KR20110080998A - Composition for improving wrinkle and elasticity containing black currant anthocyanins - Google Patents

Abstract

PURPOSE: A composition containing black currant anthocyanin is provided to suppress collagenase activity and wrinkles and aging and to ensure elasticity. CONSTITUTION: A composition for preventing and treating skin wrinkling contains currant extract as an active ingredient. The currant extract is Ribes nigrum extract. The Ribes nigrum extract is black currant anthocyanins. An external use formulation contains the currant extract or black currant anthocyanine as an active ingredient. An inhibitor for collagenase contains the currant extract as an active ingredient.

Description

COMPOSITION FOR IMPROVING WRINKLE AND ELASTICITY CONTAINING BLACK CURRANT ANTHOCYANINS}

The present invention relates to a composition for improving and preventing skin wrinkles, comprising a natural product extract, specifically, a plant extract as an active ingredient.

The skin consists of an upper layer, an epidermis about 0.1 mm thick, a lower layer, a dermis and base layer about 1-4 mm thick. The causes of wrinkles of the skin appearance that are loosened due to loss of elasticity of the skin can be largely divided into the generation of wrinkles by natural aging and the generation of wrinkles by ultraviolet exposure (photoaging). The mechanism of wrinkle formation has not yet been accurately identified. Skin aging, which is closely related to the wrinkles, can also be classified into two types, including natural aging, ie, physiological aging over time and exposure to ultraviolet light, including wind, temperature, humidity, tobacco smoke, and pollution. There is extrinsic aging due to financial factors.

Common causes of these two wrinkles, namely, skin changes due to natural aging and photoaging, are skin elasticity due to the reduction and transformation of collagen and elastin, the major constituent proteins in the dermis, and the reduction of hyaluronic acid, the substrate of the dermis. Decrease. In particular, according to the research results so far, it is known that the reduction and modification of the main proteins collagen and elastin in the dermis are directly involved in the generation of wrinkles and the deterioration of skin elasticity.

The collagen is a major component of the extracellular matrix and is present in extracellular epilepsy as a major substrate protein produced in skin fibroblasts. It is also an important protein, accounting for about 30% of the total weight of biological proteins, and has a solid triple helix structure. Collagen forms most of the organic material of the skin, tendons, bones and teeth, especially in the dermis of bone and skin. Most other sieve structures exist as fibrous inclusions.

The main function of the collagen is to impart strength and tension to the skin to secure the mechanical strength of the skin, to induce connective tissue resistance, to bind tissue, to support cell adhesion, to divide and differentiate cells (when organic growth or wound healing). It is known as induction. In addition, collagen plays an important role in wound healing, and it is known that the wound can be quickly recovered without scar by promoting the synthesis of collagen in the damaged epithelium.

In particular, the reduction of collagen, which accounts for 90% of the dermis, is reported to be closely related to aging of the skin. More specifically, the synthesis and degradation of extracellular matrix such as collagen in vivo is properly regulated, but the progress of aging or UV irradiation promotes the expression of matrix metalloproteinase (MMP), an enzyme that degrades collagen. In addition, due to the collagen breakdown by the collagenase, the elasticity of the skin is lowered and wrinkles are formed.

In addition, there are various kinds of skin aging inhibitors known to date depending on the mechanism of skin aging inhibition. Representative examples include collagen production promoters, elastin activators, hyaluronic acid production increase agents, free radical scavengers, cell proliferators, photoaging inhibitors, keratinocyte proliferation inhibitors, humectants, harmful active oxygen production inhibitors, cell regeneration materials and the like.

In addition, a method of using an external composition for skin such as cosmetic or ointment containing collagen as a method for improving skin wrinkles has been proposed. This is suggested to take advantage of the wrinkle improvement and skin recovery effect of collagen, but it is difficult to absorb the percutaneous absorption of collagen, which is a high molecular material, can not expect a moisturizing effect or wound healing effect, so it is not possible to exhibit the essential skin function and wound healing function Pointed out.

It is proposed to solve the conventional problems, a method of preventing the formation of wrinkles by antioxidants such as vitamin C, vitamin E or carotenoids that inhibit the formation or reaction of free radicals, increasing collagen biosynthesis in the dermis such as retinol The method, the method of injecting collagen into the skin, or the method of injecting botulinum toxin into the skin has been proposed.

However, the vitamin C, vitamin E, carotenoids and retinol all have the disadvantage of being easily broken at room temperature, the retinol is known to have a disadvantage that the side effects on the skin when the treatment concentration is increased. In addition, the collagen or botulinum reading has been pointed out that the use of the skin is limited due to the safety problems such as irritation and redness, etc., due to the limitation of the amount used or the effect is insignificant, so that the effect of improving skin function or wound healing cannot be expected.

In order to solve the above problems, the present invention is safer in vivo than the material used as an active ingredient included in the existing wrinkle improving composition or the external preparation for improving wrinkles, the effect of improving skin wrinkles and elasticity, and less skin side effects It aims at providing the composition for skin wrinkle improvement and prevention containing.

In order to achieve the above object, the present invention provides a collagenase (collagenage) inhibitor containing the currant extract as an active ingredient.

In addition, the present invention provides a composition for improving and preventing skin wrinkles containing currant extract as an active ingredient in order to achieve the above object. The skin wrinkle improvement and prevention composition may be used as a medicine, food or cosmetics, the skin wrinkle improvement and prevention composition may be used as an external preparation.

The present inventors studied various natural ingredients to find a material that improves wrinkles and elasticity of the skin, and an anthocyanins contained in the extract of the currant seed, which is a natural ingredient, is present in the skin dermis. Inhibition of collagenase, an enzyme that breaks down collagen, a component of connective tissue, has been found to maintain skin elasticity, and in addition, experiments have been conducted using hairless mice. The present invention was confirmed that the anthocyanin contained in the extract of the currant seed has excellent skin anti-wrinkle effect, maintains the elasticity of the skin, inhibits the aging of the skin to maintain a young and smooth skin despite UV irradiation Was completed.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a collagenase inhibitor containing currant extract as an active ingredient.

In addition, the present invention relates to a composition for improving and preventing skin wrinkles containing currant extract as an active ingredient. The skin wrinkle improvement and prevention composition may be used as a medicine, quasi-drug, food or cosmetics. In addition, the composition for improving and preventing skin wrinkles can be used as an external preparation.

In the present invention, the currant means a deciduous shrub belonging to the genus Rosaceae. The currant Europe and Central Asia have called wonsanjiyi you reverse the color of the fruit is black then black currant or black currant (black currant) Groom (Ribes nigrum ), distributed in Europe and growing to about 1 m, red currant with bisexual flowers in April-May, berry fruits in July-August, distributed in Korea and northeast China, Currants or sanaeng also called called, grew up in the highlands, 5 to 6 defendants are bisexual in red mauve flowers in May, with chapters and in October the ball-shaped, red ripe berries temperature may cause problems, create reverse Shree Com (Ribes mandshuricum ), Black Currant or Black Currant, is distributed in northern Korea and Sakhalin Island, grows under high mountain trees, white-green green or yellow bisexual flowers bloom in spring, Ribes with round, black ripe berries ( Ribes) ussuriense ). The fruit of the currant has been used for food, such as jam or juice or jelly, or medicinal. The term "currant" is also used to mean 'small seedless raisins'.

In the present invention, anthocyanin is a water-soluble pigment glycoside, which is often present in a flower, fruit, stem, leaf, or root of a plant and is composed of aglycone and glucoside, and is used for hydrolysis. By one or two monosaccharides and aglycone. Often, it is a natural pigment present in the vacuoles of a plant and has a color such as purple, red, or blue depending on the acid concentration of the cell solution, the chemical structure of the new compound, and the bonding state with various metal ions.

In the present invention, collagenase is an enzyme that digests collagen, which may be, for example, MMP-1, but is not limited thereto.

The currant of the present invention may be a reverse nigrum, preferably a fruit of reverse nigrum. The anthocyanins of the present invention may also be anthocyanins of currant, preferably anthocyanins or blackcurrant anthocyanins of reverse nigrum.

The currant extract of the present invention is preferably a reverse nigrum (Ribes nigrum) extract, more preferably an anthocyanin or black current anthocyanin of the reverse nigrum.

The currant extract of the present invention can be prepared by extracting or extracting and fractionating the currant using the extraction solvent or the extraction solvent and fraction solvent.

The extraction solvent may be water, a polar organic solvent, a non-polar organic solvent or a mixed solvent thereof. The polar organic solvent may be an alcohol having 1 to 5 carbon atoms such as methanol, ethanol, ethyl acetate, acetone, and the like, and the nonpolar organic solvent may be hexane, chloroform, methylene chloride or dichloromethane. The extraction solvent may be at least one selected from the group consisting of water and alcohols having 1 to 5 carbon atoms, and more preferably an aqueous alcohol solution.

The fractional solvent may be water or an organic solvent such as a polar solvent such as alcohol having 1 to 5 carbon atoms such as methanol or ethanol, ethyl acetate, and a nonpolar solvent of hexane or dichloromethane, or a mixed solvent thereof. For example, the polar solvent or nonpolar solvent may be an alcohol having 1 to 5 carbon atoms, hexane, chloroform, ethyl acetate, acetone, methylene chloride, dilution water thereof, or a mixture thereof.

The method of manufacturing the currant extract of the present invention may be to add an extraction solvent to the currant or currant extract of the currant and then perform the extraction process.

The extraction process may be an ultrasonic extraction method or a device extraction method. In the ultrasonic extraction method, for example, 500 to 1,000 ml of an 80% methanol aqueous solution is added based on 200 g of blackcurrant fruit, and then pulverized, for 5 to 90 minutes, preferably 10 to 60 minutes, and more preferably 15 to 30 minutes. While ultrasonic extraction can be performed. In addition, the blackcurrant crushed sample may be prepared by drying the blackcurrant washed with running water and then crushing with a grinder.

In addition, in the preparation of the extract, after performing the extraction method, the extract is filtered using a filter cloth, the residue is extracted one or more times in the same manner as the extraction method, and the step of combining with the first obtained primary extract It can be carried out further to prepare.

The extract may be prepared by performing a filtration process or concentrated and / or lyophilization for the crude extract prepared by performing the obtained extraction process. The filtration process may remove residues left in the extract, and may be concentrated and / or lyophilized to remove moisture. In addition, the concentration may be carried out using a vacuum concentrator, the vacuum concentration process may be carried out under the conditions of 30 to 70 ℃, preferably 40 to 50 ℃.

The method of extracting or separating and refining the anthocyanin or black currant anthocyanin of the reverse nigrum may be any method of separating and purifying anthocyanin from a plant or plant extract known to those skilled in the art. It is not limited to any particular method.

For example, it may be a chromatography method using the developing solvent or a method using a nonionic polymeric absorbent column. When the adsorbent column is used, the anthocyanin can be eluted from the adsorbent using methanol and separated using ethyl acetate.

The currant extract or blackcurrant anthocyanin prepared by the above method not only inhibits collagenase (MMP-1) of human-derived fibroblasts, but also improves skin wrinkles as a result of experiments using experimental animals. Since it is possible to prevent aging of the skin, it has been confirmed that the effect of preventing or improving skin wrinkles and improving the elasticity of the skin is confirmed.

Therefore, the composition for improving and preventing wrinkles comprising the currant extract or black currant anthocyanin as an active ingredient may be applied as an anti-wrinkle medicine or an anti-aging drug, quasi-drug, food or external preparation, and examples of the external preparation include skin It may be a cosmetic composition for wrinkle improvement and prevention. All simple modifications or changes of the present invention can be easily carried out by those skilled in the art, and all such modifications or changes are included in the scope of the present invention.

In addition, the collagenase inhibitor comprising the currant extract or black currant anthocyanin of the present invention as an active ingredient may be applied as an anti-wrinkle or anti-aging drug, quasi-drug, food or external preparation.

The present invention may be a pharmaceutical composition for improving and preventing wrinkles comprising currant extract or black currant anthocyanin as an active ingredient.

In addition, the present invention may be a food composition for improving and preventing wrinkles comprising currant extract or black currant anthocyanin as an active ingredient.

In addition, the present invention may be an external preparation for improving and preventing wrinkles comprising currant extract or black currant anthocyanin as an active ingredient.

Wrinkle improvement or preventive pharmaceutical composition comprising the black currant anthocyanin as an active ingredient can be directly applied to animals including humans. The animal is a biological group corresponding to plants, and mainly refers to a nutrient ingested from organic matter, digested, embryogenic, and respiratory organs are differentiated, preferably mammals, and more preferably humans.

Wrinkle improvement and prevention pharmaceutical composition comprising the currant extract or the black currant anthocyanin as an active ingredient may include the currant extract or the black currant anthocyanin alone, in addition to the formulation, method and purpose of use It may further comprise a pharmaceutically acceptable carrier, excipient or diluent.

When the currant extract or black currant anthocyanin is provided as a mixture in addition to the currant extract or black currant anthocyanin as an active ingredient in the pharmaceutical composition for improving and preventing wrinkles comprising the currant extract or the black currant anthocyanin, the wrinkle improvement And 0.001% to 99.9% by weight of the currant extract or the black currant anthocyanin, preferably 0.1% to 99% by weight, and more preferably 1% to 50% by weight, based on the total weight of the prophylactic pharmaceutical composition. can do.

When the pharmaceutical composition for improving and preventing wrinkles comprising the currant extract or the black currant anthocyanin as an active ingredient is used as a medicament, the administration method may be oral or parenteral, for example, oral, transdermal, subcutaneous, intravenous or Administration can be via several routes, including muscle.

In addition, the formulation of the composition may vary depending on the method of use and may be formulated by employing methods known in the art to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal. Can be.

Generally, solid preparations for oral administration include tablets, pills, soft or hard capsules (CAPSULES), pills (PILLS), powders (POWDERS) and granules (GRANULES), and the like. Excipients, for example, may be prepared by mixing starch, calcium carbonate, sucrose or lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral use include suspensions, liquids, limonades, emulsions, and syrups. In addition to commonly used simple diluents such as water and liquid paraffin, Excipients such as wetting agents, sweetening agents, fragrances, preservatives and the like can be included.

In addition, for parenteral administration, creams, lotions, creams, ointments, eye creams, warnings, liquids, aerosols, fluids, etc. (FRUIDEXTRACTS), ELIXIR, INFUSIONS, SACHET, LINIMENTS, PATCH or INJECTIONS.

Furthermore, the compositions of the present invention may be formulated using suitable methods known in the art or using methods disclosed in Remington's Pharmaceutical Science (Recent Edition, Mack Publishing Company, Easton PA). Can be.

Pharmaceutically acceptable carriers, excipients or diluents according to the formulation, method of use and purpose of use are lactose, dextrose, sucrose, sorbitol, mannitol, xyitol, erythritol, maltitol, starch, akicia rubber, alginate, gelatin, calcium Phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, dextrin, calcium carbonate, Propylene glycol, liquid paraffin, may be one or more selected from the group consisting of physiological saline, but is not limited thereto, conventional carriers, excipients or diluents can be used.

In addition, when formulating a pharmaceutical composition for improving and preventing wrinkles comprising the currant extract or the black currant anthocyanin as an active ingredient, conventional fillers, extenders, binders, disintegrants, surfactants, anticoagulants, lubricants, wetting agents, It may further include fragrances, emulsifiers or preservatives, and can be used orally or parenterally, but preferably for oral administration. Specifically, solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations include at least one excipient such as starch, calcium carbonate, and sucrose in the extract. (sucrose), lactose (lactose), gelatin, etc. are mixed and prepared. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral use include suspensions, solvents, emulsions, and serums.In addition to commonly used simple diluents such as water and liquid paraffin, various excipients include, for example, wetting agents, sweeteners, fragrances, and preservatives. Can be.

The dose of the currant improvement and prevention pharmaceutical composition comprising the currant extract or the black currant anthocyanin according to the present invention as an active ingredient, taking into account the method of administration, the age, sex and weight of the recipient, and the severity of the disease, etc. It may be appropriately selected by those skilled in the art.

As an example, the anti-wrinkle pharmaceutical composition comprising the currant extract or the black currant anthocyanin of the present invention as an active ingredient is 0.0001 mg / kg (based on the content of the active ingredient per day) Body weight) to 1000 mg / kg body weight, and to be more effective, 0.01 mg / kg body weight to 100 mg / kg body weight. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

In addition, when the pharmaceutical composition for wrinkle improvement and prevention containing the currant extract or the black currant anthocyanin as an active ingredient is administered parenterally or externally, it is active in the age, sex, condition and body of the subject to which the composition is applied. It can be applied differently depending on the absorbency of the ingredients, the inactivation rate, the type of drug or cosmetics used together, for example, 1 ml per day, 4 cm ² (0.25 ml / cm ² skin) (black currant anthocyanin 1 μg / ml water) It may be administered to the skin, but is not limited thereto. The invention also includes formulations of dosage units.

In addition, the currant improvement and prevention pharmaceutical composition comprising the currant extract or the black currant anthocyanin as an active ingredient, in addition to the currant extract or the black currant anthocyanin, known wrinkle improvement and prevention effect or skin aging effect It may further include a compound or plant extract having, and may be included in 5 parts by weight to 20 parts by weight based on 100 parts by weight of the currant extract or the black currant anthocyanin.

In addition, the present invention relates to a food composition for improving and preventing wrinkles comprising the currant extract or the black currant anthocyanin as an active ingredient. Examples of the food composition of the present invention include food, food additives, beverages or beverage additives.

Wrinkle improvement and prevention food composition comprising the currant extract or the black currant anthocyanin as an active ingredient may further comprise a suitable carrier, excipients and diluents commonly used in the preparation thereof.

In the present specification, the term "food" means a natural product or processed product containing one or more nutrients, and preferably means a state in which it can be directly eaten through some processing process. It is intended to include all foods, food additives, health functional foods and beverages.

Examples of the food which may include the currant extract or the black currant anthocyanin as an active ingredient of the food composition of the present invention include various foods, beverages, gums, teas, vitamin complexes, functional foods, and the like. In addition, the food in the present invention includes special nutritional products (e.g., prepared oils, infants, baby food, etc.), processed meat products, fish products, tofu, jelly, noodles (e.g. ramen, noodles, etc.), health supplements, seasoned foods ( For example, soy sauce, miso, red pepper paste, mixed soy sauce), sauces, confectionery (e.g. snacks), dairy products (e.g. fermented milk, cheese, etc.), other processed foods, kimchi, pickles (various kimchi, pickles, etc.), beverages ( Examples include, but are not limited to, fruits, vegetable drinks, soy milk, fermented beverages, ice cream, etc., natural seasonings (eg, ramen soup, etc.), vitamin complexes, alcoholic beverages, alcoholic beverages, and other health supplements. The food, beverage or food additive may be prepared by a conventional production method.

Functional food in the present invention is the control of biological defense rhythm, disease prevention and recovery of food groups or food compositions that have added value to the food by using physical, biochemical, biotechnological techniques, etc. It refers to a food processed by designing so as to sufficiently express the function of the gymnastics for the back, and more specifically, the function of preventing skin aging or preventing or improving skin wrinkles. The functional food may include food acceptable food additives, and may further include appropriate carriers, excipients and diluents commonly used in the manufacture of functional foods.

In the present invention, the drink is a generic term for drinking to quench thirst or to enjoy the taste and is intended to include a functional drink. The beverage is not particularly limited in addition to the currant extract or the black currant anthocyanin as an active ingredient in the indicated ratios as an active ingredient, and additional ingredients such as various flavors or natural carbohydrates, such as ordinary drinks. It may contain as. Examples of such natural carbohydrates include monosaccharides such as glucose, fructose and other disaccharides such as maltose, sucrose and the like and polysaccharides such as dextrin, cyclodextrin and the like, and Sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The ratio of the natural carbohydrate may generally be about 1 to 20 g, preferably 5 to 12 g per 100 ml of the composition of the present invention. It may further contain a pulp for.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. These components can be used independently or in combination. The additive may be 0 to 20 parts by weight, preferably 0.00001 to 15 parts by weight, per 100 parts by weight of the currant extract or the black currant anthocyanin, which is an active ingredient of the present invention, but is not limited thereto.

Functional beverage in the present invention is a biological defense rhythm control, disease prevention and the like having a beverage group or a beverage composition that has added value to the beverage by using physical, biochemical, biotechnological techniques, etc. to function and express the function of the beverage to a specific purpose Means a beverage that is designed and processed to fully express the gymnastics function related to recovery.

The functional beverage is not particularly limited to other ingredients other than containing the currant extract or the black currant anthocyanin, which are the active ingredients of the present invention as essential ingredients in the indicated ratios, and various flavors or natural carbohydrates, such as ordinary drinks. May be contained as an additional component. Examples of such natural carbohydrates include monosaccharides such as glucose, fructose and other disaccharides such as maltose, sucrose and the like and polysaccharides such as dextrin, cyclodextrin and the like, and xylitol Sugar alcohols such as sorbitol and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The natural carbohydrate may be included in an amount of 0 to 20 parts by weight, preferably 1 to 18 parts by weight, more preferably 5 to 12 parts by weight, per 100 parts by weight of the composition of the present invention.

In addition, in the anti-wrinkle food composition comprising the currant extract or the black currant anthocyanin as an active ingredient, the amount of the currant extract or the black currant anthocyanin is 0.00001% to 50% by weight of the total food weight The beverage composition may be included in a ratio of 0.001 g to 50 g, preferably 0.01 g to 10 g, based on 100 ml of the total volume of the food, but is not limited thereto.

In addition, the present invention provides a topical agent for improving and preventing wrinkles, characterized by containing the currant extract or the black currant anthocyanin.

In the present invention, the external preparation is a concept encompassing the general composition used for external use, and is widely used in various medicines or quasi-drugs, such as cosmetic compositions and ointments including various cosmetics such as basic cosmetics, makeup cosmetics, and hair cosmetics. Say what is applicable.

In addition to the active ingredient, the external preparation may be a component that is usually formulated in the external preparation, for example, a moisturizer, an ultraviolet absorber, vitamins, animal and plant extracts, a digestive agent, a whitening agent, a vasodilator, an astringent, a cooling agent, and a hormonal agent. It may be further formulated.

The preparation of the external preparation may take an appropriate form depending on the purpose of use and the nature of the external preparation composition. For example, an aqueous solution, a solubilizing system, an emulsion, an emulsion, a gel, a paste, an ointment, an aerosol, and an oil-based oil 2 It may be a layered, water-oil-powder three-layered, and the formulation and form of the external preparation of the present invention is not limited by the formulation.

In addition, the external preparation may further include a base necessary for infiltrating or transferring the active ingredient into skin tissue.

The active ingredient may be included in an amount of 0.01 to 30% by weight based on the total weight of the external preparation for improving wrinkles and preventing wrinkles, preferably 0.05 to 20% by weight, but the content of the ingredient other than the active ingredient contained in the formulation or external preparation. It can adjust suitably according to content. When the content thereof is less than 0.01% by weight, it may be difficult to obtain the expected effect. When the content thereof exceeds 30% by weight, the stability of the product may be due to the strong dispersibility of the blackcurrant extract or the black currant anthocyanin. It can have a bad effect.

As an example of the present invention, the cosmetic composition for skin wrinkle improvement and prevention containing the currant extract or the black currant anthocyanin as an active ingredient, in addition to the active ingredient, all kinds of ingredients usable in ordinary cosmetics, such as perfumes, Colorants, fungicides, antioxidants, preservatives, moisturizers, thickeners, excipients, diluents, inorganic salts and synthetic polymeric materials may further be included.

The cosmetic composition may be provided for the use of basic cosmetics, makeup cosmetics, body cosmetics, hair cosmetics, scalp cosmetics, shaving cosmetics or oral cosmetics. Examples of the basic cosmetics include creams, lotions, packs, massage creams, emulsions, etc. The makeup cosmetics include foundation, makeup base, lipstick, eyeshadow, eyeliner, mascara, eyebrow pencil, and the like. Examples include soaps, liquid cleansers, baths, sunscreen creams and sun oils. Hair care products include shampoos, rinses, hair treatments, hair mousses, hair liquids, pomades, hair colorants, hair bleaches, and colorants. There are rinses, hair tonics and scalp treatments as scalp cosmetics, aftershave and shaving creams as shaving cosmetics, and toothpaste and mouth washes as oral cosmetics.

The composition of the present invention is excellent in the inhibitory activity of collagenase (MMP-1), prevents skin aging by ultraviolet rays, prevents and improves skin wrinkles, as well as excellent skin and moisture retention effect, edible Since it is manufactured from natural plants used as, there is no problem in safety, and it may be useful to improve or prevent skin aging and skin wrinkles. In particular, the black currant anthocyanin of the present invention, due to the securing of such excellent effects and safety, for example, an external composition, for the treatment, improvement of various skin-related diseases, including functional cosmetics or skin anti-aging and skin wrinkle improvement Or it may be added to the prophylactic drug or quasi-drugs, the kind of such functional cosmetics or drugs or quasi-drugs is not particularly limited.

In addition, the present invention provides a method for producing black currant anthocyanin having a skin wrinkle improvement and prevention effect.

The method for producing black currant anthocyanin having an effect of improving and preventing skin wrinkles includes: pulverizing by adding an aqueous alcohol solution to the black currant fruit and pulverizing it; Preparing the extract by extracting the pulverized pulverized liquid for 10 minutes to 30 minutes, preferably 15 minutes to 25 minutes by ultrasonic extraction; Preparing an extract by removing the solvent of the extract; The extract was separated using a nonionic polymeric absorbent column, eluted with methanol, followed by removal of methanol to obtain crude anthocyanin, and the crude anthocyanin obtained above dissolved in methanol, followed by ethyl acetate. It may be to include the step of obtaining and fractionating to obtain anthocyanin.

The preparation method may further include purifying anthocyanin by performing chromatography using an HPLC column of the anthocyanin obtained by adding and fractionating the ethyl acetate.

The black currant anthocyanin prepared by the above method is excellent in collagenase inhibitory activity, not only an anti-aging effect of skin, but also an excellent anti-wrinkle effect, which is useful for preventing and improving skin wrinkles or preventing skin aging. There is an advantage that it can be used.

As described above, the currant extract and the black currant anthocyanin are natural ingredients, have no side effects on the skin, have excellent collagenase inhibitory activity, a collagen degrading enzyme, and skin wrinkles even when irradiated with ultraviolet rays as confirmed by experimental animals. It is expected to improve the wrinkles and elasticity of the skin by preventing the aging and preventing skin aging, and considering the fact that it can be increased because it is safe for the human body as a natural product, various foods and medicines for the purpose of improving and preventing skin wrinkles It can be applied variously as a raw material of an external composition including cosmetics.

1 is in accordance with an embodiment of the present invention, in order to confirm the collagenase (MMP1) inhibitory effect of the black currant anthocyanin, collagenase (MMP1) and collagen protein expression in a comparative analysis picture, No. 1 represents the control group, No. 2 was administered ATRA 0.1 / ml, No. 3 was administered ATRA 1 / ml, No. 4 was administered ATRA 10 / ml, and No. 5 was BCP 1 / ml 6 was administered BCP 10 / ml, 7 times was administered BCP 100 / ml, 8 was administered SBA 1 / ml, and 9 was administered SBA 10 / ml 10 times the administration of SBA 100 / ml, 11 times the administration of BCA 1 / ml, 12 times the administration of BCA 10 / ml, 13 times the administration of BCA 100 / ml , 14 is the administration of PCP 1 / ml, 15 is the administration of PCP 10 / ml, 16 Is PCP 100 / ml, 17 times HP 1 / ml, 18 times HP 10 / ml, 19 times HP 100 / ml, 20 times SBP 1 / ml was administered, the 21 was administered SBP 10 / ml, the 22 was administered SBP 100 / ml, the ATRA positive control group means all trans retinoic acid, the BCP is black Current Polyphenol means, SBA means Saskatoon Berries Anthocyanins, BCA means Black Currant Anthocyanins, the active ingredient of the present invention, PCP means Purple Corn Polyphenols, HP Means Honeysuckle Polyphenols, and SBP means Saskatoon Berries Polyphenols.
FIG. 2 illustrates the improvement of skin wrinkles when black currant anthocyanin is treated on the back of a hairless mouse induced by UV irradiation in order to confirm the skin wrinkle prevention effect of black currant anthocyanin according to an embodiment of the present invention. It is a picture showing.
FIG. 3 is a visioscan analysis of skin treated with black currant anthocyanin on the back of a hairless mouse induced by UV irradiation in order to confirm the anti-wrinkle effect of black currant anthocyanin according to an embodiment of the present invention. As a result, it is a graph that shows the value calculated by comparing the surface size to the side.
FIG. 4 is a visioscan analysis of skin treated with black currant anthocyanin on the back of a hairless mouse induced by UV irradiation in order to confirm the anti-wrinkle effect of black currant anthocyanin according to an embodiment of the present invention. As a result, it is a graph that calculates the total amount of liquid required until the image fills the average height of all the curves.
5 is a visioscan of skin when black currant anthocyanin is treated on the back of a hairless mouse induced by ultraviolet irradiation to confirm the skin wrinkle prevention effect of black currant anthocyanin according to an embodiment of the present invention. One result, the NRJ value, is a graph showing the elastic energy value.
6 is a visioscan of skin when black currant anthocyanin is treated on the back of a hairless mouse induced by UV irradiation to confirm the skin wrinkle prevention effect of black currant anthocyanin according to an embodiment of the present invention. The result is a graph showing the difference between two gray levels that are adjacent to the resulting contrast value.
7 is a visioscan of skin when black currant anthocyanin is treated on the back of a hairless mouse induced by UV irradiation to confirm the skin wrinkle prevention effect of black currant anthocyanin according to an embodiment of the present invention. As a result, the Variance value is the average value of the subsurface height.

Hereinafter, the present invention will be described in detail with reference to Examples. However, the following examples may be modified in many different forms, which are intended to illustrate the present invention, but the protection scope of the present invention is not limited to the following examples.

[Example]

Example  1. Black Currant  Anthocyanins ( Black currant Anthocyanins Extraction and separation

To 200 g of black currant, 80 ml of 80 aquous methanol (0.1% formic acid) is added, ground in a food processor, and sonicated for 20 minutes. The supernatant is collected by centrifuging the extract at 1,000 ° C. for 30 min at 4 ° C. 70 ml of 1: 4 H ₂ O-Methanol mix (0.1% formic acid) is added to the precipitate, and the supernatant is collected in the same manner and combined with the primary supernatant. The supernatant is evaporated at 40 ° C. using a vacuum evaporator.

The residue obtained by evaporating the solvent is dissolved in water (0.1% formic acid) and poured into a nonionic polymeric absorbent (Amberlite XAD-16) column. After thorough washing with water (0.1% formic acid), anthocyanin is eluted with methanol (0.1% formic aci) d and the solvent is removed from the rotary evaporator.

The solvent is removed and the residue obtained is dissolved in methanol (0.1% formic acid) and anthocyanin is separated with ethyla acetate. The anthocyanins thus obtained were separated by gradient elutiuon between 4.5% formic acid (A) and 80% acetonitrile (B) using a reversed-phase C-18 HPLC column.

Example  2. Human fiber In culturing Collagen , Collagenase  Wrinkle prevention and improvement analysis through expression analysis

In order to investigate the expression of collagen I and collagenase I, which are indicator proteins of wrinkles, human embryonic fibroblasts obtained in this laboratory were cultured with trypsin-EDTA at a dilution of 1: 4 (at cleavage number 2). Change to a new Petri dish (diameter 60mm) and continue to increase the number, subcultured in large quantities, when the number of cell division reaches 20 (with no aging characteristics), add 10% DMSO Freezer stock was made from 1 vial and stored in liquid nitrogen.

The culture of fibroblast using DMEM 10% fetal bovine serum (including penicillin-streptomycin) as a medium, the medium was changed every 3 days at 37 ℃, 5% CO ₂ .

The extracts were treated in different concentrations (1, 10, 100 / ml) of young human embryonic fibroblasts with a population of doubling (PD) 30. After 48 hours, the medium was collected and subjected to Acetone precipitation. Protein expression of procollagen and MMP I (collagenase I) was measured using Western blot analysis using antibodies.

 Each extract was treated with human embryonic fibroblasts and then protein expression was examined by acetone precipitation of the medium of human embryonic fibroblasts in the treated and non-treated groups with NET buffer (150 mM NaCl, 50 mM Tris-HCl pH7.4, 5 mM EDTA). pH 8.0) is used to lyse pellet and quantify by Lowry method. After electrophoresis at 150 V for 1 hour on SDS PAGE gel, transfer 70 V to the transfer membrane for 30 minutes, soak the membrane in non-fat milk solution and react overnight.

After washing 3 times with TBST washing solution for 10 minutes, immunoblotting for 2 hours using the primary antibody, then washed 3 times with TBST washing solution, and then immunoblotting with secondary antibody for 30 minutes, again 3 After washing twice, the expression of the protein was examined by using ECL kit on the film. The results are shown in FIG.

As shown in FIG. 1, in the case of the black currant anthocyanin of the present invention, it exhibits better MMP-1, that is, collagenase inhibitory activity, which is a collagen degrading enzyme, at the same content as ATRA, which is a positive control group, and is excellent in preventing wrinkles. Confirmed.

Example 3: Wrinkle improvement efficacy measurement using hairless mice

In vivo models using hairless mice have been accepted as the main method for performing skin wrinkle studies. In the following examples, the wrinkle prevention and improvement effect of black currant anthocyanins was identified through in vivo experiments using hairless mice.

The embodiment of the method for purifying Black currant Anthocyanins from plants and identifying the chemical structure of the purified material to prove that it is Black currant Anthocyanins is not a new method and any other separation Purification and structural analysis are also possible.

The animals used in the experiments were purchased for 5 weeks of hairless mice, stabilized for 1 week, and tested for 8 weeks. During the experimental period, the room environment was maintained at 23 ± 2 ℃ and relative humidity 55 ± 10%. Five animals were kept in cages for mice under time artificial lighting, and feed and drinking water for experimental animals were freely supplied.

Skin wrinkles were induced by UV irradiation for 8 weeks to form wrinkles. Minimal erythema (Minimun Erythema Dose, MED) was measured using a UV lamp (Philips TL20w / 01RS, USA) to determine 1MED as 120mJ / cm ² . Three times a week, the dose was started at 1MED (120mJ / cm ² ) in the first week, increased to 3MED at 2MED in 3 weeks, and 3MED at 3 weeks or more.

The experimental group consisted of 5 UV controls (exposed to UV only), ATRA treated group (positive control group, All trans-retinoic acid, 1μM, material already used as a wrinkle improver in cosmetics), # 3 (Black currant anthocyanins) 0.5%, # 6 (Saskatoon berries polyphenols) divided by 0.5% was tested.

ATRA, # 3, # 6 was used by dissolving in the cosmetic base. Apply each substance to the back 5 times a week, and after 8 weeks of treatment, measure the wrinkles of the skin. After breathing the hairless rat using Visioscan (VC 98), take a close contact with the back skin and take a picture. The values were evaluated using the results, and the results are shown in FIGS. 2 to 7.

As shown in FIG. 2 to FIG. 7, the black currant anthocyanin of the present invention exhibited a wrinkle improvement and prevention effect comparable to that of ATRA, which is a positive control group. In FIG. Many have been confirmed. In addition, in the case of Figure 4, a smaller value than ATRA was confirmed to be less wrinkles, and in Figure 5 was confirmed that a high value similar to ATRA, the skin is young, moist and elastic despite UV irradiation. 6 and 7 were similar to ATRA and showed significantly lower values than Saskatoon berries polyphenols, indicating that less skin wrinkles occurred.

Claims (5)

Skin wrinkle improvement and prevention composition containing currant extract as an active ingredient. The method of claim 1,
The currant extract is a reverse nigrum (Ribes nigrum) extract, characterized in that the composition for skin wrinkle improvement and prevention. The method of claim 2,
The reverse nigrum (Ribes nigrum) extract is a composition for improving and preventing skin wrinkles, characterized in that the black currant anthocyanin. External preparation for improving and preventing skin wrinkles comprising currant extract or black currant anthocyanin as an active ingredient. Collagenase inhibitor containing currant extract as an active ingredient.

Images