KR102308743B1 - Cosmetic composition comprising complex for alleviating skin irritation with lamella liquid crystal structure - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a skin irritation alleviation complex having excellent skin barrier recovery and moisturizing effects, and more particularly, to a skin irritation relief complex containing madecassoside, allantoin and panthenol in a high content, It relates to a cosmetic composition for alleviating skin irritation of the lamellar liquid crystal type that increases the penetration rate and enhances the stability of the complex.

Description

Lamella liquid crystal cosmetic composition comprising a skin irritation alleviation complex {COSMETIC COMPOSITION COMPRISING COMPLEX FOR ALLEVIATING SKIN IRRITATION WITH LAMELLA LIQUID CRYSTAL STRUCTURE}

The present invention relates to a cosmetic composition comprising a skin irritation alleviation complex having excellent skin barrier recovery and moisturizing effects, and more particularly, includes a skin irritation alleviation complex in a high content, increases skin penetration, and improves the stability of the complex Shiki relates to a lamellar liquid crystal type cosmetic composition for alleviating skin irritation.

The skin is the largest organ in the human body and is also the first defense network against the external environment. However, the skin is the outermost layer that is directly exposed to the external environment, and the activity of keratinocytes and fibroblasts decreases due to ultraviolet rays, stress, fine dust, and harmful chemicals. It becomes sensitive to various stimuli due to a decrease in the ability to protect against stimuli. The skin sensitized by external stimuli may exhibit objective irritants such as erythema and edema, and subjective inflammatory reactions such as itching, stinging, and burning. irritation may occur.

Cosmetics are used to clean the body, make it beautiful, protect the skin and keep it healthy. However, looking at the cosmetic composition, ingredients different from the original skin protection purpose are used as essential for product manufacturing and maintenance. These ingredients are indispensable for the preservation, maintenance and function of cosmetics, and include surfactants, preservatives, fragrances, and sunscreens. These ingredients are generally known as the main cause of irritation, inflammation, allergies, pimples, edema, etc. on the skin.

Therefore, in terms of skin safety, cosmetics that reduce skin irritation and inflammation caused by various irritants are required, and the importance of irritation-relieving materials that can protect the skin from various factors is increasing.

The skin consists of three layers: the epidermis, the dermis, and the subcutaneous fat and surrounds the whole body. Among them, the epidermis consists of the basal layer, the stratum corneum, the granular layer and the stratum corneum. The stratum corneum, the outermost layer of the skin, plays a role of a strong skin barrier that protects the skin from external stimuli, so it is difficult to penetrate drugs. Therefore, various delivery systems such as liposomes, nanoparticles, hydrogels, and liquid crystal emulsions have been developed as a way to overcome these skin barriers and enhance drug permeation efficiency.

Among them, the liquid crystal emulsion forms a structure similar to the lamellar structure of the skin, so that it is effectively fused to the skin intercellular lipids and has a multi-layered lamellar structure, so that the inclusion material can be more stably maintained. Unlike general O/W formulations, liquid crystal emulsions are effective cosmetic formulations for formulation stability, skin absorption of effective substances, and transepidermal water loss (TEWL). Therefore, it is expected that the liquid crystal emulsion can help alleviate or improve various skin irritations.

Korean Patent Registration No. 10-0965088 discloses a multi-layered lamellar vesicle that is composed of a phospholipid formulation containing phospholipids to stabilize the active ingredient.

In addition, Korean Patent No. 10-1466650 discloses a hypoallergenic and highly moisturizing oil-in-water cosmetic composition comprising a polyol and an emulsifier.

The present invention obtains a new skin irritation relief complex by combining materials with various different irritation alleviation mechanisms for substances that have already been proven safe in cosmetics, and increases skin absorption by preparing a lamellar liquid crystal cosmetic composition comprising the same In addition, an object of the present invention is to provide a cosmetic composition that increases stability for a long time and can have an excellent skin barrier recovery and irritation alleviation effect.

The above object is achieved by a lamellar liquid crystal type cosmetic composition for alleviating skin irritation containing a skin irritation alleviation complex including madecassoside, allantoin and panthenol.

Preferably, the skin irritation alleviation complex may include madecassoside, allantoin and panthenol in a weight ratio of 1 to 2: 4 to 5: 4 to 5.

Also preferably, the skin irritation alleviation complex may be included in an amount of 0.1 to 10.0% by weight based on the total weight of the cosmetic composition.

Also preferably, the lamellar liquid crystal may be formed by including an oil phase containing a nonionic surfactant, a higher alcohol and a lipid component, and an aqueous phase containing water, a polyol, and a skin irritation alleviation complex.

Also preferably, the nonionic surfactant is at least one selected from the group consisting of C12-20 alkyl glucoside, potassium cetyl phosphate and sorbitan palmitate, and the lipid component is ceramide-3, cholesterol, lecithin and caprylic. / at least one selected from the group consisting of capric triglyceride, and the higher alcohol may be C14-22 alcohol and behenyl alcohol.

Also preferably, the lamellar liquid crystal contains 1 to 30% by weight of a nonionic surfactant, 1 to 10% by weight of a higher alcohol, 0.1 to 10% by weight of a lipid component, 20 to 30% by weight of a polyol relative to the total weight of the cosmetic composition, skin irritation relief It may contain 0.1 to 10.0 weight % of the complex and the balance of water.

The skin irritation alleviation complex according to the present invention can provide a lamellar liquid crystal cosmetic composition having excellent skin barrier recovery and irritation alleviation effects, as well as being encapsulated in lamellar liquid crystals and having high skin absorption and stability.

1 is a polarization micrograph (400 times) of a lamellar liquid crystal (Example 1) and an O/W emulsion (Comparative Example 1) according to the present invention.
2 is a Cyro-SEM photograph of a lamellar liquid crystal (Example 1) according to the present invention.
3 is a result of measuring the skin permeability of the lamellar liquid crystal cosmetic composition and the O/W emulsion cosmetic composition according to the present invention.
4 is a result of measuring the improvement rate of the amount of transdermal moisture loss of the lamellar liquid crystal cosmetic composition and the O/W emulsion cosmetic composition according to the present invention.
5 is a result of measuring the erythema improvement rate of the lamellar liquid crystal cosmetic composition and the O/W emulsion cosmetic composition according to the present invention.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and have the meanings as commonly understood by one of ordinary skill in the art in the relevant field of the present invention. In addition, although preferred methods and samples are described herein, similar or equivalent ones are also included in the scope of the present invention.

The term "about" refers to 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length varying by 4, 3, 2 or 1%.

Throughout this specification, unless the context requires otherwise, the terms "comprises" and "comprising" include the steps or elements presented, or groups of steps or elements, but any other step or element, or It is to be understood that a step or group of elements is not excluded.

The present invention relates to a lamellar liquid crystal type cosmetic composition for alleviating skin irritation containing madecassoside, allantoin and panthenol as active ingredients.

The cosmetic composition for alleviating skin irritation of the present invention is characterized in that it forms a lamellar liquid crystal, including an oil phase containing a nonionic surfactant, a higher alcohol and a lipid component, and an aqueous phase containing water, a polyol, and an irritation alleviation complex.

According to one embodiment of the present invention, the madecassoside, allantoin and panthenol are mixed in a weight ratio of 1 to 2: 4 to 5: 4 to 5 to form a skin irritation alleviation complex.

Preferably, the skin irritation alleviation complex may be included in the lamellar liquid crystal. The skin irritation alleviation complex is included in the lamellar liquid crystal, and may be contained in an amount of 0.1 to 10.0% by weight based on the total weight of the cosmetic composition.

According to an embodiment of the present invention, the lamellar liquid crystal includes an oil phase and an aqueous phase.

The oil phase includes a nonionic surfactant, a higher alcohol and a lipid component, and the aqueous phase includes water, a polyol, and a skin irritation alleviation complex.

The nonionic surfactant is at least one selected from the group consisting of C12-20 alkylglucoside, potassium cetyl phosphate and sorbitan palmitate. The nonionic surfactant may be included in an amount of 1 to 30% by weight based on the total amount of the cosmetic composition.

The lipid component may be at least one selected from the group consisting of ceramide-3, cholesterol, lecithin and caprylic/capric triglyceride. The lipid component may be included in an amount of 0.1 to 10% by weight relative to the total amount of the cosmetic composition.

The higher alcohol may be C14-22 alcohol and behenyl alcohol. The higher alcohol may be included in an amount of 1 to 10% by weight based on the total amount of the cosmetic composition.

The polyol may be at least one selected from the group consisting of glycerin, polyethylene glycol, propylene glycol, 1,3-butylene glycol, propanediol, and ethyl hexanediol. The polyol may contain 20 to 30% by weight of the total amount of the cosmetic composition, 0.1 to 10.0% by weight of the skin irritation alleviation complex, and the remaining amount of water.

According to one embodiment of the present invention, the particles of the lamellar liquid crystal structure may further include a physiologically active material that helps to relieve skin irritation. The physiologically active substance may include dipotassium glycyrrhizate, beta-glucan, mackerel extract and comfrey extract.

According to one embodiment of the present invention, the lamellar liquid crystal cosmetic composition is prepared by the following method.

First, a multi-layered oil phase is prepared by mixing the oil phase components ceramide, hydrogenated lecithin, cholesterol, caprylic/capric triglyceride (abnormal lipid component), C12-20 alkyl glucoside, C14-22 alcohol, and behenyl alcohol. Next, purified water, polyol, and skin irritation alleviation complex (madecassoside, allantoin and panthenol) are added to prepare an aqueous phase. After dissolving the oil phase part and the aqueous phase part at 70-90°C, preferably at 80°C, respectively, the first emulsification is performed using a homomixer. Then, secondary emulsification is performed using a high-pressure type emulsifier to make a lamellar liquid crystal structure.

Hereinafter, the present invention will be described in more detail through non-limiting examples. However, the following examples are intended to illustrate the present invention, and the scope of the present invention is not to be construed as being limited by the following examples.

manufacturing example 1: Preparation of skin irritation alleviation complex

The skin irritation alleviation complex of the present invention is a mixture of madecassoside, allantoin, and panthenol showing different stimulation relief mechanisms, thereby exhibiting a synergistic irritation relief effect. The skin irritation alleviation complex was used by mixing in a weight ratio of madecassoside: allantoin: panthenol = 10 wt%: 45 wt%: 45 wt%. This was compared with Formulation Example 1 containing only 0.1% by weight of madecassoside, Formulation Example 2 containing only 0.5% by weight of allantoin, and Formulation Example 3 containing only 0.5% by weight of panthenol as shown in Table 1 below.

Experimental example 1: Primary irritation evaluation of skin irritation relief complex (patch test)

When the skin irritation alleviation complex of the present invention is directly applied to human skin, a human skin patch test was performed to confirm whether it has an excellent skin irritation alleviation effect. The test subject visited a total of three times (before the start of the test, on the date of removing the patch, and after 30 minutes after removing the patch) during this human application test period to determine whether or not the skin was irritating. At the first visit, a skin patch test was performed on the subject's back using a Finn chamber (100×10, EPITEST, Finland). For 15 test subjects, the sample was applied between the shoulder blade and the waistline with the spine on the back using a pin chamber.

After wiping the test site with 70% (v/v) ethanol and drying, 20 μl of the test sample prepared according to Table 1 below was dropped into the pin chamber and fixed on the test site. As a test sample, 0.1% madecassoside in Formulation Example 1, 0.5% allantoin in Formulation Example 2, 0.5% panthenol in Formulation Example 3 was added, and in Formulation Example 4 madecassoside: allantoin: panthenol = 10 wt%: 45 wt% %: The mixture was added at a weight ratio of 45% by weight, and Comparative Formulation Example 1 without the addition of an irritant reliever was tested together. They were applied to the skin for 24 hours. After the patch was removed, the test site was marked with a marking pen, and after 30 minutes, erythema and edema of the test site were observed using a magnifying glass (8MC-150, DAZOR, USA).

Raw material name content( weight% ) Formulation example One Formulation example 2 Formulation example 3 Formulation example 4 Comparative formulation example One Madecassoside 0.1 - - - - allantoin - 0.5 - - - panthenol - - 0.5 - - Alleviation complex - - - 0.5 - lactic acid 5 5 5 5 5 glycerin 2.8 2.8 2.8 2.8 2.8 Polyglyceryl-3 methylglucose distearate 1.6 1.6 1.6 1.6 1.6 PEG-4 Oliveate 0.4 0.4 0.4 0.4 0.4 cetearyl alcohol 0.2 0.2 0.2 0.2 0.2 squalane 4.5 4.5 4.5 4.5 4.5 Caprylic/Capric Triglycerides 4.2 4.2 4.2 4.2 4.2 dimethicone 1.3 1.3 1.3 1.3 1.3 Hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer 1.0 1.0 1.0 1.0 1.0 incense a very small amount a very small amount a very small amount a very small amount a very small amount Distilled water remaining amount remaining amount remaining amount remaining amount remaining amount

The skin reaction was determined according to the regulations (Table 2) of the International Contact Dermatitis Research Group (ICDRG), and the average skin reactivity was calculated according to Equation 1 below, and the results are shown in Table 3.

- no response 0 . faint light erythema 0.5 ± Mild erythema with well-demarcated borders One + Distinct erythema, papules and vesicles 2 ++ Severe erythema, blistering 6 +++ crust formation 12

The degree of skin irritation in Experimental Example 1 was shown according to the evaluation criteria as shown in Table 2, and the skin irritation rate was calculated by the following calculation formula and shown in Table 3.

The degree of skin irritation is judged as non-irritating if the stimulation rate is 0.0, non-irritating if 0.1-1.0, mild if 1.0-1.7, moderately irritating if 1.7-2.5, and strong if over 2.5.

composition skin irritation rate stimulus degree Formulation Example 1 1.67 mild stimulation Formulation Example 2 1.39 mild stimulation Formulation Example 3 1.53 mild stimulation Formulation Example 4 1.11 mild stimulation Comparative Formulation Example 1 2.08 central stimulus

composition Stimulus relief rate (%) Comparative Formulation Example 1 - Formulation Example 1 19.71 Formulation Example 2 33.17 Formulation Example 3 26.44 Formulation Example 4 46.63

As can be seen from Experimental Example 1 of the present invention, it was confirmed that the composition formulation containing 5.0% (w/w) lactic acid caused strong irritation to the skin. It was confirmed that Formulation Example 4, which is a cosmetic containing the alleviation complex, had a significantly lower skin irritation rate than Comparative Formulation Example 1, which did not contain the skin irritation relief complex. In particular, compared with the skin irritation rate of Formulation Examples 1 to 3, it was found that the irritation relief effect was increased when each anti-irritant agent was contained together as in the irritation relief complex of the present invention.

And, as can be seen from Tables 3 and 4, it was confirmed that the material in which madecassoside, allantoin, and panthenol of the present invention were mixed together in a certain ratio increased the irritation-relieving effect further against the stimulation of lactic acid.

manufacturing example 2: lamella liquid crystal emulsion composition preparation

A lamellar liquid crystal emulsion of Example 1 was prepared according to the method presented below with the composition of Table 5 below. First, a multi-layered oil phase was prepared by mixing ceramide, hydrogenated lecithin, cholesterol, C12-20 alkyl glucoside, C14-22 alcohol, caprylic/capric triglyceride, potassium cetyl phosphate, and behenyl alcohol. Next, an aqueous phase was prepared by adding a skin irritation alleviation complex together with purified water and glycerin. At this time, the skin irritation alleviation complex was used in a weight ratio of madecassoside: allantoin: panthenol = 10% by weight: 45% by weight: 45% by weight. Then, the oil phase and the aqueous phase were respectively dissolved at 80° C., and then the primary emulsification was performed using a homomixer as follows. After the oil phase was added to the dispersed aqueous phase, emulsification was stirred at 80° C. and 1500 rpm for 5 min. The prepared sample was subjected to secondary emulsification using a high-pressure emulsifier. After high pressure emulsification once while maintaining 80 ℃, it was cooled while slowly stirring to 30 ℃.

The O/W emulsion of Comparative Example 1 was prepared by mixing ceramide, hydrogenated lecithin, cholesterol, glyceryl stearate, PEG-100 stearate, caprylic/capric triglyceride, potassium cetyl phosphate, and behenyl alcohol to form an oil phase. The aqueous phase was prepared by adding a skin irritation alleviation complex with purified water and glycerin. Then, the oil phase and the aqueous phase were respectively dissolved at 80° C., and then the primary emulsification was performed using a homomixer as follows. After the oil phase was added to the dispersed aqueous phase, emulsification was stirred at 80 °C and 1500 rpm for 5 min. The prepared sample was cooled to 30 °C with slow stirring.

Raw material name ( weight% ) Example One Comparative Example One note glyceryl stearate - 2.00 paid PEG-100 Stearate - 2.00 C12-20 Alkyl Glucoside 0.80 - C14-22 alcohol 3.20 - Potassium Cetyl Phosphate 1.00 1.00 Ceramide-3 0.01 0.01 cholesterol 0.10 0.10 hydrogenated lecithin 0.20 0.20 Caprylic/Capric Triglycerides 25.00 25.00 behenyl alcohol 6.00 6.00 glycerin 25.00 25.00 Awards Distilled water 35.97 35.97 Alleviation complex 2.00 2.00 metal ion sequestrant 0.02 0.02 Napri Natural Preservative 0.70 0.70 additive Sum 100.00 100.00

Experimental example 2: Particle observation

(1) Polarized microscope observation

A nonionic surfactant and higher alcohol were used to prepare the lamellar liquid crystal emulsion, and the content was 0.8 wt% of C12-20 alkylglucoside, 3.2 wt% of C14-22 alcohol, and 6 wt% of behenyl alcohol. 1 shows a photograph observed under a polarization microscope at 400 magnification. In a polarization microscope, a maltese cross pattern is observed because of the phase difference in which the speed of light passing through the liquid crystal emulsion with optical anisotropy is different depending on the major axis and the minor axis. Referring to FIG. 1, a clear maltese cross pattern appeared in the polarized image of the liquid crystal emulsion prepared in Example 1, thereby confirming the liquid crystal emulsion having optical anisotropy characteristics.

(2) Cyro-SEM observation

In order to confirm that the lamellar liquid crystal was well formed in the cosmetic prepared in Example 1, the prepared sample was observed with Cyro-SEM, and the results are shown in FIG. 2 . Referring to FIG. 2, it was confirmed that the liquid crystal emulsion of Example 1 had a multilayer lamellar structure.

Experimental example 3: Stability observation

This experiment was performed to determine whether the multi-layered lamellar liquid crystal structure was stably maintained while the liquid crystal emulsion composition of Example 1 prepared in Table 5 secured meta-stability when dropped in water, which is a continuous phase, like the form of the actual cosmetic composition. .

Therefore, 30.0% by weight of the composition of Example 1 was added to 70.0% by weight of water, and Sample 1 was prepared by simple stirring. And, the results of observation of its properties (transparency) and particle size for each time period are shown in Table 6.

At this time, the observation of the particle size of the present invention was measured using a particle size analyzer (Otsuka ELS-Z2, Otsuka Electronics, Japan).

As shown in Table 6, the liquid crystal emulsion composition containing 2.00% of the irritation-relieving complex according to the present invention showed excellent properties in terms of transparency and particle size even when dropped into water, and through this, the continuous phase was a water-based foundation And/or it was found that it is very easy to apply to color cosmetics.

division 0 weeks 1 week 2 weeks 4 weeks particle size 638.5 nm 787.2 nm 775.0 nm 824.8 nm Appearance (transparency) translucent translucent translucent translucent

Experimental example 4: Skin permeability test

In order to examine the effect of the lamellar liquid crystal emulsion composition (Example 1) of the present invention on the skin delivery of the skin irritation alleviation complex, the skin permeation amount was measured using a Franz diffusion cell, and the results are shown in FIG. 3 . As a control, an O/W emulsion (Comparative Example 1) containing 2.00 wt% of the alleviation complex and butylene glycol in which the complex was dissolved in 2.00% by weight were used. The results are shown by quantifying the amount of the alleviation complex after 24 hours. The amount of material present in the stratum corneum (Tape), the amount of material present in the epidermis and dermis excluding the stratum corneum (Skin), and the amount penetrating the skin (Transdermal) separated by . As a result of using the lamellar liquid crystal emulsion composition, the transdermal amount was similar to that of the control, but the amount in the epidermis and dermis (Skin) slightly increased, and the amount (Tape) in the stratum corneum increased significantly. .

As a result, the total skin absorption capacity of the control group, butylene glycol, was 8.2%, which was lower than that of the experimental group, and the O/W emulsion of Comparative Example 1 was 12.6%, which significantly improved skin permeability compared to the control group. In the case of the lamellar liquid crystal emulsion of Example 1, it was 14.0%, indicating more improved skin permeability compared to the O/W emulsion.

Through these results, it was confirmed that the active ingredient was delivered to the skin better than the control when the irritation-relieving complex was supported in the lamellar liquid crystal emulsion. Studies have shown that the lipid layer of the skin can be affected by an emulsion with a liquid crystal structure. When applied to the skin, the surfactant and fatty alcohol component of the liquid crystal emulsion interact with the intercellular lipid component of the stratum corneum and effectively fuse with the lipid of the stratum corneum. The skin penetration effect is increased. In addition, the water retention effect of the liquid crystal emulsion is superior to that of a general emulsion, and it hydrates the barrier of the stratum corneum of the skin to increase the skin penetration effect.

Experimental example 5: skin barrier recovery and erythema index improvement effect

As the test composition, the lamellar liquid crystal emulsion composition of Example 1 and the O/W emulsion composition of Comparative Example 1 were used. A total of 15 subjects were recruited in the trial, and 15 completed this clinical trial. The age distribution was between male and female subjects aged 26-41 years (mean 33.0±2.4 years) with healthy skin.

Subjects suitable for the purpose of this test were selected, and each test substance treated site and untreated control site were set on the forearm, and 5 μl/cm 2 of each was applied twice a day. Before application of the test substance, 0.5% sodium lauryl sulfate was treated for 16 hours, and at each time point 1, 2, and 3 days after treatment. Trans-epidermal Water Loss (TEWL) was measured with Tewameter TM210 (C+K, Germany), and erythema index was measured with Spectrophotometer CM-600d (Minolta, Japan). The obtained results are shown in FIGS. 4 and 5 below. Figure 4 is a graph showing the improvement in the amount of transdermal water loss in the treatment group and the untreated group treated with the liquid crystal emulsion composition of Example 1 and the O/W emulsion of Comparative Example 1. 5 is a graph showing the improvement of erythema in the treatment group and the untreated group treated with the liquid crystal emulsion composition of Example 1 and the O/W emulsion of Comparative Example 1.

Comparing the improvement rate of transdermal moisture loss after 3 days of application, compared to before use, O/W emulsion showed 72.9% improvement and liquid crystal emulsion 86.4%, showing a difference of 13.5% of improvement, which is liquid crystal compared to O/W emulsion. It can be seen that the effect of improving the skin barrier of the emulsion is greater.

Comparing the erythema improvement rate 3 days after application, the O/W emulsion showed an improvement of 43.9% and the liquid crystal emulsion 68.1% compared to before use, showing a difference of 24.2% of the improvement rate, which is that of the liquid crystal emulsion compared to the O/W emulsion. It can be seen that the erythema improvement effect is greater.

Experimental example 6: Moisture improvement effect

In order to evaluate the skin moisturizing effect of the lamellar liquid crystal cosmetic composition containing the irritation alleviation complex of the present invention, the skin to which each test substance of Example 1 and Comparative Example 1 was applied and the untreated group were subjected to moisture The retention capacity was measured as follows. Moisture retention capacity was measured in a constant temperature and humidity room at a room temperature of 22° C. and a relative humidity of 45%, and a certain amount (5 μl/cm 2) of Example 1 and Comparative Example 1 was applied to the inside of the forearms of 20 subjects, and prior to application, application The moisture content of the skin after 1 week and 2 weeks after application was measured. As a measuring device, a moisture content meter (corneometer CM820, Courage + Khazaka, Cologne, Germany) was used to measure the moisturizing power by measuring the skin's electric capacity according to the skin's moisture content. The results are shown in Table 7 below.

division Example 1 Comparative Example 1 no treatment before use 22.9 23.2 23.0 1 week after use 52.0 33.3 22.8 2 weeks after use 56.3 34.7 23.2

From Table 7, in the case of the liquid crystal emulsion containing the irritation-relieving complex of the present invention, the electrical conductivity of the skin is higher than that of Comparative Example 1 and the untreated group. Accordingly, it can be seen that the composition has an excellent moisture retention ability and has a moisturizing effect.

Experimental example 7: liquid crystal emulsion Primary stimulus evaluation (patch test)

When the lamellar liquid crystal cosmetic composition of the present invention is directly applied to human skin, a human skin patch test was performed to confirm whether it has an excellent skin irritation alleviation effect. The test subject visited a total of three times (before the start of the test, on the date of removal of the patch, and after 30 minutes after removal of the patch) to determine whether or not skin irritation occurred during this human application test period. At the first visit, a skin patch test was performed on the subject's back using a Finn chamber (100×10, EPITEST, Finland). For 15 test subjects, the sample was applied between the shoulder blade and the waistline with the spine on the back using a pin chamber.

After wiping the test site with 70% ethanol and drying it, 20 μl of a test sample prepared by adding 5.0% lactic acid according to Table 8 was dropped into a pin chamber at a time, and then placed on the test site and fixed. In Example 2, which was used as a test sample, 0.8% and 3.2% of C12-20 alkyl glucoside and C14-22 alcohol required to form a liquid crystal emulsion were added, respectively, and 2% of an alleviating complex was added. Comparative Example 2 in which the irritation relief complex was not added was tested together. In Example 3, 2% of glyceryl stearate and 2% of PEG-100 stearate were added to the oil phase forming an O/W emulsion, and 2% of the irritation relief complex was added. Comparative Example 3 in which the irritation relief complex was not added was tested together. The patch was applied to the skin for 24 hours. After the patch was removed, the test site was marked with a marking pen, and after 30 minutes, erythema and edema at the test site were observed using a magnifying glass (8MC-150, DAZOR, USA).

Raw material name Example 2 Comparative Example 2 Example 3 Comparative Example 3 glyceryl stearate - - 2.00 2.00 PEG-100 Stearate - - 2.00 2.00 C12-20 Alkyl Glucoside 0.80 0.80 - - C14-22 alcohol 3.20 3.20 - - Potassium Cetyl Phosphate 1.00 1.00 1.00 1.00 Ceramide-3 0.01 0.01 0.01 0.01 cholesterol 0.10 0.10 0.10 0.10 hydrogenated lecithin 0.20 0.20 0.20 0.20 Caprylic/Capric Triglycerides 25.00 25.00 25.00 25.00 behenyl alcohol 6.00 6.00 6.00 6.00 glycerin 25.00 25.00 25.00 25.00 Distilled water 30.97 32.97 30.97 32.97 lactic acid 5.00 5.00 5.00 5.00 Alleviation complex 2.00 - 2.00 - metal ion sequestrant 0.02 0.02 0.02 0.02 Napri Natural Preservative 0.70 0.70 0.70 0.70 Sum 100.00 100.00 100.00 100.00

The skin reaction was determined according to the regulations (Table 2) of the International Contact Dermatitis Research Group (ICDRG), and the average skin reactivity was calculated according to Equation 2, and the results are shown in Table 9.

The degree of skin irritation is judged as non-irritating if the stimulation rate is 0.0, non-irritating if 0.1-1.0, mild if 1.0-1.7, moderately irritating if 1.7-2.5, and strong if over 2.5.

composition skin irritation rate stimulus degree Example 2 0.87 unstimulated Comparative Example 2 2.17 central stimulus Example 3 1.53 mild stimulation Comparative Example 3 2.17 central stimulus

composition Stimulus relief rate (%) Example 2 59.91 Example 3 29.49

As can be seen from Experimental Example 7 of the present invention, it was confirmed that the liquid crystal emulsion and O/W emulsion formulations containing 5.0% (w/w) lactic acid caused strong irritation to the skin. For reference, it was confirmed that Examples 2 and 3 containing the irritation-relieving complex of the present invention had significantly lower skin irritation than Comparative Examples 2 and 3 that did not contain them. In particular, comparing the skin irritation rates of Examples 2 and 3, it can be seen that when the irritation relief complex of the present invention is contained in the lamellar liquid crystal cosmetic composition of Example 2, the irritation relief effect against the stimulation of lactic acid is further increased. could

Claims (6)

A cosmetic composition for relieving skin irritation in a lamellar liquid crystal type containing a skin irritation relief complex comprising madecassoside, allantoin and panthenol,
The skin irritation alleviation complex contains madecassoside, allantoin and panthenol in a weight ratio of 1-2: 4-5: 4-5,
The lamellar liquid crystal is an oil phase comprising 1 to 30% by weight of a nonionic surfactant, 1 to 10% by weight of a higher alcohol, and 0.1 to 10% by weight of a lipid component relative to the total weight of the cosmetic composition, 20 to 30% by weight of a polyol, skin irritation relief A cosmetic composition for alleviating skin irritation, characterized in that it is formed including an aqueous phase containing 0.1 to 10.0% by weight of the complex and the remaining amount of water. According to claim 1,
The skin irritation alleviation complex is a cosmetic composition for alleviating skin irritation contained in an amount of 0.1 to 10.0% by weight based on the total weight of the cosmetic composition. According to claim 1,
The nonionic surfactant is at least one selected from the group consisting of C12-20 alkylglucoside, potassium cetyl phosphate and sorbitan palmitate, and the lipid component is ceramide-3, cholesterol, lecithin and caprylic/capric triglyceride. At least one selected from the group consisting of, the higher alcohol is C14-22 alcohol and behenyl alcohol, a cosmetic composition for alleviating skin irritation. delete delete delete

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