KR102345209B1 - Sanitizer composition has excellent anti-viral effect, sterilization effect and skin moisturizing effect - Google Patents

Abstract

The present invention provides an excellent disinfectant composition which exhibits sufficient bactericidal and virucidal effects even if alcohol content is lowered by using one selected from a group consisting of Kayu Bajakah, Phalaenopsis spp. petals, and a mixture thereof, and in addition, heals simple wounds with the moisturizing effect of protecting the skin and the chemical properties of Kayu Bajakah. According to the present invention, the alcohol content is lowered, and it is possible to manufacture a product having a quality higher than a level required by a consumer by further comprising an antibacterial component or antiviral component, a skin moisturizing component or skin trouble prevention component, and a component for formulation stability.

Description

Antiseptic composition having excellent antiviral, bactericidal and skin moisturizing effect

The present invention relates to a disinfectant composition having excellent virucidal, bactericidal and skin moisturizing effects.

Conventionally, it is generally known that lower alcohols having 1 to 3 carbon atoms, such as ethanol and isopropyl alcohol, have a disinfecting effect on bacteria and viruses by destruction of bacterial cell membranes or virus envelopes, or coagulation of proteins such as enzymes. Disinfectants containing lower alcohols are widely used.

However, in general, disinfectants containing lower alcohols such as ethanol and isopropyl alcohol have a problem of causing roughness of the user's hand, for example, when used to disinfect hands. Roughness of the hand indicates a state in which the skin barrier function is lost due to the removal of the stratum corneum and sebum film and the loss of the skin barrier function. From this point of view, there is a demand for a disinfectant excellent in moisturizing properties that can prevent roughness of the hand, that is, a defect in the skin barrier function, even when a lower alcohol is blended.

In order to solve the above problem, according to Korean Patent Laid-Open Patent No. 10-2012-0063036, etc., a composition containing an oriental medicine ingredient and a natural ingredient having an excellent skin moisturizing effect is provided in lower alcohol, but in this case, the lower alcohol in the composition content As the content of is reduced, the sterilization or virucidal effect is reduced, resulting in a problem that cannot faithfully perform its role as a disinfectant.

1. Republic of Korea Patent Publication No. 10-2012-0063036

Therefore, the problem to be solved by the present invention is that, if it is useful for moisturizing the skin, the sterilizing and virucidal effect of the composition is sufficiently exhibited even if the content of the lower alcohol is lowered by containing a component that enhances the bactericidal and virucidal effect of lower alcohol. That is, it relates to a disinfectant composition having very excellent skin moisturizing effect, bactericidal effect and bactericidal effect.

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In order to achieve the above object, the present invention
40 to 60% by weight of absolute ethanol;
20% by weight of a paleenopsis petal extract obtained by extracting dried Palenopsis petals with purified water at 60 to 70° C. for 1 to 3 hours; and
It provides a disinfectant composition comprising the remainder by weight of purified water.

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As described above, the disinfectant composition according to the present invention may further include a basaka root extract, which is an extract undiluted solution extracted by pressing basaka roots, or a basaka wood extract, which is an extract obtained by extracting dried basaka wood with steam for 8 to 14 hours. have.

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According to the present invention, by using one selected from the group consisting of bajaca, paleenopsis petals, and mixtures thereof, sufficient sterilization and bactericidal effects are exhibited even when the alcohol content is lowered, and the moisturizing effect of protecting the skin and It is possible to provide an excellent antiseptic composition for healing simple wounds with chemical properties, and according to the present invention, which can lower the alcohol content in this way, an antibacterial or antiviral component other than alcohol, a skin moisturizing or skin trouble prevention component, a component for formulation stability It is possible to manufacture products with a quality higher than the level required by consumers by including more.

Hereinafter, specific details for the implementation of the disinfecting composition of the present invention will be described in detail.

In order to provide a disinfectant composition having very excellent skin moisturizing effect, sterilization effect and virucidal effect, the present invention is an extract undiluted solution extracted by pressing basaka roots in lower alcohol having 1 to 3 carbon atoms, and dried basaka wood with steam for 8 to It provides a disinfectant composition further comprising any one or more selected from the group consisting of an extract extracted for 14 hours, and an extract obtained by extracting Palenopsis petals with purified water at 60 to 70° C. for 1 to 3 hours.

In the present invention, as long as the disinfectant composition contains a lower alcohol having 1 to 3 carbon atoms as defined in the present invention, any formulation known in the art, such as a spray form or a gel form, is irrelevant, and additives such as excipients used to make the formulation can of course contain.

The lower alcohols having 1 to 3 carbon atoms in the present invention are alcohols having 1 to 3 carbon atoms, such as methanol, ethanol, and isopropyl alcohol. Or by killing the virus, it is possible to prevent adverse effects on the human body, such as diseases caused by the bacteria or virus.

However, such lower alcohols having 1 to 3 carbon atoms evaporate together with the moisture of the skin and cause skin damage such as rough skin and an unpleasant odor problem. have.

In the present invention, in order to solve this problem, the sterilization and virucidal effect are strengthened by using the basaka root undiluted solution, the basaka wood extract, and the palenopsis petal extract, and the unpleasant odor problem of alcohol is also prevented while maintaining skin moisture and preventing skin damage. characterized by being resolved.

There is no particular limitation on the method of using the basaka root, basaka wood, and palenopsis petals, but the present inventors' various test results,

Bajaca root is a juice obtained using a press,

Bajaca wood is an extract extracted for 8 to 14 hours with steam made using purified water after drying.

It was confirmed that it is preferable to use the paleenopsis petals as an extract extracted with purified water at 60 to 70° C. for 1 to 3 hours.

The Bajaca is a tree growing in Kalimantan and known for its anticancer effect, and the Phalaenopsis is a plant of the Orchidaceae called Phalaenopsis Orchid. Contained in the

The content of the above-mentioned bajaca extract and paleenopsis petal extract is not particularly limited, but is preferably 10 to 30% by weight based on the total composition, and when it is less than 10% by weight, the effect is insignificant, and the content exceeds 30% by weight. In this case, the content of alcohol or other excipients is relatively reduced, which may cause problems in formulation stability.

In addition, although there is no particular limitation, in the present invention, the content of the lower alcohol having 1 to 3 carbon atoms is preferably 40 to 60% by weight based on the entire composition, and the composition of the present invention is in the technical field to which the present invention belongs in addition to the above-mentioned components. A well-known moisturizer ingredient, deodorant or fragrance ingredient that can alleviate the odor of lower alcohol can be used without any particular limitation, and for this reason, the content of lower alcohol, which has a disinfecting effect on bacteria and viruses, is 40% by weight. This is because of the characteristic of the present invention that it can be lowered to .

Examples of the above-described moisturizing ingredients include extracts of gold, astragalus, solanum, green tea, gold damsel, eumnamu, and Heotgae tree, which are natural herbal ingredients in the present invention, and aloe vera gel, hyaluronic acid, essential oil, and the like can also be used.

Another example is fats and oils, which are liquid or solid fats and oils at room temperature of 20°C to 25°C. By suppressing volatilization, roughness of the hand, etc. can be prevented, and as oil and fat, for example, hydrocarbons such as liquid paraffin, light liquid paraffin, and squalane, isopropyl palmitate, octyl palmitate, iso myristic acid Ester oil such as propyl, isononyl isononanoate, medium-chain fatty acid triglyceride composed of fatty acid having 8 to 22 carbon atoms, tri(caprylic/capric acid) glyceryl, myristyl alcohol (24 carbon atoms), cetanol (carbon number) 16), stearyl alcohol (18 carbon atoms), cetostearyl alcohol (a mixture of 16 and 18 carbon atoms), behenyl alcohol (22 carbon atoms), isostearyl alcohol (18 carbon atoms), 2-hexyldecanol (16 carbon atoms) , aliphatic alcohols such as saturated monohydric alcohols such as , 2-octyldodecanol (20 carbon atoms), unsaturated monohydric alcohols such as oleyl alcohol (18 carbon atoms), soybean oil, safflower oil, grape seed oil, rosehip oil, sunflower oil Oil, evening primrose oil, cottonseed oil, sesame oil, wheat germ oil, almond oil, etc. are preferred. Non-drying oils include olive oil, sandalwood oil, camellia oil, castor oil, peanut oil, avocado oil, macadamia nut oil, hazelnut oil, meadow foam oil. , jojoba oil and the like are preferred, and the essential oil includes eucalyptus oil, peppermint oil, and Roman chamomile oil (vegetable oil).

Examples of other moisturizing agents include polyhydric alcohols such as glycerin, polyglycerin, propylene glycol, 1,3-butylene glycol, 1,2-pentanediol, 1,2-hexanediol, polyethylene glycol, maltitol, and sorbitol. , sodium hyaluronate, urea, sodium lactate, trimethylglycine, and the like.

In addition, in the present invention, a thickener may be blended as a component for increasing the viscosity of the disinfecting composition as needed. Examples of the thickener include carboxyvinyl polymer, carboxymethyl cellulose, hydroxyethyl cellulose, which is a cross-linked polyacrylic acid. , cellulose derivatives such as hydroxypropyl cellulose, xanthan gum, guar gum, gum arabic, sodium alginate, propylene glycol alginate, ethyl cellulose, sodium polyacrylate, and cyclodextrin.

In addition, in the present invention, if necessary, the disinfecting composition can be uniformly mixed with a surfactant to stabilize the creamy appearance, and it is possible to prevent the separation of lower alcohols having 1 to 3 carbon atoms, etc., which are components of the disinfecting composition. , As surfactant, cationic surfactant, anionic surfactant, amphoteric surfactant, and nonionic surfactant are mentioned, for example.

In addition, in the present invention, a disinfecting component may be further formulated to disinfect bacteria or viruses that cannot be sufficiently disinfected with alcohol, for example, benzethonium chloride, benzalkonium chloride, chlorhexidine gluconate, povidone-iodine, iso propylmethylphenol, chloroxylenol, and the like.

Next, the present invention will be described in detail through Examples and Test Examples.

[ Example 1: Preparation of disinfectant composition]

A disinfectant composition according to the present invention was prepared by adding 20% by weight of an extract stock solution obtained by pressing basaka root, 40% by weight of absolute ethanol, and purified water of the remainder by weight.

[ Example 2: Preparation of composition for disinfection]

A disinfectant composition according to the present invention was prepared in the same manner as in Example 1, except that an extract extracted by boiling for 10 hours with steam made by heating purified water on dried basaka wood instead of basaka root was used, at this time The steam was equal in weight to the dry bazaka wood.

[ Example 3: Preparation of disinfectant composition]

A disinfectant composition according to the present invention was prepared in the same manner as in Example 1, except that the dried Palenopsis petals were placed in purified water heated to 70° C. instead of the bajaca root and the extract extracted for 1.5 hours was used, in which case the purified water used was twice the weight of the paleenopsis petals.

[ Example 4: Preparation of disinfectant composition]

A disinfectant composition according to the present invention was prepared in the same manner as in Example 1, except that 60% by weight of absolute ethanol was used.

[ Example 5: Preparation of composition for disinfection]

A disinfectant composition according to the present invention was prepared in the same manner as in Example 2, except that 60% by weight of absolute ethanol was used.

[ Example 6: Preparation of disinfectant composition]

A disinfectant composition according to the present invention was prepared in the same manner as in Example 3, except that 60% by weight of absolute ethanol was used.

[ test example 1: Moisture]

Moisture retention evaluation of the prepared disinfecting composition was measured using a MY-808S Moisture Checker (MY-808S Moisture Checker) before and after applying the Example (30 minutes elapsed), and the results are shown in Table 1, The results in Table 1 are the results of measuring and average of 5 female participants in their 20s per one test, and the control group used a disinfectant composition containing 10 parts by weight of aloe vera with respect to 100 parts by weight of an 80% by weight aqueous solution of ethanol.

Moisture content (%) Jeon after Example 1 30.87 38.81 Example 2 30.88 39.83 Example 3 30.87 40.11 Example 4 30.89 34.68 Example 5 30.88 35.14 Example 6 30.87 37.46 control 30.87 25.19

Referring to Table 1, all of the Examples according to the present invention showed a higher skin moisture content after application than before application, and in the control group, the skin moisture content was decreased due to the use of Aloe vera, indicating that the effect of the present invention is remarkable was able to confirm

[ test example 2: Sterilization effect]

In evaluating the sterilization effect of the prepared disinfecting composition, the sample and the bacterial solution (Salmonella typhimurium) were directly contacted and spread on a plate medium, and the number of bacteria was evaluated. As an evaluation criterion, the ratio of the number of bacteria killed by contact with the disinfecting composition to the number of controlled bacteria is an effect of 99.99% or more, an “A” evaluation, an effect of less than 99.99% is an “C” evaluation, and “ A” evaluation was preferred and “C” evaluation was evaluated as undesirable, and the results are shown in Table 2, and the control group is the same as the moisture retention test.

Sterilization Example 1 A Example 2 A Example 3 A Example 4 A Example 5 A Example 6 A control A

As shown in Table 2, in the case of Examples according to the present invention, the same as the control using an aqueous solution having an alcohol content of 80% by weight was evaluated excellently in sterilization, and in particular, the composition of the present invention lowered the alcohol content to 40% by weight. It was possible to confirm the inventive step of the present invention by showing an excellent effect even in the state.

[ test example 3: virucidal virus validity]

Influenza virus type A (H1N1) and seasonal influenza virus type A (H3N2) were infected with MDCK (Mardin Darby Canine Kidney) cell lines, and then treated with the compositions of Examples and Comparative Examples to analyze antiviral activity.

The MDCK cell line used in the experiment was routinely cultured in Eagle's minimum essential medium (MEM) containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin solution (Gibco). prepared

1 x 10 ⁴ MDCK cells per 1 ml were dispensed in a 96-well plate and cultured at _{37° C. for 16 hours using a 5% CO 2 incubator.}

After washing the monolayer MDCK cells twice with PBS, influenza virus type A (H1N1) and seasonal influenza virus type A (H3N2) were infected with 100 TCID50 at 37°C for 2 hours, and then uninfected viruses were washed away. .

Infected cells were sequentially diluted with a virus culture solution (0.3% Bovine serum albumin, 1% Penicillin-Streptomycin solution) containing the composition of Examples and Control (same composition as in the moisture test) and L-1-tosyl at 1 μg/ml. In MEM culture medium containing trypsin-TPCK treated with amido-2-phenylethyl chloromethyl ketone, it was incubated at 37° C. for 48 hours until viral CPE (cytophatic effect) appeared. The antiviral CPE lowering ability of the compositions was expressed as a value of 50% of the effective concentration of viral inhibition (EC50), and the value of 50% of the concentration of cytotoxicity (CC50) was determined based on the morphological modification of cells.

The anti-influenza virus ability of the compositions was shown in Table 3 as the selectivity index (SI) obtained by dividing the CC50 value by the EC50 value.

SI H1N1 H3N2 Example 1 92.3 90.4 Example 2 94.7 93.5 Example 3 95.7 95.8 Example 4 96.9 96.3 Example 5 97.5 96.8 Example 6 98.7 98.5 control 89.7 89.4

According to Table 3, all of the compositions according to the present invention showed an excellent virucidal effect, and in particular, more excellent effects were shown in the present invention using 40% by weight aqueous alcohol solution than in the control using 80% by weight aqueous alcohol solution, so the inventive step of the present invention was able to confirm

According to the present invention as described above, by using one selected from the group consisting of bajaca, paleenopsis petals, and mixtures thereof, sufficient sterilization and bactericidal effects are exhibited even when the alcohol content is lowered, and a moisturizing effect that protects the skin It is possible to provide an excellent antiseptic composition for healing simple wounds with the chemical properties of Waxaka, and according to the present invention, which can lower the alcohol content in this way, antibacterial or antiviral components other than alcohol, skin moisturizing or skin trouble prevention components, formulations It is possible to manufacture a product with a quality higher than the level required by consumers by further including ingredients for stability.

Claims (6)

40 to 60% by weight of absolute ethanol;
20% by weight of a paleenopsis petal extract obtained by extracting dried Palenopsis petals with purified water at 60 to 70° C. for 1 to 3 hours; and
Disinfectant composition comprising the remainder by weight of purified water.
[Claim 2] The method of claim 1, further comprising a basaka root extract, which is an extract undiluted solution extracted by pressing basaka roots, or a bazaka wood extract, which is an extract obtained by extracting dried basaka wood with steam for 8 to 14 hours. A disinfectant composition comprising
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