KR101874548B1 - Decanting-kit container for divide extraction easy by ingredient specific - Google Patents

Decanting-kit container for divide extraction easy by ingredient specific Download PDF

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Publication number
KR101874548B1
KR101874548B1 KR1020170054804A KR20170054804A KR101874548B1 KR 101874548 B1 KR101874548 B1 KR 101874548B1 KR 1020170054804 A KR1020170054804 A KR 1020170054804A KR 20170054804 A KR20170054804 A KR 20170054804A KR 101874548 B1 KR101874548 B1 KR 101874548B1
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KR
South Korea
Prior art keywords
container
chamber
components
sample
component
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KR1020170054804A
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Korean (ko)
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신현순
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신현순
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces

Abstract

The present invention relates to a decanting kit facilitating separation and extraction per components. More specifically, the decanting kit facilitates selective extraction of components concentrated and separated according to density differences in the separation of blood or bone marrow components using a centrifugal separator. According to the present invention, the decanting kit comprises: a centrifugal separation container which enables a sample contained in the centrifugal separation container to be concentrated and separated by the components according to the density differences when performing a centrifugal separation process using a centrifugal separator; and a component recovery container which is linked to and mounted on one side of the centrifugal separation container, and which separates and stores the components flown out from the centrifugal separation container that has become an almost horizontally inclined state by centrifugal force during centrifugal separation.

Description

TECHNICAL FIELD [0001] The present invention relates to a decanting kit,

The present invention relates to a decanting kit which is easy to separate and extract by component, and more particularly to a decanting kit for separating components of blood or bone marrow using a centrifuge, The present invention relates to a decanting kit which is easy to separate and extract by components.

If suspended suspension with floating material is kept still, the denser material slowly sinks to the bottom due to the effect of gravity, and the denser material slowly moves to the upper layer. This process is called sedimentation (sediment).

Thus, when the materials with different densities are mixed, precipitation occurs, and over time, the mixture can be separated according to the density difference. In other words, the density difference between the mixtures increases as the gravity, which is the force for separating the mixture, becomes larger. Therefore, if the gravity is artificially increased, the precipitation phenomenon can be accelerated.

Therefore, it is easy to accelerate the precipitation phenomenon by using centrifugal force instead of gravity. This process is called centrifugation. A centrifugal separator (centrifugal separator) is a device used to separate, purify, and concentrate a substance having a different component or specific gravity (density difference) by using the principle of centrifugal separation described above. , Wastewater treatment, uranium enrichment, production, and laboratory use.

Among them, the medical centrifuge has a purpose of separating constituents for analysis of blood, urine, saliva, etc., but also for platelet-rich plasma extraction. Herein, the platelet-rich plasma (PRP) can be obtained by centrifugation of a complex fluid (hereinafter referred to as "sample") such as bone marrow or blood, and is highly enrichment , High concentration) Plasma components, which contain several growth factors, heal wound healing and reconstructs the damaged area. For example, it is used for reconstructing a damaged part by injecting a damaged part such as a ligament, a cartilage, etc., since it uses its own bone marrow or blood, so there is no side effect and treatment effect is fast.

On the other hand, the centrifugal separator described above is divided into various types according to the quantity of the sample to be centrifuged, the rotational speed, the type of the rotor, and the centrifugal force of the rotor rotational motion, And a centrifugal separator for performing centrifugal separation.

Based on this technical idea, Korean Patent Publication No. 10-2007-0026316 discloses a disposable container for centrifugation and treatment of a fluid biological material, The centrifugal component is sucked into a third canola (hose) which is connected to the bottom of the container by inserting a needle into the third canola (hose) chamber, the centrifuged components are mixed again due to the change in the sample content in the sample (in the sample fresh water drop) during the suction extraction (suction) using the syringe, So it is difficult to extract only specific ingredients.

In addition, since an adhesive is used for coupling between the lid and the container, it is difficult to assemble and manufacture the centrifuge container. In addition, since the sample injection port and the extraction port are formed of a rubber material, There is a problem that it is not easy to inject the sample or extract the separated components separately.

Korean Patent Publication No. 10-2007-0026316 (published date: 2007. 03. 08.)

The object of the present invention is to solve the above problems, and it is an object of the present invention to provide a centrifugal separator for separating a sample by centrifugal force and the specific gravity of each component, Which is easy to separate and extract, so that the extraction of the characteristic component from the separated components can be performed perfectly, easily, and promptly.

It is another object of the present invention to provide a centrifugal separator for concentrating and separating components of a sample and a component recovery container in which specific components with low density are separated and stored, The present invention provides a decanting kit which is easy to separate and extract by components, which can greatly improve the operation efficiency of a specific component extraction as well as the centrifugal separation of the sample as well as the storage and separation of the enriched and separated components.

In order to accomplish the above object, the present invention provides a centrifugal separator, comprising: a centrifugal separator for concentrating and separating components contained therein by density difference during centrifugal separation using a centrifugal separator; And a component collecting container connected to one side of the centrifuge vessel and separated from the centrifugal separator and being separated from the centrifugal separator vessel by a centrifugal force during centrifugal separation, .

The centrifuge vessel may further include a first container body having a first chamber that can contain a predetermined amount of a sample to be subjected to centrifugal separation and in which a sample is concentrated and separated by centrifugation, A first container lid which is hermetically closed and capable of injecting a sample into the first chamber, and a second container lid which is provided in the first container body and is located at a boundary between the respective components based on the density difference of components of the centrifuged sample. And a floater that moves along the longitudinal direction of the first container body.

In addition, the ingredient recovery container includes a second container body having a second chamber in which the components flowing out from the centrifuge container are separated and moved, and an upper end of the opening of the second container body is closed, And an outlet pipe portion protruding from the first container lid to allow a component having a lower density to flow from the first container lid to be connected to one side of the second container lid, A flow channel for guiding the flow of the component flowing into the second chamber to the second chamber and guiding the flow of the component flowing through the flow channel is connected and a portion of the flow- A gentle inclined surface is formed so that the cell of the cell is not destroyed.

In addition, the floater may have a sufficient gap with the bottom surface of the first container body, so that when the centrifugal separator is used, a plurality of scaffolds, which are free from sedimentation of components having a high density toward the lower side of the first chamber, Wherein one or more through holes are formed through a predetermined portion of the bottom plate so as to pass through a component having a high density during centrifugation and to settle down to the first chamber, In the central portion of the upper surface, a dull portion for preventing the backflow of the precipitating component is easily formed while the component is easily precipitated through the through hole, and an expanded portion is formed on the inner surface of the upper end of the through hole for facilitating the discharge of the dense component .

In addition, the first container lid is connected to the ingredient recovery container, and has an outlet for allowing a low-density component to flow out from the first container body that is in a state of being inclined horizontally due to the centrifugal force during centrifugal separation And an injection port portion for injecting a sample into the first chamber are each protruded from a predetermined portion and the second container lid is provided with an extraction port portion for extracting a component contained in the second chamber, The port portion and the vent port portion for controlling the air flow in combination with the sample injection and extraction using the extraction port portion out of the first and second chambers are respectively protruded from the predetermined portion.

In addition, the floater may have a shape of a basket formed such that an outer surface thereof is connected to an upper side of a rim of the bottom plate, the outer side surface of the first container is larger in diameter and tilted toward an upper side when viewed in section, A protrusion is formed to protrude from the outer surface of the upper end of the outer surface so as to improve the buoyancy by reducing the movement friction through minimizing the contact area with the inner surface.

In addition, the injection port portion and the extraction port portion include a packing holder for preventing any leakage of the sample contained in the first chamber and the second chamber, and the packing holder is made of a flexible material such as rubber or silicone Wherein the insertion hole through which the needle of the needle passes is formed along the longitudinal direction at the center and the needle of the needle can penetrate the upper side of the insertion hole while preventing any leakage of the sample contained in the first chamber and the second chamber Is formed.

As can be clearly understood from the above description, the decanting kit of the present invention, which is easy to separate and extract by components, can concentrate and separate the components of the sample by the centrifuge container, and can separate and store specific components using the component recovery container Accordingly, the separation process of the component of the sample and the selective extraction process of the specific component are more effective.

In addition, a specific component is extracted by a floater floating in a state where it is placed at the boundary of the separation component according to the difference in density of the components separated by components in the inner space (first chamber) of the first container body by centrifugation The other components are not mixed with the extracted components so that the extraction of the specific components is more completely performed.

In addition, according to achievement of various effects as described above, the separation of components from the blood or bone marrow used for autologous bone marrow treatment and autologous blood treatment becomes perfect, and the therapeutic effect using the separated component (platelet rich plasma etc.) And it is a very useful invention that can greatly contribute to the development of medical industry related to this.

1 (a) and 1 (b) are perspective views showing an outer structure of a decantering kit which can be easily separated and extracted according to the present invention.
FIG. 2 is an exploded perspective view showing a constitutional relationship of a decanting kit which can be easily separated and extracted according to the present invention. FIG.
FIG. 3A is a cross-sectional view illustrating an internal structure of a decanting kit which can be easily separated and extracted according to the present invention. FIG.
FIG. 3B is an exemplary sectional view showing a process of separating components by a decanting kit which is easy to separate and extract according to the present invention. FIG.
FIG. 4A and FIG. 4B are cross-sectional views illustrating various structures of a plotter in a decanting kit in which separation and extraction of components are easy according to the present invention. FIG.
FIG. 5 and FIG. 6 are usage states showing examples of using a decanting kit which can be easily separated and extracted according to the present invention.
FIG. 7A and FIG. 7B are views showing operation examples of a centrifugal rotor showing an embodiment of a decanting kit in which separation and extraction of components according to the present invention is easy; FIG.
FIG. 8 is a sectional view showing another structure of a decanting kit which is easy to separate and extract by components according to the present invention. FIG.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention. First, in adding reference numerals to the constituents of the drawings, it is to be noted that the same constituents are denoted by the same reference numerals as much as possible even if they are displayed on different drawings. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

1 (a) and 1 (b) are perspective views showing an outer structure of a decanting kit which can be easily separated and extracted according to the present invention, and FIG. 2 is a perspective view of a decanting kit FIG. 3A is a cross-sectional view showing an internal structure of a decanting kit which can be easily separated and extracted according to the present invention, and FIG. 3B is a cross-sectional view showing a decanting kit according to the present invention, FIGS. 4A and 4B are cross-sectional views showing various structures of a plotter in a decanting kit which can be easily separated and extracted according to the present invention, and FIGS. 5 and 6 are cross- FIG. 7A and FIG. 7B are explanatory diagrams showing a use state of the decanting kit according to the present invention, An operation example of the separator rotor, and Fig. 8 is a sectional view showing another structure of the decanting kit easy Ingredients extracted and separated according to the present invention.

As shown in FIGS. 1 and 2, the decanting kit (A), which is easy to separate and extract by components according to the present invention, is characterized in that the sample contained in the centrifugation using a centrifuge is concentrated and separated A centrifugal separator (1) comprising: a centrifugal separator (1); a component recovery device (1) for separating and storing components flowing out from a centrifugal separator (1) attached to one side of the centrifugal separator And a container (2).

Here, the centrifugal separator 900 is used for separating and recovering only specific components from a complex fluid (hereinafter referred to as a "sample") such as separation of blood cells from plasma or separation of stem cells from bone marrow 5 and 6, a rotor 910 which rotates horizontally when the device is driven is installed therein, and the rotor 910 is provided with a receiving portion for receiving and mounting the centrifuge container 1, There is provided a plurality of buckets 911 for performing a rotating movement in rotation of the rotor 910 to the state of the rotor 910.

As shown in FIG. 6, a through hole 911-1 through which the inside of the centrifuge container 1 can be seen is preferably penetrated through one side of the periphery of the bucket 911.

The centrifugal separation vessel 1 serves as a storage medium for concentration and separation of the components of the sample. The centrifugal separation vessel 1 can contain a predetermined amount of a sample to be subjected to centrifugal separation, and the first chamber A first container lid 12 which hermetically closes an opening top of the first container main body 11 and into which the sample can be injected into the first chamber 11a, And a floater 13 which is provided in the container body 11 and moves along the longitudinal direction of the first container body 11 so as to be located at the boundary of the respective components based on the density difference of the components of the centrifuged sample.

As shown in FIGS. 3A and 3B, the first container lid 12 forms a disk-like structure capable of covering and sealing the upper end of the opening of the first container body 11, An outlet pipe portion 121 connected to the component recovery container 2 and capable of flowing a component having a lower density from the first container body 11 that has become inclined horizontally due to centrifugal force during centrifugal separation, And an injection port 122 for injecting a sample into the first chamber 11a is formed to protrude from another part of the upper end.

A packing holder 122-1 for preventing any leakage of the sample contained in the first chamber 11a is contained in the injection port portion 23. The packing holder 122-1 has rubber A flexible material such as silicon or the like is formed in the center along the longitudinal direction and an insertion hole 122-11 through which the needle of the needle is passed is formed at the center of the insertion hole 122-11, A blocking layer 122-12 for preventing any leakage of the sample contained in the first chamber 11a is formed.

The floater 13 is provided to prevent the sedimented component and the suspended component from being mixed with each other through the boundaries of the respective components of the sample concentrated and separated in the first container body 11, A plurality of scaffolding plates 131 are formed protruding from the bottom surface of the bottom plate so as to allow precipitation of a component having a high density below the first chamber 11a during centrifugation, One or more through holes 132 are formed through the predetermined portion of the bottom plate so as to pass through a component having a high density during centrifugation and to settle down to the first chamber 11a.

In the center of the upper surface of the bottom plate on the upper side of the through hole 132, a barrier 133 for easily precipitating components through the through hole 132 but blocking the backflow of the precipitating component is protruded, (132-1) for smoothly discharging a component having a high density is further formed on the inner surface of the upper portion of the upper portion (132).

4A and 4B, the plotter 13 may have various shapes such as a basket or a plate. In the case of the first embodiment in the form of a basket, as shown in FIGS. 4A and 4B, As shown in the drawing, the outer surface of the bottom plate is formed so that the outer surface thereof increases in diameter toward the upper side and tilts toward the upper side when viewed in section, and minimizes the contact area with the inner surface of the first container body 11 The protrusion 134 may be formed to protrude from the outer surface of the upper end of the outer surface.

On the other hand, in the case of the second embodiment in the form of a plate, as shown in FIG. 4B, the outer surface and the protrusion 134 described above are omitted, A plurality of scaffolds 131 'are formed protruding from the bottom surface of the bottom of the first chamber 11a so as to allow precipitation of components having a high density at the time of centrifugal separation, One or more through holes 132 'are formed through the through holes 132' so as to be deposited to the lower side of the first chamber 11a through the through holes 132 '. At the center of the upper surface of the through holes 132' (132 ') is formed on the inner surface of the upper portion of the through-hole (13' 2) so as to facilitate the discharge of a component having a higher density. The depressed portion (133 ' 1 ').

The component collection container 2 is configured such that when the centrifuge container 1 is tilted horizontally due to centrifugal force due to centrifugal separation, components in which the density of the sample contained in the first chamber 11a is low A second container body 21 provided with a second chamber 21a in which the components flowing out from the centrifuge container 1 are separated and moved and stored as a recovery medium for moving storage, And a second container lid 22 which is capable of extracting the component from the second chamber 21a while sealing the upper end of the opening of the second lid 21a.

The outlet tube portion 121 is connected to one side of the second container body 21 in a state of being assembled to the outlet tube portion 121 protruding from the first container lid 12 of the centrifuge container 1, The inlet chamber 211 is connected to the second chamber 21a and the inflow hole 211 for guiding the inflow of the components flowing through the second chamber body 21 and the second chamber body 21 as shown in FIG. A portion of the slope 211-1 forms a gentle slope 211-1 so that the cell of the inflow component due to the drop impact is not destroyed.

The second container lid 22 has a disk-like structure that can cover and close the upper end of the opening of the second container body 21 while extracting the components contained in the second chamber 21a from above the upper part. And the first and second chambers 11a and 21a using the injection port part 122 and the extraction port part 221 are formed to protrude from the upper part of the upper part, A vent port portion 222 for venting air in and out is connected to the biological substance (hereinafter referred to as "sample") in conjunction with the transfer (injection and extraction) of the sample.

Like the injection port part 122, the extraction port part 221 includes a packing holder 221-1 for preventing any component contained in the second chamber 21a from leaking out. The packing holder 221-1 is also made of a flexible material such as rubber or silicon. The insertion hole 221-11 through which the needle passes is formed at the center along the longitudinal direction, And a blocking film 221-12 is formed on the side of the second chamber 21a to prevent any leakage of the components contained in the second chamber 21a.

(P) shows a rubber packing (not shown) for sealing the gap between the first container body 11 and the first container lid 12 and between the second container body 21 and the second container lid 22 rubber packing.

Hereinafter, the operation of the decanting kit (A), which is easy to separate and extract for each component according to the present invention, will be described in detail.

First, the sample contained in the syringe is transferred to the centrifuge container 1 for separation of components of the sample such as blood or bone marrow collected from the patient by using a syringe.

More specifically, the centrifugal separator 1 is connected to the first chamber 11a of the centrifugal separation vessel 1 through the injection port portion 122 formed in the first vessel lid 12 of the centrifugal separation vessel 1, So that the tip of the needle can penetrate the blocking film of the first packing holder 122-1 and reach the first chamber 11a through the insertion hole 122-11 .

When the sample is injected into the first container body 11 according to the above procedure, the air staying in the first chamber 11a flows into the second container lid 22 of the ingredient collecting container 2, So that the internal pressure of the first container body 11 is balancedly maintained as it is discharged to the outside through the port portion 222.

6 to 7A, the decanting kit A of the present invention is accommodated in each of the buckets 911 hinged to the rotor 910 of the centrifugal separator 900, The centrifugal separation process is performed in which the components of the sample contained in the first chamber 11a of the first container body 11 are separated through driving the rotor 900 to rotate the rotor 910 horizontally, This is possible as the sample constituents have different densities.

7B, the centrifuge vessel 1 of the decanting kit A of the present invention, which is accommodated in the bucket 911 by the influence of the centrifugal force generated in the horizontal rotation of the rotor 910, So that the denser component of the sample contained in the first chamber 11a of the first container body 11 is precipitated in the lower layer portion , And a component with a low density floats to the upper layer.

For example, in the case of blood, a blood component, which is a high density component, is concentrated and separated in the lower portion of the first chamber 11a of the first container body 11, and a plasma having a low density is concentrated and separated in the upper portion.

During this process, the floater 13 moves (floats) in the first chamber 11a along the longitudinal direction of the first container body 11 due to the difference in density of the components, And the lower portion of the first chamber 11a is discharged through the through hole 132 formed through the bottom plate of the floater 13, This is possible due to the influence of buoyancy generated in

At this time, since the outer surface of the bottom plate of the floater 13 is not completely adhered to the bottom surface of the first container body 11 by the foot plate 131 of the floater 13, 132, and the bottom of the first container body 11 can be settled.

In addition, by forming the expanded portion 132-1 of the floater 13, the dense component can be smoothly discharged through the through hole 132. When the floater 13 is formed as a basket, The contact area between the first container body 11 and the first container body 11 can be smoothly reduced due to the minimized contact friction between the first container body 11 and the first container body 11.

The rotor 910 rotates according to the operation of the centrifugal separator 900 as described above. At this time, the center of gravity of the sample contained in the centrifugal separator 1 moves to the outside due to the centrifugal force, The centrifugal separator 1 is tilted to one side with the bucket 911 by the means (not shown). Even if the centrifugal separation process is stopped (rotation of the rotor is stopped) The tilting angle of the separation vessel 1 is maintained.

Accordingly, a component having a low density among the components concentrated and separated in components in the first chamber 11a flows into the component collection container 2 through the outlet pipe portion 121, 121 to the second chamber 21a of the second container body 21 of the component recovery container 2 via the inflow hole 211 connected thereto.

Here, the components passing through the inlet stem 211 flow smoothly into the second chamber 21a as guided by the slope 211-1, so that the cell of the component for separation and extraction is broken It is possible to prevent that

If a specific component having a low density among the constituent components of the sample is separated and transferred to the second chamber 21a as described above, the specific component can be extracted, After the needle of the syringe for extracting the components is inserted into the port portion 221, the tip of the needle penetrates the blocking membrane 221-12 of the packing holder 221-1 in the extraction port portion 221, It is possible to insert it sufficiently to reach the second chamber 21a through the insertion holes 221-111 of the holder 221-1.

According to the above-described process, a specific component of the sample separated by components in the second chamber 21a of the second container body 21 can be extracted. In this case, The external pressure is introduced into the first and second container bodies 11 and 21, thereby maintaining the internal pressure balanced.

Subsequently, when the amount of sample water contained in the first chamber 11a is changed in the process of component extraction as described above, components having different densities can be mixed based on the operation, Is prevented by the plotter (13).

Finally, when the exaggeration of the separation and transfer of a specific component into the second chamber 21a of the second container body 21 is completed, the specific component separated and transferred to the second chamber 21a is immediately So that the component recovery container 2 can be separated from the centrifuge container 1 and separated and stored in the state.

According to the decanting kit (A), which is easy to separate and extract according to the present invention, centrifugal separation of components such as blood or bone marrow is easier than that of the centrifugal separator (1) A specimen injected into and centrifuged from the first chamber 11a of the main body 11 is transferred from the first container body 11 to the second chamber 21a of the second container body 21 It is possible to obtain an effect that it is easier to selectively extract the components.

In addition, in the process of extracting a specific component from the second chamber 21a, the components that have already been separated due to changes in the sample storage amount in the first chamber 11a are mixed, There is an effect that mixing of the respective components is prevented by the floater 13 in which the bottom plate is positioned at the boundary of the respective separation components, thereby being sufficiently possible.

[0060] The present invention is not limited to the above-described embodiments, and various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims. , Various modifications, alterations, and substitutions will be possible. Therefore, the embodiments disclosed in the present invention and the accompanying drawings are intended to illustrate and not to limit the technical spirit of the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments and the accompanying drawings . The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of the claims should be construed as being included in the scope of the present invention.

A, A ': decanting kit, 1: centrifuge container
2: component recovery container, 11: first container body
11a: first chamber, 12: first container cover
21: second container body, 21a: second chamber
22: second container cover, 13, 13 ': plotter
121: outlet pipe portion, 122: injection port portion
122-1, 221-1: packing holder, 122-11, 221-11: insertion hole
122-12, 221-12: Barrier, 131, 131 ': Scaffold
132, 132 ': through hole, 132-1, 132-1': expansion part
133, 133 ': a barrier portion, 134:
211: inflow hole, 211-1: slope
221: extraction port portion, 222: vent port portion

Claims (7)

A first container body provided with a first chamber capable of containing a predetermined amount of a sample to be subjected to centrifugal separation and in which a sample is concentrated and separated at the time of centrifugal separation, and an upper end of the opening of the first container body, A first container lid capable of injecting a sample into the chamber and a second container lid provided in the first container main body and having a longitudinal direction of the first container body so as to be positioned at a boundary between the components based on density differences of components of the centrifuged sample A centrifuge container composed of a floater moving along the centrifuge; And
A second container body provided with a second chamber through which components flowing out from the centrifuge container are separated and moved, and a second container lid which hermetically closes the upper end of the opening of the second container main body and is capable of extracting components from the second chamber The first container body is assembled to one side of the second container body by being connected to an outlet pipe portion protruding from the first container lid so that a component having a lower density can flow from the first container body, Wherein a portion of the inflow stem adjacent to the second container body is configured to be gentle so that the cells of the inflow component due to the drop impact are not destroyed And a component collection container for separating and storing the components flowing out from the centrifugal separation container, the centrifugal separation container having an inclined surface, Wherein the composition is easy to separate and extract by components.
delete delete The method according to claim 1,
The floater has a sufficient gap with the bottom surface of the first container body so that a plurality of scaffolds, which have a high density of components densely downward from the first chamber during centrifugation, protrude from the bottom surface of the bottom plate ,
Wherein one or more through holes are formed through a predetermined portion of the bottom plate so as to pass through a component having a high density during centrifugation and settled to a lower side of the first chamber,
A barrier portion for preventing the backflow of the precipitate component is protruded and formed at the center of the upper surface of the bottom plate on the upper side of the through hole,
Wherein the inner surface of the upper end portion of the through hole is further provided with an expansion portion for smoothly discharging a component having a higher density.
The method according to claim 1,
The first container lid includes an outlet pipe portion connected to the ingredient recovery container and configured to allow a low-density component to flow out from the first container main body, which has become inclined horizontally due to centrifugal force during centrifugal separation, , An injection port portion for injecting a sample into the first chamber is protruded from a predetermined portion,
The second container lid is provided with an extraction port part for extracting the components contained in the second chamber and a second port part for connecting the sample to the outside of the first and second chambers using the injection port part and the extraction port part, Wherein a vent port for controlling entrance and exit is protruded from a predetermined site, respectively.
5. The method of claim 4,
The floater has a shape of a basket formed such that an outer surface thereof is connected to an upper side of a rim of the bottom plate, the outer surface of the float increases in diameter toward the upper side,
Wherein a protrusion is formed on an outer surface of the outer side of the outer surface so as to improve buoyancy by minimizing a contact friction with the inner surface of the first container body.
6. The method of claim 5,
Wherein the injection port portion and the extraction port portion are provided with a packing holder for preventing any leakage of the sample contained in the first chamber and the second chamber,
The packing holder is made of a flexible material such as rubber or silicone. The insertion hole through which the needle of the needle passes is formed along the longitudinal direction at the center. The needle can be inserted into the upper side of the insertion hole, And a blocking film is formed to prevent any leakage of the sample contained in the second chamber.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210053668A (en) 2019-11-04 2021-05-12 미라셀 주식회사 Decanting-kit container for divide extraction easy by ingredient specific
WO2022158618A1 (en) * 2021-01-25 2022-07-28 신현순 Decanting kit for enabling easy separation and extraction of components

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US4026433A (en) 1975-01-16 1977-05-31 Egidia Crippa Container provided with an outer testpiece for the analysis of urine and other acid liquids
JP5012610B2 (en) 2007-04-16 2012-08-29 ケテンバッハ ゲーエムベーハー ウント ツェーオー．カーゲー Container and method for opening a container
KR101197908B1 (en) 2011-10-31 2012-11-05 박현정 A container for centrifugal separation
KR101197974B1 (en) * 2011-11-01 2012-11-05 박현정 A container for centrifugal separator capable of rapid centrifugal separation

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Publication number Priority date Publication date Assignee Title
US4026433A (en) 1975-01-16 1977-05-31 Egidia Crippa Container provided with an outer testpiece for the analysis of urine and other acid liquids
JP5012610B2 (en) 2007-04-16 2012-08-29 ケテンバッハ ゲーエムベーハー ウント ツェーオー．カーゲー Container and method for opening a container
KR101197908B1 (en) 2011-10-31 2012-11-05 박현정 A container for centrifugal separation
KR101197974B1 (en) * 2011-11-01 2012-11-05 박현정 A container for centrifugal separator capable of rapid centrifugal separation

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210053668A (en) 2019-11-04 2021-05-12 미라셀 주식회사 Decanting-kit container for divide extraction easy by ingredient specific
KR102323471B1 (en) * 2019-11-04 2021-11-09 미라셀 주식회사 Decanting-kit container for divide extraction easy by ingredient specific
WO2022158618A1 (en) * 2021-01-25 2022-07-28 신현순 Decanting kit for enabling easy separation and extraction of components

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