KR102476932B1 - Extraction method of hinoki cypress - Google Patents

Abstract

The present invention relates to a cypress extraction method, and more specifically, i) cutting cypress leaves and branches into a range of 1 to 5 cm after washing; ii) It relates to a cypress extraction method comprising mixing 300 to 2,000 parts by weight of polyol with respect to 100 parts by weight of cut cypress leaves and branches and extracting at a temperature in the range of 10 to 50 ° C for 4 to 30 hours. In extracting the active ingredient of cypress, which is known to have antibacterial and antiseptic effects, the extract obtained by extracting only the active ingredient was solved by solving the problem of the existing extraction method, which was impossible to use as a cosmetic raw material due to the problem of by-products with strong fragrance and color. Provides a way to obtain it.

Description

Cypress extraction method {EXTRACTION METHOD OF HINOKI CYPRESS}

The present invention relates to a cypress extraction method, and more particularly, i) cutting cypress leaves and branches into a range of 1 to 5 cm after washing; ii) A cypress extraction method including the step of mixing 300 to 2,000 parts by weight of polyol with respect to 100 parts by weight of cut cypress leaves and branches and extracting at a temperature in the range of 10 to 50 ° C. for 4 to 30 hours.

Cypress trees emit a substance called phytoncide, which is an aromatic substance that trees emit to protect themselves from various pests and bacteria. The main component of phytoncide is an organic compound of the terpene family, It is known to have excellent effects in removing harmful substances in the air, antibacterial effect, immune function boosting effect, as well as preventing atopy and allergy. Therefore, there are increasing cases of extracting antibacterial and antiseptic ingredients from leaves and branches of cypress trees and applying them to various household items such as cosmetic raw materials.

In the case of the existing cypress extraction method, it is generally manufactured in three ways. First, in the case of European and some domestic companies, after removing moisture by drying leaves, roots, branches, etc. of plants, etc., extracting at room temperature or heating with a solvent such as ethanol, removing the solvent in a heated and vacuum state, concentrating There is a method of obtaining a powdery extract by freeze-drying.

Second, there is a method of obtaining essential oil and extraction water, which are generally called essential oils, by brewing active ingredients of plants together with steam water through a pressurized steam extraction method. Korean Patent Registration No. 10-1514524 discloses a) drying cypress leaves in a low-temperature dryer, then cutting the dried cypress leaves into particles of 1 to 3 mm and powdering them to pass through 1,000 to 2,000 mesh; b) supplying distilled water by putting the cypress leaves of step a) in a trapping net and putting them in a distillation tank; c) Heating the distillation tank to supply steam so that it passes through the leaves of the cypress tree, and moving the water vapor containing the essential oil extracted from the steam to a cooler to separate it into cypress oil and cypress water Antibiotics, characterized in that produced An antibacterial detergent composition having a growth inhibitory function of resistant strains is disclosed.

Third, there is an extraction method of essential oil using a supercritical extraction method. Korean Patent Registration No. 10-1534837 discloses a drying step of drying for 24 hours by applying cold air at 35 ° C. to the leaves of cypress washed with water to remove foreign substances; An extraction step of pulverizing the dried cypress leaves with a blender and then extracting a cypress supercritical extract using a supercritical fluid extraction method; Including, a formulation step of preparing the cypress supercritical extract as body care; in the extraction step, the cypress supercritical extract is extracted for 180 minutes by adding supercritical carbon dioxide at a temperature of 40 ° C and a pressure of 150 bar to the dried cypress leaves In the formulation step, the body care contains 1.0% by weight of cetearyl alcohol, 1.6% by weight of glyceryl stearate, 0.5% by weight of beeswax, 0.5% by weight of hydrogenated lecithin, and 3.0% by weight of neopentyl glycol dicaprate. Hydrogenated polydecene 4.0% by weight, capric triglyceride 2.0% by weight, cyclopentasiloxane 5.0% by weight, tocopheryl acetate 0.2% by weight, dimethicone 0.3% by weight, propylparaben 0.1% by weight, trisodium EDTA 0.02 wt%, methylparaben 0.2 wt%, glycerin 6.5 wt%, dipropylene glycol 4.5 wt%, xanthan gum 0.03 wt%, carbomer 0.16 wt%, potassium hydroxide 0.071 wt%, phenoxyethanol 0.2 wt% A method for producing a body care cosmetic composition using a cypress supercritical extract, characterized in that it has moisturizing activity by being composed of 0.1% by weight of dipotassium glycyrrhizate, 3.5% by weight of the cypress supercritical extract, and the remaining amount of purified water is disclosed have.

In addition, in the case of most domestic extract manufacturers, 1,3-butylene glycol or 1,3-propanediol, purified water, etc. are used to boil plants at a level of 60 ~ 80 ℃ to extract active substances, and The liquid phase is diluted with purified water and used as a raw material for cosmetics.

However, the aforementioned cypress extraction method has limitations in its use as a raw material for cosmetics. In the case of the first ethanol extraction method, it is possible to produce effective substances such as polyphenols, which are unique to plants, in a relatively high ratio, but the extraction of pigments such as chlorophyll is also increased at the same time, so that the color is brown, Most of them come out very dark in the form of dark brown or blue green. Therefore, such a powdery extract is used in a line where a special concept or cosmetic composition is at least tan to dark brown, or a very small amount is added to the advantage of this extraction method, and the color of the cosmetic is colorless or white or the color of the cosmetic is not violated It can be seen that there is a limit. In this case, it is safe to say that there is no unique function of the extract, and it is mostly used for the purpose of the concept.

The second pressurized steam extraction method has a very pale yellow system or is close to colorless in terms of color, but is limited to use in cosmetic compositions because the nature itself is oil or essential oil (generally essential oil), and in addition, the plant's own fragrance It comes out thickly and it becomes difficult to steer the cosmetic composition. In addition, the extraction water, which is a kind of by-product from the process - the water from which some of the water-soluble active ingredients are dissolved through steam extraction - has a plant-specific fragrance like oil or essential oil (essential oil), so it has a strong fragrance, but the plant itself retains it. The amount of the active ingredient is extremely weak, and in particular, the antibacterial and antiseptic effects, which are the main focus of the present invention, were obtained as a result of the test. Accordingly, the extracted water produced by the pressurized steam extraction method is used after a separate preservative treatment because corruption occurs in the distribution process. Typically, 1,2-hexanediol, which is a comparative object of the present invention, is used as a preservative in cosmetic compositions at a level of 2 to 3%.

Third, the supercritical extraction method has the disadvantage of excessive equipment and process costs, and at the same time, the same disadvantage as the pressurized steam extraction method exists.

1,3-butylene glycol and 1,3-propanediol, or the method of boiling and brewing using purified water, which is used by most domestic extract companies, has a color similar to the first ethanol extraction method, and its use is limited. The content used in the actual cosmetic composition is less than several percent, and depending on the temperature, time, and content of heating, the color becomes darker or the plant's unique fragrance is strong, so most of them are boiled and extracted and then diluted in purified water. Extracts prepared in such a way have a problem of rather decaying in the distribution process, so 1,2-hexanediol, which is a comparative target of the present invention, is mostly used as a preservative. Existing patents and papers used ethanol extraction, steam extraction, and supercritical extraction to evaluate only the effect of cypress. However, as described above, these methods fundamentally cause problems in color and steering of the cosmetic composition, and also require separate preparation (emulsifier, solubilizer, solvent, etc.) treatment to stably mix in the cosmetic composition, so the effect of cypress Except for confirmation, there is a clear crowd for commercial use.

Therefore, the present invention is a part of the natural extract at a level that does not affect the color and flavoring of the cosmetic composition regardless of the composition ratio of the cosmetic composition, and thermal damage or physical Without damage, it was possible to satisfy the effective extraction of substances with antibacterial and antiseptic effects of the cypress itself at the same time.

Republic of Korea Patent No. 10-1514524 Republic of Korea Patent No. 10-1534837

Therefore, the technical problem to be achieved by the present invention is to extract the active ingredient of cypress, which is known to have antibacterial and antiseptic effects. It is to provide a cypress extraction method that solves the problem.

In order to achieve the above technical problem, the present invention includes: i) cutting the cypress leaves and branches into a range of 1 to 5 cm after washing; ii) mixing 300 to 2,000 parts by weight of polyol with respect to 100 parts by weight of cut cypress leaves and branches and extracting at a temperature in the range of 10 to 50 ° C. for 4 to 30 hours.

In addition, the present invention provides a cypress extraction method further comprising the process of aging for 10 to 30 hours at room temperature after iii) extraction.

In the present invention, the polyol is 1,3-propanediol, 1,3-butylene glycol, 2-methyl-1,3-propanediol, 1,2-butylene glycol, 2,3-butylene glycol, Dipropylene glycol, 1,5-pentanediol, 1,6-hexanediol, 1,8-octanediol, 1,2-decanediol, 1,10-decanediol, butylethylpropanediol, campanediol and It provides a cypress extraction method, characterized in that at least one selected from the group consisting of polyethylene glycol.

In addition, the present invention provides a cypress extract extracted by the above method.

The present invention extracts the active ingredient of cypress known to have antibacterial and antiseptic effects, and extracts only the active ingredient by solving the problem of the existing extraction method, which could not be used as a cosmetic raw material due to the problem of by-products with strong fragrance and color. A method by which an extract can be obtained is provided.

1 is a MIC test result measuring the antimicrobial activity of 1,2-hexanediol
Figure 2 is a MIC test result measuring the antimicrobial activity of the composition obtained in Example 3 according to the present invention
3 is a MIC test result measuring the antibacterial activity of the composition obtained in Example 2 according to the present invention
Figure 4 is a MIC test result measuring the antimicrobial activity of the composition obtained in Example 1 according to the present invention
Figure 5 is a graph comparing the preservative effects of the cypress extract obtained in Examples 1 to 3 according to the present invention and the purified water base solution of 1,2-hexanediol on Aspergillus brasiliensis.
Figure 6 is a graph comparing the preservative effects of the cypress extract obtained in Examples 1 to 3 according to the present invention and the mask pack solution of 1,2-hexanediol on Aspergillus brasiliensis.
Figure 7 is a graph comparing the preservative effects of the cypress extract obtained in Examples 1 to 3 according to the present invention and the O / W emulsion base solution of 1,2-hexanediol on Aspergillus brasiliensis.
8 is a graph comparing the preservative effects of cypress extracts obtained in Examples 1 to 3 according to the present invention and 1,2-hexanediol anionic shampoo formulations against Aspergillus brasiliensis.
Figure 9 is a graph confirming the preservative effect of the cypress extract 1% by weight solution obtained in Examples 1 to 3 according to the present invention
Figure 10 is a graph confirming the preservative effect of the cypress extract 5% by weight solution obtained in Examples 1 to 3 according to the present invention

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

The cypress extraction method of the present invention includes the steps of i) washing the cypress leaves and branches and then cutting them into a range of 1 to 5 cm; ii) mixing 300 to 2,000 parts by weight of polyol with respect to 100 parts by weight of cut cypress leaves and branches and extracting at a temperature in the range of 10 to 50 ° C. for 4 to 30 hours

First, the present invention includes the step of i) cutting the cypress leaves and branches into a range of 1 to 5 cm after washing. The antibacterial / antiseptic component that the present inventors want to obtain from the cypress is contained in the leaves and branches of the cypress, and the leaves and branches of the cypress are used in the present invention. The washing is performed to minimize the content of impurities in the extract, and cutting the cypress leaves and branches to a length in the range of 1 to 5 cm is to pursue convenience in the process such as stirring or filtration after extraction in the extraction step and to increase extraction efficiency. If it is out of the above range, the efficiency of the stirring or filtration process is lowered or the extraction efficiency is lowered.

The cypress that has undergone the washing and cutting process is mixed in a ratio of 300 to 2,000 parts by weight of polyol with respect to 100 parts by weight of cypress leaves and branches, and subjected to extraction at a temperature in the range of 10 to 50 ° C. for 4 to 30 hours. As described above, all conventional extraction methods have a problem in that extraction is performed under high temperature conditions such as high temperature water vapor, and thus pigments and other components are extracted, so that they cannot be used as raw materials for cosmetics. When the present inventors perform extraction for 4 to 30 hours at a low temperature in the range of 10 to 50 ° C, effective extraction of active ingredients such as phytoncide is achieved while minimizing the extraction of components that should not be included in the extract, such as pigments and VOC components included in cypress. It was confirmed that this was possible, and the present invention could be completed. In particular, if the extraction temperature is less than 10 ℃ there is a problem that the extraction efficiency is lowered, on the other hand, if it exceeds 50 ℃ because it can increase the extraction of problem components such as pigments. In the case of extraction temperature and time, it is preferable to perform in the following combination.

The cypress leaves and branches and polyol are incorporated in an amount of 300 to 2,000 parts by weight, more preferably 500 to 1,000 parts by weight, based on 100 parts by weight of cypress leaves and branches. Polyol is used as the extraction solvent applied in the present invention, because the present inventors found that the polyol has a boosting effect when used as the extraction solvent. In the present specification, boosting refers to reducing the activity of water that microorganisms can use due to the inhibition of water activity due to the absorption of water molecules by polyol when using a preservative rather than using it alone or when adding a polyol to the composition of the preservative. It means the effect of helping the antibacterial activity of the preservative by inducing the destruction of the cell membrane by osmotic pressure. When the ratio of polyol to 100 parts by weight of cypress leaves and branches exceeds 2,000 parts by weight, it is difficult to expect satisfactory antibacterial and antiseptic effects in the present invention because the active content of actually extracted cypress is small, and less than 300 parts by weight In the case of extraction, the extraction ratio of active ingredients increases and the preservative effect is excellent, but the smell and color, which are disadvantages of extracts by conventional industrial methods such as ethanol extracts as mentioned above, are too excessive, so that they are not suitable for actual cosmetic compositions. application becomes very difficult.

Hereinafter, the present invention will be described in more detail through examples, which are preferred aspects of the present invention.

Example 1

The leaves and branches of domestic cypress were washed thoroughly with water to remove soil and foreign substances, and then the leaves and branches of domestic cypress from which foreign substances were removed were cut into 3-5 CM size/length. The leaves and branches of the cut cypress were put in 5 to 30% (w/w) of the total raw material weight in a stirring container of 0.1 rube. Then, 70 to 95% of 1,3-propanediol, a polyol, was added based on the total weight. Cypress leaves and branches were well deposited in the polyol, and extraction was performed for 12 hours at an extraction temperature of 30 ° C. while stirring at 20 rpm.

After completion of the extraction, the mixture was immersed at a constant temperature of 30° C. for 6 to 18 hours, aged, and the aged composition was filtered to obtain a final product.

Example 2

Extraction was performed in the same manner as in Example 1, except that 1,3-butylene glycol was used as the type of polyol.

Example 3

Extraction was performed in the same manner as in Example 1, except that 2-methyl-1,3-propanediol was used as the type of polyol.

Example 4

Extraction was performed in the same manner as in Example 1, except that 1,2-butylene glycol was used as the type of polyol.

Example 5

Extraction was performed in the same manner as in Example 1, except that 2,3-butylene glycol was used as the type of polyol.

Example 6

Extraction was performed in the same manner as in Example 1, except that dipropylene glycol was used as the type of polyol.

Example 7

Extraction was performed in the same manner as in Example 1, except that 1,5-pentanediol was used as the type of polyol.

Example 8

Extraction was performed in the same manner as in Example 1, except that 1,6-hexanediol was used as the type of polyol.

Example 9

Extraction was performed in the same manner as in Example 1, except that 1,8-octanediol was used as the type of polyol.

Example 10

Extraction was performed in the same manner as in Example 1, except that 1,2-decanediol was used as the type of polyol.

Example 11

Extraction was performed in the same manner as in Example 1, except that 1,10-decanediol was used as the polyol.

Example 12

Extraction was performed in the same manner as in Example 1, except that butylethylpropanediol was used as the type of polyol.

Example 13

Extraction was performed in the same manner as in Example 1, except that camphanediol was used as the type of polyol.

Example 14

Extraction was performed in the same manner as in Example 1, except that polyethylene glycol was used as the type of polyol.

Comparative Example 1: Manufacturing method of ethanol extract of cypress leaves and branches

Dry the leaves and branches of cypress well to remove moisture. The leaves and branches of cypress, from which moisture has been removed, are extracted with 80% ethanol (a mixture of 99.9% ethanol 80% (w/w) and purified water 20% (w/w)) at 50° C. for 12 hours. In detail, the leaves and branches of cypress were well crushed, 100 g was added to the flask, 2 kg of 80% (w / w) ethanol was added, and then the mixture was well deposited, heated to 50 ° C, and boiled while refluxing for 12 hours. After completion of the extraction, after precipitation, the residue and the filtrate were separated through filter cloth filtration and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain an ethanol extract of Cypress.

The products obtained from Examples 1 to 3 of the present invention and comparative example 1, which is the control group, and the water vapor extract (cypress essential oil, purchased from Cypress Village in Gunsan), which are subject to test comparison, are obtained in color, odor evaluation and prescription, respectively. The diluted color and odor were compared and summarized in Table 1.

division ethanol
extract vapor
distillate
(pyeonbaek essential oil) Representative extract of the present invention Cypress MPO
extract Cypress BG
extract Cypress PD
extract appearance dark brown powder pale yellow oil pale yellow hydrophilic liquid pale yellow hydrophilic liquid pale yellow hydrophilic liquid smell very strong smell strong cypress scent very mild white odor colour raw material APHA Not Measurable (Over)
dark brown every time
Need to be confirmed pale yellow
APHA 50-80 1% aqueous solution APHA Not Measurable (Over)
Brown not dissolved
rated as oil colorless
APHA 3-4

As can be seen in Table 1, in the case of ethanol (or other alcohol) extracts that exist in general powder form, the color is dark due to pigments such as chlorophyll and excessive extraction efficiency, and the smell is strong, so in terms of the content of effective active ingredients Although good, it can be seen that it is very unsuitable for use in cosmetic compositions. Basically, raw materials used in cosmetic compositions are colorless/odorless, and odorless, that is, odorless, which is the recent product trend, is preferred, and even if they are not colorless and odorless, odors that can affect the color and fragrance of the product and colors are rejected by most companies. In this regard, there is a reason why the effect of cypress extract has been known/announced through many papers and patents, but is not commercially applied.

In addition, in the case of steam distillate called cypress essential oil, the color is pale yellow and does not greatly affect the composition of cosmetics, but its excessive specific odor (specific odor of irritating essential oils) is not very good for the steering of cosmetic compositions. The fragrance itself is not suitable for general commercial use because it has strong likes and dislikes. Rather, it is used in very small amounts as a flavoring agent according to the characteristics of essential oils. In addition, in the case of cosmetics composed only of oil, even if used in consideration of the fragrance of cypress essential oil, as mentioned above, the fragrance is too strong and may cause discomfort to the user. In addition, most cosmetic compositions are composed of water, oil, and emulsifier, so in order for cypress essential oil to exhibit a preservative effect, an emulsifier or solubilizer of a certain amount or more is used. , Since the emulsifier and solubilizer of the cosmetic composition are mostly composed of nonionic surfactants, the reduction of the effect due to the neutralization of the preservative effect is also a matter of concern.

In contrast, the antibacterial/preservative composition of the present invention has a very light color and is colorless/transparency (APHA 3-4 based on 1% (w/w) aqueous solution of purified water) close to pure water (APHA color 3 or less) as it is diluted. It can be seen that it is suitable for use in colorless cosmetic compositions such as lotion, toner, essence, mask pack, toner, serum, mist, and extract water. The present invention is particularly preferably applied to cosmetics sensitive to color and odor, and is an antibacterial/preservative composition of extremely light yellow color (APHA standard 100 or less) that suppresses extraction of useful pigments such as chlorophyll as much as possible.

In addition, the antiseptic / antibacterial effect of the cypress extract obtained according to the present invention was tested. In order to measure the antibacterial activity of the extract of the method of the present invention, the minimum inhibitory concentration (MIC) of microbial growth was evaluated for five microorganisms. In order to confirm the antibacterial activity of the sample obtained by extracting the cypress branches/leaves of the present invention with three types of polyol, a minimum inhibitory concentration (MIC) test was performed, and the method is as follows. Each active cultured strain was diluted in sodium chloride 0.85% (w/w) physiological saline solution and adjusted to McFarland standards 0.5 (1.5×10 ⁷ cells/ml) to prepare an inoculum solution. At this time, the number of each bacteria was measured by making a control of the test bacterial solution and the exact number of bacteria actually introduced through the 10-fold serial dilution method. The three representative extracts and 1,2-hexanediol of the present invention are four substances (three extracts) to be measured by applying a 2-fold serial dilution method using each medium. , 1,2-hexanediol) at 5%, 4%, 3%, 2.5%, 2%, 1.5%, 1.25%, 1%, 0.5%, 0.25%, 0.1% (w/w each), respectively. The prepared liquid culture medium was used by preparing 10 ml each in a test tube. Three species of bacteria were cultured in an incubator at 37 ° C for 24 hours, and fungi were cultured at 25 ° C for 72 hours, and then the minimum concentration that inhibited the growth of each strain was confirmed to determine the MIC. The results are summarized in Table 2 below.

Minimum Inhibitory Concentration - % (w/w) division Escherichia coli
ATCC 8739 Pseudomonas aeruginosa
ATCC 9027 Staphylococcus aureus
ATCC 6538 Candida albicans
ATCC 10231 Aspergillus brasiliensis
ATCC 16404 1,2-Hexanediol Company A in Korea 1.25 1.5 1.25 2.0 4.0 Cypress MPO
extract 0.5 0.25 0.25 0.25 1.0 Cypress BG
extract 0.25 0.25 0.25 0.25 0.5 Cypress PD
extract 0.25 0.25 0.25 0.25 0.5

In addition, the antibacterial effect of the extract according to the present invention and each comparative substance was compared and measured. The measurement followed the Disk test method, and Aspergillus brasiliensis ATCC 16404 was adopted as the inoculation strain. Accordingly, after inoculating 5 μl of the stock solution, the results are shown in FIGS. 1 to 4. As can be seen in Figures 1 to 4, it was found that the cypress extract composition according to the present invention has an effect of up to twice or more compared to the composition in which 1,2-hexanediol is added to cypress essential oil.

In addition, the antiseptic effects of 1,2-hexanediol and the composition of the present invention were compared by preparing or formulation in various forms. Tables 3 to 5 below show 1,2-Hexanediol 1%, 2% (w/w) and three compositions of Examples 1 to 3 of the present invention (using a representative polyol for cosmetic use) each 1% (w/w) purified water This is the result of comparing the antiseptic effect of the aqueous solution base.

Among the results of Tables 3 to 5 above, the preservative effect results for ATCC 16404 are summarized in FIG. 5. As can be seen in Figure 5, it was found that the extract obtained according to the present invention showed a much better preservative effect compared to 1,2-Hexanediol, which was used as a conventional preservative.

In addition, the antiseptic effect of the mask pack solution base was evaluated. In Tables 6 to 8, 1%, 2% (w/w) of 1,2-Hexanediol and 1% (w/w) aqueous solution of purified water of each of the three compositions of Examples 1 to 3 of the present invention are used as mask pack solutions. This is the result of comparing the preservative effect.

In addition, Figure 6 is a comparison graph of preservative effects for Aspergillus brasiliensis ATCC 16404 in Tables 6 to 8 above. As can be seen in Figure 6, it was found that the extract obtained according to the present invention showed a much better preservative effect than 1,2-Hexanediol, which was used as a conventional preservative.

In addition, the following Tables 9 to 11 show 1%, 2% (w/w) of 1,2-Hexanediol and three compositions of Examples 1 to 3 of the present invention (using a representative polyol for cosmetic use) each of 1% (w/w) ) in the form of an O / W emulsion, and then the preservative effect was compared.

In addition, Figure 7 is a comparison graph of preservative effects for Aspergillus brasiliensis ATCC 16404 in Tables 9 to 11 above. As can be seen in Figure 7, it was found that the extract obtained according to the present invention showed a much better preservative effect compared to 1,2-Hexanediol, which was used as a conventional preservative.

In addition, Tables 12 to 11 below show 1%, 2% (w/w) of 1,2-Hexanediol and three compositions of Examples 1 to 3 of the present invention (using a representative polyol for cosmetic use) each of 1% (w/w). ) is added to anionic shampoo and then the preservative effect is compared.

In addition, Figure 8 is a comparison graph of preservative effects for Aspergillus brasiliensis ATCC 16404 in Tables 12 to 14 above. As can be seen in Figure 8, it was found that the extract obtained according to the present invention showed a much better preservative effect compared to 1,2-Hexanediol, which was used as a conventional preservative.

In addition, 2-methyl-1,3-propanediol, 1,3-butylene glycol, and 1,3-propanediol, which are polyols used as representative moisturizers in cosmetic compositions, are antibacterial/preservative when used at a certain level or higher, respectively. effect is manifested. In general, according to the introduction of each manufacturer, the effect is about MIC50 to 60 at the 10% level, so the preservative effect under the purified water condition is measured at the 1% and 5% (w / w) levels, respectively. The confirmation of whether this synergistic effect was carried out. The test result was to confirm the tendency of the reduction effect for Aspergillus brasiliensis. The results are shown in Figures 9 and 10. As can be seen in Figures 9 and 10, the antiseptic effect of each polyol on Aspergillus brasiliensis shows a certain level or higher under the 5% (w / w) standard, but the 1% (w / w) of the present invention /w) results are very poor.

As described above, it can be seen that the cypress extract extracted according to the present invention can obtain odor, solubility (hydrophilicity) and color suitable for use as a raw material for a cosmetic composition. In addition, it is useful for more general-purpose commercial use by using polyol, which is a humectant almost indispensably used in cosmetic compositions, as an extraction solvent.

One embodiment of the present invention described above should not be construed as limiting the technical spirit of the present invention. The protection scope of the present invention is limited only by the matters described in the claims, and those skilled in the art can improve and change the technical spirit of the present invention in various forms. Therefore, such improvements and changes will fall within the protection scope of the present invention as long as they are obvious to those skilled in the art.

Claims (4)

i) washing cypress leaves and branches;
ii) cutting the dried cypress leaves and branches into a range of 1 to 5 cm;
iii) A cypress extraction method comprising the step of mixing 300 to 2,000 parts by weight of polyol with respect to 100 parts by weight of the cut cypress leaves and branches and extracting at a temperature in the range of 10 to 50 ° C. for 4 to 30 hours. According to claim 1,
iv) Cypress extraction method further comprising a process of aging at room temperature for 10 to 30 hours after extraction. According to claim 1,
The polyol is 1,3-propanediol, 1,3-butylene glycol, 2-methyl-1,3-propanediol, 1,2-butylene glycol, 2,3-butylene glycol, dipropylene glycol, 1 1 selected from the group consisting of 5-pentanediol, 1,6-hexanediol, 1,8-octanediol, 1,2-decanediol, 1,10-decanediol, butylethylpropanediol, campanediol and polyethylene glycol Cypress extraction method, characterized in that more than species. A cypress extract extracted by the method of any one of claims 1 to 3.

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