KR102343944B1 - Cosmetic component using extract Osmanthus insularis - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising an extract of Rubus hongnoensis Nakai or a fraction thereof as an active ingredient.

Description

Cosmetic composition using extract Osmanthus insularis {Cosmetic component using extract Osmanthus insularis}

The present invention relates to a cosmetic composition using Bakdalmokseo extract or a fraction thereof.

Superoxide radical (O2·), hydroxyl radical (OH·), hydrogen peroxide (H2O2), monoliths generated during normal cellular metabolism in cells or by exposure to various environmental pollution and chemicals Oxygen compounds such as oxygen (singlet oxygen, 1O2) are collectively referred to as reactive oxygen species (ROS) (Free Radic Biol Med 26: 202-226, 1999).

These reactive oxygen species are very reactive and react quickly with biomolecules, resulting in cell membrane damage, DNA denaturation, lipid oxidation, protein degradation, etc. It is known to increase the incidence of chronic diseases such as sclerosis (Chem. Res. 14: 393 (1981); Arch. Biophys. 247: 1-11 (1986); Science 221: 1256-1264 (1983)).

One of the ways to effectively remove free radicals in the body, a risk factor that causes these diseases, is the intake of antioxidants (Food Chem Toxicol 39: 1205- 1210, 2001). Antioxidants inhibit or minimize damage to the living body by reactive oxygen species by reacting with active oxygen. Butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT, Butylated hydroxytoluene), Synthetic antioxidants such as PG, Propyl galate, tertiarybutyl hydroquinone (TBHQ) and chlorophyll, green tea extract, prorolys, tocopherol, ascorbate, carotenoid, glutathione ) can be classified as antioxidants derived from natural products, such as synthetic antioxidants, which are known to cause side effects on carcinogenesis and cardiovascular systems. Yes (Korean J. Food Sci. Technol. 34:889-892 (2002)).

On the other hand, as the human skin is an organ in direct contact with the surrounding environment, infections such as bacteria and fungi involved in various skin diseases easily occur. For example, Staphylococcus aureus, a Staphylococcus aureus that causes skin suppuration, Staphylococcus epidermidis, a skin flora, and Propionibacterium acnes, an acne bacterium acnes) exist on the skin, and these bacteria decompose the sebum or sweat secreted on the skin to generate odor, and the decomposition products also irritate the skin and cause inflammation.

As an example of such inflammation, skin acne is a disease that affects the oil glands of the skin, and the pores are connected to the oil glands under the skin, which produce an oily substance called sebum. When the sacs of the skin glands within the pores become clogged, acne occurs. It is known that when the sacs of the skin glands in the pores become clogged, inflammation occurs, which promotes the development and colonization of the acne-promoting P. acnes bacteria.

Currently, acne treatment in dermatology is performed through vitamin A retinoid, benzoyl peroxide, and various antibiotics. In addition, salicylic acid and triclosan are used as cosmetic materials for people with oily skin, which are used based on the effect of suppressing inflammation accompanying atopy.

However, salicylic acid is a component that causes skin irritation such as itching and burning, and can cause skin dryness and peeling. In addition, triclosan also stimulates when it comes into contact with the skin and kills acne bacteria in a similar way to antibiotics, so bacteria that have developed resistance to it become resistant to other antibiotics.

Therefore, it is necessary to discover new natural functional materials applicable to people with oily skin, discover new cosmetic materials, and verify their usefulness.

Republic of Korea Patent No. 10-0574233

It is an object of the present invention to provide a cosmetic composition having antioxidant activity and antibacterial activity using an extract of Bakdalmokseo or a fraction thereof.

Other objects or specific objects of the present invention will be set forth below.

As confirmed in the Examples and Experimental Examples below, the present invention has been completed by confirming that the Bakdalmokseo extract or a fraction thereof has antioxidant activity and antibacterial activity.

The present invention is provided based on these experimental results, and the cosmetic composition of the present invention is characterized in that it contains an extract of Bakdalmokseo or a fraction thereof as an active ingredient.

Osmanthus insularis is an evergreen tree belonging to the genus Osmanthus in the family Oleaceae. It is an endangered species and rare plant designated by the Ministry of Environment. It is distributed in Korea, Japan, and Taiwan. (Korea Forest Service Forestry Research Institute, 1996, Rare and Endangered Plants: Conservation Guidelines and Target Plants).

It is an evergreen tree with a height of 15m, the bark is gray, and the branches are rather flat. The leaves are opposite, long oval or ovate long oval, the tip of the leaf is acute or pointed, the base is acute, and the length is 7 - 12 cm. The edges are not serrated, but in young trees there are sometimes saw teeth. The surface is free from veins and the petiole is 1.5 - 2.5 cm long. Flowers bloom in October - December in white, grow in the leaf axil, and the pedicel is 7 - 10 mm long, and the calyx lobe is triangular and has serrations. The fruit is oval, 1.5 - 2cm long, and ripens to black around May of the next year.

In the present specification, "Bakdalmokseo extract" refers to an extraction target (Bakdalmokseo), water, methanol, ethanol, lower alcohols having 1 to 4 carbon atoms such as butanol, methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, Extracts obtained by leaching using butyl acetate, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol or a mixed solvent thereof, carbon dioxide, pentane, etc. supercritical It refers to an extract obtained by using an extraction solvent or a fraction obtained by fractionating the extract, and the extraction method is arbitrary, such as chilling, reflux, heating, ultrasonic radiation, supercritical extraction, etc. method can be applied. In the case of a fractionated extract, a fraction obtained by suspending the extract in a specific solvent and mixing and standing still with a solvent having a different polarity, and adsorbing the extract to a column filled with silica gel, etc. It is meant to include the fraction obtained as a mobile phase. In addition, the meaning of the extract includes a concentrated liquid extract or solid extract from which the extraction solvent is removed by methods such as freeze drying, vacuum drying, hot air drying, spray drying, and the like. Preferably, it refers to an extract obtained by using water, ethanol, or a mixed solvent thereof as an extraction solvent, and more preferably an extract obtained by using a mixed solvent of water and ethanol as an extraction solvent.

In the present specification, "Bakdalmokseo fraction" refers to extracting Bakdalmokseo with a solvent as mentioned above, filtering the extracted extract and concentrating under reduced pressure to obtain a Bakdalmokseo extract, which is then used in hexane, dichloromethane, ethyl acetate, butanol. It can be obtained by sequential fractionation using Preferably, it may be an ethyl acetate fraction.

Also, in the present specification, the term "active ingredient" means a component that can exhibit the desired activity alone or can exhibit activity together with a carrier having no activity by itself.

The cosmetic composition of the present invention may be prepared including, in addition to the active ingredient, a skin whitening component, a skin wrinkle improving component, a UV protection component, a skin moisturizing component, etc. known in the art in order to reinforce/add skin-related activity. Specifically, as a skin lightening component, arbutin, niacinamide, ascorbyl glucoside, alpha-bisabolol, oil-soluble licorice extract, etc. may be mentioned, and as a skin wrinkle improving component, retinol, retinyl palmi tate, adenosine, polyethoxylated amide and the like. and complex extracts such as dotriazone and pine bark extract, phosphatidylserine, finger root extract powder, and complex extracts such as red ginseng and cornus oil. In addition, as moisturizing ingredients, AP collagen enzyme decomposing peptide, Collactive collagen peptide, N-acetylglucosamine, konjac potato extract, dandelion complex extract, rice bran extract, corn germ extract, low molecular weight collagen peptide, ground grass extract powder, phosphatidylserine, hyaluronic acid, etc. can be heard For other skin whitening ingredients, wrinkle improvement ingredients, UV protection ingredients, and skin moisturizing ingredients, the Functional Cosmetics Ordinance in accordance with the Cosmetics Act (Ministry of Food and Drug Safety Notification “Functional Cosmetics Standards and Test Methods”), health functional food in the Health Functional Food Act Reference may be made to the Korean Code (Ministry of Food and Drug Safety Notification “Health Functional Food Standards and Specifications). One or more of these ingredients may be included in the composition for skin whitening of the present invention together with the active ingredient.

In the cosmetic composition of the present invention, the active ingredient may be included in any amount within the range that does not have toxicity to the human body depending on the use, formulation, etc., specifically, 0.001 wt % to 99.99 wt % relative to the total weight of the composition can be included as

The cosmetic composition of the present invention may be classified into any product in terms of its use and by law, and specifically may be functional cosmetics, non-functional general cosmetics, etc. having a use such as skin whitening. In terms of product form, it may take any product form, and specifically, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax It may be formulated as a foundation, a spray, or the like. In a specific product form, it may be in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-Butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan, etc. may be used.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, polyoxyethylene sorbitan ester, microcrystals Adult cellulose, aluminum metahydroxide, bentonite, agar, etc. can be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester and the like may be used.

As described above, according to the present invention, it is possible to provide a cosmetic composition having antioxidant activity and antibacterial activity by using the extract and fractions thereof.

1 is a graph showing the results of measuring the electron donating ability of the ethanol extract and fractions of Bakdalmokseo.
Figure 2 is a graph showing the results of measuring the ABTS radical donating ability of the ethanol extract and fractions of Bakdalmokseo.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these Examples and Experimental Examples.

<Example> Preparation of extracts and fractions thereof

Bakdalmokseo planted at the Nanah Tropical Forest Science Research Center in Seogwipo, Jeju Special Self-Governing Province was collected, dried in the shade, and crushed with a grinder. The fine powder sample was leached in 70% ethanol at room temperature for 48 hours and extracted three times, after which the supernatant was recovered and concentrated under reduced pressure to obtain an ethanol extract. And after suspending 10 g of the ethanol extract in 1 liter of distilled water, each fraction was extracted with hexane (1 ℓ × 3), dichloromethane (1 ℓ × 3), ethyl acetate (1 ℓ × 3), butanol (1 ℓ × 3) It was vacuum dried to obtain hexane (Hexane), dichloromethane (CH ₂ Cl ₂ ), ethyl acetate (EtOAc) and butanol (BuOH), and a residue (Water). Each fraction was dissolved in the corresponding solvent, sterilized with a 0.2 μm membrane filter (Advantec MFS Inc. USA), and stored at 4°C, and used in this study.

<Strains and reagents used>

As for the strains used in this experiment, 3 strains commonly detected in the skin and antibiotic-resistant bacteria of Staphylococcus epidermidis and Propionibacterium acnes were selected and used (Table 1). Propionibacterium acnes was anaerobic and cultured in anaerobic Jar (Oxoid, USA). The medium of these strains was purchased from Difco (USA) for Tryptic soy agar (TSA) and GAM Broth from Nissui (Japan) for use.

<Experimental Example 1> Measurement of antioxidant activity

<1> Experimental method

<1-1> Electron donating ability

Electron donating abilities (EDA) of Bakdalmokseo extract and fractions were measured by the electron donating effect of α,α-diphenyl-β-picrylhydrazyl (DPPH) on the extract, and the reducing power of each extract was measured. 100 μl of DPPH solution and 100 μl of extracts and fractions for each concentration were mixed, and the reaction was induced in a cool and dark room for 30 minutes. Then, the concentration of the remaining radicals was measured at 520 nm using a UV spectrophotometer. Electron donating ability was shown by the decrease in absorbance between the addition and non-addition samples of the sample solution.

<1-2> ABST radical donating ability

2,2'-Azion-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activity was measured by preparing 7.4 mM ABTS and 2.6 mM potassium persulfate, and then prepared by concentration in 100 μl of diluted ABTS·+ solution. After adding 100 μl of the extract, the change in absorbance at 700 nm was measured after 30 minutes. The antioxidant activity was expressed as a percentage of the radical scavenging ability of the control group using ethanol, a solvent in which the sample was dissolved.

<2> Experimental results

<2-1> Electron donating ability measurement result

Reduction of DPPH can predict the degree of inhibition of the initial reaction of lipid peroxidation as the scavenging reaction of free radicals proceeds. It is known that active oxygen, a harmful oxygen, attacks unsaturated fatty acids, a component of the cell membrane, and causes a lipid peroxidation reaction to accumulate lipid peroxide in the body. T. 1995. Constituents of natural medicines with scavenging effects on active oxygen species tannins and related polyphenols. Nat. Med. 49: 357-363.).

The DPPH assay used in the electron donating ability measurement experiment can be said to be a method using the principle that purple is discolored by receiving hydrogen or electrons and reducing it by phenolic compounds, aromatic amines, and ascorbic acid. It is mainly used to identify antioxidants and has been widely used because it is relatively simple and can be measured within a short time (Riley PA. 1997. Molecules in focus melanin. Int. J. Biochem. Cell Biol. 29: 1235-1239.) .

As a result of measuring the electron donating ability of the ethanol extract and fractions of Bakdalmokseo, the electron donating ability increased as the concentration increased, as shown in FIG. 1, and showed an effect of 80% or more at 50 μg/ml, which was excellent when compared with ascorbic acid, a control group. It was confirmed that the electron donating activity was exhibited. In particular, the dichloromethane fraction exhibited an effect of 80% or more at 12.5 μg/ml, and showed excellent electron donating activity at a lower concentration than the ethanol extract and other fractions.

<2-1> ABST radical donating ability measurement result

In the same principle as the DPPH radical scavenging activity experiment, the ABTS assay is a method to measure antioxidant activity by using the discoloration of blue-green color while ABTS·+ in the nature of peroxide radical generated by the reaction with potassium persulfate is removed by antioxidant substances. This is a method using radical scavenging (Li H, Choi YM, Lee JS, Park JS, Yeon KS, Han CD. 2007. Drying and antioxidant characteristics of the Shiitake (Lentinus edodes) mushroom in a conveyer-type far-infrared dryer J Korean Soc Food Sci Nutr 36: 250-254).

Since ABTS reagent is soluble in both synthesized radicals, organic solvents and water, it can be used to measure the antioxidant activity of non-polar samples as well as polar samples, and has been widely used in the study of antioxidant activity (Awika JM, Rooney LW, Wu X, Prior RL, Cisneros). -Zevallos L. 2003. Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products. Agric. Food Chem. 51:6657-6719.). The results of measuring the ABTS electron donating ability of the ethanol extract and fractions from Dalmokseo Park are shown in FIG. 2 . As the concentration increased, the electron donating ability increased, and 80% at 12.5 μg/ml and over 90% scavenging at 100 μg/ml were exhibited. It was confirmed that it was significant with the result of ascorbic acid, a control material.

<Experimental Example 2> Measurement of antibacterial activity

<1> Experimental method

Antimicrobial activity was measured using the disc diffusion assay method (Hayes, AJ, Markovic, B., 2002. Toxicity of Australian essential oil Backhousia citriodora (Lemon myrtle). Part 11. Antimicrobial activity and in vitro cytotoxicity. Food. Chem. Toxicol. 40(4): 535-543). Each strain was inoculated in 10 mL of liquid medium and passaged 3 times at 37°C for 18 hours to be used as a test strain for antibacterial activity. Plate method was used to make and use a plate medium for inoculation. Each sample is prepared at a concentration of 50 mg/ml, slowly absorbed on a paper disc (Whatman No. 5, 8 mm) by concentration, and then the solvent is completely evaporated. After adhering the disc to the surface of the test plate medium and culturing at the optimum temperature of 37°C, the size of the growth inhibitory ring (clear zone, mm) generated around the disc was measured to compare the antibacterial activity. Table 1 below summarizes the strains and media used.

<2> Experimental results

Antibacterial activity according to treatment concentration was measured by disc diffusion assay method for ethanol extracts and sequential fractions of Bakdalmokseo. As shown in Table 2 below, among the ethanol extract and sequential fractions, S. aureus was active in hexane, dichloromethane, and ethyl acetate fractions, and the dichloromethane fraction showed higher activity than other fractions. S. epidermidis SK4 and antibiotic-resistant bacteria S. epidermidis SK9 and S. epidermidis SK19 were active in ethanol extracts and dichloromethane fractions, and P. acnes and P. acnes SK7 were active in hexane, dichloromethane, and ethyl acetate fractions. took on

1) -, No inhibition

<Experimental Example 3> Minimum inhibitory concentration (MIC) measurement of extracts and fractions

<1> Experimental method

The minimum inhibitory concentration (MIC) was determined according to the micro-well dilution assay method (Amster D. 1996. Susceptibility testing of antimicrobial in liquid media, antibiotics in laboratory medicine. 4th ed. Williams and Wikins, MD, USA. p 52-111). measured. After making the concentration of each bacterial cell to 106-108 CFU/mL with 0.5 McFarland, it was aliquoted into a 96-well plate, and each sample was treated with 10 μL of each concentration and cultured. The degree of proliferation of the bacterial culture was measured at 650 nm with a Microplate reader (Powerwave XS, BioTek). The minimum inhibitory concentration was determined by comparing the absorbance values in the wells inoculated only with bacteria.

<2> Experimental results

Minimum inhibitory concentration (MIC) was measured for the ethanol extract and sequential fractions of Bakdalmokseo. As a result, as shown in Table 3 below, S. aureus was inhibited at a low concentration of 0.25 mg/ml in hexane and dichloromethane fractions. S. epidermidis SK4, S. epidermidis SK9, and S. epidermidis SK19 were inhibited at a low concentration of 0.312 mg/ml in the dichloromethane fraction, and 0.125 - 0.250 mg/ml in the ethanol extract, hexane and ethyl acetate fractions. became As with the results of the disc diffusion assay, P. acnes had insignificant antibacterial activity in the ethanol extract, but showed inhibitory concentrations in sequential fractions of hexane, dichloromethane, and ethyl acetate fractions. In particular, P. acnes was inhibited at a low concentration of 0.312 mg/ml in the ethyl acetate fraction.

1) -, No growth

<Experimental Example 4> Minimum bactericidal concentration (MBC) measurement

<1> Experimental method

100 ul each of the culture solution in the well corresponding to a concentration higher than the concentration selected as MIC was directly smeared on the solid medium. After culturing each bacteria, the number of colonies visually observed on the plate was directly counted. If the number of colonies identified at this time was confirmed to have the effect of killing 99.9% of the initial number of inoculated bacteria inoculated from the liquid culture medium, the treatment concentration at this time was set as the minimum bacterial lethal concentration (MBC) (Cohen MA, Huband MD, Yoder) SL, Gage JW, Roland GE., 1998, Bacterial eradication by clinafloxacin, CI-990, and ciprofloxacin employing MBC test, in-vitro time-kill and in-vivo time-kill studies.J Antimicrob Chemother.41(6): 605-14).

<2> Experimental results

The minimum bacterial lethal concentration (MBC) was measured for the ethanol extract and sequential fractions of Bakdalmokseo. As a result, as shown in Table 4 below, S. aureus showed a concentration of 0.125 mg/ml in the dichloromethane fraction, and S. epidermidis SK4, S. epidermidis SK9, S. epidermidis SK19 was 0.612 mg/ml in the dichloromethane fraction. concentration of . P. acnes and P. acnes SK7 showed a concentration of 0.25 mg/ml in the dichloromethane fraction, and P. acnes SK7 showed a concentration of 0.125 mg/ml in the ethyl acetate fraction.

1)-, No growth

Claims (3)

A cosmetic composition comprising a dichloromethane fraction of an extract of Bakdalmokseo (Osmanthus insularis) as an active ingredient.
According to claim 1,
The extract is a cosmetic composition using an extract of Bakdalmokseo, characterized in that it is obtained by using water, ethanol, or a mixed solvent thereof.
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