 12 내지 36 시간 동안 유산균 발효를 진행하여, 율무 발효물을 제조하였다. 상기 율무 발효물을 5,000 내지 8,000 rpm에서 20 내지 40분간 원심분리하여 상층액을 분리하고 가압 살균하였다.Yeast ( Saccharomyces cerevisiae ) is added to the saccharification solution and yeast fermentation is performed at 20 to 40 ° C. for 12 to 36 hours. Thereafter, lactic acid bacteria Bacillus subtilis and Aspergillus duck material were added to the yeast fermented product, and lactic acid bacteria fermentation was performed at 20 to 40 ° C. for 12 to 36 hours to prepare a fermented adlay. did The fermented adlay was centrifuged at 5,000 to 8,000 rpm for 20 to 40 minutes to separate the supernatant and sterilized under pressure.

제조예 4. 보리복합발효물 제조Preparation Example 4. Barley Complex Fermentation

4-1. 보리복합발효물의 제조 단계4-1. Manufacturing step of barley complex fermented product

대두분말 및 보리를 1 : 1의 중량비로 혼합한 후, 스팀으로 증자 후 냉각하여 가수분해효소를 기질대비 1% 분사하고 45℃에서 4시간 동안 처리하였다. 이때, 사용한 효소는 단백질 분해 효소인 프로테아제, 알파-아밀라아제 및 뉴트라제를 1 : 1 : 1의 중량비로 혼합한 것이다. 이후 80℃에서 30분간 효소 실활 처리 후, 15℃의 온도가 되도록 냉각하고, 바실러스 서브틸리스 및 바이셀라 시바리아를 접종하여 72시간 동안 30~ 35℃, 습도 95% 조건으로 배양, 발효하였다. Soybean powder and barley were mixed at a weight ratio of 1: 1, steamed, cooled, sprayed with 1% hydrolase compared to the substrate, and treated at 45 ° C. for 4 hours. At this time, the enzyme used is a mixture of protease, alpha-amylase and neutrase in a weight ratio of 1: 1: 1. Thereafter, after enzyme inactivation treatment at 80 ° C. for 30 minutes, cooling to a temperature of 15 ° C., inoculation of Bacillus subtilis and Visella cibaria, culture and fermentation at 30-35 ° C. and 95% humidity for 72 hours.

4-2. 첨가제를 포함하는 보리복합발효물의 제조 단계4-2. Manufacturing step of barley complex fermented product containing additives

<제조예 4-1>의 보리복합발효 효소를 제조한 후, 배양 후, 배양물에 첨가제인 산탄검 및 카르복시메틸 셀루로오즈를 5중량부 첨가하여 출국 및 건조(24시간, 50℃, 수분 8% 이하)하여 건조물을 만들고, 건조물(배양물)을 40~ 60메쉬(mesh)로 분쇄하여 분말 형태의 발효 조성물(즉, 성형물)을 수득하였다.After preparing the barley complex fermentation enzyme of <Preparation Example 4-1>, culturing, adding 5 parts by weight of xanthan gum and carboxymethyl cellulose as additives to the culture, leaving the country and drying (24 hours, 50 ℃, moisture) 8% or less) to make a dried product, and pulverize the dried product (culture) to 40 to 60 mesh to obtain a powdered fermentation composition (ie, molded product).

제조예 5. 율무복합발효물의 제조Preparation Example 5. Production of adlay complex fermented product

대두분말 및 율무를 1 : 1의 중량비로 혼합한 후, 스팀으로 증자 후 냉각하여 가수분해효소를 기질대비 1% 분사하고 45℃에서 4시간 동안 처리하였다. 이때, 사용한 효소는 단백질 분해 효소인 알파-아밀라아제 및 뉴트라제를 1 : 1의 중량비로 혼합한 것이다. 이후 80℃에서 30분간 효소 실활 처리 후, 15℃의 온도가 되도록 냉각하고, 바실러스 서브틸리스, 아스퍼질러스 오리재를 접종하여 72시간 동안 30~ 35℃, 습도 95% 조건으로 배양, 발효하였다. 배양 후, 건조(24시간, 50℃, 수분 8% 이하)하여 건조물을 만들고, 건조물(배양물)을 40~ 60메쉬(mesh)로 분쇄하여 분말 형태의 발효 조성물(즉, 성형물)을 수득하였다.Soybean powder and adlay were mixed in a weight ratio of 1: 1, then steamed, cooled, sprayed with 1% hydrolase compared to the substrate, and treated at 45 ° C. for 4 hours. At this time, the enzyme used is a mixture of alpha-amylase and neutrase, which are proteolytic enzymes, in a weight ratio of 1:1. Then, after enzyme inactivation treatment at 80 ° C for 30 minutes, cooling to a temperature of 15 ° C, inoculation of Bacillus subtilis and Aspergillus duck material, incubation and fermentation at 30 ~ 35 ° C and 95% humidity for 72 hours. . After culturing, drying (24 hours, 50 ℃, less than 8% moisture) to make a dried product, and the dried product (culture) was pulverized with 40 ~ 60 mesh (mesh) to obtain a powdered fermentation composition (ie, molded product) .

실시예 및 비교예. 가르시니아 캄보지아 추출물 및 발효복합물을 포함하는 체중조절용 식품 조성물Examples and Comparative Examples. Food Composition for Weight Control Containing Garcinia Cambogia Extract and Fermentation Complex

제조예 1 내지 5에서 제조한 복합발효 효소, 가르시니아 캄보지아 추출물, 이산화규소, 효소처리스테비아, 정제소금, 스테아린산 마그네슘, 프로필렌글리콜, 덱스트린, 아라비아검, 피쉬콜라겐, 대두, 무수결정포도당, 결정과당 및 고구마엑기스분말을 혼합하여 체중조절용 분말형 식품 조성물을 제조하였다. Complex fermentation enzyme prepared in Preparation Examples 1 to 5, Garcinia cambogia extract, silicon dioxide, enzyme-treated stevia, refined salt, magnesium stearate, propylene glycol, dextrin, gum arabic, fish collagen, soybean, anhydrous crystalline glucose, fructose and sweet potato A powdered food composition for weight control was prepared by mixing the extract powder.

원료(중량%)Raw material (% by weight) 실시예 1Example 1 실시예 2Example 2 비교예 1Comparative Example 1 비교예 2Comparative Example 2 보리복합발효물barley complex fermented product 3030 -- -- -- 첨가제 포함 보리복합발효물Complex fermented barley with additives -- 1515 -- -- 율무복합발효물Adlay complex fermented product -- 1515 -- 3030 보리 발효물fermented barley -- -- 1515 -- 율무발효물Adlay fermented product -- -- 1515 -- 가르시니아 캄보지아 추출분말Garcinia Cambogia Extract Powder 55 55 55 55 이산화규소silicon dioxide 1One 1One 1One 1One 효소처리스테비아Enzymatically Treated Stevia 33 33 33 33 정제소금refined salt 1One 1One 1One 1One 스테아린산 마그네슘magnesium stearate 33 33 33 33 프로필렌글리콜propylene glycol 1One 1One 1One 1One 덱스트린dextrin 77 77 77 77 아라비아검gum arabic 88 88 88 88 피쉬콜라겐fish collagen 33 33 33 33 대두Big head 88 88 88 88 무수결정포도당anhydrous crystalline glucose 55 55 55 55 결정과당fructose 1515 1515 1515 1515 고구마엑기스분말Sweet Potato Extract Powder 1010 1010 1010 1010 합계Sum 100100 100100 100100 100100

실험예 1. 탄수화물 분해 효과 본 발명의 가르시니아 캄보지아 추출물 및 발효복합물을 포함하는 식품 조성물이 탄수화물 분해에 미치는 효과를 알아보기 위해 실시예 1, 2 또는 비교예 1, 2를 이용하여 실험을 수행하였다. 전분과 아밀라아제를 혼합한 용액(대조군), 전분 및 실시예 1, 2 및 비교예 1, 2의 조성물 섞은 혼합물을 각각 시험관에 넣고, 글루코오스(glucose) 표준 용액, 증류수(DW), DNS(3,5-dinitrosalicylicacid) 시약을 차례로 취하고 잘 섞었다. 항온 수조에서 15분 동안 반응하게 한 다음 20℃ 이하의 수조 또는 얼음(ICE)에서 15 분 동안 냉각하여 반응을 멈추었다. 발색된 반응액에 증류수를 넣어 희석한 후 분광광도계 546 nm에서 흡광도를 측정하였다. 이 수치를 그래프로 도식하여 표준곡선을 작성하고, 글루코오스 표준 용액과 같은 방법으로 반응시킨 시료의 흡광도를 측정하였다. Experimental Example 1. Carbohydrate decomposition effect Experiments were conducted using Examples 1 and 2 or Comparative Examples 1 and 2 to examine the effect of the food composition containing the Garcinia cambogia extract and the fermented complex of the present invention on carbohydrate decomposition. A solution of starch and amylase (control), starch and the mixture of the compositions of Examples 1 and 2 and Comparative Examples 1 and 2 were put into a test tube, respectively, and glucose standard solution, distilled water (DW), DNS (3, 5-dinitrosalicylicacid) reagents were taken sequentially and mixed well. After reacting in a constant temperature water bath for 15 minutes, the reaction was stopped by cooling in a water bath below 20° C. or ice (ICE) for 15 minutes. After diluting the colored reaction solution with distilled water, the absorbance was measured at 546 nm with a spectrophotometer. A standard curve was prepared by plotting these values in a graph, and the absorbance of the reacted sample was measured in the same way as the glucose standard solution.

상기 실험 결과, 도 1에 나타난 바와 같이 대조군인 아밀라아제보다 실시예 1의 조성물이 들어간 식품의 탄수화물 분해율이 92.4% 증가하며, 실시예 2의 조성물은 95.9%로 증가함을 알 수 있었다. 이는 본 발명에 따른 발효물에 함유되어 있는 탄수화물 분해 효소가 아밀라아제보다 분해 효과가 더 뛰어남을 보여준다.As a result of the experiment, as shown in FIG. 1, it was found that the carbohydrate decomposition rate of the food containing the composition of Example 1 increased by 92.4%, and the composition of Example 2 increased by 95.9%, compared to the control amylase. This shows that the carbohydrate decomposition enzyme contained in the fermented product according to the present invention has a more excellent decomposition effect than amylase.

실험예 2. 체중감량 효과Experimental Example 2. Weight loss effect

본 발명의 가르시니아 캄보지아 추출물 및 발효복합물을 포함하는 식품 조성물이 체중감량에 미치는 효과를 알아보기 위하여 실시예 1 및 2를 이용하여 실험을 수행하였다. 평균 체중 65 내지 70 kg의 30대 여성 5명과 평균 체중 75 ~ 85kg의 40대 남성 5 명을 대상으로 각각 45 일 동안 실시예 1 및 2의 식품조성물로 구성된 분말을 생수에 타서 하루에 1회 식사하기 전에 먹게하였다. 이후 섭취하기 전과 후의 체중, 복부, 체지방율, BMI(body mass index)를 측정하였다. Experiments were conducted using Examples 1 and 2 to investigate the effect of the food composition containing the Garcinia cambogia extract and the fermented complex of the present invention on weight loss. 5 women in their 30s with an average weight of 65 to 70 kg and 5 men in their 40s with an average weight of 75 to 85 kg were given a meal once a day by dissolving the powder composed of the food composition of Examples 1 and 2 in bottled water for 45 days, respectively. ate before doing it. Then, before and after intake, body weight, abdomen, body fat percentage, and body mass index (BMI) were measured.

그 결과, 도 2에 나타난 바와 같이 실시예 1의 조성물을 포함하는 분말을 45일동안 섭취한 경우 체중이 섭취 전 남자 평균 79.8 kg에서 74.5kg로 6.6% 체중이 감소하였다. 또한 실시예 2의 조성물을 포함하는 분말을 45일동안 섭취시킨 결과 체중이 섭취 전 남성 평균 81.6kg에서 75.6kg으로 7.4% 감소하였다. 여성의 경우 실시예 1의 조성물을 포함하는 분말을 섭취하였을 때 평균 68.8kg에서 64.8kg으로 5.8% 체중이 감소하였으며, 실시예 2의 조성물을 포함하는 분말을 섭취 시에는 68.5kg에서 64.2kg으로 6.3% 감소하였다. 또한 분말 섭취 전과 후의 허리 및 복부 둘레를 7일 간격으로 측정한 결과, 남자는 평균 4.5~4.7 cm, 여자는 평균 3.8~4.1 cm 정도 허리 둘레 사이즈가 감소한 것으로 나타났다. As a result, as shown in FIG. 2, when the powder containing the composition of Example 1 was ingested for 45 days, the average male body weight before ingestion was 79.8 kg to 74.5 kg, a decrease of 6.6%. In addition, as a result of ingesting the powder containing the composition of Example 2 for 45 days, the average weight of men before ingestion was 81.6 kg to 75.6 kg, a decrease of 7.4%. In the case of women, when ingesting the powder containing the composition of Example 1, the average body weight decreased by 5.8% from 68.8 kg to 64.8 kg, and when ingesting the powder containing the composition of Example 2, the weight decreased from 68.5 kg to 64.2 kg by 6.3 kg. % decreased. In addition, as a result of measuring the waist and abdominal circumferences before and after powder intake at 7-day intervals, men's waist circumference decreased by an average of 4.5 to 4.7 cm and women by an average of 3.8 to 4.1 cm.

마지막으로, 도 3에 나타난 바와 같이 체지방율 및 BMI를 각각 측정한 결과, 남자의 체지방율은 19.2~19.8%로 감소하였으며, 여자는 21.1~22.8%로 감소하였다. BMI 지수의 경우 남자는 0.61~0.65, 여자는0.45~0.46로 감소하였다. 이와 같은 결과는 본 발명에 따른 식품 조성물이 체중, 허리 둘레 및 체지방을 감소시키는 효과가 있음을 보여준다.Finally, as shown in Figure 3, as a result of measuring body fat percentage and BMI, respectively, the body fat percentage of men decreased to 19.2 ~ 19.8%, and women decreased to 21.1 ~ 22.8%. In the case of the BMI index, men decreased to 0.61~0.65 and women decreased to 0.45~0.46. These results show that the food composition according to the present invention has an effect of reducing body weight, waist circumference and body fat.

실험예 3. 관능 검사Experimental Example 3. Sensory test

상기 실시예에 의하여 제조된 체중조절용 식품에 대하여 관능 검사를 실시하였다. 관능검사는 20~40대 성인남녀 각각 10명의 평가단이 실시예로 제조한 조성물을 투입한 식품의 냄새, 맛, 향을 평가하였다. 각 평가항목을 5점 표시법으로 채택하였고, 각 항목에 대한 평균점수를 계산하였다.A sensory test was performed on the food for weight control prepared according to the above example. In the sensory test, 10 adult males and females in their 20s and 40s each evaluated the smell, taste, and aroma of the food into which the composition prepared in the example was added. Each evaluation item was adopted as a 5-point notation, and the average score for each item was calculated.

실시예 1Example 1 실시예 2Example 2 냄새smell 3.33.3 3.33.3 taste 3.53.5 3.63.6 incense 3.13.1 3.13.1

그 결과, 상기 [표 2]에 나타난 바와 같이 실시예의 체중조절용 조성물을 포함하는 식품의 냄새, 맛 및 향에서 보통 이상의 평점을 받으며, 이를 섭취하는 소비자들이 전반적으로 선호할 수 있는 것으로 나타났다. 전술한 본 발명의 설명은 예시를 위한 것이며, 본 발명이 속하는 기술분야의 통상의 지식을 가진 자는 본 발명의 기술적 사상이나 필수적인 특징을 변경하지 않고서 다른 구체적인 형태로 쉽게 변형이 가능하다는 것을 이해할 수 있을 것이다. 그러므로 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며 한정적이 아닌 것으로 이해해야만 한다. 예를 들어, 단일형으로 설명되어 있는 각 구성 요소는 분산되어 실시될 수도 있으며, 마찬가지로 분산된 것으로 설명되어 있는 구성 요소들도 결합된 형태로 실시될 수 있다.본 발명의 범위는 후술하는 청구범위에 의하여 나타내어지며, 청구범위의 의미 및 범위 그리고 그 균등 개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다.As a result, as shown in [Table 2], the odor, taste and aroma of the food containing the composition for weight control of the examples received above average ratings, and it was found that consumers who consumed it could prefer it overall. The above description of the present invention is for illustrative purposes, and those skilled in the art can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, the embodiments described above should be understood as illustrative in all respects and not limiting. For example, each component described as a single type may be implemented in a distributed manner, and likewise components described as distributed may be implemented in a combined form. The scope of the present invention is set forth in the following claims. It should be construed that all changes or modified forms derived from the meaning and scope of the claims and equivalent concepts are included in the scope of the present invention.

Claims (1)

가르시니아 캄보지아 추출물, 보리복합발효물, 율무복합발효물, 이산화규소, 효소처리스테비아, 정제소금 및 스테아린산 마그네슘을 포함하는 체중조절용 식품조성물에 있어서,
상기 보리복합발효물은 프로테아제, 알파-아밀라아제 및 뉴트라제를 1 : 1 : 1의 비율로 혼합한 가수분해효소를 이용하여 보리 및 대두가 1:1의 중량비로 혼합된 것을 가수분해시킨 후 바실러스 서브틸리스(Bacillus subtilis) 및 바이셀라 시바리아(Weissella cibaria)를 접종하여 발효시킨 뒤, 상기 보리복합발효물 100 중량부 대비 산탄검(Xanthan Gum) 및 카르복시메틸 셀루로오즈(carboxymethyl cellulose)를 5 중량부 첨가한 것이고,
상기 율무복합발효물은 알파-아밀라아제 및 뉴트라제를 1 : 1의 비율로 혼합한 가수분해효소를 이용하여 율무 및 대두가 1:1의 중량비로 혼합된 것을 가수분해시킨 후 바실러스 서브틸리스(Bacillus subtilis), 아스퍼질러스 오리재(Aspergillus oryzae)를 접종하여 발효시킨 것이며,
상기 식품조성물은 전체 조성물에 대하여 가르시니아 캄보지아 추출물 3~7 중량%, 보리복합발효물 10~30 중량%, 율무복합발효물 10~30중량%, 이산화규소 0.5~1.5 중량%, 효소처리스테비아 2~4 중량%, 정제소금 0.5~1.5 중량% 및 스테아린산 마그네슘 1~5 중량%를 포함하는 것인, 체중조절용 식품조성물.
In the food composition for weight control comprising Garcinia cambogia extract, barley complex fermentation product, barley complex fermentation product, silicon dioxide, enzyme-treated stevia, refined salt and magnesium stearate,
The barley complex fermented product is obtained by hydrolyzing a mixture of barley and soybeans in a weight ratio of 1: 1 using a hydrolase in which protease, alpha-amylase and neutrase are mixed in a ratio of 1: 1: 1, and then Bacillus sub After inoculating and fermenting Bacillus subtilis and Weissella cibaria , 5 parts by weight of xanthan gum and carboxymethyl cellulose were added to 100 parts by weight of the barley complex fermented product. is added,
The adlay complex fermented product is obtained by hydrolyzing a mixture of adlay and soybeans in a weight ratio of 1: 1 using a hydrolase in which alpha-amylase and neutrase are mixed in a ratio of 1: 1, and then Bacillus subtilis ( Bacillus subtilis ), Aspergillus duck material ( Aspergillus oryzae ) inoculated and fermented,
The food composition is 3 to 7% by weight of Garcinia cambogia extract, 10 to 30% by weight of barley complex fermentation, 10 to 30% by weight of barley complex fermentation, 0.5 to 1.5% by weight of silicon dioxide, 2 to 10% by weight of enzyme-treated stevia, relative to the total composition 4% by weight, 0.5 to 1.5% by weight of refined salt and 1 to 5% by weight of magnesium stearate, a food composition for weight control.
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