KR102461763B1 - Snp marker set for identifying cucurbita maxima cultivars and method for identifying cucurbita maxima cultivars using the same - Google Patents

Abstract

The present invention relates to a single nucleotide polymorphic marker set and its use for backcross generation-shortened breeding of Western amber. It can reduce the time and cost required for judgment or increase the accuracy of judgment.

Description

SNP MARKER SET FOR IDENTIFYING CUCURBITA MAXIMA CULTIVARS AND METHOD FOR IDENTIFYING CUCURBITA MAXIMA CULTIVARS USING THE SAME

The present invention relates to a set of single-nucleotide polymorphic markers for backcross generation-shortened breeding of western squash and uses thereof.

The backcross method is a breeding method used to introduce useful traits of a particular variety into other varieties with excellent practicality. Finally, it consists of a process of selecting individuals that are similar to the recurrent parent but have useful traits of the introduced parent.

Conventionally, in order to select an individual having a desired trait, the phenotype of the backcrossed individual was confirmed. However, there was a problem in that it took a very long time because the individual was grown, the phenotype was visually confirmed and selected, and the generation was repeated until an individual with the desired trait was obtained.

Recently, in order to solve this problem, a breeding method using molecular markers that can select a desired individual at an early stage by identifying the gene at a stage before the growth of an individual is being developed. Marker assisted backcrossing (MABC) can be accomplished by selecting a backcross offspring that retains the target gene of the introduced parent, but whose parts other than the target gene have the genetic makeup of the repeat parent.

To this end, it is necessary to secure molecular markers that are evenly distributed throughout the genome, compare the molecular markers of the recurrent parent and the backcross progeny, and select backcross progeny with high similarity to the recurrent parent. It should be relevant. SNP is a molecular marker that can automate the entire process from detection to analysis, and has many advantages over other molecular markers in genotyping.

Although a set of SNPs for backcrossing using molecular markers for various crops have been developed, molecular markers that can actually help select backcross progeny for Western squash (Cucurbita maxima) are not known.

KR 10-2125528B1

One object of the present invention is to include an agent for detecting all single nucleotide polymorphism (SNP) located at the 61st base of each base sequence in the base sequence of SEQ ID NOs: 1 to 240, between varieties of Western pumpkin To provide a composition for identifying differences in traits.

Another object of the present invention is to provide a kit for identifying trait differences between varieties of western pumpkin, comprising the composition.

Another object of the present invention is to a) isolate a polynucleotide from a repeat parent western amber sample, and determine the genotype of all single nucleotide polymorphisms present at the 61st position of each nucleotide sequence shown in SEQ ID NOs: 1 to 240 to do; b) isolating polynucleotides from backcross progeny Western pumpkin samples, and determining the genotype of the single nucleotide polymorphism in step a); c) comparing the single nucleotide polymorphism genotype of the repeat parent determined in step a) with the single nucleotide long polymorphism genotype of the backcross progeny determined in step b); and d) selecting a backcross progeny having a high ratio of matching repeat parent and single nucleotide polymorphism genotype among the backcross progeny.

One aspect of the present invention is between varieties of Western pumpkin, including an agent for detecting all single nucleotide polymorphism (SNP) located at the 61st base of each base sequence in the base sequence of SEQ ID NOs: 1 to 240 A composition for identifying a trait difference is provided.

In the present invention, the identification of trait differences between varieties of western amber may include not only identifying the cultivar of western amber, but also identifying the cultivar of western amber. The identification of trait differences between varieties of western amber may be identification of trait differences between western pumpkins. The lineage refers to individuals belonging to the same breed, but which can be classified by differences in detailed traits. The composition for identifying trait differences between varieties of Western pumpkin can be used to identify or determine similarity to one or more of Western pumpkin varieties, strains, genotypes, phenotypes, traits, and combinations thereof. The composition for identifying the trait difference between varieties of Western pumpkin may be to identify the genotype of a selected single nucleotide polymorphism by detecting or amplifying a polynucleotide including a single nucleotide polymorphism site.

The nucleotide sequences of SEQ ID NOs: 1 to 240 are as follows:

SNP Marker Name Chromo
some Genetic distance (position) Flanking sequence (downstream 60bp, upstream 60bp) SEQ ID NO: CmaMAB001 One 187112 AGAAAGATGGACCTGAAGAAGGGATATTGTCTGATGTTGCCTTGCGTGCTCCATCTTGTG[T/C]TTCATCAAAGCATAATGAGAAGCTAAATCCTCCTGCACTATCTGATGATCCAGAATCTGG One CmaMAB002 One 616755 ACCATGTTCTAGGCGTCTCCTCAAGAAGAAAAGACATACCCATCCATCAAAATCAAGATC[T/C]AAGTCATGTTCTTGAGAAAATAGCCACTGCCCATCAATAACAAACTGGAAAACCCGAGAA 2 CmaMAB003 One 1166024 TAGTTGTACTTAGTTTGTGTGCCCCTTCTACAGGGAAATCAGCATGTGCAAATGGCTGGA[A/G]ATGCTTCTCATGGGAGTCACGTGGATGCTTCTGCTAAAGGTGAAACGTACGGCCAGCAGC 3 CmaMAB004 One 2025448 GCGATCATGGAGTCAAATGCTGAAAACCCCACAAGTTTAAACCTATAATGAATTACAGTG[A/G]ACAAGTTTCAAACTGCTCTTCTTTTGCAGCAATGGTGACCCCCATTTTCGTTCATGTCAA 4 CmaMAB005 One 2366000 CACAAGAGGACACCAGAAATTACCAAGTTCAACTTGTACGCACTTACACAAGAAGAGTTT[A/T]TAGGGAAAGAAAATGGGACACTAGCCAAAACAAAACTTATTTTACCTGGGGCTGCAGGGA 5 CmaMAB006 One 2716186 CATACACCTCTCGGCTCCATTCCAAGTGCCTCATGTCAGCTTCAGGGGATTCCCATGTAA[T/C]ATAGTAGCCAATTTTTATGGGATACCCTGCTCGATGCGGCGAAGGGAGCTCTGTTTCAGA 6 CmaMAB007 One 3175414 GGAGTGAGTCGTCATCTCCTACCCCGATTAGGCAAAAGGACCCGTTGCTTAGGAATGTC[A/G]ATTTATTCATATTATTGCAACTTTTTCTTCAGGGGGGCTGTATTTTCAACCTCTTTCGTG 7 CmaMAB008 One 5789572 TCGGGTAAGTGATGGCGAGTTCTTGGAGATGATGGCCTCTGATGAAGGTGGCTCTTGACG[T/C]AGTTCCTTGACAACAGCAGCTAGAGCCTCCGGATTCACTCCCATGTCACAAAGAGCAATA 8 CmaMAB009 One 8031509 AATAGCTATGGTTGCAACAAAAGCAGCAATTGAAGCGAGACCAAGATTCTTATACAAAAC[T/C]AACAGGGCCAGTACAACTTGAAAAACCACCAGCCAAACATCATGCATATAACCAACTGAAC 9 CmaMAB010 One 8540764 GCATATTATTGAACTTTCCTGCTAACCTCTAATTTTGGACAACGATTCAGTCCTTGTCAT[A/G]CTTATTATTAGTTTTTCTCCAGGAGTTGATGGTGGGTTTGATAGCAATGAACCTGAATAT 10 CmaMAB011 One 8676781 AAGTCAAGGAAGTTCAGAGAACAATCAAGGTATGATATACTATGAATCTACATATGTGCG[A/G]TTTTTGCTGCCTAAATTCACATATAAACTATACTCTAGTACATTGTCTGAAACTCGATAT 11 CmaMAB012 One 11069490 CCATCCCTTTTATCTGTTGCATCAACACCTAATACATTTACAGCAGCGAAAGAGCAAAAC[T/C]CAAAGAGGCAAAATGATTTCTTTCATTACAAAGTTGTTCTTTATATCTACAAATGTTCTT 12 CmaMAB013 2 130733 GACGGGACATGGCATTGTTAGAAGCGGCAAGAGGGCGTCGGACGGGGAGGGGATGAAGGG[T/C]TGTCCCCCGGCAGCCAAATCGACATGATGATTGGGTCTAATGCTACACATTCCAGAGTCT 13 CmaMAB014 2 902391 CTGTTTGACGAGAATGTTGTGAAGTGTTTCTATCCGACTCCGTCCGATGAAACAAGGGAG[G/C]TTTTGGTTGTGTTGCCTGTCGGAATTGGTGTGTTGTGCAGCTTCTTGTTTATTGTTTTTC 14 CmaMAB015 2 2444178 ATGTTTTGAGAAACAAGCCTTACAGATATCATCATTGTTGCCACAATTTTTAAATGAAAA[A/C]TATGATCGAACAAACGAAAAAAACAGCTATCTTAATATGTTCACAAGAGACTCAGACATC 15 CmaMAB016 2 3955016 TCGGCTGCGACGGCAACGGCGGCGACGGAAAAGCGAAGAGAGATTGACAGAAGGAAGAAA[T/C]AACCGAAGGGGGAGGGAGAGAAAGAGAGAGAGGTTTTATAGTGAAAATTAGTGAGTGTGG 16 CmaMAB017 2 4510484 GGCAAACGCCGGTCCAGACACAAATGGCTCACAATTCTTCATCACTACAGTCACAACTAG[T/C]TGGTAAACTCCTGTCTGCCACGATTTTGTTTCATGCTTGATTTGATTGATTACTAAAAGG 17 CmaMAB018 2 4534199 AATCTCATACACTTCCTTAACGAATTGCTGGCCTCGCGCCACGTCGTGCGACGGATGGTC[A/G]TCGTTCGCCGGAAAAAGATTGACGTCGAAGTAACGCTGTTCAATCACTGCGTAATTTGGG 18 CmaMAB019 2 4768136 CTTCCTTATGGCAACAGTCTTACCCAACATTTCACCCTTGTAAAGACAACCATATCCTCC[A/C]TGAGCAATCTTGAAGCTTTCAGAGAAGCCACATGTGGCTGTTTGTAGTTCCAACAATGAA 19 CmaMAB020 2 4768207 TGAAGCTTTCAGAGAAGCCACATGTGGCTGTTTGTAGTTCCAACAATGAAAATTCAGCCA[T/G]CTGAGTTTGATTCTCCACAAATCCGATCGATCTGTTATAATTTGCAGAAGCAGCCTTCTC 20 CmaMAB021 2 6827034 CTCAATGGAGCACAACTCATGGAAGGCACTATTGCTGATTCCAAGATCATGCCCACACGA[T/C]TGTTTTGAACTCTGAGATCAATGAATTTTCAATCGTCCCACTTTCTGGTGCAGCCACATAA 21 CmaMAB022 2 6904099 GTTATTCAACGCCTCCATTAGTGTACTCATCATCGGTGGCAGCAAATCCTGAAACCTGTC[A/G]CGATCAGCAGAGTTCGACAAGCACTGTATGAAGCTGATAACAGCATTGAGGGCCGCAATC 22 CmaMAB023 2 6916002 GTAGAGTAGCAGTTTTATCATAACCAAGTAGGCTGTGGTTTGAATTAAAATTTTCAGGTC[G/C]TGTTGATGAAGCATTCCTGCATCCATCTAGGTAATCTCTACCGATTGCAGCTTTTTCATC 23 CmaMAB024 2 7060897 AAGAAACTCTGGAAAATCGATAGTTCCATTTCCATCCGCATCGACTTCGTTGATCATGTC[T/C]TGCAGCTCAGCTTCAGTTGGATTCTGACCCAATGACCTCATTACAGTCCCCAGCTCCTTG 24 CmaMAB025 2 7466537 AAGCCTTTGGATTTGATGAGGGAATAGCACCCTCATGTGTTAAATACTGAGAAACTTCTG[T/C]ATCATATGGATTTTCACTTTGAGATAATCCCCTATCTTTTAAAGACGAGCTGCTTTCCGA 25 CmaMAB026 2 7613635 CCTACAAAAACAAATGGCTTGGTTGACATATGCAAGGAAATATCAGATCTTACCTGCGTA[T/G]TCGTTGTCCAAAGAGTTCTCATCGTCACTTTCTGCCAATGTATCTCCACCAGTGCCCTCG 26 CmaMAB027 2 7661474 TGTTGGCGAGGAGGGAACTCCGGGATACGTTGGACCGAGGTTAATATTGTTTGGTGGTGC[T/C]ACTGCGCTCGAGGGCAACTCTGCTGCCTCAGGAACTCCATCATCAGCTGGAAGTGCGGGA 27 CmaMAB028 2 9808140 GCACTTGCCCACCAACAAACTATGTTGTATGTTTGACTCATAACTTATTCTACACGTTCTT[A/G]TAAACCAAGCACCAGAGTTTAACGGCGTATATTATGTTGTCTGCATGATACAGCAATGAT 28 CmaMAB029 2 9968283 GTCGCACATTCTTACGCTCCCCATCATTGCAGCAAGGTGGAGAGGATTGTTTCCCCTCTC[A/G]TCTGTAGTCTCTAGAACTTTCTTCTTAGTAATTGTGTCTTTGGTGGCTTCGCTTATGTGT 29 CmaMAB030 3 1152786 GCTTGTGCATGCTTGGTGCGGTGGTGATGCGAGCGTTTCTGCTCTGTGAGGATTTGGGGT[A/G]TTGCAGCTTGCTGGCTTCTTTTGCTTTCTCCTCCTTGGATCGGTAAGATTCTTCAAGTTT 30 CmaMAB031 3 3701046 GGCAATACCTACGAGACCAACTACCGGAGCTGCGACGAATAGCCAACTTCGTCGACCTCT[G/C]CCTGAATTGTAACTATTACTAACAGCAGGGTCTATGTTGCTCAGCTCCTCTCCTTGGCTG 31 CmaMAB032 3 4013234 AAGAAAGAGACCGACCGAGAGGAGGTGGCAGAAGGGGTGCAGCTGAGAGCAGGTGGTGCC[G/C]TTCAACCGCTCTCTCACCGATTGCATGACCGCCACGACTCCTCTTCTTCAAATCCCAACC 32 CmaMAB033 3 4592809 AATGAGCTTCATTTCCTCCAAGACGCACCTCATGTACTCGAATGATCCTGCCTTTCCAAG[T/C]TTGCGAAGAATCTCCTTGTAGAACGACGAACTAGGCACGAAGTTGGGCTGCTTCGAAGCC 33 CmaMAB034 3 4959072 AACTTCTGTGGAACGATCAGCAGCATTAGATAACACATTGGAACGAGGGATTGATAAGTC[A/T]CCTTGTCGTCCATTACCTACATGAAATATCCAATTATTTCCTGTCAAATATACATTCACA 34 CmaMAB035 3 5517619 TGTCTTGGGCCCAACTTCCCCCTTCAATAGTTTATTGACTATACGAATGTTGGCGAACGT[T/G]CCACGTGCCATAATCTCGTCATTCCCACGGCGACTACCGTACGAGTTAAAATCTCTTCTG 35 CmaMAB036 3 5694907 TCTCACCCAAATAGACAAAAAGTTCACTTTTTTTCGTTAAATCATATTGAATCTTAAAAG[A/T]GCTTACTCTGAAACTGTCAGTGGAAGACCCATATCCGTAATTTCCCATATGCCCCACGGC 36 CmaMAB037 3 6622370 CGTCAGACGAGTTTTTGGGCTTGACGGCCCCAAAATTCTCCTCCAAGAACCTTTCCATTT[T/C]TCTGAGTAAGTTAGTGAAAATAAACTTAGGTATCCGCAGTTATATAAAGAAAGGAAACAA 37 CmaMAB038 3 8152745 AGATACACCGCTCATATAACATAGACCACTGAAAGCCTTGAAGACACATAAATTATACCT[G/C]GGCAAGACCTCTTCCAGTGACCACGCCAATGCTAGCCGAGAGATACTGGCACAAAATAGC 38 CmaMAB039 3 9288270 TTGCTGCTCAAAATCATTGAACAATTGGATCAGATGTTGAGATTCTTTTAGAACAGATTC[T/G]TCTAATTTTTCCCAAGTGCATTCGTCGTAAGGAAGGCCACTCCATTTTATAAATGCTTCA 39 CmaMAB040 4 304877 GAAACTCACTGCTTTCATCCCACAACACTCTTTTCTGAGTCGGCATTCCTGCATTGTCGGC[T/C]AAGGTTTCAAATTCCGACCTTGTTCGCCTCTGATGAAGCAGCAGTTGACAATGTCGTGAA 40 CmaMAB041 4 424789 TAATTTTAAATAAATAAAAAAAATATTTAAGTTGTTTGATTAGCTGCAGGTTGTTGCCC[A/T]TGAAACGCTAAGGAAGCATGAAATTGAAGTATTACCAAAGATTGATCTGCGTTCTCTTCT 41 CmaMAB042 4 2574422 TGGAAGGTTAGCCCAGCAGCCATTCTGAACAACAGGAGGCTGGTCATGTAGATCGTTTAA[T/C]GACGATGCATTGGACTTTCCTCTATGATGACCACTCAGCTTGACCTCGAAACCCCGTCTA 42 CmaMAB043 4 7609577 TTGATTGAAAGGTTACTTTCTAGAATCTTTCTTTGTCGTTTTATCCGAGGAGTTGCAGAA[T/G]TTTCCCCTTTTTCATCATTATGTCGTTTTATCTTGACATCTAGGTTCTTCCACTTGCTC 43 CmaMAB044 4 7660660 CTTCAAAGCCTCTCCGGCTATGGCCATGAGATCCTTGGAAAGCGAAATTCCGACTTTGCC[T/C]TCTTTGTCCTGCTCCTCGAAGACGCAGCGGTAGGCCTTGTCGTCGGCTCCTTTGTGAGTG 44 CmaMAB045 4 8357591 GCAGCTAGAAAGCAGTTCCTAGAGACAGGATAATGGAGAGCCAAAACCAGGTCACTCTCA[G/C]AGGTGCCGCTTGAAGGCACCACCTTCTTCGCAACTGGTATCAAATTTGGGAGTCTAAAAT 45 CmaMAB046 4 8680104 TAGAAAAGAAGACCTCTCACAGTTGACATGTTACCTGTATAGTTATCGCAGCTAGAATCA[A/G]GCTCAGGATCACGTTCAGAATCCATAACCTCAACCTCATTAGCGGATTCTTCAGCAAGAA 46 CmaMAB047 4 8943024 CCTGCGCACCCAAATCAGCTGTTGACGATGGAGCCTCAATGCATGTCTGTAGTTTAGTCA[G/C]AATGGAAAGCAGCTCTCATATTATATATATGCACATCAGAATCTCGATAGCTCGAATATT 47 CmaMAB048 4 9276347 ACGCCCACATCAACGAATCGTGGCCCAGCCCGAACACCAGAAAATTGCAGGGACATCGAG[T/C]CTTCAATACGTCGAATGAGATACTGATCTCGTCAAACGATTGCTGCGGAACAATCTTCGA 48 CmaMAB049 4 9512341 AATGTTTGAGACAATTGAAAGGAAGCCAAGCATTGACGTCTATGATCCAAAGGGTAAAAC[A/G]TTAGATGATATTCAGGGAGACATAGATTTGAAGGACGTGAGTTTCAGTTATCCAACCAGG 49 CmaMAB050 4 9900308 TTATGAGTTGCTAGCATAACCAAAAGAACTCTTGGCTGGATGTTGGAATGAACATGAAGC[A/T]AATTGGGAATGGTTGCTGCAAGGGGGATTCTCAACAGAAGTTGTTTAGTAGGGTGCATTT 50 CmaMAB051 4 17524149 TACCGGCCAGTTCTTCAGGTTTCTGATTGTTATTCATACGGACGATCAAATATGAATAAA[T/C]AAGTCTAAATGGGTTTCTGTCCTGACATATTTTATGGTTTCTGCAGTTCACAGATAAGGT 51 CmaMAB052 4 17949920 GTGTCTCTTCTCCAGGCTCTTGTGGCCGAAAAACAGATTTTACAGCATTTGTTGCACGTA[T/G]ATGCTGCCCACTTAAACTCAGCTTGGTAGAATAAGAACCAATGGTTTTCATGCGCTTTGC 52 CmaMAB053 4 19425778 AAACTTCTACTCGATAACCTGTTGTTTTTTCTTACATCATTGGTATGCTTAGCAGATTAC[A/C]ACTTCTAAAGTTGTTTTTTTAGGACTAAGCCTGACCATTGTGCTGCAGGTTAGTGTACGG 53 CmaMAB054 5 347527 TAAAATTCGGCAAAAAATTCCTCACTCATTACATGTCTCGCTGCGTCTCTCTAATTTCCA[G/C]TACTTTTTGAATCTGTTTTGTTGTGGTTGCAGGGAAGGATATCAAATCTACCATTTGAGT 54 CmaMAB055 5 561710 GTTTAGCTTAGTCATGAAGAGTCAAGCAAAAAACGACAGGGCGGGATGGAGAAGTCATGA[A/G]GGTTAAAAAGTTGAAGGGAGAACGGCATGGTGTATGGTTGAGCTCATGCCTTTGCTGCAA 55 CmaMAB056 5 1077724 ATTTCTGAGTACAAGAAGCCCAAAAGCTCCTAGTGATTTGGTTGGCTCTGAATAGCGCGA[A/G]GTCAGAGAGGAGGAGGAAGAAGAAGACGATGGGAACTCTGGGGAAAGCCATTTACACCGT 56 CmaMAB057 5 1245092 TTGATCACCTCCGACACACGCATCGGAGGACTGGATTTGGTGCCGACCATTTCTTGAATG[A/G]TTAGTTAAATGTGAGGCAGCTTTACCTGGAACATCAATACCTACAAATTTTACCGGTATA 57 CmaMAB058 5 1327012 AAATAAGAACCGCGGATTGACGGTTCGGTCTGCTCCGTTTGAAGGTTTGCGATTACGTGC[T/C]GGTGACGGCCGGCGGAGGCCGAAGAGGCTGGCGAAAGCAAAGCCGGAGGGGGCTGCGGAG 58 CmaMAB059 5 2395422 TTTAAATTGGTGGAATTGTACAGGACGAAATCAAATTGGGAATCTTCATACTACGAGTGC[A/G]GCTGCTGCGTCTTTCGGTGGGTTACTGACGCCGTATCCTCCGCCGCCTTCGTCGCCGCCG 59 CmaMAB060 5 3721308 GATTGAAATCCAACAAATCCTAGACTTGTTGATTGATGATCTGAGGAGGGAAGGTTATAT[A/T]CCTAATACCACTCTTCTTTAGCTAGACTAACTTTCATTCATGCTCATTGTTTTTGTTCTGT 60 CmaMAB061 5 3786225 AAAACTGAACGAATATCCACAAAGAAGAACTTTTCCTTCAACCAAACGTTGTTTAGAAC[A/G]TCAGGCCTTTGGCAGTCTGAAGGGCTGTACTTCGATACTGAAGAATCTAACAGAACGTCA 61 CmaMAB062 5 5770482 CGGCTGTATGAAGAGAAGGATAAAATGCACGCACTGAACGGAATGGAATACTCATTTGCT[A/G]TCGCTGCCGCTTCCTTCAGGACAGCATACAGTCTGAACAAGACCTTGTATCTTTGGTATA 62 CmaMAB063 5 7716159 GGTCGATAGAGAGACGGGGAAGAATCGACTTCTAGCCTCTGCCCTAGGCTCCATGCTCGT[T/C]GTCCACCCACAGGTATTCCTCTACCTCAACCTCGATTCCATCATTCTTTTGAGAGTAGTA 63 CmaMAB064 5 8558222 AGCTTCGTGTTGCAGAGGTCGGAATGTTTTGTTTCCAAAAATAACAACATTCGCCAATTC[A/G]AAATCATCCAAAGTCTGTAACTCTTCATAAGTAAGAGTTTTGTGCTCAACCCCTGAAGCT 64 CmaMAB065 5 8781968 ATTTCGCAGCTTGAGTAGGAAAGCTAAGATAATGTTGGGTTAACTCAGTCAGAGATATGA[T/G]GTGGGACATTTGGGGATGACAGATACTCTACAGCTTTCGTTGAAAAAGATTAGAGTGGTT 65 CmaMAB066 5 10254570 AGATACTTCTGAGATGAAAGGGGAGTTCGAGCATATTAGAGAAAAAGAGAAGGATTTATC[A/G]AAGGAGACAAGGGAAGAGAGGGAAGAGCGGTTGCAGCGTGAGAAGATTCGTGAGGAACGA 66 CmaMAB067 6 533452 CTCATCTGTTGTGGAAGGCAAGAATACGTGGGAACCGTGGTTTCGATACTTCACTTTCTAG[A/G]AATGGTCTCTATTCTGGTGGAAGGTATTTTCCAACTGGTAACCATATTACTCGGTATTTG 67 CmaMAB068 6 597363 TTTCATTGGAGAAGGTTCTTACGGTCGGGTTTATTATGCAACGTTAAAGTGCGGTCAGGC[T/C]GCTGCAATAAAGAAACTGGATACCAGTTCTTCACCGGAGCCCGACAACGATTTTGCAGCT 68 CmaMAB069 6 685756 AAGAGAAATGCGGTACTCGGACTTGAAGAAGATCGACCGTGGGGTTCGGCGTCATTTTCA[T/C]GACGACACAACGGTGATAGTTGTGTTTCTGGGTTCGAGTGGTTCAGGAGGAAGGAGCCCT 69 CmaMAB070 6 1020022 AGATTTTCCCTTTTGATTCAGCCGTTCTTATCTTAGCCGTTCTTCTTTCAATCTTCGATA[T/C]TGTTCTCAAAATCTCAGCCTTCGCTGTTTCCCCCATTTTCCTAAACTCCTCAGCTTCAGA 70 CmaMAB071 6 1089167 GTTAAGTTACACTTGTAACTAATATACTTACTTTTGTTAACTTTATGCAGCTCGCGGGCC[A/G]GAAACGACAGTCGATTGGGCAACAAGGATGAAGATAGTGATTGGAATTACGCAAGGCTTA 71 CmaMAB072 6 1290229 CTGGTCCGAAGACACCTCCTCGTGCAAATTCCTGTCAAAGTGACAAGTCTACATCTCCAC[A/T]TGAGATTTCTTCTGCTGCTGATTGCAGTAACAACAATACTCCACAAGACGTTAGTCCTAC 72 CmaMAB073 6 1916750 CGGTGTGCGGCCACTTTCTTTTGTTAAAAAGGAAACGTGAGCAGAAAGCAGGACATGAAG[A/T]AGAAAAAAAGTGATAAATGGAGGCAGCACGAACAAATTCAGTATCTTCCAGGGGATATA 73 CmaMAB074 6 2419031 GTTGAGAGCCAAGTTGAAATGAAGCTGATTATCGGTGAGGTTGAATTGGGTGAGTTTAGC[T/G]TCGGTGACGTGGAATTTGACTTTGTTGGGACGGAATATGAGCCATAAGATCAATACGGCG 74 CmaMAB075 6 3347772 ACTGCATTTGTCTGATCATCTAGCCTTTCATTTAACCCTGAAGATTTTCTTTTTCACCAAC[G/C]CATCCGACTTCATTACTTGCTCACTAACATTGGCAGCCCTCGTCAGGCTTTCTAGAGCCT 75 CmaMAB076 6 3464440 GCTCAACCGATGCTGCAACCCCCTCTGGTAGCTCGACTCGGGCCACGTATGTCCCTGGAC[A/C]TCCCACATTCTTAACTCTTCTATTGATTGTCACCGGAGCATTCACATTTAGATTTGGAAT 76 CmaMAB077 6 3465647 TCTTCCCTGCAGTGCAAATCAAGATCTTCCCCTTCACCTTCTTCGGATTAAGCGTTCCCT[T/C]GCCGCAAAATTTCCTGCAATTAACGCCAATTTCAATTTCATAATCATCGAAGCAGCAGTT 77 CmaMAB078 6 3613604 AGTGGTAGCTGCAGCGAGTATTTTGCTGTAACCTCTTCAATTCAACAGAGATGTTGCCTT[T/C]ACTTCAGCCAAAGTTTATGCATCTAACTCTTCAAGCAATAATATCATAATGGGAACTCCT 78 CmaMAB079 6 8843987 TAAGGGCACGCTAACCTAATAAAGCGTTTCAATAGAGTTCCAGGAATCTAGTTCCATACA[T/C]AAAAAGGATGTCCTCAGAATCAAGTGTTACTTGATCGTAGAGACTCCGAAGAAAAAGCA 79 CmaMAB080 6 9531280 TGTTAAAATTCATCAGTTCAGTCCAGTTTTTGAACAAAAATGAGAAGAATCAAGGCAATA[A/C]GCAAGAATTTTTGTACCATCTTAGAGACACAATTGGAGATTGGAATGGCGGCTTTGGCTC 80 CmaMAB081 7 250698 ATATCATATGCAAAGCAAAACCGGTTCGGATGCTTACCGAGAAATTCGACAGAGTCGGTG[G/C]CAACAGAGCTCCAGGCAGCCCATATGAAATTTCCAGGAGCTACCAAGTTGGGCTTCATAA 81 CmaMAB082 7 260780 ACCGCTCTTACATTGCCCTTGGCAGCTATCAGGTCTGTTCGTGCACATTAGTGTGGTTGT[A/T]AAGTATATTGGTGATTGCTTTCAACTTGATTTGATACTCTGTCGTACTCTTTCAGCTAGT 82 CmaMAB083 7 260916 CTCATCCGCCCCCACGCCTCTCCAAGCCGTCAAATTGCTCGCTCTTTATCTGTCTGATCC[T/C]AGCAGCAAGGTTTCATTCGTGGATCTATATCCTTTTTAGCTGATTATGTTCGTTTGGAAT 83 CmaMAB084 7 373716 TGCCTCACTTTCTTCATTTTGAAAGCTATTGCATGCAGCAGGTTGGTCTTCTTTTTCTGT[A/T]TCTGTTTCTTACTGTATAATGGATTATTTGGCATTATATATAACCCAGCAGTACGAACCA 84 CmaMAB085 7 2090872 CAAAGCAAGCTTTATTATAATACTGCTTAAAAAGTAGCCATTACTGCATCAAAATAAGCA[G/C]AAAGGCAAAGAACAGATAGAGAGGAGATGAACACACTTTTATAAGGCCACTGGCTTTGTC 85 CmaMAB086 7 2523636 GAAGGAGCGCCATTGATGAGAGCTCAAGCTTCCAAATCCATGGCTGTTGTGCTTCTCTTTT[T/C]GCCTTTCTTCTCCATTGGCTTCTTTCTGTTGGGGCAGTCAATAGCTCCATAACGTAAAAA 86 CmaMAB087 7 3324630 TCGGTCCGGTGTAGGGTTGCTGAGAAGCAATTAAAGAGAAGAAGCAAAACAGAGAGGCGA[T/C]GAAGAAATGGAATTTGAAGGAACCCATTGGAAAATAAGAGAGATTTTGGAGATGGGATTC 87 CmaMAB088 7 5112390 CGGTGGTTCAACCATGTTCCCTGGCATTGCTGATAGAATGAGCAAGGAGATCGCAGCGTT[A/G]GCCCCGAGCAGCATGAAGATTAAGGTGGTTGCACCGCCTGAAAGGAAGTACAGTGTGTGG 88 CmaMAB089 7 7244740 GCATGTGGTGGTGCTCACACTCTGTTCTTAACAGGTCTTAAACTTCTCTTCTTTCGAATT[T/C]GCAGCCTATATGAGATTGTTTATGGGATTTGTAGTGCTATGTATCTGTATCTATTTGAGG 89 CmaMAB090 8 134122 TTCGCCGCCGTTCCTCCGCCCTACCGCATTGAATGGGTTCGAGCTTCTTCACCACCGCTG[T/C]ATGCCCATTGGCCAGAACAGCCCTGTAGCATTTCCCATTTGAGTTCGAACTCAAAGCCAG 90 CmaMAB091 8 249703 TATCGTAAAGGAAATTTTAATCACTCATTTTTGTTTTTCCTTACTTGACTCAAATCCAAA[A/G]TGTCGCTGGTTGTGCTACTTTCAGGAGATCGAGGTTCTGACATCTTCGTTGAAGAGGTTA 91 CmaMAB092 8 1691166 ATTCTGAATTTCCCGAATGAGTACCAAAGTGGAAACCCCAATTCAGAGGCGGCGTTTGGT[A/G]GTTCTACGGCAGCGCCGTCTTCATCGACGGCGTTTTCTGGGAATTATTCCGGCAGCAATG 92 CmaMAB093 8 1984175 ATTAGATTTGCTCTTCCGAAGCCATTCATCGCCCCTATGGGCTTCTCTATCTCTGGCGGA[A/C]AATTCAGCCACCTCTTCAGTTGTTGACTCGAAGACTGGAATGTCATTTCCGTCAGTAATC 93 CmaMAB094 8 5427594 TGAATCAAACACAACGTTCGAAATTCAGATAAAAACCTGACCATATGAACTTTTTATTTG[A/T]CCACAGATCTTTCTGATTGGACTTCTCCGTCATTACCTGATAGTGATTTCTTCAACCATTG 94 CmaMAB095 8 6284315 TCTGTAAAGTCTGAGAATAGTTGTATGAATTCAATTTTTATTGCCTTGCACTTGGTTCAT[G/C]TGGTGCTCATCTGTTTATCTTGAATTATGACAATAAATTCCTCTTATCTGCTATTTCCTT 95 CmaMAB096 8 6813096 CCATTTAGTTGCTGCATTCCTGTCTCTGAAAGGCATTGGCGGGCTACTCTTTGTTTCTGG[A/G]AGTCAGATTGGAGCTTATCTTCTGGTGGAATTTTCTTTGCCTCTTTTTGATTACGCCTTT 96 CmaMAB097 9 243445 CACTTCAAAATAACCCAAATACACCAACAAACCTGAAAACAGTTTTAAAATTTGACGAAAA[A/G]GACGACGACCCAGTTGCTGAATTCTGTGGAGGCTAACCTGAAATCCCAAAGCCCCTGAAT 97 CmaMAB098 9 282970 CACATACGAAAGGCAATCGGCAGCCACGAACCTTGCAGGCTACTGGAAATACGATTGCCC[A/G]GTGATGTCGATGTCTCTGTCGCCAGAGGATGGCGGGGGCGCGTGCTTCGGCTTCATTCCC 98 CmaMAB099 9 400414 TTGATGATGCCATCAACGATGGGGTCGATGTATTATCCTTATCTCTCGGTACGCCCTACT[A/T]CTACAAACCGGAATTGGCGACCGACCCCATTGCCATCGGAGCCTTTCATGCAGTTGAGAA 99 CmaMAB100 9 2341391 TAGAGTTCATACTGCAGCTTCCCCTTTCCATCAACATGGCATCAGCAGGCACCGAACGGT[T/C]GGATTTTACATGAACCCCTGTATGGAGCAATCCAGGAGGTGAGGTGAATTCCAGTTCTTC 100 CmaMAB101 9 8118792 AGTCTGTCCATTGGCATTTCCATCCTAATATACGATCGCATAATCATTCCGATTGCAAGA[T/C]GTTTCACCGGGAAACCTTCTGGAATTACAATGCTACAGAGAATCGGATTTGGGATGATAT 101 CmaMAB102 10 629 TATTTGTATAAAGTCCGTGAATTGAACCAGTTCTCTATGGCTATATTATATTATCACAGG[G/C]GACAATTTCTTTTAGAGTCCTGACTCTGGTCTGCTTTGTTCTTAACTTGTTTGTCATAGA 102 CmaMAB103 10 7111 TCTATGACAAACAAGTTAAGAACAAAGCAGACCAGAGTCAGGACTCTAAAAGAAATTGTC[G/C]CCTGTGATAATATAATATAGCCATAGAGAACTGGTTCAATTCACGGACTTTATACAAATA 103 CmaMAB104 10 905057 GGGTCCACCACCACTCCTGGATATCTGACGGAGTTTGGCCATTTACAATTACGCCCCTTC[T/G]TCGTGTGGACACGCAATCAGCGGGGCCCACTTTCCTTTTACCCGCCCATCTCCAATTGGA 104 CmaMAB105 10 1053388 AGCCATTTTGTTTTAATATAGTTAGTGGAATTGGATGAGAACTCGCAGCCGTCAACAAAC[A/G]CTTCCCCAATGGGTAACAAAACCAGAGAATCACCAAAACTGGAAAACCATTAGCGCCCAT 105 CmaMAB106 10 1139391 TTCCAAAACGAAGGGTCATTTTCAACCTCCTTATATCCATAGGTGCTCTCTCCGATACTC[T/G]CGCACGATCACCGGCACGCTTGTAGGTGGGATCTACAGTTCAGAACAATAAGAAATAATT 106 CmaMAB107 10 1564009 TGCATTTCTATAGGGTTTAGCTCGTCTTCAAATTGTGTCAACTAGTGAAGAAACATGATT[T/C]CATGTTTTCTTGAATGGTTTTGAATACACCGTTTTTTCTTCGTAAGTTGCTTGTGCATTG 107 CmaMAB108 10 2388484 GTACAGTATATTTTGAATGATAAGATTTCGACTGCACATATATTTCAGTACAATTTTAGT[A/G]TAATAAGGCACATGTTCTTGAATGAGTGATAAAGATCACTTAGCTGCGTCTTCTGCATTT 108 CmaMAB109 10 2532615 TTTAACCATCTTAATCAAAAACCGCCATGTGTTTAGCTCTTAAAATGATTGATTCAAAAC[A/G]TACCAGAGTTTGAATGGAAGCAGAGATGAGAATGGTATAATATCGTCCAAGGAAAGAATC 109 CmaMAB110 10 2896039 AGCCTCCAAGCTTCTCTGCGGCTTCTATTTCTGATAGTAAGTTGCATTTGTTCAAGAACA[T/C]AGTTGCAGAAAATATTTGTTCGAGACTTCTGTAATTTTCACGTGAAGAATTTCTGACTTG 110 CmaMAB111 10 3118958 TTTCCCTTTGTTAGCGTTGAAGGCCTTCTAGATTGTGCTGTATATTGATCTGATGATATG[T/C]ACCCAAGTTTGCATCTGGGGCTCAAGCTGCCATGGCTAAGATGTTTGTATGAAATTTTGC 111 CmaMAB112 10 5118226 GTGGTTTTGGTTGAGCTTTGGGTGGTGGTGTGTCTATTGGAGTTGTTAGGGCACTTTCTT[T/C]TTGCGTCGAATTTCCAGAACGAGAGCTCGACGGCGTGGCTAGCGAGGACGAAGATATGAC 112 CmaMAB113 10 5653457 ACTCAATCCTCTTGGAACACTTCCATGGGTGTCGTTTTCCTCTCTCATACTCGTTCCTTC[G/C]AGAACACTTCTATCGATTGATGTTCTAAAGAACTCCATCAATTGCAATGGTGACTCTTTG 113 CmaMAB114 11 4379 ATTTCAAACAAAACAAAACAAACACCAAAGACAATTCAATGGGAAACAGTACATAGCAAA[A/G]GAAGTGGAAAAGAAGAAAAAGAGGAAAGAAATTGGAAATTTTGATAAACATTTGGATCTT 114 CmaMAB115 11 956302 CGGGGGAACTGGTGACACACAGAAACTAGAAGAGACACATTATGCAGCATTTAAATCTTA[A/G]TTAGATTGTATCTTGCCTGATTCATTGATCGTAGCTTTCAAGTACTTGCAAGTATTTGTTG 115 CmaMAB116 11 1146745 TGACAAAGCAGGAACTTCTTGTGAGGAGTGCTTTGAGGAGAACTGTGACACACAAAATGG[T/C]TATGTACCTGCATGCTCAAAAGTAATGTAGCAGCAAGCGACATTGAAACAACTGAAGAA 116 CmaMAB117 11 1200232 TCGAGTAACATTAGAACGAAAAAAACTGCTTCCAGATGTGAAACTTGAAGGTAATCAACA[G/C]CCATTTTCTTCCACCAAATTGAAGTGTTTCCGCAGCTAATTACTTATGGATGGAGTGGAA 117 CmaMAB118 11 1387566 CCCTATGCTATCCGACGGTGCAAAACCTCGCATCACGAACCCCGCGCGCCATTTCTCTAC[A/G]GACCCAAAGCGCCGATTCGCGGGATTTTGAAGGAAGACGCGTGGTGACAGCAGAGCAGCG 118 CmaMAB119 11 1456246 TTTAGCTGAAAAATAATTGTACTCCCTCTATAAATAAGTAACTCAATAGGCACTCTGACT[A/C]ATATAATCGGATTCTTGGTCATGGCCTTTTTTCAATCACTAATTTTCATTTTTTGGGCAA 119 CmaMAB120 11 1483544 GCCATTTCCTACATTTGGAAGGTAAATTTTAGACAAATAATAATCACCCAGAAGCAACAT[A/C]GAGACTATAAAAGGCTAAGAGTTTTGACAATATGCAAGGAAGGATCACCGACAATGGCA 120 CmaMAB121 11 1505535 TCCAAGTTACTGACGCAAGTTTCGGAGTCATCCGGTAGAAGCGGCAGGTCATACGACGGC[A/G]CTGTGTGGCCAGCAGCTGCGCCGGGTCGTCCAGCTCTCTGCAATGCGGTAGCGGAGTAAG 121 CmaMAB122 11 1915427 GTCGGACTTCGTTGATGGCATTGCGAAAGAGCAGCACAAGCAGAAGAAAAACAAAGATAG[G/C]GTTGGTGGGGAAGAAGTAGTGGAGGAGGCTGTGGCTGATGAGGATGAAATTGAGATGATG 122 CmaMAB123 11 1915505 AGTGGAGGAGGCTGTGGCTGATGAGGATGAAATTGAGATGATGAAGAAATTAGGTATTCC[G/C]GTGGGTTTTGATTCGACGAAGGGCAAGCCCGTCCCTGGTGCTGATGTAAGTGGCGTTAGG 123 CmaMAB124 11 2169202 TCGTAAACCACATTGGACAGCATATTTCAGAACAAATGACTTTTACAAAGTCACCTTTTG[T/C]TGATGATGTTCCGAATGCCAAGAAATGCTTAATGAAATTACACGTTACTTGCTGAGTGA 124 CmaMAB125 11 2668340 CCCATATTTCTTCGAACCAATCTCGAGCAGCTCGTTGTAACTTCCCGGAAGCAGAACCAA[T/C]TTCCCACCAACTTCGCCAATCTCTGGACAGCTGATAATCACCCTAGCAGGGTTGGTTCCT 125 CmaMAB126 11 2847775 TTCCACCTTTTTTTCCTACTTTAAATACCACTTGCGCCTTCCACCTGCCGCTCTCCCAAAT[T/C]CTTCACTGAGAGAGCCATGGAGAGACTTGAAGGGTGTCCCTGAAATTTACCAAGATTCTT 126 CmaMAB127 11 2879467 CGGCGAGGGCAAAGCGGGCGGAGAAGGAGCGGCAAAGAATATCGGGCTCTTCGGCAGGCGT[T/G]GCCTTGGTCGAAATTCTGTGAATAAGAGAAAGGATCATAAGCAATGAAGTTCCCTGAGGA 127 CmaMAB128 11 3056785 CACCAGCAGCTGCTATCGGTTCCTTTTTCTCTCCATATTTAGAATCCCAATTGCCTTTTC[A/G]GTTTCAGAGAGACAGAGAAATGGCTGGAGGAGGATTTGCAGATGGGGCGCCATTGAAAAG 128 CmaMAB129 11 3088780 AAAAAGGTCAAGCAGAGCAGAGACTGACAAAAAGAAAGGCTACATGTCATTGGCTCTATA[A/T]CAAATCATTAACTTAAATGTGCCCAAATGTTCGCCAGATTTTCTTCATGACATTCCCTAC 129 CmaMAB130 11 3237091 CTTGGCACTTACATGTGCTCCAAAGATGGCATCAACATTGTTCAATACTCCAGTCTCTAA[A/C]ATTTTCTTCGCTCCCCCACCTCCTTCCTCAGCTGGTTGAAAAACAAGAACTAGTGTTCCC 130 CmaMAB131 11 4330008 AGTTACAAAGGAACTGTCACAATAACATTCCAAATTCGTAAGGTACGATGATATTCCGCG[A/G]TCAATTGTAACCTCATTCAGCACGAGTATAGGTTCTTCAGTTTCGTATTCGTTTCTTGCT 131 CmaMAB132 11 4487577 TGTCCAGACACTTCAATTTCAAGGAAGAGCGGCGGGGCAGCAGAGGAAAATGAATCAAAA[A/T]TTTCAAACAAGGAAGACAAGATTAACATAAAAAAGGAAGGAAATATCCCTTCTGTTCAAT 132 CmaMAB133 11 6163595 CCGTTTGCACCGAGCTAAGCCGAGGGACCGTCTGCACCTATGTGTGACATGTGGCAGCAG[A/C]AAAACGAGCCACGTCGTTCGATAGCGTTGGGTCGAGGTACGGGTCACAATCCAATTTGGG 133 CmaMAB134 11 9542588 AAGAAAACCGGAAGAAACATACCCCTGAAAGATTAAGCTGCTTTAGCCAATTGCATAGCC[T/G]CATTACTACACCATCCACTTGGAAATTTTGAGGTATAGATCTTGAACCAGATTCATCTGT 134 CmaMAB135 11 9852403 GCAGGTGTGAAGCGAATTCCTCAAAAGCCACCGGTTCGACCGCCTATTCTTTCCCCTGCT[A/G]CCCCGTTTTCACCGTTGAAAACAATGGAACTTACTCCCGCTGCTCGGAAAAAGAAACAGA 135 CmaMAB136 11 10733918 TAACACAACAGGTGATAGATAGATAGGTCATCATTCTAGTAGAAGGCACCAGAAAAAAGT[A/T]ATTACCTGTAAATTCCTCCAAGACAATGGGAAGAACATCCTGAAATGGAGATTTCACTTC 136 CmaMAB137 11 10766251 AATACTCAAACCACGTGTTACTCTCATTTTTTAAATAAAGGTCGGGAATTTATGTATGGA[T/G]GATGAAATCACAGCAATGACCCTTAGGTTACCCATTGTCCTGGACCTGTTGTCTTCTTCC 137 CmaMAB138 11 10833318 GTTGGCTAGATTGCCAAGCTCAATAGGAATGCTTTCTCCAAAGGTGTTGCTTCTTAAGTT[T/G]AAGTAGAAGAGTTTGGAAAGATCAGCTATTTGTTTTGGTATTGGCCCACTTAGATTGTTG 138 CmaMAB139 11 11548910 AGGAATGCAGATTTGAAAGAGAAGCATGTCAGCTGGAATCTCTGTGAAGGGGTCCGATAC[T/C]CTGCCACAAAAATGGTCAAGAAAATAAAAACATTGCCAAAGAATTCCAAGTTCAAAGTTA 139 CmaMAB140 11 11924019 ACCACGACAAATATAGCTACAAAGGAACTCACTGAATCAGAAAGAAACAATCAAAATCG[G/C]ATTCGAGGAACTCGAGAAACTCGAGAAACGGATTGGGAACTTTCGCTAGTGTAGCTGCAA 140 CmaMAB141 11 12288503 CAGTACATCGACGCCCTCGAACCGCAGAGCTCGCACGCCACTGGCTCGCCTCCCCCGCCC[A/G]CCGCCGTCTTCTTGTCGGTTCTCCGCCGCTGCTCGACGGCGAGTTGGGGAGGTTCTTCTT 141 CmaMAB142 11 12334123 GAACCGTGCGTTGGCCTAGTGGTTAATAAACGTCGTGTAAATGATAAGGGACTAGAGAGA[A/G]CGAGTTTAAGTCATGGTGGTCGCTGCTTATATCTACAATTTAATGTTCTACAAGTTACCT 142 CmaMAB143 11 12452797 GGACAACCATCACTTTCGTCTGGAGGTGCATATATGATGTATGACAGGGAAAGTGGAAGA[G/C]CGCCGCACCATCCGCCTCAACAACCGCACTTCAACCAAAGTGGATATCCTCCAGCCAATG 143 CmaMAB144 11 12608099 TGGTCGTCGGGTGACATCGTACACAAGCAAAGCACCTACGGCTCCTCGGTAGTAAGCACT[A/G]GTTATCGCTCGGTACCTACACGGAAAGAGCTGATCTGAATTGCCAATAAAATAGCTGCGA 144 CmaMAB145 12 168122 GCTCCGGGAGCCGAGCCGGCGTCGGTTTTATTAGCACCGGCCTCGGGAGTTTCAGCAGGT[A/T]CGGCAGCGGAGGCGAAGCCGACAACGGCGAAGAGGACGAGAGCAACAACAACGACAAATT 145 CmaMAB146 12 336114 GTTGAGTCAGATCTGTGAATGTTGTTGGTGTTTCTGTCGTCGTTCTGTACCTTTTCACAGT[T/C]GATCCCTGATTTCACGCCAACGCCAACGTCACACACACACACACACACCACAGAGAAGAGACT 146 CmaMAB147 12 630209 TATGTATCAGCCATATCCTGCATCGTCACCTGCATCCATATCCCCAATGCCCAACAATCC[A/C]ATCAGTCCAAATTCCAATTCCATTTTCAGTTCCACAAATCCAAATCCCAGTTACCTCGGC 147 CmaMAB148 12 1203093 AAGGTAGCTACGGTAGCCGGGGCTGTACATGCAGCTCTCGGCGAACTTCACGAGATCCTT[T/C]GGTCGAGGAAGACGAGAAGCCAGACCTGCAACGTAATAGCATATGATACAAATGTTACTA 148 CmaMAB149 12 1621291 GGCGAGTTCACAAAGCTAGGTCTCATACTTCTTCCATTGACTTCATCATGCTTTATCTTC[A/T]TCTCTTCTCTGTTCTTTTGGTTTGCTCGTTATGCCTTCTCTGTTTCTATCTTCAAGCGTT 149 CmaMAB150 12 1803864 GTACGTCGCCAGTGCCTTCGCTTCGCATTGTACCTAAACAAGAAGCCAAAATGCAGCGTT[A/T]GATTTAAATCGAAAAGCATTGAAATGATAACATGGAAGAAACCAGAACGGAAGAGTAAA 150 CmaMAB151 12 3265959 CCCATTTGCATTGTTGTTGTACACACTTTCGTCCATAAATTGAGCTCCAAATTTGCTCAA[A/T]TTCTCAATTGGGTTGATATGGTCAGATGATGCCATGTTGTTGTTGTTGTTATAGTAGCTT 151 CmaMAB152 12 5793362 ACGTCTGCAGCATCCAATTCTTCATACAGTGCATAATTGATCCTAAATTGAATAGAAACA[A/G]ACAATCAGCAAATATTCACAAAATCTCCATCCTTACCAAATTAGAGATGATAAACATAGG 152 CmaMAB153 12 7425851 TTCATTTCAGTGGTTAGGATTTATGCTTTCCAAATAAACACTGATACATAAATTTGGTTA[A/G]CAGACATGAAATCCCTGTGGCATAGCAGCAAAAGCAAGTGATATTGCAAAGAAGAACTAT 153 CmaMAB154 12 7681837 CACCCAATGATCCTCCCGTAACTTTTTTTTTGGTCATCCCCAACCAAACAACTGATAAAC[T/G]GAACGAAAGAATGAATTCCATTTTAATTGCAGCCAAACCAACAACGAACAATCTCAAGTA 154 CmaMAB155 12 8037076 AATTGCAGCTCAGTTACCTGGAAGAACAGACAACGACATAAAGAACTACTGGAACACGAG[A/G]TTGAAGAAGAAACTGCTCGGAACTCCCAAACATGTCAGCTCGAGAAAGACTGCACAGACA 155 CmaMAB156 13 4444540 CGTTCTTCAGTAAAATCACCAACCAGCTTCCACATCTCTGTTTTCGACACCTGTCTAAAA[A/G]TTGCTTGCTTTGCCAGATCTTCAATACATTTCCCACCACTACATTTTTGCTAATTTGTAA 156 CmaMAB157 13 5101242 AACCAAAGCTCAGACTCCGTTGCTTTCTTCAGTAACAAAGGCCTTTAGCAATTAGAGTGA[T/C]CAATATCTCAGCCCCCAGTTGTCCAGTAGGCTAATCTTCTGCTGCTTTTACCAACAATGT 157 CmaMAB158 13 5235699 CCCCAACACTTCAGCAGGAGCTCTTGATAGTTCCTCTGGTGTCAATGAGATTGAGTCGTC[A/G]AGAAAATGAATTTCACCAGCCAACCGATCAGGTGATCTCACATCGAGTCTTGCAAGGTTT 158 CmaMAB159 13 6115552 TCTACCAGAAGAAGGAAAGTGAAGGTGCAAACGAGCAGCCGTATATAGAAATTCGAGAAT[G/C]GGAAATAAGCACACAAACAACACACAATTTCAGTCGCTCCATTCACTTAACTAATCACCAA 159 CmaMAB160 13 7510971 TTTGTTCAGCTTCATTGTAATGAAGCTCAACCTGACCACTGGTATTATACCTTCACTCAA[T/C]GTATCTGCTGGTCTATTAGGTTTCTTCTTTGTCAAGTCATGGACGAAATTTTTGGAGAGA 160 CmaMAB161 14 228042 GCTGGTGTAGGTGGTGAAAATTAGATTGCAGCAGCAGAAAGGACTGAGTCCTGATCTCCT[T/C]AAGTACAAGGGTCCAGTGCACTGTGCTCGAATGATTATTCGAGAAGAAGGCCTTCTAGGG 161 CmaMAB162 14 2509371 TCAAGATTTTAACGGAAGTTTCTGAGATTAAAGAAGTTCTATGTTGAAGTTAACTGGATT[T/C]CTAATTTCTTCTCCTTGAATCATATAGGTTCTTCGTGATCTTCTGGAGTTCAAAAGCGAT 162 CmaMAB163 14 3058992 CAGTAAGAAAGTTATCAGAAGGCCGTACGCATTTCCAAACAATAGTCCTACATAAACAAG[A/T]AAAACGAGAAAAATTAAAGCATACCTGTGGGTTGTTTCGAAGGAACTCAAGGGATCCAAG 163 CmaMAB164 14 3190380 TGGAGACGCAGCCCATGTACCACGCGGGGTTCGGGGCTCCGTGTGTGGTTTGTGTGGTCT[T/G]CTGGAAGTGCGCGGCCATGTGGTGGTGTGTGACGGATGCGAGCGGGGGTTTCACCTGGCC 164 CmaMAB165 14 4062455 AAACCACCAAGAATCTGGAAGTTGAGCTAGAACAAGAACAAGCTGCTCTAGAAAACGATG[A/T]ATCCATCAACTTTGGAAAATTGGCATCTAAACCTTCCAAAGTTGAAGCAGAACAAGATAT 165 CmaMAB166 14 4536346 ATCACTTGACAAGGCTTTGAACTTCGCCGCCGTCAATGGCTTGCCGGAAACTGTCAAGGT[T/G]TACGGAGGATACGATCAGATTCTCGACGACCCTTGTGTGGATGCCGTGTACATGCCACTG 166 CmaMAB167 14 5315189 CTATAAATCGAATCGATTCCATCTCATTTCCTCAGGCAGCAATTTCCAAAGCGAAGGCGT[A/T]TTTCGGTGATCCTTCTCGAGTTCTTCACCGGATAACGCCGCACAAATCCAGATCCAAAAC 167 CmaMAB168 14 5315263 TCTCGAGTTCTTCACCGGATAACGCCGCACAAATCCAGATCCAAAACCGAAAACAAAAAA[A/T]AACCTAATAAATCGCAATGGAGATGAAGAAGATCGCCTGCGCCGTCCTCTTCGCTGCCGC 168 CmaMAB169 14 7889662 GCATGCATGAAGAAGCAGCAAAAAGGCCACTGTTTTTCAATAAAATAATTGCCGAACGTG[T/C]ACTAAAGAAAGAGAGCTTTGCATTTTGAATGAGAAAACCTGCTGAAGAAGTGAACTTTGC 169 CmaMAB170 14 10213004 AAGATGAGTCCTCTGTTGAAGTCGTCTTGGGTCACTTGTGAAGCCAAAGCTTCGGCTGAT[T/C]GTAATGAACAAGTACATTAGTAACGATCGAAACCCGAGACTTAAACATAGCAATGGACTC 170 CmaMAB171 14 10925609 CTTTGTTTGTATATTAGGTTTGTATTTTCTTGTTGGTTTAATATCCGAAAAGTTGAGTGG[T/C]GTGACCCCTCTTGTATCCAGTTTAGCGTTCTTGAAGGTGTCTGGTTTTGTGATATGTGGG 171 CmaMAB172 14 11556897 GCCTTTTCATATCTGCTTGATGCTCTGGCATACTTACCAGCTTTGAACAATACGTTTCCC[T/C]CTTCCTTCTTTCTCCCTGCTGCTTCTATTTTCTCTTCCGCTGTTTTCAGATCGAAAGATT 172 CmaMAB173 14 11824513 AATTAAGCAGCTAGAAGGAAAAAAGAACCCACAGCAACATAAGAGAACAAAATGCTCTCA[T/G]ATTAGATCCATCGGCAACAATGAAATAAACATTATATCGCCTTGAACCAATCAAGATGGG 173 CmaMAB174 14 12266275 TTGCGGAGGAGGACGCCATCGGTGGTGGAGACAGCAAGCTTCGACTCTCCACAGCCCATT[A/G]TTGAGGCAAAATTGATGGATGAAGTGGCGGAGGAAGGAGAAATTCGAAGAAAATAGAATT 174 CmaMAB175 14 12756617 TTCTTTCAATACCATTTTCGGGCCACCGTGAATGGGTCGGGCACCGCCGCTGTTGGTGG[A/C]GGAAAGACGGTCGGGCGTTGGCTGAAGGAGCGCAAGGAGAGGAGGAAAGAGGAAAACAGA 175 CmaMAB176 14 13223193 AAATCCGCGCAGATCTCCTCTCCGGCGAAGAATTTCTTGTCGCCAAGGCATTGTTCTTAG[T/G]AATTCGATCGTCTCTGCCCTAGCTTTCTCCCTTTCTTCACTGCTGCGGGAACCGATTAAT 176 CmaMAB177 14 13688275 ATCTGTTGCAGCTTATAAGCTTTCTCAATTGGGAGCGAACTGGCAATGGCGGGAACTTGA[A/T]TCCACATCATCGTGCCTGTGGGAGTGCATTAGCATAGGGGTACAATTAGGGTTCTTGGGA 177 CmaMAB178 14 13692014 GCATTTGGTACCAAGTGAGCAGCTTAACCCCTATATACACTGTATCCTTACATTCTTGCA[A/C]TGCCTTTGAGTACTTATAAGCTAATTTCCGGTTATGTCAGCAATATTACACACCTACAGC 178 CmaMAB179 14 13853821 TTTTCTTCATCACTTTCCCGCTTAAACCTTATCCCAAAACCTTCACTTCCTTACGCGCTT[A/C]ATACTTTGAAATCACTGAACTTCCACAAGCTACTACGAATCAATGGGATTCGACCAGGTT 179 CmaMAB180 14 14681518 ATGAGGGCATTTACAAGAACGTCTGGTTCGGGCATGCCATGCAGCTCCATTTAAGCTCTA[T/C]AGTTGAGTCGAGCACGAACTCACCATCTGTTGCGGATTTATATTGAATTTGTCTTAAAGG 180 CmaMAB181 15 303606 GGATGAACTTTTATTGCAATCAATCACAGAAGAACTCAAGGCTGCAAAGAAGGATTTGGC[T/C]GCAATACGGGATGAAGGCTTTCAGTTCATGACATCGATGGATGCTGTACGAAGGGAACTG 181 CmaMAB182 15 303730 CGTGTCAGGGAAGAGAATGCTAATTTGAAGAAACCTGATGAAAAAACTGATTTAGTTGTT[A/C]AAAAACTAAACTCAAAGCTGCTTAGAGCAAAAACCAAATTGGAGGCTGTATCTTCAGCTG 182 CmaMAB183 15 826428 AAAGAGAAAACTCACAGCAAAAGCAACGATTGCTGAAGATAAAGCGAGAAATCCATACTT[G/C]GCCATGCCTTCAGAGAGGCCAACAAAGGTGTTCGCAACACCGAACATAATCCTCCAGAGC 183 CmaMAB184 15 1080846 TCTCTCATATGACATTGGAATTTGAAAACCACCAACCTTGTTTATGTATTTGAAAAGTTTT[T/C]TTTTCTTCAGTTGAAAACTGCCAAGAGTCTCGAATACTGTAATAGTGACTTTTGCTGCCG 184 CmaMAB185 15 1231300 ATTAGGATTTCTGCGTGACGTTTTGATATCTAGTAGCTCGACGGCTTATAATTAGTGTTT[T/C]TAGGATCGCAGCTCAAGGGGTCTTCTGTTAAGTGTTGCTTAAAATTGCTTATGCTTCTTG 185 CmaMAB186 15 1387753 TTGCCACATGCTAGTGAATTTTTTCCAGGACCCTTTTGATGGATTTGCAGCAATGAAACA[A/G]ACAGACGGATAGGTTCAGTAGCTCAGGGAATAGAGGCCAAGGAGTTCCGTTCGTAAACTC 186 CmaMAB187 15 2521766 CGACTTCACGAATTGAGGACTTCGATTGCAGCGACGATTGACTTCGCCGCGAGAATGAGC[A/G]GTGATCCAGTTCGGTACTCGCAGAGTTCTCATTAGGATTTGGAGTATCCATGGCGAAGAA 187 CmaMAB188 15 2662836 TACCAGTTTCAGAGACAAGTACAGCATGGGCCACGCTCGGGGATCAGGCGTGGTGGAAAT[A/T]CACAACCTGTACATCAGCAGCAGGTAAACTGAATTACGTTGTTAAGATCATCAATCTACT 188 CmaMAB189 15 2898973 TGGAAGTCTAGAATCACGACTGTCAATTCTATCTTGTTGCCGAATATTTTCATTCATTCC[A/T]GAAAGATCCCTGGATGAATGAGCTGTGCCATAGCTTCCCGGCCAAGCAGCAGCATCAGAT 189 CmaMAB190 15 3480990 AGTTGACACCCGCCGTAGATATCGATGCGGCGGCAACGGCGGACCTGGTGATGCTGGTTC[G/C]GGTGGTGGAGGGGGAAACCAAGAGATTTTTGCTGGAGAAAAATTTCTTCAGCTTGAAGTA 190 CmaMAB191 15 3523933 ATTAGGAGGCTGCAAAATGGTGGCAGAAGAAGCAGCCATGGAACAAGAGAAGCTCGCATC[T/G]CGGAATCCAAGGACGATTCCGATCACAGAGACTCAGTTCCTATCGTGGAAGCGGCAGAAG 191 CmaMAB192 15 3982228 GGCGGTGGCCGGGACGAGGAATCGGATTGATGATGCGGCTGAGAAGTGCGAGGATTGCGG[G/C]AAGCGGCGGCGGAGGAAGGTCGCGATCTTAGGGCTTGAAATCGGCGATCGAGATCGGAAT 192 CmaMAB193 15 5119413 GTCATTGCATGCCAAGTATAGCTGATCTTGTTCTTGTCTAGCATTTTATTGCAAATATGA[A/C]GCCTAGTGATAAACTCCTTTGCACTTTTGGAAAGCTAGCACATTGATATCTGCCGTAGTT 193 CmaMAB194 16 585086 GAGTCAGAAGAAGAAGATTCTGATGTAATGGTCATTGAACCAGAATTGAATTATATACTT[T/C]CTTCAGTGTTAACAACCTTGGGAAGGGCACCAGATATTCAGCGTGGAATAACAAGAATCT 194 CmaMAB195 16 1797865 GGCAGGGAGATTAGCTGAAGCAAGTAAACTTGTAGATGAAATGCCAATGCCAGCAGGACC[A/G]AGCATTTGGGGTTCGTTATTAGCTGCCTGCCGAAAATACCGCAATCTAGAAATGGCAGAA 195 CmaMAB196 16 1976655 TTCACAAGGAGAGGTTAAAAAACTAATTGGCAGGTTTCCTGGTTCCTTCAAAGAAACCTC[A/G]GAGTCGTCGATGCTACCGGGCCAGAAGTTCTCAAATCACAGTATTGTAGGCTCACATGAC 196 CmaMAB197 16 2834393 GATATATTAAAAATAAGTTTGACCATAAAAGATGGTGCAGAGGAGCAGCAGAGGGGTGTG[T/C]AGCAGAGGGGTGTGTATGCTGAGAACCAAGGGTTGTTCACTGAGCCCATGAACGTAGCTT 197 CmaMAB198 16 2834555 CTGCCCGCCAAACTGCCCGACAGGTGTACTCTTCTGGATATTGGTGAAGGACCACCATTA[A/T]ATTAACCCCTTTCTCACTCTTCTCTGCCCGCCAAGCTGCCCGACACACGTGACGACTCGA 198 CmaMAB199 16 3059938 CTGATTCCAGAGCTTCTCTAGGACGAGTCGAAACAAGTGCCATGGGGATCTTGTAATGCA[A/T]CAGGACATTCACAAACTCCTTTGATCCAGCTCGTAGTCTGTAAATTCCACCCTGCAATGC 199 CmaMAB200 16 3193799 TTACACAGCTTTGCCAAGGTCATAAACACAAGCTCAGGGAGAGATGAAACTTACTTCTTC[T/G]TTAGAGACCGCGCTTCGAGAAGATTGATATGTTCATTCCAAGAAATCCAGACTTCTTGC 200 CmaMAB201 16 7003738 GATCTCAAAGCTTTAAAAAGATTTGTGGTTCGTAAGATATGCATGAAAGCAGCAAAAAGT[G/C]GGAGCGAACCTGACAAATATCATCAACTTTGTTGCTCCAAGAGCTTCTTTGACATGTTGC 201 CmaMAB202 16 8655455 TCCCGATCTGTTCCTTGATCCTAATCACCAAATCGGTACATTCTGTTACTCGCAATTTCT[T/G]CCGATTGAGAGTTTCGACTCTGTTCTCGCTCCGCCACCGTCGCCGCCGCCGTCTGAATAT 202 CmaMAB203 16 9142930 GCAGCGAATCAAGCAGCCTTCTCTGGTGAAGGAGAAGCAAATACGGTACAGAAAATTCGC[T/C]ACCGCAATCTCGAATATGGATAGCCGATGGCCGGCGAGACGGTTAGAATACGCGGCGGAA 203 CmaMAB204 17 65947 CTGTCTTTGGTCGTAATAATGTTGCAGCTTTAGTCCAATTGCTTACTGCAACCTCTCTGC[G/C]TATTCGAGAGAAGACAATCACCATAATTTGCATGCTAGTTGAATCGGGAAGTTGTGAAAA 204 CmaMAB205 17 309846 AAGGAAGCGTTCGAAATATGCAAAAAATGGAGGGTAATCAAGAGTCCATTGACTGGTCTC[A/G]TCGAAGTACCATTGAGAAAGAGGGAGAGTGGGTCAGAGCGAGCCAATGACGATGGACC 205 CmaMAB206 17 625067 GGTTGTGGATCACTTTCATCAGGAATTGAGGTACTCATACTAGCACCTTCATCTGACCCA[G/C]TTACAGGCACGGGCGCACGAGAACGTAAAGAACGATTTCTTGAACCAGATCTCCTACCTC 206 CmaMAB207 17 5410180 TATGATCAGCTCTCTCTTTCGACCAATGTTGAGTTGGCAAAGAAGCCGTGGTCACACCCA[T/C]GCTCGCTTCATTACCAAAATTAAGAGCATTGTAACTCATCAGTTTCGCGCCTCCGTCGCT 207 CmaMAB208 17 6289237 TGATCGTGAAGGACGAGATGTGGAACGGTTTCGCCGCCATTACTATGGCAATGGGGAGCT[A/G]AAGCCAGCTGCAAAAGGAAGCAGAGGGGCAGAGTTAGTAGAAGGCCTTGAAATTAAAGAA 208 CmaMAB209 17 6375024 TATGTCTTTAAGCTTACAGACAGACTCATGTGTGATATTGAGCTCTGCCAGTAGTTCGTA[T/G]ATGTAAGGTCCAGGCTTAGTAAATCCTAATTCCTTGGACTGAATCGGGATCTCAGATATT 209 CmaMAB210 17 6882331 GATTGGTGGCCAAGATTTGTGATTTGGGGTTGGCCAGAAGAACCAGAGAGACAAGGCATG[A/G]CAATTGTTTTGGAGGGACGCTGTCGTACATGGCGCCGGAGACATTGATTCACCGCGTGCA 210 CmaMAB211 17 7228535 CCGTCACGTAGCCGCCGTACTGCGATAACGAAACCCTAGGCGGTTGTCCTGGCAGCCGTT[T/C]GATTTTGTCCTGCTGTTTCCGTCCGGCCTGATCGTCAATCTCGACCGTCGGCAAAACCTC 211 CmaMAB212 17 7689072 ATATTCGTGAGAAAGAAGTGGAAGATTTATTTTACAAGGTATTTTTTCTTAATTTTGTTT[A/G]CGCATGACGTTTTGGCGCCTGTGTGGAGCGCTTAAACCATGTAATTGTCTGTCATTTTGG 212 CmaMAB213 17 7904628 CACGATGATTAGTTTCCGGTCCAATGAAATCGCAATTCTAAACAAGAAGCATATGGCCTA[T/C]AATAGCTCAACGTATAATGGAAAGCTCAGACTAAATGACATGTTCTTGCAGCAAAATGGA 213 CmaMAB214 17 9119655 GGAACTCGGAACCTAGAAATCGTTTGTAGTAAAATAGATGATAATGAGCAATTAGGAATT[A/T]CCTGAAATGGAGGACAGACATCTTGGATTCATGTGGCAGAAGTCCACACGCAGTAATTGG 214 CmaMAB215 18 1727363 TCTCTTTTTCTTTGCCCTGCTGCACGATCTTTTTCTGATGTTCGAAACGACATTTTAAAA[A/G]ACAAATGGGAGAAGGAAAAACAGGCTAGTTTATCCATTACGAGTGAGCTTTATTCTCATA 215 CmaMAB216 18 2177624 CTTGATCCGGACCAAGTATGTCTTTTCAATAACTTGCAGCTTATGCATTCTCTAATGTGG[T/C]TGATTGCTTGAAATTCAGATCCAACGACGGCGACCAGATCTACGATTGATTATCTCTTCT 216 CmaMAB217 18 5979718 ACAGAACTCGGAAGAGACGGTGGACGTTAGCCATGAAAGACTAAAAAGATAAAGCCAAAC[T/C]GACAAAACACCGCTGTGCCGCCGCGTCGACCTCCGGCACAGCCGCGAATCAGCAGAGAGA 217 CmaMAB218 18 6588923 CAATCCTTCCATTGTTCTTCATGTCCTAACAGCATGTAACTTGGATCCGAAAGACCTTGC[A/G]AAGTTAGAGGCAAGTTCTTACGTTTTCGCTTGTTCGGGTTTACCAGCATTGTTGTGACTA 218 CmaMAB240 18 8013879 TTTCTCCTTCTCTTTCCTTGATATTTCCGCCCAATCCGTCGCCGTTCAGACTATGGTTCA[A/C]TTTTTTCGCACGTGCTATGGCCGGGAAAGTTCCCGGAATCTGCAGCCGTTCGCGGCATCC 240 CmaMAB220 18 8332005 AGAGAGATGCAAAACATCAATCCAGAAACAGGGGAACGATGCCAAAAGGATGAAAGCGAG[A/G]AACGAATACAACACTTCAATCCCCTTTCGGCAATTGGTACTATTAGACTACAGAAGCTTG 220 CmaMAB221 18 8568604 GTTCACCTTCTCTATTATCTTATGATGATAAAGACAACTGAATTCATCTTGATTCTTAAA[T/C]TGATATTTTGGATAATTGTCTTTCCCTCCCCAACAGCTTGAGGATTTAGGTTTCGACAGA 221 CmaMAB222 18 8653031 GCTCAGGTTAGTTTTCTGGTCTCTGAAAACCACCTTGAAAGTTCATCCGATAATTGTTGG[A/G]TCATGGAGTGCTGCTTATTTATTATTCAATTGTTATACATTGTAAAGCTATATATGAAAA 222 CmaMAB223 18 8781639 CCTGCATCCATGGAAGCGGCAACCAGACCTTCAATTCTTCTCACACTCGGCCGCATCCAT[A/C]TATGAGTGGTCTTCATTAGGTCAACCATCAAAAACTTCCGTCGTTTTGTTCTTCCTTT 223 CmaMAB224 18 9297432 GTTTCATTTCATGAATCTTCCACCGCCAATGGCTCTTTTGCCAACTGCCCAACCGTTGGG[T/C]TCCGGGATCGGTGGCAGTGGCGGCGGTCCAGCCACCGACACCCACTTGGGGATGTTAGCA 224 CmaMAB225 18 10242477 TTAAGTGATTGCTTATAGCATGCATAACTGAAGTATCTCTGGGTTATTCACAGGCTGCTA[T/G]TTTGAGAATCGCTGAGCAATATATACAAGCCTTCTGCAATATTGCCAAAGAGGTGACGTC 225 CmaMAB226 19 175180 GTAAAAGAAATTTGATGAGAACTAGAGAAGATTATCATACCTGGTGATGGCAGGGGAGAA[G/C]CTGATGCTGCAAGATATGATCAAGACCAACGTTAAAACTCATAAAATCTAAGAAATTGTT 226 CmaMAB227 19 6832521 GATGTTGAATCCAAAAGGGGAAGAAAAAGGTGCGTTTTGGACCAAAAATTGAGCTGTTTT[A/G]TCGGTCAAAAACAGGAACAGCAGGTTATGATTCAACTGTCATCCTAAGATGTCTTGTCTG 227 CmaMAB228 19 7268654 ACCACATCGGTGTCTTCCAATATAACATCAAGGCATTGAATCTACGCAATCAACACAAAG[A/G]AAAAACCCTAGTTCATTTCATATCCCAAAAATCACAATAAAAAATGCAGTGATGAACAAA 228 CmaMAB229 19 7705932 GGATCGGGCAATAAGAGGTATAGATTGAAGGGAGGTTTCGAAGAAGGAAGAGGAGGCAG[A/G]GATGGGGGAAGAAAGAACTCGCTTGAAGAGAAGCCATGCTAATTCGGGGCTTTTGGAATT 229 CmaMAB230 20 204472 TCTAGGCACATTACCCGATTTCCCCTGTTTCCAGGCAGTTGAATTGTCCTCAATAGTCGC[T/C]GAGACCAACTTCGCGACCTTCTTTGGGAATGACCCATCAAAATCTGCAGCATCAACGACC 230 CmaMAB231 20 792329 AAATTCCCGACACCCAATTCTTACTTCTTTCAACACCAACCTTCCATCTTCCCTGTACGA[T/C]ATGAAGCAAACCCCACCCTTTCCACTCCTTCCCATGAAAGACAAAGGCGGAGGCAAAGCT 231 CmaMAB232 20 1671948 TTCTCACCTTTAGAATAGCCACCTTTCATACAATCAAATGCTTCGCTCATAAAGTTCGAG[T/G]TCTTTTTATTGCATAGTATCTGGCACTCTAACAAAGATTTTCCATCTTCCAGCAGTATAT 232 CmaMAB233 20 2187664 GATTTCTATTTTCCCAAGTGGGGAAAGAACAAGTAATACCCATTGAGAAAAGACTTGAAA[A/C]CAAGTCTCAGTTTGCTACATTACGTATGCACAGGCTTCTTAACTAAAGATCAAAATAAAG 233 CmaMAB234 20 2339533 CCAACCCAGAAAATCCACTGAAGAACACAAGAAACAAACAAAAACCCATTAATTATTTGA[T/C]GATTACAGAGGAAAGAAGGTTTGAATTAATGAAATTGATGGATTACTTGATCATCCCAGG 234 CmaMAB235 20 2481036 TGATTCTGGTCAAGATGAGTTCGATTACGACTCGACTCCGGTACCGGAGGATGTCAAAGA[A/G]GGACATTTTGCAGTTGTGGCAGTTGATGCTGAGGAGCCGAAACGGTTTGCTGTTCCGTTG 235 CmaMAB236 20 3258105 GTACGCCATTGTTAAGATTCATTGTTTCCATGTACCATTCACTGTCTTTGTCCCAAAGAT[G/C]CTTGTAATCGTTGAGGAAATGAGTAGAGAATTTATCTTTCAAAGGGTCAAAGAAATCTGT 236 CmaMAB237 20 3682728 TCGATGGATGGGACATTCTGCTCCAAAGCTCGGATTTCCTTGTCTTTATGTACTCACCTT[T/C]TACCATAGAAGCCGCCATTGCGGAATTGTGGTCTACCGAAGCTGCCTATTGGAGTCTTAC 237 CmaMAB238 20 3801765 GCGCTTGGCTTGGCTACGGAGGGCGGCAGGACCGACTACGACGTTGCCGCAGTTGACGGT[A/G]GCGGAGGTGCGGTCGAGATAGTTGGCGTAGGCGAGGAGGAGGAAGGTGATGGAGGTGGCG 238 CmaMAB239 20 3843173 TGGGTAAGAATAGGATATCTGTACTTATTATGTGAAGATAGCTGCTCTTCTCACTTTCTA[A/G]AATCACTTTCTTTACTTTCTCTTACTTAGGCTCGGTTTCTTATCGCTCTTCGGCGCATCC 239 CmaMAB240 20 4138777 AATTTACGGCAACAAGAAAAGAAGGATCTCCGATACCTGCGCTGCAGCCGTCGACACCTC[T/C]GTCGATTGACCTCGTCGAAAACCTCCAACGGCCGCCGACGCCTCCATCAATTGGCCTCTT 240

The single nucleotide polymorphism (SNP) refers to insertion, deletion, or substitution of one nucleotide in DNA. The term 'polymorphism' refers to an arrangement in the sequence of a gene that varies within a population. Polymorphisms consist of different “alleles”. "Allele" means a gene corresponding to an allele located at a region corresponding to each other in a pair of homologous chromosomes, i.e., at a homologous locus, and refers to two or more of a gene occupying the same chromosomallocus. It means one of the above alternative forms. The placement of such polymorphisms can be identified by their position in the gene and the different amino acids or bases found therein. These amino acid variations are the result of two different alleles, two possible variant bases, C and T. Because the genotype consists of two different distinct alleles, any of several possible variants can be observed in any one individual (e.g., CC (denoted A), CT (denoted H) or TT (denoted by B)). Individual polymorphisms are also known to those skilled in the art.

In the present invention, the single nucleotide polymorphism may be located at the 61st nucleotide of each nucleotide sequence in the nucleotide sequences of SEQ ID NOs: 1 to 240.

The 61st base is the 61st base in the nucleotide sequence of any one of SEQ ID NOs: 1 to 240. The nucleotide sequences of SEQ ID NOs: 1 to 240 include single nucleotide polymorphisms and flanking sequences present on the chromosomes of western amber, respectively. In SEQ ID NOs: 1 to 240, each of the 61st nucleotides is a single nucleotide polymorphism present on the chromosome of western amber, and those associated with traits useful for breeding are selected. Analysis of the allelic type of the single nucleotide polymorphism may be useful for selecting western pumpkins having a desired trait by comparing the base difference between parents and progeny. When used for backcrossing, generation of backcrosses by comparing the genotyping results of single nucleotide polymorphisms of the recurrent parent and the backcross progeny for all or part of the 240 single nucleotide polymorphisms, and selecting backcross progeny with high similarity to the recurrent parent It can reduce the number of tremors.

The preparation may include a polynucleotide consisting of a nucleotide sequence identical to or complementary to a continuous nucleotide including a nucleotide corresponding to the selected single nucleotide polymorphism. The continuous base may consist of 5 to 121 bases. For example, the agent is a polynucleotide consisting of all or a part of the base of any one of the alleles located at the 61st position in any one of the nucleotide sequences of SEQ ID NOs: 1 to 240, and a polynucleotide consisting of a sequence complementary thereto, or a polynucleotide consisting of a sequence complementary thereto may be nucleotides. In the nucleotide sequence of each SEQ ID NO: A single nucleotide polymorphism is located at the 61st base, and a flanking sequence of 60 bp each in the upstream and downstream directions from the 61st base is linked to the SNP. One single nucleotide polymorphism is located in the nucleotide sequence of each SEQ ID NO. The length of the continuous base may vary depending on the SNP genotype determination method.

According to one embodiment, the agent may be for identifying a single nucleotide polymorphism located at the 61st base of SEQ ID NO: corresponding to the polynucleotide to be detected or amplified. The agent may be a primer comprising all or a part of a base of any one of the single nucleotide polymorphic alleles corresponding to the 61st base of any one of SEQ ID NOs and a side sequence continuous thereto.

According to one embodiment, the agent may be any one of a probe, a primer, and a combination thereof.

The term “probe” refers to a polynucleotide that specifically binds to a particular target sequence. The probe may be complementary to or capable of specific binding to a polynucleotide including a single nucleotide polymorphism site. The probe may have a difference in binding strength depending on the difference in bases of single nucleotide polymorphisms. The probe may be labeled to confirm the presence or absence of a specific nucleic acid.

The term "primer" refers to a single-stranded polynucleotide capable of serving as an initiation point in the polymerization of nucleotides by a polymerase. The primer may be prepared using a method known to those skilled in the art according to the selected SNP analysis method. The primer sequence may be the same as or complementary to some continuous bases among the base sequences of SEQ ID NOs: 1 to 240. The length of the primer may vary depending on various factors such as temperature and the use of the primer. For example, in the case of a primer for HRM (High Resolution Melting) analysis, it may be a primer pair capable of generating an amplification product of a size (eg, 50 to 500 bp size) that is easy for HRM analysis, including the base of the polymorphic position. .

The primer may be a primer for Fluidigm SNP analysis. Using the Fluidigm system, two alleles, SNPs, can be detected rapidly in large quantities. Fluidime system uses LSP (Locus Specific Primer), ASP (Allele Specific Primer) 1, and ASP 2 as primers and optionally uses STA (Specific Target Amplification Primer). The ASP1 and ASP2 may be prepared corresponding to the allele of the single nucleotide polymorphism. The 5' end or 3' end of ASP1 and ASP2 may have the same or complementary base to the allele of the single nucleotide polymorphism to be analyzed, respectively. Fluidime SNP analysis can use FAM and HEX as probes. FAM and HEM may be connected to any one of ASP1 and ASP2, respectively. For example, ASP1 and ASP2 are specifically hybridized to two alleles of the SNP, and the result is amplified, and the genotype of the SNP can be confirmed by concurrent amplification of the FAM and HEX sequences linked to ASP1 and ASP2. . A primer for the Fluidime system can be ordered and manufactured by Fluidime.

Another aspect of the present invention provides a kit for identifying trait differences between varieties of western pumpkin, comprising the composition.

In one embodiment of the present invention, the kit may be a PCR kit, a microarray, or a kit for Fluidime SNP analysis.

The PCR kit may include a primer set capable of detecting the single nucleotide polymorphism. The PCR kit may further include a DNA polymerase, a dNTP mixture, and a PCR buffer solution. The DNA polymerase may be, for example, a klenow fragment of E. coli DNA polymerase I, a thermostable DNA polymerase, or a bacteriophage T7 polymerase. The PCR buffer may contain KCl, Tris-HCl and MgCl2. In addition, the PCR kit may include components necessary for the identification of the amplification product. The PCR kit may include a guide. The guide may be a printout explaining how to use the kit, for example, how to prepare a PCR buffer solution, suggested reaction conditions, and the like.

The microarray may be a polynucleotide capable of detecting the single nucleotide polymorphism, for example, a probe immobilized at a high density in a divided region on the surface of a substrate.

The Fluidime SNP analysis kit may include primers for the Fluidime system, and specifically may include STA, LSP, and ASP 1&2 primers. The kit for Fluidime SNP analysis may further include a DNA polymerase, a dNTP mixture, and a PCR buffer solution. DNA polymerase and PCR buffer solution may be understood with reference to the description of the PCR kit.

Another aspect of the present invention is a) isolating a polynucleotide from a repeat parent western amber sample, and determining the genotype of all single nucleotide polymorphisms present at the 61st position of each nucleotide sequence shown in SEQ ID NOs: 1 to 240 to do; b) isolating polynucleotides from backcross progeny Western pumpkin samples, and determining the genotype of the single nucleotide polymorphism in step a); c) comparing the single nucleotide polymorphism genotype of the repeat parent determined in step a) with the single nucleotide long polymorphism genotype of the backcross progeny determined in step b); and d) selecting a backcross progeny with a high ratio of matching repeat parent and single nucleotide polymorphism genotype from among the backcross progeny.

The method for selecting backcross progeny of western amber consists of isolating a polynucleotide from an introduced western squash sample, and comprising a single nucleotide polymorphism present at position 61 of any one of the nucleotide sequences shown in SEQ ID NOs: 1 to 240. determining the genotype of one or more single nucleotide polymorphisms selected from the group; The method may further include comparing the single nucleotide polymorphic genotype of the introduced parent with the single nucleotide polymorphic genotype of the backcross progeny.

The composition for identifying trait differences between varieties of Western pumpkin according to one embodiment can determine the difference in traits or trait-related genotypes by discriminating single nucleotide polymorphism genotype differences, and using molecular markers to determine backcrossing (MAB) or varieties etc. can be used.

When the composition for identifying trait differences between varieties of Western pumpkin according to one embodiment is used for Western pumpkin backcrossing, individuals having a similar SNP genotype to the recurrent parent can be effectively selected.

When determining the similarity of the SNP genotype of a western amber using the composition for identifying trait differences between varieties of western amber according to an embodiment, it may be associated with the similarity of the western amber trait, so the similarity of the western amber trait It can reduce the time and cost required for judgment or increase the accuracy of judgment.

1 is a genotype analysis result (group B and group C, some) for Western pumpkin F ₁ varieties (5 pieces) and parental lines.
2 is a genotype analysis result (group A and group D, some) of 40 various breeding materials used in Western pumpkin breeding.
3 is a genotype analysis result (group A, group B and group D, some) of 41 strains of various breeding materials used in Western pumpkin breeding.
4 is a genotyping result using Western amber SNP molecular marker (CmoMAB011).
5 is a physical map showing the location of the western pumpkin SNP 240 marker set for which efficacy was tested.
6 is a genetic phylogenetic tree analysis and classification results for western pumpkin core lines and breeding materials (total 80).
7 is a result of genetic phylogenetic analysis and classification for Western pumpkin F ₁ and parental lines (total 15).

Hereinafter, one or more specific examples will be described in more detail through examples. However, these examples are for illustrative purposes of one or more embodiments, and the scope of the present invention is not limited to these examples.

Example 1: Materials and Methods

1-1. test material

In order to select SNPs that can be used for various breeding combinations of western amber, 226 squash resources were collected and 40 core western squash strains with no overlapping characteristics in various traits were selected (group A and group B). .

As materials for the test of the marker set to be developed, 5 varieties of sweet pumpkin, large tree (Western) F ₁ , their parents, 10 lines, and the remaining 40 breeding lines were used (group A, group B, group C, group D). ).

1-2. GBS analysis of western amber core strains, NP search and selection between strains

High-purity gDNA was isolated by the CTAB method, and it was confirmed that the A260/280 value was 1.8 or higher and the concentration was 100ng/μL using Nanodrop to meet the standards.

A GBS library was constructed using the restriction enzyme Ape KI, and the library was subjected to nucleotide sequence analysis for each core line of Western amber using Illumina Nextseq 500 equipment.

SNP calling of the core line was performed using the SAM Tools program, and SNPs satisfying the selection conditions were selected through SNP filtering.

1-3. Developed in the form of a Fluidigm marker set, the strain and F _One applied to the breed

The selected SNP was developed as a custom primer (or probe) set for the Fluidigm ^® EP1 ^TM Genotyping System, and using this, it is possible to assay multiple molecular markers on multiple samples within a short time.

The designed SNP marker set was applied to 40 core strains out of 226 collected amber resources, and also to 5 F ₁₅ varieties and their parents, 10 strains, and 40 strains of various breeding materials used in Western pumpkin breeding. Genotyping was performed.

Based on the genotyping results of high-throughput marker sets, SNPs showing polymorphism between strains used in Western pumpkin breeding were additionally selected, and through this, Marker-assisted Backcross (MAB) Breeding is possible.

Genetic phylogenetic analysis was performed to analyze the genetic diversity of each lineage.

Example 2: Study Results

2-1. Selection and collection of Western pumpkin core strains

Young leaves (new shoots) of 226 resources including various characteristics such as fruit size, fruit type, skin color, leaf shape, presence or absence of marble pattern of leaves, and thinness, which are amber traits, were collected (groups A and B).

2-2. GBS Analysis and SNP Search of Western Amber Core Lines

To construct a GBS library by extracting high-purity gDNA, it was fragmented by read using Ape KI restriction enzyme. Different adapters were attached to both ends of the fragment and ligated with T4 DNA ligase.

The nucleotide sequence of the constructed GBS library was sequenced for each amber resource using Illumina Nextseq 500 equipment, and then the adapter sequence was removed through the trimmomatic program and trimming was performed. As a result, the average number of reads for each of the collected 192 resources (group A) and 34 resources (group B) was 1,765,076 and 2,523,739.

The read mapping was performed on the standard genome using the Bowtie2 program, and the average overall read mapping rate was 84.0% for group A and 81.8% for group B.

Using the VCFtools program, SNP calling was performed to search for genomic variation information between the standard genome and western resources (49) among the collected amber resources, and as a result, 7,478 final SNPs were searched.

The number of SNPs for each chromosome was calculated using the in-house script , and the ratio of home/hetero genotype for each sample was also calculated.

2-3. High-throughput custom SNP selection and marker set development

A total of 7,478 SNPs searched in groups A and B were selected for genotype results with a depth of 5x or higher, and those with different genotypes were classified as SNPs to select a total of 2,659 SNPs. SNPs with homozygosity of 0.96 or higher were selected. 2,128 were selected.

Among the selected SNPs, 341 SNPs with PIC (Polymorphism Information Content) values of about 0.35 to 0.5 were finally obtained in order to select SNPs suitable for tasks such as backcrossing group or purity test as much as possible.

A Fluidigm ^® SNP Type ^TM assay primer (or probe) set that performs PCR amplification based on flanking or amplicon sequence information, which is a 60 bp nucleotide sequence adjacent to the SNP position in both upstream and downstream directions, was prepared. Through the D3 assay design site, 335 were synthesized except for 6 that could not be designed.

2-4. Efficacy test by applying SNP marker set to Western pumpkin lines and varieties

The 335 Fluidigm ^® SNP Type ^TM assays designed were applied to various breeding lines and F ₁ varieties used in the development of Western pumpkin varieties to test their effectiveness.

The genotype analysis was analyzed for 40 core lines and 5 varieties of F ₁ , 10 lines that were their parents, and 40 lines of various breeding materials used in Western pumpkin breeding ( FIGS. 1 to 3 ).

As a result of the analysis, it was confirmed that 240 of the synthesized 335 Fluidigm marker sets functioned as molecular markers, except for 95, which exhibited a heterozygote genotype in most strains or were ambiguous to detect with a fluorescent probe (FIG. 4). A physical map was prepared for 240 SNP marker sets whose efficacy was tested (FIG. 5).

Information on the final set of 240 SNP markers tested for efficacy by genotyping is shown (Table 1).

It was confirmed that SNPs showing polymorphism between lines were selected with the developed 240 Western amber SNP marker sets, and individuals of the backcross generation similar to the genotype of the recurrent parent were selected early and used for generation-shortened breeding (Figs. 1 to 1). 3).

In order to analyze the genetic diversity of each line used in the analysis, a phylogenetic tree (Dendrogram) analysis was performed. Phylogenetic analysis was performed in Darwin6 software (http://darwin.cirad.fr/) using the neighbor-joining method. The structure of the phylogenetic tree was based on an unweighted neighbor-joining method, and bootstraps were determined to be 1,000 replicates.

As a result of genetic diversity analysis of a total of 80 western pumpkin core strains and breeding materials distributed from group A and group D, they were largely classified into 2 groups. All of the nurturing materials were included in the first group, and the core lines were all classified in the subgroup (FIG. 6).

Genetic diversity was analyzed using 240 Fluidigm marker sets developed for a total of 15 western pumpkin F ₁ and parental strains distributed from groups B and C. Both F ₁ and parental lines were genetically similar but classified ( FIG. 7 ).

The above results can be summarized as follows:

Forty western amber core lines with various traits (e.g., fruit morphology, fruit color, leaf plaque and thinness, etc.) were selected, DNA was extracted from young leaves, and a GBS library was constructed using ApeK I restriction enzyme. The nucleotide sequence was analyzed using Illumina Nextseq 500.

335 ^SNPs were selected based on criteria such as sequence depth, polymorphism information content (PIC), ^homozygosity , separation ratio, and SNP proximity, and 240 SNP primers (or probe) set was developed.

The developed marker set was applied to 40 core strains, and as a result of testing a total of 40 breeding strains, such as sweet pumpkin and rootstock (Western), and ₁₅ Western F1 and parent strains, which are used for breeding by breeding companies, It was confirmed that 240 primers (or probes) out of a total of 335 functioned as molecular markers.

Through these results, the set of 240 markers finally tested for efficacy showed polymorphism for each breeding line, so it can be used as a marker-assisted backcross (MAB).

So far, with respect to the present invention, the preferred embodiments have been looked at. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

<110> Republic of Korea (Management: Rural Development Administration) <120> SNP MARKER SET FOR IDENTIFYING CUCURBITA MAXIMA CULTIVARS AND METHOD FOR IDENTIFYING CUCURBITA MAXIMA CULTIVARS USING THE SAME <130> RDA-P200047 <160> 240 <170> PatentIn version 3.2 <210> 1 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 1 agaaagatgg acctgaagaa gggatattgt ctgatgttgc cttgcgtgct ccatcttgtg 60 yttcatcaaa gcataatgag aagctaaatc 213> CUCURBITA MAXIMA <400> 2 accatgttct aggcgtctcc tcaagaagaa aagacatacc catccatcaa aatcaagatc 60 yaagtcatgt tcttgagaaa atagccactg cccatcaata acaaactgga aaacccgaga 120 a 121 <210> 3 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 3 tagttgtact tagtttgtgt gccccttcta cagggaaatc agcatgtgca aatggctgga 60 ratgcttctc atgggagtca cgtggatgct tctgctaaag gtgaaacgta cggccagcag 120 c 121 <210> 4 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 4 gcgatcatgg agtcaaatgc tgaaaacccc acaagtttaa acctataatg aattacagtg 60 racaagtttc aaactgctct tcttttgcag caatggtgac ccccattttc gttcatgtca 120 a 121 <210> 5 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 5 cacaagagga caccagaaat taccaagttc aacttgtacg cacttacaca agaagagttt 60 wtagggaaag aaaatgggac actagccaaa acaaaactta ttttacctgg ggctgcaggg 120 a 121 <210> 6 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 6 catacacctc tcggctccat tccaagtgcc tcatgtcagc ttcaggggat tcccatgtaa 60 yatagtagc aatttttatg ggataccctg 212 <ctcag 7 > DNA <213> CUCURBITA MAXIMA <400> 7 ggagtgagtc gtcatctcct accccgatta ggcaaaaagg acccgttgct taggaatgtc 60 ratttattca tattattgca actttttctt caggggct cagggggct gtattttcaa 213 g Cag <121> TA gg t gagggct 213 cctttttcaa gtatttttcaa tcttggagat gatggcctct gatgaaggtg gctcttgacg 60 yagttccttg acaacagcag ctagagcctc cggattcact cccatgtcac aaagagcaat 120 a 121 <210> 9 <211> 121 <212> DNA <213> CUCUR BITA MAXIMA <400> 9 aatagctatg gttgcaacaa aagcagcaat tgaagcgaga ccaagattct tatacaaaac 60 yaacagggcc agtacaactt gaaaaaccac cagccaaaca tcatgcatat accaactgaa 120 c 121 <210> 10 <211> 120 <212> DNA <213> CUCURBITA MAXIMA <400> 10 gcatattatt gaactttcct gctaacctct aattttggac aacgattcag tccttgtcat 60 rcttattatt agtttttctc caggagttga tggtgggttt gatagcaatg aacctgaata 120 120 <210> 11 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 11 aagtcaagga agttcagaga acaatcaagg tatgatatac tatgaatcta catatgtgcg 60 rtttttgctg cctaaattca catataaact atactctagt acattgtctg aaactcgata 120 t 121 <210> 12 < 211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 12 ccatcccttt tatctgttgc atcaacacct aatacattta cagcagcgaa agagcaaaac 60 ycaaagaggc aaaatgattt cttttcattac aaagtt IMt 211 121 UC gt ctt cttttcattac aaagtt IMt 211 121 UC gt ctttata 13 400> 13 gacgggacat ggcattgtta gaagcggcaa gagggcgtcg gacgggggagg ggatgaaggg 60 ytgtcccccg gcagccaaat cgacatgatg attgggtcta atgctacaca ttccagagtc 120 t 121 <210> 14 <211 > 121 <212> DNA <213> CUCURBITA MAXIMA <400> 14 ctgtttgacg agaatgttgt gaagtgtttc tatccgactc cgtccgatga aacaagggag 60 sttttggttg tgttgcctgt cggaattggt 210ct 212 DNA 212 cggaattggt <400> TAgtt > 15 atgttttgag aaacaagcct tacagatatc atcattgttg ccacaatttt taaatgaaaa 60 mtatgatcga acaaacgaaa aaaacagcta tcttaatatg ttcacaagag actcagacat 120 c 121 <210> 16 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 16 tcggctgcga cggcaacggc ggcgacggaa aagcgaagag agattgacag aaggaagaaa 60 yaaccgaagg gggagggaga gaaagagaga gaggttttat agtgaaaatt agtgagtgtg 120 g 121 <210> 17 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 17 ggcaaacgcc ggtccagaca caaatggctc acaattctttc atcactacag tca caactag 60 ytggtaaactg <212> DNA <213> CUCURBITA MAXIMA <400> 18 aatctcatac acttccttaa cgaattgctg gcctcgcgcc acgtcgtgcg acggatggtc 60 rtcgttcgcc ggaaaaagat tgacgtcgaa gtaacgctgt tcaatcactg atttgg 120 g 121 <210> 19 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 19 cttccttatg gcaacagtct tacccaacat ttcacccttg taaacacaac catatcctcc 60 mttgcaatc ttgaagctt 211 cagagaagcc 21 212> DNA <213> CUCURBITA MAXIMA <400> 20 tgaagctttc agagaagcca catgtggctg tttgtagttc caacaatgaa aattcagcca 60 kctgagtttg attctccaca aatccgatcg attgttata atttgt cacaactcat ggaaggcact attgctgatt ccaagatcat gcccacacga 60 ytgtttgaac tctgagatca atgaattttc aatcgtccca ctttctggtg cagccacata 120 a 121 <210> 22 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 22 gttattcaac gcctccatta gtgtactcat catcggtggc agcaaatcct gaaacctgtc 60 rcgatcagca gagttcgaca agcactgtat gaagctgata acagcattga gggccgcaat 120 c 121 <210> 23 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 23 gtagagtagc agttttatca taaccaagta ggctgtggtt tgaattaaaa ttttcaggtc 60 stgttgatga agcattcctg catccat cta ggtaatctct accgattgca gctttttcat 120 c 121 <210> 24 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 24 aagaaactct ggaaaatcga tagttccatt tccatccgca tcgactttcgt aca tca ttgactttcgt gctt t ttg211 gacctc 60 acc > 121 <212> DNA <213> CUCURBITA MAXIMA <400> 25 aagcctttgg atttgatgag ggaatagcac cctcatgtgt taaatactga gaaacttctg 60 yatcatatgg attttcactt tgagataatc ccctattg CA211 121 121 TAaagact <400> > 26 cctacaaaaa caaatggctt ggttgacata tgcaaggaaa tatcagatct tacctgcgta 60 ktcgttgtcc aaagagttct catcgtcact ttctgccaat gtatctccac cagtgccctc 120 g 121 <210> 27 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 27 tgttggcgag gagggaactc cgggatacgt tggaccgagg ttaatattgt ttggtggtgc 60 yactgcgctc gagggcaact ctgctgcctc aggaactcca tcatcagctg gaagtgcggg 120 a 121 <210> 28 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 28 gcacttgccc accaacaaac tatgtgtatg tttgactcat aacttattct acacgttctt 60 rtaaaccaag caccagagtt taacggcgta tattatgttg tctgcatgat acagcaatga 120 t 121 <210> 29 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 29 gtcgcacatt cttacgctcc ccatcattgc agcaaggtgg agaggattgt ttcccctctc 60 rtctgtagtc tctagaactt tcttcttagt aattgtgtct ttggtggctt cgcttatgtg 120 t 121 <210> 30 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 30 gcttgtgcat gcttggtgcg gtggtgatgc gagcgtttt gctctgtgag gatttggggt 60 rttgcagctt gctggcttt tctt ttagatgctttt 211 121 UCTC 121 CTC 211 <212> <400> 31 ggcaatacct acgagaccaa ctaccggagc tgcgacgaat agccaacttc gtcgacctct 60 scctgaattg taactattac taacagcagg gtctatgttg ctcagctcct ctccttggct 120 g 121 <210> 32 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 32 aagaaagaga ccgaccgaga ggaggtggca gaaggggtgc agctgagagc aggtggtgcc 60 sttcaaccgc tctctcaccg attgcatgac cgccacgact cctcttcttc aaatcccaac 120 c 121 <210> 33 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 33 aatgagcttc atttcctcca agacgcacct catgt actcg aatgatcctg cctttccaag 60 yttgcgaaga atctccttgt agaacgacga actaggcacg aagttgggct gcttcgaagc 120 c 121 <210> 34 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 34 aacttctgtg gaacgatcag cagcattaga taacacattg gaacgaggga ttgataagtc 60 wccttgtcgt ccattaccta catgaaatat ccaattattt cctgtcaaat atacattcac 120 a 121 <210> 35 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 35 tgtcttgggc ccaacttccc ccttcaatag tttattgact atacgaatgt tggcgaacgt 60 kccacgtgcc ataatctctcgt cattccccacg gcgact 121 aat DNA 120 gcgacttta 212 t 213> CUCURBITA MAXIMA <400> 36 tctcacccaa atagacaaaa agttcacttt ttttcgttaa atcatattga atcttaaaag 60 wgcttactct gaaactgtca gtggaagacc catatccgta atttcccata tgccccacgg 120 c 121 <210> 37 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 37 cgtcagacga gtttttgggc ttgacggccc caaaattctc ctccaagaac ctttccattt 60 ytctgagtaa gttagtgaaa ataaacttag gtatccgcag ttatataaag aaaggaaaca 120 a 121 <210> 38 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 38 agatacac cg ctcatataac atagaccact gaaagccttg aagacacata aattatacct 60 sggcaagacc tcttccagtg accacgccaa tgctagccga gagatactgg cacaaaatag 120 c 121 <210> 39 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 39 ttgctgctca aaatcattga acaattggat cagatgttga gattctttta gaacagattc 60 ktctaatttt tcccaagtgc attcgtcgta aggaaggcca ctccatttta taaatgcttc 120 a 121 <210> 40 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 40 gaaactcact gctttcatcc cacaacactc tttctgagtc ggcattcctg cattgtcggc 60 yaaggtttca aattccgacc ttgttttcg 212c <400> 41 atga 121agt acagt tga > DNA <213> CUCURBITA MAXIMA <400> 41 taattttaaa taaataaaaa aaatatttaa gttgtttgat tagctgcagg tttgttgccc 60 wtgaaacgct aaggaagcat gaaattgaag tattaccaaa gattgatc cattctgaac aacaggaggc tggtcatgta gatcgtttaa 60 ygacgatgca ttggactttc ctctatgatg accactcagc ttgacctcga aaccccgtct 120 a 121 <210> 43 <211> 121 <212> DNA <213> CUCU RBITA MAXIMA <400> 43 ttgattgaaa ggttactttc tagaatcttt ctttgtcgtt ttatccgagg agttgcagaa 60 ktttcccctt ttttcatcat tatgtcgttt tatcttgaca tctaggttct tccacttgct 120 c 121 <210> 44 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 44 cttcaaagcc tctccggcta tggccatgag atccttggaa agcgaaattc cgactttgcc 60 ytctttgtcc tgctcctcga agacgcagcg gtaggccttg tcgtcggctc ctttgtgagt 120 g 121 <210> 45 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 45 gcagctagaa agcagttcct agagacagga taatggagag ccaaaaccag gtcactctca 60 saggtgccgc ttgaaggcac caccttcttc gcaactggta tcaaatttgg gagtctaaaa 120 t 121 <210> 46 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 46 tagaaaagaa gacctctcac agttgacatg ttacctgtat agttatcgca gctagaatca 60 rgctcaggat cacgttcaga atccataacc tcaacctcat tagcggattc <400> 47 cctgcgcacc caaatcagct gttgacgatg gagcctcaat gcatgtctgt agtttagtca 60 saatggaaag cagctctcat attatatata tgcacatcag aatctcgata gctcgaatat 120 t 121 <210> 48 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 48 acgcccacat caacgaatcg tggcccagcc cgaacaccag aaaattgcag ggacatcgag 60 ycttcaatac gtcgaatgag atactgatct cgtcaaacga 211 <400> 49 aatgtttgag acaattgaaa ggaagccaag cattgacgtc tatgatccaa agggtaaaac 60 rttagatgat attcagggag acatagattt gaaggacgtg agtttcagtt atccaaccag 120 g 121 <210> 50 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 50 ttatgagttg ctagcataac caaaagaact cttggctgga tgttggaatg aacatgaagc 60 waattgggaa tggttgctgc aagggggatt ctcaacagaa gttgtttagt agggtgcatt 120 t 121 <210> 51 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 51 taccggccag ttcttcaggt ttctgattgt tattcatacg gacgatcaaa tatgaataaa 60 yaagtctaaa tgggtttctg tcctgacata ttttatggtt tctgcagttc acagataagg 120 t 121 <210> 52 <211 > 121 <212> DNA <213> CUCURBITA MAXIMA <400> 52 gtgtctcttc tccaggctct tgtggccgaa aaacagattt tacagcattt gttgcacgta 60 katgctgccc acttaaactc agcttggtag aataagaacc aatggtttc atgcgctttg 120 c 121 <210> 53 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 53 aaacttctac tcgataacct gttgtttttt cttacatcat tggtatgctt agcagatttt tgg tag 210 macttctaaaa 210 atttgcttg 211 g acc 212> DNA <213> CUCURBITA MAXIMA <400> 54 taaaattcgg caaaaaattc ctcactcatt acatgtctcg ctgcgtctct ctaatttcca 60 stactttttg aatctgttttt gttt 121 TAtgtg MAXIMA <400> c ttatgt 213 TAttga <ttag tt 121 acctttg cagggaagga gtcatgaaga gtcaagcaaa aaacgacagg gcgggatgga gaagtcatga 60 rggttaaaaa gttgaaggga gaacggcatg gtgtatggtt gagctcatgc ctttgctgca 120 a 121 <210> 56 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 56 atttctgagt acaagaagcc caaaagctcc tagtgatttg gttggctctg aatagcgcga 60 rgtcagagag gaggaggaag aagaagacga tgggaactct ggggaaagcc atttacaccg 120 t 121 <210> 57 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 57 ttgatcacct ccgacacacg catcggagga ctggatttgg tgccgaccat ttcttgaatg 60 rttagttaaa tgtgaggcag ctt tacctgg aacatcaata cctacaaatt ttaccggtat 120 a 121 <210> 58 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 58 aaataagaac cgcggattga cggttcggtc tgctccgttt gaaggttttgc ctg ggggatggc aggttt gaaggtttttgc ggtg ggtgagga 59g > 121 <212> DNA <213> CUCURBITA MAXIMA <400> 59 tttaaattgg tggaattgta caggacgaaa tcaaattggg aatcttcata ctacgagtgc 60 rgctgctgcg tctttcggtg ggttactgac gccgtat > 60 gattgaaatc caacaaatcc tagacttgtt gattgatgat ctgaggaggg aaggttatat 60 wcctaatacc actcttcttt agctagacta actttcattc atgctcattg ttttgttctg 120 t 121 <210> 61 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 61 aaaactgaac gaatatccac aaagaagaac ttttccttca accaaacgtt tgtttagaac 60 rtcaggcctt tggcagtctg aagggctgta cttcgatact gaagaatcta acagaacgtc 120 a 121 <210> 62 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 62 cggctgtatg aagagaagga taaaatgcac gcactgaacg gaatggaata ctcatttgc t 60 rtcgctgccg cttccttcag gacagcatac agtctgaaca agaccttgta tctttggtat 120 a 121 <210> 63 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 63 ggtcgataga gagacgggga agaatcgact tctagcctct gccctaggct ccatgctcgt 60 ygtccaccca caggtattcc tctacctcaa cctcgattcc atcattcttt tgagagtagt 120 a 121 <210> 64 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 64 agcttcgtgt tgcagaggtc ggaatgtttt gtttccaaaa ataacaacat tcgccaattc 60 raaatcatcc aaagtctgta actcttcata agtaagagtt ttgtgctcaa cccctgaagc 120 t 121 <210> 65 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 65 atttcgcagc ttgagtagga aagctaagat aatgttgggt taactcagtc agagatatga 60 kgtgggacat a aga ttggggatga cagatact 211 g <121> acagctt > DNA <213> CUCURBITA MAXIMA <400> 66 agatacttct gagatgaaag gggagttcga gcatattaga gaaaaagaga aggatttatc 60 raaggagaca agggaagaga gggaagagcg gttgcagcgt gagaagattc 121 gagaagattc 121 gagagattc 121 c gaatacgtgg gaaccgtggt ttcgatactt cactttctag 60 raatggtctc tattctggtg gaaggtattt tccaactggt aaccatatta ctcggtattt 120 g 121 <210> 68 <211> 121 <212> DNA <2 13> CUCURBITA MAXIMA <400> 68 tttcattgga gaaggttctt acggtcgggt ttattatgca acgttaaagt gcggtcaggc 60 ygctgcaata aagaaactgg ataccagttc ttcaccggag cccgacaacg attttgcagc 120 t 121 <210> 69 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 69 aagagaaatg cggtactcgg acttgaagaa gatcgaccgt ggggttcggc gtcattttca 60 ygacgacaca acggtgatag ttgtgtttct gggttcgagt ggttcaggag gaaggagccc 120 t 121 <210> 70 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 70 agattttccc ttttgattca gccgttctta tcttagccgt tcttctttca atcttcgata 60 ytgttctcaa aatctcagcc ttcgctgttt cccccatttt cctaaactcc tcagcttcag 120 a 121 <210 > 71 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 71 gttaagttac acttgtaact aatatactta cttttgttaa ctttatgcag ctcgcgggcc 60 rgaaacgaca gtcgattggg caacaaggat 210 a CUCURBITA MAXIMA <400> 72 ctggtccgaa gacacctcct cgtgcaaatt cctgtcaaag tgacaagtct acatctccac 60 wtgagatttc ttctgctgct gattgcagta acaacaatac tccacaagac gttagtccta 120 c 121 < 210> 73 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 73 cggtgtgcgg ccactttctt ttgttaaaaa ggaaacgtga gcagaaagca ggacatgaag 60 wagaaactaaaa agtgataaat gga <ggcag 213 cgaacaa att 210 cagtataaat 121 DNA > CUCURBITA MAXIMA <400> 74 gttgagagcc aagttgaaat gaagctgatt atcggtgagg ttgaattggg tgagtttagc 60 ktcggtgacg tggaatttga ctttgttggg acggaatatg agccataaga tcaatacggc 120 g 121 <210> 75 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 75 actgcatttg tctgatcatc tagcctttca tttaaccctg aagatttctt tttcaccaac 60 scatccgact tcattacttg ctcactaaca ttggcagccc tcgtcaggct ttctagagcc 120 t 121 <210> 76 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 76 gctcaaccga tgctgcaacc ccctctggta gctcgactcg ggccacgtat gtccctggac 60 mtcccacatt cttaactctt ctattgattg tcaccggagc attcacattt agatttggaa 120 t 121 <210> 77 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 77 tcttccctgc agtgcaaatc aagatcttcc ccttcacctt cttcggatta agcgttccct 60 ygccgcaaaaa tttcctgcaa tttaactcaatttc atcatcg aagcagcagt 120 t 121 <210> 78 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 78 agtggtagct gcagcgagta ttttgctgta acctcttcaa ttcaactaactc atgttgccttc aggattcattat gaactaact atgtttgcctt 60 yatattcagcc aggagcatattat 120cc t 121 <210> 79 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 79 taagggcacg ctaacctaat aaagcgtttc aatagagttc caggaatcta gttccataca 60 yaaaaaaggat gtcctcagaa ct 210 212 aaaaggat gtcctcagat c DNA <213> CUCURBITA MAXIMA <400> 80 tgttaaaatt catcagttca gtccagtttt tgaacaaaaa tgagaagaat caaggcaata 60 mgcaagaatt tttgtaccat cttagagaca caattggaga ttggaatggc ggctttggct 120 c 121 <210> 81 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 81 atatcatatg caaagcaaaa ccggttcgga tgcttaccga gaaattcgac agagtcggtg 60 scaacagagc tccaggcagc ccatatgaaa tttccaggag ctaccaagtt gggcttcata 120 a 121 <210> 82 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 82 accgctctta cattgccctt ggcagctatc aggtctgttc gtgcacatta gtgtggttgt 60 waagtatatt ggtgattgct ttcaacttga tttgatactc tgtcgtactc tttcagctag 120 t 121 <210> 83 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 83 ctcatccgcc cccacgcctc tccaagccgt caaattgctc gctctttatc tgtctgatcc 60 yagcagcaag gtttcattcg tggatttta tcct tag ctgattatgt tcgtttggaa 120 t 121 <210> 84 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 84 tgcctcactt tcttcatttt gaaagctatt 210 gcatgcagcagca ggttggtcttt ca ggcttggtattgt accata 211 cttgtgtattgt accata <213> CUCURBITA MAXIMA <400> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 85 caaagcaagc tttattataa tactgcttaa aaagtagcca ttactgcatc aaaataagca 60 saaaggcaaa gaacagatag agaggagatg aacacacttt tataag <211 c 86 gaaggagcgc cattgatgag agctcaagct tccaaatcca tggctgtgtg cttctctttt 60 ygcctttctt ctccattggc ttctttctgt tggggcagtc aatagctcca taacgtaaaa 120 a 121 <210> 87 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 87 tcggtccggt gtagggttgc tgagaagcaa ttaaagagaa gaagcaaaac agagaggcga 60 ygaagaaatg gaatttgaag gaacccattg gaaaataaga gagattttgg agatgggatt 120 c 121 <210> 88 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 88 cggtggttca accatgttcc ctggcattgc tgatagaatg agcaaggaga tcgcagcgtt 60 rgccccgagc agcatgaaga ttaaggtggt tgcaccgcct gaaaggaagt acagtgtgtg 120 g 121 <210> 89 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 89 gcatgtggtg gtgctcacac tctgttctta acaggtctta aacttctctt ctttcgaatt 60 ygcagcctat atgagattgt ttatgggatt tgtagtgcta tgtatctgta tctatttgag 120 g 121 <210> 90 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 90 ttcgccgccg ttcctccgcc ctaccgcatt gaatgggttc gagcttcttc accaccgctg 60 yatgcccatt ggccagaaca gccctgtagc atttcccatt tgagttcgaa ctcaaagcca 120 g 121 <210> 91 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 91 tatcgtaaag gaaattttaa tcactcattt ttgtttttcc ttacttgact caaatccaaa 60 rtgtcgctg tt gaggat 210 210 rtgtcgctg tt gagg 212 <400> t a > DNA <213> CUCURBITA MAXIMA <400> 92 attctgaatt tcccgaatga gtaccaaagt ggaaacccca attcagaggc ggcgtttggt 60 rgttctacgg cagcgcgccgtc ttcatcgacg 210 BIctt ttcatcgacg gcgtttttctg 93 ggaatt <120 g Cgg gccattcatc gcccctatgg gcttctctat ctctggcgga 60 maattcagcc acctcttcag ttgttgactc gaagactgga atgtcatttc cgtcagtaat 120 c 121 <210> 94 <211> 121 <212> DNA <2 13> CUCURBITA MAXIMA <400> 94 tgaatcaaac acaacgttcg aaattcagat aaaaacctga ccatatgaac tttttatttg 60 wccacagatc tttctgattg gacttctccg tcattacctg atagtgattc ttcaaccatt 120 g 121 <210> 95 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 95 tctgtaaagt ctgagaatag ttgtatgaat tcaattttta ttgccttgca cttggttcat 60 stggtgctca tctgtttatc ttgaattatg acaataaatt cctcttatct gctatttcct 120 t 121 <210> 96 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 96 ccatttagtt gctgcattcc tgtctctgaa aggcattggc gggctactct ttgtttctgg 60 ragtcagatt ggagcttatc ttctggtgga attttctttg cctctttttg attacgcctt 120 t 121 <210 > 97 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 97 cacttcaaaa tacccaaata caccaacaaa cctgaaaaca gttttaaaat ttgacgaaaa 60 rgacgacgac ccagttgctg agt 211 212 gaa ca ca acct 212 <121> CUCURBITA MAXIMA <400> 98 cacatacgaa aggcaatcgg cagccacgaa ccttgcaggc tactggaaat acgattgccc 60 rgtgatgtcg atgtctctgt cgccagagga tggcgggggc gcgtgcttcg gcttcattcc 120 210> 99 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 99 ttgatgatgc catcaacgat ggggtcgatg tattatcctt atctctcggt acgccctact 60 wctacaaacc ggaattggcg agaaccgaccgtcca 210 g > CUCURBITA MAXIMA <400> 100 tagagttcat actgcagctt cccctttcca tcaacatggc atcagcaggc accgaacggt 60 yggattttac atgaacccct gtatggagca atccaggagg tgaggtgaat tccagttctt 120 c 121 <210> 101 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 101 agtctgtcca ttggcatttc catcctaata tacgatcgca taatcattcc gattgcaaga 60 ygtttcaccg ggaaaccttc tggaattaca atgctacaga gaatcggatt tgggatgata 120 t 121 <210> 102 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 102 tatttgtata aagtccgtga attgaaccag ttctctatgg ctatattata ttatcacagg 60 sgacaatttc ttttagagtc ctgactctgg tctgctttgt tcttaacttg tttgtcatag 120 a 121 <210> 103 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 103 tctatgacaa acaagttaag aacaaagcag accagagtca ggactctaaa agaaattgtc 60 scctgtgata atataatata gccatagaga actggt tcaa ttcacggact ttatacaaat 120 a 121 <210> 104 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 104 gggtccacca ccactcctgg atatctgacg gagtttggcc atttacaatt acgcccctttc ggt 210 cctcatgc ggcaatt acgccttttc 121 <212> DNA <213> CUCURBITA MAXIMA <400> 105 agccattttg ttttaatata gttagtggaa ttggatgaga actcgcagcc gtcaacaaac 60 rcttccccaa tgggtaacaa tgggtaacaa aaccagagaa tcaccaaa <> 212 CURccca Accagagaa tcaccaa t 106 ttccaaaacg aagggtcatt ttcaacctcc ttatatccat aggtgctctc tccgatactc 60 kcgcacgatc accggcacgc ttgtaggtgg gatctacagt tcagaacaat aagaaataat 120 t 121 <210> 107 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 107 tgcatttcta tagggtttag ctcgtcttca aattgtgtca actagtgaag aaacatgatt 60 ycatgttttc ttgaatggtt ttgaatacac cgttttttct tcgtaagttg cttgtgcatt 120 g 121 <210> 108 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 108 gtacagtata ttttgaatga taagatttcg actgcacata tatttcagta caattttagt 60 rtaataaggc acatgttctt gaatgagtga taaagatcac ttagctgcgt cttctgcatt 120 t 121 <210> 109 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 109 tttaaccatc ttaatcaaaa accgccatgt gtttagctct taaaatgatt gattcaaaac 60 rtaccagagt ttgaatggaa gcagagatga gaatggtata atatcgtcca aggaaagaat 120 c 121 <210> 110 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 110 agcctccaag cttctctgcg gcttctattt ctgatagtaa gttgcatttg ttcaagaaca 60 yagttgcaga aaatattttgt tcgagacttc tcgagacttc tcgagacttc tgactt 111 gIMA121 DNA 210 act <111 gIMA121 <211> <400> 111 tttccctttg ttagcgttga aggccttcta gattgtgctg tatattgatc tgatgatatg 60 yacccaagtt tgcatctggg gctcaagctg ccatggctaa gatgtttgta tgaaattttg 120 c 121 <210> 112 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 112 gtggttttgg ttgagctttg ggtggtggtg tgtctattgg agttgttagg gcactttctt 60 yttgcgtcga atttccagaa cgagagctcg acggcgtggc tagcgaggac gaagatatga 120 c 121 <210> 113 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 113 actcaatcct cttggaacac ttccat gggt gtcgttttcc tctctcatac tcgttccttc 60 sagaacactt ctatcgattg atgttctaaa gaactccatc aattgcaatg gtgactcttt 120 g 121 <210> 114 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 114 atttcaaaca aaacaaaaca aacaccaaag acaattcaat gggaaacagt acatagcaaa 60 rgaagtggaa aagaagaaaa agaggaaaga aattggaaat tttgataaac atttggatct 120 t 121 <210> 115 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 115 cgggggaact ggtgacacac agaaactaga agagacacat tatgcagcat ttaaatctta 60 rttagatgta tcttgcctga ttcatttgat 212 gtagctt a <213> CUCURBITA MAXIMA <400> 116 tgacaaagca ggaacttctt gtgaggagtg ctttgaggag aactgtgaca cacaaaatgg 60 ytatgtaccc tgcatgctca aaagtaatgt agcagcaagc gacattgaaa caactgaaga 120 a 121 <210> 117 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 117 tcgagtaaca ttagaacgaa aaaaactgct tccagatgtg aaacttgaag gtaatcaaca 60 sccattttct tccaccaaat tgaagtgttt ccgcagctaa tacttatgg atggagtgga 120 a 121 <210> 118 <211> 121 <212> DNA <213> CUCURBITA MAXIM A <400> 118 ccctatgcta tccgacggtg caaaacctcg catcacgaac cccgcgcgcc atttctctac 60 rgacccaaag cgccgattcg cgggattttg aaggaagacg cgtggtgaca gcagagcagc 120 g 121 <210> 119 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 119 tttagctgaa aaataattgt actccctcta taaataagta actcaatagg cactctgact 60 matataatcg gattcttggt catggccttt tttcaatcac taattttcat tttttgggca 120 a 121 <210> 120 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 120 gccatttcct acatttggaa ggtaaatttt agacaaataa taatcaccca gaagcaacat 60 mgagactata aaaggctaag agtttgagac aatatgcaag gaaggatcac cgacaatggc 120 a 121 <210> 121 < 211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 121 tccaagttac tgacgcaagt ttcggagtca tccggtagaa gcggcaggtc atacgacggc 60 rctgtgtggc cagcagctgc gccgggtc> 210 212 MAXIMA <400> a 400> 122 gtcggacttc gttgatggca ttgcgaaaga gcagcacaag cagaagaaaa acaaagatag 60 sgttggtggg gaagaagtag tggaggaggc tgtggctgat gaggatgaaa ttgagatgat 120 g 121 <210> 123 < 211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 123 agtggaggag gctgtggctg atgaggatga aattgagatg atgaagaaat taggtattcc 60 sgtgggtttt gattcgacga agggcaagcc cgtccctggt gctgatg 400> 124 tcgtaaacca cattggacag catatttcag aacaaatgac ttttacaaag tcaccttttg 60 ytgatgatgg ttccgaatgc caagaaatgc ttaatgaaat tacacgttac ttgctgagtg 120 a 121 <210> 125 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 125 cccatatttc ttcgaaccaa tctcgagcag ctcgttgtaa cttcccggaa gcagaaccaa 60 yttcccacca acttcgccaa tctctggaca gctgataatc accctagcag ggttggttcc 120 t 121 <210> 126 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 126 ttccaccttt tttcctactt taaataccac ttgcgccttc cacctgccgc tctcccaaat 60 ycttcactga gagagccatg gagagacttg aagggtgtcc ctgaaattta ccaagattct 120 t 121 <210> 127 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 127 cggcgagggc aaagcgggcg gagaaggagc ggcaaagaat atcgggctct tcggcagcgt 60 kgccttggtc gaaattctgt gaataagaga aaggatcata agc aatgaag ttccctgagg 120 a 121 <210> 128 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 128 caccagcagc tgctatcggt tcctttttct ctccatattt agaggatcccaa ttgagatttttc ttg 210 agagga 120 g ctgg agagga 120 g ctgga 121 <400> <212> DNA <213> CUCURBITA MAXIMA <400> 129 aaaaaggtca agcagagcag agactgacaa aaagaaaggc tacatgtcat tggctctata 60 wcaaatcatt aacttaaatg tgcccaaatg ttc213 c UR121 BI210 130 TAIMA 121 c cttggcactt acatgtgctc caaagatggc atcaacattg ttcaatactc cagtctctaa 60 mattttcttc gctcccccac ctccttcctc agctggttga aaaacaagaa ctagtgttcc 120 c 121 <210> 131 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 131 agttacaaag gaactgtcac aataacattc caaattcgta aggtacgatg atattccgcg 60 rtcaattgta acctcattca gcacgagtat aggttcttca gtttcgtatt cgtttcttgc 120 t 121 <210> 132 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 132 tgtccagaca cttcaatttc aaggaagagc ggcggggcag cagaggaaaa tgaatcaaaa 60 wtttcaa aca aggaagacaa gattaacata aaaaaggaag gaaatatccc ttctgttcaa 120 t 121 <210> 133 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 133 ccgtttgcac cgagctaagc cgagggaccg tctgcaccta tgtgtgacat gtggcagcag 60 maaaacgagc cacgtcgttc gatagcgttg ggtcgaggta cgggtcacaa tccaatttgg 120 g 121 <210> 134 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 134 aagaaaaccg gaagaaacat acccctgaaa gattaagctg ctttagccaa ttgcatagcc 60 kcattactac accatccact tggaaatttt gaggtataga 121 TA 135 <400> <400> 135 gcaggtgtga agcgaattcc tcaaaagcca ccggttcgac cgcctattct ttcccctgct 60 rccccgtttt caccgttgaa aacaatggaa cttactcccg ctgctcggaa aaagaaacag 120 a 121 <210> 136 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 136 taacacaaca ggtgatagat agataggtca tcattctagt agaaggcacc agaaaaaagt 60 wattacctgt aaattcctcc aagacaatgg gaagaacatc ctgaaatgga gatttcactt 120 c 121 <210> 137 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 137 aatactcaaa ccacgtgtta ctctcatttt tta aataaag gtcgggaatt tatgtatgga 60 kgatgaaatc acagcaatga cccttaggtt acccattgtc ctggacctgt tgtcttcttc 120 c 121 <210> 138 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 138 gttggctaga ttgccaagct caataggaat gctttctcca aaggtgttgc ttcttaagtt 60 kaagtagaag agtttggaaa gatcagctat ttgttttggt attggcccac ttagattgtt 120 g 121 <210> 139 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 139 aggaatgcag atttgaaaga gaagcatgtc agctggaatc tctgtgaagg ggtccgatac 60 yctgccacaa aaatggagatcaa gaaa gtata 121a <400> 213> CUCURBITA MAXIMA <400> 140 accacgacaa atatagctac aaaggaactc actgaatcag aaagaaaaca atcaaaatcg 60 sattcgagga actcgagaaa ctcgagaaac ggattgggaa ctttcgctag tgtagctgca 120 a 121 <210> 141 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 141 cagtacatcg acgccctcga accgcagagc tcgcacgcca ctggctcgcc tcccccgccc 60 rccgccgtct tcttgtcggt tctccgccgc tgctcgacgg cgagttgggg aggttcttct 120 t 121 <210> 142 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 142 gaaccgtgcg ttggcctagt ggttaataaa cgtcgtgtaa atgataaggg actagagaga 60 rcgagtttaa gtcatggtgg tcgctgctta tatctacaat ttaatgttct acaagttacc 120 t 121 <210> 143 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 143 ggacaaccat cactttcgtc tggaggtgca tatatgatgt atgacaggga aagtggaaga 60 scgccgcacc atccgcctca acaaccgcac ttcaaccaaa gtggatatcc tccagccaat 120 g 121 <210> 144 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 144 tggtcgtcgg gtgacatcgt acacaagcaa agcacctacg gctcctcggt agtaagcact 60 rgttatc cc ac ataga 121g ct gatct 145 aga rgttatc 212> DNA <213> CUCURBITA MAXIMA <400> 145 gctccgggag ccgagccggc gtcggtttta ttagcaccgg cctcgggagt ttcagcaggt 60 wcggcagcgg aggcgaagcc gacaacggcg 146 aagagg400acga gagcaata atctgtgaat gtgttggtgt ttctgtcgtc gttctgtacc ttttcacagt 60 ygatccctga tttcacgcca acgccaacgt cacacacaca cacacacaca gagaagagac 120 t 121 <210> 147 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 147 tatgtatcag ccatatcctg catcgtcacc tgcatccata tccccaatgc ccaacaatcc 60 matcagtcca aattccaatt ccattttcag ttttttcag tttttacaaat ccaaatccca 213 c 121 UCUR210 TA 400 IM211 121 Ccaaatccca <212> aaggtagcta cggtagccgg ggctgtacat gcagctctcg gcgaacttca cgagatcctt 60 yggtcgagga agacgagaag ccagacctgc aacgtaatag catatgatac aaatgttact 120 a 121 <210> 149 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 149 ggcgagttca caaagctagg tctcatactt cttccattga cttcatcatg ctttatcttc 60 wtctcttctc tgttcttttg gtttgctcgt tatgccttct ctgtttctat cttcaagcgt 120 t 121 <210> 150 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 150 gtacgtcgcc agtgccttcg cttcgcattg tacctaaaca agaagccaaa atgcagcgtt 60 wgattttaaa tcgaaaat 211 ac 121 g gaag aaaat 211 ac 121 g <212 gagtaaaagca <400> > DNA <213> CUCURBITA MAXIMA <400> 151 cccatttgca ttgttgttgt acacactttc gtccataaat tgagctccaa atttgctcaa 60 wttctcaatt gggttgatat ggtcagatga tgccatgttg ttgttgtgt tatagtagct 120 t 121 <210> 152 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 152 acgtctgcag catccaattc ttcatacagt gcataattga tcctaaattg aatagaaaca 60 racaatcagc aaatattcac 211cc ata 121 153 at tagagatcagc aaatattcac aa <121> 212> DNA <213> CUCURBITA MAXIMA <400> 153 ttcatttcag tggttaggat ttatgctttc caaataaaca ctgatacata aatttggtta 60 rcagacatga aatccctgtg gcatagcagc aaaagcaagt gatattgca <at 213 aga <212>acca 120 tcctcccgta actttttttt tggtcatccc caaccaaaca actgataaac 60 kgaacgaaag aatgaattcc attttaattg cagccaaacc aacaacgaac aatctcaagt 120 a 121 <210> 155 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 155 aattgcagct cagttacctg gaagaacaga caacgacata aagaactact ggaacacgag 60 rttgaagaag aaactgctcg gaactcccaa acatgtcagc tcgagaaaga ctgcacagac 120 a 121 <210> 156 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 156 cgttcttcag taaaatcacc aaccagcttc cacatctctg ttttcgacac ctgtctaaaa 60 rttgcttgct ttgc cagatc ttcaatacat ttccccaccac tacatttttg ctaatttgta 120 a 121 <210> 157 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 157 aaccaaagct cagactccgt tgctttcttc agtaacaaag gcctttagca attagagtga 60 ycaatatctc agcccccagt tgtccagtag gctaatcttc tgctgctttt accaacaatg 120 t 121 <210> 158 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 158 ccccaacact tcagcaggag ctcttgatag ttcctctggt gtcaatgaga ttgagtcgtc 60 ragaaaatga atttcaccag ccaaccgatc aggtgatctc acatcgagtc ttgcaaggtt 120 t 121 <210> 159 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 159 tctaccagaa gaaggaaagt gaaggtgcaa acgagcagcc gtatatagaa attcgagaat 60 sggaaataag cacacaacaa cacacaattt cagtcgctcc attcacttaa ctaatcacca 120 a 121 <210> 160 <211 > 121 <212> DNA <213> CUCURBITA MAXIMA <400> 160 tttgttcagc ttcattgtaa tgaagctcaa cctgaccact ggtattatac cttcactcaa 60 ygtatctgct ggtctttttag gttagatcttttt tgg 212 212 agtcttttt tgg 212 agtcttctt tgg > 161 gctggtgtag gtggtgaaaa ttagattgca gcagcagaaa ggactgagtc ctgatctcct 60 yaagtacaag ggtccagtgc actgtgctcg aatgattatt cgagaagaag gccttctagg 120 g 121 <210> 162 <21 1> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 162 tcaagatttt aacggaagtt tctgagatta aagaagttct atgttgaagt taactggatt 60 yctaatttct tctccttgaa tcatataggt tcttcgtgat 213 212 tca121 CBIC TA t 400> 163 cagtaagaaa gttatcagaa ggccgtacgc atttccaaac aatagtccta cataaacaag 60 waaaacgaga aaaattaaag catacctgtg ggttgtttcg aaggaactca agggatccaa 120 g 121 <210> 164 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 164 tggagacgca gcccatgtac cacgcggggt tcggggctcc gtgtgtggtt tgtgtggtct 60 kctggaagtg cgcggccatg tggtggtgtg tgacggatgc gagcgggggt ttcacctggc 120 c 121 <210> 165 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 165 aaaccaccaa gaatctggaa gttgagctag aacaagaaca agctgctcta gaaaacgatg 60 watccatcaa ctttggaaaa ttggcatcta aaccttccaa agttgaagca gaacaagata 120 t 121 <210> 166 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 166 atcacttgac aaggctttga acttcgccgc cgtcaatggc ttgccggaaa ctgtcaaggt 60 ktacggagga tacgatcaga ttctcgacga cccttgtgtg gatgc cgtgt acatgccact 120 g 121 <210> 167 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 167 ctataaatcg aatcgattcc atctcatttc ctcaggcagc aatttccaaa gcgaaggcgt 60 wtttc a ggtga 211 <212> acca ggtga 211 <212> <212> DNA <213> CUCURBITA MAXIMA <400> 168 tctcgagttc ttcaccggat aacgccgcac aaatccagat ccaaaaccga aaacaaaaaa 60 waacctaata aatcgcaatg gagatgaaga agatcgcctg 120 cAt 211 121gctg MAXIMA <212 cgccgt> gcatgcatga agaagcagca aaaaggccac tgtttttcaa taaaataatt gccgaacgtg 60 yactaaagaa agagagcttt gcattttgaa tgagaaaacc tgctgaagaa gtgaactttg 120 c 121 <210> 170 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 170 aagatgagtc ctctgttgaa gtcgtcttgg gtcacttgtg aagccaaagc ttcggctgat 60 ygtaatgaac aagtacatta gtaacgatcg aaacccgaga cttaaacata gcaatggact 120 c 121 <210> 171 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 171 ctttgtttgt atattaggtt tgtattttct tgttggttta atatccgaaa agttgagtgg 60 ygtgacccct cttgtatcca tgttgggtt 121 ctggcct cttgtatcca tgtttagggtt 121 > DNA <213> CUCURBITA MAXIMA <400> 172 gccttttcat atctgcttga tgctctggca tacttaccag ctttgaacaa tacgtttccc 60 ycttccttct ttctccctgc tgcttctatt ttctcttccg ctgttttcag atcgaaagat 120 t 121 <210> 173 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 173 aattaagcag ctagaaggaa aaaagaaccc acagcaacat aagagaacaa aatgctctca 60 kattagatcc atcggcaaca atgaaataaa cattatatcg ccttgaacca atcaagatgg 120 g 121 <210> 174 <211> 121 <21 2> DNA <213> CUCURBITA MAXIMA <400> 174 ttgcggagga ggacgccatc ggtggtggag acagcaagct tcgactctcc acagcccatt 60 rttgaggcaa aattgatgga tgaagt 175 tgaagtggcg gaggaaggag aaattagaat <t C 121A 121 tat 175t DNA tgaagtggcg gaggaaggaga acccattttc gggccaccgt gaatgggtcg ggcaccgccg ctgttggtgg 60 mggaaagacg gtcgggcgtt ggctgaagga gcgcaaggag aggaggaaag aggaaaacag 120 a 121 <210> 176 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 176 aaatccgcgc agatctcctc tccggcgaag aatttcttgt cgccaaggca ttgttcttag 60 kaattcgatc gtctctgccc tagctttctc cctttcttca ctgctgcggg aaccgattaa 120 t 121 <210> 177 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 177 atctgttgca gcttataagc tttctcaatt gggagcgaac tggcaatggc gggaacttga 60 wtccacatca tcgtgcctgt tg 210 212 178 a tag 121 gg DNA <213> CUCURBITA MAXIMA <400> 178 gcatttggta ccaagtgagc agcttaaccc ctatatacac tgtatcctta cattcttgca 60 mtgcctttga gtacttataa gctaatttcc ggttatgtca gcaatattac acac ctacag 120 c 121 <210> 179 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 179 ttttcttcat cactttcccg cttaaacctt atcccaaaac cttcacttcc ttacgcgctt 60 matactttga aatcactag 211 ctact tc tca accagg 211 ctt 121 cc 212> DNA <213> CUCURBITA MAXIMA <400> 180 atgagggcat ttacaagaac gtctggttcg ggcatgccat gcagctccat ttaagctcta 60 yagttgagtc gagcacgaac tcaccatctg ttgcggattt atattgaatt tcaccatctg ttgcggattt atattgaatt t ttattgcaat caatcacaga agaactcaag gctgcaaaga aggatttggc 60 ygcaatacgg gatgaaggct ttcagttcat gacatcgatg gatgctgtac gaagggaact 120 g 121 <210> 182 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 182 cgtgtcaggg aagagaatgc taatttgaag aaacctgatg aaaaaactga tttagttgtt 60 maaaaactaa actcaaagct gcttagagca aaaaccaaat tggaggctgt atcttcagct 120 g 121 <210> 183 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 183 aaagagaaaa ctcacagcaa aagcaacgat tgctgaagat aaagcgagaa atccatactt 60 sgccatgcct tcagagag gc caacaaaggt gttcgcaaca ccgaacataa tcctccagag 120 c 121 <210> 184 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 184 tctctcatat gacattggaa tttgaaaacc accaaccttg tttatgtatt tgaaaagttt 60 yttttcttca gttgaaaact gccaagagtc tcgaatactg taatagtgac ttttgctgcc 120 g 121 <210> 185 < 211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 185 attaggattt ctgcgtgacg ttttgatatc tagtagctcg acggcttata attagtgttt 60 ytaggatcgc agctcaaggg gtcttctgtt UR 210Actt 212 gcttt t Aagt 212 213 gct <ta 400> 186 ttgccacatg ctagtgaatt ttttccagga cccttttgat ggatttgcag caatgaaaca 60 racagacgga taggttcagt agctcaggga atagaggcca aggagttccg ttcgtaaact 120 c 121 <210> 187 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 187 cgacttcacg aattgaggac ttcgattgca gcgacgattg acttcgccgc gagaatgagc 60 rgtgatccag ttcggtactc gcagagttct cattaggatt tggagtatcc atggcgaaga 120 a 121 <210> 188 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 188 taccagtttc agagacaagt acagcatggg ccacgctcgg ggat caggcg tggtggaaat 60 wcacaacctg tacatcagca gcaggtaaac tgaattacgt tgttaagatc atcaatctac 120 t 121 <210> 189 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 189 tggaagtcta gaatcacgac tgtcaattct atcttgttgc cgaatatttt cattcattcc 60 wgaaagatcc ctggatgaat gagctgtgcc atagcttccc ggccaagcag cagcatcaga 120 t 121 < 210> 190 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 190 agttgacacc cgccgtagat atcgatgcgg cggcaacggc ggacctggtg atgctggttc 60 sggtggtgga 213 agctgaaacc aagagattt 121a agtgtt agg > CUCURBITA MAXIMA <400> 191 attaggaggc tgcaaaatgg tggcagaaga agcagccatg gaacaagaga agctcgcatc 60 kcggaatcca aggacgattc cgatcacaga gactcagttc ctatcgtgga agcggcagaa 120 g 121 <210> 192 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 192 ggcggtggcc gggacgagga atcggattga tgatgcggct gagaagtgcg aggattgcgg 60 saagcggcgg cggaggaagg tcgcgatctt agggcttgaa atcggcgatc gagatcggaa 120 t 121 <210> 193 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 193 gtcattgc at gccaagtata gctgatcttg ttcttgtcta gcattttatt gcaaatatga 60 mgcctagtga taaactcctt tgcacttttg gaaagctagc acattgatat ctgccgtagt 120 t 121 <210> 194 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 194 gagtcagaag aagaagattc tgatgtaatg gtcattgaac cagaattgaa ttatatactt 60 ycttcagtgt taacaacctt gggaagggca ccagatattc agcgtggaat aacaagaatc 120 t 121 <210> 195 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 195 ggcagggaga ttagctgaag caagtaaact tgtagatgaa atgccaatgc cagcaggacc 60 ragcatttgg ggttcgttat tagctatgat>121 <400> > DNA <213> CUCURBITA MAXIMA <400> 196 ttcacaagga gaggttaaaa aactaattgg caggtttcct ggttccttca aagaaacctc 60 rgagtcgtcg atgctaccgg gccagaagtt tta t a tta t a gccaagtt ctcaaatcac agtatt 213 121 UCATABI 197 DNA t a t ag t a t a t ag c gaccataaaa gatggtgcag aggagcagca gaggggtgtg 60 yagcagaggg gtgtgtatgc tgagaaccaa gggttgttca ctgagcccat gaacgtagct 120 t 121 <210> 198 <211> 121 <212> DNA < 213> CUCURBITA MAXIMA <400> 198 ctgcccgcca aactgcccga caggtgtact cttctggata ttggtgaagg accaccatta 60 wattaacccc tttctcactc ttctctgccc gccaagctgc ccgacacacg tgacgactcg 120 a 121 <210> 199 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 199 ctgattccag agcttctcta ggacgagtcg aaacaagtgc catggggatc ttgtaatgca 60 wcaggacatt cacaaactcc tttgatccag ctcgtagtct gtaaattcca ccctgcaatg 120 c 121 <210> 200 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 200 ttacacagct ttgccaaggt cataaacaca agctcaggga gagatgaaac ttacttcttc 60 kttagaagac cgcgcttcga gaagattgat atgttcattc caagaaatcc agacttcttg 120 c 121 <210 > 201 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 201 gatctcaaag ctttaaaaag atttgtggtt cgtaagatat gcatgaaagc agcaaaaagt 60 sggagcgaac ctgacaaata tcatcaactt 211> 212> 121 c CUCURBITA MAXIMA <400> 202 tcccgatctg ttccttgatc ctaatcacca aatcggtaca ttctgttact cgcaatttct 60 kccgattgag agtttcgact ctgttctcgc tccgccaccg tcgccgccgc cgtctgaata 1 20 t 121 <210> 203 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 203 gcagcgaatc aagcagcctt ctctggtgaa ggagaagcaa atacggtaca gaaaattcgc 60 yaccgcaatc tcgaatatgg 212c <ggcg> > DNA <213> CUCURBITA MAXIMA <400> 204 ctgtctttgg tcgtaataat gttgcagctt tagtccaatt gcttactgca acctctctgc 60 stattcgaga gaagacaatc accataattt gcatga accataattt gcatg211 c caaaaaatgg agggtaatca agagtccatt gactggtctc 60 rtcgaagtac cattgagaaa gagggagaga gtgggtcaga gcgagccaat gacgatggac 120 c 121 <210> 206 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 206 ggttgtggat cactttcatc aggaattgag gtactcatac tagcaccttc atctgaccca 60 sttacaggca cgggcgcacg agaacgtaaa gaacgatttc ttgaaccaga tctcctacct 120 c 121 <210> 207 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 207 tatgatcagc tctctctttc gaccaatgtt gagtgtgcaa agaagccgtg gtcacaccca 60 ygctcgcttc attaccaaaa ttaag agcat tgtaactcat cagtttcgcg cctccgtcgc 120 t 121 <210> 208 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 208 tgatcgtgaa ggacgagatg tggaacggtt tcgccgccat tactatggca atggggagct 60 raagccagct gcaaaaggaa gcagaggggc agagttagta gaaggccttg aaattaaaga 120 a 121 <210> 209 <211 > 121 <212> DNA <213> CUCURBITA MAXIMA <400> 209 tatgtcttta agcttacaga cagactcatg tgtgatattg agctctgcca gtagttcgta 60 katgtaaggt ccaggcttag taaatcctaa ttcctt 210 IMgg 211 121 UR 212UCta MAXIMA > 210 gattggtggc caagatttgt gatttggggt tggccagaag aaccagagag acaaggcatg 60 rcaattgttt tggagggacg ctgtcgtaca tggcgccgga gacattgatt caccgcgtgc 120 a 121 <210> 211 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 211 ccgtcacgta gccgccgtac tgcgataacg aaaccctagg cggttgtcct ggcagccgtt 60 ygattttgtc ctgctgtttc cgtccggcct gatcgtcaat ctcgaccgtc ggcaaaacct 120 c 121 <210> 212 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 212 atattcgtga gaaagaagtg gaagatttat tttacaaggt attttttctt a attttgttt 60 rcgcatgacg ttttggcgcc tgtgtggagc gcttaaacca tgtaattgtc tgtcattttg 120 g 121 <210> 213 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 213 cacgatgatt agtttccggt ccaatgaaat cgcaattcta aacaagaagc atatggccta 60 yaatagctca acgtataatg gaaagctcag actaaatgac atgttcttgc agcaaaatgg 120 a 121 <210 > 214 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 214 ggaactcgga acctagaaat cgtttgtagt aaaatagatg ataatgagca attaggaatt 60 wcctgaaatg gaggacagac atcttggatt catgtggcag 212taatt 213 g 121 g CUCURBITA MAXIMA <400> 215 tctctttttc tttgccctgc tgcacgatct ttttctgatg ttcgaaacga cattttaaaa 60 racaaatggg agaaggaaaa acaggctagt ttatccatta cgagtgagct ttattctcat 120 a 121 <210> 216 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 216 cttgatccgg accaagtatg tcttttcaat aacttgcagc ttatgcattc tctaatgtgg 60 ytgattgctt gaaattcaga tccaacgacg gcgaccagat ctacgattga ttatctcttc 120 t 121 <210> 217 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 217 acagaactcg gaaga gacgg tggacgttag ccatgaaaga ctaaaaagat aaagccaaac 60 ygacaaaaca ccgctgtgcc gccgcgtcga cctccggcac agccgcgaat cagcagagag 120 a 121 <210> 218 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 218 caatccttcc attgttcttc atgtcctaac agcatgtaac ttggatccga aagaccttgc 60 raagttagag gcaagttctt acgttttcgc ttgttcgggt ttaccagcat tgttgtgact 120 a 121 <210> 219 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 219 tttctccttc tctttccttg atatttccgc ccaatccgtc gccgttcaga ctatggttca 60 mtttttttcgc ggc 211 ctg 212 c DNA <213> CUCURBITA MAXIMA <400> 220 agagagatgc aaaacatcaa tccagaaaca ggggaacgat gccaaaagga tgaaagcgag 60 raacgaatac aacacttcaa tcccctttcg gcaattggta ctattagact acagaagctt 120 g 121 <210> 221 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 221 gttcaccttc tctattatct tatgatgata aagacaactg aattcatctt gattcttaaa 60 ytgatatttt ggataattgt ctttccctcc ccaacagctt gaggatttag gtttcgacag 120 a 121 <210> 222 <211> 121 <212> DNA <213> CUC URBITA MAXIMA <400> 222 gctcaggtta gttttctggt ctctgaaaac caccttgaaa gttcatccga taattgttgg 60 rtcatggagt gctgcttatt tattattcaa ttgttataca ttgtaaagct atatatgaaa 120 a 121 <210> 223 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 223 cctgcatcca tggaagcggc aaccagacct tcaattcttc tcacactcgg ccgcatccat 60 mtatgagtgg cgtcttcatt aggtcaacca tcaaaaactt ccgtcgtttt gttcttcctt 120 t 121 <210> 224 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 224 gtttcatttc atgaatcttc caccgccaat ggctcttttg ccaactgccc aaccgttggg 60 ytccgggatc ggtggcagtg gcggcggtcc agccaccgac acccacttgg ggatgttagc 120 a 121 <210> 225 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 225 ttaagtgatt gcttatagca tgcataactg aagtatctct gggttattca caggctgcta 60 ktttgagaat cgctgagcaa tatatacaag ccttcttgca <213> 210 UCUR 212 Cactatatacaag ccttctgca <211> 121 UCURgtctctgca <tattgcca <211> <400> 226 gtaaaagaaa tttgatgaga actagagaag attatcatac ctggtgatgg caggggagaa 60 sctgatgctg caagatatga tcaagaccaa cgttaaaact cataaaatct aagaaattgt 120 t 121 <210> 227 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 227 gatgttgaat ccaaaagggg aagaaaaagg tgcgttttgg accaaaaatt gagctgtttt 60 rtcggtcaaaga aacaggaaca <210 act> 212 catta rtc tcggtcaaa aacaggaaca <gcagggtttg 211> 121 act 213> CUCURBITA MAXIMA <400> 228 accacatcgg tgtcttccaa tataacatca aggcattgaa tctacgcaat caacacaaag 60 raaaaaccct agttcatttc atatcccaaa aatcacaata aaaaatgcag tgatgaacaa 120 a 121 <210> 229 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 229 ggatgcgggc aataagaggt atagattgaa gggaggtttc gaagaaggaa gaggaggcag 60 rgatggggga agaaagaact cgcttgaaga gaagccatgc taattcgggg cttttggaat 120 t 121 <210> 230 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 230 tctaggcaca ttacccgatt tcccctgttt ccaggcagtt gaattgtcct caatagtcgc 60 ygagaccaac ttcgcgacct tctttgggaa tgacccatca aaatctgcag catcaacgac 120 c 121 <210 > 231 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 231 aaattcccga cacccaattc ttacttcttt caacaccaac cttccatctt ccctgtacga 60 yatgaagcaa accccaccct ttccactcct tc ccatgaaa gacaaaggcg gaggcaaagc 120 t 121 <210> 232 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 232 ttctcacctt tagaatagcc acctttcata caatatcaaatg cttcgctt tagtatg cttctattt ttg catagtat tactattt ttg catagtat tcatttt tg catagtat tactatttt tagact 121 <212> DNA <213> CUCURBITA MAXIMA <400> 233 gatttctatt ttcccaagtg gggaaagaac aagtaatacc cattgagaaa agacttgaaa 60 mcaagtctca gtttgctaca ttacgtatgc TAcgtatgc acaggcttct ca 234 ccaacccaga aaatccactg aagaacacaa gaaacaaaca aaaacccatt aattatttga 60 ygattacaga ggaaagaagg tttgaattaa tgaaattgat ggattacttg atcatcccag 120 g 121 <210> 235 <211> 121 <212> DNA <213> CUCURBITA <400> 235 tgattctggt caagatgagt tcgattacga ctcgactccg gtaccggagg atgtcaaaga 60 rggacatttt gcagttgtgg cagttgatgc tgaggagccg aaacggtttg ctgttccgtt 120 g 121 <210> 236 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 236 gtacgccatt gttaagattc attgtttcca tgtaccattc actgtctttg tcccaaagat 60 scttgtaatc gttgaggaaa tgagtagaga atttatcttt caaagggtca aagaaatctg 120 t 121 <210> 237 <211> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 237 tcgatggatg ggacattctg ctccaaagct cggatttcct tgtctttatg tactcacctt 60 ytaccataga agccgccatt gcggaattgt ggtctaccga agctgcctat tggagtctta 120 c 121 <210> 238 <211 > 121 <212> DNA <213> CUCURBITA MAXIMA <400> 238 gcgcttggct tggctacgga gggcggcagg accgactacg acgttgccgc agttgacggt 60 rgcggaggtg cggtcgagat agttggcgta 210 TAg 239 g MAXIMA <400> > 239 tgggtaagaa taggatatct gtacttatta tgtgaagata gctgctcttc tcactttcta 60 raatcacttt ctttactttc tcttacttag gctcggtttc ttatcgctct tcggcgcatc 120 c 121 <210> 240 <21 1> 121 <212> DNA <213> CUCURBITA MAXIMA <400> 240 aatttacggc aacaagaaaa gaaggatctc cgatacctgc gctgcagccg tcgacacctc 60 ygtcgattga cctcgtcgaa aacctccaac ggccgccgac gcct 120 t ccgccgac att gcct

Claims (6)

A composition for identifying trait differences between varieties of Western pumpkin, comprising an agent for detecting all single nucleotide polymorphism (SNP) located at the 61st nucleotide of each nucleotide sequence in the nucleotide sequence of SEQ ID NOs: 1 to 240.
The method of claim 1,
The formulation comprises a polynucleotide consisting of 5 to 121 consecutive bases comprising a single nucleotide polymorphism site located at the 61st base of each base sequence in the nucleotide sequence of SEQ ID NOs: 1 to 240, or a nucleotide sequence complementary thereto. A composition for identifying trait differences between varieties of pumpkin seeds.
The method of claim 1,
Wherein the agent is any one of a probe, a primer, and a combination thereof, a composition for identifying trait differences between varieties of Western pumpkin.
A kit for identifying trait differences between varieties of Western pumpkins, comprising the composition of claim 1 .
The kit of claim 4, wherein the kit is a PCR kit, microarray, or kit for Fluidime SNP analysis.
a) isolating a polynucleotide from a repeat parent western amber sample, and determining the genotype of all single nucleotide polymorphisms present at position 61 of each nucleotide sequence shown in SEQ ID NOs: 1 to 240;
b) isolating polynucleotides from backcross progeny Western pumpkin samples, and determining the genotype of the single nucleotide polymorphism in step a);
c) comparing the single nucleotide polymorphism genotype of the repeat parent determined in step a) with the single nucleotide long polymorphism genotype of the backcross progeny determined in step b); and
d) A method for selecting a backcross progeny of Western amber, comprising the step of selecting a backcross progeny having a high ratio of concordance between the repeat parent and the single nucleotide polymorphism genotype among the backcross progeny.

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