KR102386693B1 - Antiviral disinfectant containing plum sugar extract as an active ingredient - Google Patents

Abstract

The present invention relates to an antiviral disinfectant containing a Japanese apricot sugar extract as an active ingredient. The disinfectant additionally includes a Firmiana simplex extract, a Robinia pseudoacacia extract, a coltsfoot extract, a Chinese cabbage extract, and an asparagus extract, and is added with citron essential oil, which is a natural substance, as a fragrance, and thus it is confirmed that the antiviral disinfectant is harmless to a human body and has an excellent killing effect of various viruses. Accordingly, a natural antiviral disinfectant can be provided by using the disinfectant as a gel-type hand disinfectant or a spray-type indoor disinfectant.

Description

Antiviral disinfectant containing plum sugar extract as an active ingredient}

The present invention relates to an antiviral disinfectant containing a plum sugar extract as an active ingredient.

The ultimate goal of both public health and sanitation is disease prevention. Diseases are affected by pathogens, hosts, and the environment. Among them, pathogens, which are external factors, can be prevented by methods such as disinfection (Korean Society of Veterinary Public Health, Veterinary Public Health Education Council edition, Veterinary Public Health, Moon Undang) , pp.1-2, 1996). Humans have long been using natural products to remove pathogens or viruses.

Plum is a substance that has been used since ancient times for the purpose of preventing and treating food poisoning and infectious diseases. Even now, a lot of research is being done for the purpose of food preservation, and in particular, it contains a lot of citric acid and malic acid, so its sterilizing power is widely known. Plum extract is known to be effective against all gram-negative and positive bacteria such as E. coli (O157:H77), Vibrio parahaemolyticus , Salmonella typhimurium , etc. Women's University master's thesis, 1986), the inhibitory effect on the virus is still under study Influenza is a kind of respiratory disease virus that spreads widely to not only humans but also livestock such as pigs, chickens, ducks or horses, as well as various kinds of animals Corona virus is mainly susceptible to infecting children and young animals (Elsevier, 2008, 554; Advances in Virus Research, 2006, 66, 193-232) On the other hand, CCV is mostly mild or asymptomatic. In many cases, it can cause severe gastroenteritis.

While the present inventors were conducting various studies on virus-related disinfectants, which have become a problem in recent years, using plums, the disinfectant composition containing the sugar extract of plums as an active ingredient has an inhibitory effect on various viruses, The present invention was completed by confirming that it can be used as a spray-type disinfectant for indoor and outdoor use.

Korean Patent Laid-Open Patent No. 10-2005-0117975 (Title of the invention: Natural antioxidant composition containing Byeopodong extract and manufacturing method thereof, Applicant: Jinsoo Kim, Publication date: December 15, 2005) Republic of Korea Patent Registration No. 10-0528267 (Title of the invention: Viricide composition using natural products, Applicant: R&L Life Sciences Co., Ltd., Registration date: November 07, 2005) Republic of Korea Patent Registration No. 10-1633432 (Title of the invention: Use of acacia extract for improvement and treatment of Gamma Herpes Virus infection, Applicant: R&L Life Sciences Co., Ltd., Registration date: June 20, 2016)

An object of the present invention is to provide an antiviral disinfectant containing a plum sugar extract as an active ingredient.

The present invention relates to an antiviral disinfectant comprising a plum sugar extract, an extract of chrysanthemum paulownia extract, an acacia extract, a coltsfoot extract, a Chinese cabbage extract, and an asparagus extract.

The disinfectant is based on 100 parts by weight of extract per plum, 10 to 20 parts by weight of Byeopodong extract, 20 to 30 parts by weight of acacia extract, 3 to 5 parts by weight of coltsfoot extract, 20 to 30 parts by weight of Chinese cabbage extract, and 10 to 15 parts by weight of asparagus extract may be contained. The disinfectant may be diluted in 100 to 2000 times the weight of the disinfectant immediately before use in purified water.

The antiviral disinfectant may contain yuja essential oil. The yuja essential oil may be mixed in an amount of 0.0001 to 0.1 parts by weight based on 100 parts by weight of the extract per plum.

For each extract, each raw material sample is prepared, and the raw material sample can be extracted using water, C1-C4 alcohol, or a mixed solution thereof as a solvent. As the solvent, it is best to preferably use water.

In addition, the disinfectant is characterized in that it has an antibacterial effect.

The disinfectant may be in a gel-type or spray-type formulation.

Hereinafter, the present invention will be described in detail.

The plum sugar extract used in the present invention may be a liquid obtained by removing impurities from the osmotic extraction by leaving a mixture of sugar mixed with plum as it is.

Based on 100 parts by weight of plum, a mixture of 50 to 200 parts by weight of sugar is left as it is for 3 to 6 months, and may be a liquid obtained by removing impurities from the osmotic extraction. The extraction temperature of the plum sugar extract is preferably 4 ~ 37 ℃, more preferably 15 ~ 30 ℃. As the saccharide, any sweetener may be used, but at least one selected from the group consisting of sugar, sugar powder, oligosaccharide, agave syrup, maple syrup, cactus sugar and honey may be used.

At this time, when the sugar content is less than 50 parts by weight based on 100 parts by weight of plum, fermentation does not occur and it is easy to spoil. In addition, when the sugar content exceeds 200 parts by weight based on 100 parts by weight of the plum, the juice extracted from the plum may not be mixed with the sugar due to over-addition of the sugar component. In addition, if the extraction period is less than 3 months, it is easy to decay thereafter, and if the extraction period exceeds 6 months, acetic acid fermentation or alcohol fermentation may proceed, and spoilage may proceed depending on the storage place or environment.

The Byeok Paulownia used in the present invention may contain at least one selected from branches, fruits and leaves, and woody parts may be used as acacia (acacia).

Each solvent extract in the disinfectant may be mixed with the plum sugar extract after freeze-drying, but when the extract is extracted with water, it may be mixed directly with the plum sugar extract. Preferably, the solvent extract may be in a liquid form by adding 5 to 20 parts by weight of water compared to the raw material sample, and then extracting by heating at 70 to 90° C. for 1 to 10 hours, and then removing the ingredients.

The citron essential oil used in the present invention is prepared by steam fumigation in a conventional manner using citron skin as a raw material, and more preferably, cold-pressing the raw citron skin to obtain a juice, and the juice is applied to a thin film. It may be obtained by fumigating the oil component through a distiller and condensing it. In the present invention, commercially available products may be used.

As the solvent extract to be used in the present invention, the extract of Byeokpaul-dong, acacia extract, coltsfoot extract, Chinese cabbage extract, and asparagus extract can be prepared by the following method. To this end, each raw material sample is prepared, and the raw material sample can be extracted using water, C1-C4 alcohol or a mixed solution thereof as a solvent, and the C1-C4 alcohol is methanol, ethanol, propanol, isopropanol, butanol and iso It may be selected from the group consisting of butanol.

The extraction conditions of the raw material sample may be 1 minute to 48 hours at 20 ~ 100 ℃. The above process can be repeated 1 to 4 times. As the extraction device used at this time, a conventional extraction device, an ultrasonic crushing extractor or a fractionator may be used. The solvent extract thus prepared may be dried by hot air drying, reduced pressure drying, or freeze-drying to remove the solvent. In addition, the solvent extract may be purified and used using column chromatography. The solvent extract is a known method used for separation and extraction of plant components, such as extraction with an organic solvent (alcohol, ether, acetone, etc.), distribution of hexane and water, and a method by column chromatography according to a conventional method. It can be used after fractionation or purification using a combination method. The chromatography is silica gel column chromatography (silica gel column chromatography), LH-20 column chromatography (LH-20 column chromatography), ion exchange resin chromatography (ion exchange resin chromatography), medium pressure liquid chromatography (medium pressure liquid chromatography) chromatography), thin layer chromatography (TLC), silica gel vacuum liquid chromatography, and high performance liquid chromatography may be selected.

The disinfectant of the present invention can be prepared in a gel formulation or a spray type spray formulation. When the disinfectant is prepared as a hand sanitizer, it may be provided by diluting the stock solution as it is, and various excipients may be included. To this end, a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extract may be additionally included. Other ingredients that may be added include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, colorants, fragrances, A blood circulation promoter, a cooling agent, a restrictive agent, purified water, etc. are mentioned. When the disinfectant is a spray or spray, it may additionally contain a propellant such as chlorofluorohydrocarbon, propane/butane or dimethyl ether.

The present invention relates to an antiviral disinfectant containing a plum sugar extract as an active ingredient, wherein the disinfectant is citron essential, which is a natural substance as a flavoring agent, further added with a byeok paulownia extract, acacia extract, coltsfoot extract, Chinese cabbage extract and asparagus extract. By adding the oil, it is confirmed that the effect of killing various viruses is excellent while being harmless to the human body. Accordingly, by using the disinfectant as a gel-type hand sanitizer or a spray-type indoor disinfectant, a natural antiviral disinfectant can be provided.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided to fully convey the spirit of the present invention to those skilled in the art so that this disclosure will be thorough and complete.

<Examples 1 to 6. Preparation of antiviral disinfectant>

A mixture of plums and sugar in equal weight was left at a cool and shaded room temperature of 25° C. for 3 months. When the juice was sufficiently extracted from the plums, the ingredients were removed and the liquid was collected to obtain a plum sugar extract.

A sample was prepared by mixing eggplants, fruits, and leaves in equal weights for Byeokpaul-dong, and acacia (acacia) prepared the woody parts of branches and stems. Byeochodong, acacia, coltsfoot (fruit), Chinese cabbage, and asparagus were prepared raw. Yuzu essential oil was purchased commercially. Each raw material from Byeokgongdong to asparagus was extracted by heating at 80°C for 5 hours by adding 10kg of water per 1kg.

Next, an antiviral disinfectant composition was prepared by mixing as shown in Table 1 below based on 100 g of the plum sugar extract.

Condition plum
Party
extract
(g) Byeo-dong
extract
(g) acacia extract
(g) coltsfoot
extract
(g) cabbage
extract
(g) Aspa
lagus
extract
(g) Citron
essential
oil
(g) sum
(g) Example 1 100 15 25 4 25 13 0 182 Example 2 100 10 29 3 27 13 0 182 Example 3 100 17 20 5 30 10 0 182 Example 4 100 20 22 5 20 15 0 182 Example 5 100 13 30 4 20 15 0 182 Example 6 100 15 25 4 25 12.99 0.01 182

<Comparative Examples 1 to 5. Preparation of antiviral disinfectant under comparative conditions>

Comparative compositions for antiviral disinfectants were prepared under the conditions of Table 2. Each raw material and manufacturing method were the same as in Example 1.

Condition plum
Party
extract
(g) Byeok Paulownia Extract
(g) acacia extract
(g) butterbur extract
(g) Chinese cabbage extract
(g) Aspa
lagus
extract
(g) Citron
essential
oil
(g) sum
(g) Comparative Example 1 182 0 0 0 0 0 0 182 Comparative Example 2 0 100 60 10 5 7 0 182 Comparative Example 3 100 50 32 0 0 0 0 182 Comparative Example 4 100 0 0 40 20 22 0 182 Comparative Example 5 0 100 82 0 0 0 0 182

Part of the disinfectant composition prepared in Table 1 was diluted in purified water by 100 times by weight and put in a sprayer to prepare an indoor disinfectant, and part of each of the above-mentioned alcohol gels containing 70% by weight of ethanol and fresh aloe vera juice as a thickener It was prepared as a hand sanitizer so that the disinfectant was 1% by weight.

<Comparative Example 6. Preparation of antiviral disinfectant using plum juice>

The plum used in the preparation of the plum sugar extract used in Example 1 was taken by the same weight and compressed to obtain a juice. Sugar was added in the same weight as the juice and boiled slightly to dissolve the sugar. While using the so-prepared plum juice instead of the plum sugar extract in Example 1, it was mixed with other extracts of Example 1.

<Comparative Example 6. Preparation of antiviral disinfectant using plum extract>

The plum used in the preparation of the plum sugar extract used in Example 1 was taken at the same weight and prepared in the same manner as for preparing extracts of other raw materials, and the water was evaporated to the same volume as the plum sugar extract, which was obtained in Example While using instead of the plum sugar extract in 1, it was mixed with the other extracts of Example 1.

<Experimental Example 1. Confirmation of antiviral efficacy i>

The antiviral activity of the sample was measured according to ASTM E1052-20, Human respiratory syncytial virus (RSV, ATCC VR-26), Rotavirus A (RVA, ATCC VR-2018) and Human coronavirus (CoV, ATCC VR-740) The sample solution was mixed with the virus culture solution and contacted for 20 minutes, and then the virus activity was confirmed. Virus activity was measured by 50% tissue culture infectious dose assay [tissue culture 50% infectious dose assay (log ₁₀ TCID ₅₀ )] after infecting the host cells with the virus. In this experiment, each sample in the liquid phase was freeze-dried and reconstituted in water to confirm the concentration accurately. Each sample used in the experiment was used as a standard of 25 μg/ml.

As host cells, Hep-2 (KCLB 10023), CV-1 (KCLB 10070), and MRC-5 (ATCC CCL-171) were used. For cell culture medium, 10% of FBS was added to EMEM, RSV was used as a virus culture medium, CoV was obtained by adding 2% of FBS to EMEM, and RVA was 2㎍/㎖ Trypsin in EMEM. To determine the viral titer, Hep-2 cells were infected with RSV, CV-1 cells were infected with RVA, and MRC-5 cells were infected with CoV. In addition, it was confirmed that each virus had no cytotoxicity up to 10 ^-3 and no cytotoxicity, so it was confirmed that it was a suitable condition for a titer measurement experiment.

Condition log ₁₀ TCID ₅₀ RSV CoV RVA untreated group 7.5 5.5 6.5 Example 1 3.5 2.5 2.5 Example 2 3.5 2.5 2.5 Example 3 3.0 2.0 2.0 Example 4 4.5 2.5 2.0 Example 5 3.5 2.5 3.0 Example 6 2.5 1.5 1.5 Comparative Example 1 6.5 5.0 4.5 Comparative Example 2 7.0 5.0 4.0 Comparative Example 3 6.5 5.5 5.0 Comparative Example 4 6.5 4.5 4.5 Comparative Example 5 5.5 4.5 4.5 Comparative Example 6 5.0 4.5 4.0 Comparative Example 7 5.0 4.0 4.5

As a result of the confirmation, the compositions of Examples 1 to 7 showed very high virus titers against Human respiratory syncytial virus (RSV), Rotavirus A (RVA) and Human coronavirus (CoV). Suitable for use as a hand sanitizer or disinfectant for indoor and outdoor spray can be checked.

<Experimental Example 2. Confirmation of antiviral efficacy ii>

In order to additionally confirm antiviral activity, influenza virus type A (H1N1) and seasonal influenza virus type A (H3N2) were infected with MDCK (Mardin Darby Canine Kidney) cell lines, and then, Examples 1 to 6, Comparative Examples 1 to 7 After freeze-drying the disinfectant composition, it was prepared as an aqueous solution again, but the antiviral activity was analyzed by treatment in a concentration range of 2 to 10 mg/ml. The MDCK cell line used in the experiment was routinely cultured in Eagle's minimum essential medium (MEM) containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin solution (Gibco). prepared

1 × 10 ⁴ MDCK cells per 1 ml were dispensed in a 96-well plate and cultured at 37° C. for 16 hours using a 5% CO ₂ incubator. After washing the monolayer MDCK cells twice with PBS, influenza virus type A (H1N1) and seasonal influenza virus type A (H3N2) were infected with 100 TCID ₅₀ at 37°C for 2 hours, and then uninfected viruses were washed did

Infected cells were sequentially diluted with a virus culture solution containing the compositions of Examples and Comparative Examples (0.3% Bovine serum albumin, 1% Penicillin-Streptomycin solution) and L-1-tosylamido-2-phenylethyl at 1 μg/ml In MEM culture medium containing trypsin-TPCK treated with chloromethyl ketone, it was cultured at 37° C. for 48 hours until viral CPE (cytophatic effect) appeared. The antiviral CPE lowering ability of the compositions was expressed as a value of 50% of the effective concentration of viral inhibition (EC ₅₀ ), and the value of 50% of the cytotoxic concentration (CC ₅₀ ) was determined based on the morphological modification of cells. The anti-influenza virus ability of the compositions was expressed as a selectivity index (SI) obtained by dividing a CC ₅₀ value by an EC ₅₀ value. SI (selectivity index, selectivity index) is CC ₅₀ /EC ₅₀ , and the larger the SI value, the greater the virus proliferation inhibitory effect. Table 3 shows the results of comparing the anti-influenza virus activity against H1N1 and H3N2 of the compositions in MDCK cells.

Condition CC ₅₀ (μg/ml) EC ₅₀ (μg/ml) SI H1N1 H3N2 H1N1 H3N2 H1N1 H3N2 Example 1 7534 7523 14.9 46.9 505.6 160.4 Example 2 7321 7245 29.3 35.2 249.9 205.8 Example 3 7831 7325 53.2 45.2 147.2 162.1 Example 4 7190 7821 34.2 45.2 210.2 173.0 Example 5 7422 7492 36.2 36.2 205.0 207.0 Example 6 7247 7238 24.6 28.5 294.6 254.0 Comparative Example 1 7351 7252 234.5 342.3 31.3 21.2 Comparative Example 2 7523 7234 195.2 156.2 38.5 46.3 Comparative Example 3 7240 7562 143.9 234.3 50.3 32.3 Comparative Example 4 7345 7062 95.2 98.5 77.2 71.7 Comparative Example 5 7295 7432 129.3 231.1 56.4 32.2 Comparative Example 6 7832 7231 78.2 109.2 100.2 66.2 Comparative Example 7 7452 7523 76.2 113.5 97.8 66.3

As can be seen in Table 4, the disinfectants of Examples 1 to 7 showed a large SI value, confirming that the inhibitory effect of the virus was very good compared to the disinfectants of Comparative Examples 1 to 7.

<Experimental Example 3. Sensory evaluation>

Sensory evaluation of the hand sanitizer prepared in the gel state of the disinfectants of Examples 1 and 7 was performed. The degree of preference for fragrance was rated as 5 points: the best, 4 points: good, 3 points: average, 2 points: bad, 1 point: very bad. As a result, as shown in Table 5 below, the disinfectant of Example 6 containing citron essential oil showed good preference for fragrance, and the citron essential oil had an antiviral effect while improving the preference. It was confirmed that damage and the like had no effect at all.

gel disinfectant preference for incense skin burning sensation Example 1 3.2 doesn't exist Example 6 4.4 doesn't exist

<Experimental Example 4. Confirmation of antibacterial effect>

E. coli 0-157 ( Escherichia coli 0-157), a pathogenic bacterium, was cultured in 3% (w/v) TSB (trypticase soy broth) at 37° C. and 200 rpm for 18 hours, and then again 4×10 ⁶ CFU under the same conditions. (colony forming units) / ㎖ concentration was secondary culture for 2 hours 30 minutes. Then, sterilized citrate phosphate buffer (9mM sodium phosphate, 1mM sodium citrate, pH 7.4) and 1% (w/v) type I (low electroendosmosis) agarose, 0.03% (w/v) TSB Add cultured bacteria (4×10 ⁶ colony forming units/ml) to 10mL of the gel, mix, and pour on a square plate. When the gel hardens, it is prepared with the disinfectant of Examples 1-6 and Comparative Examples 1-7 A PET film (thickness 0.1mm) specimen coated with a gel for hand disinfection was cut out to a circular diameter of 10mm and spread out. After 2 days of incubation, it was checked whether a clear zone was formed, and the size of the clear zone was measured from the circular end of the specimen in the outward direction and measured and described in Table 6 below.

Gel Formulation Disinfectant Specimens E. coli (mm) Example 1 26 Example 2 26 Example 3 25 Example 4 25 Example 5 26 Example 6 34 Comparative Example 1 15 Comparative Example 2 13 Comparative Example 3 15 Comparative Example 4 16 Comparative Example 5 17 Comparative Example 6 18 Comparative Example 7 15

As a result of the measurement, since ethanol is basically included in each specimen sample, the antibacterial effect of the specimens of Comparative Examples 1 to 7 is confirmed. Compared with Comparative Examples 1 to 7, the antibacterial effect of Examples 1 to 6 is significantly higher. appears to be better.

Claims (6)

An antiviral disinfectant comprising a plum sugar extract, a poultry berry extract, an acacia extract, a coltsfoot extract, a Chinese cabbage extract, and an asparagus extract as active ingredients. delete The method of claim 1,
Antiviral disinfectant, characterized in that the disinfectant contains yuja essential oil. The method of claim 1,
Plum sugar extract is an antiviral disinfectant, characterized in that it is a liquid obtained by osmotic extraction by leaving a mixture of sugar mixed with plum. The method of claim 1,
The disinfectant is an antiviral disinfectant, characterized in that it has an antibacterial effect. The method of claim 1,
The disinfectant is an antiviral disinfectant, characterized in that the gel-type or spray-type formulation.