KR101897979B1 - Centrifuge container for divide-extraction easy by ingredient specific - Google Patents

Centrifuge container for divide-extraction easy by ingredient specific Download PDF

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Publication number
KR101897979B1
KR101897979B1 KR1020170040571A KR20170040571A KR101897979B1 KR 101897979 B1 KR101897979 B1 KR 101897979B1 KR 1020170040571 A KR1020170040571 A KR 1020170040571A KR 20170040571 A KR20170040571 A KR 20170040571A KR 101897979 B1 KR101897979 B1 KR 101897979B1
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KR
South Korea
Prior art keywords
portion
sample
container
components
formed
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KR1020170040571A
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Korean (ko)
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신현순
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신현순
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021Test tubes specially adapted for centrifugation purposes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0689Sealing
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14Process control and prevention of errors
    • B01L2200/141Preventing contamination, tampering
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/04Closures and closing means
    • B01L2300/041Connecting closures to device or container
    • B01L2300/042Caps; Plugs
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/04Closures and closing means
    • B01L2300/046Function or devices integrated in the closure
    • B01L2300/047Additional chamber, reservoir
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/04Closures and closing means
    • B01L2300/046Function or devices integrated in the closure
    • B01L2300/048Function or devices integrated in the closure enabling gas exchange, e.g. vents

Abstract

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a centrifugal separator which is easy to separate and extract by components, and more particularly to a centrifugal separator for separating components of a sample such as blood or bone marrow using a centrifugal separator, The present invention relates to a centrifugal separator which is easy to separate and extract by components so that selective extraction of enriched (separated) components can be facilitated.
According to the present invention, there can be provided a container body which can contain a predetermined amount of a sample to be subjected to centrifugal separation, and a sample is concentrated and separated on a component basis during centrifugation; A container cover which seals the upper end of the opening of the container body and is capable of injecting the sample and selectively extracting a specific component of the components of the centrifuged sample; And a floating basket provided in the container main body and moving along the longitudinal direction of the container main body so as to be positioned at the boundary of the respective components based on the difference in density of each component during centrifugal separation.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a centrifugal separator,

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a centrifugal separator which is easy to separate and extract by components, and more particularly to a centrifugal separator for separating components of a sample such as blood or bone marrow using a centrifugal separator, The present invention relates to a centrifugal separator which is easy to separate and extract by components so that selective extraction of enriched (separated) components can be facilitated.

If suspended suspension with floating material is kept still, the denser material slowly sinks to the bottom due to the effect of gravity, and the denser material slowly moves to the upper layer. This process is called sedimentation (sediment).

Thus, when the materials with different densities are mixed, precipitation occurs, and over time, the mixture can be separated according to the density difference. In other words, the density difference between the mixtures increases as the gravity, which is the force for separating the mixture, becomes larger. Therefore, if the gravity is artificially increased, the precipitation phenomenon can be accelerated.

Therefore, it is easy to accelerate the precipitation phenomenon by using centrifugal force instead of gravity. This process is called centrifugation. A centrifugal separator (centrifugal separator) is a device used to separate, purify, and concentrate a substance having a different component or specific gravity (density difference) by using the principle of centrifugal separation described above. , Wastewater treatment, uranium enrichment, production, and laboratory use.

Among them, the medical centrifuge has a purpose of separating constituents for analysis of blood, urine, saliva, etc., but also for platelet-rich plasma extraction. Herein, the platelet-rich plasma (PRP) can be obtained by centrifugation of a complex fluid (hereinafter referred to as "sample") such as bone marrow or blood, and is highly enrichment , High concentration) Plasma components, which contain several growth factors, heal wound healing and reconstructs the damaged area. For example, it is used for reconstructing a damaged part by injecting a damaged part such as a ligament, a cartilage, etc., since it uses its own bone marrow or blood, so there is no side effect and treatment effect is fast.

On the other hand, the centrifugal separator described above is divided into various types according to the quantity of the sample to be centrifuged, the rotational speed, the type of the rotor, and the centrifugal force of the rotor rotational motion, And a centrifugal separator for performing centrifugal separation.

Based on this technical idea, Korean Patent Publication No. 10-2007-0026316 discloses a disposable container for centrifugation and treatment of a fluid biological material, The centrifugal component is sucked into a third canola (hose) which is connected to the bottom of the container by inserting a needle into the third canola (hose) chamber, the centrifuged components are mixed again due to the change in the sample content in the sample (in the sample fresh water drop) during the suction extraction (suction) using the syringe, So it is difficult to extract only specific ingredients.

In addition, since an adhesive is used for coupling between the lid and the container, it is difficult to assemble and manufacture the centrifuge container. In addition, since the sample injection port and the extraction port are formed of a rubber material, There is a problem that it is not easy to inject the sample or extract the separated components separately.

Korean Patent Publication No. 10-2007-0026316 (published date: 2007. 03. 08.)

The object of the present invention is to solve the above problems, and it is an object of the present invention to provide a centrifugal separator for separating a sample by centrifugal force and the specific gravity of each component, The present invention provides a centrifugal separator which is easy to separate and extract by components so that the separation of the separated components can be purified, and the extraction of the characteristic components from the separated components can be more complete, straightforward and quick.

Another object of the present invention is to provide a centrifugal separator which can realize an assembly coupling relation between a lid constituting a centrifuge container and a container body and an injection port and an extraction port for injecting a sample and extracting a characteristic component, It is, of course, an object of the present invention to provide a centrifugal separation container which can be separated and extracted easily so that the operation efficiency of a specific component extraction can be greatly improved.

In order to accomplish the above object, the present invention provides a container body, which can contain a predetermined amount of a sample to be subjected to centrifugal separation, and a sample is concentrated and separated by centrifugal separation; A container cover which seals the upper end of the opening of the container body and is capable of injecting the sample and selectively extracting a specific component of the components of the centrifuged sample; And a floating basket provided in the container main body and moving along the longitudinal direction of the container main body so as to be positioned at the boundary of the respective components based on the density difference of the components during centrifugal separation, And a flange portion extending in a circular plate shape at an upper end of the cylindrical portion and capable of correspondingly engaging with the container lid, wherein the tubular portion is biased toward one side of the flange portion And a weight for balancing movement of the center of gravity so that the center of gravity of the center of gravity of the tubular body is balanced, The present invention provides a centrifugal separator which is easy to separate and extract by components.

In addition, the container lid is provided with an injection port part for injecting the sample into the space inside the container body, an extraction port part for extracting a component contained in the space inside the container body, And a vent port portion for controlling air in and out is formed so as to protrude upward from a predetermined portion in combination with sample injection and extraction outside the container body.

delete

The float basket is provided with a plurality of foot plates protruding from the outer surface of the bottom plate so that sufficient clearance is formed between the bottom plate and the bottom of the barrel body, A through hole is formed at the center of the bottom plate so as to allow the component having a high density to pass through the centrifugal separation and settled to the lower side of the cylindrical member.

In addition, the outer surface of the floating basket is formed to have a larger diameter as it goes to the upper side and to be inclined toward the upper side when viewed in section, and the protrusion for reducing movement friction through minimization of the contact area with the inner surface of the cylindrical body protrudes from the upper end outer surface An undercut portion for smoothly distributing the settling component is formed inwardly at the center of the outer surface of the bottom plate where the end of the through hole is located .

In addition, the floating basket is formed with a barrel portion which is easy to deposit components through the through holes, but blocks the backflow of the precipitating component, at the center of the inner surface of the bottom plate.

In addition, the flange portion is provided with a first hook protruding upward from the rim portion in a state of being assembled with the container lid so as to protrude upward, and a gap between the container body and the container lid is blocked to prevent any leakage of the sample The first packing ring and the second packing ring are seated on the upper end.

The injection port portion and the extraction port portion are each provided with a packing holder for preventing any sample contained in the space inside the container body from leaking out. The upper portion of the packing holder is provided with a guider Respectively.

In addition, at the upper end of the through-hole, a bellows portion for smoothly discharging a component having a high density is formed.

In addition, a second hook extending toward the bottom of the container lid is formed so as to firmly assemble the container body and the container lid with each other in connection with the first hook.

In addition, the packing holder is made of a flexible material such as rubber or silicone, and an insertion hole through which the needle of the needle is passed is formed along the longitudinal direction at the center. On the upper side of the insertion hole, the needle can be inserted into the insertion hole, And is closed by forming a blocking plate to prevent any leakage of the sample contained in the body space.

As can be clearly understood from the above description, the centrifugal separating vessel of the present invention, which is easy to separate and extract according to each component of the present invention, can assure easiness of the construction of the centrifugal separating vessel by achieving the assembling structure of the lid and the container body by the simple fitting method, So that it is possible to precisely insert the needle into the desired position in a state of being guided by the extraction port and to improve the working efficiency such that the operation of injecting the sample into the state and the operation of extracting the ingredient can be performed easily and promptly.

In addition, in the process of extracting a specific component by the floating basket which floats in a state where it is placed at the boundary of the separation component according to the density difference of the components separated by the component in the inner space of the container body by centrifugation, It is not mixed with the extracted component, so that the extraction of the specific component is more completely performed.

In addition, according to achievement of various effects as described above, it is possible to completely separate the components from the blood or bone marrow used for autologous bone marrow therapy and autologous blood treatment, and the treatment using such separated components (platelet rich plasma, etc.) It is a very useful invention that can contribute to the development of the medical industry related to this and, in addition, it can achieve medical achievement such as improving the effect.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a perspective view showing the outer structure of a centrifugal separator according to the present invention, which facilitates separate extraction and extraction of components. FIG.
FIG. 2 is an exploded perspective view showing a constitutional relationship of a centrifugal separation container which can be easily separated and extracted according to the present invention. FIG.
FIGS. 3A to 3C are cross-sectional views illustrating the structure of a centrifugal separator according to the present invention that facilitates separate extraction and extraction of components. FIG.
FIG. 4 is a cross-sectional view illustrating the structure of a floating basket in a centrifugal separator container which can be easily separated and extracted according to the present invention. FIG.
5 (a) and 5 (b) are cross-sectional views showing other structures of a floating basket in a centrifugal separator container in which separation and extraction of components according to the present invention is easy.
FIG. 6 and FIG. 7 are usage diagrams showing examples of using centrifugal separation vessels which can be easily separated and extracted according to the present invention.
FIG. 8A and FIG. 8B are views showing operation examples of a centrifugal rotor showing an embodiment of a centrifugal separator which can be easily separated and extracted according to the present invention. FIG.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention. First, in adding reference numerals to the constituents of the drawings, it is to be noted that the same constituents are denoted by the same reference numerals as much as possible even if they are displayed on different drawings. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

FIG. 1 is a perspective view showing an outer structure of a centrifugal separator which is easy to separate and extract according to the present invention, and FIG. 2 is a perspective view showing a constitutional relationship of a centrifugal separator 3A to 3C are cross-sectional views illustrating the structure of a centrifugal separator according to an embodiment of the present invention. FIG. 4 is a cross-sectional view of the centrifugal separator of FIG. 5 (a) and 5 (b) are cross-sectional views showing another structure of a floating basket in a centrifugal separator which can be easily separated and extracted according to the present invention, and FIGS. 6 and 7 FIGS. 8A and 8B are views showing the state of use of the centrifugal separator according to the present invention. FIG. Fig. 6 is an operation example of a centrifuge rotor showing an example.

As shown in FIGS. 1 and 2, the centrifugal separator (A), which facilitates separate extraction and extraction of components according to the present invention, can contain a predetermined amount of a sample to be subjected to centrifugal separation, A vessel lid (2) capable of selectively extracting a specific component of the constituents of the injected and centrifuged sample by sealing the upper end of the opening of the vessel body (1) And a floating basket 3 provided in the container main body 1 and moving along the longitudinal direction of the container main body 1 so as to be located at the boundary of the respective components based on the density difference of the components during centrifugal separation.

Here, the centrifugal separator 900 is used for separating and recovering only specific components from a complex fluid (hereinafter referred to as a "sample") such as separation of blood cells from plasma or separation of stem cells from bone marrow 6, a rotor 910 which rotates horizontally when the apparatus is driven is installed inside, and the centrifuge vessel A of the present invention is accommodated in the rotor 910, A plurality of buckets 911 for performing a rotating movement when the rotor 910 rotates are provided.

7, the protruding end 911-1 for preventing the centrifuge vessel A from flowing when the rotor 910 rotates is horizontally protruded from the inner surface of the upper end of the bucket 911, And a through hole 911-2 through which the inside of the container body 1 can be seen is preferably penetrated.

One or more coupling grooves 911-21 for fitting the coupling protrusions projecting from the bottom surface of the flange portion of the centrifuge container A, which will be described later, are formed in the protruding end 911-1 desirable.

As shown in Figs. 2 and 3A, the container main body 1 can contain an appropriate amount of the sample, and when the centrifugation is performed, the sample has a density difference (11) having a chamber (111) capable of concentrating and separating by components by a centrifugal separator (11), and a cylindrical shape extending in an upper end of the cylindrical body (11) And a flange portion (12).

Here, as shown in Fig. 3A, the cylindrical portion 11 is biased to one side rather than the center of the flange portion 12. As shown in Fig. The center of gravity of the cylindrical body 11 is balanced with the balance of the center of gravity of the cylindrical body 11 at the lower side of the large area of the flange portion 12, And a weight portion 121 for balanced movement of the center of gravity so that the swiveling movement is performed in a state of being inclined close to the horizontal with the swivel base 911. Thus, the efficiency of separation (centrifugation) of the sample due to the centrifugal centrifugal force of the rotor 910 during the operation of the centrifugal separator 900 can be greatly improved.

The flange portion 12 of the other side of the weight portion 121 is in contact with the protruding end 911-1 of the bucket 911 and the coupling groove 911-11, It is preferable that a plurality of coupling projections 122, which can be inserted into the recesses, are formed at predetermined positions.

The flange portion 12 is provided with a first hook 123 protruding from the top of the rim for easy assembly with the container lid 2 and firmly assembling the container lid 2, A protruding frame 124 that is fitted to the inside of the second projection of the container lid 2 to be described later is protruded upward.

In addition, a first packing ring 125 and a second packing ring 126 are provided at the upper end in order to block the gap between the container main body 1 and the container lid 2 to prevent any leakage of the sample.

The container lid 2 is assembled on the container main body 1 so as to close the upper end of the opening of the cylindrical body part 11 while being injected into the chamber 111, The first hook 123 is formed to have a circular plate-like structure capable of covering the flange portion 12 of the container main body 1, And a second projection 22 for receiving the inside of the pawl body 124 is formed from a portion corresponding to the pawl body 124 as described above and a first projection 21 for projecting the projection As shown in FIG.

As shown in FIGS. 3A to 3C, the injection port portion 23 for injecting the sample into the internal space (chamber 111) of the cylindrical body portion 11 and the injection port portion 23 for extracting the components from the chamber 111 (Injection and extraction) of a fluid biological material (hereinafter referred to as "sample") out of the container body 1 using the injection port portion 23 and the extraction port portion 24 The air vent port portion 5 is formed so as to protrude upward from a predetermined portion.

In addition, a packing holder 26 for preventing any leakage of the sample contained in the chamber 111 is contained in the injection port portion 23 and the extraction port portion 24, And a guider 27 for guiding insertion of the needle into the upper side is further coupled.

The flange 12 of the container body 1 is wrapped around the outward vertical end of the first protrusion 21 and the container body 1 is fastened to the first hook 123 of the flange portion 12, A second hook 211 is formed extending downward so that mutual assembly of the container lid 2 and the container lid 2 is made firm.

The inner surface of the second protruding portion 22 is in contact with the inner wall surface of the cylindrical portion 11 of the container main body 1 so as to improve the assemblability between the container main body 1 and the container lid 2, (221) extending downward.

The packing holder 26 is made of a flexible material such as rubber or silicone and has an insertion hole 261 through which the needle of the needle passes and is formed at the center along the longitudinal direction. The needle can be pierced while being closed by the formation of a blocking plate 262 which prevents any leakage of the sample injected into the chamber 111. [

The floating basket 3 is provided to prevent the sedimented component and the suspended component from being mixed with each other through the boundaries of the components of the sample concentrated and separated in the container body 1 by the components, Similarly, a structure similar to that of a cup having an open upper end is formed with a sufficient clearance with the bottom surface of the cylindrical body part 11, so that a precipitate of a dense component downwardly of the cylindrical body part 11 during centrifugal separation A plurality of foot plates 31 protruding from the outer surface (bottom surface) of the bottom plate are formed in the center of the bottom plate so as to allow components having a high density to pass through the center of the bottom plate, 32 are formed through.

At the central portion of the inner surface (upper surface) of the bottom plate on which the through holes 32 are formed, a barrier portion 33 for easily precipitating the components through the through holes 32 but blocking the back flow of the precipitating components is protruded And an expanded portion 321 is formed at the upper end of the through hole 32 to facilitate discharge of a component having a high density.

In addition, the outer surface is formed so as to be inclined upward toward the upper side as viewed in cross section, and has a protruding portion 34 for improving buoyancy by minimizing the contact friction with the inner surface of the cylindrical portion 11, And may be formed so as to protrude from the outer surface of the image end.

5 (a) and 5 (b), the structure of the floating basket 3 can be variously changed. As shown in FIG. 5 (a) A cutting surface 35 for further improvement can be further formed on the inner surface of the upper end. In addition, the central portion of the outer surface (bottom surface) of the bottom plate serving as the end of the through hole 32 is recessed, The undercut portion 36 for guiding can be formed further inward.

Hereinafter, the operation of the centrifugal separator (A) having the above-described structure and facilitating the separation and extraction of components according to the present invention will be described in detail.

First, the sample contained in the syringe is transferred to the centrifuge container 1 for separation of components of the sample such as blood or bone marrow collected from the patient by using a syringe.

The injection port portion 23 of the container lid 2 is injected into the cylindrical portion 11 of the container body 1 and the guider 27 are inserted into the guide tube portion 271 and inserted into the packing hole of the packing hole 26 so that the tip of the needle reaches the chamber 111.

Then, when the sample is injected into the cylindrical portion 11 according to such a procedure, the air staying in the chamber 111 is discharged through the air port portion 25, so that the internal pressure of the cylindrical portion 11 is balanced Can be maintained.

6 to 8A, the centrifuge vessel A of the present invention is accommodated in each of the buckets 911 hinged to the rotor 910 of the centrifugal separator 900, The separator 900 is driven so that the rotor 910 is horizontally rotated to perform a centrifugal separation process in which constituent components of the sample contained in the cylindrical portion 11 of the container body 1 are separated, It is possible that the constituents have different densities.

That is, as shown in FIG. 8B, the centrifuge vessel A of the present invention, which is accommodated and mounted in the bucket 911 due to the centrifugal force generated during horizontal rotation of the rotor 910, Whereby a high density component of the sample contained in the inner space (chamber 111) of the container body 1 of the centrifugal separation container A is settled in the lower layer portion with the bucket 911 , And a component with a low density floats to the upper layer.

For example, in the case of blood, a blood component, which is a high density component, is concentrated and separated in the lower part of the chamber 111 of the cylindrical part 11 of the container body 1, and a plasma having a low density is concentrated and separated in the upper part.

During this process, the floating basket 3 moves (floats) in the chamber 111 along the longitudinal direction of the cylindrical portion 11 due to the difference in density of the components during the separation of the components by the component, Component is discharged through the through hole 32 penetrating through the bottom plate of the floating basket 3 and is discharged to the lower portion of the chamber 111, It is possible by influence.

At this time, since the outer surface of the bottom plate of the floating basket 3 is not completely adhered to the bottom surface of the cylindrical body 11 by the foot plate 31, the high density component passes through the through hole 32 So that sedimentation can be performed on the bottom side of the cylindrical body part 11. [

In addition, the high density component can be smoothly discharged through the through hole 32 by the formation of the expanded portion 321, and the flow of the component that is discharged to the lower portion of the chamber 111 is discharged to the undercut portion 36 And can be smoothly moved by the reduction of the movement friction due to the state in which the contact area with the inner surface of the cylindrical portion 11 is minimized by the protruding portion 34. [

Subsequently, the centrifugal separation process is carried out for a predetermined time to selectively extract a specific component of the components of the sample separated by the component, The tip of the needle penetrates the blocking film 262 of the packing holder 26 and reaches the chamber 111 through the insertion hole 261 of the packing holder 26 It is possible to insert it sufficiently.

In this case, it is possible to extract a specific component of the components of the sample separated by the components in the chamber 111 of the cylindrical body part 11. In this case, The internal pressure of the cylindrical body portion 11 can be balancedly maintained by being introduced into the main body 1.

In this state, when the amount of fresh water in the chamber 111 changes in the process of selectively extracting a specific component, components having different densities can be mixed based on the operation, Is prevented by the floating basket (3).

Thus, the platelet-rich plasma can be extracted, and the platelet-rich plasma thus extracted is applied or injected into the lesion of the patient (damaged skin, muscle, ligament, cartilage, etc.) Can be used for autologous bone marrow or autologous blood treatment.

According to the present invention, the centrifugal separation vessel (A), which is easy to separate and extract according to the components of the present invention, facilitates centrifugal separation of components of the sample such as blood or bone marrow, It becomes easier to selectively extract a specific component from the chamber 111 from the sample injection into the inner space (chamber 111) and centrifuged sample components.

Further, the problem that the already separated components are mixed due to the change in the sample storage amount in the process of extracting the specific component from the chamber 111 makes it impossible to complete the specific component, There is an effect that mixing of the components is prevented by the floating basket 3 to be positioned.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention as defined by the appended claims. will be. Therefore, the embodiments disclosed in the present invention and the accompanying drawings are intended to illustrate and not to limit the technical spirit of the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments and the accompanying drawings . The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of the claims should be construed as being included in the scope of the present invention.

A: centrifuge vessel, 1: container body, 2: container lid
3: floating basket, 11: cylindrical portion, 12: flange portion
21: first projecting portion, 22: second projecting portion, 23: injection port portion
24: extraction port portion, 25: vent port portion, 26: packing holder
27: guider, 31: footstool, 32: passage
33: barrier portion, 34: projection portion, 35: cutting face
36: undercut portion, 111: chamber, 121: weight portion
122: a coupling protrusion, 123: a first hook, 124: a projection
125: first packing ring, 126: second packing ring, 211: second hook
221: holding frame, 261: insertion hole, 262:
321:

Claims (11)

  1. A container body which can contain a predetermined amount of a sample to be subjected to centrifugal separation, and a sample is concentrated and separated by centrifugation;
    A container cover which seals the upper end of the opening of the container body and is capable of injecting the sample and selectively extracting a specific component of the components of the centrifuged sample; And
    And a floating basket provided in the container main body and moving along the longitudinal direction of the container main body so as to be positioned at the boundary of the respective components based on the difference in density of each component during centrifugal separation,

    Wherein the container body includes a cylindrical cylindrical portion having a chamber and a flange portion formed in a disk shape on an upper end of the cylindrical portion and capable of correspondingly coupling with the container lid,
    The tubular portion is biased to one side rather than the center of the flange portion,
    A weight portion for balanced movement of the center of gravity for pivotal movement in a state of being tilted horizontally at the time of centrifugal separation is formed on the lower side of a portion having a large area of the flange portion in addition to balancing of the center of gravity Which is easy to separate and extract by components.
  2. delete
  3. The method according to claim 1,
    The container lid is provided with an injection port for injecting a sample into the space inside the container body,
    An extraction port for extracting a component contained in the space inside the container body,
    Wherein a vent port portion for controlling air in and out is formed so as to protrude upward from a predetermined portion in combination with sample injection and extraction outside the container body using the injection port portion and the extraction port portion. One centrifuge vessel.
  4. The method according to claim 1,
    The floating basket is formed with a plurality of scaffolds which are sufficiently free from settling of components having a high density below the cylindrical body portion during centrifugal separation so as to form a sufficient gap with the bottom surface of the cylindrical body portion,
    Wherein a through hole is formed at the center of the bottom plate so that a component having a high density passes through the centrifugal separation and can be precipitated to the lower side of the container body.
  5. 5. The method of claim 4,
    The outer surface of the floating basket is formed such that its diameter becomes larger toward the upper portion and is inclined toward the upper portion when viewed in section,
    A projecting portion is formed on an outer surface of an upper end for reducing movement friction by minimizing a contact area with the inner surface of the cylindrical portion,
    A cutting surface is formed inwardly in the upper end inner surface so as to improve buoyancy by minimizing self-load,
    Wherein an undercut portion for smoothly distributing a settling component is formed inwardly at a central portion of an outer surface of a bottom plate where the end of the through hole is located.
  6. 5. The method of claim 4,
    Wherein the floating basket is formed with a barrier part for easily precipitating the components through the through hole while blocking the backflow of the precipitating component, and protruding from the central part of the inner surface of the bottom plate.
  7. The method according to claim 1,
    Wherein the flange portion is provided with a first hook protruding upward from a rim portion in a state of being assembled with the container lid,
    Wherein a first packing ring and a second packing ring for preventing any leakage of the sample by blocking a gap between the container body and the container lid are seated on the upper end thereof.
  8. The method of claim 3,
    Wherein the injection port portion and the extraction port portion are respectively provided with a packing holder for preventing any leakage of the sample contained in the space inside the container body,
    And a guider for guiding insertion of the needle into the upper portion of the packing holder is coupled to the upper portion of the packing holder.
  9. 5. The method of claim 4,
    Wherein an upper portion of the through hole is formed with an expanded portion for facilitating the discharge of a component having a higher density.
  10. 8. The method of claim 7,
    And a second hook extending in a downward direction is formed at the rim of the container lid so as to firmly assemble the container body and the container lid with a corresponding binding with the first hook. Centrifuge container.
  11. 9. The method of claim 8,
    The packing holder is made of a flexible material such as rubber or silicone,
    An insertion hole through which the needle is passed is formed along the longitudinal direction at the center,
    Wherein an upper portion of the insertion hole is in an airtight state by forming a blocking plate which can penetrate the needle but can prevent any leakage of the sample contained in the inner space of the cylindrical portion. Vessel.
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006502388A (en) * 2002-10-03 2006-01-19 バッテル メモリアル インスティチュート Buffy coat separation float system and method
KR20070026316A (en) 2003-10-10 2007-03-08 아지엔드 키미쉐 리유나이트 안젤리니 프란체스코 에이.씨.알.에이.에프. 에스.피.에이 Disposable container for centrifuging and treating a fluid biological material
WO2013066014A1 (en) * 2011-11-01 2013-05-10 Park Hyun Jeong Container for centrifugal separation providing rapid centrifugal separation
WO2013066013A1 (en) * 2011-10-31 2013-05-10 Park Hyun Jeong Container for centrifugal separation
JP2014513630A (en) * 2011-03-02 2014-06-05 レアサイト インコーポレイテッドRareCyte,Inc. Tube and float systems for separating fluids on a concentration basis
JP2014532874A (en) * 2011-11-08 2014-12-08 レアサイト インコーポレイテッドRareCyte,Inc. Method and system for separating components of a suspension using a secondary liquid

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006502388A (en) * 2002-10-03 2006-01-19 バッテル メモリアル インスティチュート Buffy coat separation float system and method
KR20070026316A (en) 2003-10-10 2007-03-08 아지엔드 키미쉐 리유나이트 안젤리니 프란체스코 에이.씨.알.에이.에프. 에스.피.에이 Disposable container for centrifuging and treating a fluid biological material
JP2014513630A (en) * 2011-03-02 2014-06-05 レアサイト インコーポレイテッドRareCyte,Inc. Tube and float systems for separating fluids on a concentration basis
WO2013066013A1 (en) * 2011-10-31 2013-05-10 Park Hyun Jeong Container for centrifugal separation
WO2013066014A1 (en) * 2011-11-01 2013-05-10 Park Hyun Jeong Container for centrifugal separation providing rapid centrifugal separation
JP2014532874A (en) * 2011-11-08 2014-12-08 レアサイト インコーポレイテッドRareCyte,Inc. Method and system for separating components of a suspension using a secondary liquid

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