KR102303811B1 - Cosmetic Composition for Skin Whitening, Moisturizing and Anti-Iching Containing Pseudoalteromonas Fermented of Enzyme Treated Pearls - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening, moisturizing and alleviating skin itchiness, comprising a fermented product of enzyme-treated pearls with pseudoalteromonas as an active ingredient. The fermented product obtained by fermenting the enzyme-treated pearls with Pseudoalteromonas is sterilized extracts obtained by treating pearls with proteolytic enzymes and then fermented with Pseudoalteromonas, a marine microorganism. The ingredients produced through the treatment enhance the fermentation effect and have excellent whitening effect, skin moisturizing effect and itch relief effect.

Description

A cosmetic composition for skin whitening, moisturizing and relieving skin itching containing fermented products of enzyme-treated pearls with Pseudoalteromonas as an active ingredient

The present invention relates to a cosmetic composition for skin whitening, moisturizing and alleviating skin itchiness, comprising a fermented product of enzyme-treated pearls with pseudoalteromonas as an active ingredient.

Several factors are involved in determining a person's skin color. There are factors such as the activity of melanocytes that make melanin pigment, the distribution of blood vessels, the thickness of the skin, and the presence or absence of pigments inside and outside the human body such as carotenoids and bilirubin. Among them, a representative factor is due to the exposure of the skin to ultraviolet rays. That is, when the skin is exposed to ultraviolet rays, tyrosinase is activated, which acts on tyrosine, a kind of amino acid present in the skin tissue, to generate dopa, and then through the oxidation process to generate dopaquinone again, the skin pigment A polymer called melanin is synthesized in melanosomes in the melanocytes, which are cells. This melanin is transferred to keratinocytes, which are keratinocytes of the skin, and reaches the skin surface by the keratinization process to protect the skin from UV rays.

Melanin is an essential UV protection agent for our human body and plays a role as an effective free radical scavenger to remove various radicals that modify biocomponents such as proteins, lipids, and nucleic acids. However, when melanin is locally synthesized excessively or the physiological function of the skin is deteriorated due to skin lesions and aging, melanin is deposited on the skin surface, causing melasma, freckles and various pigmentation.

As the cause and mechanism of skin blackening have been revealed, a method of reducing the production of melanin by mixing substances having an inhibitory effect on the activity of tyrosinase, which is involved in the skin blackening process, into cosmetics, or inhibiting some reactions during the process of melanin production It is commonly used to prevent skin darkening. Representative materials used for this purpose include plant extracts such as arbutin, ascorbic acid, hydroquinone, sagebrush extract and licorice extract.

However, in the case of arbutin, while it has an excellent tyrosinase activity inhibitory action, it has problems in stability such as discoloration and a decrease in potency due to changes over time when formulated in cosmetics, and there is a limitation in use due to high skin irritation. Ascorbic acid has a low tyrosinase activity inhibitory effect and is not suitable as a melanogenesis inhibitor due to low molecular stability. Hydroquinone has a high irritation to the skin, so there is a problem that its usability is limited in the current cosmetic formulation due to safety issues. In addition, most plant extracts exhibit a relatively high tyrosinase activity inhibitory effect only at high concentrations, and have problems such as almost no inhibitory effect at low concentrations. In particular, the existing inhibitors of melanin production have the biggest problem that the whitening effect is weak during clinical trials on actual human skin. Therefore, there is a demand for the development of a material that ensures stability and safety while exhibiting an excellent whitening effect in the human body, and thus, a whitening composition based on a natural product with few side effects is attracting attention.

As an organ that functions as a protective film that protects the living body from the external environment, the skin prevents the loss of biological components such as water and electrolytes in the human body, and at the same time prevents the intrusion of harmful substances from the outside. The structure of the skin is divided into three layers: the epidermis, the dermis, and the subcutaneous tissue in order from the outside.

The moisture content of the skin is about 70% in the dermis layer, but decreases toward the epidermis layer and becomes about 10% to 30% in the epidermis layer. When the moisture content of the epidermis layer is less than 10%, the skin becomes rough and the protective function of the body is lost, and aging occurs. From the outside, the epidermis is divided into four layers: the stratum corneum, granular layer, stratum corneum, and basal layer. The cells of the stratum corneum act like bricks, and the lipids between the cells act like mortar, forming a skin barrier.

In addition, a high concentration of Natural Moisturizing Factor (NMF) exists in the keratinocytes of a healthy person, which helps the skin to contain moisture. However, in spite of these functions of the skin, extrinsic aging caused by external environments such as indoor heating, polluted air and ultraviolet rays, intrinsic aging and loss of skin components due to physical and chemical stimuli such as friction, shaving and washing face An imbalance can lead to dry skin. To prevent this, the need for a skin moisturizer is important, and various studies are being conducted to confirm the skin moisturizing effect at the cellular level.

It is very important to properly maintain the moisture content of the stratum corneum in the skin. For this purpose, a component similar to sebum, an NMF component, or a moisturizing agent such as polyol is added to cosmetics. For example, as water-soluble polyols, glycerin and solitol having three or more hydroxyl groups (OH groups) show excellent moisturizing power, but are sticky and cause discomfort when used, and propylene glycol with two hydroxyl groups, 1,3-butylene Glycol, etc., may cause side effects on the skin. In addition, other natural moisturizing factors such as sodium pyrrolidone carboxylate (PCA-Na), sodium lactate, and urea have strong electrolytic properties, which impair the emulsion stability of cosmetics. Although it has the ability, there is a limit to its moisturizing ability, so it is necessary to develop a natural cosmetic composition to increase skin moisturizing power.

Itching refers to a condition of the skin that causes the urge to scratch the skin and causes discomfort. It is mainly induced by various stimuli such as stress, physical/chemical stimuli, and temperature/humidity changes. These symptoms are also accompanied by people with sensitive skin or skin diseases such as atopic dermatitis and contact dermatitis. It is known that itching and skin diseases are caused by inducing the secretion of various cytokines, including histamine and TNF-α, which mediate itch by activation of specific immune cells in the body. Currently, research on itch signal transduction is being actively conducted, and drugs for treating itch are being developed through regulation of signal transduction process. Recent studies have shown that specific C nerve fibers transmit itch stimuli to the central nervous system. Histamine activates neurons through histamine receptor 1 (H1), which induces the opening of TRPV1 (Transient receptor potential cation channel, subfamily V, member 1) ion channel, and BAM8-22 and chloroquine are TRPA1 (Transient receptor potential cation channel). channel, subfamily A, member 1) activates neurons through MrgprC11 (Mas-related G protein-coupled receptor C11) and MgprA3 (Mas-related G protein-coupled receptor A3), which induce channel opening Finally, it is transmitted to the cerebral cortex and causes itching. In addition, protease receptor, PAR-2 (Protease activated receptor 2), is activated by serine-protease such as trypsin, and calcitonin gene-related peptide (CGRP) and NO, TNF-α , histamine, and Substance-P are secreted to induce acute and chronic itch and inflammation, which is involved not only in atopic dermatitis but also in scalp itch and contact dermatitis symptoms. There is a need to develop a natural cosmetic composition that is hypoallergenic to the skin and can alleviate skin itchiness.

Pearl, which is called the treasure of the sea, is a kind of gemstone that contains calcium carbonate as its main ingredient, and has an elegant and beautiful color. Freshwater pearls are produced mainly in China, Akoya pearls in Japan, black pearls in Tahiti, and Namyang pearls in Indonesia. Pearl cultivation is carried out in very few cases in Korea. For the growth of microorganisms, P, S, Mg, K, Na, Ca, etc. are required a lot as inorganic elements, and Fe, Mn, Co, Zn, Cu, etc. are required in trace amounts. Since these are mostly involved in the activity of enzymes, can promote growth. When microorganisms grow, they produce amino acids. As the microorganisms grow, the production of amino acids also increases. Among the amino acids, there are amino acids such as methionine, histidine, and arginine that inhibit melanin production and show efficacy in skin whitening. As such, various physiologically active substances can be developed through microbial culture, but there are problems in the price of commercial media, the development of selective media, and the growth rate of microorganisms, and efforts are being made to solve these problems. On the other hand, pearls, which are called the king of natural gems, contain 20 kinds of minerals, amino acids, and physiologically active substances such as calcium. Among them, the mineral component is an essential nutrient for the growth of microorganisms and can promote the growth of microorganisms, but there is no research on this. However, there are not many methods that can dramatically shorten the culture time and activity of microorganisms, and they are not economical.

Korean Patent Registration No. 10-1980068 discloses a method for improving the flavor of fermented vinegar and a technique for a composition for improving skin condition including a pearl fermented product prepared therethrough.

An object of the present invention is to provide a cosmetic composition for skin whitening, skin moisturizing and skin itch relief using a fermented product obtained by fermenting enzyme-treated pearls with pseudoalteromonas. Specifically, including the fermented product of pearls treated with proteolytic enzyme (Protamex) with Pseudoalteromonas, it inhibits some reactions in the melanin production process, suppresses melanin production, and improves aquaporin expression. An object of the present invention is to provide a cosmetic composition capable of alleviating skin itching by inhibiting TRPV1 expression as well as improving skin moisture.

The above object is achieved by a cosmetic composition for skin whitening, skin moisturizing, and skin itch relief containing a fermented product obtained by fermenting enzyme-treated pearls with pseudoalteromonas as an active ingredient.

Preferably, the fermented product obtained by fermenting the enzyme-treated pearls with Pseudoalteromonas has a skin moisturizing effect by improving the expression of Aquaporin (AQP)-3, and has a skin itch relief effect by inhibiting the expression of TRPV1 characterized in that

Also preferably, the fermented product obtained by fermenting the enzyme-treated pearls with Pseudoalteromonas may be contained in an amount of 0.001 to 99.0% by weight based on the total weight of the cosmetic composition.

Also preferably, the enzyme may be a proteolytic enzyme.

In the present invention, by fermenting the enzyme-treated pearls with Pseudoalteromonas, a marine microorganism, and using the mineral components of pearls as nutrients, the growth rate of Pseudoalteromonas was promoted more than 5 times, and skin whitening, moisturizing, and skin itching relief It is possible to provide a cosmetic composition having an excellent effect on

1 is a fermented product obtained by fermenting enzyme-treated pearls with pseudoalteromonas (Example 1), a pseudoalteromonas culture in MA medium (Comparative Example 1), and a pseudoalteromonas culture in a mineral medium (comparative). Example 2) and a fermented product obtained by fermenting pearls with pseudoalteromonas (Comparative Example 3) shows OD values measured with a UV spectrophotometer.
2 is a fermented product obtained by fermenting enzyme-treated pearls with pseudoalteromonas (Example 1), a pseudoalteromonas culture in MA medium (Comparative Example 1), and a pseudoalteromonas culture in a mineral medium (comparative). Example 2) and a fermented product obtained by fermenting pearls with Pseudoalteromonas (Comparative Example 3) shows the expression rate of TRPV1.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and have the meanings as commonly understood by one of ordinary skill in the art in the relevant field of the present invention. In addition, although preferred methods and samples are described herein, similar or equivalent ones are also included in the scope of the present invention.

The term "about" refers to 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length varying by 4, 3, 2 or 1%.

Throughout this specification, unless the context requires otherwise, the terms "comprises" and "comprising" include, but are not limited to, a given step or element, or group of steps or elements, but any other step or element, or It is to be understood that a step or group of elements is not excluded.

Hereinafter, the present invention will be described in detail.

The present inventors reduce the culture time of microorganisms that produce physiologically active substances with excellent efficacy in skin whitening, skin moisturizing, and skin itch alleviation and increase the activity to have commercial advantages, Focusing on functional cosmetics containing natural products containing minerals, and studying the potential of various raw materials for skin whitening, moisturizing, and skin itch relief cosmetic composition, pearls containing abundant minerals were selected. The present invention was completed.

The present invention provides a cosmetic composition containing, as an active ingredient, a fermented product obtained by fermenting enzyme-treated pearls with Pseudoalteromonas.

In the present invention, "Pearl" is a hard mass formed in the body of pearl oysters, clams, abalone, etc., and calcium carbonate is the main component, and is a kind of jewel with elegant and beautiful color luster. Freshwater pearls are produced mainly in China, Akoya pearls in Japan, black pearls in Tahiti, and Namyang pearls in Indonesia. Pearl farming is carried out in very few cases in Korea. Pearl, which is called the king of natural gems, contains 20 kinds of minerals, amino acids, and physiologically active substances such as calcium.

The cosmetic composition according to the present invention may contain a fermented product obtained by fermenting enzyme-treated pearls with Pseudoalteromonas, and the enzyme-treated pearls may be those obtained by enzymatically treating pearls or pearl powder.

In the cosmetic composition according to the present invention, the fermented product obtained by fermenting the enzyme-treated pearls with Pseudoalteromonas is obtained by treating pearls or pearl powder with a proteolytic enzyme, preferably Protamex, and then fermenting it with a fermented strain. it could be

The fermented product obtained by fermenting the enzyme-treated pearls with Pseudoalteromonas may include those obtained by fermenting the pearls with enzymes and then fermenting them with marine microorganisms, Pseudoalteromonas sp.

In the present invention, ' Pseudoalteromonas sp. ) is a marine microorganism that lives in the Ross Sea, Antarctica, and contains essential amino acids, so it can provide a rich moisturizing effect to the skin and a good effect on skin whitening.

On the other hand, in the cosmetic composition of the present invention, the enzyme-treated pearl fermented with Pseudoalteromonas is preferably contained in an amount of 0.001 to 99.0% by weight based on the total weight of the cosmetic composition. Most preferably, it is good to contain 0.01 to 50% by weight based on the total weight of the cosmetic composition. When the content of the extract is less than 0.001% by weight, the skin whitening improvement effect does not appear, and when it exceeds 99.0% by weight, the increase in skin whitening, moisturizing, and skin itch alleviation effect for the content increase is insignificant, and safety in formulation And there is a problem in stability, and it is not economical.

On the other hand, the cosmetic composition of the present invention may include components commonly used in the cosmetic composition, for example, antioxidants, stabilizers, solubilizers, conventional adjuvants such as vitamins, pigments and fragrances, and a carrier.

On the other hand, the cosmetic composition of the present invention can be prepared in any formulation conventionally prepared in the art, for example, a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream, spray, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

On the other hand, in the cosmetic composition of the present invention, when the formulation is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, Talc or zinc oxide may be used.

On the other hand, in the cosmetic composition of the present invention, when the formulation is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, Benzyl benzoate, propylene glycol, 1,3-butylene glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan may be used.

On the other hand, in the cosmetic composition of the present invention, when the formulation is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester Suspending agents such as, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth may be used.

On the other hand, in the cosmetic composition of the present invention, when the formulation is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally propellants such as chlorofluorohydrocarbons, propane/butane or dimethyl ether.

On the other hand, in the cosmetic composition of the present invention, when the formulation is surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, sarcosinate, Fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester and the like can be used.

On the other hand, in the cosmetic composition of the present invention, in the case of a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation or soap, it may be applied to the skin and then wiped off, removed, or washed off with water. For example, the soap is liquid soap, powder soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream, cleansing lotions, cleansing waters and cleansing gels, but not limited thereto.

On the other hand, the cosmetic composition of the present invention may be used overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the skin condition or taste of the user.

Hereinafter, the content of the present invention will be described in more detail in the following examples, but the scope of the present invention is not limited to the following examples, and includes modifications of technical ideas equivalent thereto.

Preparation Example 1: Enzyme-treated pearl preparation and measurement of mineral content change

After purchasing pearls, pulverize the pearls and sterilize them at 121°C for 15 minutes. After sterilization, purified water was mixed so that 10 times of the pulverized product was added, and protamex, a proteolytic enzyme (Protamex, Novozyme Co, Bagsvaerd, Denmark), was added to 1% by weight of the pulverized product, followed by reaction at 60° C. for 2 hours. After the reaction, the enzyme was inactivated at 95° C. for 15 minutes to prepare an enzyme-treated pearl. The mineral content of the prepared enzyme-treated pearls was measured and shown in Table 1 below.

mineral Pearls after enzyme treatment Phosphorous (mg/100g) 0.40 mg/100 g Sodium (mg/100g) 7.58 mg/100g Calcium (mg/100g) 7.93 mg/100g Potassium (mg/100g) 4.67 mg/100g Magnesium (mg/100g) 0.41 mg/100 g Zinc (mg/100g) 0.02mg/100g

Referring to Table 1, it was confirmed that various kinds of minerals were present when pearls were enzymatically treated with Protamex.

Comparative Example 1: Pseudoalteromonas culture in MA (MARINE AGAR) medium

Peptone, 5.0 g, Yeast extract, 1.0 g, Ferric Citrate, 0.1 g, NaCl, 19.45 g, MgCl ₂ , 8.8 g, Na ₂ SO ₃ , 3.24 g, CaCl ₂ , 1.8 g, KCl, 0.55 g, NaHCO ₃ , 0.16 g, KBr, 0.08 g, SrCl ₂ , 0.034 g, H ₃ BO ₃ , 0.022 g, Na ₂ SiO ₃ , 0.004 g, NaF, 0.0024 g, NH ₄ NO ₃ , 0.0016 g, Na ₂ HPO ₄ , 0.008 g, Agar, and 15.0 g were mixed to prepare a commercial medium MA medium, followed by sterilization at 121° C. for 15 minutes. After the temperature of the medium was lowered to 35 ℃ , Pseudoalteromonas sp. 50,000 cfu/L of mycelium was added and cultured for 3 days in a fermenter under aerobic conditions at 32 ℃, pH6 and fermented. After fermentation, the fermented product was sterilized at 121° C. and 1.5 atm conditions for 15 minutes to kill bacteria, and then filtered using a 0.45 μm filtration membrane to obtain a culture.

Comparative Example 2: Pseudoalteromonas culture in mineral composition medium

Mix Phosphorous 0.40 mg, Sodium 7.58 mg, Calcium 7.93 mg, Potasium 4.67 mg, Magnesium 0.41 mg, Zinc 0.02 mg to make 100 g with purified water. After preparing the mineral medium, it was sterilized at 121°C for 15 minutes. After the temperature of the medium was lowered to 35 ℃ , Pseudoalteromonas sp. 50,000 cfu/L of mycelium was added and cultured for 3 days in a fermenter under aerobic conditions at 32 ℃, pH6 and fermented. After fermentation, the fermented product was sterilized at 121° C. and 1.5 atm conditions for 15 minutes to kill bacteria, and then filtered using a 0.45 μm filtration membrane to obtain a culture.

Comparative Example 3: Preparation of a fermented product obtained by fermenting pearls with pseudoalteromonas

After purchasing pearls, pulverize the pearls and sterilize them at 121°C for 15 minutes. After mixing 200 g of purified water , Pseudoalteromonas sp. 50,000 cfu/L of mycelium was added and cultured in a fermenter for 3 days under aerobic conditions at 32° C. and pH 6 and fermented. After fermentation, the fermented product was sterilized at 121° C. and 1.5 atm for 15 minutes to kill bacteria, and then filtered using a 0.45 μm filtration membrane. The fermentation filtrate was prepared and used by concentration according to the following experimental examples.

Example 1: Preparation of fermented product by fermenting enzyme-treated pearls with Pseudoalteromonas

50,000 cfu/L of Pseudoalteromonas sp. mycelium was added to 200 g of the enzyme-treated pearl prepared in Preparation Example 1, followed by culturing for 3 days in a fermenter under aerobic conditions at 32° C., pH 5 to 7, preferably pH 6. fermented. After fermentation, the fermented product was sterilized at 121° C. and 1.5 atm for 15 minutes to kill bacteria, and then filtered using a 0.45 μm filtration membrane. The fermentation filtrate was prepared and used by concentration according to the following experimental examples.

Experimental Example 1: Measurement of microbial activity and mineral content

Pseudoalteromonas culture in MA medium (Comparative Example 1), PseudoAlteromonas culture in mineral medium (Comparative Example 2), and fermented product in which pearls were inoculated with PseudoAlteromonas and fermented for 3 days (Comparative Example) 3), and the proteolytic enzyme (Protamex)-treated pearls were inoculated with Pseudoalteromonas and fermented for 3 days (Example 1) to measure the activity of each microorganism. The microbial activity was measured using a UV spectrophotometer (spectrophotometer) OD value. The results are shown in FIG. 1 .

Referring to FIG. 1, when the enzyme-treated pearls were inoculated with Pseudoalteromonas and fermented (Example 1), it was confirmed that the activity of Pseudoalteromonas was about twice higher than that of culturing in the existing MA medium.

In addition, the mineral content of Comparative Example 2, Comparative Example 3, and Example 1 was requested and analyzed by the Korea Polymer Testing Laboratory. The results are shown in Table 2. Referring to Table 2, in Example 1, phosphorus (Phosphorous), sodium (Sodium), calcium (Calcium), potassium (Potassium), magnesium (Magnesium), zinc (Zinc) was reduced, in particular, a decrease in sodium, calcium, potassium was big From this, it can be seen that Pseudoalteromonas consumes minerals as nutrients during the fermentation process.

mineral Comparative Example 2 Comparative Example 3 Example 1 Phosphorous 0 mg/100g 0 mg/100g 0 mg/100g sodium 2.58 mg/100g 2.61 mg/100g 2.21 mg/100 g Calcium 2.73mg/100g 2.56 mg/100g 2.08mg/100g Potassium 1.16 mg/100g 0.82 mg/100g 0.52 mg/100g Magnesium 0 mg/100g 0 mg/100g 0 mg/100g Zinc 0 mg/100g 0 mg/100g 0 mg/100g

In addition, the amino acid content of Comparative Example 1, Comparative Example 2, Comparative Example 3 and Example 1 was measured by requesting the Korea Polymer Testing Laboratory. The results are shown in Table 3. Referring to Table 3, it was possible to see a higher amino acid content when the enzyme-treated pearl of Example 1 was fermented with Pseudoalteromonas as compared with Comparative Example 1, Comparative Example 2, and Comparative Example 3. When pearls are fermented after enzyme treatment, not only minerals but also components produced after enzyme treatment act as carbon and nitrogen sources to promote the growth of microorganisms, thereby increasing the content of amino acids, which are physiologically active substances according to the fermentation of microorganisms.

amino acid Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 1 Tyrosine 0 mg/100g 0 mg/100g 0 mg/100g 0 mg/100g Glycine 3.02mg/100g 5.58 mg/100g 5.88 mg/100g 6.88 mg/100g Serine 1.10 mg/100g 2.61 mg/100g 2.71 mg/100g 3.61 mg/100g Alanine 2.58 mg/100g 3.16 mg/100g 4.58 mg/100g 5.16 mg/100g Glutamic Acid 4.01 mg/100 g 4.51 mg/100 g 6.01 mg/100 g 6.51 mg/100g Lysine 0.93 mg/100 g 2.35mg/100g 2.81 mg/100g 3.31 mg/100g leucine 2.13mg/100g 4.17 mg/100g 4.63 mg/100g 5.63 mg/100g Methionine 0.23 mg/100 g 1.02mg/100g 1.50 mg/100g 1.80 mg/100g Valine 2.01mg/100g 1.33mg/100g 2.33mg/100g 3.33mg/100g Arginine 2.04 mg/100g 2.25mg/100g 2.64mg/100g 3.64mg/100g Asparatic Acid 2.08mg/100g 3.31 mg/100g 3.68 mg/100g 4.38 mg/100g Isoleucine 3.02mg/100g 3.37 mg/100g 4.56 mg/100g 5.37 mg/100g Threonine 0.98 mg/100g 2.07 mg/100g 2.18mg/100g 2.38 mg/100g Phenylalanine 0 mg/100g 0 mg/100g 0 mg/100g 0 mg/100g Proline 0 mg/100g 0 mg/100g 0 mg/100g 0 mg/100g Histidine 1.82 mg/100 g 2.54 mg/100g 3.07 mg/100g 3.34 mg/100g

Experimental Example 1: Analysis of tyrosinase activity inhibition effect

In this experimental example, the tyrosinase activity inhibitory effect of Comparative Example 1, Comparative Example 2, Comparative Example 3 and Example 1 was confirmed. At this time, arbutin, a well-known whitening agent, was used as a comparative sample.

Tyrosinase is an enzyme that promotes the oxidation process of a substance called tyrosine in a living body to produce melanin. Tyrosinase was isolated and purified from mushrooms, and was purchased from Sigma and used. The substrate, L-tyrosine, was dissolved in 0.05 M sodium phosphate buffer (pH 6.8) to make a 0.1 mg/ml solution, and Pseudoalteromonas culture in commercial medium (MA), Pseudoalteromonas culture in mineral medium Water, fermented pearls with pseudoalteromonas, and enzyme-treated pearls with pseudoalteromonas were used after dissolving in 0.05 M sodium phosphate buffer (pH 6.8) to an appropriate concentration. 0.5 ml of L-tyrosine solution is placed in a test tube, and there is a pseudoalteromonas culture in commercial medium (MA), a pseudoalteromonas culture in mineral medium, a fermented product of pearls with pseudoalteromonas, an enzyme 0.5 ml of each sample solution of a fermented product obtained by fermenting treated pearls with Pseudoalteromonas was added and left in a thermostat at 37° C. for 10 minutes. Thereafter, 0.5 ml of 200 unit/ml tyrosinase was added and reacted at 37° C. for 10 minutes. The test tube containing the reaction solution was placed on ice to quench the reaction to stop the reaction, and then the absorbance at a wavelength of 475 nm was measured with a spectrophotometer. As a control, 0.5 ml of a buffer solution was used instead of commercial medium (MA), mineral medium, pearl fermentation, and enzyme-treated pearl fermentation. At this time, the activity inhibition rate (%) for tyrosinase was calculated by Equation 1 below, and the IC ₅₀ value when the tyrosinase activity was inhibited by 50% was measured for each whitening material. The results are shown in Table 2 below.

division IC ₅₀ (ug/ml) (concentration required for 50% inhibition of tyrosinase activity) Comparative Example 1 No inhibitory effect on activity at high concentrations Comparative Example 2 No inhibitory effect on activity at high concentrations Comparative Example 3 230.7 Example 1 165.1 arbutin 198.5

As can be seen in Table 4, the concentration inhibiting tyrosinase activity by 50% did not show efficacy in the Pseudoalteromonas culture in the MA medium of Comparative Example 1, and the enzyme-treated pearl of Example 1 was treated as pseudoaltero. Compared with the fermented product fermented with Monas 165.1 g/ml and arbutin was 198.5 μg/ml, it was confirmed that the tyrosinase activity inhibitory effect of the Pseudoalteromonas fermentation of the enzyme-treated pearls was significantly superior.

Experimental Example 2: Analysis of melanin production inhibitory effect using B16F10 melanin-forming cells

In this experimental example, in order to confirm the whitening effect of the fermented product of enzyme-treated pearls with Pseudoalteromonas, it was attempted to investigate the degree of inhibition of melanogenesis in B16F10 melanin-forming cells. At this time, arbutin, a well-known whitening agent, was used as a comparative sample.

The B16F10 melanin-forming cells used in this experimental example are mouse-derived cell bacteria, and were used after receiving from ATCC (American Type Culture Collection). ^{B16F10 melanin-forming cells were dispensed at a concentration of 2×10 5} per well in a 6-well plate, and dispensed at a concentration that did not induce toxicity after attaching the cells, and Comparative Examples 1 and 2 Comparative Examples 3 and 1 were treated and incubated for 72 hours. After culturing for 72 hours, the cells were detached with trypsin-EDTA, the number of cells was counted, and the cells were recovered by centrifugation. Quantification of intracellular melanin was performed by modifying the method of Lotan (Lotan: Cancer Res., 40: 3345-3350, 1980). After washing the cell pellet once with PBS, 1 ml of a homogenization buffer solution (50 mM sodium phosphate, pH6.8, 1% Triton X-100, 2 mM PMSF) was added and vortexed for 5 minutes to disrupt the cells. After dissolving the extracted melanin by adding 1N NaOH (10% DMSO) to the cell filtrate obtained by centrifugation (3,000rpm, 10 minutes), the absorbance of melanin was measured at 405 nm with a microplate reader, and then the melanin was quantified to sample Melanin production inhibition rate (%) was measured. The inhibition rate (%) of melanin production of B16F10 melanin-forming cells was calculated by Equation 2 below, and the results are shown in Table 5.

A: Amount of melanin in wells to which no sample was added

B: the amount of melanin in the well to which the sample was added

treatment concentration
(ug/ml) Melanin production inhibition rate (% of control) Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 1 untreated group 0.0 0.0 0.0 0.0 One 0.7 5.0 6.4 8.0 3 1.1 10.7 11.1 15.3 10 2.9 17.2 20.5 30.0 30 5.2 38.5 45.1 56.6 100 11.5 66.8 69.7 80.8 Arbutin 500 μg/ml 48.9

As a result of the experiment, as shown in Table 5, when the fermented product fermented with Pseudoalteromonas with enzyme-treated pearls was treated with 100 ug/ml, the melanin production inhibitory ability showed a better effect than when arbutin was treated with 500 ug/ml. Confirmed. Therefore, it can be seen that the whitening effect of the fermented product obtained by fermenting the enzyme-treated pearls with pseudoalteromonas is about 10 times superior to that of arbutin, which is a positive control.

Experimental Example 3: Aquaporin (Aquaporin, AQP)-3 expression pattern

HaCaT cells were seeded in a 6 well plate at 3 x 10 ⁵ cells/well, and cells were stabilized for one day in ^{a 37 o} C, 5% CO _{2 cell incubator.} Comparative Example 1, Comparative Example 2, Comparative Example 3 and Example 1 were treated by concentration and reacted for 6 hours, and then cells were collected using a scraper. Proteins were isolated using 1 x cell lysis buffer, and proteins were quantified by BCA assay, and then a certain amount of protein was electrophoresed on a 10% SDS-PAGE gel. The separated protein on the gel was transferred to a PVDF membrane and blocked for 1 hour using 5% skim milk. After washing 3 times with 1 x TTBS solution and reacting the primary antibody (AQP-3, Santa cruz , USA) at 4 ^o C overnight (over night), the HRP-conjugated secondary antibody was incubated at room temperature for 2 hours. reacted. Thereafter, the bands were detected with a Chemi Doc instrument after washing 3 times with 1 x TTBS and reacting the ECL solution. AQP-3 expression level was calculated by quantification based on the expression of beta-actin (β-actin) and quantified using Image J 1.47 software (Table 6). As a positive control, dexamethasone was used.

division density AQP-3 increase rate (%) Positive control (Dexamethasone) 1 μM 60.37 Comparative Example 1 25 μg/ml 2.50 50 μg/ml 9.58 100 μg/ml 25.70 Comparative Example 2 25 μg/ml 9.25 50 μg/ml 21.63 100 μg/ml 64.78 Comparative Example 3 25 μg/ml 11.62 50 μg/ml 25.71 100 μg/ml 70.80 Example 1 25 μg/ml 12.11 50 μg/ml 39.58 100 μg/ml 80.80

Referring to Table 6, the positive control dexamethasone increased the expression of AQP-3 by 60.37%, and Example 1 showed a significant effect from the 50 ug/ml concentration, and the positive control at the highest concentration of 100 ug/ml. , showed an increase rate of AQP-3 expression of 80.80% higher than that of Comparative Example 1, Comparative Example 2 and Comparative Example 3. Through this, it was confirmed that the fermented product obtained by fermenting the enzyme-treated pearl of the present invention with Pseudoalteromonas exhibits excellent moisturizing effect by increasing the expression of Aquaporin (AQP)-3.

Experimental Example 4: Confirmation of water holding capacity

Water holding capacity was measured by using a solution in which 1 wt% of hyaluronic acid (HA) was added as a comparative sample. The experiment was conducted at a constant temperature, and 20 ml of each of Comparative Example 1, Comparative Example 2, Comparative Example 3 and Example 1 was put in a Petri dish, and after 0, 2, 4, 6, 12 and 24 hours, precision Weighed with a scale and recorded

division hour 0H 2H 4H 6H 12H 24H vehicle
(1% HA) Sample amount (mg) 22187.4 21487.5 20475.6 19242.3 17458.5 15145.3 Decrease rate (%) 0 3.2 7.7 13.3 21.3 31.7 Comparative Example 1 Sample amount (mg) 21983.6 21020.7 20020.1 18684.5 16849.6 15022.1 Decrease rate (%) 0 2.9 6.6 12.5 19.2 30.6 Comparative Example 2 Sample amount (mg) 21983.6 21081.3 20182.1 19114.5 17812.6 16017.5 Decrease rate (%) 0 2.8 6.0 10.0 16.1 23.7 Comparative Example 3 Sample amount (mg) 21983.6 21440.7 20682.1 19684.5 18649.6 17022.1 Decrease rate (%) 0 2.5 5.9 10.5 15.2 22.6 Example 1 Sample amount (mg) 21983.6 21561.7 21038.0 20571.6 19735.4 18823.9 Decrease rate (%) 0 2.4 5.3 8.2 10.4 15.0

Looking at Table 7, compared to hyaluronic acid, which is known to have excellent moisturizing power, it was confirmed that the amount of evaporated moisture was small due to the excellent binding power of the fermented product obtained by fermenting the enzyme-treated pearls with Pseudoalteromonas. Through this, the fermented product obtained by fermenting the enzyme-treated pearl of the present invention with pseudoalteromonas is hyaluronic acid, the pseudoalteromonas culture in the MA medium of Comparative Example 1, and the pseudoalteromonas culture in the mineral medium of Comparative Example 2 It was confirmed that water and the pearl of Comparative Example 3 had a superior water retention ability than the fermented product with Pseudoalteromonas and exhibited an excellent moisturizing effect.

Experimental Example 5: TRPV1 expression inhibition evaluation

Human skin fibroblasts (Human fibroblast) were aliquoted in a 6-well plate (Nunc, Denmark) at ^{a concentration of 2 × 10 5} cells/well, 37 ℃, 5% CO ₂ Conditions were cultured for 24 hours. TRPV1 expression was induced by treatment with capsaicin (Capsaicin, 100 nM), and a buffer solution was used for the control group. The Comparative Example 1, Comparative Example 2, Comparative Example 3 and Example 1 were treated at concentrations of 30 and 100 ug/ml, respectively. After processing the sample and incubating for 6 hours, the cells were lysed with 1X cell lysis buffer after washing twice with PBS (Phosphate buffer saline) buffer. The lysed cells were placed in a 1.5 ml microtube and centrifuged at 12,000 rpm for 15 minutes, and the supernatant was recovered to separate proteins. The isolated protein was quantified by the BCA assay method, and SDS-PAGE was performed using an SDS-polyacrylamide gel. After electrophoresis, the protein was transferred from the gel to the PVDF membrane for 90 minutes and blocked with 5% skim milk for about 1 hour. As the primary antibody, TRPV1 antibody was used at a ratio of 1:1000 and reacted at 4°C for 24 hours. After the primary antibody reaction, 1X TBS-T buffer was used to wash 4 times for 10 minutes each, and the secondary antibody was immediately reacted at room temperature for 1 hour. After the reaction was completed, the band was confirmed using an ECL detection kit after washing 4 times for 10 minutes each. Band analysis and numerical values were shown using Chemi DocTM XRS+ machine and Image J 1.47 software, respectively. The value of TRPV1 was expressed as a percentage compared to the beta-actin (β-actin) value. Statistical significance was analyzed using Student`s t- test, and the level of significance was expressed as *p < 0.05 (FIG. 2).

As a result of the experiment, referring to FIG. 2, the composition containing the fermented product (Example 1) obtained by fermenting the enzyme-treated pearls of the present invention with Pseudoalteromonas cultured in MA medium (Comparative Example 1), The expression of TRPV1 was inhibited in a concentration-dependent manner than a composition containing a pseudoalteromonas culture in a mineral medium (Comparative Example 2) and a fermented product obtained by fermenting pearls with pseudoalteromonas (Comparative Example 3). TRPV1 is present in sensory nerves, and when activated, it secretes itch-inducing substances.

Formulation Example: Preparation of Cosmetics Containing Enzyme-treated Pearl Pseudoalteromonas Fermented Product

A cosmetic composition containing 3.0 wt% of the preparation according to Comparative Example 1, Comparative Example 2, Comparative Example 3 and Example 1 was prepared in the composition of Table 8 below, and Formulation Example 1, Formulation Example 2, Formulation Example 3 and Formulation Examples 4 was called. On the other hand, in order to confirm the efficacy, the cosmetic composition not containing the fermented product is shown in Table 8 as Comparative Formulation Example 1.

division
ingredient Formulation Example 1
content (wt%) Formulation Example 2
content (wt%) Formulation Example 3
content (wt%) Formulation Example 4
content (wt%) Comparative Formulation Example 1
content (wt%)

A Comparative Example 1 3.0 - - - - Comparative Example 2 - 3.0 - - - Comparative Example 3 - - 3.0 - - Example 1 - - - 3.0 - arbutin - - - - 3.0 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 Purified water to 100 to 100 to 100 to 100 to 100




B cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 1.5 1.5 microcrystallin 0.7 0.7 0.7 0.7 0.7 squalane 5.0 5.0 5.0 5.0 5.0 liquid paraffin 3.0 3.0 3.0 3.0 3.0 trioctanoin 5.0 5.0 5.0 5.0 5.0 polysorbate 1.2 1.2 1.2 1.2 1.2 Sorbitan Stearate 0.5 0.5 0.5 0.5 0.5 Tocopheryl Acetate 0.2 0.2 0.2 0.2 0.2 cyclomethicone 3.0 3.0 3.0 3.0 3.0 BHT 0.05 0.05 0.05 0.05 0.05 C fragrance, preservative appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount

Experimental Example 3: Analysis of Whitening Efficacy of Skin Whitening Cosmetic Composition

In this experimental example, the pseudoalteromonas culture in the MA medium, the pseudoalteromonas culture in the mineral medium, the fermented product obtained by fermenting pearls with pseudoalteromonas, and the enzyme-treated pearls were fermented with pseudoalteromonas. Whitening of a cosmetic composition for skin whitening containing a fermented product as an active ingredient (Formulation Example 1, Formulation Example 2, Formulation Example 3 and Formulation Example 4) and a cosmetic composition for skin whitening containing arbutin of Comparative Formulation Example 1 as an active ingredient We wanted to investigate the efficacy.

For the clinical trial, 100 healthy adult women with pigmentation were randomly divided into 5 groups (A, B, C, D, E) of 20 each, and then the cosmetic composition of Formulation Example 1 was administered to group A. , the cosmetic composition of Formulation Example 2 for group B, the cosmetic composition of Formulation Example 3 for group C, the cosmetic composition of Formulation Example 4 for group D, and Comparative Formulation Example 1 for group E The cosmetic composition was applied twice a day, respectively, for 8 weeks. Thereafter, the change in skin color is measured using a spectrophotometer (Minolta CM600-d) to measure the change in color brightness (ΔL), and objective visual observation by a plurality of experts and subjective visual observation by a subject are performed to achieve whitening effect was measured. The results are shown in Table 9 below. At this time, the degree of whitening efficacy was classified and evaluated according to the following whitening efficacy evaluation criteria.

<Evaluation criteria for whitening efficacy>

-3: very bad -2: bad -1: slightly worse 0: no change

1: slightly improved 2: improved 3: greatly improved

subject Change in skin color brightness (ΔL) Objective Assessment of Experts (ΔL) Subject's subjective evaluation (ΔL) A
group B
group C
group D
group E
group A
group B
group C
group D
group E
group A
group B
group C
group D
group E
group One 2 2 2 2 One 2 2 2 2 0 One 2 2 2 0 2 One One 2 2 One One One One 2 One 0 One 2 2 One 3 One 2 2 2 0 One 2 2 2 0 One 2 One One 0 4 One 2 2 2 One One 2 2 2 0 One 2 2 2 0 5 One 2 2 2 One One 2 2 2 One One One One 2 One 6 One 2 2 2 One One 2 2 2 One 0 One One 2 One 7 0 One 2 2 One 0 One One One One One 2 2 2 One 8 0 2 2 2 0 0 2 2 2 0 2 2 2 2 0 9 2 2 2 2 0 2 2 2 2 0 One 0 One One 0 10 One One One One 0 One One One 2 0 One One One 2 0 11 One 2 2 2 One One 2 2 2 One 2 One One 2 One 12 2 2 2 2 One 2 2 2 2 One 0 One One 2 One 13 2 2 2 2 One 2 2 2 2 One One One One One One 14 One 2 2 2 0 One One One One 0 2 2 2 2 0 15 One One One One One One One One One 0 One 2 2 2 0 16 One 2 2 2 2 One 2 2 2 2 One 2 2 2 2 17 One 2 2 2 0 One One One 2 0 0 0 0 One 0 18 One 2 2 2 One One 2 2 2 One One One 2 2 0 19 2 One One 2 One 2 One One 2 One 0 One One 2 One 20 One 2 2 2 One One 2 2 2 One One 2 2 2 2 average 1.2 1.8 1.8 1.9 0.8 1.2 1.6 1.6 1.9 0.6 0.9 1.4 1.5 1.8 0.6

As a result of the experiment, the average ΔL value of the group D coated with the cosmetic composition (Formulation Example 4) containing the fermented product of the enzyme-treated pearl of Example 1 with Pseudoalteromonas was 1.9 (p<0.05), which was the best and the average ΔL value of group A to which the skin whitening cosmetic composition (Formulation Example 1) containing the Pseudoalteromonas culture in the MA medium of Comparative Example 1 was applied was 1.2 (p<0.05), and that of Comparative Example 2 The average ΔL value of group B to which the skin whitening cosmetic composition (Formulation Example 2) containing the pseudoalteromonas culture in mineral medium was applied was 1.8 (p<0.05), and the pearl of Comparative Example 3 was treated with pseudoalteromonas The average ΔL value of group C to which the cosmetic composition containing the fermented product (Formulation Example 3) was applied was 1.8 (p<0.05). On the other hand, the average ΔL value of group E to which the skin lightening cosmetic composition containing arbutin (Comparative Formulation Example 1) was applied was 0.8 (p<0.05).

On the other hand, in the whitening evaluation by the expert, the A group was 1.2, the B group 1.6, the C group 1.6, the D group 1.9, and the E group 0.6, and in the whitening evaluation by the subject, the A group was 0.9 , group B was 1.4, group C was 1.5, group D was 1.8, and group E was 0.6. Through this, it was confirmed that the skin whitening cosmetic composition containing the fermented product obtained by fermenting the enzyme-treated pearls of the present invention with Pseudoalteromonas as an active ingredient was more excellent in whitening efficacy.

Preparation Example 2: Preparation of Cosmetics Containing Enzyme-treated Pearl Pseudoalteromonas Fermented Product

Cosmetics (Formulation Examples 5 to 8) each including Comparative Example 1, Comparative Example 2, Comparative Example 3 and Example 1 were prepared, and for comparison of efficacy, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Examples A cosmetic composition containing glycerin as a moisturizer instead of 1 (Comparative Formulation Example 2) and a cosmetic composition containing neither an extract nor a moisturizer (Comparative Formulation Example 3) are shown in Table 10 below.

division ingredient Formulation Example 5 Formulation Example 6 Formulation Example 7 Formulation example
8 comparison
Formulation Example 2 comparison
Formulation Example 3 A Comparative Example 1 5.0 - - - - - Comparative Example 2 - 5.0 - - - - Comparative Example 3 - - 5.0 - - Example 1 - - - 5.0 - - glycerin - - - - 5.0 - Butylene Glycol - - - - 5.0 - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 Purified water to 100 to 100 to 100 to 100 to 100 to 100 B cetosteayl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 microcrystallin 0.7 0.7 0.7 0.7 0.7 0.7 squal 5.0 5.0 5.0 5.0 5.0 5.0 liquid paraffin 3.0 3.0 3.0 3.0 3.0 3.0 trioctanoin 5.0 5.0 5.0 5.0 5.0 5.0 polysorbate 1.2 1.2 1.2 1.2 1.2 1.2 Sorbitan Stearate 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl Acetate 0.2 0.2 0.2 0.2 0.2 0.2 cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 BHT 0.05 0.05 0.05 0.05 0.05 0.05 C fragrance, preservative appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount

Experimental Example 4: Confirmation of skin itching (scratches) inhibitory effect

Formulation Example 5 containing Pseudoalteromonas culture in MA medium, Formulation Example 6 containing Pseudoalteromonas culture in mineral medium, each of 20 experimental groups for 100 skin disease patients with atopic lesions , Formulation Example 7 containing a fermented product of pearls fermented with Pseudoalteromonas and Formulation Example 8 treatment group containing a fermented product of fermented pearls with enzyme-treated Pseudoalteromonas, Cosmetic composition comprising glycerin as a moisturizing agent ( Comparative Formulation Example 2) Treatment group, a cosmetic composition that does not contain any fermented product or moisturizer (Comparative Formulation Example 3) was divided into 6 groups and the number of scratches was observed by taking a video for 3 hours after each application. The experimental results are shown in Table 11 below.

division Formulation Example 5 Formulation Example 6 Formulation Example 7 Formulation Example 8 Comparative Formulation Example 2 Comparative Formulation Example 3 Number of scratches/h 21 10 6 2 24 57

As a result of the experiment, the number of skin scrapings was significantly lower in Formulation Example 8 containing the fermented product obtained by fermenting enzyme-treated pearls with Pseudoalteromonas compared to those with skin diseases to which the composition of Comparative Formulation Example 3 was applied. Formulation Example 5 containing the Alteromonas culture showed similar results to Comparative Formulation Example 2 containing glycerin as a moisturizer

So far, specific embodiments of the present invention have been described. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (7)

A fermented product obtained by fermenting enzyme-treated pearls with pseudoalteromonas as an active ingredient, the fermented product is contained in an amount of 0.001 to 99.0% by weight based on the total weight of the cosmetic composition, and the enzyme is a proteolytic enzyme, a cosmetic for skin whitening composition. A fermented product obtained by fermenting enzyme-treated pearls with pseudoalteromonas as an active ingredient, the fermented product is contained in an amount of 0.001 to 99.0% by weight based on the total weight of the cosmetic composition, and the enzyme is a proteolytic enzyme, skin moisturizing cosmetic composition. It contains a fermented product obtained by fermenting enzyme-treated pearls with Pseudoalteromonas as an active ingredient, the fermented product is contained in an amount of 0.001 to 99.0% by weight based on the total weight of the cosmetic composition, and the enzyme is a proteolytic enzyme, for relieving skin itching cosmetic composition. 3. The method of claim 2,
The fermented product obtained by fermenting the enzyme-treated pearls with pseudoalteromonas is a cosmetic composition for moisturizing the skin, which improves the expression of aquaporin (AQP)-3 and has a skin moisturizing effect. 4. The method of claim 3,
The fermented product obtained by fermenting the enzyme-treated pearls with Pseudoalteromonas is a cosmetic composition for alleviating skin itchiness, characterized in that it inhibits TRPV1 expression. delete delete

Images