KR102461756B1 - Snp marker set for identifying cucurbita moschata cultivars and method for identifying cucurbita moschata cultivars using the same - Google Patents

Abstract

The present invention relates to a single nucleotide polymorphic marker set and its use for backcross generation-shortened breeding of oriental amber. It can reduce the required time and cost or increase the accuracy of judgment.

Description

SNP MARKER SET FOR IDENTIFYING CUCURBITA MOSCHATA CULTIVARS AND METHOD FOR IDENTIFYING CUCURBITA MOSCHATA CULTIVARS USING THE SAME

The present invention relates to a set of single-nucleotide polymorphic markers for backcross generation-shortened breeding of oriental amber and uses thereof.

The backcross method is a breeding method used to introduce useful traits of a particular variety into other varieties with excellent practicality. Finally, it consists of a process of selecting individuals that are similar to the recurrent parent but have useful traits of the introduced parent.

Conventionally, in order to select an individual having a desired trait, the phenotype of the backcrossed individual was confirmed. However, there was a problem in that it took a very long time because the individual was grown, the phenotype was visually confirmed and selected, and the generation was repeated until an individual with the desired trait was obtained.

Recently, in order to solve this problem, a breeding method using molecular markers that can select a desired individual at an early stage by identifying the gene at a stage before the growth of an individual is being developed. Marker assisted backcrossing (MABC) can be accomplished by selecting a backcross offspring that retains the target gene of the introduced parent, but whose parts other than the target gene have the genetic makeup of the repeat parent.

To this end, it is necessary to secure molecular markers that are evenly distributed throughout the genome, compare the molecular markers of the recurrent parent and the backcross progeny, and select backcross progeny with high similarity to the recurrent parent. It should be relevant. SNP is a molecular marker that can automate the entire process from detection to analysis, and has many advantages over other molecular markers in genotyping.

Although a set of SNPs for backcrossing using molecular markers for various crops have been developed, molecular markers that can actually help select backcross progeny for Oriental squash (Cucurbita moschata) are not known.

KR 10-2125528B1

An object of the present invention is to include an agent for detecting all single nucleotide polymorphism (SNP) located at the 61st base of each nucleotide sequence in the nucleotide sequences of SEQ ID NOs: 1 to 219, inter-cultivar traits of oriental pumpkin To provide a composition for discriminating differences.

Another object of the present invention is to provide a kit for identifying trait differences between varieties of oriental pumpkin, comprising the composition.

Another object of the present invention is to a) isolate a polynucleotide from a repeat parent oriental amber sample, and to determine the genotype of all single nucleotide polymorphisms present at the 61st position of each nucleotide sequence shown in SEQ ID NOs: 1 to 219 step; b) isolating a polynucleotide from a backcross progeny oriental amber sample, and determining the genotype of the single nucleotide polymorphism in step a); c) comparing the single nucleotide polymorphism genotype of the repeat parent determined in step a) with the single nucleotide long polymorphism genotype of the backcross progeny determined in step b); and d) selecting a backcross progeny having a high ratio of matching repeat parent and single nucleotide polymorphism genotype among the backcross progeny.

In one aspect of the present invention, in the nucleotide sequence of SEQ ID NOs: 1 to 219, it contains an agent for detecting all single nucleotide polymorphism (SNP) located at the 61st nucleotide of each nucleotide sequence, inter-cultivar traits of oriental pumpkin A composition for discriminating a difference is provided.

In the present invention, the identification of differences in characteristics between varieties of oriental pumpkin may be identification of not only varieties of oriental pumpkins, but also identification of lines of oriental pumpkins. The identification of the trait difference between the varieties of the oriental pumpkin may be identification of the trait difference of the oriental pumpkin. The lineage refers to individuals belonging to the same breed, but which can be classified by differences in detailed traits. The composition for identifying trait differences between varieties of oriental pumpkins may be used to identify or determine similarity to one or more of varieties, strains, genotypes, phenotypes, traits, and combinations thereof of Asian pumpkins. The composition for identifying trait differences between varieties of oriental pumpkin may be to identify the genotype of a selected single nucleotide polymorphism by detecting or amplifying a polynucleotide including a single nucleotide polymorphism site.

The nucleotide sequences of SEQ ID NOs: 1 to 219 are as follows:

SNP Marker Name Chromosome physical position Flanking sequence (downstream 60bp, upstream 60bp) SEQ ID NO: CmoMAB001 One 1090589 TGGGTCTTGGTTTGGTTGATAATGAGTTTATTTGATGAACAGGCAGCTGCACAGAGATGA[A/C]AATGTGTTGTTGCTGGATATAAGCTTCCTCACCCATTGAAGTATAAAATCATTGTCCGG One CmoMAB002 One 1152621 TCTGATGGCAACGAACATTCTTCAGCCGACAGTTTACAAGCCGTCGGTGAAGATCGCTGC[T/C]AAGGTAATCGGGCGTAAGTGGGCACCGTTGCCGGCGGTGATGGCGACGTTTTTAGTATCG 2 CmoMAB003 One 1495913 ACAAAGAAAGGGTATATTTTCTGTTGAATTCGTTTGAATAAGAAAAGCTATCTAGTTTTC[A/G]GATAACGTTTTTTATGAGATCAGAAGGATGAAAGTGTTTTTTTATTGTGAGATTGGCGCT 3 CmoMAB004 One 2586150 ACAAAGAGGAAGAACGCGTCGTGCTGCAGAGGAGAAGCAAATTACAGAAAGCCGATCCGA[A/G]GGGCCAAGGCTCTGTACCACGAAACCCATTGCTCGTTTTAGCAAAGCAAGCTTGGTACCA 4 CmoMAB005 One 2591211 CAAGGTTACATCTGCTGCTGGCGGAGGAAAATTTTGTGGAAAGTTCACACCAGCACGATT[A/G]TTCATGTCCATCAGCAAGTCTAGTGCAGACAAGGCCGTATTAGAAAAAGTAGGTAACTGT 5 CmoMAB006 One 2707460 GACTCACAGAAACTTCATAACATCTTCACTGATGATAACATTGGATCAGAAGACTAATGG[A/C]GACGAAAATGGCACATATCTGAACTTTTTGCCGATGTTTCATGTGTTTGGATTGGTTTGT 6 CmoMAB007 One 2888986 CCATGTTGAATCCCTTGGCCATGCTCTTCACGTTTTTATTAATAAAAAGCTTGCAGGTGA[T/C]TGGAATTAATTATTTGGTTCTGTGCATTTGAATGCCTTTAAAATTACTCCTGCGTTGTTG 7 CmoMAB008 One 3245142 ATCTCCTACCCCGATTAGGCAAAAAGGATCCGTTGCTTAGGAATGTCGATTTATTCATAT[A/T]ATTGCAACTTTTTCTTCAGGGGGCCTGTATTTTCAACCTCTTTCATGGAGCCTTCGCTTG 8 CmoMAB009 One 3928525 AGATCCATTGCTGCTGATATCCAGACCCTGTAATCGCCCAAGTGGATCACTCTGCATGGT[A/C]TCAGAGAATTGATGTGAATTCAGCTGATGATGCAGAGGATGTATTGACATGTTTGATGAG 9 CmoMAB010 One 10916851 TTGCTCGGACACTCTTTTCTTCCCCTCTTTTAGACCTTTATGATGAAGAGTGACAGAAG[A/G]TTAAAATGATGCAGGTGGAAATGTTAGAGTTCTCAGTTGGGAGGAAAGGATGCAAATAGC 10 CmoMAB011 One 11421464 GGAAGTCATTGAACCAACAACATTCATGACTCCCATTGCTACCATTTCCTTGTTCCCATC[A/G]ATTTGATAGTCTTTCATTGCAGCGAACGTTCTTCCGATCGCTACGGCTTCCTAAAAATCC 11 CmoMAB012 One 12189252 AAAGCTGGAGGCTCTGGAGTTCCCCCAGTGCTGCGTTGTAGTGTGTGTTATTATATGGGT[T/C]TCTTATAACCCTTTCTTTTTTGGATGAACTGAATTCCTCTCATGGAGGAGTTATGATGAT 12 CmoMAB013 One 12315694 CTCAGCATTGTCTTTCTCAGAAGTGTGGTTATTCGAATGAAGCAGCACAGAAGCATCACA[A/G]CCCTGTCTCATAAAAAACTCGATCAAAACTCGACCAAATCTCGAGAAAAGAACAATAAGA 13 CmoMAB014 One 12320956 TGGTGTGGCCTCTGAGACTTGATTTGTTCTGAATGACGTGGAAGTATAAATTCTGGGGGG[T/G]TTTTGTATTGGTACAGTACCGCAATTCAGCTTCAATTTGAGTGACTGGGGTTTGGAGCTG 14 CmoMAB015 One 13649233 GAATACCTTTTAAATATTTGATTTTTTTTGGGTGCAGATTGTGAGGGAGCAGCCAAACAA[A/G]AACTATTTCTCGGTGCTATGGGTGATACCCAAGTATTTTAGTGCATGGTGCGAGCTCATA 15 CmoMAB016 One 13973796 CAAATCTAGATCGTGAAAATTAAGCTAATGGACACAGCTTTTAAGCAAGATGCCAAAAGT[T/C]TACCTCTTCAATGGACTCGAGATAGTGAATAACTCCACCGTTGAGATCAGAATGATTCTG 16 CmoMAB017 One 14248204 AAGGAAAGAGAATACGAGTTGGGATCTGAAGCCTAAAGAATTGTTCGTTGAAGATATATA[T/C]TTCCCGTAGGCAACTTCTTAAATGCAGAAATAGAGAGAAGACGTGGGGGTTAGGTTTATGTA 17 CmoMAB018 One 14326418 AAAACCCTGAGAGAGAGAAAACAAAAAGAGAAGACAGTAATGGCGTGAGGGACTACAATC[A/G]GTTACTGAGACGCTGCATTTATAGACGGAAAAAAAAAAAAAAAAAAAAAAGTTAAAGCGGG 18 CmoMAB019 2 397288 AGAAGTTTCTTCCAGATTGATGTTTGGCGGAAAACTTGTAGGAGACTCCTGAGCTGGAAC[A/G]GGTGAAAGTGGTGCTGGTGGAGGTGGGGGTGGTTTGATACAAAAAATGGACTCTTTTTTT 19 CmoMAB020 2 485611 TCCCCTCGATGTTCCACCGCTGCCTTCCGTTCATCTCCATCCCTCTCTTATCTCTTCCTC[A/T]CTCTCAGAAGGAATCTATGCGAATTTATAGGAATGCAGGCGGAGACGACGACGGAACCGG 20 CmoMAB021 2 577489 CGGCAGGTAGACAGCAGCATTGCATACAAGCACTCAACTTCAAGAGGCATAACCGAGCGG[A/T]GGAAGCTATCCACAAATTGCCTCACACTGTCAAGCGGGCAAAGCCTAGATGCACAACAGT 21 CmoMAB022 2 581991 ATTAAGCCTTGCATTCTCTCCTCTCAATTTGAAAGCCTCACGATCGTAAGCCAAGGCAGC[A/G]TCTTCTGCAGTGTCGAACGTGCCAAGCCATAGTCGATTCCTGTTTCGAGGAAGACGAATC 22 CmoMAB023 2 706949 TGTCCACTCGTATGCTGCTGTGGCTTACTCAATAATTGGCTTGACCCACGCATGTCGGCA[G/C]AGCCGGTCTCCGGTAATTCTTCATCCTTTTTTGGTCCACTGCGGGTAATTAAGTCATTAT 23 CmoMAB024 2 3985334 GTGAGTTCTTCTGCAAGAGAGAAGCGAGTGCTGCCATTTTTCGAGGAGTATCGGAACCTG[T/G]GGAGCTTCTTGATAGAGGCAGGGAGTTGCAAGCTCTTGGCGATTTCAATCAAACTCTTCT 24 CmoMAB025 2 5576018 TCCTTATTCTAGGGTTTCCTGAACATGTATCCGAATTTGATGCAGTTGGTGGTGAAAATG[G/C]CGAGTCGAGGTTGGTAGCTGGATACCTCTCTCTAGCCATGAGCTCGGCTCTTCCTTTGCG 25 CmoMAB026 2 5576100 ATACCTCTCTCTAGCCATGAGCTCGGCTCTTCCTTTGCGTCTCTTATATCGCCGCCATGC[A/T]GCTTGAACAAAGCAGGCAGCCCATGTTCGCCATTGATGCGAGTAGAATCGAAACTTGTGC 26 CmoMAB027 2 8290850 CTATCCTAGATCCCCGCGCTCCACCAGCGGATCTGACGGAACCAAGCAGCAGAGCTACTG[T/G]AAAGAAGACGAACTCCAATTCAAAAATCGAGACAATACAACGGAGAAGCAATTCAAATAT 27 CmoMAB028 3 684318 TTGATGTCCCCTGGAGAGTGTGAGTATTTTCTTTACTCAATGTTTGTTTCATATCTAAGA[T/C]GTCATCTAGCATGGTGCTTTAGTAATTCTAAGACTATATTCTGACCTGCCTTCTTTTATT 28 CmoMAB029 3 3235775 TATCTTTGGGGAAGCCAAGATTGAAGATTTGAGCTCCCAACTACAAACCCAAGCTGCAGA[A/G]CAGTTCAAGGCACCTAATTTGAGTAACTTGACATCAAAGCCTGAGCCATCAACCGTGGTT 29 CmoMAB030 3 4494104 CACCTGTTTAGTTCATCACAATTTCAAATTTTCATTACTCTGAAATTCAATGAATTCAAA[A/T]TTTCTGTTCTATGAAAATAACCTACCTTGAAGTGAAGCAGGCAAGTGGGAGACAATATTGT 30 CmoMAB031 3 6078721 GGATGCTGCTTGCTTCTCTGGTTCATCTGTGGAGCCTAGAACTGCAGCAAGAATAGCCTC[T/C]TCAAAGTCATTGTCATCAGGCAATATTTCTGCCCATTTGTCTGTCTCCTCAGAGGTAGGA 31 CmoMAB032 3 6413365 GAAATCGTAGTACAGCTGAGGCTGCTCTGTGTCATTGAGGCTCCATGAGAGAAAGGCGAC[A/G]CCGGGATTCAGATCATTGGTAATTCCATGTGGGAATCTCGGCGTTTTCTGGCTGTGAGAA 32 CmoMAB033 3 7914935 CTCTGAAAATTCACCATCATCGGCTGCTTTGGATGTAACTGTGAAGTTGGTACTCACACC[A/G]GCTAAAACCTTGAGTAGACCTTGGAAGAGAGCAAAAAGATGCGACGAAACGCCTCCGATA 33 CmoMAB034 3 7915064 CTGCTCGTTCCTCCACCAGTCGTCGATCCCGACACCGCCCCACTGCATCTCGAGGATACC[G/C]GTCGCAGCAATTGAAATGAAGAGTGCCATGAAAATAAGACTAGCATAGTTGCTAATCTGC 34 CmoMAB035 3 8003317 ATGTCCATCCCTTTTACCTTATTGTACTCGAGTGACGAGAAAAAAACAGTAAAAAAAAGG[A/G]ACTTTTGCTTCATAGCTTTTCTCGTGGTGCCTTCGAGGAGTGAATTATGGGTACCCTTTA 35 CmoMAB036 3 9160666 CGTCGGCTCCGTCAGTACGGCGAAGGGAGGAAAGGAGTCATCGGCGTTTGATGTGGAGGG[A/G]AATGCAATTCGAAGGGAGGTGGAATCATTGCCGGAGATGGAACCGGCGGGGGTCGGGGAA 36 CmoMAB037 3 9790389 CAGCATTTGCCCTCCACCTGCTGCTTTTTTAGGCCCAAAATGTGCGCTTTGGGATTGCCC[A/G]CGACCTGCCCAAGGGATGGATTGGTGTGGGGACTACTGTAGCAGTTTTCATACTGCCTTA 37 CmoMAB038 3 10559460 ACCTCCACCAACCGGGCAGGTGATGAGGGGGACCGCCACCCCCACCCAGACCTGGAATGCC[A/T]GCGCCCCCGCCTCGACCGGGGATGCCTCCTCCACCCGGTGGGGCAGTTCCTATTTACGGA 38 CmoMAB039 4 1501965 TTTCATTTTTACTTCCAATCTTAATCTTCTTCCCTTCCGCATTATCATCATCAACTGAGC[A/C]ATTCCCAGTTTGTGAATCTCTTAGATTGCTCGTACTTAATGCCACTTGCAATTCTGAATC 39 CmoMAB040 4 2107990 AAGCCATTGATTACTTGAAGTCCTTAAAACATGAAGCAAGCGTTCTGGGTGTGAGCTGCT[A/T]TTAGATGACCTGCCCCTAATTTAGATGTTGGTGGTTAATTGGTTAAAATTACGGTGTCAT 40 CmoMAB041 4 2574022 GGAGATTTTGCGTATACTTTAGATGTTGAAGATATTGATGAGGACTCGGCTCTAAAGTTT[A/G]ACCTGACACCCGTTTATCGGGCATATCACATTCATGCAAGCCTTGGCACCCTAGAGCAGT 41 CmoMAB042 4 2590023 CGAGGCATTTGGTGGAGGCGAGCGAGGGGGCGGTGTTTGGAGCGATGGAAGGCGAGTTCC[T/C]GTAAACAGAGGACGCTGAGGCTGCGATGTAATGGACTCAATATGGCGCGCAGAGGCTTAC 42 CmoMAB043 4 3583900 GATCCCAAACAAGCGACAGCTAGGAGCCACCTCGGTTTTCCTCCCCTCTTCCCCAAACTC[A/G]TTTATTTGGTCACTCATTGGGTTCGATGTGCGTGGAGTTGGACATGTCGAGTGTGCTGTT 43 CmoMAB044 4 3702861 TTAGATTACCTTGGCAGAATCAAGCTGTGGTGTATCCGAACGAAAAGCCAAAACAAAACT[T/C]GATTGCTGTCTTTCACCACGATTTGGATTCCAAGATATACTTGCAGACAAGCACGCTGCT 44 CmoMAB045 4 4262843 TTATGGGCTTGGCTCTGTATCGTTGGTTGGGGCACCATAACAGATTGATATGAATGGTTA[T/G]TGGGAGCAGGATGCGGCTGCATAGACATGAAAACATTAGGATCTGAACCAATGGCCAACG 45 CmoMAB046 4 7287235 TCCAACAACATTCATTCCAACAACCACCCTTCCCTGCTGCTGTAAGCTCGATAAAGATAG[T/C]GGATCGTAGCCATTTCTTCTATGCATTTCGGAAAATAGATCGACTGGGAAAGGTCGCATG 46 CmoMAB047 4 7745958 CTATTATAGAAGGATTTTCCAGATTCTTTCAGATAAAGTGAATAGAAGAGAACGATAAAA[T/C]AAATATGCAATTGAGATGTGGAAGAAAGAACTTGATGATTACCGGAGCGAGTGTGGTTCT 47 CmoMAB048 4 7770793 CAAACCCGGTTCGCAGCTTGCAATCGTTTCGCTCGGAAGGAAGGAGGCAATCGCACAACT[T/C]CCTTTCATTACAATAAGTGGATGCATTCCTGGATTGATTAAATCTGGAGACTTGTTCGTT 48 CmoMAB049 4 7816936 CTGGCCGTTCGTCACGATCGCGCTCAAAATACTCTCAATACGCTCCACCAGTTCCTCATC[A/G]ACGCCCTCCGCTGCCTCCAATACGCCATCAATCGACTGGAGAAACTCCACATTCGAATCG 49 CmoMAB050 4 8667868 TAGGCTTGAAGGGCATTTCTCAATAAAAGGTGCCCAAAAGGCTGCACAATTAGCTGCTCA[A/G]TGCCTTAGCCGAGATCAAAAGATCAGACCTCTAATGAGTGAAGTTGTAGAAGCCTTGAAG 50 CmoMAB051 4 8716656 GGAATCATTTCCTCCCCTTTCTGCTACCCATTCCCAACGCCTACTTCAATAAACATATTG[G/C]CACCATCTTTGTGTCAGTGGATTTCAAATGCTGCTAGACGTGCCACTCTCTAACCAAGCT 51 CmoMAB052 4 9690156 TTAACCAAGGAGGCCTCAAGTTCAGAACTTGCTGCACAATAAACTACCAACTCAACACTT[A/T]CATTCCATAAACAATGTTTTAAGTACAGGAGGAGGCAACCCAAGAAGCTTGTCGTAATGA 52 CmoMAB053 4 10041831 CAGCGAGAAGGTGAGAGAGGGGAATGAGAGAGAGAGCTGTGAAGAGAAGTTGATAGGCCG[A/G]AAGTGGATTGGAGGTGTCGGAGACGGAGCCGGAGGAATCCATGAGAGGCGGAGGCCTGGA 53 CmoMAB054 4 10105971 GGCTTGGAGTGTTCGGTGGTGTTCATTGACTTGGATTGTCGTCTTGACATCTCAAGGCTT[T/G]CCCAAGTTTTGAAGCTTCGAATTTTGGAAGGAAATGGCAGCGGTGAGCTATTTGTGCCAT 54 CmoMAB055 4 12543688 ATGATATGGAAGATACAAGTTTGATGATGTAACATTAACGTTGTTTTGGAATCATGGCCT[A/G]AAGAAGGCCCATAAAGCAGCAATGTATGATAAGTAGAATATTGATGCTGCAATGTCTCTT 55 CmoMAB056 4 17956800 GCGAATGAGCTAGTGCAGAAGTTTCATATAGCAATGGAGACTGTTTCTGAAAACATTAAT[A/T]GCAGTGAAGCTGGAGATAAATTTACCCAGCAGCATAAAGAACTTCTGTCTGTTCTCGATC 56 CmoMAB057 4 18856327 CCTGACCTCGCCACTAGCACAAAGCATCTCTAGATATTTTGCTCTTAGTGGGTCCCGCAA[A/G]AGCAACGGCCAATTGGAGTGCAGCTCGAGATATGGACAGAATACGGATCTTTCGACTTCC 57 CmoMAB058 4 20510336 ATACAAATTATGCAATTATGTTACACATTCCAGTTGCTAATTTCCATTACCACAATTGGT[A/T]AAGTATCATTGTTCTTGTTTGTTATCTTGCTGCAGCTGCTCCTACATGCTATCGATACAC 58 CmoMAB059 5 272092 TTTAAGATGCTTTGTTGGGTCACTGAAGTCAAACAAAACGCAGCATAGATTTAGCTTCTT[A/G]ACAAACAAATTCTGTTTTTCAGAGCTTGGAACTTCCCTAAACCCAGGCAATGCCTCATAT 59 CmoMAB060 5 785878 GGTGGAAGATAATAAGGCAGCCACTCAAGTTAAGCCCAACATGGGAAGACACGTGGACGG[A/C]GGAGATTGGTCAAGAAAGCCAATAACATTATGGAGTCAAAATTCTGGCAAAGAAGAAAAG 60 CmoMAB061 5 910125 CAAATACAAGAACGGGTGGTCGGCAATGAAGTTGATTTGATCTAAAGCCAAGGAATGTCC[T/C]ATTACAATAGCTGTAGAAGCTGCAGCTTCTGTGCCTTCTTCATTAACCTCAATAAACGCC 61 CmoMAB062 5 1075438 CAAGAATCACTCCAGAAATCTCTCTTGCATTGTTGCTTCGATCTTTCTTTATTTTCTCAT[A/G]AAGGTTTCTTAGGCATGCTTCTCCAGCCAGTAACTCTGCCAGTGGATCATGGATCATGTG 62 CmoMAB063 5 1302409 AAGCGCCTTTAACAAGTTGCTTAACCGCCTCGCAGCTGAAGGCCACGATCTTTCAGCTCC[A/C]GTTGATCTTAAGAATAACTGGGCAAAGCATGGAACAAACCGTTACATCACCATAAAGTAG 63 CmoMAB064 5 4031250 CTAAGAAGAAAGTGAGCTACAAGGACGTTGCCAGTGAACTGCTTAAGGATTGGTAAAACG[T/C]GTGTTGGGTTTTACTTAATTTGTTTATATGTTTCTTATGTGATGGTATGAGAAAAATTCA 64 CmoMAB065 5 5280339 TTATGAGTCGGTATGATTCTGCTAGCCAGAGTCACTTTTCACTTCTCTTTAGATCAGTGC[A/T]GCAGACAATAATGTTCTAGTCATTAGTAGGGTAGAAACTGATGTCTCCAATTTTTTTTTTT 65 CmoMAB066 5 8279685 CTCCGTAGTTTTATAGCTCTAACTGATGAACACTAAGGAGGTGACTATTGGTGTATGCTA[A/C]TGTTAGACTACATAAGTAATATGGAACTTCATGACCGTGAAGCTGCTAGTATCTGGTAGT 66 CmoMAB067 5 9636227 TTCTCTTGGATGAAAACTGGGTTGCAAAGGTTTCCGATTTCGGGCTCTCGAAAACTGGTC[T/C]GGATATGGCTCATGGTCACGTTAGCACGGTAGTTAAGGGCAGCTTTGGCTACTTAGATCC 67 CmoMAB068 5 9788011 CGTGCAAGGAGATTTGGCCACTACGGAAGCAGCTGAAGCTGAAGCGAGCGAGGAAGCCAT[T/C]GGGTATTAGCTGAAAAGAAGCGAATCTGATTCGAGAGGGCAAGTGAAATTGATTCGCCGG 68 CmoMAB069 5 9872989 ATCAGAATGAAAATAGTAGTCTCCCCAAAGACGAGATGCAAACTTATCAGCATCAAAAGG[A/G]ATTCCATGAAGTTTGACATATAACTTTGCAAATGATTGCAAAGTAAATGACCATCCTGCA 69 CmoMAB070 5 9922107 GCTCCCGGCTGCCAACATCGACGCTACACTCTTCACCCATCTCTTCGCTGGCTACGCTCA[A/G]CTCAACTCCGGCACTTACCAAGTCACCGTTGATCCTTCACAGCTCATCCTCATCGATCAG 70 CmoMAB071 5 10080723 GGGTACAAGTTTGAAAAGAGAAATAATTGAAGTTTTCTGTGGTCATTGGCAGAGGCATTG[G/C]TACTGCGACAGATGAGACTGATATGATTTCTCACATTGTCTGCCATTTTTTCTATTTGAT 71 CmoMAB072 6 387469 TTTGAAGTTTGAATATTTCATGGATTGTAGGATGGTGCAGCAGGAAAGGAGTGGCGGCGG[A/G]TGTGGGGAAGATCGGGTGCTGGGAAAAAACAGAGGAGGTGATGGCGGCGGAGCAGTCGTC 72 CmoMAB073 6 759355 ATAGCAGTAGCCTAGTTAGTATTGTTGGACACCACAGGTGCCACTAGTCCCAAATGTTAT[T/C]ACGCCTACAGAGTACCCAAGGAAAAGTAAAACCTACCACAGTCCTCTTTGAATGACAATG 73 CmoMAB074 6 3554505 TTCAGGTCTCCAAAGATGTGGGAAGAGCTGCAGGCTACGTTGGACGAATTACCTGCGCCC[T/C]GACCTCAAGCATGGCCAATTTTCGGATATGGAAGAGCAAACTATTGTCAAGCTACATTCT 74 CmoMAB075 6 4370543 TTCATTCCTTTGATATGTCTAGAATCTGATGTCGGAGTGTACTTGCTTATCGCTAATACT[T/G]TGACTTGAAATTCAGCTGCTGTTAGTGAGAAGTCTTCGAATGGTTACTTGCTTATCGCCA 75 CmoMAB076 6 5423856 ATTAATTTTGGGCATTAAAGACCGTAAGCAAGAATTCAAGTTTTACCTCCAAATCTTATC[A/C]GATATCGAATTTCTTTGATTATGCACGTTCCTTAGGTCGTTTCTCTTCGCAACCTTCTTT 76 CmoMAB077 6 8873197 CAGAAGTGCTTCAGTAAATCTTGGCTAGCCAGGGTTGAAACGTGGTTCTTCACCCCGATC[A/G]TAATCGACCCCAAGAGAAACGTGCCTCATCATCTGCTCGCAGCTTACAGCCATCCTTCAA 77 CmoMAB078 6 9522921 TGTTTCTCTCTCAATCTTCTCATCTCCCAAGCTCTCATCTCGTTCTTTTGCCAATTCCTT[T/C]ATCTGTCGTTCTCTGAATCCCGCTAGCAACGAACGCGAGGAGGTTCGATGGATTCGTGAG 78 CmoMAB079 7 2129061 GTGATTCGTCGTGAGAACGATTGATCCTCTCATCGCCGCAGCTCGACCACAATCCATCCAT[A/G]AAATTAAGCATACCAGACAGCGTCAACTGCAATTCATCACACAAATTTCAATTTCGATTA 79 CmoMAB080 7 2866018 TTTGATGACTTTGCTGGCTTCTTGAGTTACTGGGTCGACAAGATAGTCAGGCACGTCACG[T/C]AGATGAGGAGCAGGGGGCTTCTTGCTGAGAATCTTATCATGTTTCAACAAATCTGCCCAA 80 CmoMAB081 7 3265582 GGAGATAAATTTCTGAATAGGAGTGTCAATCTTCTTAGATTTGGATAAAGATGTAAGCCA[T/C]CGGCAGCAGGGTGAACCAGTGGCAAGATGCTGGGTTGGAGGTAAACTTAAATTGAGCTAC 81 CmoMAB082 7 3409659 CAACAGTCTCCTCTTGAATTGTACTGAACGACCTATTCCATGCGGAATAATAGCCAATTT[G/C]GACCAGAATCAGCCGCAGAACTGCGGCTTTAATGGCAATAATTTGAGCTGCTTCACCGAG 82 CmoMAB083 7 4120945 CCCACCGAGTAAACAAAGAACAATGAGTGACAGACCAATCCACTGGTGGCCTTAGCAGCT[A/C]AGAAGCCCCCTCGGGCCCCAACGGAGGAAGTGGGTACAGAGAAGGAAAGGCCAACGCCAA 83 CmoMAB084 7 5685179 GCCTCATCGGTTGTGCAAACCTCTGAGCTGTGTTGCAATGTCTCTGGATGGCCGCTTTGT[T/G]GCTGCTGGAGAGGTGGTTTTTTTTTTTTTTTCCTTTTTGACCGTTTGTTTTTGTGGTTTT 84 CmoMAB085 7 5705313 ACTATGTCGTCACACTTTTCACAAAATGAAAGCTATCAAGGTGAAAGAGACTCAAAAAGA[T/G]TAAAGCTAACAAAAGAAGGAAATGATGACAGTTTTCCTGTTGCAAGTGAATCGCAGGAGC 85 CmoMAB086 7 7732120 ATCCCAGTTGGCTGCTGTTGACATATTTGTCAGTACTGTCGACCCTCTGAAAGAGCCTCC[G/C]CTTGTCACAGCTAATACGGTCCTATCAATTCTCGCTGTGGACTATCCAGTCGATAAAGTT 86 CmoMAB087 8 576267 AAAATTCATAGTGTTGGCTAGTTGAACCTAGTTTGATTTTTTAATTCTGGTGTTAGGTTC[A/G]GGCACATCATTCCTCTATCAGTGGCCTAATAACCTATATTAACTCTTCTGTTAGTTAGCTGA 87 CmoMAB088 8 721844 TTCTTATACTCATGTAGCTGCACTGAGGAAGGAGATCTCAGACGAGACTGATAGAGAGAC[A/C]GGGCGCAGTAAACAAATTTCAAGTGTTCCAATCCATCTGAGTATCTACTCTCCCAATGGT 88 CmoMAB089 8 1418400 GTGATTGCTTCGGTTAATCCTCCGTACACAATGCTCTTCACTATCTCGAAACAACGCCTA[G/C]GCTCGGGGCGATTTGATACAAAGGTCATATACACATTGCCTCTGCATTCAACGGAAATGC 89 CmoMAB090 8 3075350 TGCAGCTATAAATTGATCTGTTCTTTTCTGCCAGAAAGTGCTTTTATTTAACACTAGAGT[T/G]GTTGATTCTATTATAAAACTTCAGGTCTACATTAAAGCAACTGGAGAAAAAAGTGCTGAG 90 CmoMAB091 8 3112551 CTATTTCTTTGTTATTCTCCTTTGCAGCATCAGCCTTTGCAGCTAGTTATTATCCATATT[T/C]TCTTCATCTTCTTTTCTTTAAAAGTTCTACTTTGAGAGGTTCATAAACGATATAAGGCTT 91 CmoMAB092 8 6884211 ACACGAAATTGCGTGGCTGAAGTCGTCTTCAACCTGCAGCCAATATCCAGAGCATGATCA[A/G]AGCTGAAATGGCTATGGTTATCCAGCTTAGAAAGGCTAAAACAACGGAGATTTGAAACTT 92 CmoMAB093 8 6915297 GGGCATGGTGGACGACGTCACTGGCAATCCGCTGCTGTGATTCCACTTCATGGCCGGTTC[A/G]ATATGCTGTCTTTGACCGGTTCTTTTCTTCCCGGTCGAGTCCCTCCCGGTTCGAGCGGGC 93 CmoMAB094 9 461834 TTTCAAGGCCCTCCATTTCAAATTTGAGAAGACTGTTCTCTTAAAATGCGATTTTGTGCC[A/T]TACATTTTACAGGATCTCGGTCTCATACGTTTGTCAAGGATCGCATAGGCAGCAAGTTTA 94 CmoMAB095 9 773462 GGCGAAACTTAGAGAGATATCCCGAGGAGTTCGATTTCGTACTCTGAGTTCACTCGGATTTG[T/C]ATTGATGATTGTGGAAACAAGGATGATGGTGCTGAATTCGCCAAGTTGTTGGATAAATCT 95 CmoMAB096 9 910035 TGACTTTGATGAGTCTAGCTGTAGGCTTAATGGTCGTTGCACTTGTTACAAAGGATCTAA[T/C]GAGGAACAGAAATGCTGCAACTTGAGCCATCACAGAATTTCTTGTGCAGCTACGGTTAGG 96 CmoMAB097 9 1242227 CGATTGCCTGGCTGCAACTACCTGTGAAATTTGAAGAGCAGGCAACAAATGAACAATAAA[T/C]GTAAATTTGATAAAATTTCTCGTCCTAAACCCTAAACAAACAACAATAATAAAGGAAGAG 97 CmoMAB098 9 2056480 GATTGAATGAAGAATTCCCACTGGAGAGTGTTTCTTCTCTGCTTCCTTCAATCGTATCCA[A/G]TCACCAGCTGCTAACCCGAAACTCACCCGCTCAAGCGTAGAAGCACACACTCTTACAGGA 98 CmoMAB099 9 2074307 GTATCAGATCCCACCAGAGGTTTCGACCTGAACAGGGCTGCGTGTGATGGAAAAGATGTG[A/T]TTGGCTCAGATCCGAGGAGCTGTCTGTGTGCAGAAAGTACAAGCCATGGTAGACACAAGC 99 CmoMAB100 9 2176802 TATAAATCCCTTTACCCCTAAGTTTTGGGTACTGACCTCTTGCTGCATGATGATCATCCT[A/G]GTCGGTTGTTGCGAACAAAAGGCACGAGAATGGTGACGGTTGGACGGGGATGGAACGAAA 100 CmoMAB101 9 2196994 AGGGCTGCCGAGGCATCCCTCATTCCGCCCAGCTTCTTCCCAATCTGGGTCTCGCCCTGT[A/C]GAGCAAATGCGAGAGCTTGTGCAAAAATATGGGCAAAACCTATCTAACAGCCCCTCCACT 101 CmoMAB102 9 5244068 CGTATCTGAGAGTTATGTTTGCTTGCAGTTGCAGGGTCGAAAACCGGCTCAAAGGACAGA[A/G]TAAATGCTCAACTCCGATGCCCGTAAATAAGTTTCCATCAAGAAACAAGCTCTGCAAGTG 102 CmoMAB103 9 5263535 ACTTGGTTTTGTTGCTTGTTCTGTTCTTCTCATGGGTTAGCTTTAAGTTGAAGGCAGGCT[A/G]TGGGGGATGTCAAAGAGAGACGAGGTGTTTGTACTTTAAGGGAATGTTCATATGCTGCCT 103 CmoMAB104 9 5731264 ATATGAGAATTTTGAAAACAAGCGAAGTAAGAGAGGTAAAGATACATTTGGAAAGTCGGG[A/C]AAGAAACGGATCAATGGAGGTGATGAAGAATCGGATACAGGCAGGGGAGGAATGGATATG 104 CmoMAB105 9 6066333 GATGAACAGGGTTGGCTTTTCTTAACCACTTGAATTCTGTATCTAAGTAATCCTTACTGT[T/C]GTTTTGTTTCTTGTTTACATCTATGATTTGTTTCTTTGTTTCAATTGGTGGAATCTTCTG 105 CmoMAB106 9 6077762 AGATGCACTGCTACACGACTCGCCAAACGAATCCTCGTCGACAGATTCTAATTTCAGTAC[A/C]ACTGGCAGCAACAAGGATGCATCTCAGATGTCTTCACAGAATCCTAGAGAAATGCTTTTA 106 CmoMAB107 9 10677473 TTTTTTTTTTTTTTGCTGCAAGCAGTTGAGGATGACTGAACCCTCAAAGGTTATTCACGT[T/C]CGGAATGTTGGCCATGAAATATCGGAGGTTCATATCTCTCTCTCTCTCTATCCCTCTCAAGT 107 CmoMAB108 10 1050490 TTCAGGTCCAGAAACCTCCTGATTTCTGGGACTTGTTTTTTGAATTTGAATTGTTGCTCTG[T/C]TTTTTAATCCACTGATCAAATGAGATCTTTTATGCCCACCAAGTGCTTGACCTGATGCGA 108 CmoMAB109 10 1605505 GGGAAATGCTGCATCATCCATCAATTGGAAAGGGAATGGAACCAATGGAAACAGACAGTT[T/C]GTGGATCTGAGTACCTCCAAAGGAACTTCAAGTGTGGGTTGTAACACGCTCCTTGTTGAT 109 CmoMAB110 10 1936020 TTGTATAGGTGTGGGAGTATGTGCTGGTGGGGGAAAACAAACCATTTGCTACAGTCAATC[T/C]GTCCAGTGCCATCATCCAGTGCAAAAGTCACATCAGTAATTCTTCCTGCTCTATTGCGCA 110 CmoMAB111 10 2077280 TTTTTCAAGATTGAAATCAATCTCAATCTCATGGTTGTCTTCTATAGGAAGCACGGATGC[A/C]ACTCCTCCAGTTGATTTGTTTTTGTAGTCGGATCTTGCTTTAAGCGTGGCTGCTCCTTTC 111 CmoMAB112 10 2347744 CAGCTTTGTTTTTATGTTCATGGGCTTCCTTTTACTGAACCTTTTGTGGTATACTGAACC[A/C]TTTGGCTCTCTTAAAGGCAAGAGCAGGAATAACTGAGGTGCATTTCTTGCCGTTTAACCC 112 CmoMAB113 10 2740860 CTTCTGTTATTCAAACTGATGAGAATGACAACTTAGCAGCATCCAAGAAGATGTCGAGTTC[A/G]GGAAAATCTCTGTCGAAAACTCGAACTCTTAAGCCCCTTCTTGTCCAGCCAAATACTGTG 113 CmoMAB114 10 3048447 TCGGTCTCACTGCTCGTCCCGGCTTAGAATCTGGAATGGAGCTTCGTTGTTTATGAGTTC[T/G]GTTCTTCCTTGGATTAGTGGATGATACTTTTTCTTCATGCTGTGGTCTTGACACAACCTT 114 CmoMAB115 10 3177873 CATGTTAAATCTGATGCATCTCCACCAGATCTCCGGCAGTCTTAGTCGCCAAACGGCCAC[T/C]GACATAGCTCATTCCTTCGCTATTGCTCCACAGGATTACGAGTTTAGTTGCAGCAACAGC 115 CmoMAB116 10 3873000 CTGGAAAACTTTTCTTGAGAACTATGCTGAAGACTGGAATGATGTTGAAGTTATCGTTGC[T/C]TCACTCTACCTGAAGGATGCAGAAGAGGTAAAAACTTATATGATTGCATATGCCAAAAAA 116 CmoMAB117 10 4606788 TTTAGGAGAGAGGGCTGTTTTTGTGGATGAATGTTTATTTTGTAGCATCTCCCCCATTCT[T/C]GTTCAGGTTATTTGTTTTCCAATGAAGTAAATGTTCATTGACTGCTACTATAAATGTGGA 117 CmoMAB118 10 5520260 AACATAGGAGGAGTTCAGCCCCTGCAGCAACCCACCAGGTCGGACTGAAGATCTATCCAC[A/C]GACCAGTCTATGTCGGTGTAGTCTAAATTAGGCATCGAGCAACCCGTGTTCCAATCTACA 118 CmoMAB119 10 10779291 ATTGTTAACTGTATGTGAGATTCACTGTTCAGCACAGAATAGAATCTATGGAACGGAAAA[T/C]GATTGGTATGGGATTCTTCAAATTGATCAATCAGCAGACGAAACAATTATAAAAAAGCAA 119 CmoMAB120 11 1304212 ATTTAGTTCATTTCCCGATTCGATTTCGCAGCATCAGAACCGGTCAAGTATACATGATTG[T/C]ATATTCTTTTTCCGGGTTCGAAGGAAGGAAATTTCTCAACCAAGAAATGTTTCTTCTTCT 120 CmoMAB121 11 1307224 GCGCCCGCTTGTACAAGTTGGTGTTATATCACCAAGATAGTACTGAATACGTGTTATGAG[T/C]CAAGTAGCCTCAGGCAAAGGAGCTTTCTTCTGATTTTTTAGAGGCAGCACTCGCTGGTTT 121 CmoMAB122 11 3691960 CAACTTCTCCTCGCCGGCTGATGATTTCTCTTCCATTTCCGCAGCTCTTCCGATGGCTCG[T/C]AAAGCTTCGATTCAGCGTTTCTTGGAGAAGAGAAAAGACAGGTGGGTTTTAATTCAATTC 122 CmoMAB123 11 3959803 TCAATTACTAAGTATATATCTCTTCTCAACTTGAGCATGTAGGTGAAACTTATGTTTCAC[A/G]ACAAATAGGGCAATAAGAAATAGGAATCAGCACTTGAGTTGTGATATCTATAGCTGTTCC 123 CmoMAB124 11 4385220 CCAAGAAAACCAAAATGAAATAAACAAATTTGAAGTCGTAATTTAAAAAACGAAAAAAAG[A/C]AACTGGTGAGCAAACAAACACAAAGCTACCTATAATAACAAAAGAACAGCTGCATTGATT 124 CmoMAB125 11 4721538 GGAAAGCTCCGCCGTGATTAGGGCCTCTGGGACACTCCATATGAAAGGGAAAACGATGAA[T/G]CCAACGATGGCGAGGAGAGGGCCGGCAGCTTGGACAGCCGGCTCTTCGCCATAGGGGCCG 125 CmoMAB126 11 5144808 CCGTCACACATCCACTGCACACCCCATCGAACCCTCCGCCGTCAAACCCTGAGCAGTTCC[T/G]AACGGCCGCCTCGTACACCTTGCTCTGGAGGAGTGTTGGCAGGTAGAGGAGCGTGGAGAA 126 CmoMAB127 11 6145768 GAATGCCCATCGGGTCGGTTTATGGAGCGTTATCCCAGGAGGTACTGAAGACGTGATTAG[A/G]AGGAGGCCTTCGGATTTTGCTTCGAGTTTAGACATTTTGGGTACTAAACTCTATAGATTG 127 CmoMAB128 11 6194720 GAAACCTCAAAACAAAGAATCAAATCGCACAGCAGCAGAAACATTTTCCAGAGCCAGAAT[A/C]AAAAATACCTGACAGATATCGCAAAGAGGAGAATCGATTTTGATCGGCTGAAGTAGGGAG 128 CmoMAB129 11 9148097 CTCTACACTGTACCCGAATATCGCCGGCATTCTCCTGCATACCTTTGCTGCCTCCTCGTG[G/C]GAGAATCCCAACTCCTGCAAGAATCTGAATTTGCCTCTCAGCTTCTCCACCCGAGTATTC 129 CmoMAB130 11 10637252 CTGCGACTAGTGATCTGTGTCCCGACCCGGAAAGTTGGAAGTGAAATCTCCATTTCAGGA[T/C]GTTCTTCCCATTGTCTTGGAGGAATTTACAGGTAATTACTCTTCTTTCTGGTGCCTTCTA 130 CmoMAB131 11 11671486 CATAGACAGTGACAATGAGTTTAAGAAATCGGATTCTCATGGGCATATCGTCGACGCGTT[T/C]GGGGCGTCAATGGTAGAGCCTTGGCTTGCAGCCCATGGAGACCCAACAAGGTTTTCATTT 131 CmoMAB132 11 12677486 TTGGTTGTCGTAACTCGATGGCGGGGACGGAGGAGTTTCGAACTCGAACATACTTTCAGG[A/C]AATTCAGAAACATAGCCCTGGAAAACATAGTTCAATGGGAATTGTGTGGAGCAGCAGTAA 132 CmoMAB133 11 13231050 ACAAGTTCAAATGAAGAACACTCTACTCACACTATCTGCAACCGATTGAACGAGTTCATC[A/G]TAACTAGGATTGACAAGACCATCCCCAAACGGCACAAATTTGTAAGGAACTTCATATGGA 133 CmoMAB134 12 188855 GACTGGGCCTTCAACGGTATCGTTGCTACCACCGGGAGCCGCGGCCCCGTCGGTGGTGTT[T/C]CCAATCTTGCCATCGGCGCCGGGAGCCTCGGTTTTATTAGCACCGGCCTTGGGAGTTTCA 134 CmoMAB135 12 283076 CAAACTCCATGGATACAAAGATCCAGTGAAGTAAACTGATCTAAAACAGAAAATAAAAGA[A/G]AATAACCACCAGATCTGCAGCAAACACAAGCCGGAATCGAAAGCGGAAAAAAAAAAGAACA 135 CmoMAB136 12 2618023 GAATGTTTCGTTTCTTGGACATGCAGCGAGAGTAAGTTCTTGTGATTTTGTTTGTAGCGT[T/C]CTCGAGAACATACTCGAAGTTTTAGTTGTCTTTTGTTTTTAATCGATTCTAATAAAATCT 136 CmoMAB137 12 3068284 GATGCAAGGCTCTGCACTGCAGGGTGGAAGGATGGGAGTGGTTAGCTTAGCAGCTGGGGC[T/C]ATGCCTGTTGCAACAGGATCTCCAGCAAACCAGCTATCATCTGATGGGATTGGAAAGAGC 137 CmoMAB138 12 8941415 GAGGAAGCCTTTGATTGATGCTTACGACACGAAAGGAAAGACGTTAGACGACATTTCGGG[T/C]GATATAGAATTGAGAGATGTTCATTTTAGCTATCCTACGAGACCGAACGAGGCAATTTTC 138 CmoMAB139 12 9054111 AAGCACTTTTCCAACACTCCCAACGGTCATATTTCTCCACAGAGATTCACCAAAATCATC[A/G]ATGGCCGTTGTTTCCTGTTCTTTTAAACCCGAACCTCACTCGCATTTAATCGAAGCCCT 139 CmoMAB140 12 9389415 CTTCCCACACGGCGGAGCCATGGACTCAATGGATCATGGGGTTTACAGCGGGGGGTGGAAG[T/C]GGAGACGGTACGGCGGGAACTTCCTGATTTCGATGGCGGCCGGGGCGGAAGCCGGGAGC 140 CmoMAB141 12 11308110 TTGAGGATATCATCAAAGCAAACTAACGGTAACAGAAATGTTTAGAAAAGCTTACCGATT[A/T]GCAGCAAATGAAGTGGAGAAAGTTTCTACCTCATGTCCAACAGGCACGAAACTCCAACTG 141 CmoMAB142 13 5691576 CACCAAAAATAAGTATCCACATGAAGAAAACTAGATCAAATCAAAATGCTAAGCTACTTG[T/C]AAGTCGTAAGCCAAAAAGATTGTAATTGTGAAGAGTTAATTGCAGTTCAAATACTTGCAG 142 CmoMAB143 13 6150899 CACCAAAGGAGCAATTCTTCTCAAAATTTTAAAAGGTACTAAGTTGGAATCCCATTCTAA[G/C]TAAAGATTCTTGTGGTACAAGGACTACAAATTAATAGGAAGAAAAACAATGTTGACTACT 143 CmoMAB144 13 8072239 CTATGCTTCTCTTAGATTCTGCAATCATTCGAATCTGGGAATGAGAACTGCTAATAACAG[A/C]TGGAAATTGAGAAAGTACACACTACTACTTACATTGGAGAGTTCAATCAACAAATTAAGA 144 CmoMAB145 14 715343 TTTTGTTTTCTAATTCGATCTCCTCTGTCTCGTGATACAGAGCTTCGAAGACAACTCAAG[T/C]TACTCAAGTTAGAGACGGGAGCATTTGCTGAATCTGAAGAATCGCAGCACATCTCGAGTG 145 CmoMAB146 14 1078131 CTTCATTCGATTCCGCCTCCAAATTCATGAGAGCCACTTATCTCCTTTACGAGCCATCTC[T/G]CAAACTGTTCGAACAACTCACCCCACGTCCCTCCTGCATTATCTCCGACCTCTGCCTCCC 146 CmoMAB147 14 1094562 CTCCTTAAGAACGGACACAAGCTCAATATTTGATTCTTGACTTCTCTTTAGCTGCAGAGC[T/G]AAATCAGCATTGGATTCTTTTTGGAACTTCAGTTCATCTTTCAATTCATCTAAGATTTTT 147 CmoMAB148 14 2198689 TCATCATTCGCAGCAAGCTCGAGCAAGCTCGAAAAGGCATCTTCCGTTTCAACAGTCAAG[T/C]TATTCATGGTTGAGTCCAAGGGCTTAAGCAATCAAATGTACGGAAGAAATTACTCAAAGA 148 CmoMAB149 14 3014043 ATCGTATGGAGGACATTGCTAAGTGCATGCAGTGCTCGTGATGTTGATGGTGGGGCTCAG[A/G]TTGCAGAGGAGGCAAGAAAGAGACTCCTTGAGCTGGACGAAGAGGGGCGGAAATGTGG 149 CmoMAB150 14 3579878 CCATCCCAACTCACATACTGAGCTCTGTTTTCACAGGCATCTCCAAACACCAAGCTTCCA[T/C]TTATGGGTGCTTTTTGTCCCACACCACACATGGTCATACCTTACGTGGTATCCGCAGCACA 150 CmoMAB151 14 3839102 TGATAAACTTGGAAACAGATTTAGAGGTTATATGACAAAAAATGATTGAAGAAAAACAAA[T/C]TTTAGAGTCTAATTTTATGGGTTTGAAGTGGGATTTGTTGAGGTTTCAGGGTTCGTGATT 151 CmoMAB152 14 4165120 GAACGATAACACGTAGCTGCAAAATGGAGTTCAAATTTATCATCCAAACATGACAAAAGA[A/C]CCATACAAGCAAGCCATTTGACTAAGAGAATATGAACTGTCTTACAGAACAGTCCCAAGA 152 CmoMAB153 14 5219243 TGGTTTTTCTAGGTGGACTATGGCATTAGTAGCTATCTATTTTGCGGTATGTCTGTTGTG[A/T]ATTTGGTCTATATAGCTTACTTTCAATTTAGTGCCTAAAGTTTTGTGTAGAGTGCTTCCA 153 CmoMAB154 14 5494858 TAACTTTGAAAGGTCCGAAAAGTTGCAGCTCCGAAAGCAATTCGAATCAGATTTTTATTC[A/G]ACTTTTGGTTTTTCTTACGAAGTAGTCGGGTCGGGAGGTTTACAGTGTTTTTAACAACAG 154 CmoMAB155 14 10428586 AATTGTAGAAGTGAAGTAATTCATTGCTTGCTGTCAAGTTTGTACCATTATTGTTTTGGC[T/G]GCAGCGGCTGCTCTGGCTGTGGCTTGTTCTCCTGAATCATCACAGATATCATTAAGAAAA 155 CmoMAB156 14 10620077 TCCATTCATCCATTTATGTATACTCAATCTTCCTCCTCCAGACCTCCCTGCTCTGATTTC[T/C]CTTCAAATAATACAAACCCTCAACTCTTTTTGTACTCAGGGACTCATTCTCAAGCTGATA 156 CmoMAB157 14 12848662 TGGAACTGAGAACCAGCTAAAATTCCTTAAGCCTCTCTTCTTCTTCTTCTTCGTCTGCCC[A/G]GTTTGCGATTGAGAATGAGAGGCCTTCTACATCTACAAGAAAGAAATCAAGAAATTGGCG 157 CmoMAB158 14 12876076 ATGCAATCCGAGTCGAGGAAATTTGAAATCTCTATTTCCAGCAGGTCAGAACCTCAGTTA[T/C]ATTACATCAACTCAAACTGTAATTTTAACTTGATCACTTCTTGATATTGAAATGGCTGCT 158 CmoMAB159 14 13675030 ATTATCTTATCTAATGCTTATTATTTGCACAGCCTCAAAAACTTCATTTGATAATTTCTT[A/G]CCCAAAACTGTTACCCGCATAGTTTTGAAACTTCAGTTATCTCCATCTCTCCCTCCTGTT 159 CmoMAB160 14 13926713 ATATGTCGCATTAGCAAAAGTAGCAGCAATTACCATAATGGGTCCAGCAGCTACAAGCGA[G/C]CCAACAACGCTACCGCCGACAACCTGCCCTTGACCGCCGGACAAGTAAACGGTCAGGCCA 160 CmoMAB161 14 13945446 AGGACTTGCCGGAAATTTGCAGGTATATGTGCTGATTTTGAACCAAAACAAAATACTCTG[T/G]TATTAACCTCTGTTTTGTGCAGAGATTTCTGGTGGATTACTCAAAACGGGTGATGGAGA 161 CmoMAB162 14 13993588 GATGGTACAATTCACTATCCTTCAATTGATTCAAGTCCGATTGACTTGTCATCTCCAACT[A/G]CCCAGACAGTTCCCTTACAAACAGGTGAAGGAAAAGAGGAAACGAGTTCTTCTTCATGTG 162 CmoMAB163 14 15538056 CACAAACTGAATGATTGCAGCTGAACCCAGAAGTTGGAGAACAGTTTCCATCTGTTGAAG[A/G]AGATAATCTAAGACTTATTATTATTGGTTCTCTTGATATAGTTTCATAGGCAAAGGATAT 163 CmoMAB164 14 15614225 TCTTTTGGCATCTTCTTGGACAAAGCAACGATTAACAGTCCTCTGAAAACCAGCCCTTGC[T/C]TCTGCTGCTGCCATCAAACTCCTATCTTTTGAACATTCAAGCAGAACTTGTTAAATTTCT 164 CmoMAB165 15 727661 TAACCGAAGGATGATTGCCGAAGCAGGAGCAATACCGTTTCTGGTGACGCTGTTGAAATC[T/C]GGTGATCCAAGGATCGAAGAAAATGCAGTGACTGCATTGTTCAACCTTGCAATCTTCAAC 165 CmoMAB166 15 1039250 AATCATAAGCGTAAACGCCTTTTGTTAATGAAGGACAAGCTGCAGGTTCAACTGCTCGAA[T/C]AACGGGGCTTATTTTTCCAGCAAGTTTCTCACGGAGGAACGGGAAGCTAAGACCTGCAAA 166 CmoMAB167 15 1928169 TTCTTCCTTTGCTTTCATTCGGAAGTCATCGGATTGACTGTGTAGTCTACTGAAAGTCTG[T/G]GAATGGGAACGTTCACTTTTGGGATCAGATGTCAAGAGAGGCGAAATAGGTGACTCAGGG 167 CmoMAB168 15 2654492 AAATGGCTGCTCAAGAGGACTGAAACCTACCACCAGTCTTGGAGGTTCGAAATTCTTCCC[T/C]AAGCCTTCCCTTAAAAGCATCAGTAAAACTTTCAGGACCGTAAGTAGACGGAAGCTAAAA 168 CmoMAB169 15 2663910 AAGGAAGAAGATGTCTCGGGCTCAAGATTGCATCCTGAAATATATGTTGAAAATGATGGA[A/G]GTCTGTAAGGCTCAAGGATTTGTGTATGGGATCATTCCTGAGAAAGGAAAACCAGTAAGC 169 CmoMAB170 15 3377715 ACCGGCGACACTCGGCTTCAAGCTTCGCCATTTTCTTTATGCTCTCAAGATGTTGCTTAC[A/T]GGCTGTTTCAGCTGTTTGTGTACTCAAATCCCTCTCAATAGTCCTGGTTTCTAACTCCCT 170 CmoMAB171 15 3627784 ACATTTCTTACCCGAAATTGCAGCATTTAAGGCCGGTCTTCTTCTGCTCCCACAACAGTT[T/G]ACACCCGCCGTAGATATCGATGCGGCGGCAACGGTAGACCTGGTGATGCTGGTTCCGGTG 171 CmoMAB172 15 8317535 TCAAATGACAACATTCTCTGTCTCCCAAGCCACTACTATGAACCGACACATTGGAAAATC[A/C]TTTCAAATTCCGGCAGCCTCCCTCACTGCCTTCTTCGTTGCTAGCATCCTCGTAACCGTC 172 CmoMAB173 15 9545919 GCTTATTTATTTGCGACAAAGGGTCTAATTGGCAAACACTGCAACCACTGCAAGCACCTT[T/C]GGCGTGTCAATGGCGAAGCTGTATGCGTCGTGTGCAGCAATGGTGGCCGTGCAAATTGGG 173 CmoMAB174 16 218672 GGTGTAAGATAGATTGTGGTTCTGTAAGACCATGCCTCCTGTATCAAGCTTCCTTTGAAC[A/T]GTTCTGTGATGCTGCCATTTCTTTCATCCTCCATGCTCTCTCCATGTCCTTTGTTTAATGGC 174 CmoMAB175 16 320626 TCGAGCTGCTTGTTCTCGCAAGCGATGGACTTTGGGACGTGGTACGCAACGAGGTATGCT[G/C]ACTACATCATTACCCCATGTATCTAAGATGCTCTAAATTGTGAACTCCACATACGAACTT 175 CmoMAB176 16 985688 GGATTAACAAAGGAGGCAGTTTGATGAATAATGTGACATCCTAAGAGCAGCGAAACGCAA[G/C]TTCCTTACCTAGTGAGCATTTTCAGTACACAGCCATTGGTCATATCCATTTTGACATTGT 176 CmoMAB177 16 2163635 TGATCATTGTAAATGGAAAATTACAGAGAAATTTCAAAAACGGAATTATAGCAACGACTT[A/G]TACCAGATACTGGAGCATTAGCTTTCCCAGCATAAAAGGTCTTGGCCCTGACGACATCAA 177 CmoMAB178 16 2194949 TCCACCAGCATCCGACGACTTGGCGTACAGGTGCTCATTGAGGCATAAAAACAAACACCT[A/T]ATGGGCACAAGCTCTGAACTAATTTCATGGTCCCCAAAGCACGATGCTGCAGTTGTATCT 178 CmoMAB179 16 9148953 ATCATCTTCGGCCGAAAACTCCTCTGTTTCGTCGCAGACGATATCGGTGTCGTCAAATTC[A/C]CCGCTGCCAAGCTGACAATGGGGGAGTCATTAGTAATGTCGATAAGCGCCGATCGATCTT 179 CmoMAB180 17 805942 TATGATATTCATGAGTGTTTGAATCAACGGGTTTGATATTGAGTTACCATTTTGATGAAT[T/G]CCCATAAGGTATTTGATGAAATTCCCAGCTGCCATTTTTAGTTTTTTGTGCTTGAAAAAT 180 CmoMAB181 17 1000285 ACTATGGTCGTGACAAGTCCCAATTCAGTATACTAATGAATGATTTCTTACTGGTGAGTT[A/G]AGCAAATTTGTTTGCACTAAGCCAATTCATTCGTGTTCTTGCAAACGTCATATATTTCGT 181 CmoMAB182 17 2303559 AATTATTCAGCGTTGACTAATTGTATTGTCCCCAAGTAATGATAAATGCATGCGAGCAAA[A/G]ATATGAAAGAGTATTTGTGTATCACAGAATCTGGACTCGATATAAGGGATGAGAGCAAAT 182 CmoMAB183 17 2436117 TGACGTTTTCACTGCAGATTTCAATTCCAGTAAAAACCAGTGCCAATTTTCCTCCGTCTC[G/C]GCATCTACAACTGCAAAAGCTGCTGGAAATATGCCATCTTCCCCATCCACAGCTGTGGCA 183 CmoMAB184 17 7746738 TGACTGTATCTCTATAGTCAATAATTCTTCAAGGATCTTGATCAAAGACATTGCCTGTGG[A/G]CCAGGCCATGGAATAAGGTAACATAAATCTTCACTAGTTAGATCACTCCGATTGGATCAA 184 CmoMAB185 17 7774365 GGTTTAGTTTAGCTTTTTTGATTAAGATCCTGTTAGTTTTGTTTGTGTTAATTGAAGGAT[T/G]CTTGAGAACTTCTCCTCCAGGCTGATTTCCTGTAGCTGCCATGTTCTTCGTTGTTCAATT 185 CmoMAB186 17 7814226 CAATTAAGACCTCGAAACTCCAAACTCCAGATCGGTTCCAGAGATGAAAACTCTATACAA[A/G]ACGAACTGGTAAGAGGAAGAAGCTCGGAGCTTCTTCAATGGCGGTTTCTAGGGCTTTAGG 186 CmoMAB187 17 7898700 ATTAAATTCACATTCGAGAGGAGTGGAAGCAGCCGTAGGATTGAAGCAAGGGTAGGATAG[T/G]AAATTAAGACAAAAGTGAAGGGCAGGGGTCAGTCGCACGTGTGAAAAAGATTGAAGTCAC 187 CmoMAB188 17 8488983 AATATAATTTGCTTTAGAACATAAAGCAGCAGAAGAAAGAAAACACGAAAATCAATCAAC[T/C]AACAACTCCCAGATATTTCTAGAATTGTAAGGAATTAGACCAACCGGATGGGATGATCTC 188 CmoMAB189 17 8804499 ATACAGGTTGATCACTCTACAAAAGGTGAATTACCAGCCAAACGTTGTCAGTGCAAGAATG[A/G]AAGAAAATGGTGAACTTGGTTTCTTGGTTGGTGCATTGCATGTTCTTCCTCTCTATGCCA 189 CmoMAB190 17 10271888 ATATTTGGCATAGACGAAGGTCTTTAGTTGGATGGGCAGTTGTCTCCAACTCACAGGCTT[T/C]CATCGAGTGCCCATCAGTCCTCTGATTTGAGGTGAATTCTGATGCAGTTGTGATCATTGA 190 CmoMAB191 18 856797 AGATTCAGATTTACAGGTGATGAAGGAGAAATTAAACCCTGCAATCTTCCAGGGAAACGG[A/G]GAGGTTGAAGGTAAGAAAATCAGTGGGAGAAGTAACGGGGAAAGTTTCTCGGTGTCGAAG 191 CmoMAB192 18 1238500 GTTCGAAATCGTAATCGCCGTCGACCCATGAATCGCATCGATCAACCCATCATTACAGTT[G/C]GACAACGAGCAGTGATCCACCCAGACATGGCTGCCGCCGAAGATTGAAACGCCGTCGCCG 192 CmoMAB193 18 1280175 CATTAGCAATGTCTATAGCCCCCGTCGTCGACAGAGACGATATCGGCTGCGTGAGGAGCT[T/C]AAACAACCAAACCTACTATCACAATCTTGCTCTCAGGGCCTCATTGCAATCTTTAAGGAG 193 CmoMAB194 18 1593939 TTGCGAGGGCATCTGGACCAGCAGCAGCTGCTTCCTCGGCCTTCTGTTTCTCTTCCTCGA[G/C]TTTGAACTTCGGCTTCCATTTGGTGTCGCGGTATGAACTAGCGAGTTTCTCGTCTTTGGC 194 CmoMAB195 18 5506608 ACAGATGCCCTTCTTCATTGACATTTGTCAGTCTGTAGAAAGATAGCCAAGCATGACAGC[T/C]TTGGATAAATTTGGTGACTAAACTGTATAAAATCTTTAAAAAGTCCTTTAGTCTTACATT 195 CmoMAB196 18 9378712 CATCTCAGAATACACCGTTGCTCAGTATAATTCGTCACGTCACCTGTCGGGTCGTTTGAA[T/C]GAAGTCTGGATCGACGTTTCAGCTGTTACATCAGAGGGCTGTCAACGCCGGTAACGAGTT 196 CmoMAB197 18 12459404 CTCTGACATTTTTCTTCTCAGCTGCGGCTCATCCTCCAGTCCCACCTTCTTTAACCGCAC[T/C]TTCGTCGGCGATTCAGTCAAACCCGCCTCCGACTTTCTCACTGCCGGCCGATCGGTGGCG 197 CmoMAB198 18 12731613 GTGGTGGGTTTTGCAAAACTCCTCGAGCTGCTGGACCTGGGAGAAGTTCTGAAAGTGAGG[A/G]AAGAAAACCCCAATATCCTCGAACATTTTGAGTGCCCAATGAAATCGAAATCTCTTAAAA 198 CmoMAB199 18 12860094 TCGGTACTTACTGCTGCAGACAATTGATTGCATGATTCCGGAAGGCTCCCAGATGGATTA[A/C]AAAATTGACCTGCCAAGAACAATTAGCAAGATTATTTTAACAAGATATGTCATGGGCGCG 199 CmoMAB200 19 139944 TCGTAGAGCTACAAGAACGAAGATGATGATTCAATGTTTTTTGACATTTGGCTTCTGAAC[T/G]TTTAAATGCTTGCTCTGTCTTTGGTTTTTGTAGTTACAGATCTTTATATTGTTCATCGAA 200 CmoMAB201 19 324575 ATCAAACCGGATCTCCAAGTGATAGCTAGAGAGAGAAAAGATTGATATCTAATACTCATTGG[A/T]ATCGTGGCACGAAGAACTTGGATCGGACAAGGCAAGATCATCGTTGGTAACCTAGAATCT 201 CmoMAB202 19 751302 AAAAACACTAGCTGGTTTGTTGCCATCGAGAATCGGTTTCCCGAAACGATCGGTAACATC[G/C]GCGGTTGTCATGATGGCTGATTTGATAGCTGCCGGTGTCCATTTTGGATGAGCTGAATGG 202 CmoMAB203 19 2203566 GGTCGACTCTGTCTCGACTCTCGGCTTCAATCGATTGGTCCGATCCTACTACGAGGACTG[T/C]GGCATCTGCTGATGCTGCTATCTTCTTTGCTTCATCTAGCTGGGCAGAGCTGCAAGCCAC 203 CmoMAB204 19 5662972 TTGATTTGATGTTTGTGTCTAAAAATGCAGCTTTCCCTCGAGGGAGCAATGGTCCATCTA[A/G]CCTCTCGTTACAAGCTAGTCAAAACGAAAAGACTGAATCTCATGCACAACAAGATCAAAA 204 CmoMAB205 19 6395915 TCAAAGACTGCACCAAATGAGTCTGAATTCTCCATTGAAGATTGATGGTCTTTTGCACAT[A/T]GTGAGTTGCAGCACAATTTTCTTCGTGTACTTTATTGACCTTTGTGCTTGGCTTGGCTAG 205 CmoMAB206 19 6728098 CACCCAAGTCGGCCCCGTCTGCCGGAGCTTGATCGTAAAAAAGATTCTCGACGTTTCGGG[T/G]AACTGCATTCTCGGGCTGCCGGACCAGAAATCTAAGGAGCAATGTACCCATTTCTTCAGC 206 CmoMAB207 19 7621315 GGATTGGGAGTAGGTTTGAAGATGATAAAAGAAACAACCCAAAGAGATTCCCCATTCAAC[A/G]AAAGGCAGCTGTGTTCTTGTGTGGATACCCACCACAAGCTCCTTACTTGTAAATACCAAA 207 CmoMAB208 19 7737787 CTATGTTGCAAATCTAGGTGATTCACGTGCTGTTTTGGGTAGAGTAGTGAAGGCGACTGG[G/C]GAGGTTCTCGCCGTTCAGTTATCGACAGAGCACAATGTTTCTAATGAGTCGGTGAGGCAA 208 CmoMAB209 19 8033113 GCACTCTCGACCGAGCCCTAAATACCGTGAACGAAAGCAACAAAGCTTCGCCGATTCGGT[T/G]CTGCAGCGATTGGAGGATAAGTGATGTCAGCCAGTACCTAGTCTTGAAACTGCTTTTACC 209 CmoMAB210 19 8523415 TATATACACTCATCCATCATATACACTCATCCATCGCCCTCTCGCTCTCTCTCGTGCGTT[T/G]TGTATGTACAGGAAGATTGTAACAGTGGAAGAGATCTTAACAATTGACATAAAACCAGGT 210 CmoMAB211 20 124230 GTTGGAGCAGCTCTTTGACAGCTCCCTCGTGGGTTGAGTATTACTCATCTATTTGTAGCT[T/C]TCCACTTATCAATGGATATTTTCTCGTTTCTTATCTAAAATATTTGCCGTCAACTTATCC 211 CmoMAB212 20 2042637 AGTTTTAGCCCTAAAGAAAGTTGTAATTTCAAAGAAATACCCTCAACGAACGGCTGGGAC[G/C]CTCTCCAGGGAAGTGAGCTACCTGACCTGAACCATTTTTGGGCGTGCAGCGTCTGGGTGTG 212 CmoMAB213 20 2079385 GTTTCATTCAATGATGCTTCGTACAACATGATCGGAAGAACAATCTAAAAATCGGCAGCA[A/G]CAACAACAACAACAACAACAACAACATCATCATAATGCCGCCTAGCGTTACGAAGAATCA 213 CmoMAB214 20 2167111 ACAGAATCAAACAGTCTCTATGAGACTTATAATCACCACTCATATGGTTCTTCACCGGCC[G/C]ATTCGTTCTTCGATGGAGTTTCATCGCCAGATTTCTCCACTCCAAACATGGCTGATTCAA 214 CmoMAB215 20 2830849 CCAAGTTCTTGACGATTCAACCGACCCTGTCATTAAGGTACTCAAAAACTCTTCTAATCT[T/C]CCGACTCTCATCGTTTTCAATCCCCAAATCACAAAAACCTGATTTCCCAACACGAAACCC 215 CmoMAB216 20 2834224 AAACATCCTCTTGAACAGAACTTAATTATGTTTGGCAATTGTGAAAACTGAATGTTGTGT[T/G]GTTATTTTGTTTGAAATGAGGACAGAATCAACACATTGGAGCTGGGATTTGCAGCGTTCC 216 CmoMAB217 20 3384658 TTCATAATAATAAAGAAAAACTCAGTCTATTTGATTCCTAAAGACCAAGACTGCAAAAAT[A/C]GCAAGCCATTCAAGTTTCACTCACCTTTAAGCAGGCCAAGGACAACAGCTGCTGTATAAC 217 CmoMAB218 20 3901630 TTATTTATAATGCGGGACATTATCTCCTGCAGCCGGAAGCTTGGACATGGATCGATCAAT[T/C]CATTGATAAGAGTCTAAAGATCTTTGGCAATGGTGTGAATACCCGATTTCGGGAGGATCG 218 CmoMAB219 20 4092624 TCATTTACTGGTGATCGATCATCATTGATATTTGAACAGCACGTACCTGGAGAGAGTGTC[A/G]ATGAGCTGAAAAAGAAAATTCAGTCACAGGTGATTTTAACATGCTTCAATTTGTGTTATC 219

The single nucleotide polymorphism (SNP) refers to insertion, deletion, or substitution of one nucleotide in DNA. The term 'polymorphism' refers to the placement in the sequence of genes that vary within a population. Polymorphisms consist of different “alleles”. "Allele" means a gene corresponding to an allele located at a region corresponding to each other in a pair of homologous chromosomes, i.e., at a homologous locus, and refers to two or more of a gene occupying the same chromosomallocus. It means one of the above alternative forms. The placement of such polymorphisms can be identified by their position in the gene and the different amino acids or bases found therein. These amino acid variations are the result of two different alleles, two possible variant bases, C and T. Because the genotype consists of two different distinct alleles, any of several possible variants can be observed in any one individual (e.g., CC (denoted A), CT (denoted H) or TT (denoted by B)). Individual polymorphisms are also known to those skilled in the art.

In the present invention, the single nucleotide polymorphism may be located at the 61st nucleotide of each nucleotide sequence in the nucleotide sequences of SEQ ID NOs: 1 to 219.

The 61st base is the 61st base in the nucleotide sequence of any one of SEQ ID NOs: 1 to 219. The nucleotide sequences of SEQ ID NOs: 1 to 219 include a single nucleotide polymorphism and a flanking sequence present on the chromosome of an oriental amber, respectively. In SEQ ID NOs: 1 to 219, each of the 61st bases is a single nucleotide polymorphism present on the chromosome of an oriental amber, and those associated with traits useful for breeding are selected. Analysis of the allelic type of the single nucleotide polymorphism may be useful for selecting oriental amber having a desired trait by comparing the base difference between the parents and the progeny. When used for backcrossing, the generation of backcross by comparing the genotyping results of single nucleotide polymorphisms of the repeat parent and the offspring of the backcross for all or part of the 219 single nucleotide polymorphisms, and selecting the offspring with high similarity to the repeating parent It can reduce the number of tremors.

The preparation may include a polynucleotide consisting of a nucleotide sequence identical to or complementary to a continuous nucleotide including a nucleotide corresponding to the selected single nucleotide polymorphism. The continuous base may consist of 5 to 121 bases. For example, the agent is a polynucleotide consisting of all or a part of the base of any one of the alleles located at the 61st position in any one of the nucleotide sequences of SEQ ID NOs: 1 to 219, and a polynucleotide consisting of a sequence complementary thereto, or a polynucleotide consisting of a sequence complementary thereto may be nucleotides. In the nucleotide sequence of each SEQ ID NO: A single nucleotide polymorphism is located at the 61st base, and a flanking sequence of 60 bp each in the upstream and downstream directions from the 61st base is linked to the SNP. One single nucleotide polymorphism is located in the nucleotide sequence of each SEQ ID NO. The length of the continuous base may vary depending on the SNP genotype determination method.

According to one embodiment, the agent may be for identifying a single nucleotide polymorphism located at the 61st base of SEQ ID NO: corresponding to the polynucleotide to be detected or amplified. The agent may be a primer comprising all or a part of a base of any one of the single nucleotide polymorphic alleles corresponding to the 61st base of any one of SEQ ID NOs and a side sequence continuous thereto.

According to one embodiment, the agent may be any one of a probe, a primer, and a combination thereof.

The term “probe” refers to a polynucleotide that specifically binds to a particular target sequence. The probe may be complementary to or capable of specific binding to a polynucleotide including a single nucleotide polymorphism site. The probe may have a difference in binding strength depending on the difference in bases of single nucleotide polymorphisms. The probe may be labeled to confirm the presence or absence of a specific nucleic acid.

The term "primer" refers to a single-stranded polynucleotide capable of serving as an initiation point in the polymerization of nucleotides by a polymerase. The primer may be prepared using a method known to those skilled in the art according to the selected SNP analysis method. The primer sequence may be identical to or complementary to some continuous bases of the base sequences of SEQ ID NOs: 1 to 219. The length of the primer may vary depending on various factors such as temperature and the use of the primer. For example, in the case of a primer for HRM (High Resolution Melting) analysis, it may be a primer pair capable of generating an amplification product of a size (eg, 50 to 500 bp size) that is easy for HRM analysis, including the base of the polymorphic position. .

The primer may be a primer for Fluidigm SNP analysis. Using the Fluidigm system, two alleles, SNPs, can be detected rapidly in large quantities. Fluidime system uses LSP (Locus Specific Primer), ASP (Allele Specific Primer) 1, and ASP 2 as primers and optionally uses STA (Specific Target Amplification Primer). The ASP1 and ASP2 may be prepared corresponding to the allele of the single nucleotide polymorphism. The 5' end or 3' end of ASP1 and ASP2 may have the same or complementary base to the allele of the single nucleotide polymorphism to be analyzed, respectively. Fluidime SNP analysis can use FAM and HEX as probes. FAM and HEM may be connected to any one of ASP1 and ASP2, respectively. For example, ASP1 and ASP2 are specifically hybridized to two alleles of the SNP, and the result is amplified, and the genotype of the SNP can be confirmed by concurrent amplification of the FAM and HEX sequences linked to ASP1 and ASP2. . A primer for the Fluidime system can be ordered and manufactured by Fluidime.

Another aspect of the present invention provides a kit for identifying trait differences between varieties of oriental pumpkin, comprising the composition.

In one embodiment of the present invention, the kit may be a PCR kit, a microarray, or a kit for Fluidime SNP analysis.

The PCR kit may include a primer set capable of detecting the single nucleotide polymorphism. The PCR kit may further include a DNA polymerase, a dNTP mixture, and a PCR buffer solution. The DNA polymerase may be, for example, a klenow fragment of E. coli DNA polymerase I, a thermostable DNA polymerase, or a bacteriophage T7 polymerase. The PCR buffer may contain KCl, Tris-HCl and MgCl2. In addition, the PCR kit may include components necessary for the identification of the amplification product. The PCR kit may include a guide. The guide may be a printout explaining how to use the kit, for example, how to prepare a PCR buffer solution, suggested reaction conditions, and the like.

The microarray may be a polynucleotide capable of detecting the single nucleotide polymorphism, for example, a probe immobilized at a high density in a divided region on the surface of a substrate.

The Fluidime SNP analysis kit may include primers for the Fluidime system, and specifically may include STA, LSP, and ASP 1&2 primers. The kit for Fluidime SNP analysis may further include a DNA polymerase, a dNTP mixture, and a PCR buffer solution. DNA polymerase and PCR buffer solution may be understood with reference to the description of the PCR kit.

Another aspect of the present invention is a) isolating a polynucleotide from a repeating oriental amber sample, and determining the genotype of all single nucleotide polymorphisms present at the 61st position of each nucleotide sequence shown in SEQ ID NOs: 1 to 219 step; b) isolating a polynucleotide from a backcross progeny oriental amber sample, and determining the genotype of the single nucleotide polymorphism in step a); c) comparing the single nucleotide polymorphism genotype of the repeat parent determined in step a) with the single nucleotide long polymorphism genotype of the backcross progeny determined in step b); and d) selecting a backcross progeny having a high ratio of matching repeat parent and single nucleotide polymorphism genotype among the backcross progeny.

In the method for selecting backcross progeny of oriental pumpkin, polynucleotides are isolated from the introduced oriental pumpkin samples, and single nucleotide polymorphisms present at position 61 of any one of the nucleotide sequences shown in SEQ ID NOs: 1 to 219 are selected from the group consisting of determining the genotype of one or more selected single nucleotide polymorphisms; The method may further include comparing the single nucleotide polymorphic genotype of the introduced parent with the single nucleotide polymorphic genotype of the backcross progeny.

The composition for identifying trait differences between varieties of oriental pumpkin according to one embodiment can determine traits or trait-related genotype differences by discriminating single nucleotide polymorphic genotype differences, using molecular markers to backcross (MAB) or breed judgment, etc. can be used

When the composition for identifying trait differences between varieties of oriental pumpkin according to an embodiment is used for oriental pumpkin backcross, individuals having a similar SNP genotype to the recurrent parent can be effectively selected.

When determining the similarity of SNP genotypes of oriental pumpkins using the composition for identifying trait differences between varieties of oriental pumpkins according to one embodiment, it may be associated with the similarity of oriental pumpkin traits, so the time required to determine the similarity of oriental pumpkin traits It can reduce the cost and increase the accuracy of judgment.

1 is a genotype analysis result (group A and group B, some) of 38 core Asian amber lineages.
2 is a genotype analysis result (C group, part) for Asian pumpkin F ₁ varieties (3 pieces) and parental lines.
3 is a genotype analysis result (group A, group B, and group D, some) of 41 strains of various breeding materials used in oriental pumpkin breeding.
4 is a genotype analysis result using an oriental amber SNP molecular marker (CmoMAB011).
5 is a physical map showing the location of 219 oriental amber SNP marker sets whose efficacy has been tested.
6 is a genetic phylogenetic tree analysis and classification results for Asian pumpkin core lines and breeding materials (total 79).
7 is a genetic phylogenetic tree analysis and classification results for the oriental pumpkin F ₁ and parental lines (total 9).

Hereinafter, one or more specific examples will be described in more detail through examples. However, these examples are for illustrative purposes of one or more embodiments, and the scope of the present invention is not limited to these examples.

Example 1: Materials and Methods

1-1. test material

In order to select SNPs that can be used for various breeding combinations of oriental amber, 226 amber resources were collected and 38 core oriental amber lines with no overlapping characteristics in various traits were selected.

As materials for the test of the marker set to be developed, 4 varieties of Zucchini, Great Moss (oriental) F1, 12 their parents, and 41 remaining breeding lines were used (group A, group B, group C, and group D).

1-2. GBS analysis of oriental amber core strains, NP search and selection between strains

High-purity gDNA was isolated by the CTAB method, and it was confirmed that the A260/280 value was 1.8 or more and the concentration satisfies the criteria of 100ng/μL or more using Nanodrop.

A GBS library was constructed using the restriction enzyme Ape KI, and the library was subjected to nucleotide sequence analysis for each core line of Oriental amber using Illumina Nextseq 500 equipment.

SNP calling of the core line was performed using the SAM Tools program, and SNPs satisfying the selection conditions were selected through SNP filtering.

1-3. Developed in the form of a Fluidigm marker set, the strain and F _One applied to the breed

The selected SNP was developed as a custom primer (or probe) set for the Fluidigm ^® EP1 ^TM Genotyping System, and using this, it is possible to assay multiple molecular markers on multiple samples within a short time.

The designed SNP marker set was applied to 38 core lines out of 226 collected amber resources, and genotype analysis was performed on 4 F ₁ varieties, 8 lines that were their parents, and 41 lines of various breeding materials used in oriental pumpkin breeding. was carried out.

Through the genotyping results of high-throughput marker sets, SNPs showing polymorphism between strains used in breeding oriental amber were additionally selected, and through this, marker-assisted backcross (MAB) breeding This is possible.

Genetic phylogenetic analysis was performed to analyze the genetic diversity of each lineage.

Example 2: Study Results

2-1. Selection and collection of oriental pumpkin core strains

Among the 226 resources including various characteristics such as fruit size, fruit type, skin color, leaf shape, leaf pattern presence and thinness, etc., which are the characteristics of amber, 38 were selected as the oriental core line and young leaves (new shoots) were collected (Group A). and group B).

2-2. GBS Analysis and SNP Search of Oriental Amber Core Lines

To construct a GBS library by extracting high-purity gDNA, it was fragmented by read using Ape KI restriction enzyme. Different adapters were attached to both ends of the fragment and ligated with T4 DNA ligase.

The nucleotide sequence of the constructed GBS library was sequenced for each amber resource using Illumina Nextseq 500 equipment, and then the adapter sequence was removed through the trimmomatic program and trimming was performed. As a result, the average number of reads for each of the collected 192 resources (group A) and 34 resources (group B) was 1,765,076 and 2,523,739.

The read mapping was performed on the standard genome using the Bowtie2 program, and the average overall read mapping rate was 84.0% for group A and 81.8% for group B.

Using the VCFtools program, SNP calling was performed to search for genomic variation information between the standard genome and Asian resources (167) among the collected amber resources, and as a result, 10,549 final SNPs were searched.

The number of SNPs for each chromosome was calculated using an in-house script , and the ratio of home/hetero genotype for each sample was also calculated.

2-3. High-throughput custom SNP selection and marker set development

A total of 10,549 SNPs searched in groups A and B were selected for genotype results with a depth of 5x or higher, and those with different genotypes between lines were classified as SNPs to select a total of 2,693 SNPs. SNPs with homozygosity of 0.96 or higher were selected. 2,214 were selected.

Among the selected SNPs, 396 SNPs with PIC (Polymorphism Information Content) values of about 0.35 to 0.5 were finally obtained in order to select SNPs suitable for tasks such as backcrossing group or purity test as much as possible.

A Fluidigm ^® SNP Type ^TM assay primer (or probe) set that performs PCR amplification based on flanking or amplicon sequence information, which is a 60 bp base sequence adjacent in both upstream and downstream directions from the SNP position, was prepared. Through the D3 assay design site, 380 were synthesized except for the 16 that were impossible to design.

2-4. Efficacy test by applying SNP marker set to oriental pumpkin lines and varieties

The 380 designed Fluidigm ^® SNP Type ^TM assay was applied to various breeding lines and F ₁ varieties used in the development of Asian pumpkin varieties to test their effectiveness.

Genotype analysis was analyzed for 38 core lines and 4 varieties of F ₁ , 8 lines that were their parents, and 41 lines of various breeding materials used in oriental pumpkin breeding ( FIGS. 1 to 3 ).

As a result of the analysis, it was confirmed that 219 of the developed 380 Fluidigm marker sets, except for 161, which showed a heterozygote genotype in most strains or were ambiguous to be detected with a fluorescent probe, functioned as molecular markers (FIG. 4). A physical map was prepared for 219 sets of SNP markers whose efficacy was tested (FIG. 5).

Information on the final 219 SNP marker sets tested for efficacy by genotyping is shown (Table 1).

It was confirmed that SNPs showing polymorphism between lines were selected using the developed 219 Oriental amber SNP marker sets, and individuals of the backcross generation similar to the genotype of the recurrent parent were selected early and used for generation-shortened breeding (Figs. 1 to 3). ).

In order to analyze the genetic diversity of each line used in the analysis, a phylogenetic tree (Dendrogram) analysis was performed. Phylogenetic analysis was performed in Darwin6 software (http://darwin.cirad.fr/) using the neighbor-joining method. The structure of the phylogenetic tree was based on an unweighted neighbor-joining method, and bootstraps were determined to be 1,000 replicates.

As a result of analyzing the genetic diversity of the oriental pumpkin core lineage and breeding material of group A, group B and group D, they were largely classified into two groups. All of the nurturing materials were included in the first group, and the core lines were all classified in the subgroup (FIG. 6).

Genetic diversity was analyzed using a total of 219 marker sets for a total of 9 parental lines and oriental pumpkin F ₁ in group C. Both F ₁ and parental lines were genetically similar but classified ( FIG. 7 ).

The above results can be summarized as follows:

Thirty-eight oriental amber core lines with various traits (e.g., fruit morphology, fruit color, leaf plaque and ultrafine) were selected, and DNA was extracted from young leaves to construct a GBS library using ApeK I restriction enzyme. The nucleotide sequence was analyzed using Illumina Nextseq 500.

380 ^SNPs were selected based on criteria such as sequence depth, polymorphism information content (PIC), ^homozygosity , separation ratio, and SNP proximity, and 220 SNP primers (or probe) set was developed.

The developed marker set was applied to 91 of 95 resources. As a result of testing a total of 41 breeding strains such as zucchini and rootstock (oriental), and 12 oriental F ₁ and parent strains, which were applied to 38 core strains and used for breeding by seedling companies, 219 primers out of a total of 380 It was confirmed that (or probe) functions as a molecular marker.

Through these results, the 219 marker sets described above show polymorphism for each breeding line, so they can be utilized as Marker-Assisted Backcross (MAB).

So far, with respect to the present invention, the preferred embodiments have been looked at. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

<110> Republic of Korea (Management: Rural Development Administration) <120> SNP MARKER SET FOR IDENTIFYING CUCURBITA MOSCHATA CULTIVARS AND METHOD FOR IDENTIFYING CUCURBITA MOSCHATA CULTIVARS USING THE SAME <130> RDA-P200046 <160> 219 <170> PatentIn version 3.2 <210> 1 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 1 tgggtcttgg tttggttgat aatgagttta tttgatgaac aggcagctgc acagagatga 60 maatgtgttg a tttgctggat ataagctt 121g <400> 213> CUCURBITA MOSCHATA <400> 2 tctgatggca acgaacattc ttcagccgac agtttacaag ccgtcggtga agatcgctgc 60 yaaggtaatc gggcgtaagt gggcaccgtt gccggcggtg atggcgacgt ttttagtatc 120 g 121 <210> 3 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 3 acaaagaaag ggtatatttt ctgttgaatt cgtttgaata agaaaagcta tctagtttttc 60 rgataacgtt ttttatgaga tcagaaggat gaaagtgttt ttttattgtg agattggcgc 120 t 121 <210> 4 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 4 acaaggagagca g agacgca atccga 60 rgggccaagg ctctgtacca cgaaacccat tgctcgtttt agcaaagcaa gcttggtacc 120 a 121 <210> 5 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 5 caaggttaca tctgctgctg gcggaggaaa attttgtgga aagttcacac cagcacgatt 60 rttcatgtcc atcagcaagt ctagtgcaga caaggccgta ttagaaaaag taggtaactg 120 t 121 <210 > 6 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 6 gactcacaga aacttcataa catcttcact gatgataaca ttggatcaga agactaatgg 60 mgacgaaaat ggcacatatc tgaactttttt 210 gccgatgttt 210 gccgatgttt ggt 212 <gtttg < 120 gatt gg CUCURBITA MOSCHATA <400> 7 ccatgttgaa tcccttggcc atgctcttca cgtttttatt aataaaaagc ttgcaggtga 60 ytggaattaa ttatttggtt ctgtgcattt gaatgccttt aaaattactc ctgcgttgtt 120 g 121 <210> 8 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 8 atctcctacc ccgattaggc aaaaaggatc cgttgcttag gaatgtcgat ttattcatat 60 wattgcaact ttttcttcag ggggcctgta ttttcaacct ctttcatgga gccttcgctt 120 g 121 <210> 9 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 9 agatccattg ctgctgatat ccagacc ctg taatcgccca agtggatcac tctgcatggt 60 mtcagagaat tgatgtgaat tcagctgatg atgcagagga tgtattgaca tgtttgatga 120 g 121 <210> 10 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 10 ttgctcggac actcttttct tcccctcttt ttagaccttt atgatgaaga gtgacagaag 60 rttaaaatga tgcaggtgga aatgttagag ttctcagttg ggaggaaagg atgcaaatag 120 c 121 <210> 11 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 11 ggaagtcatt gaaccaacaa cattcatgac tcccattgct accatttcct tgttcccatc 60 ratttgatag tctttcattg cagcgaacgt tctt 12ggctt 121 ccta aa atctt 120 c 121 cc gct <213> CUCURBITA MOSCHATA <400> 12 aaagctggag gctctggagt tcccccagtg ctgcgttgta gtgtgtgtta ttatatgggt 60 ytcttataac cctttctttt ttggatgaac tgaattcctc tcatggagga gttatgatga 120 t 121 <210> 13 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 13 ctcagcattg tctttctcag aagtgtggtt attcgaatga agcagcacag aagcatcaca 60 rccctgtctc ataaaaaact cgatcaaaac tcgaccaaat ctcgagaaaa gaacaataag 120 a 121 <210> 14 <211> 121 <212> DNA <213> CUCURBITA MOSCHAT A <400> 14 tggtgtggcc tctgagactt gatttgttct gaatgacgtg gaagtataaa ttctgggggg 60 kttttgtatt ggtacagtac cgcaattcag cttcaatttg agtgactggg gtttggagct 120 g 121 <210> 15 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 15 gaataccttt taaatatttg attttttttg ggtgcagatt gtgagggagc agccaaacaa 60 raactatttc tcggtgctat gggtgatacc caagtatttt agtgcatggt gcgagctcat 120 a 121 <210> 16 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 16 caaatctaga tcgtgaaaat taagctaatg gacacagctt ttaagcaaga tgccaaaagt 60 ytacctcttc aatggactcg agatagtgaa taactccacc gttgagatca gaatgattct 120 g 121 <210> 17 < 211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 17 aaggaaagag aatacgagtt gggatctgaa gcctaaagaa ttgttcgttg aagatatata 60 yttcccgtag gcaacttctt aaatgcagaa atagaga <212> DNA SCHATA <400> 17 aaggaaagag aatacgagtt a 400> 18 aaaaccctga gagagagaaa acaaaaagag aagacagtaa tggcgtgagg gactacaatc 60 rgttactgag acgctgcatt tatagacgga aaaaaaaaaa aaaaaaaaaaa gttaaagcgg 120 g 121 <210> 19 <21 1> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 19 agaagtttct tccagattga tgtttggcgg aaaacttgta ggagactcct gagctggaac 60 rggtgaaagt ggtgctggtg gaggtgctggtt BIa 210a 213 SCHata <400> CATA 121 MOSCHATA <400> t t TA 212 <212 SCHagg gg tggttttgggcgg aaaacttgta 121 400> 20 tcccctcgat gttccaccgc tgccttccgt tcatctccat ccctctctta tctcttcctc 60 wctctcagaa ggaatctatg cgaatttata ggaatgcagg cggagacgac gacggaaccg 120 g 121 <210> 21 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 21 cggcaggtag acagcagcat tgcatacaag cactcaactt caagaggcat aaccgagcgg 60 wggaagctat ccacaaattg cctcacactg tcaagcgggc aaagcctaga tgcacaacag 120 t 121 <210> 22 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 22 attaagcctt gcattctctc ctctcaattt gaaagcctca cgatcgtaag ccaagt 211 agat gta c 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 23 tgtccactcg tatgctgctg tggcttactc aataattggc ttgacccacg catgtcggca 60 sagccggtct ccggtaattc ttcatccttt tttggtccac tgcg ggtaat taagtcatta 120 t 121 <210> 24 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 24 gtgagttctt ctgcaagaga gaagcgagtg ctgccatttt tcgaggagta tcggaacctt 121 <212> cagattggttct ggtagat tc aggaacctt 121 caggat c <212> DNA <213> CUCURBITA MOSCHATA <400> 25 tccttattct agggtttcct gaacatgtat ccgaatttga tgcagttggt ggtgaaaatg 60 scgagtcgag gttggtagct ggatacctct 210 ctctagc 400 gagctc atacctctct ctagccatga gctcggctct tcctttgcgt ctcttatatc gccgccatgc 60 wgcttgaaca aagcaggcag cccatgttcg ccattgatgc gagtagaatc gaaacttgtg 120 c 121 <210> 27 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 27 ctatcctaga tccccgcgct ccaccagcgg atctgacgga accaagcagc agagctactg 60 kaaagaagac gaactccaat tcaaaaatcg agacaataca acggagaagc aattcaaata 120 t 121 <210> 28 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 28 ttgatgtccc ctggagagtg tgagtatttt ctttactcaa tgtttgtttc atatctaaga 60 ygtcatct ag catggtgctt tagtaattct aagactatat tctgacctgc cttcttttat 120 t 121 <210> 29 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 29 tatctttggg gaagccaaga ttgaagattt gagctcccaa ctacaaaccc aagctgcaga 60 rcagttcaag gcacctaatt tgagtaactt gacatcaaag cctgagccat caaccgtggt 120 t 121 <210> 30 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 30 cacctgttta gttcatcaca atttcaaatt ttcattactc tgaaattcaa tgaattcaaa 60 wtttctgttc tatgaaaata cctaccttga agtgaagcag <212> DNASCHATA <400> 30 cacctgttta gttgg ga <400> 31 ggatgctgct tgcttctctg gttcatctgt ggagcctaga actgcagcaa gaatagcctc 60 ytcaaagtca ttgtcatcag gcaatatttc tgcccatttg tctgtctcct cagaggtagg 120 a 121 <210> 32 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 32 gaaatcgtag tacagctgag gctgctctgt gtcattgagg ctccatgaga gaaaggcgac 60 rccgggattc agatcattgg taattccatg tgggaatctc ggcgttttct ggctgtgaga 120 a 121 <210> 33 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 33 ctctgaaaat tcaccatcat cggctgcttt ggat gtaact gtgaagttgg tactcacacc 60 rgctaaaacc ttgagtagac cttggaagag agcaaaaaga tgcgacgaaa cgcctccgat 120 a 121 <210> 34 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 34 ctgctcgttc ctccaccagt cgtcgatccc gacaccgccc cactgcatct cgaggatacc 60 sgtcgcagca attgaaatga agagtgccat gaaaataaga ctagcatagt tgctaatctg 120 c 121 <210> 35 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 35 atgtccatcc cttttacctt attgtactcg agtgacgaga aaaaaacagt aaaaaaaagg 60 racttttgct tcatagcttt tctcgtggtggtg <212> DNA 211 acct 213> CUCURBITA MOSCHATA <400> 36 cgtcggctcc gtcagtacgg cgaagggagg aaaggagtca tcggcgtttg atgtggaggg 60 raatgcaatt cgaagggagg tggaatcatt gccggagatg gaaccggcgg gggtcgggga 120 a 121 <210> 37 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 37 cagcatttgc cctccacctg ctgctttttt aggcccaaaa tgtgcgcttt gggattgccc 60 rcgacctgcc caagggatgg attggtgtgg ggactactgt agcagttttc atactgcctt 120 a 121 <210> 38 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 38 acctccacca accgggcagg tgatgagggg accgccaccc ccacccagac ctggaatgcc 60 wgcgcccccg cctcgaccgg ggatgcctcc tccacccggt ggggcagttc ctatttacgg 120 a 121 <210> 39 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 39 tttcattttt acttccaatc ttaatcttct tcccttccgc attatcatca tcaactgagc 60 mattcccagt ttgtgaatct cttagattgc tcgtacttaa tgccacttgc aattctgaat 120 c 121 <210> 40 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 40 aagccattga ttacttgaag tccttaaaac atgaagcaag cgttctgggt gtgagctgct 60 wttagatgac ctgcccctaa tttagat 121 tggttaa 41 tgg tttaggt 121 < ggt 212> DNA <213> CUCURBITA MOSCHATA <400> 41 ggagattttg cgtatacttt agatgttgaa gatattgatg aggactcggc tctaaagttt 60 racctgacac ccgtttatcg ggcatatcac attcatg400a gcct ggtggaggcg agcgaggggg cggtgtttgg agcgatggaa ggcgagttcc 60 ygtaaacaga ggacgctgag gctgcgatgt aatggactca atatggcgcg cagaggctta 120 c 121 <210> 43 <211> 121 < 212> DNA <213> CUCURBITA MOSCHATA <400> 43 gatcccaaac aagcgacagc taggagccac ctcggtttt ctcccctctt ccccaaactc 60 rtttatttgg tcactcattg ggtttcgatgt gcgtggagtt 121 210 TA 44UCATA 121 t TA 44 acct ttggcagaat caagctgtgg tgtatccgaa cgaaaagcca aaacaaaact 60 ygattgctgt ctttcaccac gatttggatt ccaagatata cttgcagaca agcacgctgc 120 t 121 <210> 45 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 45 ttatgggctt ggctctgtat cgttggttgg ggcaccataa cagattgata tgaatggtta 60 ktgggagcag gatgcggctg catagacatg aaaacattag gatctgaacc aatggccaac 120 g 121 <210> 46 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 46 tccaacaaca ttcattccaa caaccaccct tccctgctgc tgtaagctcg ataaagatag 60 yggatcgtag ccatttcttc tatga 121 < 212> g DNA <213> CUCURBITA MOSCHATA <400> 47 ctattataga aggattttcc agattctttc agataaagtg aatagaagag aacgataaaa 60 yaaatatgca attgagatgt ggaagaaaga acttgatgat taccggagcg a gtgtggttc 120 t 121 <210> 48 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 48 caaacccggt tcgcagcttg caatcgtttc gctcggaagg aaggaggcaa tcgcacaact 60 yccttggtg 121 < gt acata t 49c tggcaattg 121 gt acaata 212> DNA <213> CUCURBITA MOSCHATA <400> 49 ctggccgttc gtcacgatcg cgctcaaaat actctcaata cgctccacca gttcctcatc 60 racgccctcc gctgcctcca atacgccatc aatcgactgg agaaactcca 213 tag 121> MOSCHATA 50 211 g <212> MOSCHATA 50 211 g gggcatttct caataaaagg tgcccaaaag gctgcacaat tagctgctca 60 rtgccttagc cgagatcaaa agatcagacc tctaatgagt gaagttgtag aagccttgaa 120 g 121 <210> 51 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 51 ggaatcattt cctccccttt ctgctaccca ttcccaacgc ctacttcaat aaacatattg 60 scaccatctt tgtgtcagtg gatttcaaat gctgctagac gtgccactct ctaaccaagc 120 t 121 <210> 52 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 52 ttaaccaagg aggcctcaag ttcagaactt gctgcacaat aaactaccaa ctcaacactt 60 wcattccata aacaa tgttt taagtacagg aggaggcaac ccaagaagct tgtcgtaatg 120 a 121 <210> 53 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 53 cagcgagaag gtgagagagg ggaatgagag agagagctgt gaagaggagctgt gagat 54 cc tggagagt tgatag 121 c 211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 54 ggcttggagt gttcggtggt gttcattgac ttggattgtc gtcttgacat ctcaaggctt 60 kcccaagttt tgaagcttcg aattttggaa ggaaatggt 121 g 400> 55 atgatatgga agatacaagt ttgatgatgt aacattaacg ttgttttgga atcatggcct 60 raagaaggcc cataaagcag caatgtatga taagtagaat attgatgctg caatgtctct 120 t 121 <210> 56 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 56 gcgaatgagc tagtgcagaa gtttcatata gcaatggaga ctgtttctga aaacattaat 60 wgcagtgaag ctggagataa atttacccag cagcataaag aacttctgtc tgttctcgat 120 c 121 <210> 57 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 57 cctgacctcg ccactagcac aaagcatctc tagatatttt g ctcttagtg ggtcccgcaa 60 ragcaacggc caattggagt gcagctcgag atatggacag aatacggatc tttcgacttc 120 c 121 <210> 58 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 58 atacaaatta tgcaattatg ttacacattc cagttgctaa tttccattac cacaattggt 60 waagtatcat tgttcttgtt tgttatcttg ctgcagctgc tcctacatgc tatcgataca 120 c 121 < 210> 59 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 59 tttaagatgc tttgttgggt cactgaagtc aaacaaaacg cagcatagat ttagcttctt 60 racaaacaaa tttctgttttt cagagctt 212 121 < 210 DNA cata 60 aaccttttt cagagctt <gg aacttccc> > CUCURBITA MOSCHATA <400> 60 ggtggaagat aataaggcag ccactcaagt taagcccaac atgggaagac acgtggacgg 60 mggagattgg tcaagaaagc caataacatt atggagtcaa aattctggca aagaagaaaa 120 g 121 <210> 61 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 61 caaatacaag aacgggtggt cggcaatgaa gttgatttga tctaaagcca aggaatgtcc 60 yattacaata gctgtagaag ctgcagcttc tgtgccttct tcattaacct caataaacgc 120 c 121 <210> 62 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 62 caaga atcac tccagaaatc tctcttgcat tgttgcttcg atctttcttt attttctcat 60 raaggtttct taggcatgct tctccagcca gtaactctgc cagtggatca tggatcatgt 120 g 121 <210> 63 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 63 aagcgccttt aacaagttgc ttaaccgcct cgcagctgaa ggccacgatc tttcagctcc 60 mgttgatctt aagaataact gggcaaagca tggaacaaac cgttacatca ccataaagta 120 g 121 <210> 64 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 64 ctaagaagaa agtgagctac aaggacgttg ccagtgaact gcttaaggat tggtaaaacg 60 ygtgttggta tttacttaagaat ttgtgtttata 211 g <gtttgg 65 > DNA <213> CUCURBITA MOSCHATA <400> 65 ttatgagtcg gtatgattct gctagccaga gtcacttttc acttctcttt agatcagtgc 60 wgcagacaat aatgttctag tcattagtag ggtagaaact gatgtctcca attt t aactgatgaa cactaaggag gtgactattg gtgtatgcta 60 mtgttagact acataagtaa tatggaactt catgaccgtg aagctgctag tatctggtag 120 t 121 <210> 67 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 67 ttctcttgga tgaaaactgg gttgcaaagg tttccgattt cgggctctcg aaaactggtc 60 yggatatggc tcatggtcac gttagcacgg tagttaaggg cagctttggc tacttagatc 120 c 121 <210> 68 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 68 cgtgcaagga gatttggcca ctacggaagc agctgaagct gaagcgagcg aggaagccat 60 ygggtattag ctgaaaagaa gcgaatctga ttcgagaggg caagtgaaat tgattcgccg 120 g 121 <210> 69 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 69 atcagaatga aaatagtagt ctccccaaag acgagatgca aacttatcag catcaaaagg 60 rattccatga agtttgacat ataactttgc aaatgattgc aaagtaaatg accatcctgc 120 a 121 < 210> 70 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 70 gctcccggct gccaacatcg acgctacact cttcacccat ctcttcgctg gctacgctca 60 rctcaactcc ggcacttacc aagtcaccgt tgatccttca cagctcatcc tcatcgatca 120 g 121 <210> 71 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 71 gggtacaagt ttgaaaagag aaataattga agttttctgt ggtcattggc agaggcattg 60 stactgcgac agatgagact gatatgattt ctcacattgt ctgccatttt ttctatttga 120 t 121 <210> 72 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 72 tttgaagttt gaatatttca tggattgtag gatggtgcag caggaaagga gtggcggcgg 60 rtgtggggaa gatc gaggtgc 21273 c 121 <agggaa <21273> > DNA <213> CUCURBITA MOSCHATA <400> 73 atagcagtag cctagttagt attgttggac accacaggtg ccactagtcc caaatgttat 60 yacgcctaca gagtacccaa ggaaaagtaa aacctaccac tcta 210 t gg t c t t c t c t c DNAttttt gaatgacaat <120 g C 121 74 212 DNA tcaat SCH 211 g 121 ggaagagctg caggctacgt tggacgaatt acctgcgccc 60 ygacctcaag catggccaat tttcggatat ggaagagcaa actattgtca agctacattc 120 t 121 <210> 75 <211> 121 <212 > DNA <213> CUCURBITA MOSCHATA <400> 75 ttcattcctt tgatatgtct agaatctgat gtcggagtgt acttgcttat cgctaatact 60 ktgacttgaa attcagctgc tgttagtgag aagtcttcga atggttactt accgtaagca agaattcaag ttttacctcc aaatcttatc 60 mgatatcgaa tttctttgat tatgcacgtt ccttaggtcg tttctcttcg caaccttctt 120 t 121 <210> 77 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 77 cagaagtgct tcagtaaatc ttggctagcc agggttgaaa cgtggttctt caccccgatc 60 rtaatcgacc ccaagagaaa cgtgcctcat catctgctcg cagcttacag ccatccttca 120 a 121 <210> 78 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 78 tgtttctctc tcaatcttct catctcccaa gctctcatct cgttcttttg ccaattcctt 60 yatctgtcgt tctctgaatc ccgctagctagcaa <121> 121> DNA <400> 213> CUCURBITA MOSCHATA <400> 79 gtgattcgtc gtgagaacga tgatcctctc atcgccgcag ctcgaccaca atccatccat 60 raaattaagc ataccagaca gcgtcaactg caattcatca cacaaatttc aatttcgatt 120 a 121 <210> 80 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 80 tttgatgact ttgctggctt cttgagttac tgggtcgaca agatagtcag gcacgtcacg 60 yagatgagga gcagggggct tcttgctgag aatcttatca tgtttcaaca aatctgccca 120 a 121 <210> 81 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 81 ggagataaat ttctgaatag gagtgtcaat cttcttagat ttggataaag atgtaagcca 60 ycggcagcag ggtgaaccag tggcaagatg ctgggttgga ggtaaactta aattgagcta 120 c 121 <210 > 82 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 82 caacagtctc ctcttgaatt gtactgaacg acctattcca tgcggaataa tagccaattt 60 sgaccagaat cagccgcaga actgcggctt taa tggcaat aatttgagct gcttcaccga 120 g 121 <210> 83 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 83 cccaccgagt aaacaaagaa caatgagtga cagaccaatc cactggtggc cttagcagct 60 gtagggagcc gcca ctc 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 84 gcctcatcgg ttgtgcaaac ctctgagctg tgttgcaatg tctctggatg gccgctttgt 60 kgctgctgga gaggtggttt tttttttttt ttcctttttg accgtttgtt tttgtggttt 120 t 121 <210> 85 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 85 actatgtcgt cacacttttc acaaaatgaa agctatcaag gtgaaagaga ctcaaaaaga 60 ktaaagctaa caaaagaagg aaatgatgac agttttcctg ttgcaagtga atcgcaggag 120 c 121 <210> 86 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 86 atcccagttg gctgctgttg acatatttgt cagtactgtc gaccctctga aagagcctcc 60 scttgtcaca gctaatacgg tcctatcaat tctcgctgtg gactatccag tcgataaagt 120 t 121 <210> 87 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 87 aaaattcata gtgttggcta gttgaaccta gtttgatttt ttaattctgg tgttaggttc 60 rggcacatca ttcctctatc agtggcctaa tacctatatt aactctctgt tagttagctg 120 a 121 <210> 88 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 88 ttcttatact catgtagctg cactgaggaa ggagatctca gacgagactg atagagagac 60 mgggcgcagt aaacaaattt caagtgttcc aatccatctg agtatctact ctcccaatgg 120 t 121 <210> 89 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 89 gtgattgctt cggttaatcc tccgtacaca atgctcttca ctatctcgaa acaacgccta 60 sgctcggggc gatttgatac aaaggtcata tacacatt 213 c MOSCHATA <400> 90 tgcagctata aattgatctg ttcttttctg ccagaaagtg cttttattta acactagagt 60 kgttgattct attataaaac ttcaggtcta cattaaagca actggagaaa aaagtgctga 120 g 121 <210> 91 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 91 ctatttcttt gttattctcc tttgcagcat cagcctttgc agctagttat tatccatatt 60 ytcttcatct tcttttcttt aaaagttcta ctttgagagg ttcataaacg atataaggct 120 t 121 <210> 92 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 92 acacgaaatt gcgtggctga agt cgtcttc aacctgcagc caatatccag agcatgatca 60 ragctgaaat ggctatggtt atccagctta gaaaggctaa aacaacggag atttgaaact 120 t 121 <210> 93 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 93 gggcatggtg gacgacgtca ctggcaatcc gctgctgtga ttccacttca tggccggttc 60 ratatgctgt ctttgaccgg ttcttttctt cccggtcgag tccctcccgg ttcgagcggg 120 c 121 <210> 94 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 94 tttcaaggcc ctccatttca aatttgagaa gactgttctc ttaaaatgcg atttttgtgcc 60 wtacatttta c caggatctcg gtctcatagg> 212 atc catagg 121 agcatagtg t <213> CUCURBITA MOSCHATA <400> 95 ggcgaaactt agagatatcc cgaggagttc gatttcgtac tctgagttca ctcggatttg 60 yattgatgat tgtggaaaca aggatgatgg tgctgaattc gccaagttgt tggataaatc 120 t 121 <210> 96 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 96 tgactttgat gagtctagct gtaggcttaa tggtcgttgc acttgttaca aaggatctaa 60 ygaggaacag aaatgctgca acttgagcca tcacagaatt tcttgtgcag ctacggttag 120 g 121 <210> 97 <211> 121 <212> DNA <213> CUCURBITA MOS CHATA <400> 97 cgattgcctg gctgcaacta cctgtgaaat ttgaagagca ggcaacaaat gaacaataaa 60 ygtaaatttg ataaaatttc tcgtcctaaa ccctaaacaa acaacaataa taaaggaaga 120 g 121 <210> 98 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 98 gattgaatga agaattccca ctggagagtg tttcttctct gcttccttca atcgtatcca 60 rtcaccagct gctaacccga aactcacccg ctcaagcgta gaagcacaca ctcttacagg 120 a 121 <210> 99 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 99 gtatcagatc ccaccagagg tttttcgacctg aacagggctg caggtaggtgtg g 60t ggtgacctg aacagggctg caggtaggtgtg g 100 211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 100 tataaatccc tttaccccta agttttgggt actgacctct tgctgcatga tgatcatcct 60 rgtcggttgt tgcgaacaaa aggcacgaga atggt 213 BI 121 atgcaacaaa 120 aggtgac 210 atg ttgg 400> 101 agggctgccg aggcatccct cattccgccc agcttcttcc caatctgggt ctcgccctgt 60 mgagcaaatg cgagagcttg tgcaaaaata tgggcaaaac ctatctaaca gcccctccac 120 t 121 <210 > 102 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 102 cgtatctgag agttatgttt gcttgcagtt gcagggtcga aaaccggctc aaaggacaga 60 rtaaatgctc aactccgatg cccgtaaata agttttc <213 ct> 212caag <400> CUCURBITA MOSCHATA <400> 103 acttggtttt gttgcttgtt ctgttcttct catgggttag ctttaagttg aaggcaggct 60 rtgggggatg tcaaagagag acgaggtgtt tgtactttaa gggaatgttc atatgctgcc 120 t 121 <210> 104 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 104 atatgagaat tttgaaaaca agcgaagtaa gagaggtaaa gatacatttg gaaagtcggg 60 maagaaacgg atcaatggag gtgatgaaga atcggataca ggcaggggag gaatggatat 120 g 121 <210> 105 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 105 gatgaacagg gttggctttt cttaaccact tgaattctgt atctaagtaa tccttactgt 60 ygttttgttt cttgtttaca tctatgattt gtttctttgt ttcaattggt ggaatcttct 120 g 121 <210> 106 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 106 agatgcactg ctacacgact cgccaaacga atcctcgtcg acagattcta atttcagtac 60 mactggcagc aacaaggatg catctcag at gtcttcacag aatcctagag aaatgctttt 120 a 121 <210> 107 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 107 tttttttttt ttttgctgca agcagttgag gatgactgaa ccctcaaagg ttattcacgt 60 ycggaatgtt ggccatgaaa tatcggaggt tcatatctct ctctctctat ccctctcaag 120 t 121 <210> 108 <211 > 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 108 ttcaggtcca gaaacctcct gatttctggg acttgttttt gaatttgaat tgttgctctg 60 ytttttaatc cactgatcaa atgagatctt ttatg 212 SCHATA <caagt UCURBITA 212 SCHATA <400> > 109 gggaaatgct gcatcatcca tcaattggaa agggaatgga accaatggaa acagacagtt 60 ygtggatctg agtacctcca aaggaacttc aagtgtgggt tgtaacacgc tccttgttga 120 t 121 <210> 110 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 110 ttgtataggt gtgggagtat gtgctggtgg gggaaaacaa accatttgct acagtcaatc 60 ygtccagtgc catcatccag tgcaaaagtc acatcagtaa ttcttcctgc tctattgcgc 120 a 121 <210> 111 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 111 tttttcaaga ttgaaatcaa tctcaatctc atggttgtct tctat aggaa gcacggatgc 60 mactcctcca gttgatttgt ttttgtagtc ggatcttgct ttaagcgtgg ctgctccttt 120 c 121 <210> 112 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 112 cagctttgtt tttatgttca tgggcttcct tttactgaac cttttgtggt atactgaacc 60 mtttggctct cttaaaggca agagcaggaa taactgaggt gcatttcttg ccgtttaacc 120 c 121 < 210> 113 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 113 ctctgttatt caaactgatg agaatgacaa cttagcagca tccaagaaga tgtcgagttc 60 rggaaaatct ctgtcgaaaaa ctcgaatact 121 gcc > CUCURBITA MOSCHATA <400> 114 tcggtctcac tgctcgtccc ggcttagaat ctggaatgga gcttcgttgt ttatgagttc 60 kgttcttcct tggattagtg gatgatactt tttcttcatg ctgtggtctt gacacaacct 120 t 121 <210> 115 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 115 catgttaaat ctgatgcatc tccaccagat ctccggcagt cttagtcgcc aaacggccac 60 ygacatagct cattccttcg ctattgctcc acaggattac gagtttagtt gcagcaacag 120 c 121 <210> 116 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 116 ctggaaaact tttcttgaga actatgctga agactggaat gatgttgaag ttatcgttgc 60 ytcactctac ctgaaggatg cagaagaggt aaaaacttat atgattgcat atgccaaaaa 120 a 121 <210> 117 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 117 tttaggagag agggctgttt ttgtggatga atgtttattt tgtagcatct cccccattct 60 ygttcaggtt atttgttttc caatgaagta aatgttcatt gactgctact ataaatgtgg 120 a 121 <210> 118 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 118 aacataggag gagttcagcc cctgcagcaa cccaccaggt cggactgaag atctatccac 60 mgaccagtct 210 atgtcctctacgt agtctaaatt agg <br>121 <212 att > DNA <213> CUCURBITA MOSCHATA <400> 119 attgttaact gtatgtgaga ttcactgttc agcacagaat agaatctatg gaacggaaaa 60 ygattggtat gggattcttc aaattgatca atcagcagac gaaacaatta 213 ta <212> MOSCHATA 120atttata cgatttcgca gcatcagaac cggtcaagta tacatgattg 60 yatattcttt ttccgggttc gaaggaagga aatttctcaa ccaagaaatg tttcttcttc 120 t 121 <210> 121 <211 > 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 121 gcgcccgctt gtacaagttg gtgttatatc accaagatag tactgaatac gtgttatgag 60 ycaagtagcc tcaggcaaag cagtt 122 ATA 213 UC 121 gagtt t > 122 caacttctcc tcgccggctg atgatttctc ttccatttcc gcagctcttc cgatggctcg 60 yaaagcttcg attcagcgtt tcttggagaa gagaaaagac aggtgggttt taattcaatt 120 c 121 <210> 123 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 123 tcaattacta agtatatatc tcttctcaac ttgagcatgt aggtgaaact tatgtttcac 60 racaaatagg gcaataagaa ataggaatca gcacttgagt tgtgatatct atagctgttc 120 c 121 <210> 124 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 124 ccaagaaaac caaaatgaaa taaacaaatt tgaagtcgta atttaaaaaaaaaaa g t ca acataact 121 g 121 <210> <212> DNA <213> CUCURBITA MOSCHATA <400> 125 ggaaagctcc gccgtgatta gggcctctgg gacactccat atgaaaggga aaacgatgaa 60 kccaacgatg gcgaggagag ggccggcagc ttggaca gcc ggctcttcgc cataggggcc 120 g 121 <210> 126 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 126 ccgtcacaca tccactgcac accccatcga accctccgcc gtcaaaccct gagcagttcc 210 gagcagttcc 210 g ggc gtgcct ggt aggc gtacggtagagt agg 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 127 gaatgcccat cgggtcggtt tatggagcgt tatcccagga ggtactgaag acgtgattag 60 raggaggcct tcggattttg ctttcgagttt agacatttt 121 210 SCHTA <400> 128 gaaacctcaa aacaaagaat caaatcgcac agcagcagaa acattttcca gagccagaat 60 maaaaatacc tgacagatat cgcaaagagg agaatcgatt ttgatcggct gaagtaggga 120 g 121 <210> 129 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 129 ctctacactg tacccgaata tcgccggcat tctcctgcat acctttgctg cctcctcgtg 60 sgagaatccc aactcctgca agaatctgaa tttgcctctc agcttctcca cccgagtatt 120 c 121 <210> 130 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 130 ctgcgactag tgatctgtgt cccgacccgg aaagttggaa gtgaaatctc catt tcagga 60 ygttcttccc attgtcttgg aggaatttac aggtaattac tcttctttct ggtgccttct 120 a 121 <210> 131 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 131 catagacagt gacaatgagt ttaagaaatc ggattctcat gggcatatcg tcgacgcgtt 60 yggggcgtca atggtagagc cttggcttgc agcccatgga gacccaacaa ggttttcatt 120 t 121 <210 > 132 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 132 ttggttgtcg taactcgatg gcggggacgg aggagtttcg aactcgaaca tactttcagg 60 maattcagaa acatagtaccct ggaaaacata 133 gttcaatg 121g a CUCURBITA MOSCHATA <400> 133 acaagttcaa atgaagaaca ctctactcac actatctgca accgattgaa cgagttcatc 60 rtaactagga ttgacaagac catccccaaa cggcacaaat ttgtaaggaa cttcatatgg 120 a 121 <210> 134 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 134 gactgggcct tcaacggtat cgttgctacc accgggagcc gcggccccgt cggtggtgtt 60 yccaatcttg ccatcggcgc cgggagcctc ggttttatta gcaccggcct tgggagtttc 120 a 121 <210> 135 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 135 caaactcca t ggatacaaag atccagtgaa gtaaactgat ctaaaacaga aaataaaaga 60 raataccacc agatctgcag caaacacaag ccggaatcga aagcggaaaa aaaaaagaac 120 a 121 <210> 136 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 136 gaatgtttcg tttcttggac atgcagcgag agtaagttct tgtgattttg tttgtagcgt 60 yctcgagaac atactcgaag ttttagttgt cttttgtttt taatcgattc taataaaatc 120 t 121 <210> 137 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 137 gatgcaaggc tctgcactgc agggtggaag gatgggagtg gttagcttag cagctggggc 60 yatgcctgtt gcaacaggat 212 cc aga t121 <t t > DNA <213> CUCURBITA MOSCHATA <400> 138 gaggaagcct ttgattgatg cttacgacac gaaaggaaag acgttagacg acatttcggg 60 ygatatagaa ttgagagatg ttcatttg ttcattttag ctatcctacg <212> 139 ctatcct 121 c UCURBITA 139 ctatcct 121 c agg aggacgacac gaaaggaaag acgttagacg a caacggtcat atttctccac agagattcac caaaatcatc 60 ratggccgtt tgtttcctgt tcttttaaac ccgaacctca ctcgcattta atcgaagccc 120 t 121 <210> 140 <211> 121 <212 > DNA <213> CUCURBITA MOSCHATA <400> 140 cttcccacac ggcggagcca tggactcaat ggatcatggg gtttacagcg ggggtggaag 60 yggagacggg tacggcggga ca acttcctgat ttcgatgat ttcgatgat ttcgatggcg gcc 141 g aactaacggt aacagaaatg tttagaaaag cttaccgatt 60 wgcagcaaat gaagtggaga aagtttctac ctcatgtcca acaggcacga aactccaact 120 g 121 <210> 142 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 142 caccaaaaat aagtatccac atgaagaaaa ctagatcaaa tcaaaatgct aagctacttg 60 yaagtcgtaa gccaaaaaga ttgtaattgt gaagagttaa ttgcagttca aatacttgca 120 g 121 <210> 143 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 143 caccaaagga gcaattcttc tcaaaatttt aaaaggtact aagttggaat cccattctaa 60 staaagattc ttgtggtaca t tgactacaa 121a DNA 211> 212a <212> <213> CUCURBITA MOSCHATA <400> 144 ctatgcttct cttagattct gcaatcattc gaatctggga atgagaactg ctaataacag 60 mtggaaattg agaaagtaca cactactact tacattggag agttca atca acaaattaag 120 a 121 <210> 145 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 145 ttttgttttc taattcgatc tcctctgtct cgtgatacag agcttcgaag acaactcaag 60 ytactcaag 121 <212> gaatactcaag 121 <212 gaatcaag a catatagacg <212> DNA <213> CUCURBITA MOSCHATA <400> 146 cttcattcga ttccgcctcc aaattcatga gagccactta tctcctttac gagccatctc 60 kcaaactgtt cgaacaactc accccacgtc cct147cctgcat tatctccgac <213 c ctccttaaga acggacacaa gctcaatatt tgattcttga cttctcttta gctgcagagc 60 kaaatcagca ttggattctt tttggaactt cagttcatct ttcaattcat ctaagatttt 120 t 121 <210> 148 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 148 tcatcattcg cagcaagctc gagcaagctc gaaaaggcat cttccgtttc aacagtcaag 60 ytattcatgg ttgagtccaa gggcttaagc aatcaaatgt acggaagaaa ttactcaaag 120 a 121 <210> 149 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 149 atcgtatgga ggacattgct aagtgcatgc agtgctcgtg atgttgatgg tggggctcag 60 rttgcagagg aggcaagaaa gagactcctt gagctggaga cgaagagggg cggaaatgtg 120 g 121 <210> 150 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 150 ccatcccaac tcacatactg agctctgttt tcacaggcat ctccaaacac caagcttcca 60 yttatgggtg cttttgtccc acaccacaca tggtcatacc ttacgtggta tccgcagcac 120 a 121 <210> 151 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 151 tgataaactt ggaaacagat ttagaggtta tatgacaaaa aatgattgaa gaaaaacaaa 60 ytttagagtc taattttatg ggtttgaagt gggttttgaagt URGAt 210 212 SCHATA <400> UCURBITA 212 SCHATA 121 gagg <400> 152 gaacgataac acgtagctgc aaaatggagt tcaaatttat catccaaaca tgacaaaaga 60 mccatacaag caagccattt gactaagaga atatgaactg tcttacagaa cagtcccaag 120 a 121 <210> 153 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 153 tggtttttct aggtggacta tggcattagt agctatctat tttgcggtat gtctgttgtg 60 watttggtct atatagctta ctttcaattt agtgcctaaa gttttgtgta gagtgcttcc 120 a 121 <210> 154 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 154 taactttgaa aggtccga t t t agttgcagct ccgaaagcaa ttcgaatcag atttttattc 60 racttttggt ttttcttacg aagtagtcgg gtcgggaggt ttacagtgtt tttaacaaca 120 g 121 <210> 155 <211> 121 <212> DNA attatta tgt t t t gg t t gatt t t t agt acc t t t agtat kgcagcggct gctctggctg tggcttgttc tcctgaatca tcacagatat cattaagaaa 120 a 121 <210> 156 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 156 tccattcatc catttatgta tactcaatct tcctcctcca gacctccctg ctctgatttc 60 ycttcaaata atacaaaccc tcaactcttt ttgtactcag ggactcattc tcaagctgat 120 a 121 <210> 157 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 157 tggaactgag aaccagctaa aattccttaa gcctctcttc ttcttcttct tcgtctgccc 60 rgtttgcgat tgagaatgag aggccttcta <211> 121 UC Aagat tgagat <400> 158 atgcaatccg agtcgaggaa atttgaaatc tctatttcca gcaggtcaga acctcagtta 60 yattacatca actcaaactg taattttaac ttgatcactt cttgatattg aaatggctgc 120 t 121 <210> 159 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 159 attatcttat ctaatgctta ttatttgcac agcctcaaaa acttcatttg ataatttctt 60 rcccaaaact gttacccgca tagttttgaa acttcagtta tctccatctc tccctcctgt 120 t 121 <210> 160 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 160 atatgtcgca ttagcaaa ag tagcagcaat taccataatg ggtccagcag ctacaagcga 60 sccaacaacg ctaccgccga caacctgccc ttgaccgccg gacaagtaaa cggtcaggcc 120 a 121 <210> 161 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 161 aggacttgcc ggaaatttgc aggtatatgt gctgattttg aaccaaaaca aaatactctg 60 ktattaacct ctgttttgtg cagagatttt ctggtggatt actcaaaacg ggtgatggag 120 a 121 <210> 162 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 162 gatggtacaa ttcactatcc ttcaattgat tcaagtccga ttgacttgtc atctccaact 60 rcccagacag ttccctttaca t DNA <213> CUCURBITA MOSCHATA <400> 163 cacaaactga atgattgcag ctgaacccag aagttggaga acagtttcca tctgttgaag 60 ragataatct aagacttatt attattggt t ct ctcttgatat agttt t ATA 121 CagUR 210 t t ct t ATA 121 CagUR210 attaacagtc ctctgaaaac cagcccttgc 60 ytctgctgct gccatcaaac tcctatcttt tgaacattca agcagaactt gttaaatttc 120 t 121 <210> 165 <211> 121 <212> DNA <213 > CUCURBITA MOSCHATA <400> 165 taaccgaagg atgattgccg aagcaggagc aataccgttt ctggtgacgc tgttgaaatc 60 yggtgatcca aggatcgaag aaaatgcagt gactgcattg ttcaaccttg caatcttcaa 120 c 121 <210> 166 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 166 aatcataagc gtaaacgcct tttgttaatg aaggacaagc tgcaggttca actgctcgaa 60 yaacggggct tatttttcca gcaagtttct cacggaggaa cgggaagcta agacctgcaa 120 a 121 <210> 167 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 167 ttcttccttt gctttcattc ggaagtcatc ggattgactg tgtagtctac tgaaagtctg 60 kgaatgggaa cgttcacttt tgggatcaga tgtcaagaga ggcgaaatag gtgactcagg 120 g 121 <210> 168 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 168 aaatggctgc tcaagaggac tgaaacctac caccagtctt ggaggttcga aattcttccc 60 yaagccttcc cttaaaaagca tcagtaaaaa 212 a MOSCHATA <400> 169 aaggaagaag atgtctcggg ctcaagattg catcctgaaa tatatgttga aaatgatgga 60 rgtctgtaag gctcaaggat ttgtgtatgg gatcattcct gagaaaggaa aacca gtaag 120 c 121 <210> 170 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 170 accggcgaca ctcggcttca agcttcgcca ttttctttat gctctcaaga tgttgcttac 60 wggctgtttc agctgtactttt gtc taaaa <213> 212> DNA <213> CUCURBITA MOSCHATA <400> 171 acatttctta cccgaaattg cagcatttaa ggccggtctt cttctgctcc cacaacagtt 60 kacacccgcc gtagatatcg atgcggcggc aacggtagac ATA 120 g SCH 172 MOSCH 172211 ctggtgatgc <atggt 121 <ggt> DNA <213> acattctctg tctcccaagc cactactatg aaccgacaca ttggaaaatc 60 mtttcaaatt ccggcagcct ccctcactgc cttcttcgtt gctagcatcc tcgtaaccgt 120 c 121 <210> 173 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 173 gcttatttat ttgcgacaaa gggtctaatt ggcaaacact gcaaccactg caagcacctt 60 yggcgtgtca atggcgaagc tgtatgcgtc gtgtgcagca atggtggccg tgcaaattgg 120 g 121 <210> 174 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 174 ggtgtaagat agattgtggt tctgtaagac catgcctcct gtatcaagct tcctttgaac 60 wgttctgtga tgctgccatt tctttcatcc tccatgctct ccatgtcctt tgtttaatgg 120 c 121 <210> 175 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 175 tcgagctgct tgttctcgca agcgatggac tttgggacgt ggtacgcaac gaggtatgct 60 sactacatca ttaccccatg tatctaagat gctctaaatt gtgaactcca catacgaact 120 t 121 <210> 176 < 211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 176 ggattaacaa aggaggcagt ttgatgaata atgtgacatc ctaagagcag cgaaacgcaa 60 sttccttacc tagtgagcat tttcagtaca cagccattgg tcatatccat <211> MOSCH121 177 400> 177 tgatcattgt aaatggaaaa ttacagagaa atttcaaaaa cggaattata gcaacgactt 60 rtaccagata ctggagcatt agctttccca gcataaaagg tcttggccct gacgacatca 120 a 121 <210> 178 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 178 tccaccagca tccgacgact tggcgtacag gtgctcattg aggcataaaa acaaacacct 60 watgggcaca agctctgaac taatttcatg gtccccaaag cacgatgctg cagttgtatc 120 t 121 <210> 179 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 179 atcatcttcg gccgaaaact cctctgt ttc gtcgcagacg atatcggtgt cgtcaaattc 60 mccgctgcca agctgacaat gggggagtca ttagtaatgt cgataagcgc cgatcgatct 120 t 121 <210> 180 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 180 tatgatattc atgagtgttt gaatcaacgg gtttgatatt gagttaccat tttgatgaat 60 kcccataagg tatttgatga aattcccagc tgccattttt agttttttgt gcttgaaaaa 120 t 121 <210> 181 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 181 actatggtcg tgacaagtcc caattcagta tactaatgaa tgatttctta ctggtgagtt 60 ragcaaattt gtttgcatacta agcca tt 121 tt 182 c <213> CUCURBITA MOSCHATA <400> 182 aattattcag cgttgactaa ttgtattgtc cccaagtaat gataaatgca tgcgagcaaa 60 ratatgaaag agtatttgtg tatcacagaa tctggactcg atataaggga tgagagcaaa 120 t 121 <210> 183 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 183 tgacgttttc actgcagatt tcaattccag taaaaaccag tgccaatttt cctccgtctc 60 sgcatctaca actgcaaaag ctgctggaaa tatgccatct tccccatcca cagctgtggc 120 a 121 <210> 184 <211> 121 <212> DNA <213> CUCURBIT A MOSCHATA <400> 184 tgactgtatc tctatagtca ataattcttc aaggatcttg atcaaagaca ttgcctgtgg 60 rccaggccat ggaataaggt aacataaatc ttcactagtt agatcactcc gattggatca 120 a 121 <210> 185 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 185 ggtttagttt agcttttttg attaagatcc tgttagtttt gtttgtgtta attgaaggat 60 kcttgagaac ttctcctcca ggctgatttc ctgtagctgc catgttcttc gttgttcaat 120 t 121 <210> 186 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 186 caattaagac ctcgaaactc caaactccag atcggttcca gagatgaaaa ctctatacaa 60 racgaactgg taagaggaag aagctcggag cttcttcaat ggcggtttct agggctttag 120 g 121 <210> 187 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 187 attaaattca cattcgagag gagtggaagc agccgtagga ttgaagcaag ggtaggatag 60 kaaattaaga caaaagtgaa gggcagg 121 CUCURBITA cagtcgcacg 211 210 TA 212UC <400> <400> 188 aatataattt gctttagaac ataaagcagc agaagaaaga aaacacgaaa atcaatcaac 60 yaacaactcc cagatatttc tagaattgta aggaattaga ccaaccggat gggatgatct 120 c 121 <210> 189 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 189 atacaggttg atcactctac aaaaggtgaa ttaccagcca aacgtgtcag tgcaagaatg 60 raagaaaatg tgaacttgg ttttctt 211 ctt at gt t t DNA <213> CUCURBITA MOSCHATA <400> 190 atatttggca tagacgaagg tctttagttg gatgggcagt tgtctccaac tcacaggctt 60 ycatcgagtg cccatcagtc ctctgatttg aggtgaattc tgatgcagtt gtgatcattg 120 a 121 <210> 191 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 191 agattcagat ttacaggtga tgaaggagaa attaaaccct gcaatcttcc agggaaacgg 60 rgaggttgaa ggtaagaaaa tcagtgggag aagtaacggg gaaagtttct cggtgtcgaa 120 g 121 <210> 192 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 192 gttcgaaatc gtaatcgccg tcgacccatg aatcgcatcg atcaacccat cattacagtt 60 sgacaacgag cagtgatcca cccagacatg gctgccgccg aagattgaaa cgccgtcgcc 120 g 121 <210> 193 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 193 cattagcaat gtctatagcc cccgtcgtcg acagagacga tatcggctgc gtgaggagct 60 yaaacaacca aacctacta t cacaatcttg ctctcagggc ctcattgcaa tctttaagga 120 g 121 <210> 194 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 194 ttgcgagggc atctggacca gcagcagctg cttcctcggc cttctgtttc tcttcctcga 60 stttgaactt cggcttccat ttggtgtcgc ggtatgaact agcgagtttc tcgtctttgg 120 c 121 <210> 195 < 211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 195 acagatgccc ttcttcattg acatttgtca gtctgtagaa agatagccaa gcatgacagc 60 yttggataaa tttggtgact aaactgtata aaat 213 TASCHATA <400> 196 cc ATA 213 TASCHATA <400> 400> 196 catctcagaa tacaccgttg ctcagtataa ttcgtcacgt cacctgtcgg gtcgtttgaa 60 ygaagtctgg atcgacgttt cagctgttac atcagagggc tgtcaacgcc ggtaacgagt 120 t 121 <210> 197 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 197 ctctgacatt tttcttctca gctgcggctc atcctccagt cccaccttct ttaaccgcac 60 yttcgtcggc gattcagtca aacccgcctc cgactttctc actgccggcc gatcggtggc 120 g 121 <210> 198 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 198 gtggtgggtt ttgcaaaact cctcgagctg ctggac ctgg gagaagttct gaaagtgagg 60 raagaaaacc ccaatatcct cgaacatttt gagtgcccaa tgaaatcgaa atctcttaaa 120 a 121 <210> 199 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 199 tcggtactta ctgctgcaga caattgattg catgattccg gaaggctccc agatggatta 60 maaaattgac ctgccaagaa caattagcaa gattatttta acaagatatg tcatgggcgc 120 g 121 <210> 200 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 200 tcgtagagct acaagaacga agatgatgat tcaatgtttt ttgacatttg gcttctgaac 60 ktttaaatgc ttgctctgtc ttttgctgtc ttttggttt 201 <gtt g 213> CUCURBITA MOSCHATA <400> 201 atcaaaccgg atctccaagt gatagctaga gagaaaagat tgatatctaa tactcattgg 60 watcgtggca cgaagaactt ggatcggaca aggcaagatc atcgttggta acctagaatc 120 t 121 <210> 202 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 202 aaaaacacta gctggtttgt tgccatcgag aatcggtttc ccgaaacgat cggtaacatc 60 sgcggttgtc atgatggctg atttgatagc tgccggtgtc cattttggat gagctgaatg 120 g 121 <210> 203 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 203 ggtcgactct gtctcgactc tcggcttcaa tcgattggtc cgatcctact acgaggactg 60 yggcatctgc tgatgctgct atcttctttg cttcatctag ctgggcagag ctgcaagcca 120 c 121 <210> 204 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 204 ttgatttgat gtttgtgtct aaaaatgcag ctttccctcg agggagcaat ggtccatcta 60 rcctctcgtt acaagctagt caaaacgaaa agactgaatc tcatgcacaa caagatcaaa 120 a 121 <210> 205 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 205 tcaaagactg caccaaatga gtctgaattc tccattgaag attgatggtc tttttgcacat 60 wgtgagtttttgc agcacaattt tgc ta > DNA <213> CUCURBITA MOSCHATA <400> 206 cacccaagtc ggccccgtct gccggagctt gatcgtaaaa aagattctcg acgtttcggg 60 kaactgcatt ctcgggctgc cggURaccagaa ATA 120 gatgataaaa gaaacaaccc aaagagattc cccattcaac 60 raaaggcagc tgtgttcttg tgtggatacc caccacaagc tccttacttg taaataccaa 120 a 121 <210> 208 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 208 ctatgttgca aatctaggtg attcacgtgc tgttttgggt agagtagtga aggcgactgg 60 sgaggttctc gccgttcagt tatcgacaga gcacaatgt> 121 TA 213 tctaggca 209 gcactctcga ccgagcccta aataccgtga acgaaagcaa caaagcttcg ccgattcggt 60 kctgcagcga ttggaggata agtgatgtca gccagtacct agtcttgaaa ctgcttttac 120 c 121 <210> 210 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 210 tatatacact catccatcat atacactcat ccatcgccct ctcgctctct ctcgtgcgtt 60 ktgtatgtac aggaagattg taacagtgga agagatctta acaattgaca taaaaccagg 120 t 121 <210> 211 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 211 gttggagcag ctctttgaca gctccctcgt gggttgagta ttactcatct atttgtagct 60 ytccacttat caatggatat tc ttat 211 <400> > DNA <213> CUCURBITA MOSCHATA <400> 212 agttttagcc ctaaagaaag ttgtaatttc aaagaaatac cctcaacgaa cggctgggac 60 sctctccagg gaagtgagct acctgacctg aaccttttt ggcgtgta gtacaacatg atcggaagaa caatctaaaa atcggcagca 60 rcaacaacaa caacaacaac aacaacatca tcataatgcc gcctagcgtt acgaagaatc 120 a 121 <210> 214 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 214 acagaatcaa acagtctcta tgagacttat aatcaccact catatggttc ttcaccggcc 60 sattcgttct tcgatggagt ttcatccgctcgcca gatttctctcca <ctccaaaaca> 121 a CUR210gcca gatttctcca 215 ccaagttctt gacgattcaa ccgaccctgt cattaaggta ctcaaaaact cttctaatct 60 yccgactctc atcgttttca atccccaaat cacaaaaacc tgatttccca acacgaaacc 120 c 121 <210> 216 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 216 aaacatcctc ttgaacagaa cttaattatg tttggcaatt gtgaaaactg aatgttgtgt 60 kgttattttg tttgaaatga ggacagaatc aacacattgg agctgggatt tgcagcgttc 120 c 121 <210> 217 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 217 ttcataataa taaagaaaaa ctcagtctat ttgattccta aagaccaaga ctgcaaaaat 60 mgcaacaagccat tcagctata 121 c 212> DNA <213> CUCURBITA MOSCHATA <400> 218 ttatttataa tgcgggacat tatctcctgc agccggaagc ttggacatgg atcgatcaat 60 ycattgataa gagtctaaag atctttggca atggtgtg aa tacccgattt cgggaggatc 120 g 121 <210> 219 <211> 121 <212> DNA <213> CUCURBITA MOSCHATA <400> 219 tcatttactg gtgatcgatc atcattgata tttgaacagc acgtacctgg agagagtgtc 60 ratgagctga aaaagaaaat tcagtcacag gtgattttaa catgcttcaa tttgtgttat 120c 121

Claims (6)

In the nucleotide sequence of SEQ ID NOs: 1 to 219, a composition for identifying trait differences between varieties of oriental pumpkin, comprising an agent for detecting all single nucleotide polymorphism (SNP) located at the 61st nucleotide of each nucleotide sequence.
The method of claim 1,
The formulation contains a polynucleotide consisting of 5 to 121 consecutive bases comprising a single nucleotide polymorphism site located at the 61st base of each base sequence in the base sequence of SEQ ID NOs: 1 to 219, or a nucleotide sequence complementary thereto. A composition for identifying trait differences between varieties of pumpkin.
The method of claim 1,
Wherein the agent is any one of a probe, a primer, and a combination thereof, a composition for identifying trait differences between varieties of oriental pumpkin.
A kit for identifying trait differences between varieties of oriental pumpkin, comprising the composition of claim 1 .
5. The kit of claim 4, wherein the kit is a PCR kit, microarray, or kit for Fluidime SNP analysis.
a) isolating a polynucleotide from a repeating oriental amber sample, and determining the genotype of all single nucleotide polymorphisms present at position 61 of each nucleotide sequence shown in SEQ ID NOs: 1 to 219;
b) isolating a polynucleotide from a backcross progeny oriental amber sample, and determining the genotype of the single nucleotide polymorphism in step a);
c) comparing the single nucleotide polymorphism genotype of the repeat parent determined in step a) with the single nucleotide long polymorphism genotype of the backcross progeny determined in step b); and
d) A method for selecting a backcross progeny of oriental amber, comprising the step of selecting a backcross progeny with a high ratio of matching repeat parent and single nucleotide polymorphism genotype from among the backcross progeny.

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