KR102409738B1 - 슈퍼-해상도 이미징 - Google Patents

슈퍼-해상도 이미징 Download PDF

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KR102409738B1
KR102409738B1 KR1020187032632A KR20187032632A KR102409738B1 KR 102409738 B1 KR102409738 B1 KR 102409738B1 KR 1020187032632 A KR1020187032632 A KR 1020187032632A KR 20187032632 A KR20187032632 A KR 20187032632A KR 102409738 B1 KR102409738 B1 KR 102409738B1
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light
sample
delete delete
waveguide
super
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KR20180130567A (ko
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발프레트 싱 알루발리아
마르크 슈트펠즈
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유니벨시테테트 이 트롬소 - 노르게스 알크티스키 유니벨시테트
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/56—Optics using evanescent waves, i.e. inhomogeneous waves
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/02—Optical fibres with cladding with or without a coating
    • G02B6/02004—Optical fibres with cladding with or without a coating characterised by the core effective area or mode field radius
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/02—Optical fibres with cladding with or without a coating
    • G02B6/02042—Multicore optical fibres
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/10—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type
    • G02B6/12—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type of the integrated circuit kind
    • G02B6/122—Basic optical elements, e.g. light-guiding paths
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T3/00—Geometric image transformations in the plane of the image
    • G06T3/40—Scaling of whole images or parts thereof, e.g. expanding or contracting
    • G06T3/4053—Scaling of whole images or parts thereof, e.g. expanding or contracting based on super-resolution, i.e. the output image resolution being higher than the sensor resolution
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/10—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type
    • G02B6/12—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type of the integrated circuit kind
    • G02B2006/12083—Constructional arrangements
    • G02B2006/12102—Lens

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Health & Medical Sciences (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Theoretical Computer Science (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Optical Measuring Cells (AREA)
KR1020187032632A 2016-04-12 2017-04-12 슈퍼-해상도 이미징 Active KR102409738B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1606268.9 2016-04-12
GB1606268.9A GB2549298B (en) 2016-04-12 2016-04-12 Super-resolution imaging
PCT/GB2017/051034 WO2017178823A1 (en) 2016-04-12 2017-04-12 Super-resolution imaging

Publications (2)

Publication Number Publication Date
KR20180130567A KR20180130567A (ko) 2018-12-07
KR102409738B1 true KR102409738B1 (ko) 2022-06-15

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Country Status (11)

Country Link
US (1) US10983326B2 (https=)
EP (1) EP3443401B1 (https=)
JP (1) JP6997928B2 (https=)
KR (1) KR102409738B1 (https=)
CN (1) CN109313330B (https=)
AU (1) AU2017248718B2 (https=)
CA (1) CA3020529C (https=)
DK (1) DK3443401T3 (https=)
ES (1) ES2929874T3 (https=)
GB (1) GB2549298B (https=)
WO (1) WO2017178823A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3839483B1 (en) * 2019-12-19 2023-07-26 Imec VZW An illuminator, an imaging apparatus and a system
AU2021207899A1 (en) 2020-01-14 2022-09-08 Quantum-Si Incorporated Waveguide excitation uniformity
CN111722392B (zh) * 2020-07-22 2021-06-08 南京大学 一种基于超构透镜阵列的大视场集成显微成像装置
KR102619825B1 (ko) 2020-10-20 2024-01-03 한양대학교 산학협력단 초고해상도 형광 현미경용 이미징 버퍼 용액 및 이를 이용한 초고해상도 형광 현미경 이미징 방법
KR102654655B1 (ko) 2020-10-20 2024-04-05 한양대학교 산학협력단 초고해상도 형광 현미경 이미징 방법 및 초고해상도 형광 현미경 이미징 장치
CN113031243B (zh) * 2021-03-26 2021-08-31 杭州辰景光电科技有限公司 一种基于波导片的反射式片上数字全息显微装置
DE102021131952A1 (de) * 2021-12-03 2023-06-07 Interherence GmbH Optisches Modul
US12536611B2 (en) * 2021-12-08 2026-01-27 Bar Ilan University Method and system for super-resolved imaging
EP4220269A1 (en) * 2022-01-27 2023-08-02 Abbelight Light imaging device
KR102742598B1 (ko) * 2022-02-10 2024-12-16 한양대학교 산학협력단 형광현미경 또는 초고해상도 형광현미경을 이용한 용매의 수소 결합 센싱 방법
CN116297377B (zh) * 2023-05-22 2023-07-28 深圳高性能医疗器械国家研究院有限公司 单分子荧光检测芯片及其制备方法、单分子荧光检测仪

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US20030058440A1 (en) * 2001-08-28 2003-03-27 Scott Graham B. I. Pulsed-multiline excitation for color-blind fluorescence detection
US20090237501A1 (en) 2008-03-19 2009-09-24 Ruprecht-Karis-Universitat Heidelberg Kirchhoff-Institut Fur Physik method and an apparatus for localization of single dye molecules in the fluorescent microscopy
US20120062722A1 (en) * 2010-06-11 2012-03-15 Nikon Corporation Microscope apparatus and observation method
US20120092477A1 (en) * 2010-10-18 2012-04-19 Olympus America Inc. Wide field microscopic imaging system and method
US20120293797A1 (en) * 2009-12-17 2012-11-22 Universiteit Gent Methods and systems for optical characterisation
JP2013524174A (ja) 2010-06-11 2013-06-17 インダストリアル テクノロジー リサーチ インスティテュート 単一分子検出装置
US20130256563A1 (en) * 2012-03-28 2013-10-03 Carl Zeiss Microscopy Gmbh High-resolution fluorescence microscopy
WO2016006006A1 (en) 2014-07-09 2016-01-14 Ntp Nano Tech Projects S.R.L. Laser optical coupling for nanoparticles detection

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US7776613B2 (en) * 2006-08-07 2010-08-17 President And Fellows Of Harvard College Sub-diffraction image resolution and other imaging techniques
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EP2801854B1 (en) * 2013-05-10 2017-07-19 Ruprecht-Karls-Universität Heidelberg Method and apparatus for combination of localization microscopy and structured illumination microscopy
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US20030058440A1 (en) * 2001-08-28 2003-03-27 Scott Graham B. I. Pulsed-multiline excitation for color-blind fluorescence detection
US20090237501A1 (en) 2008-03-19 2009-09-24 Ruprecht-Karis-Universitat Heidelberg Kirchhoff-Institut Fur Physik method and an apparatus for localization of single dye molecules in the fluorescent microscopy
US20120293797A1 (en) * 2009-12-17 2012-11-22 Universiteit Gent Methods and systems for optical characterisation
US20120062722A1 (en) * 2010-06-11 2012-03-15 Nikon Corporation Microscope apparatus and observation method
JP2013524174A (ja) 2010-06-11 2013-06-17 インダストリアル テクノロジー リサーチ インスティテュート 単一分子検出装置
US20120092477A1 (en) * 2010-10-18 2012-04-19 Olympus America Inc. Wide field microscopic imaging system and method
US20130256563A1 (en) * 2012-03-28 2013-10-03 Carl Zeiss Microscopy Gmbh High-resolution fluorescence microscopy
WO2016006006A1 (en) 2014-07-09 2016-01-14 Ntp Nano Tech Projects S.R.L. Laser optical coupling for nanoparticles detection

Also Published As

Publication number Publication date
CN109313330A (zh) 2019-02-05
JP6997928B2 (ja) 2022-01-18
DK3443401T3 (da) 2022-10-24
KR20180130567A (ko) 2018-12-07
EP3443401A1 (en) 2019-02-20
CN109313330B (zh) 2021-06-15
JP2019516140A (ja) 2019-06-13
CA3020529C (en) 2023-10-17
ES2929874T3 (es) 2022-12-02
GB2549298A (en) 2017-10-18
EP3443401B1 (en) 2022-07-20
GB2549298B (en) 2022-02-02
US10983326B2 (en) 2021-04-20
US20190129161A1 (en) 2019-05-02
CA3020529A1 (en) 2017-10-19
AU2017248718B2 (en) 2021-07-15
WO2017178823A1 (en) 2017-10-19
AU2017248718A1 (en) 2018-10-25

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