KR102444281B1 - Soil conditioner composition and uses thereof - Google Patents

Abstract

The present application relates to a composition for soil improvement, a method for promoting plant growth or biomass using the same, and a method for improving soil, using the composition for soil improvement according to an example to promote plant growth or biomass with excellent efficiency It can improve the mass or improve the soil, especially in semi-arid and/or arid regions for preventing desertification, promote excellent plant growth, promote biomass, improve soil, and/or inhibit soil erosion through soil surface fixation there is

Description

Soil conditioner composition and uses thereof

The present application relates to a composition for improving soil, a method for enhancing biomass using the same, and a method for improving soil.

Soil conditioner (or soil conditioner) refers to a substance used to improve the physical, chemical, and/or biological properties of soil in a soil with an impact on plant growth/crop quality and safety, usually under natural/human influence. do. Unlike fertilizer, the exact composition and chemical composition of soil reforming vary depending on the source, and soil permeability and water retention characteristics can be improved by using a soil preparation. Impaired soils are mainly poorly vegetated, poorly structured, hindered plant root growth, low fertility, or lack of nutrients (unbalanced, acidified, salinity, excessive or insufficient soil moisture, toxic substances, etc.). Obstacle soils include sandy soil, clay soil, structural obstacle soil, acid soil, saline soil, alkaline soil, and contaminated soil ( Contaminated soil), etc.

Depending on the nature of the soil with disabilities, add organic matter or fertilizer containing a large amount of organic matter, add a pH adjuster, add lime, such as quicklime or slaked lime, or include inorganic salts to maintain moisture in the soil, , a substance containing a water-soluble polymer as a main component is added to improve the moisture content of the soil.

Sandy soils are mainly found in dry areas and appear in soils undergoing desertification. In dry areas, the soil is eroded by wind or the soil is eroded by concentrated rainfall, so the survival rate of planted water is low. It is difficult to form a forest or grassland because the roots of plants such as trees or grass cannot use not only water but also nutrients.

In dry areas, various soil improvement attempts have been made by adding hygroscopic polymers such as organic/inorganic fertilizer fertilization and acrylic sodium salt polymer (ASAP) or polyacrylamide to maintain moisture in the soil to prevent desertification. have. However, organic matter, including fertilizer, is difficult to maintain or improve moisture in the soil. In particular, when fertilizer is used excessively to increase crop production in dry soil, it rather deteriorates the soil or causes water pollution in rivers and rivers. .

Inorganic salts such as MgCl ₂ can retain moisture in the soil, but in particular, in sandy soils, they are easily dissolved by rain and are easily removed from the soil. . In addition, the use of an excessive amount of inorganic salt may cause environmental problems such as soil acidification and plant death.

The hygroscopic polymer requires a long time to be decomposed in the soil, and the hygroscopic polymer and/or its decomposition in the soil may act as a toxic component in the soil ecosystem, thereby causing environmental pollution.

Therefore, in order to improve soil barriers for the prevention of desertification in dry areas, without using inorganic salts or polymers that cause soil toxicity as main components, improve the survival rate/production of crops during afforestation or grassland formation and agricultural activities in dry areas. To this end, there is a need for a soil preparation agent that maintains/improves moisture in the soil while preventing the outflow of fertilizer components in the soil and enhancing the fertilizer effect.

Republic of Korea Patent Registration No. 10-2056204

An object of the present invention is a composition for improving soil comprising lysine and citric acid, plant growth promotion or bio It is to provide a method of increasing the mass.

Another object of the present invention is to provide a method for improving soil, comprising treating the soil with a composition for soil improvement comprising lysine and citric acid.

Another object of the present invention is to provide a composition for improving soil, comprising lysine and citric acid.

Another object of the present invention is to provide a fertilizer composition comprising the composition for improving soil.

Accordingly, the inventors of the present application have found that the composition for soil amendment or soil conditioner according to an example is more environmentally friendly than the existing composition for soil improvement, does not use the composition for soil improvement, or does not use water or the existing composition for soil improvement ( The present invention was completed by confirming that plant growth promotion, biomass promotion, and/or soil improvement effects (eg, soil desertification prevention) were superior to those using a commercial soil improvement composition).

As used herein, “about” or “approximately” generally refers to within 20%, within 10%, within 5%, within 4%, within 3%, within 2% of a given value or range above and below; It may be interpreted as meaning including a value or range within 1%, or within 0.5%.

As used herein, “soil conditioner”, “soil preparation composition”, “soil conditioner or soil amendment”, “soil conditioner”, or “soil improvement (or improvement) composition” refers to (1) soil particles improving the physical and/or chemical properties of the soil by changing the binding or inter-particle voids, and/or changing the soil pH; (2) absorb, retain, increase, and/or enhance moisture in the soil depending on the cohesive and viscoelastic properties of the composition; (3) inhibit soil erosion; (4) prevent leakage of nutrients into the soil; (5) increase, and/or enhance the effectiveness of fertilizers in the soil; (6) increase, and/or improve the viability and/or biomass of the crop (or plant); And/or (7) refers to a composition for improving (or improving) soil by improving the chemical, physical, and biological conditions of the soil suitable for plant growth.

In general, chemical fertilizers are commonly used for soil improvement, and these chemical fertilizers have the function of supplying crops by temporarily increasing the soil strength. can In particular, the soil conditioner currently used in agriculture is cumbersome to separately fertilize the soil conditioner with different properties for the growth of crops, and may cost a lot of money. In addition, chemical fertilizer application for soil improvement in desertification areas is difficult to maintain moisture due to the characteristics of dry soil in desertification areas, and nutrient leakage and salt accumulation are serious, making it difficult to see the fertilization effect. costs a lot for

As used herein, the term “soil” includes a laminate such as soil or dirt or ground on the surface or underground. For example, soil contains particles or grains of a size that can be eroded by wind or the like.

Hereinafter, the present invention will be described in more detail.

One aspect is a composition for soil improvement comprising lysine and citric acid (soil preparation composition) comprising the step of treating at least one selected from the group consisting of plants, seeds of plants, soil, and plant planting soil, the plant's A method for promoting growth or enhancing biomass is provided.

As used herein, "promoting plant growth" means increasing, enhancing or stimulating plant growth or plant development, improving plant yield (eg, increasing the number of fruits and/or increasing the weight of fruits), or increasing the number of roots. to increase, increase root mass, increase root volume, increase root growth, increase photosynthetic activity of plants, increase the content of pigments (eg chlorophyll) contained in plants, or , increase leaf area, increase plant orthostatic properties (less plant verse/lodging), increase plant height, increase plant vigor, or reduce crop production. increase, increase the dry weight of roots and/or shoots, increase plant biomass, or refer to a combination thereof. "Improving", "increasing", or "enhancing" in relation to plant growth (growth) means that plant growth (growth) is generally one or more properties or parameters in terms of soil improvement composition (soil preparation) according to an example. This means an improvement compared to the untreated control or water, or the existing composition for soil improvement.

As used herein, "biomass" means the dry weight or fresh weight of a plant (crop), and biomass is all parts of a plant, for example, shoot biomass (all plants on the ground), unless otherwise specified. part), leaf biomass, fruit biomass, flower biomass, and root biomass. The "dry weight" refers to the weight of a plant dried to remove moisture from a majority of cells, and the "fresh weight" refers to the weight of a plant that has not been dried to remove moisture from a majority of cells. Biomass can be measured by a biomass measurement method known in the art.

As used herein, "enhancement of biomass" is to increase the dry weight and/or fresh weight of a plant (crop), and may include increasing the dry weight and/or fresh weight of the plant by promoting the growth of the plant. have.

The “lysine” is one of the basic α-amino acids, which is synthesized by itself during the growth stage of plants, but cannot be synthesized in the body of humans or animals, and is an essential amino acid that must be ingested from the outside. Food additives, pharmaceutical raw materials, livestock It is used as a feed additive for the growth and development of

The lysine may be commercially available or produced using an extraction method, a fermentation method, an enzyme method, and/or a synthesis method. For example, it may be obtained by purification after obtaining a fermented product containing lysine using a coryneform strain, or biosynthesized through a lysine biosynthetic pathway from oxaloacetic acid, or chemically synthesized.

The lysine may be at least one selected from L-lysine, D-lysine, DL-lysine, and salts thereof.

The lysine salt may be lysine sulfate, lysine acetate, lysine monohydrochloride, lysine dihydrochloride, lysine monohydrate, lysine acetylsalicylate, lysine phosphate, lysine diphosphate, mixtures thereof, or a combination thereof. The lysine salt can be converted into a lysine free form by a commonly known method. Conversion of the lysine salt to the lysine preform may be performed by a method known to those of ordinary skill in the art.

The “citric acid” is an organic acid found in citrus fruits, which is an intermediate product of the citric acid cycle that occurs in the metabolic process of oxygen-breathing organisms, and is a flavoring, chelating agent, acidic seasoning, and/or synergist. ) and so on.

The citric acid may be commercially available or may be produced by extraction and/or microbial fermentation. For example, extracting citric acid present in the free state in seeds or fruit juice of various plants, or surface fermentation method or liquid fermentation method using the mold Aspergillus niger , or fermentation method by citric acid-producing microorganisms It may be manufactured by

In one example, the composition for soil improvement may further include a solvent.

The solvent may be an aqueous solvent. The aqueous solvent may include, for example, water and/or alcohol, and may include water as a main component and alcohol as an auxiliary component. The solvent may be at least one selected from the group consisting of water (or deionized water), a primary alcohol, a polyhydric alcohol, a diol, and a triol.

In one example, the mixing weight ratio of water (or deionized water) and alcohol included in the composition for soil improvement is 1:1 to 10:0, 1:1 to 10:1, 1:1 to 5:1, or 1: It may be 1 to 3:2. The alcohol solvent may be monohydric alcohol, polyhydric alcohols, unsaturated aliphatic alcohols, alicyclic alcohols, or a mixture thereof. The monohydric alcohol may be, for example, at least one selected from methanol, ethanol, propan-2-ol, butan-1-ol, pentan-1ol, and hexadecan-1-ol. The polyhydric alcohol is, for example, ethane-1,2-diol, propane-1,2-diol, propane-1,2,3- Triol (propane-1,2,3-triol), butane-1,3-diol (butane-1,3-diol), butane-1,2,3,4-tetraol (butane-1,2, 3,4-tetraol), pentane-1,2,3,4,5-pentol (pentane-1,2,3,4,5-pentol), hexane-1,2,3,4,5,6- hexane-1,2,3,4,5,6-hexol, heptane-1,2,3,4,5,6,7-heptane-1,2,3,4,5,6 , 7-heptol) may be one or more selected from the group consisting of. The unsaturated aliphatic alcohol is, for example, pro-2-en-1-ol (Prop-2-ene-1-ol), 3,7-dimethylocta-2,6-dien-1-ol (3,7-Dimethylocta -2,6-dien-1-ol), pro-2-yn-1-ol (Prop-2-yn-1-ol), cyclohexane-1,2,3,4,5,6-hexol ( cyclohexane-1,2,3,4,5,6-hexol), and 2-(2-propyl)-5-methylcyclohexan-1-ol (2-(2-propyl)-5-methyl-cyclohexane- It may be at least one selected from 1-ol). The alicyclic alcohol may be, for example, at least one selected from cyclopentanol, cyclohexanol, and cycloheptanol.

In one example, the composition for soil improvement may be in the form of an aqueous solution further including an aqueous solvent, and lysine and citric acid included in the composition for soil improvement exist in the form of a salt solution in the aqueous solution and precipitate as crystals. or do not form precipitates (or precipitates). In one example, when the composition for soil improvement includes a salt of lysine and citric acid and water, the lysine and citric acid may not form a covalent compound or an insoluble salt.

In one example, the content of the salt of lysine and citric acid included in the composition for soil improvement is 0.1wt% or more, 0.5wt% or more, 1wt% or more, 2.5wt% based on the total weight of organic solids More than, 5wt% or more, 10wt% or more, 20wt% or more, 30wt% or more, 40wt% or more, 50wt% or more, 60wt% or more, 70wt% or more, 80wt% or more, 90wt% or more, 99.9wt%, 100wt% or more, 0.1 to 100 wt%, or 0.1 to 99.9 wt%.

The precipitates (precipitates) may be one or more precipitates selected from lysine and citric acid. The precipitate (or precipitation) is, for example, an insoluble salt (AB(s)) is obtained by chemical change of an aqueous lysine solution (A(aq)) and an aqueous citric acid solution (B(aq)) as shown in Scheme 1 below. As shown in Scheme 2 below, lysine solid (A(s)) or citric acid solid (B(s)) is precipitated from an aqueous lysine solution (A(aq)) or an aqueous citric acid solution (B(aq)), or a lysine solid (A (s)) or the citric acid solid (B(s)) is not dissolved in the solvent and thus remains insoluble. In addition, the composition for soil improvement includes a case in which a thickener is precipitated or the thickener is not dissolved in a solvent and thus remains in an insoluble state.

[Scheme 1]

A(aq)+B(aq)->AB(s)

[Scheme 2]

A(aq)->A(s)

B(aq)->B(s)

The "sediment" is before and after spraying the composition for soil improvement; Before and after the step of treating at least one selected from the group consisting of plants, plant seeds, soil, and plant planting soil; before and after the step of treating the soil; keep; and/or produced during distribution.

In one example, the content of lysine, citric acid, and water in the composition for improving soil may be controlled so that lysine and citric acid are not precipitated as crystals or precipitation is not formed. When the composition for soil improvement maintains a liquid state without forming crystals or precipitation, it is treated with one or more selected from the group consisting of plants, plant seeds, soil, and plant planting soil, promoting plant growth, and promoting biomass , and / or soil improvement (eg, physical and / or chemical soil improvement of the soil by a composition for soil improvement; maintenance and improvement of moisture and nutrients in the soil by the moisture content and / or hygroscopicity of the composition for soil improvement , or increase; increase in soil aggregation due to improvement in soil particle cohesion due to the composition for soil improvement; improvement in soil porosity; and/or maintenance, improvement, or increase of moisture and nutrients (fertilizer components) in the soil) effect can be increased have.

In one example, even if the composition for soil improvement is dried to form crystals, when a solvent (eg, an aqueous solvent) is added to maintain a liquid state again, the group consisting of plants, plant seeds, soil, and plant planting soil Treatment with one or more selected from, promotes plant growth, promotes biomass, and/or improves soil ((eg, physical and/or chemical soil improvement of soil by a composition for soil improvement; composition for soil improvement) maintenance, improvement, or increase of moisture and nutrients in the soil by the moisture content and/or hygroscopicity of the soil; increase in soil aggregation by improving soil particle binding strength due to the composition for soil improvement; improvement in soil porosity; and/or moisture in the soil and nutritional component (fertilizer component) maintenance, improvement, or increase) effect may be increased.

In the present specification, promoting the growth of plants, promoting biomass, and / or improving soil means that the composition for soil improvement according to an example is not treated or when water or the existing composition for soil improvement is treated than when the growth of plants is increased. It means that promotion, enhancement of biomass, and/or soil improvement effect is enhanced, increased, improved, or increased.

Compositions (existing soil improvement composition, commercial soil improvement composition, commercial soil preparation agent) used for existing soil improvement are made of polyacrylic acid (ASAP; acrylic sodium salt polymer) or polyacrylamide to retain moisture in the soil. There is an organic composition made by utilizing a hygroscopic polymer such as, and/or a by-product generated in agriculture and livestock industry, or an inorganic composition such as MgCl and lime.

Hygroscopic polymers have an effect of retaining moisture in the soil, but do not have an effect of increasing biomass, require a long time to be decomposed in the soil, and the hygroscopic polymer and/or its decomposition products in the soil act as toxic components in the soil ecosystem. Because it causes a problem of environmental pollution, repeated use in high concentrations or large amounts is impossible due to biotoxicity and soil toxicity.

Organic composition is difficult to maintain or improve moisture in the soil, and excessive use causes trace elements such as iron, copper, and manganese in the soil to combine with organic matter and become unavailable to plants, resulting in a deficiency of trace elements in the soil, Alternatively, excessive use may affect plant growth due to excessive accumulation of nitroxide in plant cells. In particular, when an organic composition is used excessively to increase the production amount of plants (crops) in dry soil, it rather accelerates soil degradation or causes water pollution in rivers and streams.

Inorganic composition (MgCl ₂ , inorganic salts such as lime, etc.) is easily dissolved by rain in sandy soil, is easily removed from the soil, and flows into rivers, etc. to negatively affect aquatic ecosystems such as rivers by salt concentration. In addition, the use of an excessive amount of the mineral composition as a soil improvement application causes soil environmental problems such as saline soil or soil acidification by changing the soil pH, and when the basicity of the soil increases, phosphorus, zinc, magnesium, calcium, and boron in the soil It can cause growth problems, such as plant death, because the plant cannot use it by hardening nutrients.

In one example, the composition for soil improvement may be in a liquid form. When the composition for soil improvement is maintained in a liquid state, it can be uniformly mixed with the soil or is easy to apply uniformly, so a composition or a composition for improving soil in a solid powder form (or powder form) containing crystals or precipitation of lysine and citric acid It may be more excellent in promoting plant growth, enhancing biomass, and/or improving soil.

In one example, the composition for soil improvement may be one in which no sediment is formed after storage or distribution for 14 days or more. For example, 14 days or more, for example, 12 months or more, for example, 24 months or more, even if stored, the composition is stable and the physical properties can be maintained as it is. In addition, the temperature of the environment in which the composition for soil improvement is stored may be -18°C to 80°C, specifically -18°C to 45°C, 0°C to 60°C, or 20°C to 40°C. Even if stored outside the temperature range, if the temperature of the environment in which the composition for soil improvement is used is within the temperature range, the formulation and quality may not be affected. For example, when stored at a low temperature, it can be used after leaving it at room temperature for a certain period of time before use.

In one embodiment, the composition for improving soil is applied or delivered directly to a plant, plant part (eg, leaf, stem, branch, root, and/or flower), and/or plant seed, or the plant is growing or growing. It may be applied to or blended with soils that are intended to be seeded or that are intended to be sown (eg plant-planting soils). For example, spreading, spraying, drenching, parts of plants (eg, leaves, stems, branches, roots, and/or flowers), soil, seedlings? It can be carried out by any means, such as pouring or soaking the seeds before planting and/or soaking the seeds after planting, and can be used in combination with the various means described above.

In one example, the step of treating the composition for soil improvement on one or more selected from the group consisting of plants, plant seeds, soil, and plant planting soil is immersion method, soil mixing method, soil spraying method, application treatment, fumigation It may be carried out by one or more methods selected from the group consisting of treatment, spraying, irrigation, and indirect absorption.

In one example, the concentration of the composition for soil improvement for treating the composition for soil improvement on one or more selected from the group consisting of plants, plant seeds, soil, and plant planting soil is a plant species, properties and state of the soil to be treated , may be appropriately selected according to the means of application and the stage of plant growth.

In one example, the immersion method may include the step of wetting the roots of the plant in the composition for soil improvement, in order to facilitate the wetting step, 0.1 to 5%, 0.1 to 10 of the composition for soil improvement %, 0.1 to 20%, 0.1 to 30%, 0.1 to 40%, or 0.1 to 50% (wt% or volume%) may be diluted and used. In one example, by treating the composition for soil improvement having a diluent concentration in the above range, the binding force of soil particles in the soil near the plant root, the physical and/or chemical soil improvement, and the wettability of the soil can be further improved.

In one example, the soil mixing method 100 to 4500kg / ha, 300 to 4500kg / ha, 300 to 2400kg / ha, 600 to 900kg / ha, 450 to 4500kg / ha, 600 to 4500kg / ha of the composition for soil improvement , 900 to 4500 kg / ha, 1800 to 4500 kg / ha, 900 to 4000 kg / ha, 900 to 4000 kg / ha, 1200 to 3000 kg / ha, or added at a concentration of 1500 to 3000 kg / ha and mixed with the soil. When the composition for soil improvement is mixed with the soil at a concentration within the above range, the plant growth promotion, biomass enhancement effect, and/or soil improvement effect may be superior to when mixed at a concentration outside the above range.

According to one example, even if the concentration contained in the soil is increased (or the solid content in the composition is increased) unlike the conventionally known composition for soil improvement, the growth of the plant (eg, the viability of the plant, the plant growth height, and / or chlorophyll concentration) and/or to improve the effect of promoting plant growth, promoting biomass, and/or improving soil without negatively affecting the environment of the soil.

In one example, the above soil spraying method is 100 to 4500 kg / ha, 300 to 4500 kg / ha, 300 to 2400 kg / ha, 600 to 900 kg / ha, 450 to 4500 kg / ha, 600 to 4500 kg / ha of the composition for soil improvement , 900 to 4500 kg / ha, 1800 to 4500 kg / ha, 900 to 4000 kg / ha, 900 to 4000 kg / ha, 1200 to 3000 kg / ha, or it may be to spray the soil at a concentration of 1500 to 3000 kg / ha. When the composition for soil improvement is sprayed on the soil at a concentration within the range, the plant growth promotion, biomass enhancement effect, and/or soil improvement effect may be superior than when sprayed at a concentration outside the range.

The indirect absorption method refers to a method used by plants indirectly by injecting them near the roots at a distance that does not directly touch the roots of the plants.

In one example, the irrigation or indirect absorption method is diluted with 0.1 to 5%, 0.1 to 10%, 0.1 to 20%, 0.1 to 30%, 0.1 to 40%, or 0.1 to 50% (% by weight or volume) Can be used. In one example, by treating the composition for soil improvement having a diluent concentration in the above range, the binding force of soil particles in the soil near the plant root, the physical and/or chemical soil improvement, and the wettability of the soil can be further improved.

In one example, soil, seeds, seedlings, or plants (crops) may be treated with the composition for soil improvement as many times as necessary at any stage, and the number of applications may be, for example, a fertilization program, plant species, development at which treatment is initiated. It may be determined according to the stage, health state, growth, environment, climatic conditions, and/or the purpose of cultivation of the plant.

Plants to which the composition for soil improvement provided herein can be applied (treated) are plants grown in dry environments (eg, fields, gardens, flowerpots, etc.) (eg, horticultural plants, field-grown plants, etc.), submerged Or it may be one or more selected from the group consisting of all monocotyledonous and dicotyledonous plants, or all herbaceous and woody plants consisting of freshwater cultivated crops (eg, paddy crops such as rice), hydroponic crops, and aquatic plants.

In one embodiment, the plant is food crops including rice, wheat, rye, barley, hops, corn, soybean, potato, wheat, red bean, oat, millet and sorghum; vegetable crops including Arabidopsis thaliana, Chinese cabbage, radish, red pepper, strawberry, tomato, watermelon, cucumber, cabbage, melon, pumpkin, green onion, onion and carrot; specialty crops including ginseng, tobacco, cotton, forage, grass, sesame, sugar cane, sugar beet, perilla, peanut, rapeseed, grass and castor; fruit trees, including apple trees, pear trees, jujube trees, peaches, poplars, grapes, tangerines, persimmons, plums, apricots and bananas; woody trees including pine, palm oil and eucalyptus; flowers including roses, gladiolus, gerberas, carnations, chrysanthemums, lilies and tulips; And it may be at least one selected from the group consisting of ryegrass, red clover, orchard grass, alfalfa, tall fescue, and feed crops including perennial ryegrass, but is not limited thereto.

In one embodiment, the plant is poplar (Poplar), Caragana (Caragana), corn (maize), ryegrass (ryegrass), rice, rye, wheat, barley, hops, soybeans, potatoes, red beans, oats, sorghum, millet , Arabidopsis, Chinese cabbage, radish, red pepper, strawberry, tomato, watermelon, cucumber, cabbage, melon, pumpkin, green onion, onion, carrot, ginseng, tobacco, cotton, sesame, sugar cane, sugar beet, perilla, peanut, rapeseed, Apple tree, pear tree, jujube tree, peach, poplar, grape, tangerine, persimmon, plum, apricot, banana, rose, gladiolus, gerbera, carnation, chrysanthemum, lily, tulip, red clover, orchard grass, alfalfa, tall fescue, and It may be at least one selected from the group consisting of perennial ryegrass.

In one example, the composition for improving the soil may further include at least one selected from the group consisting of a weight element, a trace element, a thickener, a stabilizer, a tackifier, and a pH adjuster.

The thickener increases the viscosity of the composition for soil improvement. Thickeners include, for example, xanthan gum, guar gum, gum arabic, gum tragacanth, galactan, carob gum, karaya gum, carrageenan, acacia gum, cannan, quince seed (quince), Algecoloid (brown algae extract), starch (derived from rice, corn, potato, wheat, etc.), glycyrrhizin, alginin, sodium alginate, collagen, alginate, gelatin ( gelatin), furcellaran, carrageenan, casein, locust bean gum, pectin, chitosan, albumin, dextran, succinoglucan, pullulan , tragacanthin, hyaluronic acid, pectin, alginic acid, agar, galactomannans, beta-cyclodextrin, amylase, polyethylene Oxide (PEO), polyethylene glycol (PEG), polyvinylpyrrolidone (PVP), polyvinyl alcohol (PVA), polyacrylic acid (PAA), N-(2-hydroxypropyl)methacrylamide (HPMA), di Vinyl ether-maleic anhydride (DIVEMA), polyphosphate, polyphosphazene, methyl cellulose, methyl ethyl cellulose, ethyl cellulose, microcrystalline cellulose, hydroxyethyl cellulose, hydroxybutyl methyl cellulose, hydroxypropyl cellulose, hydroxy Roxypropylmethylcellulose, hydroxyethylmethylcellulose, hydroxypropylmethylcellulose stearoxyether, carboxymethylhydroxyethylcellulose, alkylhydroxyethylcellulose, nonoxynylhydroxyethylcellulose, sodium cellulose sulfate, methylcellulose, methylethylcellulose , ethyl cellulose, carboxymethyl cellulose (CMC), locust bean gum, lauric acid, welean gum, and at least one selected from pullulan) It is not necessarily limited to these, and all are possible as long as it is used as a thickener in the art.

According to one example, the thickener content in the composition for soil improvement is 10 parts by weight or less, 9 parts by weight or less, 8 parts by weight or less, 7 parts by weight or less, 6 parts by weight or less, 5 parts by weight or less, based on 100 parts by weight of the composition; 4 parts by weight or less, 3 parts by weight or less, 2 parts by weight or less, 1 part by weight or less, 0.1 parts by weight or less, 0.01 to 10 parts by weight, 0.01 to 5 parts by weight, 0.01 to 1 parts by weight, or 0.01 to 1 parts by weight. have. The composition for soil improvement including a thickener in an amount within the above range is superior to a composition for soil improvement including a thickener in an amount outside the above range for promoting plant growth, promoting biomass, and/or improving soil.

The tackifier (tackifier) increases the tackiness of the composition for soil improvement. Tackifiers include rosin, hydrogenated rosin, polymerized rosin, male rosin, rosin glycerin, modified phenolic resin, rosin and its modified products such as rosin acid and rosin ester; terpene-based resins such as terpene resins, terpene-phenol resins, and terpene-styrene resins; petroleum resins such as C5 petroleum resin, C9 petroleum resin, bicyclic ronadiene petroleum resin, hydrogenated petroleum resin; Resin emulsion such as rosin emulsion, TPR water based resin, 2402 resin emulsion, petroleum resin emulsion, coumaronindene resin; phenolic resins such as cashew oil modified phenolic resin and tall oil modified phenolic resin; Polymethylstyrene resin, ketonealdehyde resin, xylene formaldehyde resin, rubber, zirconic acid, polylysine, glutaraldehyde, glyoxal, hexamethylenetetramine , butanetetracarboxylic acid (butanetetracarboxylic acid), and at least one selected from aconic acid (aconic acid), but is not necessarily limited thereto, and any one used as a tackifier in the art is possible.

According to an example, the content of the tackifier in the composition for soil improvement is 10 parts by weight or less, 9 parts by weight or less, 8 parts by weight or less, 7 parts by weight or less, 6 parts by weight or less, 5 parts by weight based on 100 parts by weight of the composition. or less, 4 parts by weight or less, 3 parts by weight or less, 2 parts by weight or less 1 part by weight or less, 0.1 parts by weight or less, 0.01 to 10 parts by weight, 0.01 to 1 parts by weight, or 0.1 to 1 parts by weight. The composition for soil improvement comprising a tackifier in an amount within the above range is superior to the composition for soil improvement including a tackifier in an amount outside the range, which promotes plant growth, promotes biomass, and/or improves soil do.

The pH adjusting agent is a composition for soil improvement in a pH range (eg, 2 to 11, 2 to 9.5, or 2 to 8.5) capable of promoting plant growth, promoting biomass, and/or enhancing the soil improvement effect. As long as it is a material that can maintain it, it can be used without limitation. The pH adjusting agent may use any known buffer without limitation, for example, tromethamine, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), HEPPS (3-[4-(2-hydroxyethyl)piperazin) -1-yl]propane-1-sulfonic acid), TAPS (3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propane-1-sulfonic acid), MES (2- (N-morpholino)ethanesulfonicacid), TES (2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]ethanesulfonic acid), BES (N,N-bis(2-hydroxyethyl)- 2-aminoethanesulfonic acid), TAPSO (3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]-2-hydroxypropane-1-sulfonic acid), POPSO (Piperazine-N,N' -bis(2-hydroxypropanesulfonic acid)), HEPPSO (N-(Hydroxyethyl)piperazine-N'-2-hydroxypropanesulfonic acid), DIPSO (3-(N,N-Bis[2-hydroxyethyl]amino)-2-hydroxypropanesulfonic acid ), glycinamide, bicine, tricine, acetamidoglycine, MOPS (3-morpholinopropane-1-sulfonic acid), mixtures thereof, and derivatives thereof It may be one or more selected from the group.

According to an example, in the composition for improving soil, the content of the pH adjuster is 10 parts by weight or less, 9 parts by weight or less, 8 parts by weight or less, 7 parts by weight or less, 6 parts by weight or less, 5 parts by weight based on 100 parts by weight of the composition. or less, 4 parts by weight or less, 3 parts by weight or less, 2 parts by weight or less, 1 part by weight, 0.1 parts by weight or less, 0.01 to 10 parts by weight, 0.01 to 1 parts by weight, or 0.1 to 1 parts by weight. The composition for soil improvement comprising a pH adjuster in an amount within the above range is superior to the composition for soil improvement comprising a pH adjuster in an amount outside the above range, in promoting plant growth, promoting biomass, and/or improving soil.

In one example, the stabilizer may improve phase stability by enhancing dispersion of an active ingredient in an aqueous solution composition, for example, in a composition for soil improvement. The stabilizer is, for example, glycerol, alkylene glycol, dialkylene glycol, benzenediol, benzene triol, dial alcohol amine, trial alcohol amine, arabitol, mannitol, isomalt, xylitol, sorbitol, maltitol, erythritol. , ribitol, dulcitol, lactitol, threitol, iditol, polyglycitol, methanol, ethanol, propan-2-ol, butan-1-ol, pentan-1ol, ethane-1,2-diol ( ethane-1,2-diol), propane-1,2-diol, propane-1,2,3-triol, butane- 1,3-diol (butane-1,3-diol), butane-1,2,3,4-tetraol (butane-1,2,3,4-tetraol), pentane-1,2,3,4 ,5-pentol (pentane-1,2,3,4,5-pentol), hexane-1,2,3,4,5,6-hexol (hexane-1,2,3,4,5,6- hexol), heptane-1,2,3,4,5,6,7-heptane-1,2,3,4,5,6,7-heptol, pro-2-en-1-ol ( Prop-2-ene-1-ol), 3,7-dimethylocta-2,6-dien-1-ol (3,7-Dimethylocta-2,6-dien-1-ol), pro-2-yn- 1-ol (Prop-2-yn-1-ol), cyclohexane-1,2,3,4,5,6-hexol (cyclohexane-1,2,3,4,5,6-hexol), 2 -(2-propyl)-5-methylcyclohexan-1-ol (2-(2-propyl)-5-methyl-cyclohexane-1-ol), C2-C10 alkylenediamine, C2-C10 alkenylenediamine, phenylenediamine, and at least one selected from the group consisting of n-amino(C1-C5)alkyl (C1-C5)alkanediamine, but is not limited thereto, and any stabilizer used in the art is possible. .

According to an example, in the composition for soil improvement, the content of the stabilizer is 10 parts by weight or less, 9 parts by weight or less, 8 parts by weight or less, 7 parts by weight or less, 6 parts by weight or less, 5 parts by weight or less based on 100 parts by weight of the composition. , 4 parts by weight or less, 3 parts by weight or less, 2 parts by weight or less, 1 part by weight, 0.1 parts by weight or less, 0.01 to 10 parts by weight, 0.01 to 1 parts by weight, or 0.1 to 1 parts by weight. The composition for soil improvement comprising a stabilizer in an amount within the above range is superior to the composition for soil improvement including a stabilizer in an amount outside the above range for promoting plant growth, promoting biomass, and/or improving soil.

The weight element may be nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), etc. as nutrients, and the trace elements are iron (Fe), manganese (Mn), copper (Cu), zinc (Zn), boron (B), molybdenum (Mo), chlorine (Cl) and/or salts thereof. In one example, the content of the trace element included in the composition for soil improvement is 0.01 to 20 wt%, 0.1 to 10 wt%, 1 to 10 wt%, 0.01 wt% or more, 0.1 wt% based on the total weight of organic solids or more, 1 wt% or more, 2.5 wt% or more, 5 wt% or more, 10 wt% or more, 5 to 20 wt%, 5 to 15 wt%, or 5 to 10 wt%. In one example, the content of the weight element included in the composition for soil improvement is 0.01 to 20 wt%, 0.1 to 10 wt%, 1 to 10 wt%, 0.01 wt% or more, 0.1 wt% based on the total weight of organic solids or more, 1 wt% or more, 2.5 wt% or more, 5 wt% or more, or 10 wt% or more.

In one example, based on 100 parts by weight of the solid content of the composition for soil improvement, the content of the trace element is 0.01 to 100 parts by weight, 0.1 to 90 parts by weight, 1 to 80 parts by weight, 2.5 to 70 parts by weight, 5 to 60 parts by weight. , 5 to 50 parts by weight, 5 to 20 parts by weight, or 5 to 10 parts by weight.

In one example, the content of the weight element based on 100 parts by weight of the solid content of the composition for soil improvement is 0.01 to 100 parts by weight, 0.1 to 90 parts by weight, 1 to 80 parts by weight, 2.5 to 70 parts by weight, 5 to 60 parts by weight. , 5 to 50 parts by weight, 5 to 20 parts by weight, or 5 to 10 parts by weight.

In one example, based on 100 parts by weight of the solid content of the composition for soil improvement, the sum of weight elements and trace elements is 0.01 to 100 parts by weight, 0.1 to 90 parts by weight, 1 to 80 parts by weight, 2.5 to 70 parts by weight, 5 to 60 parts by weight, 5 to 60 parts by weight, 10 to 60 parts by weight, 10 to 50 parts by weight, or 10 to 40 parts by weight.

By including a weight element and a trace element in the content of the above range in the composition for soil improvement according to an example, soil binding force, physical and/or chemical soil improvement, and/or wettability is increased, and plant growth can be further improved have.

In one example, the solid content in the composition for soil improvement is 0.1 to 70 parts by weight, 1 to 70 parts by weight, 1 to 60 parts by weight, 1 to 50 parts by weight, 10 to 50 parts by weight based on 100 parts by weight of the composition for soil improvement. parts, 10 to 40 parts by weight, 20 to 40 parts by weight, or 20 to 30 parts by weight. The composition for soil improvement comprising a solid content within the above range can maintain adhesion without forming a precipitation, so it can be applied to plants, plant seeds, soil, and plant planting soil (eg, sprayed on the soil or mixed with soil) It is easy to do, and when the solid content exceeds 70 parts by weight, a precipitate is formed, and the effect as a composition for soil improvement may be reduced. Even if the content of the solid content is reduced, precipitation in the composition is not formed or the moisture content is not lost, so the content of the solid content may be adjusted to a level of 0.1 to 50 parts by weight, or 0.1 to 30 parts by weight, depending on the applied field. . The solid content may refer to the solid content of lysine and citric acid (solid content consisting of lysine and citric acid).

In the composition for improving soil, citric acid and lysine may contain a condensate having citric acid and lysine as units. For example, the condensate may be a dimer, trimer or oligomer, and the content of the condensate is 20 parts by weight or less, 10 parts by weight or less, 1 part by weight or less based on 100 parts by weight of the sum of citric acid and lysine contents. and may not contain a condensate. When the condensate is included within the above range, the moisture content and fluidity of the composition for soil improvement are excellent, and the liquid state is maintained so that the composition for soil improvement is easy to spray on the soil or mix with the soil, so that it exceeds the above range (exceeds) The effect of promoting plant growth, promoting biomass, and/or improving the soil is superior to the composition for soil improvement including the condensate as the content.

In one example, the composition for soil improvement is 10:1 to 1:10, 5:1 to 1:5, 5:1 to 1:3, 5:1 to 1:2, 5:1 to lysine and citric acid 1:1.5, 5:1 to 1:1, 1:1 to 1:5, 3:1 to 1:5, 3:1 to 1:3, 3:1 to 1:2, 3:1 to 1: 1.5, 3:1 to 1:1, 1.5:1 to 1:5, 1.5 to 1:3, 1.5:1 1.5:1 to 1:2, 1.5:1 to 1:1.5, 1.5:1 to 1:1.5 Alternatively, it may be included in a molar ratio of 1.5:1 to 1:1. The composition for soil improvement comprising lysine and citric acid in a molar ratio within the above range is superior to the composition comprising lysine and citric acid in a molar ratio outside the above range for promoting plant growth, promoting biomass, and/or improving soil.

Another aspect provides a method for improving soil, comprising treating the soil with a composition for soil improvement comprising lysine and citric acid.

The above "soil improvement" (or soil improvement) is applied to various types of soil classified as areas where normal survival or growth of plants such as crops or trees is difficult, such as alkaline soil, salt-containing soil, clay soil, sandy soil, etc. promotes planting and growth, corrects soil acidity and buffers, promotes plant (crop) growth and root growth, prevents soil hardening, improves soil permeability to help proper root growth, soil It may mean preventing leaching of fertilizer components, preventing salt accumulation, and/or preventing soil erosion.

In one example, "soil improvement" may be at least one selected from the group consisting of the following (1) to (7):

(1) maintaining the moisture content of the soil;

(2) increase the moisture content of the soil;

(3) decrease the bulk density of the soil;

(4) maintaining the bulk density of the soil;

(5) prevent erosion of the soil;

(6) prevention of spillage of fertilizer components in the soil; and

(7) Maintaining soil acidity in the range of greater than pH4.5 to less than pH8.0.

In one example, "soil improvement" means that the composition for soil improvement is treated in the soil when the composition for soil improvement is not treated or when the composition for soil improvement is treated with water or a commercial soil improvement composition is treated in the following (1) to ( 7) may be one showing the effect of one selected from the group consisting of:

(1) maintaining the moisture content of the soil;

(2) increase the moisture content of the soil;

(3) decrease the bulk density of the soil;

(4) maintaining the bulk density of the soil;

(5) prevent erosion of the soil;

(6) prevention of spillage of fertilizer components in the soil; and

(7) Maintaining soil acidity in the range of greater than pH4.5 to less than pH8.0.

In one example, the composition for soil improvement is treated in the soil, and the amount of change in the moisture content of the soil is smaller than when the composition for soil improvement is not treated or when water or a commercial composition for soil improvement (existing composition for soil improvement) is treated. Alternatively, the moisture content can be increased or the moisture content can be maintained. Maintaining or increasing the moisture content of the soil by the composition for improving soil according to an example may be more effective in dry (or drought) soil.

In one example, the composition for soil improvement may be to increase the moisture content of the soil than when the composition for soil improvement is not treated or when the composition for soil improvement is treated by treating the soil, and the moisture content of the soil is It may be calculated by Equation (2).

In one example, the composition for soil improvement may have increased water resistance, moisture content of the composition itself, and/or hygroscopicity compared to water or a commercial soil improvement composition.

Commercial soil improvement compositions in dry soils such as sandy soils have the disadvantage that they are easily washed away by melted rain or run down to a depth of soil that plants are difficult to use, so that the usable amount of plants is easily reduced, but in one example The composition for improving soil according to the present invention has excellent water resistance, and thus it is easy to maintain an usable amount of plants in the soil.

The composition for soil improvement according to an example may prevent drying of the soil by increasing the moisture content and/or hygroscopicity of the composition itself, and the effect may be superior to that of a commercial soil improvement composition.

In one example, the composition for soil improvement is treated in the soil to reduce the bulk density of the soil compared to the case in which the composition for soil improvement is not treated or a commercial composition for soil improvement is treated, or optimal bulk density for crop growth can be maintained.

The bulk density (bulk density) is a value obtained by dividing the weight of the soil by the total volume (total volume) of the soil, and represents the density of the soil. If the content of organic matter and/or moisture that is less than that of the soil grains in the same volume is large, the bulk density is lowered, and the more compact the soil is, the higher the bulk density is.

The fertilizer component (fertilizer) may be a nitrogen source, a phosphoric acid source, a potassium source, a calcium source, a sulfur source, a magnesium source, or a combination thereof, for example, urea, potassium chloride, boron, zinc sulfate, sulfuric acid (goto) magnesium, phosphoric acid , potassium, manganese, boron, or a combination thereof. The nitrogen provided by the nitrogen source plays an important role in determining the growth and yield of crops as components of proteins, enzymes, amino acids, nucleic acids, chlorophyll, etc., and the phosphoric acid provided by the phosphate source is a component constituting the nucleic acid of cells. essential for division. Potassium provided by the potassium source is essential for balance with negative charge, osmotic pressure control, and/or water and nutrient transfer, and plays a major role in transferring nutrients made from leaves to fruits. Calcium provided by the calcium source is involved in the function of the cell membrane. Sulfur provided by the sulfur source is involved in the formation of chlorophyll as an essential component of protein. Magnesium provided by the magnesium source is an essential component of chloroplasts and is directly involved in photosynthesis, and when magnesium is insufficient, between leaf veins of green leaves is yellowed.

The composition for soil improvement according to an example is treated with the soil containing a fertilizer component compared to the case where the composition for soil improvement is not treated or the existing composition for soil improvement (commercial soil improvement composition) is treated to reduce fertilizer runoff. The reduced amount of fertilizer outflow is, for example, 1.5 times to 10 times, 1.5 times to 5 times, 1.5 times to 3 times, 1.5 times than the amount of fertilizer outflow reduction of soil that does not include the composition for soil improvement according to an example times to 2.5 times, or 2 times to 2.5 times less.

The composition for soil improvement according to an example is treated on the soil containing the fertilizer component compared to the case where the composition for soil improvement is not treated or the existing composition for soil improvement is treated, so that the fertilizer outflow is reduced, thereby increasing the amount of the fertilizer component in the soil It may be to keep

In the composition for soil improvement according to an embodiment, erosion of the soil can be prevented than when the composition for soil improvement is not treated or when the conventional composition for soil improvement is treated, and the erosion of the soil is caused by rainfall and/or wind it could be In one embodiment, the composition for soil improvement may be to prevent erosion of the soil with an inclination angle of 5 to 80 °, 10 to 60 °, 15 to 50 °, or 20 to 40 °, or 10 to 20 °. In one example, erosion is prevented, the composition for soil improvement according to an example is higher than the case in which the soil improvement composition is not treated or the soil is eroded by wind and/or rainfall than when the existing soil improvement composition is treated. It may mean that the (step difference) is small.

The soil treated with the composition for soil improvement comprising lysine and citric acid according to an example may maintain an acidity of greater than pH4.5 to less than pH8.0. When the soil is acidified and has an acidity of pH 4.5 or less, the hydrogen ions of the soil water increase, and aluminum ions in the soil particles that are toxic to trees are eluted, causing growth disorders of trees and binding with phosphoric acid to provide nutrients. may cause deficiency. On the other hand, when the soil is alkalized and has an acidity of pH 8.0 or higher, calcium ions and phosphoric acid combine and the solubility of other trace elements is lowered, which may cause nutritional and physiological disorders of trees due to nutrient deficiency.

In one example, the step of treating the soil with the composition for soil improvement is at least one selected from the group consisting of immersion method, soil mixing method, soil spraying method, application treatment, fumigation treatment, spraying, irrigation, and indirect absorption method. It may be carried out in this way. The method for treating the soil with the composition for soil improvement is the same as described above.

In one example, the composition for improving the soil is 100 to 4500 kg / ha, 300 to 4500 kg / ha, 300 to 2400 kg / ha, 600 to 900 kg / ha, 450 to 4500 kg / ha, 600 to 4500 kg / ha, 900 to 4500 kg / The soil may be treated at a concentration of ha, 1800 to 4500 kg/ha, 900 to 4000 kg/ha, 900 to 4000 kg/ha, 1200 to 3000 kg/ha, or 1500 to 3000 kg/ha.

In one example, the soil may further include a fertilizer component or be fertilized with fertilizer. The fertilizer components are the same as described above.

The composition for soil improvement according to an example may be treated with soil including a fertilizer component, so that the soil improvement effect may appear synergistically. In one example, even if the soil containing the fertilizer component, when the chemical, physical, and / or microbial properties of the soil are not good, the effect of the fertilizer cannot be properly obtained, but when the composition for soil improvement according to an example is treated together, the soil It is possible to synergistically increase the effect of the fertilizer together with the improvement effect. In one example, the synergistic increase in the effect of fertilizer means that the composition for soil improvement according to an example is treated on the soil containing the fertilizer component, compared to when the commercial composition for soil improvement is treated on the soil containing the same fertilizer component. It may be to increase the amount of biomass of the plant.

In one example, the soil is 30 to 80 parts by weight of the soil having a particle size of 0.02mm or more; 10 to 40 parts by weight of soil having a particle size of less than 0.02 mm to more than 0.002 mm; and 5 to 40 parts by weight of soil having a particle size of less than 0.002 mm.

In one example, the soil is 40 to 80 parts by weight of the soil having a particle size of 0.02mm or more; 15 to 30 parts by weight of soil having a particle size of less than 0.02 mm to more than 0.002 mm; and 5 to 30 parts by weight of soil having a particle size of less than 0.002 mm.

In one example, the maintenance of the moisture content of the soil is a daytime temperature of 25 to 35 ℃ or 25 to 30 ℃; night temperature 15 to 25 ℃ or 15 to 25 ℃; 10 to 300 days, 15 to 250 days, or 20 days at a relative humidity of 10 to 80%RH, 15 to 70%RH, 20 to 60%RH, 30 to 55%RH, or 35 to 50%RH 0.1 to 90%, 0.1 to 80%, 0.5 to 80%, 1 to 60%, 1 to 50%, 50% or less, 40% or less, 30% or less, 20 % or less, 20% or less, 15% or less, 10% or less, 7% or less, 5% or less, 4% or less, 3% or less, 2% or less, 1% or less 0.9% or less, 0.8% or less, 0.7% or less, It may be 0.6% or less, 0.5% or less, 0.4% or less, or 0.3% or less.

In one example, the maintenance of the moisture content of the soil is a daytime temperature of 25 to 35 ℃ or 25 to 30 ℃; night temperature 15 to 25 ℃ or 15 to 25 ℃; 10 to 300 days, 15 to 250 days, or 20 under the conditions of 10 to 80%RH, 15 to 70%RH, 20 to 60%RH, 30 to 55%RH, or 35 to 50%RH of relative humidity The moisture content of the soil treated with the composition for soil improvement according to an example after days to 200 days has elapsed is 0.01 to 20% by weight, 0.01 to 10% by weight, 0.01 to 5% by weight of the soil treated with the commercial soil improvement composition. , 0.1 to 10% by weight, or 0.1 to 5% by weight may be higher.

By treating the composition for soil improvement according to an example, the soil may be modified into soil that can contain moisture for a long time. In one example, when the soil is reformed into a soil that can contain moisture for a long time, the amount of incoming water may be greater than the amount of water lost, thereby preventing desertification or exhibiting an effect of reforming the desert soil, and desertification. Reduction of soil loss (soil loss) may be achieved by preventing or modifying desert soil.

In one example, the solid content in the composition for soil improvement may be 0.1 to 70 parts by weight based on 100 parts by weight of the composition for soil improvement. The solid content may refer to the solid content of lysine and citric acid (solid content consisting of lysine and citric acid). The solid content is the same as described above.

In one example, the composition for soil improvement may include lysine and citric acid in a molar ratio of 5:1 to 1:5. The mixed molar ratio of lysine and citric acid included in the composition for soil improvement is the same as described above.

The composition for soil improvement used in the soil improvement method is the same as described above.

Another aspect provides a composition for improving soil, comprising lysine and citric acid.

In one example, the composition for improving soil may further include a fertilizer component. As for the synergistic effect that appears as the fertilizer component is further included, it is the same as described above.

In one example. The solid content in the composition for soil improvement may be 0.1 to 70 parts by weight based on 100 parts by weight of the composition for soil improvement. The solid content may refer to the solid content of lysine and citric acid (solid content consisting of lysine and citric acid). The solid content is the same as described above.

In one example, the composition for soil improvement may include lysine and citric acid in a molar ratio of 5:1 to 1:5. The mixed molar ratio of lysine and citric acid included in the composition for soil improvement is the same as described above.

In one example, the pH of the composition for soil improvement may be 2 to 11, 2 to 9.5, or 2 to 8.5. The composition for soil improvement having the above pH range has excellent storage stability and storage stability, and even if stored for a long time, there may be no change in formulation or quality. The composition for soil improvement has excellent effects of promoting plant growth, enhancing biomass, and/or improving soil even when used immediately after being prepared as well as used after long-term storage, and no sedimentation is formed.

The composition for soil improvement may be one in which no sediment is formed after storage or distribution for 14 days or more. For example, 14 days or more, for example, 12 months or more, for example, 24 months or more, even if stored, the composition is stable and the physical properties can be maintained as it is. In addition, the temperature of the environment in which the composition for soil improvement is stored may be -18°C to 80°C, specifically -18°C to 45°C, 0°C to 60°C, or 20°C to 40°C. Even if stored outside the temperature range, if the temperature of the environment in which the composition for soil improvement is used is within the temperature range, the formulation and quality may not be affected. For example, when stored at a low temperature, it can be used after leaving it at room temperature for a certain period of time before use.

The composition for improving the soil is the same as described above.

Another aspect provides a fertilizer composition comprising the composition for soil improvement.

In one embodiment, the fertilizer composition may further include additional components necessary for plant growth, and may be used without limitation as long as it is commonly known.

As used herein, 'fertilizer' refers to any material that supplies one or more elements necessary for normal growth of plants, and includes organic fertilizers (consisting of decomposed plant/animal substances) and inorganic fertilizers (chemical substances and inorganic substances). material), and so on.

The fertilizer composition may be formulated in various forms such as liquid, powder, pellets or granules of an appropriate size using a general formulating machine, but is not limited thereto. The fertilizer composition of the present invention formulated above may be used as it is, or air dried at room temperature, or dried by freeze drying or high temperature drying method. In order to properly use the liquid fertilizer composition in farms, it should not precipitate as a pH range usable for crops. This can be selected by considering the pH and solubility of the raw material used.

By using the composition for soil improvement according to an example, it is possible to promote plant growth or enhance biomass with excellent efficiency, improve soil, and/or inhibit soil erosion through soil surface fixation.

1 shows a ¹ H nuclear magnetic resonance spectrum (NMR) analysis result according to Reference Example 7.
2 shows the monthly rainfall and temperature in the field test area.
3 shows the soil erosion inhibitory effect of the soil preparation composition according to one embodiment. The part marked with a rectangle in FIG. 3 is the area where the soil preparation composition d-1 (left photo) and d-2 (right photo) has been sprayed, and the other parts mean the non-sprayed area where the soil preparation composition is not applied. and the yellow arrow means the height (step difference) at which the soil is eroded by wind or rainfall in the sprayed and non-sprayed areas.

The present invention will be described in more detail with reference to the following examples, but the scope of the rights is not limited to the following examples. As described above, the “soil preparation composition” or “soil preparation agent” of the following Examples was used in the same sense as the “soil improvement composition”.

Example 1. Preparation of a soil preparation composition comprising a salt of lysine and citric acid

Example 1-1. Preparation of a soil preparation composition comprising a salt of lysine and citric acid and a thickener

53.33 g of DIW (distilled water) was added to 100 g of 54 wt% L-lysine free form aqueous solution, and the mixture was diluted with stirring at room temperature (25° C.) for 30 minutes. To the diluted lysine, 47.31 g of citric acid (CA) and 1.98 g of xanthan gum were slowly added at room temperature (25° C.) and stirred for 1 hour, followed by stirring at 60° C. for 1 hour. Then, after the reaction mixture reached room temperature (25° C.), the reaction was terminated to obtain 202.62 g of soil preparation composition 1A. The solid content of lysine and citric acid in Composition 1A is about 50 parts by weight (= 50% by weight) based on 100 parts by weight of the soil preparation composition, and the mixing molar ratio of lysine and citric acid in the soil preparation composition is 1.5: It was 1. The xanthan gum content was 0.977 wt% based on the total weight of the composition, and the solvent was deionized water.

Except for changing the molar ratio of lysine and citric acid, soil preparation compositions 1B to 1G were prepared in the same manner as in the preparation method of soil preparation composition 1A.

The mixing molar ratio of lysine and citric acid included in the soil preparation compositions 1A to 1G, the solid content of lysine and citric acid, and the xantam gum content are shown in Table 1 below.

Soil preparation composition
(composition for soil improvement) Molar ratio (Lys:CA) Solid content (wt%) Xantam Gum Content (wt%) Composition 1A 1.5:1 50 0.977 Composition 1B 3:1 Composition 1C 1:3 Composition 1D 5:1 Composition 1E 1:5 Composition 1F 10:1 Composition 1G 1:10

Example 1-2. Preparation of a soil preparation composition comprising a salt of lysine and citric acid

55.31 g of DIW (distilled water) was added to 100 g of 54 wt% L-lysine free form aqueous solution, and the mixture was diluted with stirring at room temperature (25° C.) for 30 minutes. To the diluted lysine, 47.31 g of citric acid (CA) was slowly added at room temperature (25° C.) and stirred for 1 hour, followed by stirring at 60° C. for 1 hour. Then, after the reaction mixture reached room temperature (25° C.), the reaction was terminated to obtain 202.62 g of soil preparation composition 2A. In Composition 2A, the content of solids composed of lysine and citric acid was about 50 parts by weight based on 100 parts by weight of the composition, the molar ratio of lysine and citric acid was 1.5:1, and the solvent was deionized water.

Except for changing the molar ratio of lysine and citric acid, soil preparation compositions 2B to 2G were prepared in the same manner as in the preparation method of the soil preparation composition 2A.

The mixture molar ratio of lysine and citric acid included in the soil preparation compositions 2A to 2G and the solid content consisting of lysine and citric acid are shown in Table 2 below.

Soil preparation composition
(composition for soil improvement) Molar ratio (Lys:CA) Solid content (wt%) Composition 2A 1.5:1 50 Composition 2B 3:1 Composition 2C 1:3 Composition 2D 5:1 Composition 2E 1:5 Composition 2F 10:1 Composition 2G 1:10

Examples 1-3. Preparation of a soil preparation composition comprising a salt of lysine and citric acid, a thickener, and trace elements

54% by weight of L-Lysine free form (Lysine free form) was added 37.10g of DIW (distilled water) to 100g aqueous solution and diluted at the same time with stirring at room temperature (25 ℃) for 30 minutes. To the diluted lysine, 47.31 g of citric acid (CA) and 1.98 g of xanthan gum were slowly added at room temperature (25° C.) and stirred for 1 hour, followed by 0.01 g of sodium molybdate (Na ₂ MoO _{4 ·} 2H ₂ O) and boric acid (H ₃ BO ₃ ) 0.34 g, manganese sulfate (MnSO _{4 ·} H ₂ O) 4.49 g, iron sulfate (FeSO _{4 ·} 7H ₂ O) 4.54 g, copper sulfate (CuSO _{4 ·} 5H ₂ O) 2.39 g, zinc sulfate (ZnSO _{4 ·} 7H ₂ O) 4.46 g was added and stirred at 60° C. for 5 hours. Then, after the reaction mixture reached room temperature (25° C.), the reaction was terminated to obtain 202.62 g of soil preparation composition 3A. In Composition 3A, the solid content of lysine and citric acid was about 50 parts by weight based on 100 parts by weight of the composition, and the mixing molar ratio of lysine and citric acid was 1.5:1. The xanthan gum content was 0.977 wt% based on the total weight of the composition, and the solvent was deionized water.

A soil preparation composition 3B was prepared in the same manner as in the preparation method of the soil preparation composition 3A, except that xanthan gum was not added and 39.08 g of DIW was added to 100 g of a 54 wt% L-lysine preform aqueous solution.

The mixing molar ratio of lysine and citric acid included in the soil preparation compositions 3A and 3B, the solid content consisting of lysine and citric acid, and the trace element content are shown in Table 3 below.

Soil preparation composition Molar ratio (Lys:CA) solid content
(wt%) Xanthan Gum Content
(wt%) Trace element content
(wt%) Composition 3A 1.5:1 50 0.977 8.0% Composition 3B 1.5:1 50 - 8.0%

Example 2. Water resistance evaluation

Example 2-1. Preparation of soil preparation composition

By adding water to the soil preparation composition 1A (lysine and citric acid 1.5:1 molar ratio) obtained in Example 1-1, the solid content in the composition was 1% by weight (composition a-1), 2.5% by weight (composition a-2) , 5% by weight (composition a-3), 7.5% by weight (composition a-4), and 10% by weight (composition a-5) were prepared.

By adding water to the soil preparation composition 1B (lysine and citric acid 3:1 molar ratio) obtained in Example 1-1, the solid content in the composition was 1.5 wt% (composition a-6), 2 wt% (composition a-7) , and 2.5% by weight (composition a-8) was prepared.

By adding water to the soil preparation composition 1C (lysine and citric acid 1:3 molar ratio) obtained in Example 1-1, the solid content in the composition was 1.5 wt% (composition a-9), 2 wt% (composition a-10) , and 2.5% by weight (composition a-11) was prepared.

The soil preparation composition 1D (lysine and citric acid 5:1 molar ratio) obtained in Example 1-1 had a solid content of 1.5% by weight (composition a-12), 2% by weight (composition a-13), and 2.5% by weight (composition a-13) a-14), each diluted with water was used.

The soil preparation composition 1E (lysine and citric acid 1:5 molar ratio) obtained in Example 1-1 had a solid content of 1.5% by weight (composition a-15), 2% by weight (composition a-16), and 2.5% by weight (composition a-16) a-17), each diluted with water was used.

The soil preparation composition 1F (lysine and citric acid 10:1 molar ratio) obtained in Example 1-1 was diluted with water to have a solid content of 2.5% by weight (composition a-18) and used.

The soil preparation composition 1G (lysine and citric acid 1:10 molar ratio) obtained in Example 1-1 was diluted with water to have a solid content of 2.5 wt% (composition a-19) and used.

As Control A, a commercially available soil preparation containing magnesium chloride (MgCl ₂ ) as a main component was used (DUS-CON, Presto), and the solid content of the soil preparation was 20 according to the guidelines described in the soil preparation. It was used after dilution to wt% (wt%).

As control B, deionized water was used as it was.

The mixed molar ratio, solid content, and xanthan gum content of lysine and citric acid contained in compositions a-1 to compositions a-19 prepared by adding water to soil preparation compositions 1A to 1G as described above are described in Table 4 below. . In Table 4 below, the solid content means the solid content consisting of lysine and citric acid in the case of the soil preparation compositions a-1 to a-19, and the solid content in the composition in the case of the control A.

soil preparation Molar ratio (Lys:CA) Solid content (wt%) Xanthan Gum Content (wt%) a-1 1.5:1 One 0.0195 a-2 2.5 0.0489 a-3 5 0.0977 a-4 7.5 0.1466 a-5 10 0.1954 a-6 3:1 1.5 0.0293 a-7 2 0.0391 a-8 2.5 0.0489 a-9 1:3 1.5 0.0293 a-10 2 0.0391 a-11 2.5 0.0489 a-12 5:1 1.5 0.0293 a-13 2 0.0391 a-14 2.5 0.0489 a-15 1:5 1.5 0.0293 a-16 2 0.0391 a-17 2.5 0.0489 a-18 10:1 2.5 0.0489 a-19 1:10 2.5 0.0489 Control A
(Commercial Cooking Agent) - 20 - Control B (deionized water) - - -

Example 2-2. Water resistance evaluation

The following method for water resistance to the soil preparation composition (composition a-1 to a-3, and a-5) prepared in Example 2-1, control A (commercial preparation), and control B (deionized water) was evaluated according to

After the soil preparation composition (or control) was applied to a glass column filled with sand at a constant height, water resistance was evaluated according to the penetration height (depth) of the soil preparation composition (or control). A composition having excellent water resistance has a small penetration height (depth) (thin), and a composition with poor water resistance, that is, a composition soluble in water, has a large penetration height (depth) (deep).

A transparent glass column (diameter 3.6 cm, height 18 cm) was filled with 250 g of sand to a height of 16 cm. 12.5 g of the soil preparation composition (a-1 to a-3, and a-5), control A, or control B prepared in Example 1 was applied to the top of the sand column filled in each glass column, 180 The penetration rate was evaluated by Equation 1 below by measuring the height (depth) at which the composition penetrated into the sand by gravity after a lapse of minutes. The evaluation results are shown in Table 5 below. The evaluation temperature was 25°C±1°C.

[Equation 1]

Penetration (%) = [H ₁ / H ₀ ×100]

H ₀ : Total height of sand filled in glass column

H ₁ : The height of the sand penetrated by the soil preparation composition

composition Penetration [%] a-1 100 a-2 81 a-3 50 a-5 44 Control A 100 Control B 100

As shown in Table 5, the soil preparation compositions (a-1 to a-3 and a-5) prepared in Example 2-1 showed an equal or less permeation rate compared to the controls A and B, as a result It showed equal or higher water resistance.

In sandy soils, soil preparations such as inorganic salts are easily washed away by rainfall or easily run down to the soil depth that plants cannot use, making it difficult to maintain the amount of usable soil preparations in the soil In the case of , there was a disadvantage that the effect of catching and maintaining moisture easily disappears. The soil preparation compositions a-1 to a-3 and a-5 prepared in Example 2-1 showed equal or higher water resistance even though the solid content was 2 to 20 times less than that of Control A, which is a commercial soil preparation composition. It was. Therefore, it can be seen that the soil preparation composition according to an example can easily maintain an usable amount of plants in the soil, and thus can maintain the moisture content in the soil.

Example 3. Analysis of moisture content and hygroscopicity of the composition according to the mixing molar ratio of lysine and citric acid

Example 3-1. Preparation of soil preparation composition

By adding water to the soil preparation composition 2A (lysine and citric acid = 1.5:1 molar ratio) obtained in Example 1-2, the solid content consisting of lysine and citric acid in the composition was 5 wt% (composition b-1), 10 wt% (composition b-2), 20 wt% (composition b-3), 25 wt% (composition b-4), 30 wt% (composition b-5), 40 wt% (composition b-6) and 50 wt% A composition was prepared in % by weight (composition b-7).

By adding water to the soil preparation composition 2B (lysine and citric acid = 3:1 molar ratio) obtained in Example 1-2, the solid content consisting of lysine and citric acid in the composition was 5% by weight (composition b-8), 10% by weight % (composition b-9), 20% by weight (composition b-10), and 25% by weight (composition b-11) were prepared.

By adding water to the soil preparation composition 2C (lysine and citric acid = 1:3 molar ratio) obtained in Example 1-2, the solid content consisting of lysine and citric acid in the composition was 5 wt% (composition b-12), 10 wt. % (composition b-13), 20% by weight (composition b-14), and 25% by weight (composition b-15).

By adding water to the soil preparation composition 2D (lysine and citric acid = 5:1 molar ratio) obtained in Example 1-2, the solid content consisting of lysine and citric acid in the composition was 5% by weight (composition b-16), 10% by weight % (composition b-17), 20 wt% (composition b-18), and 25 wt% (composition b-19) were prepared.

By adding water to the soil preparation composition 2E (lysine and citric acid = 1:5 molar ratio) obtained in Example 1-2, the solid content consisting of lysine and citric acid in the composition was 5 wt% (composition b-20), 10 wt. % (composition b-21), 20% by weight (composition b-22), and 25% by weight (composition b-23).

By adding water to the soil preparation composition 2F (lysine and citric acid = 10:1 molar ratio) obtained in Example 1-2, the solid content consisting of lysine and citric acid in the composition was 10% by weight (composition b-24), 20% by weight % (composition b-25), and 25% by weight (composition b-26).

By adding water to the soil preparation composition 2G (lysine and citric acid = 1:10 molar ratio composition) obtained in Example 1-2, the solid content consisting of lysine and citric acid in the composition was 10% by weight (composition b-27), 20 Compositions were prepared which were % by weight (composition b-28), and 25% by weight (composition b-29).

The mixed molar ratio of lysine and citric acid in the b-1 to b-29 compositions prepared by adding water to the soil preparation compositions 2A to 2G as described above, and the solid content consisting of lysine and citric acid are shown in Table 6 below.

composition Molar ratio (Lys:CA) Solid content (% by weight) b-1 1.5:1 5 b-2 10 b-3 20 b-4 25 b-5 30 b-6 40 b-7 50 b-8 3:1 5 b-9 10 b-10 20 b-11 25 b-12 1:3 5 b-13 10 b-14 20 b-15 25 b-16 5:1 5 b-17 10 b-18 20 b-19 25 b-20 1:5 5 b-21 10 b-22 20 b-23 25 b-24 10:1 10 b-25 20 b-26 25 b-27 1:10 10 b-28 20 b-29 25

By adding water to the soil preparation composition 1A (lysine and citric acid = 1.5:1 molar ratio) obtained in Example 1-1, the solid content consisting of lysine and citric acid in the composition was 5 wt% (composition c-1), 10 wt. % (composition c-2), 20% by weight (composition c-3), 25% by weight (composition c-4), 30% by weight (composition c-5), 40% by weight (composition c-6), and 50 A composition was prepared in % by weight (composition c-7).

By adding water to the soil preparation composition 1B (lysine and citric acid = 3:1 molar ratio) obtained in Example 1-1, the solid content consisting of lysine and citric acid in the composition was 5 wt% (composition c-8), 10 wt. % (composition c-9), 20% by weight (composition c-10), and 25% by weight (composition c-11) were prepared.

By adding water to the soil preparation composition 1C (lysine and citric acid = 1:3 molar ratio) obtained in Example 1-1, the solid content consisting of lysine and citric acid in the composition was 5 wt% (composition c-12), 10 wt. % (composition c-13), 20% by weight (composition c-14), and 25% by weight (composition c-15) were prepared.

By adding water to the soil preparation composition 1D (lysine and citric acid = 5:1 molar ratio) obtained in Example 1-1, the solid content consisting of lysine and citric acid in the composition was 5 wt% (composition c-16), 10 wt. % (composition c-17), 20 wt% (composition c-18), and 25 wt% (composition c-19) were prepared.

By adding water to the soil preparation composition 1E (lysine and citric acid = 1:5 molar ratio) obtained in Example 1-1, the solid content consisting of lysine and citric acid in the composition 5 wt% (composition c-20), 10 wt% (Composition c-21), 20% by weight (composition c-22), and 25% by weight (composition c-23) were prepared.

By adding water to the soil preparation composition 1F (lysine and citric acid = 10:1 molar ratio) obtained in Example 1-1, the solid content consisting of lysine and citric acid in the composition was 25% by weight (composition c-24) and 50% by weight % (composition c-25) was prepared.

By adding water to the soil preparation composition 1G (lysine and citric acid = 1:10 molar ratio) obtained in Example 1-1, the solid content consisting of lysine and citric acid in the composition was 25% by weight (composition c-26), and 50 A composition was prepared in % by weight (composition c-27).

As the control group D, a commercially available soil preparation (DUS-CON, Presto) containing magnesium chloride (MgCl ₂ ) as a main component was prepared. According to the guidelines described in the soil preparation, the solid content was diluted to 10% by weight (composition D-1), 20% by weight (composition D-2), and 25% by weight (composition D-3), respectively.

As control E, deionized water was used as it was.

The mixed molar ratio of lysine and citric acid, the solid content consisting of lysine and citric acid, and the xanthan gum content in the c-1 to c-27 compositions prepared by adding water to the soil preparation compositions 1A to 1G as described above are shown in Table 7 below. described.

Molar ratio (Lys:CA) solid content
(weight%) Xanthan Gum Content (wt%) c-1 1.5:1 5 0.0977 c-2 10 0.1954 c-3 20 0.3908 c-4 25 0.4885 c-5 30 0.5862 c-6 40 0.7816 c-7 50 0.977 c-8 3:1 5 0.0977 c-9 10 0.1954 c-10 20 0.3908 c-11 25 0.4885 c-12 1:3 5 0.0977 c-13 10 0.1954 c-14 20 0.3908 c-15 25 0.4885 c-16 5:1 5 0.0977 c-17 10 0.1954 c-18 20 0.3908 c-19 25 0.4885 c-20 1:5 5 0.0977 c-21 10 0.1954 c-22 20 0.3908 c-23 25 0.4885 c-24 10:1 25 0.4885 c-25 50 0.977 c-26 1:10 25 0.4885 c-27 50 0.977 Control D-1 - 10 - Control D-2 - 20 - Control D-3 - 25 - Control E
(deionized water) - - -

For each soil preparation composition, soil moisture content, composition moisture content, and hygroscopicity were evaluated according to the following method.

Example 3-2. Evaluation of soil moisture content according to soil preparation treatment (efficiency of water holding capacity in soil)

Place the mixed soil at a constant thickness of 2.5 cm in a 7.8 cm horizontal and 7.8 cm plastic container, and mix the soil preparation composition prepared in Example 3-1, Control D, or Control E, respectively, by spray spraying. sprayed on the surface. The spray amount of each composition is shown in Tables 8 to 12 below. For mixed soil, 87.6w/w% of sand with an average particle size of 142.3㎛ (d10 68.31㎛, d50 128.1㎛, d90 229.8㎛) and 12.4w/w% of loess with a particle size of 400mesh or less were mixed, and the moisture content was set to 18.6w/w%. adjusted and used.

Then, by measuring the change in the weight of the mixed soil for 24 hours while maintaining a temperature of 25 ° C ± 1 ° C and a relative humidity (RH) of 20% ± 5%, the moisture reduction rate of the soil is calculated according to Equation 3 below, and mathematical According to Equation 2, the relative moisture content of the control group E (water treatment group) was evaluated. Some of the evaluation results are shown in Tables 8 to 12. In Equation 2, the moisture reduction rate of the soil after water spray was calculated using Equation 3 below using water instead of the soil preparation composition.

The lower the moisture reduction rate, the lower the loss of moisture compared to the mixed soil containing pure water (control group E), and the higher the relative moisture content, the higher the moisture retention rate compared to the mixed soil treated with pure water (control group E). do.

[Equation 2]

Relative moisture content (%) = [1 - (reduction rate of soil moisture after spraying composition / ratio of moisture loss in soil after spraying with water)]×100

[Equation 3]

Moisture reduction rate of soil after spraying composition (or water) = [(weight of mixed soil immediately after spraying composition (or water) - weight of mixed soil after 24 hours)/weight of mixed soil immediately after spraying composition (or water)] ×100

composition Molar ratio (Lys:CA) Xanthan Gum Content (wt%) Solid content (wt%) surface spray amount
(g/m ² ) relative wettability
(%) b-1 1.5:1 - 5 40 34.4 c-1 0.0977 33.9 b-2 - 10 42.2 c-2 0.1954 42.1 b-8 3:1 - 5 89.4 b-9 - 10 90.9 b-12 1:3 - 5 88.5 b-13 - 10 96.2 b-16 5:1 - 5 103.8 b-17 - 10 104.7 b-20 1:5 - 5 66.3 b-21 - 10 91.2 Control D
(D-1) - - 10 -8.6 Control E - - 0 0

composition Molar ratio (Lys:CA) Xanthan Gum Content (wt%) Solid content (wt%) surface spray amount
(g/m ² ) relative wettability
(%) b-1 1.5:1 - 5 80 44.5 c-1 0.0977 42.1 b-2 - 10 46.7 c-2 0.1954 50.5 b-8 3:1 - 5 126.3 b-9 - 10 95.4 b-12 1:3 - 5 88.3 b-13 - 10 100.9 b-16 5:1 - 5 97.9 b-17 - 10 104.2 b-20 1:5 - 5 81.5 b-21 - 10 82.5 Control D
(D-1) - - 10 6.8 Control E - - 0 0

composition Molar ratio (Lys:CA) Xanthan Gum Content (wt%) Solid content (wt%) surface spray amount
(g/m ² ) relative wettability
(%) b-1 1.5:1 - 5 160 52.0 c-1 0.0977 55.3 b-2 - 10 52.3 c-2 0.1954 62.2 b-8 3:1 - 5 93.4 b-9 - 10 99.2 b-12 1:3 - 5 89.2 b-13 - 10 98.6 b-16 5:1 - 5 109.0 b-17 - 10 95.4 b-20 1:5 - 5 63.3 b-21 - 10 82.5 Control D
(D-1) - - 10 -5.2 Control E - - 0 0

composition Molar ratio (Lys:CA) Xanthan Gum Content (wt%) Solid content (wt%) surface spray amount
(g/m ² ) relative wettability
(%) b-1 1.5:1 - 5 320 55.1 c-1 0.0977 57.0 b-2 - 10 55.8 c-2 0.1954 63.9 b-8 3:1 - 5 86.1 b-9 - 10 87.2 b-12 1:3 - 5 80.3 b-13 - 10 87.3 b-16 5:1 - 5 87.0 b-17 - 10 84.6 b-20 1:5 - 5 64.6 b-21 - 10 49.0 Control D
(D-1) - - 10 -6.8 Control E - - 0 0

composition Molar ratio (Lys:CA) Xanthan Gum Content (wt%) Solid content (wt%) surface spray amount
(g/m ² ) relative wettability
(%) b-1 1.5:1 - 5 640 62.9 c-1 0.0977 66.1 b-2 - 10 64.3 c-2 0.1954 80.3 b-8 3:1 - 5 75.9 b-9 - 10 84.0 b-12 1:3 - 5 64.8 b-13 - 10 72.6 b-16 5:1 - 5 82.9 b-17 - 10 68.5 b-20 1:5 - 5 64.2 b-21 - 10 70.2 b-24 10:1 - 10 0.4 b-27 1:10 - 10 2.1 Control D
(D-1) - - 10 -5.9 Control E - - 0 0

In Tables 8 to 12, when the relative moisture content is the same as that of water, the relative moisture content is 0%, and the moisture reduction rate of the soil sprayed with the soil preparation composition is 0 (no decrease in moisture after 24 hours) The moisture content is 100%, and when the moisture reduction rate of the soil sprayed with the soil preparation composition is negative (in the case of an increase in soil weight rather than after 24 hours, the effect is very good), the relative moisture content exceeds 100%, and the soil preparation composition is sprayed When the moisture loss rate of the soil is greater than that of water spray (more moisture than water, less effective than water), the relative moisture content is negative. As shown in Tables 8 to 12, some control groups (D-1), whose relative moisture content was calculated to be a negative value (<0), had poorer moisture content than water.

As shown in Tables 8 to 12, the soil preparation composition prepared in Example 3-1 had a significantly lower rate of moisture loss in the soil than that of Control D and Control E. That is, the soil preparation composition was treated on the soil to increase the moisture content of the soil.

Even the inorganic salt soil preparation of Control D, as described in the prior art section, is easily removed from the surface layer by dissolving by rain, flowing into river water, etc. , corrodes roads, steel structures, etc. existing on the soil surface, thereby reducing their durability, and the use of an excessive amount of inorganic salt soil preparation has limitations in causing environmental problems such as soil acidification and plant death.

Example 3-3. Evaluation of composition wetness (inherent efficiency of water holding capacity)

25 g each of the soil preparation composition prepared in Example 3-1 and the control E were put in a plastic container with a width of 7.8 cm and a length of 7.8 cm.

Then, by measuring the change in weight of the composition for 24 hours while maintaining a temperature of 40 ° C ± 1 ° C and a relative humidity (RH) of 10% ± 5%, the moisture reduction rate of the composition is calculated according to Equation 4 below Wetness (%) was evaluated. Some of the evaluation results are shown in Table 13. It means that the lower the moisture content (%) of the composition, the higher the amount of moisture loss contained in the composition.

[Equation 4]

Moisture content (%) of the composition = [(weight of the added composition - the weight of the composition lost after 24 hours) / weight of the added composition] × 100

composition Molar ratio (Lys:CA) Xanthan Gum Content (wt%) Solid content (wt%) Moisture content (%) after 24h b-4 1.5:1 - 25 32.5 c-4 0.4885 25 35.7 b-11 3:1 - 25 33.2 c-11 0.4885 25 39.3 b-15 1:3 - 25 31.2 c-15 0.4885 25 37.5 b-19 5:1 - 25 31.2 c-19 0.4885 25 40.2 b-23 1:5 - 25 29.8 c-23 0.4885 25 35.3 b-26 10:1 - 25 14.6 c-24 0.4885 25 18.7 b-29 1:10 - 25 16.0 c-26 0.4885 25 28.3 Control E - - 0 0

Referring to Table 13, it can be seen that the soil preparation composition prepared in Example 3-1 has a lower moisture reduction rate of the composition compared to Control E, and thus the composition itself has excellent moisture content.

Example 3-4. Hygroscopicity

A mixture of 20 g of the soil preparation composition prepared in Example 3-1, Control D, or Control E and 20 g of sand and 20 g of sand prepared in Example 3-1 was put in a 7.8 cm horizontal and 7.8 cm plastic container, and the temperature was 60°C±1°C. dried for 3 hours.

Then, while maintaining a temperature of 25 ° C ± 1 ° C and a relative humidity (RH) of 90% ± 5%, the weight change of the soil preparation composition was measured for 22 hours, and the water absorption rate was calculated according to Equation 5 below to calculate the hygroscopicity was evaluated. In Equation 5, the weight of the soil preparation composition means the weight of the composition excluding sand. The higher the water absorption rate, the better the hygroscopicity of the soil preparation composition, and some of the evaluation results are shown in Table 14.

[Equation 5]

Moisture absorption rate (%) = [(weight of soil preparation composition after 24 hours - weight of added soil preparation composition) / weight of input soil preparation composition]×100

composition Molar ratio (Lys:CA) Xanthan Gum Content (wt%) Solid content (wt%) Moisture absorption rate (%) b-2 1.5:1 - 10 6.6 b-9 3:1 - 10.0 b-13 1:3 - 7.3 b-17 5:1 - 10.9 b-21 1:5 - 8.0 b-24 10:1 - 12.0 b-27 1:10 - 8.3 Control E - - 0 0.0 b-4 1.5:1 - 25 5.9 c-4 0.4885 7.9 b-11 3:1 - 15.5 b-15 1:3 - 6.4 b-19 5:1 - 16.2 b-23 1:5 - 9.4 b-26 10:1 - 17.4 b-29 1:10 - 12.4 Control E - - 0 0.0

Referring to Table 14, the soil preparation composition prepared in Example 3-1 has improved hygroscopicity as compared to control E (deionized water), and thus has improved moisture absorption, thereby preventing drying of the soil.

Example 4. Analysis of the effect of the soil preparation composition by the immersion method

Example 4-1. Preparation of soil preparation composition

By adding water to the soil preparation composition 1A (lysine and citric acid = 1.5:1 molar ratio) obtained in Example 1-1, the solid content of lysine and citric acid in the composition was 5 wt% (composition d-1), 10 A composition was prepared in which weight% (composition d-2), 20% by weight (composition d-3), 25% by weight (composition d-4), 30% by weight (composition d-5).

By adding water to the soil preparation composition 1B (lysine and citric acid = 3:1 molar ratio) obtained in Example 1-1, the solid content consisting of lysine and citric acid in the composition was 20% by weight (composition d-6), and 30 A composition was prepared in % by weight (composition d-7).

By adding water to the soil preparation composition 1C (lysine and citric acid = 1:3 molar ratio) obtained in Example 1-1, the solid content consisting of lysine and citric acid in the composition was 20 wt% (composition d-8), and 30 A composition was prepared in weight percent (composition d-9).

By adding water to the soil preparation composition 1D (lysine and citric acid = 5:1 molar ratio composition) obtained in Example 1-1, the solid content consisting of lysine and citric acid in the composition was 20% by weight (composition d-10), and A composition of 30% by weight (composition d-11) was prepared.

By adding water to the soil preparation composition 1E (lysine and citric acid = 1:5 molar ratio composition) obtained in Example 1-1, the solid content consisting of lysine and citric acid in the composition was 20% by weight (composition d-12), and A composition of 30% by weight (composition d-13) was prepared.

By adding water to the soil preparation composition 1F (lysine and citric acid = 10:1 molar ratio composition) obtained in Example 1-1, the solid content consisting of lysine and citric acid in the composition was 20% by weight (composition d-14) and 30 A composition was prepared in % by weight (composition d-15).

By adding water to the soil preparation composition 1G (lysine and citric acid = 1:10 molar ratio composition) obtained in Example 1-1, the solid content of lysine and citric acid in the composition was 20% by weight (composition d-16), and A composition of 30% by weight (composition d-17) was prepared.

As the control group D, commercially available polyacrylic acid (ASAP; sodium polyacrylate, Shandong Wanhua Chemical Technology Co., Ltd.) was used. ASAP is a super absorbent polymer that can absorb moisture up to 300 to 800 times its own weight. With a solid content exceeding 1% by weight, when it comes into contact with plant roots by immersion, it is biotoxic and has a negative effect on the death of plants. According to the product guidelines, ASAP was used by diluting the solid content in the composition to 1% by weight (composition D-1).

As control E, deionized water was used as it was.

The mixed molar ratio of lysine and citric acid, the solid content consisting of lysine and citric acid, and the xanthan gum content in the d-1 to d-17 compositions prepared by adding water to the soil preparation compositions 1A to 1G as described above are shown in Table 15 below. described.

composition Molar ratio (Lys:CA) Solid content (wt%) Xanthan Gum Content (wt%) d-1 1.5:1 5 0.0977 d-2 10 0.1954 d-3 20 0.3908 d-4 25 0.4885 d-5 30 0.5862 d-6 3:1 20 0.3908 d-7 30 0.5862 d-8 1:3 20 0.3908 d-9 30 0.5862 d-10 5:1 20 0.3908 d-11 30 0.5862 d-12 1:5 20 0.3908 d-13 30 0.5862 d-14 10:1 20 0.3908 d-15 30 0.5862 d-16 1:10 20 0.3908 d-17 30 0.5862 Control D-1 - One - Control E - - -

Soil moisture content, plant viability, chlorophyll concentration, and plant growth analysis were evaluated using each soil preparation composition according to the following method.

Example 4-2. Soil moisture content by immersion method (Soil water content) evaluation

The dipping root method is generally used to evaluate the efficacy of water retention as a soil preparation near the roots of trees during reforestation. How to plant a tree in the soil.

Poplar (Poplar, 0.5 to 1 year old, 1 to 1.5 m height (height) in d-1 to d-17, control D-1, or control E of about 30L of the soil preparation composition prepared in Example 4-1 ) and the root part of gold damsel (Caragana, 1 to 2 years old, 0.5 m height (height)) were immersed for 30 seconds, respectively, and then the roots were taken out from each composition and planted in a pot with a diameter of 20 cm and a depth of 30 cm. Mixed soil for each pot, 78.3 wt% of soil having an average particle size of more than 0.02 mm; 19 kg of dry soil composed of 14.9% by weight of soil with an average particle size of 0.002 mm or more - 0.02 mm or less and 6.8% by weight of soil with an average particle size of less than 0.002 mm was used. The moisture content of each soil is shown as an average value of the results measured using 30 pots per composition at the same concentration.

The initial moisture content of the dry soil was 14% by weight, and the efficacy was evaluated by dividing 30 pots per composition into the following three drought stress conditions (water content of the soil 11%, 6%, and 3% by weight). : (1) soil moisture content of 11% by weight (A, no drought stress), (2) soil moisture content of 6% by weight (B, moderate drought stress), (3) soil moisture content of 3% by weight (C, serious drought stress). Daytime temperature 25~30℃, night temperature 15~20℃, relative humidity 35~50%RH, sunshine time 269 hours days: 15 days), and the culture conditions were changed according to the drought stress conditions (for example, in the case of condition A, the amount of insolation was low and the low temperature and humid time period (daytime temperature of about 25 ℃, night temperature of about Plant culture at 15°C, relative humidity 50%RH, short sunshine period), and in case of C condition, there is a lot of insolation and high temperature and dry time (daytime temperature about 30℃, night temperature about 20℃, relative humidity 35% so that there is a lot of evaporation) RH, long periods of sunlight)).

When the soil moisture content reached the respective drought stress conditions (11 wt, 6 wt, or 3 wt%) (22 days after planting), the conditions were maintained for 2 days, and on the 24th day after planting, the soil moisture content was 14 wt. The pots were rewatered until the % was reached. Again, when the soil moisture content reaches the respective drought stress conditions (day 30), the conditions are maintained for 2 days, and on the 32nd day after planting, the pots are watered until the soil moisture content reaches 14% by weight. -rewatering). The moisture content in the soil was measured by measuring the weight change of the soil and moisture in the pot during 2 cycles of rewatering and drought-rewatering, and the results are shown in Tables 16 to 19.

Table 16 shows the moisture content of the soil according to the number of days after planting plants under the condition of 11 wt% of drought stress (A, no drought stress), and Table 17 shows the planting under the condition of 5 wt% of drought stress (B, moderate drought stress). The results of the soil moisture content according to the number of days after are shown, and Table 18 shows the results of the soil moisture content according to the number of days after planting the plants under the condition of 3 wt% of drought stress (C, serious drought stress).

A condition Soil moisture content (wt%) according to the number of days after planting 0(day) 22(day) 32(day) E 14.00 11.24 11.30 d-1 14.00 11.66 11.64 d-2 14.00 11.79 11.69 d-3 14.00 11.86 11.87 d-4 14.00 11.95 11.92 d-5 14.00 12.24 12.31 d-6 14.00 11.93 11.85 d-7 14.00 11.95 11.89 d-8 14.00 11.85 11.77 d-9 14.00 11.86 11.75 d-10 14.00 11.91 11.85 d-11 14.00 11.92 11.89 d-12 14.00 11.87 11.84 d-13 14.00 11.89 11.85 d-14 14.00 10.20 10.25 d-15 14.00 10.25 10.26 d-16 14.00 11.20 11.35 d-17 14.00 11.35 11.37 D-1 14.00 11.66 11.59

B condition Soil moisture content (wt%) according to the number of days after planting 0(day) 22(day) 32(day) E 14.00 5.30 5.06 d-1 14.00 5.66 5.84 d-2 14.00 5.87 5.99 d-3 14.00 6.40 6.26 d-4 14.00 6.53 6.53 d-5 14.00 6.88 7.12 d-6 14.00 6.48 6.48 d-7 14.00 6.50 6.50 d-8 14.00 6.45 6.51 d-9 14.00 6.52 6.52 d-10 14.00 6.53 6.52 d-11 14.00 6.52 6.52 d-12 14.00 6.53 6.53 d-13 14.00 6.58 6.53 d-14 14.00 5.10 5.12 d-15 14.00 5.11 5.13 d-16 14.00 5.15 5.14 d-17 14.00 5.18 5.14 D-1 14.00 5.66 5.30

C condition Soil moisture content (wt%) according to the number of days after planting 0(day) 22(day) 32(day) E 14.00 3.02 5.26 d-1 14.00 3.44 5.84 d-2 14.00 3.54 5.99 d-3 14.00 3.82 6.16 d-4 14.00 4.39 6.43 d-5 14.00 4.79 7.32 d-6 14.00 4.30 5.80 d-7 14.00 4.32 5.86 d-8 14.00 4.35 5.83 d-9 14.00 4.36 5.85 d-10 14.00 4.33 5.92 d-11 14.00 4.35 5.93 d-12 14.00 4.35 5.95 d-13 14.00 4.36 5.96 d-14 14.00 4.36 4.30 d-15 14.00 4.36 4.32 d-16 14.00 3.35 4.45 d-17 14.00 3.35 4.58 D-1 14.00 3.36 5.30

Control D-1 is a super absorbent polymer and has a property of absorbing moisture up to 300 to 800 times its own weight in an instant, but as shown in Tables 16 to 18, the soil preparation composition prepared in Example 4-1 The soil treated with the immersion method maintained a significantly higher soil moisture content than the soil treated with the control E or control D-1 by the immersion method.

Example 4-3. Plants by immersion method Measurement of survival rate, plant height, and chlorophyll concentration

As in the method described in Example 4-2 above, poplar and goldworm were planted by the immersion method, and the plants were cultured under each drought stress condition (A, B, C). Plant viability, plant height, and chlorophyll concentration (SPAD unit) were analyzed. Daytime temperature 25~30℃, night temperature 15~20℃, relative humidity 35~50%RH, sunshine time 269 hours (sun days: 15 days) Plants (Poplar and Goldamcho) were cultured.

For SPAD unit (Chlorophyll concentration), the SPAD values of each of 10 leaves were measured with a SPAD meter (SPAD-502, Konica-Minolta, Tokyo, Japan), and the results are shown in Table 19. Each experimental result is expressed as an average value of 6 repeated experiments.

Table 19 shows the analysis results of poplar growth (survival rate, plant growth height and chlorophyll concentration) according to each drought stress condition (A, B, C), and Table 20 shows the 11% by weight of drought stress condition (A) (viability and plant growth height) analysis results are shown.

Index Survival rate (%) Plant height (cm) chlorophyll concentration
SPAD units drought stress conditions A (11%) B (5%) C (3%) A (11%) B (5%) C (3%) A (11%) B (5%) C (3%) E 100 100 83.3 192.4 214.2 187.6 31.5 28.2 36.6 d-1 100 100 100 201.7 212.5 205.6 34.1 37.1 40.5 d-2 100 100 100 199.3 221.3 206.8 34.6 36.5 41.8 d-3 100 100 100 204.2 223.4 212.3 40.7 38.5 42.3 d-4 100 100 100 217.2 227.8 214.6 45.3 39.7 40.2 d-5 100 100 100 218.3 229.6 215.7 46.2 41.4 41.5 d-6 100 100 100 216.5 225.4 213.5 43.2 41.5 42.5 d-7 100 100 100 216.8 227.4 213.8 45.2 43.2 42.8 d-8 100 100 100 215.5 223.7 212.6 43.8 41.8 42.0 d-9 100 100 100 215.9 225.7 214.2 44.2 44.6 43.1 d-10 100 100 100 216.2 224.8 213.1 44.5 44.8 45.2 d-11 100 100 100 216.1 227.0 215.4 45.1 45.2 41.2 d-12 100 100 100 216.5 223.5 213.9 42.8 43.4 41.9 d-13 100 100 100 215.9 226.4 213.2 43.8 42.8 40.9 d-14 85 74 66 200.2 201.2 200.8 38.5 39.2 38.7 d-15 83 75 62 202.1 200.4 201.9 39.5 36.4 38.2 d-16 75 61 55 195.4 199.4 199.2 33.4 35.6 38.7 d-17 74 66 53 192.3 200.2 201.1 33.2 39.9 40.1 D-1 100 100 100 189.5 193.4 204.5 31.5 35.9 39.9

Index E d-1 d-2 d-3 d-4 d-5 D-1 Survival rate (%) 50 75 75 75 66.7 66.8 58.3 Plant height (cm) 66.8 80.5 81.5 82.3 82.5 87.9 78.2

If the polyacrylic acid (D) solid content in the composition used for the immersion method exceeds 1% by weight, plants die due to biotoxicity and the survival rate cannot be measured, so plant growth evaluation such as plant height and SPAD could not proceed .

As shown in Tables 19 and 20, when the soil preparation composition prepared in Example 4-1 was treated with an immersion method, the survival rate of poplar and fenugreek compared to control E and control D-1, plant growth size, and / or it was confirmed that the growth of the plant was promoted by increasing the chlorophyll concentration.

Example 5. Analysis of the effect of the soil preparation composition by the soil mixing method

Example 5-1. Preparation of soil preparation composition

Soil preparation composition 1A (lysine and citric acid = 1.5:1 molar ratio included) obtained in Example 1-1 was 450 kg/ha (e-1), 900 kg/ha (e-2), 1800 kg/ha (e-3) , 3000kg/ha(e-4), 4500kg/ha(e-5) were mixed with the soil.

The soil preparation composition 1B (lysine and citric acid = 3:1 molar ratio included) obtained in Example 1-1 was mixed with the soil at a concentration of 1800 kg/ha (e-6) and 4500 kg/ha (e-7).

The soil preparation composition 1C (lysine and citric acid = 1:3 molar ratio included) obtained in Example 1-1 was mixed with the soil at a concentration of 1800 kg/ha (e-8) and 4500 kg/ha (e-9).

The soil preparation composition 1D (lysine and citric acid = 5:1 molar ratio included) obtained in Example 1-1 was mixed with the soil at a concentration of 1800 kg/ha (e-10) and 4500 kg/ha (e-11).

The soil preparation composition 1E (lysine and citric acid = 1:5 molar ratio included) obtained in Example 1-1 was mixed with the soil at a concentration of 1800 kg/ha (e-12) and 4500 kg/ha (e-13).

The soil preparation composition 1F (lysine and citric acid = 10:1 molar ratio composition) obtained in Example 1-1 was mixed with the soil at a concentration of 1800 kg/ha (e-14) and 4500 kg/ha (e-15).

The soil preparation composition 1G (lysine and citric acid = 1:10 molar ratio composition) obtained in Example 1-1 was mixed with the soil at a concentration of 1800 kg/ha (e-16) and 4500 kg/ha (e-17).

As Control D, commercially available polyacrylic acid (ASAP) was prepared. According to the guidelines described in the soil preparation, it was added to the soil in powder form and mixed with the soil at a concentration of 450 kg/ha (control group D-1). When polyacrylic acid is mixed with soil in excess of 450 kg/ha when mixed with soil, plants died due to biotoxicity and soil toxicity in this Example 5 using poplar and goldenrod.

As control E, deionized water was used as it was. Deionized water was added while adjusting the soil moisture content.

Soil preparation composition Molar ratio in soil preparation composition (Lys:CA) mixed concentration with soil e-1 Composition 1A 1.5:1 450kg/ha e-2 900kg/ha e-3 1800kg/ha e-4 3000kg/ha e-5 4500kg/ha e-6 Composition 1B 3:1 1800kg/ha e-7 4500kg/ha e-8 Composition 1C 1:3 1800kg/ha e-9 4500kg/ha e-10 Composition 1D 5:1 1800kg/ha e-11 4500kg/ha e-12 Composition 1E 1:5 1800kg/ha e-13 4500kg/ha e-14 Composition 1F 10:1 1800kg/ha e-15 4500kg/ha e-16 Composition 1G 1:10 1800kg/ha e-17 4500kg/ha Control D-1 polyacrylic acid
(ASAP) - 450kg/ha Control E deionized water - -

According to the soil mixture method (mixed with soil), using the soil mixed with the soil preparation composition at the concentration shown in Table 21, the soil moisture content measurement and plant growth analysis such as plant viability and plant growth height were performed according to the following method did.

Example 5-2. Determination of Soil Moisture Content by Soil Mixing Method

As shown in Example 5-1, the soil preparation composition (composition 1A to 1G) according to an example was well mixed with dry soil at each concentration described in Table 21 (soil mixing method), and the mixed soil was mixed with a diameter (diameter) 20cm, depth 30cm, put it in a pot, and plant Poplar (0.5 to 1 year old, 1 to 1.5m high (height)) and Caragana (1 to 2 year old 0.5m high (height)) and the soil moisture content was measured.

For each pot, 78.3% by weight of soil with an average particle size greater than 0.02 mm; average particle size 0.002mm or more - 0.02mm or less soil 14.9% by weight; And 19 kg of mixed soil in which dry soil composed of 6.8 wt% of soil with an average particle size of less than 0.002 mm and a soil preparation composition (or control) was mixed at the concentration shown in Table 21 was used. The efficacy of the soil preparation composition was evaluated using 20 pots per composition of the same concentration by the soil mixing method, and the average value of the measured results was calculated and presented as the result. The initial moisture content of the mixed soil was 14% by weight, and when the moisture content of the soil reached 3% by weight (dry stress condition) (23 days after planting), the drought stress condition was maintained for 2 days and on the 25th day after planting the plants Water was rewatered until the moisture content of the soil reached 14% by weight. When the moisture content of the soil reaches 3% by weight again (38 days after planting), the conditions are maintained for 2 days, and on the 40th day after planting the plants are sprayed with water until the moisture content of the soil reaches 14% by weight. -rewatering). In the process, soil moisture content was measured on the 23rd and 38th days after planting the plants, and the results are shown in Table 22.

Soil moisture content (wt%) according to the number of days after planting 0(day) 23(day) 38(day) E 14.00 3.01 3.92 e-1 14.00 3.79 4.82 e-2 14.00 3.97 5.38 e-3 14.00 4.12 5.21 e-4 14.00 4.65 5.43 e-5 14.00 4.97 6.89 e-6 14.00 3.89 4.89 e-7 14.00 4.75 5.98 e-8 14.00 3.92 4.95 e-9 14.00 4.85 6.08 e-10 14.00 3.94 4.93 e-11 14.00 4.78 5.99 e-12 14.00 4.02 5.01 e-13 14.00 4.95 6.21 e-14 14.00 3.57 4.23 e-15 14.00 3.58 4.25 e-16 14.00 3.64 4.38 e-17 14.00 3.65 4.44 D-1 14.00 3.67 4.57

As shown in Table 22, when the soil preparation composition prepared in Example 5-1 was mixed with the soil, the soil moisture content was increased under the drought stress condition compared to the control E and the control D-1, and the mixed concentration with the soil Soil moisture content increased with increasing .

Example 5-3. Plant Growth Analysis by Soil Mixing Method

As a soil mixing method (mixed with soil), the soil preparation composition prepared in Example 5-1 is sprayed on the soil at each concentration, and then the soil and the composition on the soil surface are mixed with a tractor, and then the wood (Poplar (Poplar) ) and gold dam grass (Caragana)) were planted. Soil preparation composition (or control) and dry soil (78.3 wt% of soil with an average particle size of more than 0.02 mm; 14.9 wt% of soil with an average particle size of 0.002 mm or more - 0.02 mm or less; and 6.8 wt% of soil with an average particle size of less than 0.002 mm) By mixing, plant growth analysis was performed according to the soil mixing method. 50 trees were planted based on the same concentration of the composition, and each result was averaged and presented as a result value. Plant growth evaluation was conducted in dry climate field conditions (FIG. 2) with an average annual precipitation of 365.7 mm, and the survival rate and plant growth of each plant were analyzed 5 months after planting of poplar and 5 months after planting of Guldam plant.

In the case of poplar, a 1.5m-sized individual with a similar thickness (diameter) of each individual is planted, and after growth, the diameter of the trunk (Diameter at 0.5m above ground) at the same location (0.5m above the ground) was measured, and the number of new shoots and new shoots was measured because the plants were planted without stems and leaves at the time of planting. Therefore, the width of leaves was measured and used as an indicator of plant growth.

In the case of Golddamer plant, the plant height was measured, and since Golddamcho is not a tree that grows upright like poplar, the Crown Diameter was measured and used as a plant growth index.

Table 23 shows the survival rate (%) of the poplar tree and the cypress tree, Table 24 shows the result of analyzing the growth of the poplar tree, and Table 25 shows the result of analyzing the growth of the gonzo tree.

Survival rate (%) poplar Goldhamcho (Caragana) E 81 72 e-1 82 74 e-2 82 80 e-3 90 85 e-4 92 84 e-5 98 82 e-6 88 82 e-7 93 80 e-8 87 81 e-9 92 78 e-10 87 83 e-11 93 79 e-12 86 81 e-13 91 74 e-14 75 59 e-15 73 60 e-16 74 52 e-17 74 55 D-1 78 64

poplar Diameter at 0.5m above ground Number of new shoot Length of leaves(cm) Width of leaves(cm) E 1.2 5.9 6.1 5.7 e-2 1.3 8.0 9.0 8.1 e-3 1.6 6.2 9.0 8.2 e-5 1.5 6.1 8.2 8.1 D-1 1.2 5.8 7.6 6.2

Golddamcho Crown Diameter (cm) Plant height (cm) E 80.0 66 e-2 82.0 79 e-3 81.0 83 e-5 78.0 72 D-1 73.0 71

As shown in Table 23, when evaluating the plant growth in the soil mixed with the soil preparation composition as in the concentration described in Example 5-1, the growth rate of plants (poplars and dandelions) than in the soil mixed with control D or control E this increased. As shown in Table 24, when evaluating the growth of poplar in the soil mixed with the soil preparation composition at the concentration described in Example 5-1, the trunk diameter, number of young branches and leaves than in the soil mixed with Control D or Control E and leaf area increased. As shown in Table 25, when evaluating the growth of poplar in the soil mixed with the soil preparation composition as in the concentration described in Example 5-1, the plant growth height and crown diameter were higher than in the soil mixed with control D or control E. increased. In the case of e-5, the crown diameter seems to be lower than that of the control E, but the growth size (height) of the plant itself is larger than that of the control E, so the number of branches and leaves of the dandelion plant is higher than that of the control E. It was found to increase the growth of gold dammosa than E or D-1.

Control D-1 (polyacrylic acid (D)) was used in excess of 450 kg/ha, and more than half of the trees died compared to control E due to toxicity, so plant growth evaluation could not proceed.

Example 6. Analysis of the plant growth promotion effect of the soil preparation composition according to the mixing molar ratio of lysine and citric acid under dry conditions

Example 6-1. Preparation of soil preparation composition

The soil preparation composition 1A (lysine and citric acid = 1.5:1 molar ratio) obtained in Example 1-1 was mixed with 300 kg/ha (f-1), 450 kg/ha (f-2), 600 kg/ha (f-3), It was mixed with soil at a concentration of 900 kg/ha (f-4), 1200 kg/ha (f-5), and 2400 kg/ha (f-6).

The soil preparation composition 1B (lysine and citric acid = 3:1 molar ratio) obtained in Example 1-1 was mixed with the soil at a concentration of 600 kg/ha (f-7) and 900 kg/ha (f-8).

The soil preparation composition 1C (lysine and citric acid = 1:3 molar ratio) obtained in Example 1-1 was mixed with the soil at a concentration of 600 kg/ha (f-9) and 900 kg/ha (f-10).

The soil preparation composition 1D (lysine and citric acid = 5:1 molar ratio) obtained in Example 1-1 was mixed with the soil at a concentration of 600 kg/ha (f-11) and 900 kg/ha (f-12).

The soil preparation composition 1E (lysine and citric acid = 1:5 molar ratio) obtained in Example 1-1 was mixed with the soil at a concentration of 600 kg/ha (f-13) and 900 kg/ha (f-14).

The soil preparation composition 1F (lysine and citric acid = 10:1 molar ratio) obtained in Example 1-1 was mixed with the soil at a concentration of 600 kg/ha (f-15) and 900 kg/ha (f-16).

The soil preparation composition 1G (lysine and citric acid = 1:10 molar ratio) obtained in Example 1-1 was mixed with the soil at a concentration of 600 kg/ha (f-17) and 900 kg/ha (f-18).

As Control D, commercially available polyacrylic acid (ASAP) was prepared. According to the guidelines described in the soil preparation, it was mixed with the soil at a concentration of 450 kg/ha (control D-1), or 600 kg/ha (control D-2) in powder form and used.

As control E, deionized water was used as it was.

Soil preparation composition Molar ratio (Lys:CA) mixed concentration with soil f-1 Composition 1A 1.5:1 300kg/ha f-2 450kg/ha f-3 600kg/ha f-4 900kg/ha f-5 1200kg/ha f-6 2400kg/ha f-7 Composition 1B 3:1 600kg/ha f-8 900kg/ha f-9 Composition 1C 1:3 600kg/ha f-10 900kg/ha f-11 Composition 1D 5:1 600kg/ha f-12 900kg/ha f-13 Composition 1E 1:5 600kg/ha f-14 900kg/ha f-15 Composition 1F 10:1 600kg/ha f-16 900kg/ha f-17 Composition 1G 1:10 600kg/ha f-18 900kg/ha Control D-1 polyacrylic acid - 450kg/ha Control D-2 polyacrylic acid - 600kg/ha Control E deionized water - -

Plant growth analysis in dry conditions (3 types of dry soil and 3 types of drought stress conditions applied) at the time of treatment of each soil preparation composition was evaluated according to the following method.

Example 6-2. Plant growth analysis under dry conditions

Example 6-2-1. Analysis of maize growth by soil mixing method

As in Example 6-1, the soil preparation composition (composition 1A to 1G) according to an example was mixed with dry soil at each concentration described in Table 26 (soil mixing method), and the mixed soil was mixed with a potted plant having a surface area of 452 cm ² Then plant corn (Maize, Yuhe863) at 28~33℃ (day), 20~25℃ (night) (sun hour 269 hours (sun days 20 days) ~ 305 hours (sun days 15 days) ), the plants were cultured under the conditions of As the base fertilizer in the soil, N, P, K are 0.138g/kg nitrogen (N) (Urea, granule, Nitrogen content 46%), 0.156g/kg P ₂ O ₅ (superphosphate, Granule, Phosphorus content 20% compared to dry soil) ), 0.18 g/kg K ₂ O (Potassium chloride, powder, Potassium content 60%) was added.

Soil a (41.5% by weight of soil with an average particle size of more than 0.02 mm; 30.2% by weight of soil with an average particle size of 0.002 mm or more - 0.02 mm or less; 28.3% by weight of soil with an average particle size of less than 0.002 mm), b soil (soil with an average particle size of more than 0.02 mm 59.6%) Weight %; average particle size of 0.002mm or more - 0.02mm or less 24.7% by weight; 15.7% by weight of soil with an average particle size of less than 0.002mm), c soil (78.3% by weight of soil with an average particle size of more than 0.02mm; soil with an average particle size of 0.002mm or more - 0.02mm or less) 3 types of dry soil composed of 14.9% by weight; 6.8% by weight of soil with an average particle size of less than 0.002mm) were used by 8 kg each. The components of the mixed soil a, b, and c are shown in Table 27 below.

More than 0.02mm 0.02 or less to 0.002 mm or more less than 0.002mm a soil 41.50% by weight 30.20% by weight 28.30% by weight b Soil 59.60% by weight 24.70% by weight 15.70% by weight c soil 78.30% by weight 14.90% by weight 6.80% by weight

For the soil mixing method, efficacy evaluation was conducted by averaging 12 pollen per composition at the same concentration. As drought stress conditions, (1) a soil has a soil moisture content of 15.2% by weight (A, no drought stress) and a soil moisture content of 4.3% by weight (C, serious drought stress), (2) b soil has a soil moisture content Under conditions of 11.6% by weight (A, no drought stress) and 3.3% by weight of soil moisture content (C, serious drought stress), (3) c soil has a soil moisture content of 8.7% by weight (A, no drought stress), Efficacy evaluation was carried out under 2.5 wt% conditions (C, serious drought stress).

a Condition A of the soil: 1) When the moisture content in the soil reaches 15.2% by weight under the drought stress condition from 19.5% by weight (37 days after planting), after maintaining the drought stress condition for 3 days, water to reach 19.5% by weight. After spraying (40 days after planting) and 2) when the drought stress condition reaches 15.2 wt% again (46 days after planting), the drought stress condition is maintained for 3 days, and then water is sprayed to reach 19.5 wt% (49 days after planting) ) was done.

a Soil C condition: 1) When the moisture content in the soil reaches 4.3% by weight from 19.5% by weight to 4.3% by weight (37 days after planting), after maintaining the drought stress condition for 3 days, water to reach 15.2% by weight. After spraying (40 days after planting) and 2) reaching drought stress conditions again (46 days after planting), the drought stress condition is maintained at 4.3 wt% for 3 days, and then water is sprayed to reach 15.2 wt% (49 days after planting) ) was done.

b Condition A of soil: 1) When the moisture content in the soil reaches 11.6% by weight from 14.9% by weight to 11.6% by weight (37 days after planting), after maintaining the drought stress condition for 3 days, water to reach 14.9% by weight. After spraying (40th day after planting), 2) When the drought stress condition reaches 11.6% by weight (46 days after planting), the drought stress condition is maintained for 3 days, and then water is sprayed to reach 14.9% by weight (49 after planting) first).

b Soil C condition: 1) When the moisture content in the soil reaches 3.3% by weight from 14.9% by weight to 3.3% by weight (37 days after planting), after maintaining the drought stress condition for 3 days, water to reach 11.6% by weight. After spraying (40 days after planting) and 2) when the drought stress condition reaches 3.3 wt% again (46 days after planting), the drought stress condition is maintained for 3 days, and then water is sprayed to reach 11.6 wt% (49 days after planting) ) was done.

c Condition A of soil: 1) When the moisture content in the soil reaches 8.7% by weight under drought stress conditions from 11.2% by weight (37 days after planting), after maintaining the drought stress condition for 3 days, water to reach 11.2% by weight. When spraying (40 days after planting and 2) reaches drought stress condition of 8.7 wt% again (46 days after planting), the drought stress condition is maintained for 3 days, and then water is sprayed to reach 11.2 wt% (49 days after planting) did.

c Condition C of the soil: 1) When the moisture content in the soil reaches 2.5% by weight under the drought stress condition from 11.2% by weight (37 days after planting), after maintaining the drought stress condition for 3 days, water to reach 8.7% by weight. After spraying (40 days after planting) and 2) when the drought stress condition reaches 2.5 wt% again (46 days after planting), the drought stress condition is maintained for 3 days, and then water is sprayed to reach 8.7 wt% (49 days after planting) )do.

In each of the above conditions, the moisture content in the soil reaches the moisture content wt% of each drought stress condition, and then sprays water to rewater, and when each drought stress condition is reached, plants (corn) during two cycles of drought-rewatering. Growth analysis was performed to analyze plant growth on the 50th day after planting (plant growth height, chlorophyll concentration, dry weight), and the results are shown in Tables 29 to 34 below.

Table 28 below shows the drought stress conditions (soil moisture content) in each soil.

A condition
(no drought stress) C condition
(serious drought stress) a soil 15.2% by weight 4.3% by weight b soil 11.6% by weight 3.3% by weight c soil 8.7% by weight 2.5% by weight

SPAD unit was measured in the same manner as in Example 4, and the amount of corn biomass was measured 50 days after sowing maize seeds, each stem and root of a crop generated in 6 pots per the same concentration of soil preparation composition. After harvesting and drying at 70° C. for 72 hours, the dry weight (g) was measured.

Tables 29 and 30 show the analysis of corn growth in soil a (plant growth height, chlorophyll concentration, dry weight), and Table 31 and Table 32 show the analysis of corn growth in soil b (plant growth height, chlorophyll concentration, dry weight). ), and Tables 33 and 34 show the analysis of corn growth in soil c (plant growth height, chlorophyll concentration, dry weight).

a soil plant height (cm) SPAD unit drought stress conditions A (15.2%) C (4.3%) A (15.2%) C (4.3%) E 0 105.7 89.4 30.4 25.9 f-1 300 119.7 92.4 31.6 27.6 f-3 600 121.8 105.8 35.8 31.4 f-4 900 132.4 108.3 36.2 32.6 f-7 600 120.3 103.4 33.1 30.0 f-8 900 126.3 106.6 33.2 30.2 f-9 600 119.8 102.3 32.1 30.2 f-10 900 125.6 102.5 32.8 30.2 f-11 600 120.6 103.9 33.9 30.4 f-12 900 126.7 106.1 34.5 30.5 f-13 600 119.6 102.1 33.2 30.1 f-14 900 125.1 102.1 34.1 30.8 f-15 600 115.0 91.2 30.1 26.2 f-16 900 116.1 91.6 30.4 26.1 f-17 600 115.3 90.8 30.2 26.3 f-18 900 115.4 90.1 30.3 26.4 D-2 600 119.3 91.6 29.7 26.8

a soil Shoot dry weight (g) Root dry weight (g) Total dry weight (g) drought stress conditions A (15.2%) C (4.3%) A (15.2%) C (4.3%) A (15.2%) C (4.3%) E 0 17.8 13.8 9.3 7.6 27.1 21.4 f-1 300 29.5 14.7 10.6 9.4 31.1 24.1 f-3 600 25.4 16.9 11.2 9.8 36.6 26.7 f-4 900 25.7 17.2 11.5 9.9 37.2 27.1 f-5 1200 23.7 14.8 10.8 8.6 34.5 23.4 f-7 600 24.3 15.9 10.9 9.5 35.2 25.4 f-8 900 24.5 16.1 11.0 9.6 35.5 25.7 f-9 600 24.3 16.0 11.1 9.5 35.4 25.5 f-10 900 24.4 16.1 11.1 9.5 35.5 25.6 f-11 600 25.1 16.2 10.8 9.6 35.9 25.8 f-12 900 25.2 16.3 11.2 9.6 36.4 25.9 f-13 600 24.9 15.9 10.9 9.4 35.8 25.3 f-14 900 25.0 16.1 11.1 9.4 36.1 25.5 f-15 600 18.1 14.3 10.2 8.5 28.3 22.8 f-16 900 18.2 14.1 10.1 8.6 28.3 22.7 f-17 600 18.3 13.2 10.2 8.4 28.5 21.6 f-18 900 18.2 13.9 10.5 8.5 28.7 22.4 D-2 600 18.1 9.4 9.3 7.3 27.4 16.7

b soil plant height (cm) SPAD unit drought stress conditions A (11.6%) C (3.3%) A (11.6%) C (3.3%) E 0 85.4 84.5 23.4 22.8 f-1 300 95.8 92.7 26.4 25.9 f-3 600 99.3 95.3 28.9 26.9 f-4 900 97.3 94.6 28.6 26.2 f-7 600 98.5 93.2 27.5 25.9 f-9 600 98.9 93.1 27.6 26.2 f-11 600 98.4 93.5 27.2 26.1 f-13 600 98.6 93.2 27.3 26.3 f-15 600 85.6 85.3 23.5 23.1 f-17 600 85.9 85.2 23.5 23.2 D-2 600 91.6 88.2 26.2 25.1

b soil Shoot dry weight (g) Root dry weight (g) Total dry weight (g) drought stress conditions A (11.6%) C (3.3%) A (11.6%) C (3.3%) A (11.6%) C (3.3%) E 0 13.6 9.1 5.1 3.8 18.7 12.9 f-1 300 16.5 11.3 6.4 6.0 22.9 15.3 f-3 600 17.7 12.1 8.1 7.1 25.8 17.3 f-4 900 16.8 11.9 7.4 6.1 24.2 19.2 f-7 600 16.2 11.8 7.5 6.4 23.7 18.2 f-9 600 16.3 11.9 7.5 6.8 23.8 18.7 f-11 600 16.2 11.7 7.6 6.5 23.8 18.2 f-13 600 16.3 11.9 7.6 6.7 23.9 18.6 f-15 600 14.2 10.1 5.2 4.2 19.4 14.3 f-17 600 14.1 10.2 5.2 4.1 19.3 14.3 D-2 600 15.9 10.2 6.2 5.4 22.1 15.0

c soil plant height (cm) SPAD unit drought stress conditions A (8.7%) C (2.5%) A (8.7%) C (2.5%) E 0 102.9 80.3 27.6 26.2 f-1 300 106.7 88.4 31.6 28.6 f-3 600 113.5 103.4 34.8 33.1 f-4 900 112.1 103.9 35.9 34.5 f-5 1200 103.0 92.4 28.8 27.3 f-7 600 110.2 102.4 34.5 33.1 f-8 900 111.0 102.5 34.2 33.1 f-9 600 110.4 102.1 34.6 32.8 f-10 900 110.6 102.0 34.9 32.9 f-11 600 110.1 102.4 33.9 32.4 f-12 900 110.2 102.9 33.8 32.5 f-13 600 110.8 101.4 34.2 32.9 f-14 900 110.2 101.9 34.1 32.5 f-15 600 103.2 90.2 31.8 28.2 f-16 900 103.1 90.1 31.8 28.6 f-17 600 103.5 91.2 32.1 29.1 f-18 900 103.6 90.8 31.9 28.6 D-2 600 73.4 71.6 22.4 21.1

c soil Shoot dry weight (g) Root dry weight (g) Total dry weight (g) drought stress conditions A (8.7%) C (2.5%) A (8.7%) C (2.5%) A (8.7%) C (2.5%) E 0 15.3 12.4 5.6 4.7 20.9 17.1 f-1 300 15.5 12.8 8.9 5.1 21.4 17.9 f-3 600 18.5 13.4 8.4 7.4 26.9 20.8 f-4 900 18.6 14.6 9.6 7.9 28.2 22.5 f-5 1200 17.2 13.8 10.8 8.8 28.0 22.6 f-7 600 17.9 13.1 7.9 7.2 25.8 20.3 f-8 900 17.6 13.1 8.5 7.3 26.1 20.4 f-9 600 17.5 13.2 7.8 7.1 25.3 20.3 f-10 900 17.4 13.3 8.4 7.2 25.8 20.5 f-11 600 17.6 12.8 7.9 6.8 25.5 19.6 f-12 900 17.9 12.9 8.6 6.9 26.5 19.8 f-13 600 17.2 12.8 7.1 6.7 24.3 19.5 f-14 900 18.1 12.7 8.3 6.8 26.4 19.5 f-15 600 16.2 12.5 6.1 5.2 22.3 17.7 f-16 900 16.1 13.0 6.0 5.3 22.1 18.3 f-17 600 16.2 12.6 6.2 5.1 22.4 17.7 f-18 900 16.3 13.2 6.3 5.6 22.6 18.8 D-2 600 11.8 10.2 6.7 4.9 18.5 15.1

As shown in Tables 29 to 34, when the soil preparation composition according to an example is applied to the soil, the plant size, chlorophyll concentration (SPAD concentration), and biomass (above part) of corn under dry conditions compared to controls E and D-2 stem dry weight, root dry weight, total dry weight) were increased. As the solid content in the soil preparation composition increased, the plant growth promoting effect of the composition increased.

Experiments were carried out in Tables 29 to 34, and soil pH and residual electrical conductivity in the soil were measured after sowing corn grown 5 months after sowing and harvesting 5 months later using soil a, soil b, and soil c (soil mixing method). The results are shown in Table 35 below. After corn was harvested (5 months after sowing), 10 g of each dried soil was passed through a 1 mm sieve, mixed with 50 ml of water, shaken and stirred for 5 minutes, filtered through filter paper, and measured with a conductivity meter (Mettler Toledo). Soil electrical conductivity was measured. Soil electrical conductivity is shown in a table as the result of repeated experiments three times.

soil Index pH Conductivity (dS/m) a soil E 7.13 0.21 f-1 7.08 0.22 f-3 7.02 0.22 f-4 6.95 0.22 f-5 6.82 0.23 b soil E 7.55 0.17 f-1 7.5 0.18 f-3 7.42 0.18 f-4 7.38 0.17 c soil E 8.03 0.16 f-1 7.96 0.20 f-3 7.91 0.22 f-4 7.88 0.23 f-5 7.83 0.24

As shown in Table 35, when the soil preparation composition according to an example was treated, the pH of the soil was transferred to neutral than that of the deionized water E treatment, and the electrical conductivity was in a range that does not affect saline soil formation. It was found that soil fertility was improved by increasing the amount of organic matter available to plants in the soil. When the basicity of the soil increases, nutrients such as phosphorus, zinc, magnesium, calcium, and boron in the soil become hardened, which prevents plants from using them, and may cause growth problems such as plant death. When the soil preparation composition according to an example is applied, nutrients and moisture content in the soil are increased to increase the dissolution of nutrients, and by supplying nutrients required by plants, electrical conductivity in the soil is improved.

Example 6-2-2. Analysis of ryegrass and alfalfa growth according to soil mixing method

As in Example 6-1, the soil preparation composition (compositions 1A to 1G) according to an example was mixed with dry soil at each concentration described in Table 26 (soil mixing method) and placed in a pot with a surface area of 78 cm ² , and then plants Sow ryegrass (Secale Cerale, Dongmu 70) and alfalfa (ryegrass; Medicago Sativa), respectively, and then 28~33℃(day), 20~25℃(night)(sun hour 202 hours (sun days 16 days) It was cultured under conditions of ~282 hours (sun days 28 days). As the base fertilizer in the soil, N, P, and K are 0.1g/kg nitrogen (N) concentration (Urea, granule, Nitrogen content 46%), 0.04g/kg P ₂ O ₅ concentration (superphosphate, granule, Phosphorus content) compared to dry soil 20%), 0.15 g/kg K ₂ O concentration (Potassium chloride, powder, Potassium content 60%) was added.

For dry soil, 1.2 kg of dry soil composed of c soil (78.3 wt% of soil with an average particle size of more than 0.02 mm; 14.9% of soil with an average particle size of 0.002 mm or more - 0.02 mm or less; and 6.8% of soil with an average particle size of less than 0.002 mm; Table 27) was used. did. For the soil mixing method, efficacy evaluation was conducted by averaging 12 pollen per composition at the same concentration. For the soil c, the efficacy of the soil preparation composition was evaluated under conditions of 11 wt% soil moisture content (A, no drought stress) and 3 wt% (C, serious drought stress) as drought stress conditions. In the first cycle, when the moisture content in the soil reaches each drought stress condition (on the 35th day after planting), it is maintained under the drought stress condition for 2 days, and then water is sprayed to reach the moisture content of 14% by weight (37th day after planting) and rewatered And when each drought condition is reached again in the second cycle (after planting, on the 45th day), the drought stress condition is maintained for 2 days, and then water is sprayed (47 days after planting) to reach 14% by weight in the soil and rewatered. Again, growth analysis of ryegrass or alfalfa was performed during two cycles of drought-rewatering under conditions that reached each drought condition. The amount of biomass of ryegrass or alfalfa is measured by dry weight after harvesting each stem and root of biomass generated from 6 pots treated with the same concentration 50 days after sowing of each seed, and drying at 70° C. for 72 hours. Thus, the results are shown in Table 36.

of ryegrass
Total dry weight (g) alfalfa
Total dry weight (g) composition density A (11%) C (3%) A (11%) C (3%) E 0 0.67 0.47 0.49 0.34 f-1 300 0.78 0.65 0.66 0.45 f-3 600 0.83 0.69 0.75 0.52 f-4 900 0.80 0.68 0.68 0.49 D-2 600 0.77 0.63 0.65 0.44

Example 6-3. Analysis of plant growth according to the soil spray method in a greenhouse

Efficacy evaluation by spray on the soil surface was performed on dry soil (a, b, c; Table 27) except that the soil preparation composition was sprayed with the composition and concentration of Table 37 below, respectively. Plant (corn, ryegrass, and alfalfa) growth analysis was performed under conditions and methods similar to Example 6-2-1 (growth analysis of maize), Example 6-2-2 (analysis of growth of ryegrass and alfalfa) did.

Soil preparation composition Molar ratio in soil preparation composition (Lys:CA) Soil spray concentration f-1 Composition 1A 1.5:1 300kg/ha f-3 600kg/ha f-4 900kg/ha f-5 1200kg/ha f-7 Composition 1B 3:1 600kg/ha f-8 900kg/ha f-9 Composition 1C 1:3 600kg/ha f-10 900kg/ha f-11 Composition 1D 5:1 600kg/ha f-12 900kg/ha f-13 Composition 1E 1:5 600kg/ha f-14 900kg/ha f-15 Composition 1F 10:1 600kg/ha f-16 900kg/ha f-17 Composition 1G 1:10 600kg/ha f-18 900kg/ha Control D-2 polyacrylic acid - 600kg/ha Control E deionized water - -

Tables 38 and 39 show the biomass analysis results for corn in soil a (soil application method), and Tables 40 and 41 show the biomass analysis results for corn in soil b (soil application method). and Table 42 and Table 43 show the results of biomass analysis for corn in soil c (soil application method).

a soil plant height (cm) SPAD unit Cooking agent concentration A (8.7%) C (2.5%) A (8.7%) C (2.5%) E 0 109.5 90.5 33.4 31.4 f-1 300 122.5 102.4 35.9 36.5 f-3 600 133.8 107.8 41.3 37.2 f-4 900 139.1 109.4 42.8 39.4 f-5 1200 129.3 104.1 37.0 36.1 f-7 600 128.9 105.2 38.5 35.6 f-8 900 130.2 105.1 38.6 35.6 f-9 600 127.5 106.3 37.8 35.4 f-10 900 131.2 106.8 37.9 35.9 f-11 600 129.5 105.0 38.1 35.2 f-12 900 132.8 105.9 38.6 35.3 f-13 600 127.4 105.1 36.8 34.9 f-14 900 131.0 105.6 37.9 35.2 f-15 600 115.2 91.2 33.2 31.5 f-16 900 116.8 91.3 33.8 32.4 f-17 600 115.2 92.1 34.2 33.8 f-18 900 116.7 93.5 35.1 33.6 D-2 600 115.4 93.7 35.1 34.5

a soil Shoot dry weight (g) Root dry weight (g) Total dry weight (g) Cooking agent concentration A (8.7%) C (2.5%) A (8.7%) C (2.5%) A (8.7%) C (2.5%) E 0 19.3 16.2 9.7 6.1 29.0 22.3 f-1 300 20.3 18.6 11.4 6.6 31.7 25.2 f-3 600 22.0 18.5 12.8 9.6 34.8 28.1 f-4 900 21.2 19.3 13.7 9.8 34.9 29.1 f-5 1200 21.8 18.7 12.4 9.1 34.2 27.8 f-7 600 21.5 17.5 12.1 8.9 33.6 26.4 f-8 900 21.6 17.6 12.3 9.0 33.9 26.6 f-9 600 22.1 17.5 12.1 8.8 34.2 26.3 f-10 900 22.0 17.5 12.4 8.9 34.4 26.4 f-11 600 21.4 18.1 12.1 9.1 33.5 27.2 f-12 900 21.6 17.9 12.2 9.2 33.8 27.1 f-13 600 22.0 17.3 12.5 8.8 34.5 26.1 f-14 900 21.8 17.4 12.6 8.4 34.4 25.8 f-15 600 20.3 16.5 10.1 7.1 30.4 23.6 f-16 900 20.4 16.2 10.0 6.9 30.4 23.1 f-17 600 20.1 16.2 10.1 7.1 30.2 23.3 f-18 900 20.6 16.8 10.3 7.3 30.9 24.1 D-2 600 18.8 14.3 9.3 5.8 28.1 20.1

b Soil plant height (cm) SPAD unit Cooking agent concentration A (11.6%) C (3.3%) A (11.6%) C (3.3%) E 0 90.5 88.4 24.4 23.5 f-3 600 107.8 101.8 28.4 27.9 f-4 900 100.4 97.6 27.5 26.5 f-7 600 106.2 99.8 27.1 26.8 f-9 600 105.6 99.9 27.3 26.8 f-11 600 105.8 98.7 27.5 26.4 f-13 600 106.2 99.0 27.6 26.8 f-15 600 100.1 97.2 25.4 24.3 f-17 600 101.2 96.8 26.1 24.0 D-2 600 99.7 96.9 26.2 23.6

b Soil Shoot dry weight (g) Root dry weight (g) Total dry weight (g) Cooking agent concentration A (11.6%) C (3.3%) A (11.6%) C (3.3%) A (11.6%) C (3.3%) E 0 13.7 8.4 6.4 3.5 20.1 11.9 f-1 300 15.7 10.4 7.9 6.5 23.6 16.9 f-3 600 18.5 12.1 8.2 7.6 26.7 19.7 f-4 900 16.1 10.9 8.1 7.1 24.2 18.0 f-7 600 17.1 11.0 7.8 7.1 24.9 18.1 f-9 600 17.2 11.2 7.9 7.5 25.1 18.7 f-11 600 17.3 11.0 7.8 7.2 25.1 18.2 f-13 600 17.2 11.3 7.9 7.4 25.1 18.7 f-15 600 14.3 9.2 6.8 5.9 21.1 15.1 f-17 600 14.9 9.3 6.9 6.1 21.8 15.4 D-2 600 14.9 8.9 6.5 5.6 21.4 14.5

c soil plant height (cm) SPAD unit Cooking agent concentration A (8.7%) C (2.5%) A (8.7%) C (2.5%) E 0 88.4 78.9 24.6 22.4 f-1 300 108.4 99.7 34.3 33.2 f-3 600 124.6 110.5 36.5 33.9 f-4 900 122.4 107.5 36.1 35.2 f-5 1200 119.5 102.5 35.4 34.3 f-7 600 122.3 107.5 35.0 32.1 f-8 900 121.3 105.1 35.2 34.1 f-9 600 122.4 107.6 35.3 32.0 f-10 900 121.2 105.3 35.1 34.2 f-11 600 122.1 107.1 35.2 33.0 f-12 900 120.8 105.2 35.6 34.1 f-13 600 121.9 107.3 35.8 32.9 f-14 900 121.6 105.2 35.9 34.5 f-15 600 100.2 90.8 30.1 25.0 f-16 900 100.5 91.5 30.5 25.8 f-17 600 99.5 90.9 30.2 25.5 f-18 900 100.3 91.6 30.1 25.4 D-2 600 81.3 74.6 22.1 20.4

c soil Shoot dry weight (g) Root dry weight (g) Total dry weight (g) Cooking agent concentration A (8.7%) C (2.5%) A (8.7%) C (2.5%) A (8.7%) C (2.5%) E 0 13.5 9.4 6.4 5.5 19.9 14.9 f-1 300 15.8 11.8 9.6 6.0 23.4 17.8 f-3 600 18.6 14.8 9.1 7.6 27.7 22.4 f-4 900 17.8 14.4 9.5 6.9 27.3 21.3 f-7 600 16.3 14.0 8.8 7.0 25.1 21.0 f-8 900 15.8 13.8 9.0 6.4 24.8 20.2 f-9 600 16.3 14.1 8.5 7.2 24.8 21.3 f-10 900 15.6 13.9 9.0 6.5 24.6 20.4 f-11 600 16.8 14.1 8.6 7.2 25.4 21.3 f-12 900 15.6 13.4 9.0 6.0 24.6 19.4 f-13 600 16.4 14.2 8.4 7.4 24.8 21.6 f-14 900 15.4 13.5 9.1 6.9 24.5 20.4 f-15 600 14.1 10.2 7.0 5.6 21.1 15.8 f-16 900 14.2 10.2 7.1 5.8 21.3 16.0 f-17 600 14.2 10.2 6.9 5.9 21.1 16.1 f-18 900 14.3 10.5 7.1 6.1 21.4 16.6 D-2 600 11.9 8.2 8.3 5.6 20.2 13.8

Table 44 shows the results of analyzing dry yields of ryegrass and alfalfa in soil c (soil application method).

c soil of ryegrass
Total dry weight (g) alfalfa
Total dry weight (g) Cooking agent concentration A (11%) C (3%) A (11%) C (3%) E 0 0.38 0.24 0.35 0.20 f-1 300 0.46 0.38 0.53 0.42 f-3 600 0.51 0.43 0.56 0.46 f-4 900 0.51 0.43 0.58 0.47 D-2 600 0.43 0.37 0.49 0.41

Example 6-4. Analysis of plant growth according to soil mixing method in the field

After mixing the soil preparation composition according to an example with dry soil for each concentration described in Table 26 and spraying it on 3 plots of an area of 20 m ² of soil, the composition was mixed with the soil on the soil surface with a tractor (soil mixing method) , maize, potato, ryegrass or alfalfa (Alfalfa, Medicago Sativa) were planted. Corn, ryegrass, and alfalfa were sown as seeds, and potatoes were sown as seed potatoes. Urea (Urea, granule, Nitrogen content 46%) at a concentration of 180 kg/ha as a nitrogen source in the soil for corn cultivation was used in the growth stages of four leaf, honking period, and tasseling stage, respectively. As an N and P source in the soil for potato cultivation, diammonium phosphate was used at a concentration of 225 kg/ha before sowing potatoes. Base fertilizer in the soil for ryegrass and alfalfa was not treated.

As dry soil, soil c (78.3% by weight of soil with an average particle size of more than 0.02 mm, 14.9% by weight of soil with an average particle size of 0.02 mm or more - 0.002 mm or less, and 6.8% by weight of soil with an average particle size of less than 0.002 mm) was used. Plants (corn, potato, rice, and alfalfa) were grown in dry climate field conditions (Fig. 2) with an annual average temperature of 8.3 °C for 50 years and an average annual precipitation of 365.7 mm for 50 years, and corn was harvested 158 days after sowing. do. On day 135, potatoes, ryegrass, and alfalfa were harvested and the dry mass yield of each was analyzed. The monthly rainfall and temperature of the area where the field experiment was performed are shown in FIG. 2 .

Table 45 shows the yield of maize grown in field conditions (soil mixing method, c soil). As shown in Table 45, the production amount of corn grown in the soil to which the soil preparation composition prepared in Example 6-1 was applied was increased compared to Controls E and D-1.

(corn) Grain Yield (kg/ha) E 0 11064 f-2 450 12378 f-5 1200 13232 f-6 2400 12949 D-1 450 11533

Table 46 shows the yield of potatoes grown under field conditions (soil mixing method, c soil). As shown in Table 46, compared to Controls E and D-1, the total yield of potatoes grown in the soil to which the soil preparation composition prepared in Example 6-1 was applied increased. The proportion of large potatoes was further increased.

(potato) Total Yield (kg/ha) >200g Yield (kg/ha) 80~200g Yield (kg/ha) <80g Yield (kg/ha) E 0 13301 5313 6252 1734 f-1 300 14136 5593 6208 2335 f-3 600 16520 8685 6694 1141 f-5 1200 17703 8812 6356 2534 D-1 450 14133 5862 5720 2550

Table 47 shows the results of analyzing the dry weight of ryegrass and alfalfa grown in field conditions (soil mixing method, c soil). As shown in Table 47, the dry weight of ryegrass and alfalfa grown in the soil to which the soil preparation composition prepared in Example 6-1 was applied was increased compared to Controls E and D-1.

Index alfalfa
Total dry weight (g) of ryegrass
Total dry weight (g) E 0 2760 1154 f-2 450 3800 1708 f-5 1200 3807 3084 f-6 2400 3803 3971 D-1 450 2990 708

As shown in Tables 45 to 47, the soil preparation composition according to an example contained biomass (crop of corn, potato, ryegrass, and alfalfa) compared to control E (deionized water), and controls D-1 and D-2. Production amount or dry weight of plants) significantly increased the yield, and the effect was increased as the treatment concentration of the composition increased.

Example 7. Confirmation of fertilizer effect enhancement activity of soil preparation composition

As described in Example 6-1, the soil preparation composition was mixed with the soil (f-1 (300 kg/ha), f-3 (600 kg/ha), and f-4 (900 kg/ha)) and commercially available Urea were added at concentrations of 20 kg / ha, 40 kg / ha, and 60 kg / ha and mixed in the soil to obtain the concentration combinations shown in Table 48 below. As other fertilizer components, 150 kg/ha (calcium superphosphate, 15% phosphorus) as P source and 120 kg/ha (potassium chloride, 60% potassium) as K source were used. As the soil, soil c (78.3% by weight of soil with an average particle size of more than 0.02 mm; 14.9% by weight of soil with an average particle size of 0.002 mm or more - 0.02 mm or less; 6.8% by weight of soil with an average particle size of less than 0.002 mm) was used.

8 kg of mixed soil containing the soil preparation composition and fertilizer components prepared above was placed in a pot with a diameter of 20 cm and a depth of 30 cm, and after planting corn (Maize, Da Feng 30), 28 to 33 ° C (low ), and cultured at 20-25 °C (night). Efficacy evaluation (evaluation of biomass and fertilizer effect enhancement) of the soil preparation composition by the soil mixing method using 12 pots containing mixed soil of the same composition (per set) was repeated for a total of 3 sets.

The amount of biomass of corn was measured by dry weight (g) after harvesting each stem and root of corn 60 days after sowing corn seeds, drying them at 70° C. for 72 hours, and the results are shown in Table 48 below.

treatment no. Factor biomass(g) N (Urea kg/ha) N (soil preparation composition (kg/ha)) One 1 (0) 1 (0) 85.33 2 1 (0) 2 (f-1 (300)) 168.33 3 1 (0) 3 (f-3 (600)) 232.00 4 1 (0) 4 (f-4 (900)) 249.33 5 2 (20) 1 (0) 140.67 6 2 (20) 2 (f-1 (300)) 221.67 7 2 (20) 3 (f-3 (600)) 261.00 8 2 (20) 4 (f-4 (900)) 278.33 9 3 (40) 1 (0) 200.50 10 3 (40) 2 (f-1 (300)) 259.00 11 3 (40) 3 (f-3 (600)) 281.33 12 3 (40) 4 (f-4 (900)) 303.00 13 4 (60) 1 (0) 213.67 14 4 (60) 2 (f-1 (300)) 267.50 15 4 (60) 3 (f-3 (600)) 287.67 16 4 (60) 4 (f-4 (900)) 349.33

The evaluation of the enhancement of the fertilizer effect according to the mixing of the soil preparation composition and the fertilizer is calculated by the following Equations 6 and 7, and Table 49 (Z value calculation result) and Table 50 ((ab) _ij value (interaction effect) calculation result ) is shown.

[Equation 6]

) (i=1,2,3,4; j=1,2,3,4) Z _ij =(y _ij -

) (i=1,2,3,4; j=1,2,3,4)

) 사이의 관계이며 상호작용 효과 계산에 사용되었다. 상기 수학식 6에서 y_ij는 하나의 실험세트에서 12개의 동일 실험군(treatment no.)의 바이오매스 평균 값을 나타내고,

는 3회 반복된 전체 실험세트(총 3세트)에서 총 36개의 동일 실험군(treatment no.)의 바이오매스 전체 평균값을 나타내며, i는 우레아 처리 농도에 따른 인덱스(표 48)이고 j는 토양조리제 조성물 처리 농도에 따른 인덱스(표 48)이다. The Z value is the average biomass value (y ij ) in one experimental set and the total biomass average value ( y _ij ) in the entire experimental set.

) and was used to calculate the interaction effect. In Equation 6, y _ij represents the average biomass value of 12 identical experimental groups (treatment no.) in one experimental set,

represents the overall average value of biomass of a total of 36 identical experimental groups (treatment no.) in the entire experimental set (3 sets in total) repeated three times, i is the index according to the urea treatment concentration (Table 48), and j is the soil preparation agent. Index according to composition treatment concentration (Table 48).

[Equation 7]

(ab) _ij = Z _ij -a _i -b _j (i=1,2,3,4; j=1,2,3,4)

a _i =∑Z _ij /4

b _j =∑Z _ij /4

In Equation 7, a _i represents the main effect on the biomass increase of the experimental group using only urea, and b _j is the calculation of the main effect on the biomass increase of the experimental group using only the soil preparation composition. (ab) _ij shows the interaction effect on biomass increase when urea and soil preparation composition are mixed at a specific concentration. (ab) If _ij is not 0, there is an interaction effect between urea and the soil preparation composition, and (ab) as the value of _ij increases, the interaction between urea and the soil preparation composition is excellent.

treatment no. y _ij Z _ij One 94 8.67 2 167 -1.33 3 239 7 4 255 5.67 5 125 -15.67 6 225 3.33 7 252 -9 8 275 -3.33 9 215 14.5 10 251 -8 11 284 2.67 12 311 8 13 177 -36.67 14 249 -18.5 15 332 44.33 16 382 32.67

treatment no. (ab) _ij value 6 17.96 7 18.36 8 18.63 10 21.03 11 26.3 12 30.63 14 11.8 15 21.96 16 52.3

As shown in Table 50, when the nitrogen source fertilizer (urea) and the soil preparation composition according to an example were mixed and used, (ab) the enhancement effect of the fertilizer effect was shown in terms of the _ij value (interaction effect), and the treatment As the concentration of the soil preparation used increased, the enhancement activity of the fertilizer effect increased.

Example 8. Synergistic effect when mixing soil preparation composition and fertilizer

As described in Example 6-1 above, the soil preparation composition was mixed with the soil (f-2 (450 kg/ha), f-3 (600 kg/ha), and f-4 (900 kg/ha)) and commercial 200 kg/ha N(Urea), 112 kg/ha P(P ₂ O ₅ ), 86 kg/ha K(K ₂ O), 15 kg/ha Zn, and 1 kg/ha B concentrations were mixed into the soil as a fertilizer available as a fertilizer. The soil preparation composition was mixed with dry soil for each concentration and sprayed on 3 plots of an area of 20 m ² of soil, and then, after mixing the composition with the soil on the soil surface with a tractor, corn (maize, Da Feng 30) seeds were sown. As dry soil, soil c (78.3% soil with an average particle size of 0.02 mm or larger, 14.9% soil with an average particle size of 0.02-0.002 mm, and 6.8% soil with an average particle size of 0.002 mm or less) was used as dry soil. Plants were cultivated in dry climate field conditions with an average annual temperature of 8.3°C for 50 years and an average annual precipitation of 365.7 mm for 50 years. The experiment was conducted in the same field as in Example 6-4, and the monthly rainfall and temperature of the area where the field experiment was performed are shown in FIG. 2 .

After 60 days of sowing corn seeds, the dry weight of the corn biomass was measured, and the moisture content of the soil 0 to 20 cm below the ground was measured and shown in Table 51.

As a control, soil F without fertilizer and soil preparation and commercially available 200 kg/ha N(Urea), 112 kg/ha P(P ₂ O ₅ ), 86 kg/ha K(K ₂ O), 15 kg/ha F-1 treated with Zn, 1 kg/ha B was used. In addition, commercially available soil preparation products (HumiCtech, Beijing Goldenway Bio-tech, Co., Ltd.) were mixed with soil at a concentration of 1200 kg / ha, and 200 kg / ha N (Urea) as commercially available fertilizer, 112 kg/ha P(P ₂ O ₅ ), 86 kg/ha K(K ₂ O), 15 kg/ha Zn, and 1 kg/ha B were mixed in soil at a concentration and used as a control (control group F-2).

Table 51 shows the total biomass (dry weight), chlorophyll content, and soil moisture content for the soil in which the soil preparation composition or control is mixed according to an example for corn cultured in the soil in which the soil preparation composition and the control are mixed The analysis results are shown.

Index Total biomass (g/plant) Chlorophyll content
(SPAD unit) Soil moisture content of 0-20 cm below the surface (wt%) F 124.7 26.9 16.85 f-2 619.3 52.3 23.47 f-3 650.9 53.3 26.01 f-4 691.3 54.8 25.37 F-2 481.2 50.9 20.93 F-1 582.7 41.1 17.21

As shown in Table 51, when the soil preparation composition according to an example is mixed with the soil containing the fertilizer, the total biomass, chlorophyll content, and soil moisture content than when the fertilizer is used alone (F-1) was significantly increased, and biomass, chlorophyll content, and soil moisture content were increased compared to using fertilizer and commercial soil preparation composition (F-2). It can be mixed with soil containing fertilizer to have a synergistic effect on plant growth and biomass increase.

For the analysis of fertilizer component runoff, 200 kg of dry soil and commercially available fertilizer component urea fertilizer (Urea, granule, Nitrogen content 46%) were applied to a 1000 ml measuring cylinder with 5 1 cm holes in the bottom using c soil. After mixing at a concentration of /ha, similarly to Example 6-1, the soil preparation composition was added at each concentration (f-2 (450 kg/ha), f-3 (600 kg/ha), f-4 (900 kg). /ha), and f-4 (1800kg/ha)) soil and mixed soil uniformly mixed with a 1000ml measuring cylinder, fill up to 25cm, give 250ml of water and leave for 1 day to dissolve the fertilizer component and absorb it into the soil made to be Then, 20ml of water was given, and the leaked water was collected through the hole drilled in the bottom of the measuring cylinder, and the amount of outflow and the amount of nitrogen fertilizer outflow were calculated.

Table 52 shows the results of performing fertilizer runoff analysis in the soil treated with the soil preparation composition according to an example.

Index N content
(mg N/kg soil) Volume of spilled water (ml) when 20ml water is added N runoff
(N leakage; %) F-1 134.61 12.85 2.26% f-2 150 10 1.61% f-3 157.7 6.86 0.99% f-4 165.4 6.57 1.25% f-5 182.7 6.29 0.91%

As shown in Table 52, in the soil treated with the soil preparation composition and fertilizer according to an example, the runoff effect of the fertilizer was significantly lower than that of the control group treated with only the fertilizer. It turns out that it has a spill prevention effect.

Example 9. Soil improvement and biomass increase effect of soil preparation composition containing xanthan gum and/or trace elements

The soil preparation composition 3A (including xanthan gum and trace elements) obtained in Example 1-3 was added at a concentration of 450 kg/ha (h-1), 600 kg/ha (h-2), and 900 kg/ha (h-3). Mixed with the soil, the soil preparation composition 3B (including trace elements) was mixed with the soil at a concentration of 450 kg / ha (h-4), 600 kg / ha (h-5), and 900 kg / ha (h-6) ( Table 53). In addition, as commercially available fertilizers, 200 kg/ha N(Urea), 112 kg/ha P(P ₂ O ₅ ), and 86 kg/ha K(K ₂ O) concentrations were mixed into the soil. As the soil mixed with the soil preparation composition and fertilizer, soil c (78.3% of soil with an average particle size of 0.02 mm or more, 14.9% of soil with an average particle size of 0.02-0.002 mm and 6.8% of soil with an average particle size of less than 0.002 mm) was used. As the control F, soil not mixed with the fertilizer and soil preparation composition was used, and as the control F-1, soil treated with only fertilizer without adding the soil preparation composition was used. Control F-1 is a commercially available fertilizer, 200 kg/ha N(Urea), 112 kg/ha P(P ₂ O ₅ ), 86 kg/ha K(K ₂ O), 0.02 kg/ha Mo(Na ₂ MoO ₄ ). 2H ₂ O), 1.2 kg/ha B(H ₃ BO ₃ ), 10 kg/ha Mn(MnSO ₄ H ₂ O), 10 kg/ha Fe(FeSO ₄ 7H ₂ O), 7 kg/ha Cu(CuSO) ₄ ·5H ₂ O) and 10 kg/ha Zn (ZnSO ₄ ·7H ₂ O) were used.

Soil preparation composition Molar ratio (Lys:CA) Xanthan Gum Content
(wt%) mixed concentration with soil h-1 Composition 3A 1.5:1 0.977 450kg/ha h-2 600kg/ha h-3 900kg/ha h-4 Composition 3B - 450kg/ha h-5 600kg/ha h-6 900kg/ha Control F not added - - - Control F-1 Add Fertilizer instead of Cooking Agent - - -

Put 8 kg of mixed soil mixed with soil preparation composition and/or fertilizer in a pot with a diameter of 20 cm and a depth of 30 cm, and use 12 pots of the same composition to improve the soil (bulk density of the soil, 0 to 20 cm below the ground) of soil moisture content) and maize (Maize, Da Feng 30) biomass amounts were measured. The amount of biomass of corn is the biomass generated from 12 pots treated with the same concentration of the soil preparation composition after 60 days have elapsed after sowing the corn seeds and culturing them at 28~33℃ during the day and 20~25℃ at night. Each stem and root of the mass was harvested, dried at 70° C. for 72 hours, and measured by dry weight, and the results are shown in Table 54.

In order to examine the soil improvement effect of the soil preparation composition according to an example, after 60 days have elapsed after sowing corn seeds, the moisture content of the soil 0 to 20 cm below the surface is measured, and the soil bulk density is measured, and the results are shown. 54.

Index Soil bulk density total biomass
(g/plant) Soil moisture content 0-20 cm below the surface (wt%) F 1.54 128.5 15.85 h-1 1.46 690.5 18.96 h-2 1.42 696.7 26.50 h-3 1.40 702.4 26.83 h-4 1.50 623.8 20.03 h-5 1.48 680.4 21.03 h-6 1.46 681.3 21.52 F-1 1.52 583.6 17.30

As shown in Table 54, although the control F-1 contains more trace components that can be used by plants than in the case of h-1 to h-6, the soil preparation composition according to an example compared to the controls F and F-1 (h-1 ~ h-6), the soil bulk density was lowered, the soil moisture content was increased, and the biomass of the corn was increased as the mixing concentration with the soil increased. The effects of soil improvement and biomass enhancement of the cooking composition were confirmed.

Example 10. Soil Erosion Inhibitory Effect of Soil Preparation Composition

By adding water to the soil preparation composition 1A (lysine and citric acid = 1.5:1 molar ratio) obtained in Example 1-1, the solid content of lysine and citric acid in the composition was 5 wt% (composition d-1), 10 wt. % (composition d-2) was prepared. As control E, deionized water was used as it was. The mixed molar ratio of lysine and citric acid, the solid content consisting of lysine and citric acid, and the xanthan gum content in the d-1 to d-2 compositions prepared by adding water to the soil preparation composition 1A as described above are the same as in Table 15.

The soil erosion inhibitory effect was evaluated under the same field conditions as those of Example 6-4 (rainfall and temperature conditions shown in FIG. 2). In the field, it is composed of soil c in the field (78.3% of soil with an average particle size of 0.02 mm or more; 14.9% of soil with an average particle size of 0.02-0.002 mm; 6.8% of soil with an average particle size of 0.002 mm or less). The soil preparation compositions d-1 and d-2 were respectively sprayed at 3L/m ² . For six months from May to October, 2019, the soils sprayed with the soil preparation composition and the soil not sprayed were subjected to dry climate field conditions (FIG. 2) with an annual average temperature of 8.3°C for 50 years and an average annual precipitation of 365.7 mm for 50 years. The result of confirming the erosion state of the soil by exposure is shown in FIG. 3 . After the experiment, the results of soil erosion inhibition are shown in FIG. 3 . In FIG. 3, the area (rectangle) divided into 4 landmarks (poles) in the left photo is the part where the soil preparation composition d-1 is sprayed, and in the right photo in FIG. 3, the area (rectangle) divided by 4 landmarks is the soil preparation The portion to which the first composition d-2 was applied is shown. The yellow arrow in FIG. 3 indicates the height (step difference) at which the soil is eroded by wind or rainfall in the area where the soil preparation composition is applied and the area that is not sprayed.

As shown in FIG. 3 , in the soil that is not sprayed with a soil conditioner, the soil surface is severely eroded by rainfall and wind, whereas in the soil sprayed with the soil conditioner, the soil surface is fixed compared to the unsprayed soil. Erosion was suppressed.

Reference Example 1. Evaluation of stability of a composition comprising lysine and an organic acid

(Lysine and citric acid = 1:1 molar ratio composition)

79 g of DIW (distilled water) was added to 100 g of 54 wt% L-lysine free form aqueous solution, and the mixture was diluted with stirring at room temperature (25° C.) for 30 minutes. 70.97 g of citric acid (CA) was slowly added to the diluted lysine at room temperature (25° C.) and stirred for 1 hour, followed by stirring at 60° C. for 1 hour. Then, after the reaction mixture reached room temperature (25° C.), the reaction was terminated to obtain 249.93 g of a composition. The content of the solids in this composition is about 50 parts by weight based on 100 parts by weight of the composition, the mixing molar ratio of lysine and citric acid is 1:1, and the solvent is deionized water.

(Lysine and other organic acids = 1:1 molar ratio composition)

In the same manner as the above method, a composition was prepared by changing only the type of organic acid.

In the same manner as in Example 1, except that acetic acid, glutamic acid, glutaric acid, tartaric acid, aspartic acid, fumaric acid, glyoxylic acid, 4-ketopimelic acid, pyruvic acid, and 1,3-acetonedicarboxylic acid were used instead of citric acid, respectively. The composition was prepared by carrying out according to the same method.

(Evaluation of precipitation)

It was evaluated whether the prepared composition formed a precipitate. Specifically, each composition was applied to a thickness of about 50 μm using a bar coater on a 50 μm thick OPP film (Samyoung Chemical Industries). After the film coated with the composition was left for 14 days at room temperature (25° C.) and 60±10% relative humidity, the shape change was evaluated by checking the surface change of the composition present on the OPP film.

The composition containing lysine and citric acid did not form a precipitate, whereas the composition containing other organic acids and lysine formed a precipitate, so adhesion could not be evaluated.

When the composition was prepared by mixing lysine and various organic acids, it was confirmed that all compositions did not form a precipitate and did not exhibit adhesion.

Reference Example 2. Solubility evaluation according to the solvent of the composition

According to the manufacturing method described in Reference Example 1, a composition comprising lysine and citric acid was prepared. (Mole ratio of lysine and citric acid = 1:1, solid content 50 parts by weight) 25 g of the following additional solvent was added to 50 g of the prepared composition, respectively, and stirred for 1 hour. After stirring, the additional solvents of the composition are methanol, toluene, benzene, chloroform, methylene chloride, dichloromethane, tetrahydrofuran (THF), ethyl acetate, dimethylformamide (DMF), dimethyl sulfoxide (DMSO), n-hexane Solubility in solvents was evaluated. As a result of the evaluation, the composition prepared in Example 1 was dissolved when methanol, an alcohol, was used as an additional solvent, but was not dissolved and precipitated in the other additional organic solvents described above.

Reference Example 3. Analysis of shape, viscosity, and initial adhesive force of the composition according to the solid content

According to the manufacturing method described in Reference Example 1, a composition comprising lysine and citric acid was prepared. However, the solid content in the composition is 10 wt%, 20 wt%, 30 wt%, 40 wt%, 50 wt%, 60 wt%, 61 wt%, 62 wt%, 63 wt%, 64 wt%, 65 wt%, respectively %, 66% by weight, 67% by weight, 68% by weight, 69% by weight, 70% by weight, 71% by weight, 72% by weight, 75% by weight to prepare a composition. (The molar ratio of lysine and citric acid is 1:1) (Compositions 1-1 to 1-19) The solid content was adjusted to the content of water.

1) Stability evaluation

A specific method for evaluating the stability of each composition having various molar ratios is as follows. About 1 g of each composition was weighed in an aluminum dish having a diameter of 5 cm. Thereafter, it was observed whether precipitates were formed in the composition for 14 days at room temperature (25° C.) and relative humidity of 60±10%.

2) Viscosity evaluation

Viscosity was measured using a rotary viscometer (manufacturer: LAMYRHEOLOGY, trade name: RM200 TOUCH CP400 or RM200 TOUCH) at 25±1° C., LV-1 type spindle, and the viscosity was measured at 60 rpm.

3) Initial adhesion evaluation

In the stability evaluation, the initial adhesive strength was evaluated for the composition in which no precipitation was formed. The initial adhesive strength was measured using a rheometer measuring equipment of Anton Paar, and the initial adhesive strength of the composition was compared through this equipment. Quantitative measurement of initial adhesive force made instantaneously by measuring the force generated by peeling the probe at the same speed after maintaining a 0.01 mm gap by contacting a SUS material probe having a diameter of 25 mm to the composition for 1 minute evaluation was made.

The evaluation results are shown in Table 55 below.

No. Lysine: CA
(mol ratio) solid content
(wt%) viscosity
(mPa.s) initial adhesion
(mJ) Whether a precipitate is formed 1-1 1:1 10 10.08 0.21 radish 1-2 1:1 20 11.84 0.217 radish 1-3 1:1 30 13.54 0.216 radish 1-4 1:1 40 16.24 0.22 radish 1-5 1:1 50 26.68 0.222 radish 1-6 1:1 60 85.28 0.523 radish 1-7 1:1 61 91.45 0.562 radish 1-8 1:1 62 99.13 0.614 radish 1-9 1:1 63 115.22 0.652 radish 1-10 1:1 64 125.35 0.751 radish 1-11 1:1 65 168.5 0.783 radish 1-12 1:1 66 184.15 0.899 radish 1-13 1:1 67 223.56 0.921 radish 1-14 1:1 68 290.52 1.12 radish 1-15 1:1 69 424.2 1.24 radish 1-16 1:1 70 657.67 1.48 radish 1-17 1:1 71 Presence of insoluble substances (CA) 1-18 1:1 72 Presence of insoluble substances (CA) 1-19 1:1 75 Presence of insoluble substances (CA)

Referring to Table 55, when the solid content in the composition is 71 wt% or more, a precipitate is formed, whereas when the solid content in the composition is 10 wt% to 70 wt%, no precipitate is formed and the liquid phase is maintained.

Reference Example 4. Comparison of initial adhesive strength

The adhesive strength of the conventional adhesive and the composition of the present application was compared.

According to the manufacturing method described in Reference Example 1, a composition comprising lysine and citric acid was prepared. However, the content of water was adjusted so that the content of solids in the composition was 10% by weight. (Mole ratio of lysine and citric acid = 1:1) (Composition 2-1)

After preparing a commercially available polyvinyl alcohol based adhesive (PVA 088-50, Qingdao Sanhuan Colorchem CO., LTD), the content of water was adjusted so that the solid content was 10% by weight to the composition (hereinafter, the control 1) was prepared.

Using the same method as described in Reference Example 3, the viscosity and initial adhesive strength of the composition of the present application (solid content 10% by weight) and the Control 1 were evaluated. The evaluation results are shown in Table 56 below.

No. solid content
(wt%) viscosity
(mPa.s) initial adhesion
(mJ) 2-1 10 10.1 0.21 Control 1 (PVA system) 10 43.49 0.201

Referring to Table 56, the composition according to the present application exhibited an equivalent level of initial adhesive strength as compared to the polyvinyl alcohol-based adhesive composition (control group 1).

Reference Example 5. Evaluation of peel strength according to solvent

According to the manufacturing method described in Reference Example 1, a composition containing lysine and citric acid was prepared (below 3-1 to 3-3). However, the molar ratio of lysine and citric acid was 1.5:1, 1:1, and 1:1.5, respectively, to prepare a composition (solid content 50 parts by weight).

According to the preparation method described in Reference Example 1, a composition containing lysine and citric acid was prepared (below 3-4 to 3-6). However, the molar ratio of lysine and citric acid was 1.5:1, 1:1, and 1:1.5, respectively, to prepare a composition, and methanol was added as a solvent. The weight ratio of the deionized water and methanol was 1:1. (50 parts by weight of solid content)

According to the manufacturing method described in Reference Example 1, a composition containing lysine and citric acid was prepared (below 3-7 to 3-8). However, methanol was added in addition to deionized water, and the weight ratio of deionized water and methanol was set to 6:4 and 4:6. (Mole ratio of lysine and citric acid = 1:1, solid content 50 parts by weight)

As a control, a commercially available acrylic adhesive (K901, Hansung P&I) (hereinafter, Control 2) (solid content 59% by weight) was prepared. For each of the compositions, peel strength was evaluated according to the following method, and the evaluation results are shown in Table 57 below.

1) Peel strength

After preparing the PET film (film size: 120mm*25mm, thickness: 38㎛, or 50㎛) using a bar coater, the sample was coated on the surface of the PET film (50㎛ thickness) to a thickness of 11㎛. After drying in an oven at 60℃ for 4 minutes, lamination was performed with PET film (38㎛ thickness) using dry laminatior equipment (roller speed 1.9m/min, roller temp. 60℃). After the lamination was completed, the specimen was dried in an oven controlled at 30° C. for 72 hours. The peel strength of the dried specimen was measured according to ASTM D1876 “Measurement of 180° T Peel Strength”. The evaluation results are shown in Table 57 below.

No. Lysine: CA
(mol ratio) menstruum solid content
(wt%) peel strength
(N/25mm) 3-1 1.5:1 DIW 50 5.91 3-2 1:1 5.45 3-3 1:1.5 4.92 3-4 1.5:1 DIW and methanol (1:1 wt ratio) 7.11 3-5 1:1 6.92 3-6 1:1.5 6.18 3-7 1:1 DIW and methanol (6:4 wt ratio) 6.28 3-8 1:1 DIW and methanol (4:6 wt ratio) - Control 2
(Acrylic) - 59 6.61

Referring to Table 57, the PET film adhered to the stainless steel by the composition according to the present application was separated within 1 hour, and provided a similar peel strength even with a lower solid content compared to Control 2.

In addition, when water and alcohol were used as solvents, the peel strength was further improved. It is judged that the improved peel strength is because the composition using the mixed solvent has a lower contact angle compared to the composition containing only deionized water, and as a result, the coating property to the substrate is better. However, when the content of deionized water and alcohol was in a weight ratio of 4:6, phase separation occurred in the composition, so that it could not be used.

Reference Example 6. Analysis of the composition in the composition according to the reaction temperature condition

In preparing the composition according to an example, the composition in the composition according to temperature conditions was analyzed.

1) Preparation at 0° C. (low temperature): 79 g of DIW (distilled water) was added to 100 g of 54 wt% lysine aqueous solution and stirred at 0° C. (T1) for 30 minutes. To the diluted result, 70.97 g of citric acid was slowly added at 0° C. (T2) and stirred for 1.5 hours to prepare a composition. (Solid content: 50% by weight, mixing molar ratio of lysine and citric acid = 1:1) An ice bath was used to maintain the same temperature while stirring.

2) Preparation at 25° C. (room temperature): 79 g of DIW (distilled water) was added to 100 g of 54 wt% lysine aqueous solution and stirred at room temperature 25° C. (T1) for 30 minutes. To the diluted result, 70.97 g of citric acid was slowly added at 25° C. (T2) and stirred for 1.5 hours to prepare a composition. (solid content: 50 wt %, molar ratio of lysine and citric acid = 1:1). A temperature controller was used to maintain the same temperature while the composition was stirred (hereinafter the same).

3) Preparation at 60°C: A composition was prepared in the same manner as in 2) above, except that T2 was changed to 60°C.

4) Preparation at 80°C: A composition was prepared in the same manner as in 2) above, except that T2 was changed to 80°C.

5) Preparation at 240°C: A composition was prepared in the same manner as in 2) above, except that T2 was changed to 240°C.

As a result of preparing the composition according to the above method, carbides were formed at 240° C. and the composition could not be prepared. Therefore, component analysis was performed using ¹ H NMR for the compositions prepared at 0 ℃, 25 ℃, 60 ℃ and 80 ℃.

The NMR analyzer and conditions used in the present application are as follows.

Superconducting Fourier Transform Nuclear Magnetic Resonance Spectrometer (400MHz) Model Name: AVANCE II 400, Manufacturer: Bruker Biospin (Maget field strength 9.4 Tesla, Field driftrate: 4Hz/hr, Obervable Freguency: 400Mhz 1H, Sensitivity: 220:1(1H), Variable Temp.: -70~+110℃), solvent: D ₂ O

¹ H NMR analysis was performed on the compositions prepared at 0° C. (Sample 1), 25° C. (Sample 2), and 80° C. (Sample 3). The results of NMR analysis are shown in FIG. 1 . Referring to Figure 1, the compositions prepared at 0 ℃, 25 ℃, and 80 ℃ all of the ¹ H NMR peak appeared at the same position, chemical shift (chemical shift) did not occur, they all have the same composition It can be seen that there is That is, in the composition prepared at 0 ° C, 25 ° C, and 80 ° C, lysine and citric acid are present in a mixture state and there is no formation of a condensate of lysine and citric acid, or even if a condensate is formed, it is contained in a very small amount as an impurity. Able to know.

Claims (20)

A composition for soil improvement for maintaining or increasing the moisture content of the soil comprising lysine and citric acid in a molar ratio of 5:1 to 3:1, at least one selected from the group consisting of plants, plant seeds, soil, and plant planting soil In a method for enhancing biomass comprising the step of treating
The solid content in the composition for soil improvement comprises 0.1 to 30 parts by weight based on 100 parts by weight of the composition for soil improvement,
The composition for soil improvement contains 5 to 20 parts by weight of the trace element based on 100 parts by weight of the solid content,
The composition for soil improvement comprises 0.01 to 5 parts by weight of a thickener based on 100 parts by weight of the composition for soil improvement,
The soil includes 30 to 80 parts by weight of soil having a particle size exceeding 0.02 mm; 10 to 40 parts by weight of soil having a particle size of 0.002 mm or more to 0.02 mm or less; and 5 to 40 parts by weight of soil having a particle size of less than 0.002 mm,
A method of enhancing biomass. The method of claim 1, wherein the step of treating the composition for soil improvement on one or more selected from the group consisting of plants, plant seeds, soil, and plant planting soil comprises immersion method, soil mixing method, soil spraying method, application Treatment, fumigation treatment, spraying, irrigation, and indirect absorption method will be carried out by at least one method selected from the group consisting of, the biomass enhancement method. The method of claim 2, wherein the soil mixing method is mixed with the soil by adding the composition for soil improvement at a concentration of 100 to 4500 kg/ha. According to claim 1, wherein the plant is poplar (Poplar), Caragana (Caragana), corn (maize), ryegrass (ryegrass), rice, rye, wheat, barley, hops, soybean, potato, red bean, oat, sorghum, Cho, Arabidopsis, Chinese cabbage, radish, red pepper, strawberry, tomato, watermelon, cucumber, cabbage, melon, pumpkin, green onion, onion, carrot, ginseng, tobacco, cotton, sesame, sugar cane, sugar beet, perilla, peanut, rapeseed , apple tree, pear tree, jujube tree, peach, poppy, grape, tangerine, persimmon, plum, apricot, banana, rose, gladiolus, gerbera, carnation, chrysanthemum, lily, tulip, red clover, orchard grass, alfalfa, tall fescue, And at least one selected from the group consisting of perennial ryegrass, biomass enhancement method. delete delete delete In a soil improvement method comprising the step of treating the soil with a composition for soil improvement for maintaining or increasing the moisture content of the soil comprising lysine and citric acid in a molar ratio of 5:1 to 3:1,
The solid content in the composition for soil improvement comprises 0.1 to 30 parts by weight based on 100 parts by weight of the composition for soil improvement,
The composition for soil improvement contains 5 to 20 parts by weight of the trace element based on 100 parts by weight of the solid content,
The composition for soil improvement comprises 0.01 to 5 parts by weight of a thickener based on 100 parts by weight of the composition for soil improvement,
The soil includes 30 to 80 parts by weight of soil having a particle size exceeding 0.02 mm; 10 to 40 parts by weight of soil having a particle size of 0.002 mm or more to 0.02 mm or less; and 5 to 40 parts by weight of soil having a particle size of less than 0.002 mm,
How to improve the soil. The method of claim 8, wherein the improvement of the soil is at least one selected from the group consisting of the following (1) to (5):
(1) decrease the bulk density of the soil;
(2) maintaining the bulk density of the soil;
(3) prevent erosion of the soil;
(4) prevention of spillage of fertilizer components in the soil; and
(5) Maintaining soil acidity in the range of greater than pH4.5 to less than pH8.0. The method of claim 8, wherein the step of treating the soil with the composition for soil improvement is selected from the group consisting of immersion method, soil mixing method, soil spraying method, application treatment, fumigation treatment, spraying, irrigation, and indirect absorption method. A method for improving the soil, which is carried out in more than one way. The method of claim 8, wherein the soil further comprises a fertilizer component. delete The method of claim 9, wherein the maintenance of the moisture content of the soil is the rate of change of the moisture content of the soil after 20 to 200 days under the conditions of a daytime temperature of 25 to 35 ℃, a night temperature of 15 to 25 ℃, and a relative humidity of 35 to 50%RH. This is 0.1 to 80%, the soil improvement method. delete delete In the composition for soil improvement for maintaining or increasing the moisture content of the soil comprising lysine and citric acid in a molar ratio of 5:1 to 3:1,
The solid content in the composition for soil improvement comprises 0.1 to 30 parts by weight based on 100 parts by weight of the composition for soil improvement,
The composition for soil improvement contains 5 to 20 parts by weight of the trace element based on 100 parts by weight of the solid content,
The composition for soil improvement comprises 0.01 to 5 parts by weight of a thickener based on 100 parts by weight of the composition for soil improvement,
The soil includes 30 to 80 parts by weight of soil having a particle size exceeding 0.02 mm; 10 to 40 parts by weight of soil having a particle size of 0.002 mm or more to 0.02 mm or less; and 5 to 40 parts by weight of soil having a particle size of less than 0.002 mm,
A composition for improving soil. The composition of claim 16, further comprising a fertilizer component. delete delete A fertilizer composition comprising the composition for soil improvement of claim 16 .

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