KR20210053668A - Decanting-kit container for divide extraction easy by ingredient specific - Google Patents

Abstract

The present invention relates to a decanting kit capable of easy separation and extraction of an individual ingredient. Particularly, the decanting kit capable of easy separation and extraction of an individual ingredient facilitates selective extraction of an ingredient concentrated and separated depending on a difference in density upon the separation of indegrates of the blood or bone marrow using a centrifugal separator. The decanting kit according to the present invention includes: a centrifugal separation container formed to concentrate and separate a sample received therein into individual ingredients depending on a difference in density upon the centrifugal separation using the centrifugal separator; a decanting container linked to and mounted at the top of the centrifugal separation container, and configured to decant an ingredient separated from the centrifugal separation container in a tilted state with a predetermined slope; a floater provided inside of the centrifugal separation container, moving along the longitudinal direction of the centrifugal separation container, and disposed at the boundary of the ingredients separated centrifugally by the difference in density of the ingredients of the sample subjected to centrifugal separation; and an insert cover mounted inside of the centrifugal separation container and configured to limit the moving distance of the floater moving along the longitudinal direction of the centrifugal separation container.

Description

DECANTING-KIT CONTAINER FOR DIVIDE EXTRACTION EASY BY INGREDIENT SPECIFIC}

The present invention relates to a decanting kit that is easy to separate and extract each component, and more particularly, in the separation of components of blood or bone marrow using a centrifuge, it is possible to facilitate selective extraction of the concentrated and separated components according to the difference in density. It relates to a decanting kit that is easy to separate and extract each component.

If a suspension with floating material is left still, the high density material gradually sinks to the bottom under the influence of gravity, and the low density material gradually moves to the upper layer. This process is precipitated. It is called (sediment, 沈澱).

As such, when a substance having a difference in density is mixed, a precipitation phenomenon occurs, and over time, the mixture can be separated according to the difference in density. That is, the difference in density between the mixtures increases as the gravity that separates the mixtures increases, so if the gravity is artificially increased, the sedimentation phenomenon can be accelerated.

Therefore, if centrifugal force is used instead of gravity, the sedimentation phenomenon can be easily accelerated. This process is called centrifugation (遠心分離). A related centrifugal separator (centrifugal separator) is a device used for separating, purifying, and concentrating substances with different components or specific gravity (density difference) using the above-described principle of centrifugation. It can be separated for wastewater treatment, uranium enrichment, production, and experiment.

Among them, the medical centrifuge is also used for the purpose of separating components for analysis of blood, urine, and saliva, but also for the purpose of extracting platelet-rich plasma. Here, the platelet-rich plasma (PRP) can be obtained by centrifugation of a complex fluid such as bone marrow or blood (hereinafter referred to as "sample"), and is highly enriched in platelets than normal bone marrow or blood. , 高濃縮) Means a plasma component, it contains several growth factors, so it works to heal wounds and regenerate damaged areas. For example, it is used for the purpose of rebuilding the damaged part by injecting it into the damaged part of ligaments and cartilage, and since it uses its own bone marrow or blood, there is no side effect and the treatment effect is fast.

Meanwhile, the centrifugal separator described above is divided into various types according to the amount of the sample to be centrifuged, the rotational speed, the type of the rotor, etc., and the components of the sample are separated and recovered by the centrifugal force of the rotor rotational motion. It includes a centrifuge container for

Based on this technical idea, although a disposable container for centrifuging and processing fluid biological materials has been disclosed through Korean Patent Publication No. 10-2007-0026316, specific components among the components centrifuged in the container In selective extraction of bays, the components centrifuged in accordance with the process of inhaling and extracting the components by inserting a injection needle into a simple provided third canola (hose) extending from the cover to the bottom of the container are placed in a space inside the container ( chamber), a phenomenon in which the centrifuged components are mixed again due to the change in the sample content (lower the sample fresh water level) in the container during suction extraction (suction) using the syringe, and extraction in that state Because there was no choice but to be, there was a limit to extracting only certain ingredients completely.

In addition, there is a problem that it is not easy to assemble and manufacture the centrifugal container because adhesives have been used when bonding the cover and the container. In addition, since the sample injection port and the extraction port are simply formed of rubber material, the injection needle is positioned accurately. There has been a problem in that it is not easy to inject a sample or to separate and extract separated components in a state in which it has penetrated.

(Document 1) Registered Patent Publication No. 10-1894966 (registered on August 29, 2018) (Document 2) Registered Patent Publication No. 10-1197974 (registered on October 30, 2012) (Document 3) Registered Patent Publication No. 10-1874548 (registered on June 28, 2018)

An object of the present invention is to solve the above problems, and in separating a sample by using the difference between the centrifugal force and the specific gravity of each component according to the rotation of the rotor by the operation of the centrifugal separator, the component using the difference in density of each component The aim is to provide a decanting kit that facilitates separation and extraction of each component that enables accurate separation of the components and allows the extraction of characteristic components from the separated components to be performed perfectly, easily and quickly.

Another object of the present invention is to have a structure capable of mutual decomposition and assembly of a centrifuge container for concentration and separation of each component of a sample and a component recovery container in which a specific component having a low density among the concentrated and separated components is separated and stored. The purpose of this is to provide a decanting kit that facilitates separation and extraction of each component that can greatly improve the work efficiency of extracting specific components, as well as centrifugation of the sample, as it facilitates storage as well as concentration separation and selective extraction recovery.

In order to achieve the above object, the present invention provides a centrifugal separator formed such that, when centrifugation is performed using a centrifuge, a sample contained therein is concentrated and separated for each component by a density difference; A decanting container which is connectedly mounted on the upper side of the centrifugal separator and in which the components separated from the centrifugal separator are decanted while inclined at a certain inclination; A plotter provided inside the centrifuge container, moving along the longitudinal direction of the centrifuge container, and positioned at the boundary of the centrifuged component due to the difference in density of each component of the centrifuged sample; And it provides a decanting kit for easy separation and extraction of each component comprising; and an insert cover for limiting the movement distance of the floater that is seated in the centrifugal separator and moves along the longitudinal direction of the centrifugal separator. .

In the present invention, the centrifugal separation vessel includes a centrifugation base body in which a centrifugation chamber is formed inside so that a certain amount of a sample to be centrifuged is stored and the sample is concentrated and separated for each component during centrifugation, and an upper opening of the centrifugal separation body. And a centrifugal separator cover provided with an injection port for injecting a sample into the centrifugal separator, a vent port for discharging air when injecting a sample, and a discharge part for discharging the centrifuged components; and It is interposed between the centrifugation container cover and consists of a first packing ring for sealing the inside, the main body for centrifugation is formed outward from the upper end of the body, a flange to which the centrifugal separator cover is hooked, and the flange A ring step on which the first packing ring for sealing the centrifugation container cover and the main body is seated, and a coupling groove formed on the inside of the ring step to form the same inner circumferential surface as the inside of the centrifugation container cover And the centrifugal separation container cover has a centrifugal separation container cover body provided with a cover side that is hooked to the outer circumferential surface of the flange, and is formed on the inside of the cover side so that the flange and the first packing ring are seated. Decanting while forming a ring seating groove, a coupling step that is inserted into the coupling groove to form an inner circumferential surface such as the inner circumferential surface of the centrifugal separation body, and the centrifuged sample forming a guide surface such as the inner circumferential surface of the centrifugation body It characterized in that it consists of a discharge portion protruding upwardly so that it can be decanted toward the container.

In the present invention, the decanting container includes a decanting base body in which a decanting chamber is formed inside so that the components discharged from the centrifugal separator are separated and stored, and the upper opening of the decanting base body is sealed, but component extraction from the decanting base body is Consisting of a decanting container cover having a discharge port and a ventilation port formed so as to be possible, to one side of the decanting base body, such as the discharge part so that low-density components discharged from the discharge part of the centrifugal separation base body flow. An inlet portion having a guide surface is formed, and the discharge portion includes an upper flange of a predetermined shape formed by extending upward at a predetermined distance from the cover body, a cover coupling stepped extending from the upper flange to an upper side and forming a stepped inward, Consisting of an outlet of a predetermined shape formed inside the cover coupling step, the inlet portion is seated on the lid coupling step of the discharge portion and has an inlet having the same guide surface as the outlet, and an outer side of the lid coupling step and a second packing ring It is composed of a coupling hook of a predetermined shape that is hooked to the upper flange while surrounding the cover, and is interposed between the outer side of the cover coupling step and the coupling hook, and comprising a second packing ring for sealing the discharge portion and the inlet portion. do.

In the decanting kit for easy separation and extraction of each component according to the present invention, components of a sample are concentrated and separated for each component by a centrifugal separator, and a specific component can be separated and stored using a component recovery container. And, the selective extraction process of specific ingredients brings about an effect that makes it easier.

In addition, the present invention has the effect of completely extracting only specific components because it is possible to prevent unnecessary components from being included in the extraction components by limiting the moving distance of the plotter moving up and down in the centrifugal container.

In addition, the present invention can separate only desired components from blood or bone marrow used for autologous bone marrow therapy and autologous blood therapy, and can achieve medical results such as enhancing the therapeutic effect using the separated components (platelet-rich plasma, etc.). It is, of course, a very useful invention that can greatly contribute to the development of the medical industry in connection therewith.

1 is a perspective view of a decanting kit for easy separation and extraction of each component according to the present invention.
Figure 2 is an exploded perspective view of the centrifuge container of the decanting kit for easy separation and extraction of each component according to the present invention.
Figure 3 is a bottom perspective view of the centrifugal separator cover according to the present invention.
Figure 4 is an exploded perspective view of the decanting container of the decanting kit for easy separation and extraction of each component according to the present invention.
Figure 5 is a perspective view as seen from the bottom side of the decanting container according to the present invention.
Figure 6 is a cross-sectional view of a decanting kit for easy separation and extraction of each component according to the present invention.
7 is an operation diagram of a decanting kit for easy separation and extraction of each component according to the present invention.
8 and 9 are exemplary views illustrating the operation of the centrifuge rotor of the decanting kit for easy separation and extraction of each component according to the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those of ordinary skill in the art to which the present invention pertains can easily implement the present invention. First of all, in adding reference numerals to elements of the drawings, it should be noted that the same elements are to have the same numerals as possible even if they are indicated on different drawings. In addition, in describing the present invention, if it is determined that a detailed description of a related known configuration or function may obscure the subject matter of the present invention, a detailed description thereof will be omitted.

1 is a perspective view of a decanting kit for easy separation and extraction by components according to the present invention, FIG. 2 is an exploded perspective view of a centrifuge container of a decanting kit for easy separation and extraction by components according to the present invention, and FIG. 3 is the present invention Is an exploded perspective view of the decanting container of the decanting kit for easy separation and extraction by components according to the present invention, and FIG. 5 is a perspective view as viewed from the bottom side of the decanting container according to the present invention 6 is a cross-sectional view of a decanting kit for easy separation and extraction for each component according to the present invention, FIG. 7 is an operation diagram of a decanting kit for easy separation and extraction for each component according to the present invention, and FIGS. 8 and 9 It is an exemplary diagram illustrating the operation of the centrifuge rotor of the decanting kit for easy separation and extraction of each component according to the present invention.

As shown in FIG. 1, the decanting kit 1 for easy separation and extraction of each component according to the present invention is a centrifugal separator in which a sample contained inside is concentrated and separated for each component due to a difference in density during centrifugation using a centrifuge. (10) And, a decanting container (20) in which components discharged from the centrifugal separator (10), which are connectedly mounted on one upper side of the centrifugal separator (10) and in a state of being inclined close to horizontal by the centrifugal force during centrifugation, are separated and stored Consists of including.

The centrifuge 3 will be described with reference to FIG. 8. Centrifuge (3) is a device used for the purpose of separating and recovering only specific components from complex fluids (hereinafter referred to as "samples"), such as separating blood cells and plasma from blood or stem cells from bone marrow. 8 and 9, a rotor 2 that rotates horizontally when the device is driven is installed inside, and a plurality of rotating rotors rotate together when the rotor 2 is rotated in a state in which the decanting kit 1 is accommodated and mounted. A bucket (2-1) is provided.

In addition, at one side of the circumference of the bucket 2-1, as shown in FIG. 9, a hole 2-2 is formed to look inside the decanting kit 1 or to reduce the load at the rear.

The decanting kit (1) serves as a storage medium for concentration and separation of each component of a sample, and can contain a certain amount of a sample to be subjected to centrifugation, and during centrifugation, a centrifugation chamber 14 in which the sample is concentrated and separated for each component. ) Is provided, the centrifugal separation container cover 12, which seals the upper opening of the centrifugal separation base 11 and allows sample injection into the centrifugation chamber 14, and the centrifugal separation base body ( 11) A plotter 13 that moves along the longitudinal direction of the centrifugal main body 11 so as to be located at the boundary of each component based on the density difference of each component of the sample to be centrifuged inside, and the top and bottom of the plotter 13 It consists of an insert cover 15 that is seated in the inner centrifugation chamber 14 of the centrifugal separation body 11 in order to limit the movement.

As shown in Fig. 2, the centrifugal separator 10 is formed outward from the upper end of the centrifugal separator 11 and the centrifugal separator 11 so that the centrifugal separator cover 12 is hooked. The flange 114 and the ring step 1113 on which the first packing ring 30a for sealing the centrifugal separator cover 12 and the centrifugal separator 11 is seated and formed on the inside of the flange 114 , It is formed on the inner side of the ring step 113 and consists of a coupling groove 112 for forming the same inner circumferential surface as the inner side of the centrifugation container cover 12, and the upper end 111 of the centrifugal separation body 11 is a ring step It constitutes the coupling groove 112 together with (113). In addition, a fastening fixing groove 116 for fixing the coupling position of the centrifugal separator cover 12 is formed on one side of the flange 114. The fastening fixing groove 116 may fix the mounting position of the cover 12 while the fastening fixing protrusion 121-2 formed on the centrifugal separator cover 12 is inserted.

As shown in Figures 2 and 3, the centrifugal separator cover 12 includes a centrifugal separator cover body 121 having a cover side surface 121-1 that is hooked to the outer circumferential surface of the flange 114, and a cover It is formed on the inner side of the side (121-1) and is inserted into the ring seating groove 129 in which the flange 114 and the first packing ring (30a) are seated, and the same as the inner circumferential surface of the main body for centrifugation. Discharge protruding upward so that the centrifuged component can be decanted toward the decanting container 20 by forming a coupling step 127 for forming an inner circumferential surface and an inner surface such as the inner circumferential surface of the centrifugal separator 11 and a certain section It consists of a part (125). The discharge unit 125 includes a guide plate 125-1 forming an inner circumferential surface of the centrifugal separation body 11 and an inner surface such as a predetermined section, and a discharge port 125-2 of a predetermined shape formed by the guide plate 125-1. ), and an upper flange 125-3 formed by extending upward from the centrifugal container cover body 121 by a predetermined distance, and a cover extending a predetermined distance upward from the upper flange 125-3 to form a stepped inward. It consists of a coupling step (125-4). The guide plate 125-1 is formed in the same inner surface as the inner circumferential surface of the centrifugal body 11 and a certain section, thereby forming a flow path so that the centrifuged specific sample can easily move to the decanting container 20. The centrifugation container cover 12 is provided with an injection port 126 and a ventilation port 122, and the sample is injected into the chamber 14 of the centrifugal container through the injection port 126, and through the injection port 126. When the sample is injected, air can be discharged to the outside through the ventilation port 122.

4 and 5 show the decanting container 20. The decanting container 20 seals the upper opening of the decanting base body 21 and the decanting chamber 23 formed inside the decanting chamber 23 so that the components discharged from the centrifugal separator 10 are separated and stored. However, it is composed of a decanting container cover 22 having a discharge port 221 and a ventilation port 222 to enable extraction of components from the decanting base body 21. The decanting base body 21 is provided with a decanting chamber 213 and an inlet 211. The inlet portion 211 includes an outer guide plate 211-1 that is hooked to the upper flange 125-3, an inner guide plate 211-2 formed in the same manner as the guide plate 125-1, and an outer guide plate ( 211-1) and an inlet coupling groove 211-4 formed by the inner guide plate 211-2, and an inlet opening 211-3 formed by the inner guide plate 211-2. The inner guide plate 211-2 is seated on the cover coupling stepped step 125-4, and a second packing ring 30b is interposed between the outer surface of the lid coupling stepped step 125-4 and the outer guide plate 211-1 do. A portion that passes through the inlet 211-3 and enters the decanting chamber 213 is formed with a barrier 214 so that the centrifuged component can smoothly move to the decanting chamber 213. The decanting chamber 213 is formed by an inclined surface 218 formed downward at a predetermined angle to both sides and a storage groove 219 in which the centrifuged component passing through the inclined surface 218 is stored. An extension end 216 extending upward of a predetermined length and a decanting flange 217 formed on an outer surface of the extension end are provided at an upper end of the decanting container 21.

The decanting container cover 22 is formed on the decanting container cover body 221, the outer surface portion 223 extending downward from the decanting container cover body 221 to form an edge, and the decanting container cover body 221 It consists of an extraction port 222 and a ventilation port 224. The outer surface portion 223 of the decanting container cover body is hooked to the decanting flange 217, and between the outer surface portion 223 and the extension end 216 coupled to the decanting flange 217, a third packing ring for sealing ( 30c) is interposed.

The plotter 13 is intended to prevent the sedimentation component and the floating component from being mixed with each other through the boundary between each component of the sample concentrated and separated for each component inside the centrifugal separator 11, as shown in FIG. , The plotter 13 may be configured in the form of a basket or plate. A plurality of scaffolds (132) are provided at the bottom of the centrifuge chamber (14) to facilitate precipitation of dense components under the centrifugation chamber (14) by forming a sufficient gap with the bottom plate of the plotter (11). It is protruding from the bottom of the plate, and through the center of the bottom plate of the plotter, a through hole 131 is formed so that the high-density component can pass through and settle to the bottom of the bottom plate during centrifugation.

In addition, in the center of the upper surface of the bottom plate of the through hole 131, a blunt portion 133 is protruded to block the reverse flow of the precipitated component while the component is easily precipitated through the through hole 131, and at the upper end of the through hole 131 An expansion part that facilitates the discharge of components with high density may be further formed.

A detailed description of the operation of the decanting kit 1 for easy separation and extraction of each component according to the present invention having the above configuration is as follows.

First, the sample contained in the syringe is transferred to the centrifuge container 10 for the separation of components such as blood or bone marrow collected from the patient using a syringe.

In more detail, it is injected into the centrifugation chamber 14 in the main body 11 for centrifugation through the injection port portion 126 formed in the cover 12 of the centrifugal separator 10.

When the sample is injected into the centrifugal separator 11, the air remaining in the centrifugation chamber 14 is discharged to the outside through the ventilation port 122 formed in the centrifugation container cover 12. The internal pressure of (11) is kept constant.

Next, as shown in Figs. 8 to 9, the decanting kit 1 of the present invention is accommodated and mounted in each bucket 2-1 hinged to the rotor 2 of the centrifuge 3 and then centrifuged. By driving the separator 3 to rotate the rotor 2 horizontally, the components of the sample contained in the centrifugation chamber 14 of the main body for centrifugation 11 are in the order of density from low to high by centrifugal force. Separated.

For example, in the case of blood, blood cells, which are components with high density, are concentrated and separated in the lower part of the plotter 13 in the centrifugation chamber 14 of the centrifugal separation body 11 based on the plotter 13, and the plotter 13 In the upper part of the plasma, the plasma with low density is concentrated and separated. The plotter 13 moves along the length direction by receiving buoyancy by components having different densities.

During the separation of the sample by component through this process, the plotter 13 moves (floats) along the longitudinal direction of the centrifugal separator 11 due to the difference in density of each component, and is located at the boundary between the high density component and the low component. It is done. However, the movement of the plotter 13 along the longitudinal direction is limited by the insert cover 15, and the presence of the insert cover 15 allows the plotter 13 to separate only a certain amount of components, so that it is separated. Can increase the efficiency of

As the rotor 2 rotates according to the operation of the centrifuge 3 as described above, the sample in the centrifuge container 11 is centrifuged, and when the operation of the centrifuge stops, a separate angle maintaining means (not shown) As a result, the centrifugal separator 11 is operated to be inclined at a certain angle with the bucket 2-1, and accordingly, the components on the upper side of the plotter 13 of the centrifugal chamber 14 are reduced to the decanting container 20 ). When all the components above the plotter 13 flow into the decanting container 20, the angle maintaining means returns the bucket 2-1 to its original state, and decanting is completed.

Next, the separated component is extracted from the decanting container 20 of the decanting kit 1, and in order to extract the separated component, it is extracted with a syringe through the extraction port 222.

The above description is merely illustrative of the technical idea of the present invention, and those of ordinary skill in the art to which the present invention pertains within the scope not departing from the essential characteristics of the present invention, as shown in FIG. However, various modifications, changes and substitutions will be possible. Therefore, the embodiments disclosed in the present invention and the accompanying drawings are not intended to limit the technical idea of the present invention, but are intended to describe, and the scope of the technical idea of the present invention is not limited by these embodiments and the accompanying drawings. . The scope of protection of the present invention should be interpreted by the following claims, and all technical ideas within the scope equivalent thereto should be construed as being included in the scope of the present invention.

1: decanting kit 2: rotor
3: centrifuge
10: centrifugation container 11: base body for centrifugation
12: centrifugal separator cover 13: plotter
14: centrifugal separation chamber 15: insert cover
20: decanting container 21: basic body for decanting
22: decanting container cover
30(30a, 30b, 30c): packing ring

Claims (3)

During centrifugation using a centrifugal separator, a centrifuge container is formed so that the samples contained therein are concentrated and separated for each component by the difference in density;
A decanting container which is connectedly mounted on the upper side of the centrifugal separator and in which the components separated from the centrifugal separator are decanted while inclined at a certain inclination;
A plotter provided inside the centrifuge container, moving along the longitudinal direction of the centrifuge container, and positioned at the boundary of the centrifuged component due to the difference in density of each component of the centrifuged sample; And
An insert cover for limiting a moving distance of a plotter seated inside the centrifugal separator and moving along the longitudinal direction of the centrifugal separator;
Decanting kit for easy separation and extraction of each component comprising a.
The method of claim 1,
The centrifugal separation vessel includes a centrifugation base body having a centrifugation chamber formed inside so that a certain amount of a sample to be centrifuged is stored and the sample is concentrated and separated for each component during centrifugation, and the upper opening of the centrifugation base body is sealed. However, a centrifugal separation container cover provided with an injection port for injecting a sample into the centrifugal separator, a vent port through which air is discharged when the sample is injected, and a discharge part through which the centrifuged components are discharged, and the centrifugal separation body and the centrifuge It is interposed between the separation container cover and consists of a first packing ring to seal the inside,
The centrifugal separation body includes a flange formed from an upper end of the main body to the outside to which the centrifugation container cover is hooked, and a first packing ring for sealing the centrifugal separation container cover and the body is seated on the inside of the flange. And a coupling groove formed on the inner side of the ring stepped to form the same inner circumferential surface as the inner side of the centrifugal separator cover,
The centrifugal separation container cover, a centrifugal separation container cover body having a cover side that is hooked to the outer circumferential surface of the flange, and a ring seating groove formed on the inside of the cover side and in which the flange and the first packing ring are seated. , A coupling step for being inserted into the coupling groove to form an inner circumferential surface such as the inner circumferential surface of the centrifugal separation body, and the centrifuged sample to be decanted toward the decanting container while forming a guide surface such as the inner circumferential surface of the centrifugal separation body and a predetermined section. A decanting kit for easy separation and extraction of each component, characterized in that it consists of a discharge portion protruding upwardly so that it can be extracted.
The method of claim 2,
The decanting container includes a decanting base body in which a decanting chamber is formed inside so that the components discharged from the centrifugal separator are separated and stored, and the upper opening of the decanting base body is sealed, but components can be extracted from the decanting base body. It is composed of a decanting container cover formed with a discharge port and a ventilation port,
On one side of the decanting body, an inlet portion having a guide surface such as the discharge portion is formed so that the low-density component discharged from the discharge portion of the centrifugal separation body flows,
The discharge unit may include an upper flange of a predetermined shape formed by extending a predetermined distance upward from the cover body, a cover coupling stepped extending upward from the upper flange to form a stepped inward, and formed inside the lid coupling stepped. It is composed of an outlet of a certain shape,
The inlet part is composed of an inlet having the same guide surface as the outlet by being seated on the cover coupling step of the discharge part, and a fixed-shaped coupling hook that is hooked to the upper flange while enclosing the outer side of the lid coupling step and the second packing ring. under,
A decanting kit for easy separation and extraction of each component, comprising a second packing ring interposed between the outer side of the cover coupling step and the coupling hook to seal the discharge portion and the inlet portion.

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