GB2549298B - Super-resolution imaging - Google Patents

Super-resolution imaging Download PDF

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Publication number
GB2549298B
GB2549298B GB1606268.9A GB201606268A GB2549298B GB 2549298 B GB2549298 B GB 2549298B GB 201606268 A GB201606268 A GB 201606268A GB 2549298 B GB2549298 B GB 2549298B
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GB
United Kingdom
Prior art keywords
super
resolution imaging
resolution
imaging
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
GB1606268.9A
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English (en)
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GB2549298A (en
Inventor
Singh Ahluwalia Balpreet
Schüttpelz Mark
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universitetet I Tromso UIT
Original Assignee
Universitetet I Tromso UIT
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to GB1606268.9A priority Critical patent/GB2549298B/en
Application filed by Universitetet I Tromso UIT filed Critical Universitetet I Tromso UIT
Priority to EP17718592.3A priority patent/EP3443401B1/en
Priority to JP2019505295A priority patent/JP6997928B2/ja
Priority to AU2017248718A priority patent/AU2017248718B2/en
Priority to CN201780028957.9A priority patent/CN109313330B/zh
Priority to ES17718592T priority patent/ES2929874T3/es
Priority to PCT/GB2017/051034 priority patent/WO2017178823A1/en
Priority to CA3020529A priority patent/CA3020529C/en
Priority to US16/092,724 priority patent/US10983326B2/en
Priority to DK17718592.3T priority patent/DK3443401T3/da
Priority to KR1020187032632A priority patent/KR102409738B1/ko
Publication of GB2549298A publication Critical patent/GB2549298A/en
Application granted granted Critical
Publication of GB2549298B publication Critical patent/GB2549298B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/56—Optics using evanescent waves, i.e. inhomogeneous waves
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/02—Optical fibres with cladding with or without a coating
    • G02B6/02004—Optical fibres with cladding with or without a coating characterised by the core effective area or mode field radius
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/02—Optical fibres with cladding with or without a coating
    • G02B6/02042—Multicore optical fibres
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/10—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type
    • G02B6/12—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type of the integrated circuit kind
    • G02B6/122—Basic optical elements, e.g. light-guiding paths
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T3/00—Geometric image transformations in the plane of the image
    • G06T3/40—Scaling of whole images or parts thereof, e.g. expanding or contracting
    • G06T3/4053—Scaling of whole images or parts thereof, e.g. expanding or contracting based on super-resolution, i.e. the output image resolution being higher than the sensor resolution
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/10—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type
    • G02B6/12—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type of the integrated circuit kind
    • G02B2006/12083—Constructional arrangements
    • G02B2006/12102—Lens

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Health & Medical Sciences (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Theoretical Computer Science (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Optical Measuring Cells (AREA)
GB1606268.9A 2016-04-12 2016-04-12 Super-resolution imaging Active GB2549298B (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
GB1606268.9A GB2549298B (en) 2016-04-12 2016-04-12 Super-resolution imaging
US16/092,724 US10983326B2 (en) 2016-04-12 2017-04-12 Super-resolution imaging
AU2017248718A AU2017248718B2 (en) 2016-04-12 2017-04-12 Super-resolution imaging
CN201780028957.9A CN109313330B (zh) 2016-04-12 2017-04-12 执行对样本的超分辨率荧光显微技术成像的装置和方法
ES17718592T ES2929874T3 (es) 2016-04-12 2017-04-12 Formación de imágenes de superresolución
PCT/GB2017/051034 WO2017178823A1 (en) 2016-04-12 2017-04-12 Super-resolution imaging
EP17718592.3A EP3443401B1 (en) 2016-04-12 2017-04-12 Super-resolution imaging
JP2019505295A JP6997928B2 (ja) 2016-04-12 2017-04-12 超解像撮像
DK17718592.3T DK3443401T3 (da) 2016-04-12 2017-04-12 Superopløsningsafbildning
KR1020187032632A KR102409738B1 (ko) 2016-04-12 2017-04-12 슈퍼-해상도 이미징
CA3020529A CA3020529C (en) 2016-04-12 2017-04-12 Super-resolution imaging

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1606268.9A GB2549298B (en) 2016-04-12 2016-04-12 Super-resolution imaging

Publications (2)

Publication Number Publication Date
GB2549298A GB2549298A (en) 2017-10-18
GB2549298B true GB2549298B (en) 2022-02-02

Family

ID=58579213

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1606268.9A Active GB2549298B (en) 2016-04-12 2016-04-12 Super-resolution imaging

Country Status (11)

Country Link
US (1) US10983326B2 (https=)
EP (1) EP3443401B1 (https=)
JP (1) JP6997928B2 (https=)
KR (1) KR102409738B1 (https=)
CN (1) CN109313330B (https=)
AU (1) AU2017248718B2 (https=)
CA (1) CA3020529C (https=)
DK (1) DK3443401T3 (https=)
ES (1) ES2929874T3 (https=)
GB (1) GB2549298B (https=)
WO (1) WO2017178823A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
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EP3839483B1 (en) * 2019-12-19 2023-07-26 Imec VZW An illuminator, an imaging apparatus and a system
AU2021207899A1 (en) 2020-01-14 2022-09-08 Quantum-Si Incorporated Waveguide excitation uniformity
CN111722392B (zh) * 2020-07-22 2021-06-08 南京大学 一种基于超构透镜阵列的大视场集成显微成像装置
KR102619825B1 (ko) 2020-10-20 2024-01-03 한양대학교 산학협력단 초고해상도 형광 현미경용 이미징 버퍼 용액 및 이를 이용한 초고해상도 형광 현미경 이미징 방법
KR102654655B1 (ko) 2020-10-20 2024-04-05 한양대학교 산학협력단 초고해상도 형광 현미경 이미징 방법 및 초고해상도 형광 현미경 이미징 장치
CN113031243B (zh) * 2021-03-26 2021-08-31 杭州辰景光电科技有限公司 一种基于波导片的反射式片上数字全息显微装置
DE102021131952A1 (de) * 2021-12-03 2023-06-07 Interherence GmbH Optisches Modul
US12536611B2 (en) * 2021-12-08 2026-01-27 Bar Ilan University Method and system for super-resolved imaging
EP4220269A1 (en) * 2022-01-27 2023-08-02 Abbelight Light imaging device
KR102742598B1 (ko) * 2022-02-10 2024-12-16 한양대학교 산학협력단 형광현미경 또는 초고해상도 형광현미경을 이용한 용매의 수소 결합 센싱 방법
CN116297377B (zh) * 2023-05-22 2023-07-28 深圳高性能医疗器械国家研究院有限公司 单分子荧光检测芯片及其制备方法、单分子荧光检测仪

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DE4426968A1 (de) * 1994-07-29 1996-02-01 Bosch Gmbh Robert Lichtleiter, vorzugsweise zur seitlichen Beleuchtung eines Objektes
US20120092477A1 (en) * 2010-10-18 2012-04-19 Olympus America Inc. Wide field microscopic imaging system and method
CN102928384A (zh) * 2012-10-24 2013-02-13 浙江大学 基于微波导的超分辨显微成像方法和装置
EP2801854A1 (en) * 2013-05-10 2014-11-12 Ruprecht-Karls-Universität Heidelberg Method and apparatus for combination of localization microscopy and structured illumination microscopy
WO2016006006A1 (en) * 2014-07-09 2016-01-14 Ntp Nano Tech Projects S.R.L. Laser optical coupling for nanoparticles detection

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US20120092477A1 (en) * 2010-10-18 2012-04-19 Olympus America Inc. Wide field microscopic imaging system and method
CN102928384A (zh) * 2012-10-24 2013-02-13 浙江大学 基于微波导的超分辨显微成像方法和装置
EP2801854A1 (en) * 2013-05-10 2014-11-12 Ruprecht-Karls-Universität Heidelberg Method and apparatus for combination of localization microscopy and structured illumination microscopy
WO2016006006A1 (en) * 2014-07-09 2016-01-14 Ntp Nano Tech Projects S.R.L. Laser optical coupling for nanoparticles detection

Also Published As

Publication number Publication date
CN109313330A (zh) 2019-02-05
JP6997928B2 (ja) 2022-01-18
DK3443401T3 (da) 2022-10-24
KR20180130567A (ko) 2018-12-07
EP3443401A1 (en) 2019-02-20
CN109313330B (zh) 2021-06-15
JP2019516140A (ja) 2019-06-13
CA3020529C (en) 2023-10-17
ES2929874T3 (es) 2022-12-02
GB2549298A (en) 2017-10-18
EP3443401B1 (en) 2022-07-20
KR102409738B1 (ko) 2022-06-15
US10983326B2 (en) 2021-04-20
US20190129161A1 (en) 2019-05-02
CA3020529A1 (en) 2017-10-19
AU2017248718B2 (en) 2021-07-15
WO2017178823A1 (en) 2017-10-19
AU2017248718A1 (en) 2018-10-25

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