KR102332522B1 - 삼차원 dna 구조에서 뉴클레오티드 서열의 상호작용을 분석하기 위한 방법 - Google Patents
삼차원 dna 구조에서 뉴클레오티드 서열의 상호작용을 분석하기 위한 방법 Download PDFInfo
- Publication number
- KR102332522B1 KR102332522B1 KR1020167015969A KR20167015969A KR102332522B1 KR 102332522 B1 KR102332522 B1 KR 102332522B1 KR 1020167015969 A KR1020167015969 A KR 1020167015969A KR 20167015969 A KR20167015969 A KR 20167015969A KR 102332522 B1 KR102332522 B1 KR 102332522B1
- Authority
- KR
- South Korea
- Prior art keywords
- dna
- interaction
- interactions
- restriction enzyme
- nucleotide sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000003993 interaction Effects 0.000 title claims abstract description 214
- 108020004414 DNA Proteins 0.000 title claims abstract description 162
- 238000000034 method Methods 0.000 title claims abstract description 128
- 108091028043 Nucleic acid sequence Proteins 0.000 title claims abstract description 36
- 239000012634 fragment Substances 0.000 claims abstract description 122
- 108091008146 restriction endonucleases Proteins 0.000 claims abstract description 108
- 239000002773 nucleotide Substances 0.000 claims abstract description 56
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 56
- 108091034117 Oligonucleotide Proteins 0.000 claims abstract description 33
- 238000004132 cross linking Methods 0.000 claims abstract description 29
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000003776 cleavage reaction Methods 0.000 claims abstract description 12
- 230000007017 scission Effects 0.000 claims abstract description 12
- 210000004027 cell Anatomy 0.000 claims description 95
- 108010077544 Chromatin Proteins 0.000 claims description 68
- 210000003483 chromatin Anatomy 0.000 claims description 68
- 238000012163 sequencing technique Methods 0.000 claims description 68
- 108090000623 proteins and genes Proteins 0.000 claims description 51
- 239000000523 sample Substances 0.000 claims description 47
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims description 44
- 239000002751 oligonucleotide probe Substances 0.000 claims description 44
- 239000011324 bead Substances 0.000 claims description 36
- 201000010099 disease Diseases 0.000 claims description 31
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 31
- 210000000349 chromosome Anatomy 0.000 claims description 30
- 230000029087 digestion Effects 0.000 claims description 26
- 238000002493 microarray Methods 0.000 claims description 22
- 239000000835 fiber Substances 0.000 claims description 21
- 102000004190 Enzymes Human genes 0.000 claims description 20
- 108090000790 Enzymes Proteins 0.000 claims description 20
- 230000002068 genetic effect Effects 0.000 claims description 20
- 239000003795 chemical substances by application Substances 0.000 claims description 15
- 230000000694 effects Effects 0.000 claims description 14
- 230000014509 gene expression Effects 0.000 claims description 13
- 238000011179 visual inspection Methods 0.000 claims description 10
- 101710163270 Nuclease Proteins 0.000 claims description 9
- 210000001519 tissue Anatomy 0.000 claims description 8
- 102000040945 Transcription factor Human genes 0.000 claims description 7
- 108091023040 Transcription factor Proteins 0.000 claims description 7
- 208000011580 syndromic disease Diseases 0.000 claims description 7
- 238000010008 shearing Methods 0.000 claims description 6
- 238000012800 visualization Methods 0.000 claims description 6
- 238000012165 high-throughput sequencing Methods 0.000 claims description 5
- 238000003556 assay Methods 0.000 claims description 4
- 230000008859 change Effects 0.000 claims description 4
- 238000003745 diagnosis Methods 0.000 claims description 4
- 238000005094 computer simulation Methods 0.000 claims description 3
- 150000002500 ions Chemical class 0.000 claims description 3
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 claims description 2
- 208000026350 Inborn Genetic disease Diseases 0.000 claims description 2
- 208000024556 Mendelian disease Diseases 0.000 claims description 2
- 206010028980 Neoplasm Diseases 0.000 claims description 2
- 230000004888 barrier function Effects 0.000 claims description 2
- 238000007622 bioinformatic analysis Methods 0.000 claims description 2
- 201000011510 cancer Diseases 0.000 claims description 2
- 239000012212 insulator Substances 0.000 claims description 2
- 238000004393 prognosis Methods 0.000 claims description 2
- 230000005855 radiation Effects 0.000 claims description 2
- 208000028782 Hereditary disease Diseases 0.000 claims 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 45
- 238000009396 hybridization Methods 0.000 description 41
- 102100021519 Hemoglobin subunit beta Human genes 0.000 description 33
- 108091005904 Hemoglobin subunit beta Proteins 0.000 description 33
- 230000001605 fetal effect Effects 0.000 description 28
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 27
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 24
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 22
- 238000002474 experimental method Methods 0.000 description 20
- 239000000463 material Substances 0.000 description 20
- 239000000872 buffer Substances 0.000 description 19
- 238000004458 analytical method Methods 0.000 description 18
- 229940088598 enzyme Drugs 0.000 description 18
- 239000006228 supernatant Substances 0.000 description 18
- 108010047956 Nucleosomes Proteins 0.000 description 17
- 210000001623 nucleosome Anatomy 0.000 description 17
- 210000004940 nucleus Anatomy 0.000 description 17
- 108010014064 CCCTC-Binding Factor Proteins 0.000 description 15
- 102000016897 CCCTC-Binding Factor Human genes 0.000 description 15
- 210000003743 erythrocyte Anatomy 0.000 description 15
- 235000018102 proteins Nutrition 0.000 description 15
- 102000004169 proteins and genes Human genes 0.000 description 15
- 101001022957 Homo sapiens LIM domain-binding protein 1 Proteins 0.000 description 14
- 101001022948 Homo sapiens LIM domain-binding protein 2 Proteins 0.000 description 14
- 102100035113 LIM domain-binding protein 2 Human genes 0.000 description 14
- 210000004958 brain cell Anatomy 0.000 description 14
- 230000006870 function Effects 0.000 description 14
- 239000000203 mixture Substances 0.000 description 14
- 241000723792 Tobacco etch virus Species 0.000 description 12
- 230000008569 process Effects 0.000 description 12
- 230000001105 regulatory effect Effects 0.000 description 12
- 241000894007 species Species 0.000 description 11
- 210000004185 liver Anatomy 0.000 description 10
- 238000000746 purification Methods 0.000 description 10
- 238000004513 sizing Methods 0.000 description 10
- 241000699670 Mus sp. Species 0.000 description 9
- 210000004556 brain Anatomy 0.000 description 9
- 230000015556 catabolic process Effects 0.000 description 9
- 238000006731 degradation reaction Methods 0.000 description 9
- 238000013461 design Methods 0.000 description 9
- 238000011534 incubation Methods 0.000 description 9
- 238000005259 measurement Methods 0.000 description 9
- 239000008188 pellet Substances 0.000 description 9
- 238000005204 segregation Methods 0.000 description 9
- 230000015572 biosynthetic process Effects 0.000 description 8
- 230000002759 chromosomal effect Effects 0.000 description 8
- 238000005295 random walk Methods 0.000 description 8
- 239000007787 solid Substances 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 230000016507 interphase Effects 0.000 description 7
- 230000008520 organization Effects 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- 239000011534 wash buffer Substances 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 238000000386 microscopy Methods 0.000 description 6
- 230000004048 modification Effects 0.000 description 6
- 238000012986 modification Methods 0.000 description 6
- 102000039446 nucleic acids Human genes 0.000 description 6
- 108020004707 nucleic acids Proteins 0.000 description 6
- 150000007523 nucleic acids Chemical class 0.000 description 6
- 238000004088 simulation Methods 0.000 description 6
- 238000012421 spiking Methods 0.000 description 6
- 102000053602 DNA Human genes 0.000 description 5
- 230000033228 biological regulation Effects 0.000 description 5
- 210000003855 cell nucleus Anatomy 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000009826 distribution Methods 0.000 description 5
- 210000002257 embryonic structure Anatomy 0.000 description 5
- 238000013467 fragmentation Methods 0.000 description 5
- 238000006062 fragmentation reaction Methods 0.000 description 5
- 238000013507 mapping Methods 0.000 description 5
- 229920000642 polymer Polymers 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 238000000527 sonication Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 102100029952 Double-strand-break repair protein rad21 homolog Human genes 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 4
- 241000282412 Homo Species 0.000 description 4
- 101000584942 Homo sapiens Double-strand-break repair protein rad21 homolog Proteins 0.000 description 4
- 108020004459 Small interfering RNA Proteins 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000007983 Tris buffer Substances 0.000 description 4
- 239000012620 biological material Substances 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- DQLATGHUWYMOKM-UHFFFAOYSA-L cisplatin Chemical compound N[Pt](N)(Cl)Cl DQLATGHUWYMOKM-UHFFFAOYSA-L 0.000 description 4
- 239000003431 cross linking reagent Substances 0.000 description 4
- 238000012217 deletion Methods 0.000 description 4
- 230000037430 deletion Effects 0.000 description 4
- 230000001419 dependent effect Effects 0.000 description 4
- 238000010828 elution Methods 0.000 description 4
- 238000001976 enzyme digestion Methods 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 238000001727 in vivo Methods 0.000 description 4
- 210000005229 liver cell Anatomy 0.000 description 4
- 230000031864 metaphase Effects 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 4
- 238000002604 ultrasonography Methods 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- -1 DNA Chemical class 0.000 description 3
- 238000001712 DNA sequencing Methods 0.000 description 3
- 108010067770 Endopeptidase K Proteins 0.000 description 3
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 3
- 229930182816 L-glutamine Natural products 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 3
- 229920004890 Triton X-100 Polymers 0.000 description 3
- 239000013504 Triton X-100 Substances 0.000 description 3
- 230000006154 adenylylation Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 238000005056 compaction Methods 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 229960003722 doxycycline Drugs 0.000 description 3
- XQTWDDCIUJNLTR-CVHRZJFOSA-N doxycycline monohydrate Chemical compound O.O=C1C2=C(O)C=CC=C2[C@H](C)[C@@H]2C1=C(O)[C@]1(O)C(=O)C(C(N)=O)=C(O)[C@@H](N(C)C)[C@@H]1[C@H]2O XQTWDDCIUJNLTR-CVHRZJFOSA-N 0.000 description 3
- 238000001493 electron microscopy Methods 0.000 description 3
- 238000003197 gene knockdown Methods 0.000 description 3
- 238000007901 in situ hybridization Methods 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 238000002844 melting Methods 0.000 description 3
- 230000008018 melting Effects 0.000 description 3
- 230000011278 mitosis Effects 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 235000019419 proteases Nutrition 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 238000001890 transfection Methods 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 239000013598 vector Substances 0.000 description 3
- 230000000007 visual effect Effects 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 102000029816 Collagenase Human genes 0.000 description 2
- 108060005980 Collagenase Proteins 0.000 description 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 229920002527 Glycogen Polymers 0.000 description 2
- 108010034791 Heterochromatin Proteins 0.000 description 2
- 101001076292 Homo sapiens Insulin-like growth factor II Proteins 0.000 description 2
- 101150002416 Igf2 gene Proteins 0.000 description 2
- 102100025947 Insulin-like growth factor II Human genes 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 239000012097 Lipofectamine 2000 Substances 0.000 description 2
- NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 2
- 102000008297 Nuclear Matrix-Associated Proteins Human genes 0.000 description 2
- 108010035916 Nuclear Matrix-Associated Proteins Proteins 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 238000011529 RT qPCR Methods 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- 108010090804 Streptavidin Proteins 0.000 description 2
- 101710172711 Structural protein Proteins 0.000 description 2
- 108010076818 TEV protease Proteins 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 238000003766 bioinformatics method Methods 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 230000022131 cell cycle Effects 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 238000007385 chemical modification Methods 0.000 description 2
- YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical compound ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 2
- 108010045512 cohesins Proteins 0.000 description 2
- 229960002424 collagenase Drugs 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 238000010219 correlation analysis Methods 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000001973 epigenetic effect Effects 0.000 description 2
- 230000007717 exclusion Effects 0.000 description 2
- 108020001507 fusion proteins Proteins 0.000 description 2
- 102000037865 fusion proteins Human genes 0.000 description 2
- 230000037442 genomic alteration Effects 0.000 description 2
- 229940096919 glycogen Drugs 0.000 description 2
- 210000004458 heterochromatin Anatomy 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- 210000003917 human chromosome Anatomy 0.000 description 2
- 238000010166 immunofluorescence Methods 0.000 description 2
- 238000012623 in vivo measurement Methods 0.000 description 2
- 230000002452 interceptive effect Effects 0.000 description 2
- 239000012139 lysis buffer Substances 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 210000000299 nuclear matrix Anatomy 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 230000002688 persistence Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 230000003252 repetitive effect Effects 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 229960005322 streptomycin Drugs 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 230000002103 transcriptional effect Effects 0.000 description 2
- 241001244729 Apalis Species 0.000 description 1
- 241000208340 Araliaceae Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 102100025570 Cancer/testis antigen 1 Human genes 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010068051 Chimerism Diseases 0.000 description 1
- 238000001353 Chip-sequencing Methods 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 239000004971 Cross linker Substances 0.000 description 1
- CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 1
- 238000000116 DAPI staining Methods 0.000 description 1
- 230000007067 DNA methylation Effects 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 230000007023 DNA restriction-modification system Effects 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 1
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 1
- 101100364969 Dictyostelium discoideum scai gene Proteins 0.000 description 1
- ZFIVKAOQEXOYFY-UHFFFAOYSA-N Diepoxybutane Chemical compound C1OC1C1OC1 ZFIVKAOQEXOYFY-UHFFFAOYSA-N 0.000 description 1
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
- 108010022894 Euchromatin Proteins 0.000 description 1
- 208000034454 F12-related hereditary angioedema with normal C1Inh Diseases 0.000 description 1
- 241000287227 Fringillidae Species 0.000 description 1
- 102100036263 Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial Human genes 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 102100039869 Histone H2B type F-S Human genes 0.000 description 1
- 108010033040 Histones Proteins 0.000 description 1
- 102000006947 Histones Human genes 0.000 description 1
- 101000856237 Homo sapiens Cancer/testis antigen 1 Proteins 0.000 description 1
- 101001001786 Homo sapiens Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial Proteins 0.000 description 1
- 101001035372 Homo sapiens Histone H2B type F-S Proteins 0.000 description 1
- 101000976075 Homo sapiens Insulin Proteins 0.000 description 1
- 241000430519 Human rhinovirus sp. Species 0.000 description 1
- 102000006496 Immunoglobulin Heavy Chains Human genes 0.000 description 1
- 108010019476 Immunoglobulin Heavy Chains Proteins 0.000 description 1
- 108010059724 Micrococcal Nuclease Proteins 0.000 description 1
- 101100364971 Mus musculus Scai gene Proteins 0.000 description 1
- 101500006448 Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) Endonuclease PI-MboI Proteins 0.000 description 1
- 101100384865 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cot-1 gene Proteins 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000021736 acetylation Effects 0.000 description 1
- 238000006640 acetylation reaction Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 208000005980 beta thalassemia Diseases 0.000 description 1
- 230000002457 bidirectional effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000005889 cellular cytotoxicity Effects 0.000 description 1
- 210000003679 cervix uteri Anatomy 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 239000012297 crystallization seed Substances 0.000 description 1
- 229960004397 cyclophosphamide Drugs 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000008846 dynamic interplay Effects 0.000 description 1
- 108010030074 endodeoxyribonuclease MluI Proteins 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 210000004407 endothelial cell of postcapillary venule of lymph node Anatomy 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- 210000000632 euchromatin Anatomy 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000004077 genetic alteration Effects 0.000 description 1
- 231100000118 genetic alteration Toxicity 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 208000016861 hereditary angioedema type 3 Diseases 0.000 description 1
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 description 1
- 238000012482 interaction analysis Methods 0.000 description 1
- 230000009878 intermolecular interaction Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000002350 laparotomy Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 229960004961 mechlorethamine Drugs 0.000 description 1
- HAWPXGHAZFHHAD-UHFFFAOYSA-N mechlorethamine Chemical class ClCCN(C)CCCl HAWPXGHAZFHHAD-UHFFFAOYSA-N 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000021121 meiosis Effects 0.000 description 1
- 229960001924 melphalan Drugs 0.000 description 1
- SGDBTWWWUNNDEQ-LBPRGKRZSA-N melphalan Chemical compound OC(=O)[C@@H](N)CC1=CC=C(N(CCCl)CCCl)C=C1 SGDBTWWWUNNDEQ-LBPRGKRZSA-N 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 229960004857 mitomycin Drugs 0.000 description 1
- 230000000394 mitotic effect Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 238000001956 neutron scattering Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 210000002353 nuclear lamina Anatomy 0.000 description 1
- 239000011824 nuclear material Substances 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 238000002966 oligonucleotide array Methods 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 230000010399 physical interaction Effects 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 208000003580 polydactyly Diseases 0.000 description 1
- 229920005594 polymer fiber Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000011176 pooling Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 229940121649 protein inhibitor Drugs 0.000 description 1
- 239000012268 protein inhibitor Substances 0.000 description 1
- 239000013014 purified material Substances 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 150000005621 tetraalkylammonium salts Chemical class 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 238000009966 trimming Methods 0.000 description 1
- 238000002525 ultrasonication Methods 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- 238000012070 whole genome sequencing analysis Methods 0.000 description 1
- 210000001325 yolk sac Anatomy 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/301—Endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/501—Ligase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2523/00—Reactions characterised by treatment of reaction samples
- C12Q2523/10—Characterised by chemical treatment
- C12Q2523/101—Crosslinking agents, e.g. psoralen
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/133—Detection mode being characterised by the assay principle conformational analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/501—Detection characterised by immobilisation to a surface being an array of oligonucleotides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Bioinformatics & Computational Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1320351.8 | 2013-11-18 | ||
| GBGB1320351.8A GB201320351D0 (en) | 2013-11-18 | 2013-11-18 | Method |
| PCT/IB2014/002485 WO2015071748A1 (en) | 2013-11-18 | 2014-11-18 | Method for analysing the interaction of nucleotide sequences in a three-dimensional dna structure |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20160079120A KR20160079120A (ko) | 2016-07-05 |
| KR102332522B1 true KR102332522B1 (ko) | 2021-11-29 |
Family
ID=49883800
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020167015969A Active KR102332522B1 (ko) | 2013-11-18 | 2014-11-18 | 삼차원 dna 구조에서 뉴클레오티드 서열의 상호작용을 분석하기 위한 방법 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US10934575B2 (enExample) |
| EP (1) | EP3071710B1 (enExample) |
| JP (1) | JP2016537029A (enExample) |
| KR (1) | KR102332522B1 (enExample) |
| CN (1) | CN105992825A (enExample) |
| AU (1) | AU2014349817B2 (enExample) |
| CA (1) | CA2928012C (enExample) |
| GB (1) | GB201320351D0 (enExample) |
| WO (1) | WO2015071748A1 (enExample) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2554572B (en) * | 2015-03-26 | 2021-06-23 | Dovetail Genomics Llc | Physical linkage preservation in DNA storage |
| JP2018518203A (ja) * | 2015-06-24 | 2018-07-12 | オックスフォード バイオダイナミックス リミテッド | エピジェネティックな染色体相互作用 |
| WO2017066908A1 (zh) * | 2015-10-19 | 2017-04-27 | 安诺优达基因科技(北京)有限公司 | 构建高分辨率、大信息量单细胞Hi-C文库的方法 |
| CN115369161A (zh) * | 2015-12-04 | 2022-11-22 | 10X 基因组学有限公司 | 用于核酸分析的方法和组合物 |
| GB201608000D0 (en) * | 2016-05-06 | 2016-06-22 | Oxford Biodynamics Ltd | Chromosome detection |
| EP3455356B1 (en) | 2016-05-13 | 2021-08-04 | Dovetail Genomics LLC | Recovering long-range linkage information from preserved samples |
| CA3045070A1 (en) * | 2016-12-01 | 2018-06-07 | Oxford Biodynamics Limited | Application of epigenetic chromsomal interactions in cancer diagnostics |
| WO2019005763A1 (en) * | 2017-06-26 | 2019-01-03 | Phase Genomics Inc. | METHOD FOR REGROUPING DNA SEQUENCES |
| CN108300767B (zh) * | 2017-10-27 | 2021-08-20 | 清华大学 | 一种核酸复合体中核酸区段相互作用的分析方法 |
| CN108197431B (zh) * | 2018-01-24 | 2022-04-05 | 清华大学 | 染色质相互作用差异的分析方法和系统 |
| US12378592B2 (en) * | 2018-01-31 | 2025-08-05 | Dovetail Genomics, Llc. | Sample prep for DNA linkage recovery |
| EP3847261A1 (en) * | 2018-09-05 | 2021-07-14 | Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-Gemeinschaft | A method for engineering synthetic cis-regulatory dna |
| WO2020234608A1 (en) * | 2019-05-22 | 2020-11-26 | Oxford Nanopore Technologies Limited | Protocol for detecting interactions within one or more dna molecules within a cell |
| GB2607514B (en) | 2020-07-20 | 2023-06-21 | Enanta Pharm Inc | Functionalized peptides as antiviral agents |
| US11384090B2 (en) | 2020-11-23 | 2022-07-12 | Enanta Pharmaceuticals, Inc. | Spiropyrrolidine derived antiviral agents |
| CN116829571A (zh) | 2020-11-23 | 2023-09-29 | 英安塔制药有限公司 | 新型螺旋吡咯烷衍生的抗病毒药物 |
| US11352363B1 (en) | 2020-11-23 | 2022-06-07 | Enanta Pharmaceuticals, Inc. | Spiropyrrolidine derived antiviral agents |
| CN113215141A (zh) * | 2021-02-23 | 2021-08-06 | 华南农业大学 | 细菌hi-c基因组及质粒构象捕获方法 |
| US11319325B1 (en) | 2021-05-11 | 2022-05-03 | Enanta Pharmaceuticals, Inc. | Macrocyclic spiropyrrolidine derived antiviral agents |
| US11325916B1 (en) | 2021-07-29 | 2022-05-10 | Enanta Pharmaceuticals, Inc. | Spiropyrrolidine derived antiviral agents |
| US11339170B1 (en) | 2021-07-23 | 2022-05-24 | Enanta Pharmaceuticals, Inc. | Spiropyrrolidine derived antiviral agents |
| GB202111195D0 (en) * | 2021-08-03 | 2021-09-15 | Cergentis B V | Method for targeted sequencing |
| CN113946730B (zh) * | 2021-10-19 | 2023-03-17 | 四川大学 | 一种基于基因数据的染色质层次结构分析的可视化方法 |
| US20240095226A1 (en) * | 2022-09-15 | 2024-03-21 | Rodney Kuhn Haffnerson King | Methods and related devices for storing and accessing data using multi-level fractal grids |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007004057A2 (en) * | 2005-07-04 | 2007-01-11 | Erasmus University Medical Center | Chromosome conformation capture-on-chip (4c) assay |
| WO2008084405A2 (en) | 2007-01-11 | 2008-07-17 | Erasmus University Medical Center | Circular chromosome conformation capture (4c) |
| WO2012005595A2 (en) | 2010-07-09 | 2012-01-12 | Wouter Leonard De Laat | V3-d genomic region of interest sequencing strategies |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7348178B1 (en) * | 1994-03-14 | 2008-03-25 | New York University Medical Center | Recombinant adenoviral vector system |
| US10287621B2 (en) * | 2013-04-11 | 2019-05-14 | Pelin Sahlén | Targeted chromosome conformation capture |
-
2013
- 2013-11-18 GB GBGB1320351.8A patent/GB201320351D0/en not_active Ceased
-
2014
- 2014-11-18 CA CA2928012A patent/CA2928012C/en active Active
- 2014-11-18 EP EP14815428.9A patent/EP3071710B1/en active Active
- 2014-11-18 CN CN201480062775.XA patent/CN105992825A/zh active Pending
- 2014-11-18 WO PCT/IB2014/002485 patent/WO2015071748A1/en not_active Ceased
- 2014-11-18 JP JP2016553748A patent/JP2016537029A/ja active Pending
- 2014-11-18 KR KR1020167015969A patent/KR102332522B1/ko active Active
- 2014-11-18 US US15/037,210 patent/US10934575B2/en active Active
- 2014-11-18 AU AU2014349817A patent/AU2014349817B2/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007004057A2 (en) * | 2005-07-04 | 2007-01-11 | Erasmus University Medical Center | Chromosome conformation capture-on-chip (4c) assay |
| JP2009500031A (ja) | 2005-07-04 | 2009-01-08 | エラスムス ユニバーシティ メディカル センター | 染色体コンフォメーションキャプチャ−オン−チップ(4c)アッセイ |
| WO2008084405A2 (en) | 2007-01-11 | 2008-07-17 | Erasmus University Medical Center | Circular chromosome conformation capture (4c) |
| JP2010515449A (ja) | 2007-01-11 | 2010-05-13 | エラスムス ユニバーシティ メディカル センター | 環状染色体コンホメーション捕捉(4c) |
| WO2012005595A2 (en) | 2010-07-09 | 2012-01-12 | Wouter Leonard De Laat | V3-d genomic region of interest sequencing strategies |
| JP2013530709A (ja) | 2010-07-09 | 2013-08-01 | セルゲンティス ビー.ブイ. | 対象の3−dゲノム領域配列決定戦略 |
Non-Patent Citations (1)
| Title |
|---|
| Nature Genetics volume 38, pages 1348-1354 (2006. 11.) |
Also Published As
| Publication number | Publication date |
|---|---|
| EP3071710A1 (en) | 2016-09-28 |
| AU2014349817B2 (en) | 2020-11-12 |
| WO2015071748A1 (en) | 2015-05-21 |
| CA2928012A1 (en) | 2015-05-21 |
| GB201320351D0 (en) | 2014-01-01 |
| CN105992825A (zh) | 2016-10-05 |
| US10934575B2 (en) | 2021-03-02 |
| CA2928012C (en) | 2023-05-16 |
| AU2014349817A1 (en) | 2016-05-19 |
| US20160289738A1 (en) | 2016-10-06 |
| KR20160079120A (ko) | 2016-07-05 |
| EP3071710B1 (en) | 2019-05-15 |
| JP2016537029A (ja) | 2016-12-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR102332522B1 (ko) | 삼차원 dna 구조에서 뉴클레오티드 서열의 상호작용을 분석하기 위한 방법 | |
| AU2021204024B2 (en) | RNA-guided human genome engineering | |
| EP2083090B1 (en) | Nucleic acid interaction analysis | |
| EP3365464B1 (en) | Method of analysing dna sequences | |
| CN106566828B (zh) | 一种高效的全基因组染色质构象技术eHi-C | |
| US11370810B2 (en) | Methods and compositions for preparing nucleic acids that preserve spatial-proximal contiguity information | |
| US20190187156A1 (en) | Methods for identifying macromolecule interactions | |
| US20220033807A1 (en) | Method for capturing rna in situ higher-order structures and interactions | |
| HK1228460B (en) | Method for analysing the interaction of nucleotide sequences in a three-dimensional dna structure | |
| HK1228460A1 (en) | Method for analysing the interaction of nucleotide sequences in a three-dimensional dna structure | |
| Knoch | A Guided Protocol for Array Based T2C: A High‐Quality Selective High‐Resolution High‐Throughput Chromosome Interaction Capture | |
| HK40116830A (en) | Rna-guided human genome engineering | |
| Franke | The role of higher-order chromatin organization at the SOX9 locus in gene regulation and disease | |
| HK40103974A (en) | Rna-guided human genome engineering | |
| HK40014881B (en) | Rna-guided human genome engineering | |
| HK40014881A (en) | Rna-guided human genome engineering | |
| HK1212376B (en) | Rna-guided human genome engineering |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0105 | International application |
Patent event date: 20160615 Patent event code: PA01051R01D Comment text: International Patent Application |
|
| PG1501 | Laying open of application | ||
| A201 | Request for examination | ||
| PA0201 | Request for examination |
Patent event code: PA02012R01D Patent event date: 20191112 Comment text: Request for Examination of Application |
|
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20201218 Patent event code: PE09021S01D |
|
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20210715 Patent event code: PE09021S01D |
|
| E701 | Decision to grant or registration of patent right | ||
| PE0701 | Decision of registration |
Patent event code: PE07011S01D Comment text: Decision to Grant Registration Patent event date: 20210902 |
|
| GRNT | Written decision to grant | ||
| PR0701 | Registration of establishment |
Comment text: Registration of Establishment Patent event date: 20211124 Patent event code: PR07011E01D |
|
| PR1002 | Payment of registration fee |
Payment date: 20211124 End annual number: 3 Start annual number: 1 |
|
| PG1601 | Publication of registration |