KR102431352B1 - Collagen composition with inner beauty effect - Google Patents

Abstract

The present invention relates to a collagen composition with effects for inner beauty, and specifically, can provide a collagen-containing tea composition, a collagen powder and a collagen jelly, in which raw materials such as extracts of bellflower, pear, pomegranate and Perilla frutescens leaves are mixed in a specific ratio, and fish collagen is contained together therewith, thereby alleviating or reducing skin wrinkles, whitening skin, maintaining skin oil-moisture balance, and moisturizing skin. In addition, the present invention overcomes the limitations of existing skin external agents that act locally only on an application area, and acts on the entire epidermal layer and the dermal layer skin tissues to provide a product that actually exhibits skin functional efficacy.

Description

Collagen composition with inner beauty effect

The present invention relates to a collagen composition having an inner beauty effect, and more specifically, it contains a complex of natural material extracts to improve or alleviate skin wrinkles, skin whitening, maintain skin oil-water balance and skin moisturizing effect. to the collagen composition.

In modern society, the health functional food market is growing as well-being lifestyle and self-medication trends are spreading along with the trend of aging, and as interest in natural and healthy skin is growing, interest in inner beauty related foods is also increasing. have.

There are 4 types of health functional food ingredients related to skin health functionalities as notified raw materials and 15 types of individually recognized ingredients. 5th highest number of acknowledgments.

In general, as a method to prevent skin damage, functional cosmetics containing UV blockers or substances effective for wrinkle improvement are used, or drugs are applied directly to the skin. However, these external preparations for skin have a limitation in that their effect is temporary and only gives a local effect to the application site. On the other hand, inner beauty materials containing functional substances are not used directly on the skin but are ingested through the digestive system to create a healthy skin environment in the body, acting on the entire epidermis and dermis skin tissue to show wrinkle improvement, whitening and moisturizing effects. have.

Inner beauty materials are mostly developed or produced using the results of research and raw materials produced in foreign countries. Therefore, for the growth of the domestic inner beauty market, it is necessary to directly develop the production technology for inner beauty food materials and to actively develop technology in the skin research field. In addition, it is necessary to actually develop a product with inner beauty efficacy through application and verification as a composition, going beyond simply developing raw materials.

Republic of Korea Patent No. 10-2271861 (registration date: June 26, 2021) relates to a food composition for skin improvement containing a natural ingredient extract, and includes a bellflower extract, a Chinese cabbage extract, a pomegranate extract and a perilla leaf extract to wrinkle the skin A food composition that improves or relieves, whitens skin, maintains skin oil-water balance, and moisturizes skin is described. Republic of Korea Patent No. 10-2169920 (registration date: October 20, 2020) relates to a composition for preventing or treating respiratory tract inflammatory diseases comprising a Schisandra extract, a pear extract and a bellflower extract, and a Schisandra extract and pear mixed in a specific ratio. It has been described for a composition for preventing or treating respiratory inflammatory diseases, including the extract and bellflower extract, which inhibits the expression level of inflammatory cytokines.

The present invention mixes natural materials such as bellflower extract, pear extract, pomegranate extract, and perilla leaf extract in a specific ratio and contains fish collagen to improve or alleviate skin wrinkles, skin whitening, maintain skin oil-water balance, and moisturize the skin An object of the present invention is to provide a collagen composition.

The present invention provides a collagen-containing tea composition prepared by adding bellflower extract, pear extract, pomegranate extract and perilla leaf extract to fish collagen.

In addition, it provides a collagen powder in which bellflower extract, pear extract, pomegranate extract and perilla leaf extract are added to fish collagen powder.

In addition, in the jelly containing fish collagen, bellflower extract, pear extract, pomegranate extract and perilla leaf extract are added.

The present invention mixes natural ingredients such as bellflower extract, pear extract, pomegranate extract, and perilla leaf extract in a specific ratio, and contains fish collagen to improve or alleviate skin wrinkles, skin whitening, maintain skin oil-water balance, and moisturize skin can provide a collagen-containing tea composition, collagen powder and collagen jelly. In addition, the present invention overcomes the limitation that the existing external skin preparations only act locally on the application site, and can provide a product in which skin functional efficacy is actually exhibited by acting on the entire epidermal layer and the dermis layer skin tissue.

1 is a graph showing the cytotoxicity of the three products of the present invention to B16-F10 cells (A), HS68 cells (B), and HaCaT cells (C).
2 is a graph showing the melanin production inhibitory effect of the three products of the present invention.
3 is a graph showing the procollagen production effect of the three products of the present invention.
4 is a graph showing the hyaluronic acid production effect of the three products of the present invention.
5 is a graph showing the ceramide generation effect of the three products of the present invention.

The present invention is to provide an inner beauty product that overcomes the limitation in which the existing external skin preparations act only locally on the application site, and that acts on the entire epidermal layer and the dermis layer skin tissue to actually exhibit skin functional efficacy.

The present inventors have previously reported that using bellflower extract, pear extract, pomegranate extract and perilla leaf extract in Patent No. 10-2271861 (registration date: June 26, 2021) to exhibit skin wrinkle improvement effect, skin whitening effect, and skin moisturizing effect. have confirmed that In addition, by treating the complex of each extract in a ratio of various mixing ratios, it has been confirmed that their efficacy is exhibited. However, it is necessary to check whether the efficacy is exhibited when actually commercializing natural materials because it is not guaranteed that the functionalities are exhibited on the skin when commercialized. For this purpose, as a result of testing products prepared in various mixing ratios, it was confirmed that skin functionality was exhibited in products having an optimal mixing ratio (1:1:1:1).

Specifically, the present invention provides a collagen-containing tea composition prepared by adding bellflower extract, pear extract, pomegranate extract and perilla leaf extract to fish collagen. In addition, it provides a collagen powder in which bellflower extract, pear extract, pomegranate extract and perilla leaf extract are added to fish collagen powder. In addition, in the jelly containing fish collagen, bellflower extract, pear extract, pomegranate extract and perilla leaf extract are added.

Bellflower is a perennial plant of the bellflower family, and is also called plantain, gilgyeong, gilgyeongchae, dorat, and baekyak. Bellflower roots are used for food and are known to have anti-anemia, tonsillitis, and anti-cancer effects.

Pears are the fruits of the pear tree belonging to the Rosaceae family. The skin is yellow and charred brown, and the flesh has a crunchy texture. Pears are used in a variety of ways, such as fresh fruits, beverages, and alcoholic beverages. Pears are known to promote diuresis, prevent constipation, and prevent respiratory diseases.

A pomegranate has a round, hard, yellowish exterior that surrounds the flesh, which contains many seeds. Pomegranate is consumed by eating the flesh or in the form of beverages such as fruit juice. Both the fruit and the peel are known to be effective in preventing hypertension and arteriosclerosis.

A perilla leaf refers to the leaf or tip of a leaflet. Leaflets are annual plants of the Lamiaceae family, also called perilla and chazugi. It is known to be effective in relieving digestive disorders and improving cough or asthma.

In the present invention, bellflower extract, pear extract, pomegranate extract, and perilla leaf extract can be prepared according to a conventional method known in the art, that is, under ordinary temperature and pressure conditions, using a conventional solvent. It is better to use purified water. In addition, in the present invention, each extract was extracted using the optimal extraction conditions that can be extracted with maximum efficiency. Preferably, the bellflower extract of the present invention is extracted under the conditions of 51-55 ℃, 25-29 h, the pear extract is preferably extracted under the conditions of 94-98 ℃, 18-22 h, and the pomegranate extract is 94-98 ℃, It is recommended to extract under the conditions of 12~16h, and extract the perilla leaf extract under the conditions of 51~55℃ and 25~29h. More preferably, each extract is concentrated under reduced pressure and then freeze-dried and powdered for use.

On the other hand, according to the following experiment, the three products (collagen-containing tea composition, collagen powder, collagen jelly) of the present invention did not show cytotoxicity at concentrations of 0.1, 0.25, 0.5 and 1 mg/ml, melanin production inhibitory ability, pro It was confirmed that the collagen production ability, hyaluronic acid and ceramide production ability were excellent. Through this, it was confirmed that the three types of products of the present invention were excellent in skin whitening, skin wrinkle improvement, skin oil-water balance maintenance, and skin moisturizing function.

On the other hand, in the collagen composition of the present invention (collagen-containing tea composition, collagen powder, collagen jelly), it is preferable to contain bellflower extract, pear extract, pomegranate extract, and perilla leaf extract in a weight ratio of 1:1:1:1. good night. Through this, skin functions (whitening, wrinkle improvement, skin oil-water balance maintenance and skin moisturizing) can be excellently exhibited.

On the other hand, in the collagen-containing tea composition of the present invention, when preparing the tea composition, any known in the art may be additionally added to be prepared, but preferably hyaluronic acid, synthetic flavor (pomegranate flavor), red beet It is good to contain any one or more selected from concentrate, elastin hydrolyzate, pomegranate concentrate, DL-malic acid, vitamin C, xylitol, complex golden extract, and fructooligosaccharide together.

On the other hand, the collagen-containing tea composition of the present invention can be mixed with drinking water such as hot or cold water to drink a tea composition of various concentrations, and individual consumers can drink it by adjusting the appropriate concentration.

On the other hand, in the collagen powder of the present invention, any one known in the art can be additionally added to prepare the powder composition, but preferably hyaluronic acid, synthetic flavor (pomegranate flavor), red beet concentrated powder , elastin hydrolyzate, DL-malic acid, vitamin C, xylitol, dextrin, milk cream powder, it is good to contain any one or more selected from glucose together.

Meanwhile, in the collagen jelly of the present invention, any one known in the art may be additionally added to prepare the jelly composition, but preferably hyaluronic acid, red beet concentrate, elastin hydrolyzate, pomegranate concentrate , it is good to contain any one or more selected from agar.

Hereinafter, the present invention will be described in more detail in the following Examples and Experimental Examples. However, the scope of the present invention is not limited only to the following examples and experimental examples, and includes all modifications of the technical idea equivalent thereto.

[Production example: bellflower extract, pear extract, pomegranate extract, perilla leaf extract preparation]

After peeling and washing, 100 g of dried bellflower was heated and extracted with purified water 10 times the weight of the dried bellflower, and extraction conditions were set to 53.7868 °C and 27.7279 h for a bellflower extract.

To 100 g of dried pears after washing without removing the peel, purified water 10 times the weight of the dried pears was added for extraction, and extraction conditions were set to 96.2132 °C and 20.0140 h to obtain a pear extract.

After clean washing, 100 g of dried pomegranate fruit (with skin removed, including seeds) was added with purified water 10 times the weight of the dried pomegranate and extracted at 96.2132 ° C. for 14.1000 h to obtain a pomegranate extract.

After washing, purified water 10 times the weight of the dried perilla leaves was added to 100 g of dried perilla leaf for extraction, and the extraction was performed at 53.7868 ° C. and 27.7279 h to obtain a perilla leaf extract. After extraction, each extract was concentrated under reduced pressure and lyophilized to solidify, and then the powder was used to prepare the following products.

[Example 1: Preparation of collagen-containing tea composition containing bellflower extract, pear extract, pomegranate extract, and perilla leaf extract]

In this example, a collagen-containing tea composition (referred to as 'Inner Beauty More Maekreum Collagen') containing bellflower powder, pear powder, pomegranate powder, and perilla leaf powder obtained in Preparation Example was prepared, and the detailed composition thereof is shown in Table 1 below. was like

Raw material name Mixing ratio (based on 100g) bellflower powder 1.75 pear powder 1.75 pomegranate powder 1.75 perilla powder 1.75 fish collagen 0.6 hyaluronic acid 0.12 Synthetic Flavor (Pomegranate Flavor) 0.1 Red Beet Concentrate 3.0 Elastin hydrolyzate 0.02 Pomegranate Concentrate 10.0 DL-Malic Acid 0.1 vitamin C 0.1 xylitol 1.0 Complex Golden Extract 3.0 fructooligosaccharide 8.0 Purified water 66.96 Sum 100

[Example 2: Preparation of collagen powder containing bellflower extract, pear extract, pomegranate extract, and perilla leaf extract]

In this example, a collagen powder (referred to as 'Inner Beauty Moremulleum collagen powder') including bellflower powder, pear powder, pomegranate powder, and perilla leaf powder obtained in Preparation Example was prepared, and the detailed composition thereof is shown in Table 2 below. It was like

Raw material name Mixing ratio (based on 100g) bellflower powder 1.0 pear powder 1.0 pomegranate powder 1.0 Perilla leaf powder 1.0 Fish Collagen Powder 55 hyaluronic acid 1.0 Synthetic Flavor (Pomegranate Flavor) 1.0 Red Beet Concentrate Powder 10 Elastin hydrolyzate 0.02 DL-Malic Acid 0.1 vitamin C 1.0 xylitol 5.0 dextrin 17.88 milk cream powder 2.0 glucose 3.0 Sum 100

[Example 3: Preparation of collagen jelly containing bellflower extract, pear extract, pomegranate extract, and perilla leaf extract]

In this example, collagen jellies (referred to as 'Inner Beauty More Marleum Collagen Stick') containing bellflower powder, pear powder, pomegranate powder, and perilla leaf powder obtained in Preparation Example were prepared, and the detailed composition thereof is shown in Table 3 below. It was like

Raw material name Mixing ratio (based on 100g) bellflower powder 1.0 pear powder 1.0 pomegranate powder 1.0 Perilla leaf powder 1.0 fish collagen 5.0 hyaluronic acid 1.0 Red Beet Concentrate 1.0 elastin hydrolyzate 0.02 Pomegranate Concentrate 87.18 agar 1.8 Sum 100

[Experimental Example 1: Measurement of cell viability of three products of the present invention]

In this experimental example, the cytotoxicity of the three products (Inner Beauty Clearer Collagen, Inner Beauty Clearer Collagen Powder, Inner Beauty Clearer Collagen Stick) developed in Examples 1 to 3 was evaluated.

Due to the toxicity of the sample on the cells during the cell experiment, it may affect the nucleus, mitochondria, cytosol, etc., resulting in inaccurate results during the experiment. to proceed, and the cell viability was measured. Cell viability tests were performed on B16-F10 (mouse-derived melanoma cells), HS68 (human-derived fibroblasts), and HaCaT (keratinocytes) cells. Each cell was cultured and seeded at 1x10 ⁴ cells in 96 wells and cultured for 24 hours. Thereafter, the three products were treated with different concentrations (0.1, 0.25, 0.5 and 1 mg/ml), and cultured for 24 hours. Thereafter, the medium was removed, washed with PBS, treated with MTT reagent (0.5 mg/ml), and cultured for 4 hours, washed with PBS and dried. After drying, 100 μl of DMSO was treated to elute formazan, and absorbance was measured at 570 nm.

As a result, as shown in FIG. 1A, when the three products of the present invention were treated in the concentration range (0.1, 0.25, 0.5 and 1 mg/ml), it appeared that the cell viability of B16-F10 was not affected. It was confirmed that no toxicity was observed. As shown in FIG. 1B, when the three products of the present invention were treated in the concentration range (0.1, 0.25, 0.5 and 1 mg/ml), it did not appear to affect the cell viability of HS68, indicating that cytotoxicity did not appear. Confirmed. As shown in FIG. 1C, when the three products of the present invention were treated in the concentration range (0.1, 0.25, 0.5 and 1 mg/ml), it did not appear to affect the cell viability of HaCaT, indicating that cytotoxicity did not appear. Confirmed.

[Experimental Example 2: Confirmation of melanin production inhibitory ability of the three products of the present invention]

As melanin increases abnormally due to ultraviolet (UV) light and external environment, blemishes or pigmentation occurs on the skin. Therefore, it can be confirmed that there is a whitening effect by suppressing it. In this experimental example, the melanin production inhibitory ability of the three products (Inner Beauty Clearer Collagen, Inner Beauty Clearer Collagen Powder, Inner Beauty Clearer Collagen Stick) developed in Examples 1 to 3 was confirmed. As a control, arbutin was used.

B16-F10 cells, which are mouse-derived melanoma cells, were aliquoted in a 12-well plate to 5x10 ⁴ cells/well and cultured for 24 hours. Thereafter, the cell culture medium was exchanged with a culture medium containing 100 nm α-melanocyte-stimulating hormone (α-MSH, Sigma-Adlrich) and three products of various concentrations (0.01, 0.1, 0.25, 0.5, 1.0 mg/ml). Cells were cultured. After culturing the cells for 72 hours, the cell monolayer was washed with PBS, then the cells were recovered, and centrifuged at 12,000 rpm for 10 minutes to obtain a pellet. 1N NaOH containing 10% dimethylsulfoxide (DMS0) was added to the obtained pellet, dissolved at 60°C, and absorbance was measured at a wavelength of 490 nm (For each group, n=3±SD, **p<0.005, ** *p<0.001 compared by control). The amount of melanin was calculated from a calibration curve using synthetic melanin, the protein was measured using the BCA protein assay kit (Thermo Scientific), the amount of melanin production was normalized to the amount of protein, and the value of the group treated only with α-MSH was set to 100. Values are expressed as mean ± SEM. # p<0.05, ## p<0.01, ### p<0.001 significantly different from that of α-MSH(-)/0 μg/mL group. * p<0.05, ** p<0.01, *** p<0.001 significantly different from that of α-MSH(+)/0 μg/mL group).

As a result, as shown in FIG. 2, the three products of the present invention inhibited melanin production induced by α-MSH in a concentration-dependent manner, and it was confirmed that there was a whitening function through this. was able to confirm that

[Experimental Example 3: Confirmation of procollagen production ability of three types of products of the present invention]

Although the dermal layer of the skin is organically composed of fibroblasts, collagen, and elastin, wrinkles occur as the organic structures such as collagen and elastin are destroyed by skin damage such as aging and ultraviolet rays. Procollagen is converted into collagen and increases the content of collagen in the dermal layer, helping to alleviate wrinkles. In this experimental example, the procollagen-producing ability of the three products (Inner Beauty Clearer Collagen, Inner Beauty Clearer Collagen Powder, Inner Beauty Clearer Collagen Stick) developed in Examples 1 to 3 was confirmed. As a control, 1 μM of retinol was used.

Human-derived fibroblasts, HS68 cells, were aliquoted in a 24-well plate to 1x10 ⁵ cells/well, and the cells were cultured for 24 hours. Thereafter, the three products were exchanged with a serum-free cell culture medium containing various concentrations (0.1, 0.25, 0.5, 1.0 mg/ml) and cultured for 24 hours. Then, the cell culture medium was collected and the collagen content was measured according to the method suggested by the manufacturer using the Procollagen Type I C-Peptide (PIP) ELA Kit (Takara), respectively (Values are expressed as mean ± SEM. * p<0.05) , **p<0.01, ***p<0.001 significantly different from that of 0 μg/mL group).

As a result, as shown in FIG. 3 , the three products of the present invention had an excellent effect of accelerating the production of procollagen in a concentration-dependent manner, and it was confirmed that there was a wrinkle-relieving function.

[Experimental Example 4: Confirmation of hyaluronic acid (HA) and ceramide production ability of three types of products of the present invention]

Keratinocytes maintain the oil-water balance of the skin through intercellular lipids and moisture content. If the amount of intracellular lipid decreases or moisture is insufficient, keratin occurs or problems such as skin inflammation occur, so proper oil and moisture control is required. In this experimental example, the ability to produce hyaluronic acid and ceramides of the three products (Inner Beauty Clearer Collagen, Inner Beauty Clearer Collagen Powder, Inner Beauty Clearer Collagen Stick) developed in Examples 1 to 3 was confirmed. As a control, 1 μM of GlcNAc (N-Acetylglucosamine) was used.

First, in order to measure the hyaluronic acid produced by HaCaT cells, which are human-derived keratinocytes, cells were plated at 1x10 ⁴ cells/well in a 48-well plate and cultured for 24 hours. Thereafter, the three products were exchanged with a serum-free cell culture medium containing various concentrations (0.1, 0.25, 0.5, 1.0 mg/ml) and cultured for 24 hours. Thereafter, the cell culture medium was collected, centrifuged at 5,000 rpm for 10 minutes, and the supernatant was taken and stored at -70°C. The hyaluronic acid content in the cell culture was measured according to the method suggested by the manufacturer using an ELISA kit (R&D systems) (Values are expressed as mean ± SEM. * p<0.05, ** p<0.01, *** p <0.001 significantly different from that of 0 μg/mL group).

As a result, as shown in FIG. 4 , it was confirmed that the three products of the present invention had an excellent effect of generating hyaluronic acid in a concentration-dependent manner, thereby maintaining oil-water balance and moisturizing function.

Next, in order to measure the ceramide produced by HaCaT cells, which are human-derived keratinocytes, the cells were seeded in a 48-well plate at 2.5x10 ⁴ cells/well and cultured for 24 hours. Thereafter, the cells were cultured for 24 hours by exchanging them with a serum-free cell culture medium containing the three products at various concentrations (0.1, 0.25, 0.5, 1.0 mg/ml). Thereafter, the cell culture medium was collected, centrifuged at 5,000 rpm for 10 minutes, and the supernatant was taken and stored at -70°C. The ceramide content in the cell culture was measured according to the method suggested by the manufacturer using an ELISA kit (R&D systems) (Values are expressed as mean ± SEM. * p<0.05, ** p<0.01, *** p< 0.001 significantly different from that of 0 μg/mL group).

As a result, as shown in FIG. 5 , it was confirmed that the three products of the present invention had an excellent effect of generating ceramide in a concentration-dependent manner, thereby maintaining oil-water balance and moisturizing function.

Claims (3)

It was prepared by adding bellflower extract, pear extract, pomegranate extract and perilla leaf extract to fish collagen in a weight ratio of 1:1:1:1,
A collagen-containing tea composition comprising 0.6% by weight of fish collagen, 1.75% by weight of bellflower extract, 1.75% by weight of pear extract, 1.75% by weight of pomegranate extract, and 1.75% by weight of perilla leaf extract with respect to the entire tea composition.
It was prepared by adding bellflower extract, pear extract, pomegranate extract and perilla leaf extract to fish collagen powder in a weight ratio of 1:1:1:1,
A collagen powder comprising 55% by weight of fish collagen powder, 1% by weight of bellflower extract, 1% by weight of pear extract, 1% by weight of pomegranate extract, and 1% by weight of perilla leaf extract with respect to the total collagen powder.
It was prepared by adding bellflower extract, pear extract, pomegranate extract and perilla leaf extract to fish collagen in a weight ratio of 1:1:1:1,
A collagen jelly comprising 5% by weight of fish collagen, 1% by weight of a bellflower extract, 1% by weight of a pear extract, 1% by weight of a pomegranate extract, and 1% by weight of a perilla leaf extract with respect to the total collagen jelly.

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