KR102316718B1 - Manufacturing method for grains comprising the mycelium of mushrooms using a liquid medium - Google Patents

Abstract

Disclosed is a method for manufacturing grains comprising mycelium of mushrooms using a liquid medium, capable of liquid-germinating medicinal mushrooms in the grains, inoculating the same, significantly reducing the number of incubation days, and inducing even culture. The method of the present invention comprises: a step of clearly washing the grains from which foreign substances are filtered and immersing the grains in water; a step of putting the grains on a strainer tray after completing immersion and removing moisture; a step of inserting the grains from which the moisture is removed into a container and closing a cover having a filter; a step of treating high-pressure sterilization of the grains in the container; a step of naturally discharging the sterilized container and cooling the sterilized container at the same time; a step of inoculating a medicinal mushroom liquid germ culture medium composition into the grains in the container after completing cooling; a step of culturing medicinal mushroom mycelium on surfaces of the grains after completing inoculation; and a step of loosening lumps of the grains after completing the culture and drying the lumps with hot air.

Description

The method of manufacturing grains comprising the mycelium of mushrooms using a liquid medium {Manufacturing method for grains comprising the mycelium of mushrooms using a liquid medium}

The present invention relates to a method for producing grains containing the mycelium of mushrooms using a liquid medium, and more particularly, to a liquid that can induce an even culture while remarkably reducing the number of culture days by inoculating medicinal mushrooms by liquid spawning them on grains. It relates to a method for producing grains comprising the mycelium of mushrooms using a medium.

Mushrooms (fungi, 茸類) is a generic term for higher fungi belonging to the basidiomycetes. These mushrooms are classified into basidiomycetes (擔子菌類) and ascomycetes (子囊菌類) according to the production method of spores made for propagation. Most edible mushrooms belong to basidiomycetes.

There are thousands of known types of mushrooms, but it is known that about 180 species are currently edible.

Edible mushrooms have a unique flavor and texture, so many types of mushrooms are cultivated, but most mushrooms are not grown and are collected and sold in nature. Natural mushrooms that cannot be cultivated include matsutake, chestnut, shiitake, and truffle, and cultivated mushrooms include shiitake, oyster mushroom, wood ear mushroom, and oyster mushroom. Also, among mushrooms, there are medicinal mushrooms that are used for medicinal purposes according to their specific ingredients, and these medicinal mushrooms include reishi mushroom and the like.

The general components of these mushrooms are similar to vegetables and are characterized by a high content of ergosterol, which is provitamin D2. However, mushrooms are prone to deterioration because there are many enzymes. Therefore, it is common to store them dry or salted. Mushrooms are highly palatable foods with a unique flavor (scent, taste). In addition, shiitake mushrooms and mushrooms contain ingredients with special physiological effects.

In particular, mushrooms are a high-protein food and are known to have anticancer effects according to various research reports.

Mushrooms with such anti-cancer action include reishi mushroom and sanghao mushroom. Reishi mushroom has medicinal effects and is rare enough to be called bullocho from the old days. Due to the development of cultivation technology, it is well known for being used as a variety of health food as it is mass-produced. On the other hand, Sanghwang mushroom is not well known to the general public because it grows only on old mulberry trees, and its cultivation method has not been studied much, so it has not received much attention. There has been a lot of research and a lot of interest.

Various media are used for the cultivation of these mushrooms, but most of the media are simply manufactured from wood, pulverized wood or sawdust, and are discarded after use is complete. However, discarded medium contains a large amount of mycelium of mushrooms, and since this mycelium has the same components as the fruiting body used for food, efforts to use the mycelium even after collecting the fruiting body by replacing the medium with an edible medium This continues.

In particular, there is a conventional technique for culturing mycelium on rice using mushrooms, but it is difficult and takes a long time to cover the mycelium of a solid component on the rice surface with a thin film, so the utility value was not high.

Therefore, there is a need for a new method for culturing the mycelium of these mushrooms in an edible medium, especially on the surface of rice.

(0001) Republic of Korea Patent No. 10-0462497 (0002) Republic of Korea Patent Publication No. 10-2021-0036682

The present invention has been devised to solve the above problems, and an object of the present invention is to use a liquid medium for culturing mycelium on the surface of rice using a liquid spawn containing mycelium of mushrooms. Cereals containing mycelium of mushrooms To provide a manufacturing method.

Another object of the present invention is to provide a method for producing cereals containing mycelium of mushrooms using a liquid medium capable of reducing the number of culture days by using a liquid spawn.

Another object of the present invention is to provide a method for producing grains containing mycelium of mushrooms using a liquid medium capable of culturing mycelium evenly on the surface of rice by using a liquid spawn.

The problems of the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

In order to solve the above problems, the present invention includes the steps of washing the grains from which foreign substances have been filtered, and immersing them in water; After the immersion is completed, placing the grains on a tray and removing the water; loading the grains from which the water has been removed into a container and then closing a stopper having a filter; high-pressure sterilization of the grains in the container; Naturally evacuating the sterilized container and cooling it at the same time; inoculating the medicinal mushroom liquid seed medium composition into the grains in the container after the cooling is completed; culturing medicinal mushroom mycelium on the surface of cereals after the inoculation is completed; And it provides a method for producing cereals, including the mycelium of mushrooms using a liquid medium, comprising the steps of loosening the agglomerated portion of the grains after the culture is completed, and drying with hot air.

In one embodiment, the step of the high-pressure sterilization (sterilization) treatment, the primary sterilization step of heating the grain in the container to 101 ~ 105 ℃ 30 ~ 40 minutes; and a second sterilization step of heating the grains at 120 to 125° C. for 30 to 40 minutes after the first sterilization step is completed.

In one embodiment, the medicinal mushroom liquid seed culture medium composition comprises 0.05 to 0.07 parts by weight of peptone powder relative to 100 parts by weight of water, 0.04 to 0.06 parts by weight of malt extract powder, yeast extract powder ( Yeast extract Powder) 0.05 to 0.06 parts by weight, dextrose (dextrose) 0.5 to 0.8 parts by weight, and soybean (soybean Powder) may be a mixture of 0.05 to 0.2 parts by weight.

In one embodiment, the medicinal mushroom liquid seed medium composition is, peptone powder (peptone Powder), malt extract powder (malt extract Powder), yeast extract powder (yeast extract Powder), dextrose (dextrose) and soybean (soybean) Powder) into a tissue grinder, and pulverizing for 2-3 minutes; mixing the pulverized mixture with distilled water to prepare a liquid seed medium composition; and inoculating a medicinal mushroom seed into the liquid seed medium composition and culturing for 1 to 30 hours, wherein the medicinal mushroom is selected from the group consisting of Cordyceps, Shanghai mushroom, Reishi mushroom, Horseshoe mushroom and Chaga mushroom The culturing step may be a step of culturing while homogenizing the liquid seed medium composition inoculated with medicinal mushroom spawn using a magnetic bar and a magnetic stiller.

In one embodiment, the medicinal mushroom spawn, preparing a pulverized tree comprising at least one selected from the group consisting of pine, fir, oak, chestnut and acacia; Sterilizing the pulverized wood at a temperature of 100 to 150° C. for 50 to 100 minutes; A water supply step of making the water content of the sterilized pulverized wood to be 30 to 40% by weight; inoculating the mycelium of Sanghwang mushroom, chaga mushroom, horseshoe mushroom and reishi mushroom into the water-supplied pulverized water; Culturing the mycelium of the situation mushroom, chaga mushroom and reishi mushroom at a temperature of 20-25° C. for 20-40 days after inoculation is completed; obtaining a fruiting body after the culturing is completed; mixing 5 to 10 parts by weight of Cordyceps Cordyceps relative to 100 parts by weight of the obtained fruiting body; and drying the obtained mixture of fruiting bodies and Cordyceps cordyceps, and preparing medicinal mushroom spawns.

In one embodiment, the container is a container in which a part or all of the wall surface is made of a film, and the step of inoculating the medicinal mushroom liquid spawn medium composition to the grains in the container comprises: charging; penetrating the needle-shaped nozzle formed on one side of the injector through the film constituting the wall surface of the container so that the nozzle is located inside the container; spraying the medicinal mushroom liquid seed medium composition through a needle-type nozzle inside the container by pressing the injector; and removing the nozzle and then closing the through hole formed by the nozzle, wherein the needle-shaped nozzle has 1 to 5 injection holes, and the medicinal mushroom liquid spawn medium composition is placed in the container from 2 to It can be vaccinated 4 times.

The present invention also provides cereals comprising the mycelium of mushrooms prepared by the above method.

According to an embodiment of the present invention, a method for producing cereals containing mycelium of mushrooms, which can significantly reduce the number of culture days compared to the conventional method, by spraying a liquid seed produced using medicinal mushrooms on the surface of grain oil. can provide

In addition, the present invention can provide a method for producing cereals including the mycelium of mushrooms, which can be uniformly applied to the surface of the grain by using a liquid seed, so that the content of the mycelium can be increased.

In addition, the present invention provides a method for producing cereals containing the mycelium of medicinal mushrooms that allows consumers to consume the mycelium of medicinal mushrooms through a daily meal process by consumers. can

The effect according to the present invention is not limited by the contents exemplified above, and more various effects are included in the present invention.

1 schematically shows a method for producing cereals including mycelium of mushrooms according to an embodiment of the present invention.
2 is a photograph of cereals loaded in a container according to an embodiment of the present invention.
Figure 3 shows the structure of the tissue grinder according to an embodiment of the present invention.
Figure 4 shows the combination of the tissue grinder according to an embodiment of the present invention.
5 is a view showing the culture of the liquid medium according to an embodiment of the present invention.
6 is a photograph comparing brown rice with mycelium and non-formed brown rice according to an embodiment of the present invention.
7 shows an apolitoxin test result according to an embodiment of the present invention.
8 is a diagram showing the results of a junggeumsuk test according to an embodiment of the present invention.
9 shows the test results of Escherichia coli according to an embodiment of the present invention.
10 shows the residual pesticide test results according to an embodiment of the present invention.

Hereinafter, various embodiments will be described in more detail with reference to the accompanying drawings. The embodiments described herein may be variously modified. Certain embodiments may be depicted in the drawings and described in detail in the detailed description. However, the specific embodiments disclosed in the accompanying drawings are only for easy understanding of various embodiments. Therefore, the technical spirit is not limited by the specific embodiments disclosed in the accompanying drawings, and it should be understood to include all equivalents or substitutes included in the spirit and scope of the invention.

Terms including an ordinal number, such as first, second, etc., may be used to describe various elements, but these elements are not limited by the above-described terms. The above terminology is used only for the purpose of distinguishing one component from another component.

In this specification, terms such as "comprises" or "have" are intended to designate that the features, numbers, steps, operations, components, parts, or combinations thereof described in the specification exist, but one or more other features It should be understood that this does not preclude the existence or addition of numbers, steps, operations, components, parts, or combinations thereof. When a component is referred to as being “connected” or “connected” to another component, it is understood that the other component may be directly connected or connected to the other component, but other components may exist in between. it should be On the other hand, when it is said that a certain element is "directly connected" or "directly connected" to another element, it should be understood that no other element is present in the middle.

Meanwhile, as used herein, a “module” or “unit” for a component performs at least one function or operation. And “module” or “unit” may perform a function or operation by hardware, software, or a combination of hardware and software. In addition, a plurality of “modules” or a plurality of “units” other than a “module” or “unit” that must be performed in specific hardware or are executed in at least one processor may be integrated into at least one module. The singular expression includes the plural expression unless the context clearly dictates otherwise.

In addition, in describing the present invention, when it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the gist of the present invention, the detailed description thereof will be abbreviated or omitted.

1 shows a method for producing cereals including mycelium of mushrooms according to an embodiment of the present invention.

The present invention includes the steps of cleaning the grains filtered out of foreign substances, immersing them in water; After the immersion is completed, placing the grains on a tray and removing the water; loading the grains from which the water has been removed into a container and then closing a stopper having a filter; high-pressure sterilization of the grains in the container; Naturally evacuating the sterilized container and cooling it at the same time; inoculating the medicinal mushroom liquid seed medium composition into the grains in the container after the cooling is completed; culturing medicinal mushroom mycelium on the surface of cereals after the inoculation is completed; And it relates to a grain production method comprising the mycelium of mushrooms using a liquid medium comprising the steps of loosening the agglomerated portion of the grain after the culture is completed, and drying with hot air.

The grain is used as a predetermined culture medium in which the mycelium of medicinal mushrooms to be described later is cultured on the surface, and any grains that are consumed for food can be used without limitation. However, preferably, brown rice may be used in order to facilitate the cultivation of the medicinal mushroom and increase the texture at the same time.

The brown rice refers to rice that is milled to remove the outer hull and then the rice bran is not removed, and the mycelium has a rough surface, so the growth of the mycelium is easy. This is possible. In addition, in the case of general brown rice, it has a disadvantage of poor texture due to the rice bran, but in the present invention, as the rice bran is used to grow the mycelium of the medicinal mushroom, the texture becomes similar to white rice, so it is easy to consume the brown rice It has the advantage of being able to do it.

The grain may be washed with water and then immersed in water after foreign matter is filtered. In general, in the case of cereals, contamination may occur in the production and distribution process, and microorganisms may be mixed in the contamination process as described above. In the case of this mixed microorganism, it is preferable to remove the saline solution by washing with water because it may cause a substrate competition with the medicinal mushroom of the later sulphate or interfere with the growth of the medicinal mushroom. In this case, the water washing is preferably performed using water, and for the sterilization effect of microorganisms, water washing with ethanol may be concurrently performed. Preferably, water washing with water is performed 2-3 times, and then water washing with ethanol is performed once. The contaminants are physically removed using water and sterilization can be performed through ethanol washing at the same time.

After the washing is completed as described above, the grains may be immersed in water. In general, for the convenience of storage, grains may be harvested and then dried and distributed. However, in the present invention, since it is used as a medium for medicinal mushrooms, it is preferable to immerse the grains in water to have a certain amount of moisture. The immersion is preferably carried out for 10 to 12 hours at a temperature of 10 to 20 ℃. When the immersion is carried out at a temperature of less than 10 ° C or less than 10 hours, the moisture absorption may decrease and the mycelium growth of medicinal mushrooms may be difficult, and when immersed at a temperature exceeding 20 ° C or for a time exceeding 12 hours, the grains are It may become degenerate or microorganisms may multiply.

After the immersion is completed as described above, the grains may be placed on a tray to remove moisture. In this case, the tray bowl refers to a container in which a plurality of through holes smaller than the size of the grains are formed. When the grain is placed on the tray tray, excess water can be easily removed through the through holes.

The grains from which the excess water has been removed as described above may be charged into the container, and then the container may be closed using a plug with a filter. In this case, the container can be used without limitation as long as it has a structure that can contain the grains inside, but it is preferable to use a container in which a part or all of the wall surface is made of a polymer film for the injection process to be described later. In particular, in the case of a container in which two transparent polymer films are superimposed and attached to the rim, it is easy to check the growth state of the mycelium inside, and it is most preferable to use this because it is easy to inject through the wall. In addition, the polymer film may be used without limitation as long as it is a transparent material, but preferably polyvinyl chloride, polyethylene, polypropylene, polyethylene terephthalate or polycarbonate may be used.

The stopper is used to perform a disinfection process to be described later while closing the container, and as it includes a filter, it is possible to prevent the movement of microorganisms. In this case, the filter may have a pore size capable of exchanging air and water vapor while preventing the movement of microorganisms, through which heated air and water vapor can be injected into the container during the disinfection process, In the process of culturing the mushroom mycelium, the gas required for mushroom growth can be exchanged.

After the grain is charged into the container as described above, the grain in the container can be autoclaved (sterilization) (see FIG. 2).

At this time, the step of the high-pressure sterilization (sterilization) treatment, the primary sterilization step of heating the grain in the container to 101 ~ 105 ℃ 30 ~ 40 minutes; and a second sterilization step of heating the grains at 120 to 125° C. for 30 to 40 minutes after the first sterilization step is completed.

Heat sterilization generally used can kill most microorganisms, but it is known that some heat-resistant microorganisms cannot be killed. In particular, mold spores are known to be resistant to high heat. In the present invention, if the spores of the fungus as described above remain, it is preferable to perform autoclaving to kill the mycelium of medicinal mushrooms and substrate competition, thereby inhibiting growth.

At this time, the heating is a primary sterilization step of heating at 101 ~ 105 ℃ 30 ~ 40 minutes; And it is preferable to perform the second step roll sterilization of the second sterilization step of heating the grains at 120 to 125° C. for 30 to 40 minutes after the first sterilization step is completed. In the case of autoclaving used in the past, it is sterilized by simply heating at a temperature of 120 to 125° C., but in this case, some microorganisms form heat-resistant cysts or the grains may be contaminated due to toxic substances generated during sterilization. Therefore, in the case of the present invention, the formation of cysts can be inhibited by performing heating in two steps as described above, and the release of toxins from microorganisms can be minimized. In addition, in the case of the autoclaving, it is preferable to insert the container containing the grains into the autoclave, but supply high-temperature and high-pressure steam through the stopper to create a high-temperature and high-pressure sterilization environment for only the inside of the container. It is also possible to sterilize it. However, in this case, it is preferable to use the container and the stopper manufactured to be used in a high-pressure environment.

In addition, at this time, the autoclaving process may be performed by supplying steam pressurized at 15 psi.

After the high pressure sterilization is completed as described above, the container can be naturally evacuated and cooled at the same time. In the case of the container, although it is closed by the stopper, internal air may be circulated with the outside by a filter installed in the stopper. Therefore, when the container is withdrawn to the upper part after the autoclaving is completed, the pressure in the container may drop and the container may be cooled at the same time. At this time, the cooling process is preferably cooled to 20 ~ 40 ℃ similar to room temperature. In the case of cooling to less than 20℃, the growth of medicinal mushrooms, which will be described later, may be slowed, and if it exceeds 40℃, the mycelium of medicinal mushrooms may be killed due to high temperature.

After the cooling is completed as described above, the medicinal mushroom liquid seed medium composition may be inoculated into the grains in the container.

The medicinal mushroom liquid seed culture medium composition is a mixture in which the medicinal mushroom spawn is cultured in a liquid medium, and can be easily attached to the surface of the grains compared to a conventional solid medium.

To this end, the medicinal mushroom liquid seed culture medium composition contains 0.05 to 0.07 parts by weight of peptone powder, 0.04 to 0.06 parts by weight of malt extract powder, yeast extract powder relative to 100 parts by weight of water. 0.05 to 0.06 parts by weight, 0.5 to 0.8 parts by weight of dextrose, and 0.05 to 0.2 parts by weight of soybean Powder may be a mixture. That is, the medicinal mushroom liquid starter medium composition is a form of mixing water with a fungus 　 medium called PDB medium (Potato Dextrose Broth), and it can be used as a medicinal mushroom liquid starter medium composition by inoculating the medicinal mushroom seed into this medium (Fig. 5).

The medicinal mushroom liquid seed culture medium composition is a mixture of Peptone Powder, Malt Extract Powder, Yeast Extract Powder, Dextrose and Soybean Powder. Put in a grinder and grind for 2-3 minutes; mixing the pulverized mixture with distilled water to prepare a liquid seed medium composition; and inoculating the medicinal mushroom seed into the liquid seed medium composition and culturing for 1 to 30 hours.

A mixture of peptone Powder, malt extract Powder, yeast extract Powder, dextrose and soybean Powder is sold under the product name PD Broth, and this In the case of the invention, 0.05 to 0.07 parts by weight of peptone powder, 0.04 to 0.06 parts by weight of malt extract powder, 0.05 to 0.06 parts by weight of yeast extract powder, dextro It is preferable to use a mixed medium in a ratio of 0.5 to 0.8 parts by weight of dextrose and 0.05 to 0.2 parts by weight of soybean Powder. When using a mixture having a composition within the above range, it is easy to culture the mycelium of the present invention, but if it is outside the above range, it may be difficult to culture.

The medium mixture may be charged into a tissue grinder and pulverized for 2-3 minutes. Through this tissue grinder, the medium can be crushed into fine tissues so that it can be dissolved or dispersed in water, and the crushed medium mixture can be mixed with distilled water and used as a liquid medium. At this time, the grinding may be performed for 2-3 minutes, and when the grinding is performed in less than 2 minutes, the grinding is not sufficient, so it is difficult to evenly disperse in distilled water, and when it exceeds 3 minutes, there is no further effect, so it is uneconomical.

At this time, the tissue grinder 10 is provided with a glass tube 16 and a body 11 that is inserted into the glass tube and operated, and the lower end of the body 11 has a crushing rod ( 12) is provided, and a handle 14 and a handle 15 are provided at the upper end of the body 11 so that the liquid seed medium composition and distilled water are put into the glass tube 16, and the tissue is crushed while rotating the grinding rod 12 (see FIGS. 3 and 4).

The space between the outer circumferential surface of the grinding rod 12 and the inner circumferential surface of the glass tube 16 is 0.010 to 0.015 mm, thereby enabling the tissue to be easily crushed.

After the liquid seed medium composition is prepared, it can be cultured by inoculating medicinal mushroom seed. However, in the case of inoculating the existing medicinal mushroom spawn as it is, substrate competition may occur between each mushroom.

To this end, the medicinal mushroom seed is prepared from a pulverized tree comprising at least one selected from the group consisting of pine, fir, oak, chestnut and acacia; Sterilizing the pulverized wood at a temperature of 100 to 150° C. for 50 to 100 minutes; A water supply step of making the water content of the sterilized pulverized wood to be 30 to 40% by weight; inoculating the mycelium of Sanghwang mushroom, chaga mushroom, horseshoe mushroom and reishi mushroom into the water-supplied pulverized water; Culturing the mycelium of the situation mushroom, chaga mushroom and reishi mushroom at a temperature of 20-25° C. for 20-40 days after inoculation is completed; obtaining a fruiting body after the culturing is completed; mixing 5 to 10 parts by weight of Cordyceps Cordyceps relative to 100 parts by weight of the obtained fruiting body; and drying the obtained mixture of fruiting bodies and Cordyceps cordyceps, and preparing medicinal mushroom spawns.

The fruiting body of the present invention refers to a mushroom spore that grows to the upper part of the medium when the mushroom is cultured, and generally refers to a part of a mushroom used for food. In the present invention, in order to cultivate such a fruiting body, it is possible to use a pulverized tree containing at least one selected from the group of pine, fir, oak, chestnut, and acacia. In general, in the case of mushrooms, sawdust is used as the medium, and even in the case of the present invention, the pulverized product of the tree, that is, sawdust can be used for culturing the fruiting body.

The pulverized wood may be subjected to a sterilization and moisture supply step before inoculation of the seed of the mushroom.

The sterilization step is performed to remove microorganisms and other types of mycelium contained in the sawdust. Unsuccessful fruiting bodies may prosper and culture may fail. Therefore, it is preferable to enable the cultivation of the desired fruiting body through the sterilization process as described above. In addition, since the sterilization is performed at a high temperature, it can be concomitantly weakened the tissue of the sawdust to facilitate culturing of the mushroom fruiting body. In this case, the sterilization is preferably performed at a temperature of 100 to 150° C. for 50 to 100 minutes. If the temperature of the sterilization is less than 100 ℃ or if it is performed for less than 50 minutes, sterilization is not complete, so it may be difficult to cultivate the desired fruiting body. Economical.

The sterilized pulverized wood as described above may be supplied with moisture so that the moisture content is 30 to 40% by weight for the cultivation of medicinal mushrooms. Since the cultivation of medicinal mushrooms, which will be described later, requires an appropriate amount of moisture, it is preferable to supply moisture so as to have a moisture content of 30 to 40% by weight as described above.

After drying the water content of the pulverized wood to 30 to 40% by weight, the mycelium of Shanghai mushroom, chaga mushroom, horseshoe mushroom and reishi mushroom can be inoculated. In the case of the situation mushroom, chaga mushroom, horseshoe mushroom, and reishi mushroom, the fruiting body corresponds to a species close to wood, and thus requires less moisture than the conventional mushroom culture. Therefore, the moisture content of the pulverized wood is adjusted to 30 to 40% by weight, and the seeds of the Sangha mushroom, chaga mushroom, horseshoe mushroom and reishi mushroom are inoculated and cultured. In addition, since the multi-species mushrooms are mixed in this process to form mycelium and fruiting body, even when used as a liquid medium to be thereafter, the mycelium of each mushroom can be easily mixed and grown.

The mycelium of the Sangha mushroom, chaga mushroom, horseshoe mushroom and reishi mushroom can be cultured for 20-40 days at a temperature of 20-25 ℃ after the inoculation is completed. In the case of the above culture, it is preferable to incubate for 20 to 40 days at a temperature of 20 to 25 °C. At this time, if the temperature is less than 20 ℃ or if cultured for a period of less than 20 days, the mycelium of the situation mushroom, chaga mushroom, horseshoe mushroom and reishi mushroom may not completely spread to the wood pulverized material, and the temperature exceeding 25 ℃ or In the case of culturing for more than 40 days, culturing may fail for cereals, which will be described later, due to overcultivation.

After the culture is completed as described above, fruiting bodies can be obtained, and 5 to 10 parts by weight of Cordyceps Cordyceps relative to 100 parts by weight of the obtained fruiting body can be mixed and used. In the case of Cordyceps, it is possible to form a large amount of beta-glucan, but since it has a different ecology from the mushrooms, it is impossible to mix and grow them. Therefore, in the case of the Cordyceps cordyceps, it is preferable to use the mycelium of the Cordyceps cordyceps by collecting the fruiting bodies of the cultured mushrooms and then mixing them.

At this time, in the case of the Cordyceps Cordyceps, 5 to 10 parts by weight may be mixed with respect to 100 parts by weight of the fruiting body of the collected mushroom. When the amount of Cordyceps Cordyceps is mixed in less than 5 parts by weight, it is difficult to expect the effect of the Cordyceps cordyceps, and when it is mixed in excess of 10 parts by weight, the ratio of Cordyceps cordyceps increases, so it may be difficult to culture the mycelium of other mushrooms.

A mushroom medium can be prepared by drying the obtained mixture of fruiting bodies and Cordyceps. In the case of a basic mushroom medium, the growth rate is different depending on the type of each mushroom and at the same time substrate competition occurs between the mushrooms when culturing the mycelium in the mushroom medium as it is prepared by simply grinding and mixing the collected fruiting bodies. Although it can be seen, in the present invention, since the fruiting body is grown by mixing and growing mushrooms in the mycelium stage through the method as described above, each mushroom can grow to form a kind of mushroom group. Therefore, in the case of the mushroom medium used in the present invention, it can show a high growth rate compared to the existing mushroom medium.

The liquid seed medium composition may be inoculated with medicinal mushroom seed and cultured for 1 to 30 hours. As the medicinal mushroom seed, a pulverized product of the obtained fruiting body may be used, inoculated into the liquid seed medium composition, and then cultured for 1 to 30 hours to be used as a medicinal mushroom liquid seed medium composition. At this time, if the incubation time is less than 1 hour, the culture of the mushroom spawn in the medicinal mushroom liquid starter medium composition is not made, so it may be difficult to culture the mycelium in cereals. can

In addition, in the culture process, the liquid seed medium composition inoculated with the medicinal mushroom seed can be cultured while homogenizing using a magnetic bar and a magnetic stiller. In the culturing process, it is preferable that the liquid medium composition is uniformly dispersed in the seed cell, and the mycelium produced by the seed cell is pulverized and homogenized. Therefore, it is preferable to homogenize the liquid medium composition by continuously mixing the liquid medium composition using a magnetic bar and a magnetic stiller.

The liquid medicinal mushroom seed medium composition prepared as described above may be inoculated into the cooled grains to form mycelium on the surface of the grains. At this time, the existing inoculation method is limited to simply mixing and culturing the seed with grains, making it difficult to uniformly mix and separate the seed after culturing to commercialize it. In order to improve this, a method using liquid spawn is used, but it is limited to simply injecting from the top of the grain, so there is a limit to uniform culture. In the present invention, since the liquid seed culture medium composition for medicinal mushrooms is uniformly injected using a needle-type nozzle, the mycelium can be uniformly formed in the grains, and even after the culture is completed, it can be commercialized without any separation process.

To this end, the step of inoculating the liquid medicinal mushroom seed culture medium composition into the grains in the container may include: loading the medicinal mushroom liquid seed culture medium composition into an injector; penetrating the needle-shaped nozzle formed on one side of the injector through the film constituting the wall surface of the container so that the nozzle is located inside the container; spraying the medicinal mushroom liquid seed medium composition through a needle-type nozzle inside the container by pressing the injector; and after removing the nozzle, closing the through hole formed by the nozzle.

The injector is used to inject the medicinal mushroom liquid spawning medium composition into the grains in the container and includes a needle-type nozzle on one side. It can be sprayed through. In addition, in the case of the needle-type nozzle, it is preferable that 1 to 5 injection holes are formed in order to spray the liquid medicinal mushroom seed culture medium composition in all directions within the container.

Looking at this in detail, after the medicinal mushroom liquid spawning medium composition is charged into the injector, a needle-shaped nozzle installed on one side of the injector is inserted through the wall of the container into the container. Since part or all of the wall surface of the container is made of a film, the needle-shaped nozzle can be easily penetrated and inserted.

As described above, when the needle-shaped nozzle is inserted and then the injector is pressurized, the medicinal mushroom liquid spawning medium composition can be sprayed through the injection hole of the needle-shaped nozzle while being pressurized, through which it can be uniformly sprayed on the grains. can However, in the case of the grain, the medicinal mushroom liquid spawning medium composition is sprayed only to a part of the grain through the spraying as described above, so spraying using the needle-shaped nozzle is repeated 2 to 4 times, but at each spraying, the needle-type nozzle is When the insertion is performed at different positions, the medicinal mushroom liquid seed medium composition may be sprayed on the whole grain.

In addition, after the injection is completed as described above, the through-hole formed by the furnace can be closed in order to re-seal the container. can

After the inoculation is completed, medicinal mushroom mycelium can be cultured on the surface of the grain. In the culturing step of the medicinal mushroom mycelium, light of 700 to 1000 lux can be supplied. The light can increase the color development of the mushroom mycelium and at the same time increase the content of polyphenol used for the color development, thereby improving the effect of the medicinal mushroom mycelium. When the light irradiation is 700 lux or less, the desired color cannot be obtained in cereals, and when it is 1000 lux or more, the content of the active ingredient including cordycepin is reduced, so the light irradiation is preferably 700 to 1000 lux.

The step of culturing the medicinal mushroom mycelium on the surface of the grain may be performed at a temperature of 15 to 30° C. for 30 to 60 days. If the temperature is less than 15 ℃ or if the culture is performed for a period of less than 30 days, it is difficult to expect the effect of the medicinal mushroom mycelium because the culture of the mycelium is not sufficient, and the temperature exceeds 30 ℃ or a discipline exceeding 60 days If cultured during the period, fruiting bodies may be formed or off-flavor may occur due to overcultivation. In particular, when a fruiting body is formed, active ingredients and nutrients contained in the mycelium can move to the fruiting body, and a fruiting body such as downy can be formed.

After the cultivation of the medicinal mushroom mycelium is completed as described above, it is possible to release the agglomerated portion of the grain. In the case of the medicinal mushroom mycelium, since it has a characteristic of growing by bonding with an adjacent mycelium, when grown on the surface of the grain, the grain may agglomerate and agglomerate. In the case of agglomerated grains, not only may the commercial properties deteriorate, but also mycelium may overgrow therein. Therefore, in the case of grains that are bunched as described above, it is preferable to separate each grain by loosening the clumped portion after the culturing of the medicinal mushroom mycelium is completed. This process may be performed by simply rubbing the grains by hand, or it is also possible to separate grains by using a stirrer or simply by applying periodic vibrations to the container. In addition, the separation of grains as described above is preferably performed after the culturing of the medicinal mushroom mycelium is completed, but may be performed 1 to 5 times while culturing the medicinal mushroom mycelium if necessary.

After the culture is completed as described above, the grains are dried with hot air to evaporate excess moisture and prevent further growth of mycelium (FIG. 6).

Hereinafter, preferred embodiments of the present invention will be described so that those of ordinary skill in the art can easily implement them with reference to the accompanying drawings. In addition, in the description of the present invention, if it is determined that a detailed description of a related known function or a known configuration may unnecessarily obscure the gist of the present invention, the detailed description thereof will be omitted. In addition, certain features presented in the drawings are enlarged, reduced, or simplified for ease of description, and the drawings and components thereof are not necessarily drawn to scale. However, those skilled in the art will readily appreciate these details.

Example 1

After washing brown rice produced in Nonsan, North Chungcheong Province, immersed in water for 12 hours, and placed on a sieve to remove excess moisture to prepare grains.

500 g of the brown rice was loaded into a plastic bag made of vinyl, and then sealed using a stopper having a filter.

It was charged into the sealed container autoclave and sterilized by heating at 105° C. for 35 minutes and at 121° C. for 35 minutes using water vapor supplied at a pressure of 15 psi.

After the sterilization was completed, the container was taken out and cooled to a temperature of 25° C. and prepared.

The pulverized products of pine, fir, oak, and chestnut were mixed in equal amounts, and then sterilized at a temperature of 120° C. for 80 minutes. Water was supplied to the sterilized pulverized wood to adjust the moisture content to 35% by weight, and after the moisture was supplied, the mycelium of Shanghai mushroom, chaga mushroom, horseshoe mushroom and reishi mushroom was inoculated, and the temperature was 22° C. for 30 days. cultured.

After the culture was completed, the fruiting body that grew on the top of the sawdust pulverized product was recovered, and then 5 parts by weight of Cordyceps Cordyceps relative to 100 parts by weight of the fruiting body was mixed and used as a mushroom spawn.

The mushroom spawn was sterilized at a temperature of 100° C. for 20 minutes, dried and pulverized after sterilization was completed.

Mixed in a ratio of 0.68g peptone Powder, 0.56g malt extract Powder, 0.56g yeast extract Powder, 7.5g dextrose, 1g soybean Powder and put into a tissue grinder, grinded for 2 minutes, and the grinded mixture was added to 1L of water to prepare a liquid medium. To 100 parts by weight of the liquid medium, 1 g of the pulverized mushroom spawn was added, and the liquid seed medium composition was homogenized using a magnetic bar and a magnetic stiller, and cultured for 20 hours to prepare a liquid seed medium composition inoculated with medicinal mushroom spawn.

Using an injector having a needle-type nozzle (three injection ports) in the container, 10 ml of the liquid seed medium composition inoculated with the medicinal mushroom spawn was sprayed at different locations three times, and after the spraying was completed, the through hole was closed using a hot melt. did.

The container in which the liquid seed medium composition inoculated with the medicinal mushroom seed was injected was cultured for 40 days at a temperature of 25 ° C. After the culture was completed, the brown rice was taken out from the container, the grains were released and dried with hot air at 150 ° C. for 5 minutes.

Example 2

In Example 1, the same procedure was performed except that the mycelium of Sanghwang mushroom, chaga mushroom, horseshoe mushroom, reishi mushroom and cordyceps mycelium was purchased and then crushed and used without mixing culture in Example 1.

Example 3

The same procedure was performed except that in Example 1, the mushroom spawn was directly mixed with grains without using a liquid medium and cultured.

Example 4

In Example 1, the same procedure was performed except that 30 ml of the liquid seed medium composition inoculated with the medicinal mushroom spawn from the upper part was added at once without using an injector.

Experimental Example 1

Salsi In the brown rice containing the mycelium of mushrooms prepared in Examples 1 to 4, it was separated into brown rice in which the mycelium was formed and brown rice in which the mycelium was not formed, and then the ratio was confirmed. In the case of brown rice in which mycelium was formed, it was confirmed that the mycelium was observed on the surface or the color was changed (see FIG. 6 ).

Example 1 Example 2 Example 3 Example 4 ratio 94.8% 81.6% 38.4% 59.5%

As shown in Table 1, in the case of Example 1 of the present invention, it was confirmed that the mycelium was formed at a high ratio. However, in the case of Example 2 in which the mixed cultured fruiting bodies were not used, substrate competition occurred between each mushroom, and it was confirmed that the mycelium formation rate was reduced, and in Example 3 without using a liquid medium, the mycelium formation was It was found to show the lowest formation rate because it was not easy.

Also, in the case of Example 4, in which the liquid medium was supplied from the upper part as in the conventional method, it was confirmed that it took a lot of time for the mycelium to reach the lower part. That is, in the case of Example 1 of the present invention, a uniform mycelium can be cultured even for a short period of time, but in the case of other examples, it was confirmed that a longer culture period was required to culture the mycelium at the same level as the present invention.

Experimental Example 2

Brown rice containing the mycelium of mushrooms prepared by the method of Example 1 above was sent to the Food Analysis Research Center of Suwon Women's University with application number A5-191114-106-01, A5-191114-106-02, A5-191114-106- 03 and A5-190509-003-01, request component analysis was performed.

As shown in FIG. 7 , it was confirmed that aflatoxin, a representative toxic substance of mushrooms, was not detected, and the content of lead, cadnium, arsenic and mercury was found to be less than the standard value ( FIG. 8 ). In addition, as shown in FIG. 9, E. coli cells were confirmed to be negative, indicating that they can be used for food. In addition, in the pesticide residue test of FIG. 10, it was confirmed that the remaining components except for those detected in Abamectin et al. 319 were non-detectable.

In the above, preferred embodiments of the present invention have been illustrated and described, but the present invention is not limited to the specific embodiments described above, and it is common in the technical field to which the present invention pertains without departing from the gist of the present invention as claimed in the claims. Various modifications are possible by those having the knowledge of, of course, these modifications should not be individually understood from the technical spirit or perspective of the present invention.

10: tissue grinder
11: body
12: grinding rod
13: uneven part
14: handle
15: handle
16: glass tube

Claims (7)

Washing the grains from which foreign substances have been filtered, and immersing them in water;
After the immersion is completed, placing the grains on a tray and removing the water;
loading the grains from which the water has been removed into a container and then closing a stopper having a filter;
high-pressure sterilization of the grains in the container;
Naturally evacuating the sterilized container and cooling it at the same time;
inoculating the medicinal mushroom liquid seed medium composition into the grains in the container after the cooling is completed;
culturing medicinal mushroom mycelium on the surface of cereals after the inoculation is completed; and
After the culturing is completed, loosening the lumps of grains and drying with hot air;
In a grain manufacturing method comprising the mycelium of mushrooms using a liquid medium comprising:
The container is a container in which part or all of the wall surface is made of a film,
Inoculating the medicinal mushroom liquid seed medium composition to the grains in the container comprises:
loading the medicinal mushroom liquid seed medium composition into an injector;
penetrating the needle-shaped nozzle formed on one side of the injector through the film constituting the wall surface of the container so that the nozzle is located inside the container;
spraying the medicinal mushroom liquid seed medium composition through a needle-type nozzle inside the container by pressing the injector; and
removing the nozzle and then closing the through hole formed by the nozzle;
includes,
The needle-type nozzle is formed with 1 to 5 injection holes,
The medicinal mushroom liquid seed culture medium composition is a method for producing grains containing the mycelium of mushrooms using a liquid medium, characterized in that the container is inoculated 2 to 4 times.
According to claim 1,
The step of high-pressure sterilization (sterilization) treatment,
a primary sterilization step of heating the grains in the container at 101 to 105° C. for 30 to 40 minutes; and
a second sterilization step of heating the grains at 120 to 125° C. for 30 to 40 minutes after the first sterilization step is completed;
Grain manufacturing method comprising the mycelium of mushrooms using a liquid medium, characterized in that it comprises a.
According to claim 1,
The medicinal mushroom liquid seed culture medium composition,
0.05 to 0.07 parts by weight of peptone powder relative to 100 parts by weight of water, 0.04 to 0.06 parts by weight of malt extract powder, 0.05 to 0.06 parts by weight of yeast extract Powder, dextrose ) A method for producing grains containing mycelium of mushrooms using a liquid medium, characterized in that it is a mixture of 0.5 to 0.8 parts by weight and 0.05 to 0.2 parts by weight of soybean Powder.
4. The method of claim 3,
The medicinal mushroom liquid seed culture medium composition,
Put a mixture of peptone Powder, malt extract Powder, yeast extract Powder, dextrose and soybean Powder in a tissue grinder, and for 2-3 minutes grinding;
mixing the pulverized mixture with distilled water to prepare a liquid seed medium composition; and
inoculating the medicinal mushroom seed into the liquid seed medium composition and culturing for 1 to 30 hours;
It is prepared by a method comprising
The medicinal mushroom includes at least one selected from the group of Cordyceps Cordyceps, Shanghai Mushroom, Reishi Mushroom, Horseshoe Mushroom and Chaga Mushroom,
The culturing step is a method for producing a grain comprising a mycelium of mushrooms using a liquid medium, characterized in that the culturing is a step of homogenizing the liquid seed medium composition inoculated with medicinal mushroom spawn using a magnetic bar and a magnetic stiller and culturing.
5. The method of claim 4,
The medicinal mushroom spawn,
Preparing a pulverized wood comprising at least one selected from the group of pine, fir, oak, chestnut and acacia;
Sterilizing the pulverized wood at a temperature of 100 to 150° C. for 50 to 100 minutes;
A water supply step of making the water content of the sterilized pulverized wood to be 30 to 40% by weight;
inoculating the mycelium of Sanghwang mushroom, chaga mushroom, horseshoe mushroom and reishi mushroom into the water-supplied pulverized water;
Culturing the mycelium of the situation mushroom, chaga mushroom and reishi mushroom for 20-40 days at a temperature of 20-25 ℃ after the inoculation is completed;
obtaining a fruiting body after the culturing is completed;
mixing 5 to 10 parts by weight of Cordyceps Cordyceps relative to 100 parts by weight of the obtained fruiting body; and
drying the obtained mixture of fruiting bodies and Cordyceps Cordyceps to prepare a medicinal mushroom seed;
Grain manufacturing method comprising the mycelium of mushrooms using a liquid medium, characterized in that it is prepared by a method comprising a.
delete A cereal comprising the mycelium of mushrooms produced by the method of any one of claims 1 to 5.

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