KR102323471B1 - Decanting-kit container for divide extraction easy by ingredient specific - Google Patents

Abstract

The present invention relates to a decanting kit that can be easily separated and extracted for each component, and more particularly, in the separation of blood or bone marrow components using a centrifuge, selective extraction of the concentrated and separated components according to the density difference is easy. It relates to a decanting kit that is easy to separate and extract each component,
When centrifuging using a centrifuge, a centrifugal separation vessel is formed so that the sample contained therein is concentrated and separated for each component by the density difference; a decanting vessel attached to the upper side of the centrifuge vessel and decanting the components separated from the centrifuge vessel in a state of being inclined at a certain inclination; a floater provided inside the centrifugation vessel, moving along the longitudinal direction of the centrifugation vessel, and positioned at the boundary between the centrifuged components by the difference in density of each component of the sample to be centrifuged; and an insert cover seated inside the centrifugation vessel to limit the movement distance of the floater moving in the longitudinal direction of the centrifugation vessel; provides a decanting kit for easy separation and extraction by component, comprising: .

Description

A decanting kit for easy separation and extraction by component {DECANTING-KIT CONTAINER FOR DIVIDE EXTRACTION EASY BY INGREDIENT SPECIFIC}

The present invention relates to a decanting kit that can be easily separated and extracted for each component, and more particularly, in the separation of blood or bone marrow components using a centrifuge, the selective extraction of the concentrated and separated components according to the density difference is easy. It relates to a decanting kit that is easy to separate and extract each component.

When a suspension with a floating material is left still, the dense material slowly sinks to the bottom under the influence of gravity and the low density material slowly moves to the upper layer. It is called (sediment, 沈澱).

As such, when substances with different densities are mixed, a precipitation phenomenon occurs, and over time, the mixture can be separated according to the density difference. That is, the density difference between mixtures increases as gravity, the force that separates the mixtures, increases, so artificially increasing the gravity can accelerate the sedimentation phenomenon.

Therefore, if centrifugal force is used instead of gravity, sedimentation can be easily accelerated. This process is called centrifugation. A centrifugal separator (遠心分離機) is a device used to separate, purify, and concentrate substances with different components or specific gravity (density difference) using the principle of centrifugation described above. , can be separated for wastewater treatment, uranium enrichment, production, and experimental use.

Among them, the medical centrifuge is used not only for the purpose of separating components for analysis of blood, urine, saliva, etc., but also for the purpose of extracting platelet-rich plasma. Here, the platelet-rich plasma (PRP) can be obtained by centrifugation of a complex fluid such as bone marrow or blood (hereinafter referred to as "sample"), and is highly enriched with platelets more than normal bone marrow or blood. , 高濃縮) refers to plasma components, and contains several growth factors, so it works to heal wounds and regenerate damaged areas. For example, it is used for the purpose of reconstructing the damaged part by injecting it into the damaged area such as ligament (靭帶) and cartilage.

On the other hand, the centrifuge described above is divided into various types according to the amount of the sample to be centrifuged, the rotational speed, the type of the rotor, etc., and the components of the sample are separated and recovered by the centrifugal force of the rotor rotation It includes a centrifuge vessel for

Based on this technical idea, through Korean Patent Publication No. 10-2007-0026316, a disposable container for centrifuging and processing a fluid biological material has been disclosed, but a specific component among the centrifuged components in the container In the selective extraction of bay, the centrifuged ingredients based on the process of sucking and extracting ingredients by inserting a syringe needle into the third canola (hose) simply provided so as to extend from the cover to the bottom of the container ( chamber), due to the change in the sample content in the container (reduction of the fresh water level of the sample) during suction extraction (suction) using the syringe, a phenomenon in which the centrifuged components are mixed again is induced, and extraction is performed in that state There was a limit to perfectly extracting only specific ingredients.

In addition, since an adhesive has been used for bonding between the cover and the container, there is a problem in that it is not easy to assemble and manufacture the centrifuge container, and in addition, since the sample injection port and the extraction port are simply formed of a rubber material, the injection needle is accurately positioned There has been a problem in that it is not easy to inject a sample or to separate and extract the separated components in the state of being infiltrated into the .

(Document 1) Registered Patent Publication No. 10-1894966 (Registered on August 29, 2018) (Document 2) Registered Patent Publication No. 10-1197974 (Registered on October 30, 2012) (Document 3) Registered Patent Publication No. 10-1874548 (Registered on June 28, 2018)

An object of the present invention is to solve the above problems, and in separating a sample using the centrifugal force according to the rotor rotation by the operation of the centrifuge and the difference in specific gravity for each component, the component using the density difference for each component It is to provide a decanting kit that is easy to separate and extract each component so that the separation can be accurate and the extraction of the characteristic component among the separated components can be performed perfectly, easily and quickly.

Another object of the present invention is to form a centrifuge container for concentration and separation of each component of a sample and a component recovery container in which a specific component having a low density among the concentrated and separated components is separated and stored in a structure that can be disassembled and assembled with each other, It is to provide a decanting kit that is easy to separate and extract each component so that the work efficiency of extracting specific components as well as centrifugation of the sample as well as the centrifugation of the sample due to easy storage along with concentration separation and selective extraction recovery can be greatly improved.

In order to achieve the above object, the present invention provides a centrifugal separation vessel that is formed so that, during centrifugation using a centrifuge, a sample contained therein is concentrated and separated for each component by a density difference; a decanting vessel attached to the upper side of the centrifuge vessel and decanting the components separated from the centrifuge vessel in a state of being inclined at a certain inclination; a floater provided inside the centrifugation vessel, moving along the longitudinal direction of the centrifugation vessel, and positioned at the boundary between the centrifuged components by the difference in density of each component of the sample to be centrifuged; and an insert cover seated inside the centrifugation vessel to limit the movement distance of the floater moving along the longitudinal direction of the centrifuge vessel .

In the present invention, the centrifuge container includes a centrifugal separation chamber in which a predetermined amount of a sample to be centrifuged is stored and a centrifugal separation chamber is formed inside so that the sample is concentrated and separated by component during centrifugation, and an upper opening of the centrifugal separation container body. a centrifugal container cover provided with an injection port for sample injection into the centrifuge container, a ventilation port through which air is discharged during sample injection, and a discharge unit through which centrifuged components are discharged, and the centrifugal separation container body; and a first packing ring interposed between the centrifugal container covers to seal the inside, wherein the centrifugal separation body includes a flange formed from the upper end of the body to the outside to which the centrifugal container cover is hooked, and the flange. A ring step formed on the inner side of the centrifugal container cover and a first packing ring for sealing the main body is seated, and a coupling groove formed inside the ring step to form the same inner circumferential surface as the inside of the centrifuge container cover The centrifugal separation container cover includes a centrifugal separation container cover body having a cover side hook-coupled to the outer circumferential surface of the flange, and is formed inside the cover side surface to seat the flange and the first packing ring A ring seating groove, a coupling step inserted into the coupling groove to form an inner circumferential surface such as the inner circumferential surface of the main body for centrifugation, and decanting while the centrifuged sample forms a guide surface equal to the inner circumferential surface of the centrifugal separation body for a predetermined period It is characterized in that it consists of a discharge part protruding toward the upper side so that it can be decanted to the container side.

In the present invention, the decanting container includes a main body for decanting having a decanting chamber formed therein so that the components discharged from the centrifugal separation container are separated and moved and stored, and an upper end of the opening of the main body for decanting is sealed, but the extraction of components from the main body for decanting is performed. It is composed of a decanting container cover having a discharge port and a ventilation port formed therein to enable it, and on one side of the main body for decanting, such as the discharge unit so that the low-density component discharged from the discharge unit of the centrifugal separation body can flow. An inlet portion having a guide surface is formed, and the outlet portion includes an upper flange of a certain shape formed by extending an upper portion by a certain distance from the cover body, and a cover coupling step that extends from the upper flange to the upper side to form a step inward, Consists of an outlet of a certain shape formed inside the cover coupling step, the inlet portion is seated on the cover coupling step of the outlet and has an inlet having the same guide surface as the outlet, the outside of the cover coupling step and a second packing ring and a coupling hook of a certain shape that surrounds the upper flange and is hook-coupled to the upper flange, and is interposed between the outside of the cover coupling step and the coupling hook and consists of a second packing ring for sealing the discharge part and the inlet part. do.

The decanting kit, which facilitates separation and extraction by component according to the present invention, concentrates and separates the components of the sample by component by a centrifugal separation vessel and separates and stores the specific components using the component recovery vessel, so that the component separation process of the sample And it brings the effect of making the selective extraction process of specific components easier.

In addition, since the present invention can prevent unnecessary components from being included in the extraction components by limiting the movement distance of the floater moving up and down in the centrifugal separation vessel, only a specific component is perfectly extracted.

In addition, the present invention can separate only a desired component from blood or bone marrow used for autologous bone marrow therapy and autologous blood therapy, and achieve medical results such as enhancing the therapeutic effect using the separated component (platelet-rich plasma, etc.). Of course, it is a very useful invention that can greatly contribute to the development of the related medical industry.

1 is a perspective view of a decanting kit that is easy to separate and extract each component according to the present invention.
Figure 2 is an exploded perspective view of the centrifugal container of the decanting kit for easy separation and extraction by component according to the present invention.
Figure 3 is a bottom perspective view of the centrifuge vessel cover according to the present invention.
Figure 4 is an exploded perspective view of the decanting container of the decanting kit for easy separation and extraction by component according to the present invention.
5 is a perspective view from the bottom side of the decanting container according to the present invention.
6 is a cross-sectional view of a decanting kit that is easy to separate and extract each component according to the present invention.
7 is an operation diagram of the decanting kit for easy separation and extraction by component according to the present invention.
8 and 9 are exemplary views illustrating the operation of the centrifuge rotor of the decanting kit for easy separation and extraction by component according to the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those of ordinary skill in the art can easily practice the present invention. First, in adding reference numerals to the components of the drawings, it should be noted that the same components are given the same reference numerals as much as possible even though they are indicated on different drawings. In addition, in describing the present invention, if it is determined that a detailed description of a related known configuration or function may obscure the gist of the present invention, the detailed description thereof will be omitted.

1 is a perspective view of a decanting kit for easy separation and extraction for each component according to the present invention, FIG. 2 is an exploded perspective view of a centrifugal container of a decanting kit for easy separation and extraction for each component according to the present invention, FIG. 4 is an exploded perspective view of the decanting container of the decanting kit that is easy to separate and extract each component according to the present invention, and FIG. 5 is a perspective view from the bottom side of the decanting container according to the present invention. 6 is a cross-sectional view of the decanting kit for easy separation and extraction by component according to the present invention, FIG. 7 is an operational diagram of the decanting kit for easy separation and extraction for each component according to the present invention, and FIGS. 8 and 9 are It is an exemplary view illustrating the operation of the centrifuge rotor of the decanting kit that is easy to separate and extract each component according to the present invention.

As shown in FIG. 1, the decanting kit 1 for easy separation and extraction of each component according to the present invention is a centrifugal separation container in which the sample contained therein is concentrated and separated by component due to the density difference during centrifugation using a centrifuge. (10) and the centrifugal container 10, which is connected to the upper part of one side, and the decanting container 20 in which the components flowing out from the centrifugal container 10 that are in a state inclined to the horizontal by the centrifugal force during centrifugation are separated and stored is comprised of

The centrifuge 3 will be described with reference to FIG. 8 . The centrifuge 3 is a device used for the purpose of separating and recovering only specific components from a complex fluid (hereinafter referred to as "sample"), such as separating blood cells and plasma from blood or stem cells from bone marrow, 8 and 9, a rotor 2 that rotates horizontally when the device is driven is installed therein, and a plurality of rotating together when the rotor 2 rotates in a state in which the decanting kit 1 is accommodated and mounted. A bucket (2-1) is provided.

In addition, on one side of the periphery of the bucket 2-1, as shown in FIG. 9, a hole 2-2 for looking into the inside of the decanting kit 1 or reducing the load on the back is formed.

The decanting kit 1 serves as a storage medium for concentration and separation of each component of the sample, and can contain a predetermined amount of a sample to be subjected to centrifugation, and a centrifugation chamber 14 in which the sample is concentrated and separated by component during centrifugation. ) provided with the centrifugal separation container body 11, the centrifugal separation container cover 12 sealing the upper opening of the centrifugal separation body 11 but allowing sample injection into the centrifugation chamber 14, and the centrifugal separation container body ( 11) The floater 13 provided inside and moving along the longitudinal direction of the centrifugal separation container body 11 so as to be located at the boundary of each component based on the difference in density of each component of the sample to be centrifuged, and the upper and lower sides of the floater 13 It consists of an insert cover 15 that is seated in the inner centrifugation chamber 14 of the centrifugal separation chamber main body 11 to limit movement.

As shown in FIG. 2 , the centrifugal separation vessel 10 is formed from the upper end of the centrifugal separation vessel body 11 and the centrifugal separation vessel body 11 to the outside, and the centrifugal separation vessel cover 12 is hook-coupled. A ring step 1113 formed on the inside of the flange 114 and the flange 114 and on which the first packing ring 30a for sealing the centrifuge container cover 12 and the centrifugal separation main body 11 is seated and , is formed on the inside of the ring step 113 and is composed of a coupling groove 112 for forming the same inner circumferential surface as the inside of the centrifuge container cover 12, and the upper end 111 of the centrifugal separation container body 11 is a ring step (113) together with a coupling groove (112). In addition, a fastening fixing groove 116 for fixing the coupling position of the centrifugal container cover 12 is formed on one side of the flange 114 . The fastening fixing groove 116 can fix the mounting position of the cover 12 while the fastening fixing protrusions 121-2 formed on the centrifugal container cover 12 are inserted.

As shown in Figures 2 and 3, the centrifugal separation container cover 12 is a centrifugal separation container cover body 121 provided with a cover side surface 121-1 hooked to the outer circumferential surface of the flange 114, and the cover It is formed on the inside of the side surface 121-1 and is inserted into the ring seating groove 129 in which the flange 114 and the first packing ring 30a are seated, and the coupling groove 112, such as the inner peripheral surface of the main body for centrifugation. A coupling step 127 for forming an inner circumferential surface and an inner circumferential surface equal to the inner circumferential surface of the centrifugal separation container body 11 are formed to protrude upward so that the centrifuged component can be decanted toward the decanting container 20 side. It consists of part 125 . The discharge unit 125 is a guide plate 125-1 forming the same inner surface as the inner circumferential surface of the main body 11 for centrifugation, and a discharge port 125-2 having a predetermined shape formed by the guide plate 125-1. ), an upper flange 125-3 extending upward from the centrifugal container cover body 121 by a certain distance, and a cover extending from the upper flange 125-3 to the upper part by a certain distance to form a step inward It is composed of a coupling step (125-4). The guide plate 125-1 is formed on the same inner circumferential surface as the inner circumferential surface of the centrifugal separation vessel main body 11 to form a flow path so that the centrifuged specific sample can easily move to the decanting vessel 20. The centrifuge container cover 12 is provided with an injection port 126 and a ventilation port 122, the sample is injected into the chamber 14 of the centrifuge vessel through the injection port 126, and through the injection port 126 When the sample is injected, the air may be discharged to the outside through the ventilation port 122 .

4 and 5 show the decanting vessel 20 . The decanting container 20 seals the opening upper end of the decanting main body 21 and the decanting main body 21 in which the decanting chamber 23 is formed so that the components discharged from the centrifugal separation container 10 are separated and moved and stored. However, it is composed of a decanting container cover 22 in which an exhaust port 221 and a ventilation port 222 are formed to enable extraction of components from the decanting main body 21 . A decanting chamber 213 and an inlet 211 are provided in the main body 21 for decanting. The inlet 211 includes an outer guide plate 211-1 hooked to the upper flange 125-3, an inner guide plate 211-2 formed in the same manner as the guide plate 125-1, and an outer guide plate ( 211-1) and an inlet coupling groove 211-4 formed by the inner guide plate 211-2, and an inlet 211-3 formed by the inner guide plate 211-2. The inner guide plate 211-2 is seated on the cover coupling step 125-4, and a second packing ring 30b is interposed between the outer surface of the cover coupling step 125-4 and the outer guide plate 211-1. do. A barrier 214 is formed at the portion entering the decanting chamber 213 through the inlet 211-3 to allow the centrifuged component to smoothly move into the decanting chamber 213 . The decanting chamber 213 is formed by an inclined surface 218 downwardly formed at a predetermined angle on both sides and a storage groove 219 in which the centrifuged component passing through the inclined surface 218 is stored. The upper end of the decanting container 21 is provided with an extended end 216 extending upward by a predetermined length, and a decanting flange 217 formed on the outer surface of the extended end.

The decanting container cover 22 includes a decanting container cover body 221 , an outer surface portion 223 extending downward from the decanting container cover body 221 to form an edge, and a decanting container cover body 221 formed on the body 221 . It consists of an extraction port 222 and a ventilation port 224 . The outer surface portion 223 of the decanting container cover body is hook-coupled to the decanting flange 217. Between the outer surface portion 223 coupled to the decanting flange 217 and the extension end 216, a third packing ring for sealing ( 30c) is interposed.

The floater (13) is to prevent the sedimentation component and the suspended component from mixing with each other through the boundary of each component of the sample concentrated and separated by component inside the centrifugal separation vessel body 11, as shown in FIG. , the floater 13 may be configured in the form of a basket or plate. A plurality of footrests 132 are installed at the bottom of the floater to facilitate the sedimentation of high-density components into the lower side of the centrifugation chamber 14 during centrifugation by forming a sufficient gap with the bottom plate of the centrifugal separation container body 11 and the floater. It is protruded from the bottom of the plate, and a through hole 131 is formed in the center of the bottom plate of the floater so that a component with a high density passes through and settles on the bottom of the bottom plate during centrifugation.

In addition, in the central part of the upper surface of the bottom plate through the through hole 131, a barrier 133 that blocks the reverse flow of the sedimentation component while easy precipitation of the component through the through hole 131 is protruding, and at the upper end of the through hole 131 An expanded pipe portion that facilitates the discharge of components having a high density may be further formed.

The operation of the decanting kit 1 for easy separation and extraction for each component according to the present invention having the above configuration will be described in detail as follows.

First, the sample contained in the syringe is transferred to the centrifuge container 10 for separation by component of a sample such as blood or bone marrow collected from a patient using a syringe.

In more detail, it is injected into the centrifugal separation chamber 14 in the centrifugal separation vessel main body 11 through the injection port portion 126 formed on the cover 12 of the centrifugal separation vessel 10 .

When the sample is injected into the centrifuge main body 11, the air remaining in the centrifugation chamber 14 is discharged to the outside through the ventilation port 122 formed in the centrifuge container cover 12, so the centrifugal separation main body The internal pressure of (11) is kept constant.

Next, as shown in FIGS. 8 to 9, the decanting kit 1 of the present invention is accommodated in each bucket 2-1 hinged to the rotor 2 of the centrifuge 3, and then centrifuged. By driving the separator (3) to rotate the rotor (2) horizontally, the components of the sample contained in the centrifugal separation chamber (14) of the centrifugal separation container body (11) are ordered from low to high density by centrifugal force. are separated

For example, in the case of blood, blood cells, which are components of high density, are concentrated and separated in the lower part of the plotter 13 with respect to the plotter 13 in the centrifugation chamber 14 of the main body 11 for centrifugation, and the plotter 13 . Plasma with low density is concentrated and separated at the upper part of the The floater 13 is moved along the longitudinal direction by receiving a buoyancy force by components having different densities.

During the separation of the sample by component in this process, the floater 13 moves (float) along the longitudinal direction of the centrifugal separation vessel main body 11 due to the density difference of each component and is located at the boundary between the high-density component and the low-density component will do However, the plotter 13 is restricted from moving in the longitudinal direction by the insert cover 15 , and the presence of the insert cover 15 allows the plotter 13 to separate only a certain amount of components, so that it is separated. can increase the efficiency of

As the rotor 2 rotates according to the operation of the centrifuge 3 as described above, the sample in the centrifuge container 11 is centrifuged, and when the operation of the centrifuge stops, a separate angle maintaining means (not shown) The centrifugal separation vessel 11 is operated to be inclined at a predetermined angle together with the bucket 2-1 by the ) flows into When all the components of the upper part of the floater 13 flow into the decanting container 20, the angle maintaining means returns the bucket 2-1 to its original state, and the decanting is finished.

Next, the separated components are extracted from the decanting container 20 of the decanting kit 1, and in order to extract the separated components, the extracted components are extracted with a syringe through the extraction port 222.

The above description is merely illustrative of the technical idea of the present invention, and those of ordinary skill in the art to which the present invention pertains within the scope not departing from the essential characteristics of the present invention as shown in , various modifications, changes and substitutions will be possible. Accordingly, the embodiments disclosed in the present invention and the accompanying drawings are intended to explain, not to limit the technical spirit of the present invention, and the scope of the technical spirit of the present invention is not limited by these embodiments and the accompanying drawings. . The protection scope of the present invention should be construed by the following claims, and all technical ideas within the equivalent range should be construed as being included in the scope of the present invention.

1: Decanting Kit 2: Rotor
3: centrifuge
10: centrifugal separation vessel 11: centrifugal separation vessel body
12: centrifuge container cover 13: plotter
14: centrifugal separation chamber 15: insert cover
20: decanting container 21: main body for decanting
22: Decanting container cover
30 (30a, 30b, 30c): packing ring

Claims (3)

When centrifuging using a centrifuge, a centrifugal separation vessel is formed so that the sample contained therein is concentrated and separated for each component by the density difference;
a decanting vessel attached to the upper side of the centrifuge vessel and decanting the components separated from the centrifuge vessel in a state of being inclined at a certain inclination;
a floater provided inside the centrifugation vessel, moving along the longitudinal direction of the centrifugation vessel, and positioned at the boundary between the centrifuged components by the difference in density of each component of the sample to be centrifuged; and
and an insert cover seated inside the centrifugation vessel to limit the movement distance of the floater moving along the longitudinal direction of the centrifuge vessel;
The centrifugation vessel includes a centrifugal separation vessel body in which a predetermined amount of a sample to be centrifuged is stored and a centrifugation chamber is formed inside so that the sample is concentrated and separated by component during centrifugation, and an upper opening of the centrifugal separation vessel body is sealed. However, the centrifugal separation vessel cover provided with an injection port for injecting a sample into the centrifugal separation vessel, a ventilation port through which air is discharged during sample injection, and a discharge unit through which the centrifuged component is discharged, and the centrifugal separation vessel body and the centrifuge It is interposed between the separation container covers and consists of a first packing ring for sealing the inside,
The main body for centrifugation includes a flange formed from the upper end of the main body to the outside to which the centrifuge container cover is hooked, and a first packing ring formed inside the flange for sealing the centrifugal container cover and the body is seated. It is formed as a ring step, and a coupling groove formed on the inside of the ring step to form the same inner circumferential surface as the inside of the centrifugal container cover,
The centrifugal separation container cover includes a centrifugal separation container cover body having a cover side hook-coupled to the outer circumferential surface of the flange, and a ring seating groove formed inside the cover side surface to seat the flange and the first packing ring; , a coupling step inserted into the coupling groove to form an inner circumferential surface such as the inner circumferential surface of the main body for centrifugation, and the centrifuged sample to be decanted toward the decanting vessel while forming a guide surface equal to the inner circumferential surface of the centrifugal separation body for a predetermined period It consists of a discharge part protruding toward the upper side so that
The decanting container includes a main body for decanting having a decanting chamber formed therein so that the components discharged from the centrifugal separation container are separated and moved and stored, and an upper end of the opening of the main body for decanting is sealed, but component extraction from the main body for decanting is possible It is composed of a decanting container cover having a discharge port and a ventilation port to
At one side of the main body for decanting, an inlet having the same guide surface as the outlet is formed so that the low-density component discharged from the outlet of the main body for centrifugation can flow,
The discharge part, an upper flange of a certain shape formed by extending upward a certain distance from the cover body, a cover coupling step extending upward from the upper flange to form a step inwardly, and the cover coupling step formed inside the cover It consists of an outlet of a certain shape,
The inlet is seated on the cover coupling step of the outlet and has an inlet having the same guide surface as the outlet, and a coupling hook of a certain shape that surrounds the outside of the cover coupling step and the second packing ring and is hooked to the upper flange. under,
A decanting kit for easy separation and extraction by component, characterized in that it comprises a second packing ring interposed between the outside of the cover coupling step and the coupling hook to seal the discharge part and the inlet part. delete delete

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