KR102406692B1 - Composition for promoting hair growth comprising germinated gemmule of bean - Google Patents

Abstract

The present invention relates to a composition for promoting hair growth comprising a soybean germination germ extract as an active ingredient, and more specifically, a cosmetic composition for promoting hair growth or improving hair loss comprising a bean germination germ extract as an active ingredient; A pharmaceutical composition for promoting hair growth, preventing and treating hair loss; A quasi-drug composition for promoting hair growth or alleviating hair loss; Health functional food composition for promoting hair growth, preventing or improving hair loss; And it relates to a method for promoting hair growth or improving hair loss comprising administering to an individual other than a human having symptoms of hair loss.
The composition comprising the soybean germ germ extract of the present invention can be applied and used in cosmetics, pharmaceuticals, etc. for promoting hair growth, alleviating and treating hair loss.

Description

Composition for promoting hair growth comprising germinated soybean germ extract as an active ingredient {Composition for promoting hair growth comprising germinated gemmule of bean}

The present invention relates to a composition for promoting hair growth comprising an extract of soybean germination as an active ingredient, and more particularly, a cosmetic composition for promoting hair growth or improving hair loss comprising an extract of germination bean germ as an active ingredient; A pharmaceutical composition for promoting hair growth, preventing and treating hair loss; A quasi-drug composition for promoting hair growth or alleviating hair loss; Health functional food composition for promoting hair growth, preventing or improving hair loss; And it relates to a method for promoting hair growth or improving hair loss, comprising administering to a subject other than a human having symptoms of hair loss.

Currently, interest in hair loss suppression is gradually increasing. The world's hair loss population is steadily increasing, and minoxidil is widely used as a medicine to induce the effect of wool. However, in the case of minoxidil, since the principle of inducing wool is vasodilatation, it induces dilatation of arteries, but maintains veins to induce a difference in blood pressure overall, and it is difficult to use in combination with antihypertensive drugs. In addition, it is a major side effect that it is found that it induces hair in a wide area rather than a local area, leading to unexpected hirsutism in female patients. Therefore, more than half of hair loss patients prefer hair loss relief products to avoid the side effects of current medicines.

On the other hand, dermal papilla cells (DPCs) are cells that exist at the base of the hair follicle, and are responsible for hair growth and hair cycle regulation by supplying oxygen and nutrients to the cells constituting the hair follicle. Therefore, when the proliferation of dermal papilla cells is promoted, hair becomes healthy, hair growth is promoted, and hair loss can be prevented. Because hair growth proceeds as the epithelial cells surrounding the dermal papilla divide to form a hair shaft, the dermal papilla cells play an important role in regulating the division of epithelial cells. Also, in androgenetic alopecia, the site where male hormones act on hair follicles is also the dermal papilla, and dermal papilla cells play a very important role in hair growth.

According to a study conducted by Jahoda (Jahoda CA, et al., Induction of hair growth by implantation of cultured dermal papilla cells., Nature, 311, pp.560-562, 1984) et al. cell), hair growth temporarily stopped, and then the cells moved from the dermal root to form dermal papilla cells again, and hair growth continued again. It is reported that hair growth continues as dermal papilla cells are formed and matrix cells are formed from the integumentary root sheath, but hair growth stops when more than 1/3 of the lower part of the hair follicle is removed. In addition, after removing more than 1/3 of the lower part of the hair follicle, it was reported that hair growth continued when the isolated dermal papilla cells were transplanted again. said.

Currently, hair loss relief products that have fewer side effects and are less risky for women and patients taking blood pressure medications are on the market. Typically, biotin, nicotinic acid amide, pyrimidine zinc solution series, or vegetable complexes, etc. (Korea Patent 10-1127331; Korea Patent Publication 10-2019-0125775) are supplied to help alleviate hair loss by supplying hair components. . Up to now, medicinal herbs centered on the supply of nutrients are remarkably lacking in efficacy compared to minoxidil.

Under the above background, the present inventors have made diligent efforts to effectively prevent / treat hair loss and develop a method for improving hair growth. The invention was completed.

SUMMARY OF THE INVENTION It is an object of the present invention to provide a cosmetic composition for promoting hair growth or improving hair loss comprising a bean germ germ extract as an active ingredient.

Another object of the present invention is to provide a pharmaceutical composition for promoting hair growth, preventing and treating hair loss, comprising a bean germ embryo extract as an active ingredient.

Another object of the present invention is to provide a quasi-drug composition for promoting hair growth or alleviating hair loss comprising a bean germ embryo extract as an active ingredient.

Another object of the present invention is to provide a health functional food composition for promoting hair growth, preventing or improving hair loss, comprising a bean germ embryo extract as an active ingredient.

Another object of the present invention is to provide a method for promoting hair growth or improving hair loss, comprising administering the soybean germ embryo extract as an active ingredient to individuals other than humans with hair loss symptoms.

As one aspect for achieving the above object, the present invention provides a cosmetic composition for promoting hair growth or improving hair loss, comprising a bean germ germ extract as an active ingredient.

As used herein, the term “germinated soybean” refers to a germinated embryo obtained by germinating an embryo isolated from soybeans. The method for producing a soybean germinated embryo has been known by the previous studies of the present inventors (Korea Patent Publication No. 2013-0057173), and specifically, the soybean germinated embryo may be obtained by germinating an embryo separated from soybean, However, the present invention is not limited thereto. The effect of improving hair growth or hair loss of the soybean germination extract was not known at all until now, and was first identified by the present inventors.

The term "extract" of the present invention refers to an extract obtained by extracting soybean germination, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a prepared or purified product of the extract, or a mixture thereof, such as an extract It includes extracts of all formulations that can be formed by themselves and using extracts.

In the present invention, the method for extracting the soybean germinated embryo is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include hot water extraction, ultrasonic extraction, filtration, and reflux extraction, and these may be performed alone or in combination of two or more methods.

In the present invention, the type of extraction solvent used to extract the soybean germinated embryo is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water, alcohol, or a mixed solvent thereof, and these may be used alone or in combination of one or more. When alcohol is used as the solvent, alcohol having 1 to 4 carbon atoms may be specifically used.

The soybean germination embryo content of the soybean germination embryo extract may be 1 to 100% by weight based on the total weight.

As used herein, the term "fraction" refers to a result obtained by performing fractionation in order to separate a specific component or a specific component group from a mixture including various components.

The fractionation method for obtaining the fraction in the present invention is not particularly limited, and may be performed according to a method commonly used in the art. Non-limiting examples of the fractionation method include a method of obtaining a fraction from the extract by treating the extract obtained by extracting the germinated soybean embryo of the present invention with a predetermined solvent.

In the present invention, the type of the fractionation solvent used to obtain the fraction is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the fractionation solvent include polar solvents such as water and alcohol; and non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of one or more. When alcohol is used in the fractionation solvent, alcohol having 1 to 4 carbon atoms may be specifically used.

As used herein, the term “promoting hair growth” refers to promoting hair growth and ultimately increasing the proportion of hair growth phase in total hair. Accordingly, the term suppresses hair loss caused by a decrease in the specific gravity of hair growth in the growth phase, and may have the same meaning as “improving hair growth”.

As used herein, the term “improving hair loss” refers to the action of preventing hair loss by weakening the degenerative phase of the hair follicle and inducing the growth phase. Accordingly, the term suppresses hair loss caused by a decrease in the proportion of hair in the growth phase, and may have the same meaning as “hair loss mitigation”, “hair loss prevention” and “hair loss treatment”.

As used herein, the term “prevention” refers to any action in which the composition of the present invention suppresses or delays hair loss by weakening the degenerative phase of hair follicles and inducing the growth phase.

In the present invention, the term “treatment” refers to any action in which the composition of the present invention suppresses or delays hair loss by weakening the degenerative phase of hair follicles and inducing the growth phase.

The term “cosmetic composition” of the present invention can be prepared in the form of general emulsified formulations and solubilized formulations. The emulsified formulation includes nutrient lotion, cream, essence, and the like, and the solubilized formulation includes softening lotion and the like. The cosmetic composition is a formulation selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, ampoules, powder foundation, emulsion foundation, wax foundation, and spray may be manufactured, but is not limited thereto. Specifically, it is manufactured in the form of hypoallergenic cosmetic skin protectant, flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, serum, cleansing cream, cleansing foam, cleansing water, pack, cream, essence, tray or powder. can be

In addition, the cosmetic composition may further include at least one cosmetically acceptable carrier to be formulated in general skin cosmetics, and as common ingredients, for example, oil, water, surfactant, humectant, lower alcohol, thickener, A chelating agent, a colorant, a preservative, a fragrance, etc. may be appropriately mixed, but the present invention is not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition varies depending on the formulation.

When the formulation of the cosmetic composition is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide as a carrier component or mixtures thereof may be used.

When the formulation of the cosmetic composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or a mixture thereof may be used as a carrier component, and in particular, in the case of a spray, additionally propellants such as chlorofluorohydrocarbons, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol. , 1,3-butylglycol oil may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol fatty ester, fatty acid ester of polyethylene glycol or sorbitan may be used. can

When the formulation of the cosmetic composition is a suspension, a liquid diluent such as water, ethanol or propylene glycol as a carrier component, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the cosmetic composition is soap, alkali metal salt of fatty acid, fatty acid hemiester salt, fatty acid protein hydrolyzate, isethionate, lanolin derivative, aliphatic alcohol, vegetable oil, glycerol, sugar, etc. are used as carrier components can be

In one embodiment of the present inventors, by treating the dermal papilla cells with a composition containing soybean germination embryos to check the expression changes of BMP4, Wnt5a, and FGF7, which regulate the cycle of dermal papilla cells, treatment in a mouse model promotes significant hair growth than minoxidil By confirming the efficacy, the present invention was completed.

The term, “BMP protein” is known to be highly expressed during the early stage of hair follicle development and the transition to the growth phase (telogen-to-anagen transition), and to decrease after the development of the anagen phase.

As used herein, the term “Wnt protein” is known to play an important role in embryonic development, morphogenesis, body patterning, axis formation, proliferation, cell motility, and the like. is known

The term, “Fibroblast growth factor (FGF) protein” is a fibroblast growth factor, which is known as a protein that promotes the proliferation of various types of cells, and is also known to promote the proliferation of hair matrix cells. .

In addition, the BMP, Wnt, and FGF proteins are known to regulate the mother cycle through interaction with each other.

The term, “dermal papilla cell (DPC)” is a cell present at the base of the hair follicle, and is known to be responsible for hair growth and hair cycle regulation by supplying oxygen and nutrients to the cells constituting the hair follicle. In addition, it is known that when the proliferation of dermal papilla cells is promoted, hair becomes healthy, hair growth is promoted, and hair loss can be prevented.

The term, “hair cycle” refers to the cycle of hair, specifically, anagen to grow hair, catagen, which is a time when growth ends and the hair bulb shrinks, and dermal papilla to stop activity and restore hair. It refers to the telogen, which is the period of staying on the scalp, and the active period, when the dermal papilla starts to act or generates new hair to hair out old hair.

Another aspect of the present invention provides a pharmaceutical composition for promoting hair growth, preventing and treating hair loss, comprising the soybean germination embryo extract as an active ingredient.

The terms "bean germination embryo", "hair loss prevention", and "hair loss treatment" of the present invention are the same as described above.

In the present invention, the pharmaceutical composition may further include a pharmaceutically acceptable carrier.

In the present invention, the term “pharmaceutically acceptable carrier” refers to a carrier or diluent that does not stimulate the organism and does not inhibit the preventive or therapeutic activity and properties of the skin hyperpigmentation disease symptoms of the composition of the present invention. Examples of acceptable pharmaceutical carriers for compositions formulated as liquid solutions are sterile and biocompatible, and include saline, sterile water, Ringer's solution, buffered saline, albumin injection, dextrose solution, maltodextrin solution, glycerol, ethanol. And one or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers, and bacteriostats may be added as needed.

In addition, the pharmaceutically acceptable carrier of the present invention may include a non-natural carrier.

The pharmaceutical composition of the present invention may be administered in a pharmaceutically effective amount, and the term, “pharmaceutically effective amount” is sufficient to treat or prevent a disease at a reasonable benefit/risk ratio applicable to medical treatment or prevention. means the amount, and the effective dose level is the severity of the disease, the drug activity, the patient's age, weight, health, sex, the patient's sensitivity to the drug, the administration time of the composition of the present invention used, the route of administration and the rate of excretion Treatment period , may be determined according to factors including drugs used in combination with or concurrently with the composition of the present invention and other factors well known in the medical field. The pharmaceutical composition of the present invention may be administered alone or in combination with a known therapeutic agent for pterygium. Taking all of the above factors into consideration, it is important to administer an amount that can obtain the maximum effect with a minimum amount without side effects.

Another aspect of the present invention provides a quasi-drug composition for promoting hair growth or alleviating hair loss, comprising a bean germ germ extract as an active ingredient.

The terms “bean germination”, “extract”, “promoting hair growth”, and “relieving hair loss” of the present invention are the same as described above.

In the present invention, the term “quasi-drugs” refers to items with a milder action than pharmaceuticals among items used for the purpose of diagnosing, treating, improving, alleviating, treating or preventing diseases of humans or animals. Quasi-drugs exclude products used for pharmaceutical purposes, and include products used for the treatment or prevention of diseases in humans and animals, and products with minor or no direct action on the human body.

The quasi-drug composition of the present invention may be prepared in a form selected from the group consisting of body cleanser, foam, soap, mask, ointment, cream, lotion, essence and spray, but is not limited thereto.

When the composition containing the soybean germination germ extract of the present invention is used as a quasi-drug additive, the composition containing the soybean germination germ extract of the present invention can be added as it is or used together with other quasi-drugs or quasi-drug ingredients, can be used

Another aspect of the present invention provides a health functional food composition for promoting hair growth, preventing or improving hair loss, comprising the soybean germination embryo extract as an active ingredient.

The terms “bean germination”, “extract”, “promotion of hair growth”, “prevention of hair loss”, and “improvement” of the present invention are the same as described above.

In the present invention, the term “health functional food” refers to food manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills, etc. using raw materials or ingredients useful for the human body.

Here, the term “functionality” refers to obtaining useful effects for health purposes such as regulating nutrients or physiological effects on the structure and function of the human body. The health functional food of the present invention can be prepared by a method commonly used in the art, and during the manufacture, it can be prepared by adding raw materials and components commonly added in the art. In addition, the dosage form of the health functional food may be manufactured without limitation as long as it is a dosage form recognized as a health functional food.

The health functional food of the present invention can be manufactured in various types of dosage forms, and unlike general drugs, it has the advantage that there are no side effects that may occur when taking the drug for a long period of time by using food as a raw material, and it is excellent in portability, and the present invention health functional food can be ingested as a supplement to enhance the effect of promoting wool and alleviating hair loss.

The health functional food of the present invention may contain various flavoring agents or natural carbohydrates as additional ingredients. The above-mentioned natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. As the sweetener, natural sweeteners such as taumatine and stevia extract or synthetic sweeteners such as saccharin and aspartame may be used.

In addition to the above, the health functional food of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, Carbonating agents used in carbonated beverages, etc. may be contained. These components may be used independently or in combination. Although the proportion of these additives is not very important, it is generally selected in the range of 1 to 100 parts by weight per 100 parts by weight of the health food of the present invention, but is not particularly limited thereto.

Another aspect of the present invention provides a method for promoting hair growth or improving hair loss, comprising administering to an individual other than a human having symptoms of hair loss comprising a bean germ embryo extract as an active ingredient.

In the method for promoting hair and alleviating hair loss of the present invention, the bean germ embryo extract and hair growth promotion and hair loss improvement are as described above.

Administration of the present invention may be a method, comprising application to the skin.

The composition comprising the germinated soybean embryo of the present invention may be administered to an individual in need thereof in an effective amount. The level of the effective dose can be determined by those skilled in the art based on common common sense to the extent that the desired effect of promoting hair growth and improving hair loss appears.

The composition comprising the soybean germination germ extract of the present invention can be applied and used in cosmetics, pharmaceuticals, etc. for promoting hair growth, alleviating and treating hair loss.

1 is a graph showing the relative expression level of BMP4 according to the treatment with soybean germinated embryo extract.
2 is a graph showing the relative expression level of Wnt5a according to the treatment with soybean germinated embryo extract.
3 is a graph showing the relative expression level of FGF7 according to the treatment with soybean germinated embryo extract.
4 is a photograph showing the effect of improving hair growth by treating the soybean germination embryo extract in mice.

Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

Example 1: Preparation of soybean germination extract

In order to prepare a soybean germinated embryo extract, the soybean germinated embryo prepared according to the previous study of the present inventor was used. Specifically, soybean embryos were immersed in running water to germinate, and when about 24 hours had elapsed, soybean germinated embryos were harvested and freeze-dried, which was then pulverized to obtain soybean germination powder. The obtained soybean germinated germ powder was extracted by adding fermented alcohol, filtered to obtain a liquid component, and the liquid component was freeze-dried to obtain a soybean germinated germ extract (GSGE).

Example 2: Changes in expression of factors regulating the cycle of dermal papilla cells

By observing the change in the expression of factors involved in the hair cycle regulation of dermal papilla cells that control the development and growth of hair, the hair growth promoting effect of the soybean germination embryo (GESE) prepared in Example 1 was confirmed.

More specifically, after culturing in the form of a 3D spheroid using human dermal papilla cells, treatment with 100 μg/mL soybean germ extract, 1 day later, mRNA expression of BMP4, Wnt5a, and FGF7 was Confirmed. In addition, the untreated group and the germinated soybean extract were compared with the positive control group.

As a result, as can be seen in FIG. 1 , it was confirmed that the expression of BMP4 increased about 3 times compared to the untreated group, which was significantly higher than that of the germinated soybean extract treated group.

In addition, as can be seen in FIG. 2 , it was confirmed that the expression of Wnt5a increased about 1.5 times compared to the untreated group, which was significantly higher than that of the germinated soybean extract treated group.

In addition, as can be seen in FIG. 3 , it was confirmed that the expression of FGF7 was decreased by about 4.5 times compared to the untreated group, which was significantly lower than that of the germinated soybean extract treated group.

Through this, it was confirmed that the effect of changing the hair cycle control of the germinated soybean extract was significantly higher than that of the germinated soybean extract treatment.

Example 3: Efficacy of hair growth improvement in animal experiments

In order to test the hair growth improvement efficacy of the soybean germinated embryo extract, an experiment was performed on mice that were synchronized with the hair cycle by shaving with a razor.

More specifically, male C57BL/6 mice were acclimatized to growth in a breeding facility for 7 days (1 week), and the condition of the experimental animals was optimally maintained in each cage until the experiment was started. Thereafter, the body hair on the back of the mouse was removed with a razor blade, and after alleviating red spots and inflammation, the surrounding fine hairs were completely removed using a hair removal cream. After that, 5 mg/mL of germinated soybean extract and 5 mg/mL of germinated soybean extract were treated, and the untreated group, the positive control group treated with minoxidil (3% minoxidil), and the solvent-treated group (50% propylene glycol/ 25% ethanol) to evaluate the effect of improving hair growth.

As a result, as can be seen in FIG. 4 , in the mouse model in which the mother cycle was synchronized, the germinated soybean extract treated group and the soybean germinated germ extract treated group compared to the untreated group, the minoxidil treated group, and the solvent treated group throughout the skin. It was confirmed that hair growth was uniformly promoted, and it was confirmed through the naked eye that the hair quality was improved due to increased gloss.

Through this, it was confirmed that the soybean germination germ extract had significantly superior hair growth promoting effect compared to the minoxidil-treated group.

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention, rather than the above detailed description, all changes or modifications derived from the meaning and scope of the following claims and their equivalents.

Claims (9)

A cosmetic composition for promoting hair growth or improving hair loss, comprising bean germ germ extract as an active ingredient.
The cosmetic composition according to claim 1, wherein the soybean germination extract is obtained by extraction with water, ethanol, or a mixed solvent thereof.
The cosmetic composition according to claim 1, wherein the soybean germinated embryo is obtained by germinating an embryo separated from soybean.
The cosmetic composition of claim 1, wherein the composition further comprises a cosmetically acceptable carrier, excipient or diluent.
According to claim 1, wherein the hair growth promotion or hair loss improvement is due to the hair cycle regulation of dermal papilla cells, the cosmetic composition.
A pharmaceutical composition for promoting hair growth, preventing or treating hair loss, comprising a bean germ germ extract as an active ingredient.
A quasi-drug composition for promoting hair growth or alleviating hair loss, comprising bean germ germ extract as an active ingredient.
A health functional food composition for promoting hair growth, preventing or improving hair loss, comprising the soybean germination embryo extract as an active ingredient.
A method for promoting hair growth or improving hair loss, comprising administering to an individual other than a human having symptoms of hair loss, comprising the soybean germination embryo extract as an active ingredient.

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