KR102297300B1 - 뎅기 바이러스 약독주를 뱅크화한 생바이러스, 및 그것들을 항원으로 하는 뎅기 백신 - Google Patents

뎅기 바이러스 약독주를 뱅크화한 생바이러스, 및 그것들을 항원으로 하는 뎅기 백신 Download PDF

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KR102297300B1
KR102297300B1 KR1020187017891A KR20187017891A KR102297300B1 KR 102297300 B1 KR102297300 B1 KR 102297300B1 KR 1020187017891 A KR1020187017891 A KR 1020187017891A KR 20187017891 A KR20187017891 A KR 20187017891A KR 102297300 B1 KR102297300 B1 KR 102297300B1
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켄고 소노다
야스히코 신무라
스스무 야마오리
모토하루 아베
신이치 마루노
쇼타 타카기
야스히사 하야시
카즈히사 가메야마
카즈히사 가메야마
푸사에 코무라
아키 푸쿠다
나오미 우치다
마사야 요시무라
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케이엠 바이올로직스 가부시키가이샤
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Abstract

4종의 모든 뎅기 바이러스 혈청형에 대한 중화 항체 응답을 단회투여로 유도하고, 일정량 이상의 바이러스 혈증을 발생하지 않는 안정성이 높은 뎅기 백신을 찾아냈다. 약효(중화 항체 응답)와 안전성(바이러스혈증)의 성적이 모두 뛰어난 4가 뎅기 바이러스 제제를 제공한다.

Description

뎅기 바이러스 약독주를 뱅크화한 생바이러스, 및 그것들을 항원으로 하는 뎅기 백신
본 발명은 약독화 뎅기 바이러스를 포함하는 뎅기 백신에 관한 것이다.
뎅기 바이러스 감염증은 특정한 감염증으로서는 세계 3대 감염증(AIDS, 말라리아, 결핵) 다음으로 발증자가 많고, 세계규모에서 만연하고 있다.
뎅기 바이러스는 플라비바이러스과에 속하는 싱글 체인 RNA 바이러스이며, 항원성으로 구별되는 4종의 혈청형이 존재한다. 어떤 종류의 혈청형 뎅기 바이러스에 감염되면, 획득 면역에 의해 동일 혈청형의 뎅기 바이러스에는 거의 평생에 걸쳐서 재감염되는 경우가 없다고 하지만, 한편으로 다른 혈청형의 바이러스에 대한 교차 보호는 길어도 12주 정도밖에 계속되지 않기 때문에, 2회 이상의 뎅기 바이러스 감염이 발생할 수 있다. 특히, 죽음에 이르는 경우가 있는 중독 병태인 「뎅기 출혈열」이나 「뎅기 쇼크 증후근」은 2회째 이후의 감염 시에 발생하기 쉽다는 것이 알려져 있다.
뎅기 바이러스 감염증에는 확립된 치료법은 없고, 예방법도 한정적이다. 기타, 감염 대책으로서는 뎅기 바이러스의 숙주인 모기의 구제나 환경정비, 개인 레벨의 대책으로서 기피제의 사용이나 적절한 복장에 관한 지도에 의지하고 있는 것이 실상이다. 그러나 이것들은 비용면이나 효과면에서 충분하다고 말할 수 없고, 폭넓은 연령층에 접종이 가능하며, 또 뛰어난 유효성을 나타내는 백신에 의한 감염 방지 또는 발증 예방이 소망되고 있다.
유럽 특허 제1159968호 일본 특허 제5197362호 일본 특허 제5075120호 국제공개특허 WO2007/141259 국제공개특허 WO2000/057910
Safety and immunogenicity of tetravalent live-attenuated dengue vaccines in Thai adult volunteers: role of serotype concentration, ratio, and multiple doses, Am. J. Trop MedImmune Hyg., 2002; 66(3):264-72. Dengue vaccines: recent developments, ongoing challenges and current candidates, Expert Rev Vaccines., 2013; 12(8):933-53. Efficacy of a tetravalent chimeric dengue vaccine (DENVax) in Cynomolgus macaque., Am J Trop Med Hyg., 2011; 84(6):978-87. Recombinant, live-attenuated tetravalent dengue virus vaccine formulations induce a balanced, broad, and protective neutralizing antibody response against each of the four serotypes in rhesus monkeys., J Virol, 2005; 79(9):5516-28. Evaluation of Interferences between Dengue Vaccine Serotypes in a Monkey Model, Am J Trop Med Hyg., 2009; 80(2):302-11. Protection of Rhesus monkeys against dengue virus challenge after tetravalent live attenuated dengue virus vaccination., J Infect Dis., 2006; 193(12):1658-65.
상술한 바와 같이, 첫 감염과는 다른 혈청형에 의한 2회째 이후의 감염에 의해 증상이 중독화되는 리스크가 높다고 생각되고 있기 때문에, 동시에 4종의 모든 혈청형의 뎅기 바이러스에 대한 방어능을 부여할 수 있는 4가 백신이 소망되고 있다. 그렇지만, 수 십년에 걸치는 백신개발에 대한 대처가 이루어졌음에도 불구하고, 4종의 모든 혈청형에 대해서 뛰어난 유효성을 나타내는 백신은 존재하지 않는다.
뎅기 바이러스 게놈은 C 단백질, M 단백질, E 단백질 등의 구조 단백질과, 바이러스 입자를 구성하지 않고 복제 시에 작용하는 7개의 비구조(NS) 단백질을 코딩하고 있다. 따라서, 다른 바이러스나 특정 혈청형의 뎅기 바이러스를 백본으로 하는 유전자 재조합 키메라 생백신, 또는, 불활화 전체 입자 백신이나 서브 유닛 백신, DNA 백신 등은 뎅기 유래 컴포넌트를 결여하고 있어, 뎅기 바이러스의 자연 감염에 의해 생체 내에 발생하는 여러 가지 면역항원 가운데의 일부를 제시하는 것에 지나지 않는다.
한편, 자연숙주와는 다른 세포에서 계대 순화해서 숙주세포 돌연변이를 획득한 약독화 생백신은 홍역, 풍진, 유행성 이하선염, 소아마비 등에서 실적이 있는 바와 같이, 자연 감염 시와 동일한 면역응답을 유도할 수 있기 때문에, 뎅기 바이러스 감염증에 있어서도 높은 유효성을 기대할 수 있다.
현재 개발 중임이 보고되고 있는 또는 출시된 뎅기 백신은 복수 회 투여함으로써 4종의 모든 혈청형의 뎅기 바이러스에 대한 중화 항체를 유도시키는 것이 가능하지만, 4종의 모든 혈청형에 대한 중화 항체가 획득될 때까지 기간을 필요로 하고, 유행지역에서는 그 기간 동안에 자연 감염될 리스크가 있어, 단회 투여에 의해 4종의 모든 혈청형에 대한 방어면역을 부여할 수 있는 백신이 보다 유용성이 높다고 말할 수가 있다.
뎅기 바이러스 백신의 조제에 있어서, 각 혈청형의 주 및 용량의 혼합 조성의 선정은 용이하지 않다. 단가(mono-valent)로 투여했을 경우에 높은 중화 항체를 유도 가능하다고 해도, 4가 혼합해서 투여하면 각 혈청형의 뎅기 바이러스에 대한 중화 항체 레벨이 균일하게 되지 않는다는(간섭 작용) 문제가 있다. 또, 불충분한 중화 활성(교차반응, 결합) 항체에 의한 항체 의존성 감염 증강(ADE; Antibody-Dependent Enhancement)의 문제도 있다.
기 승인약 또는 임상단계에 있는 주요한 개발품은 Sanofi의 ChimeriVax-DEN(Dengvaxia(상표명)), Takeda의 DENVax(상표명) 및 Butantan의 TetraVax(상표명)의 3개로, 모두 유전자 재조합 키메라 생백신이다. Sanofi의 Dengvaxia는 황열 백신주 YF-17D를 백본으로 하는 유전자 재조합 키메라 생백신이고, Mexico, 브라질 및 필리핀에 3개국에서 9세에서 45세를 대상으로 6개월 간격 3회 접종의 용법으로 승인되고 있다.
Dengvaxia의 Phase 3 시험은 동남아시아 5개국에 있어서, 2세에서 15세의 연령층의 약 1만명, 및 남미 5개국에서 9∼16세의 연령층의 약 2만명을 피험자로 해서 실시되었다. 그 결과, 유효성은 6할 정도로 그치고, 특히 혈청형 2에 대한 유효성은 약 4할로 낮아 충분하지 않았다. 또, 뎅기 기존 면역이 없는 피험자에 대한 유효성은 약 4할이었다. 또, Phase 3 시험의 장기 follow-up 및 태국에서의 Phase 2b시험의 장기 follow-up에서의 결과에서는 3년째의 시점에서 9세 미만의 연령층에서의 입원 리스크가 비접종 그룹에 대해서 1.6배로 높게 되어 있고, 특히 5세 이하에서는 약 5배로 높게 되어 있었다. 이 점이 Vaccine Enhancement이 아닌가라고 문제시 되고 있다. 유효성이 낮은 원인으로서는 뎅기 유래 컴포넌트의 부족이나 유행주의 돌연변이 등이 고찰되고 있다. 따라서, 뎅기 유래 컴포넌트를 전부 암시, 또, 적극적인 클로닝을 실시하고 있지 않고 바이러스의 아집단(quasispecies)을 포함하는 계대 순화 생백신이라면 더 높은 효과를 기대할 수 있다.
본 발명자들은 상기 과제를 해결하기 위해서 예의 연구한 결과, 약효와 안전성의 성적이 모두 뛰어난 약독화 4가 뎅기 생바이러스 백신을 발견했다. 또, 단회 투여해도, 복수 회 투여해도 약효와 안전성의 성적이 뛰어난 약독화 4가 뎅기 생바이러스 백신을 발견했다.
야생형의 뎅기 바이러스(혈청형 1∼4)를, 초기 개 신장(PDK; Primary Dog Kidney) 세포나 초기 녹색 원숭이 신장(PGMK; Primary Green Monkey Kidney) 세포에서 계대해서 수득한 약독주가 알려져 있다. 본 발명은 추가로, 아프리카 녹색 원숭이 신장(Vero) 세포에서 계대하는 것에 의해서 수득되는 약독화 뎅기 바이러스, 및, 그것들을 뱅크화한 생바이러스를 항원으로 포함하는 약독화 뎅기 바이러스 백신을 제공한다.
따라서 본 발명은 이하를 포함한다.
[1] 차세대 시퀀서를 사용해서 해석한 서열번호 1의 아미노산 서열을 가지는 혈청형 1 뎅기 바이러스 친주 03135와의 상동성이 95% 이상 있고, 그 친주의 483 위치, 568 위치, 1663 위치의 어느 하나의 아미노산이 완전하게 돌연변이되어 있으며, 추가로 이하의 (1)∼(4)의 어느 하나 이상의 돌연변이를 가지는 약독화 혈청형 1 뎅기 바이러스;
(1) K482E 또는 K482E/K
(2) K484R 또는 K484R/K
(3) I/T2353T
(4) A2364T, 또는 A2364T/A
[2] 상기 [1]에서, 서열번호 1로 나타내는 친주 03135의 아미노산 서열과의 상동성이 97% 이상 있는 약독화 혈청형 1 뎅기 바이러스.
[3] 상기 [1]에서, 서열번호 1로 나타내는 친주 03135의 아미노산 서열과의 상동성이 99% 이상 있는 약독화 혈청형 1 뎅기 바이러스.
[4] 상기 [1] 내지 [3] 중 어느 하나에서, 483 위치, 568 위치, 및 1663 위치의 아미노산이 완전하게 돌연변이되어 있는 약독화 혈청형 1 뎅기 바이러스.
[5] 상기 [4]에서, 483 위치의 돌연변이가 E483K인 약독화 혈청형 1 뎅기 바이러스.
[6] 상기 [4]에서, 568 위치의 돌연변이가 K568R인 약독화 혈청형 1 뎅기 바이러스.
[7] 상기 [4]에서, 1663 위치의 돌연변이가 N1663K인 약독화 혈청형 1 뎅기 바이러스.
[8] 상기 [4]에서, 483 위치의 돌연변이가 E483K이고, 568 위치의 돌연변이가 K568R이고, 1663 위치의 돌연변이가 N1663K인 약독화 혈청형 1 뎅기 바이러스.
[9] 상기 [1] 내지 [8]중 어느 하나에서, 상기 (1)∼(4)의 모든 돌연변이를 가지는 약독화 혈청형 1 뎅기 바이러스.
[10] 차세대 시퀀서를 사용해서 해석한 서열번호 2의 아미노산 서열을 가지는 혈청형 2 뎅기 바이러스 친주 99345와의 상동성이 95% 이상 있고, 그 친주의 143 위치, 400 위치, 1102 위치, 1308 위치, 1654 위치의 어느 하나의 아미노산이 완전하게 돌연변이되어 있으며, 추가로 이하의 (5) 또는 (6)의 돌연변이를 가지는 약독화 혈청형 2 뎅기 바이러스;
(5) P2347L 또는 P2347P/L
(6) T2828M 또는 T2828T/M
[11] 상기 [10]에서, 서열번호 2로 나타내는 친주 99345의 아미노산 서열과의 상동성이 97% 이상 있는 약독화 혈청형 2 뎅기 바이러스.
[12] 상기 [10]에서, 서열번호 2로 나타내는 친주 99345의 아미노산 서열과의 상동성이 99% 이상 있는 약독화 혈청형 2 뎅기 바이러스.
[13] 상기 [10] 내지 [12] 중 어느 하나에서, 143 위치, 400 위치, 1102 위치, 1308 위치, 및 1654 위치의 아미노산이 완전하게 돌연변이되어 있는 약독화 혈청형 2 뎅기 바이러스.
[14] 상기 [13]에서, 143 위치의 돌연변이가 D143N인 약독화 혈청형 2 뎅기 바이러스.
[15] 상기 [13]에서, 400 위치의 돌연변이가 T400K인 약독화 혈청형 2 뎅기 바이러스.
[16] 상기 [13]에서, 1102 위치의 돌연변이가 D1102N인 약독화 혈청형 2 뎅기 바이러스.
[17] 상기 [13]에서, 1308 위치의 돌연변이가 L1308F인 약독화 혈청형 2 뎅기 바이러스.
[18] 상기 [13]에서, 1654 위치의 돌연변이가 E1654K인 약독화 혈청형 2 뎅기 바이러스.
[19] 상기 [13]에서, 143 위치의 돌연변이가 D143N이며, 400 위치의 돌연변이가 T400K이고, 1102 위치의 돌연변이가 D1102N이고, 1308 위치의 돌연변이가 L1308F이고, 1654 위치의 돌연변이가 E1654K인 약독화 혈청형 2 뎅기 바이러스.
[20] 상기 [10] 내지 [19] 중 어느 하나에서, 상기 (5) 및 (6)의 돌연변이를 가지는 약독화 혈청형 2 뎅기 바이러스.
[21] 차세대 시퀀서를 사용해서 해석한 서열번호 3의 아미노산 서열을 가지는 혈청형 3 뎅기 바이러스 친주 16562와의 상동성이 95% 이상 있고, 그 친주의 209 위치, 582 위치, 1211 위치, 1563 위치의 어느 하나의 아미노산이 완전하게 돌연변이되어 있으며, 추가로 이하의 (7)∼(10)의 어느 하나 이상의 돌연변이를 가지는 약독화 혈청형 3 뎅기 바이러스;
(7) K/R671K
(8) A687V
(9) T764I/T
(10) A1237T
[22] 상기 [21]에서, 서열번호 3으로 나타내는 친주 16562의 아미노산 서열과의 상동성이 97% 이상 있는 약독화 혈청형 3 뎅기 바이러스.
[23] 상기 [21]에서, 서열번호 3으로 나타내는 친주 16562의 아미노산 서열과의 상동성이 99% 이상 있는 약독화 혈청형 3 뎅기 바이러스.
[24] 상기 [21] 내지 [23] 중 어느 하나에서, 209 위치, 582 위치, 1211 위치, 1563 위치, 및 1654 위치의 아미노산이 완전하게 돌연변이되어 있는 약독화 혈청형 3 뎅기 바이러스.
[25] 상기 [24]에서, 209 위치의 돌연변이가 I209L인 약독화 혈청형 3 뎅기 바이러스.
[26] 상기 [24]에서, 582 위치의 돌연변이가 S582G인 약독화 혈청형 3 뎅기 바이러스.
[27] 상기 [24]에서, 1211 위치의 돌연변이가 F1211L인 약독화 혈청형 3 뎅기 바이러스.
[28] 상기 [24]에서, 1563 위치의 돌연변이가 Q1563K인 약독화 혈청형 3 뎅기 바이러스.
[29] 상기 [24]에서, 209 위치의 돌연변이가 I209L이며, 582 위치의 돌연변이가 S582G이고, 1211 위치의 돌연변이가 F1211L이고, 1563 위치의 돌연변이가 Q1563K인 약독화 혈청형 3 뎅기 바이러스.
[30] 상기 [21] 내지 [29] 중 어느 하나에서, 상기 (7)∼(10)의 모든 돌연변이를 가지는 약독화 혈청형 3 뎅기 바이러스.
[31] 차세대 시퀀서를 사용해서 해석한 서열번호 4의 아미노산 서열을 가지는 혈청형 4 뎅기 바이러스 친주 1036과의 상동성이 95% 이상 있고, 그 친주에 2187 위치 또는 2354 위치의 아미노산이 완전하게 돌연변이되어 있으며, 추가로, 624 위치, 742 위치, 1628 위치, 2286 위치, 2482 위치, 또는 2508 위치에 돌연변이를 가지지 않는 약독화 혈청형 4 뎅기 바이러스.
[32] 상기 [31]에서, 서열번호 4로 나타내는 친주 1036의 아미노산 서열과의 상동성이 97% 이상 있는 약독화 혈청형 4 뎅기 바이러스.
[33] 상기 [31]에서, 서열번호 4로 나타내는 친주 1036의 아미노산 서열과의 상동성이 99% 이상 있는 약독화 혈청형 4 뎅기 바이러스.
[34] 상기 [31] 내지 [33] 중 어느 하나에서, 2187 위치, 및 2354 위치의 아미노산이 완전하게 돌연변이되어 있는 약독화 혈청형 4 뎅기 바이러스.
[35] 상기 [34]에서, 2187 위치의 돌연변이가 L2187F인 약독화 혈청형 4 뎅기 바이러스.
[36] 상기 [34]에서, 2354 위치의 돌연변이가 F/L2354S인 약독화 혈청형 4 뎅기 바이러스.
[37] 상기 [34]에서, 2187 위치의 돌연변이가 L2187F이고, 2354 위치의 돌연변이가 F/L2354S인 약독화 혈청형 4 뎅기 바이러스.
[38] 상기 [31] 내지 [37] 중 어느 하나에서, 624 위치, 742 위치, 1628 위치, 2286 위치, 2482 위치, 및 2508 위치에 돌연변이를 가지지 않는 약독화 혈청형 4 뎅기 바이러스.
[39] [1] 내지 [9] 중 어느 하나에 기재된 약독화 혈청형 1 뎅기 바이러스, [10] 내지 [20] 중 어느 하나에 기재된 약독화혈청형 2 뎅기 바이러스, [21] 내지 [30] 중 어느 하나에 기재된 약독화 혈청형 3 뎅기 바이러스, 및 [31] 내지 [38] 중 어느 하나에 기재된 약독화 혈청형 4 뎅기 바이러스로 이루어지는 그룹에서 선택되는 1개 이상의 약독화 뎅기 바이러스를 항원으로서 포함하는 약독화 뎅기 바이러스 백신.
[40] [1] 내지 [9] 중 어느 하나에 기재된 약독화 혈청형 1 뎅기 바이러스, [10] 내지 [20] 중 어느 하나에 기재된 약독화혈청형 2 뎅기 바이러스, [21] 내지 [30] 중 어느 하나에 기재된 약독화 혈청형 3 뎅기 바이러스, 및 [31] 내지 [38] 중 어느 하나에 기재된 약독화 혈청형 4 뎅기 바이러스를 항원으로 포함하는 약독화 4가 뎅기 바이러스 백신.
[41] 상기 [39] 또는 [40]에서, 혈청형 1, 2, 3 및 4의 뎅기 바이러스 항원의 혼합비율이 1:1:1:1, 5:3:5:3, 또는 5:3:3:3인 약독화 뎅기 바이러스 백신.
[42] 상기 [39] 내지 [41] 중 어느 하나에서, 혈청형 1, 2, 3 및 4의 뎅기 바이러스 각항원량이, 1∼7log10FFU/dose인 약독화 뎅기 바이러스 백신.
[43] 차세대 시퀀서를 사용해서 해석한 서열번호 1의 아미노산 서열을 가지는 혈청형 1 뎅기 바이러스 친주 03135를 초기 개 신장(PDK)세포에서 15∼25 계대해서 약독주로 하고, 상기 약독주를 추가로 아프리카 녹색원숭이 신장(Vero) 세포에서 계대하는 것을 포함하는 [1] 내지 [9]에 기재된 약독화 혈청형 1 뎅기 바이러스의 제조방법.
[44] 차세대 시퀀서를 사용해서 해석한 서열번호 1의 아미노산 서열을 가지는 혈청형 1 뎅기 바이러스 친주 03135를 PDK 세포에서 15 계대해서 약독주로 하고, 상기 약독주를 추가로 Vero 세포에서 계대하는 것을 포함하는 [1] 내지 [9]에 기재된 약독화 혈청형 1 뎅기 바이러스의 제조방법.
[45] 차세대 시퀀서를 사용해서 해석한 서열번호 2의 아미노산 서열을 가지는 혈청형 2 뎅기 바이러스 친주 99345를 PDK 세포에서 20∼35 계대해서 약독주로 하고, 상기 약독주를 추가로 Vero 세포에서 계대하는 것을 포함하는 [10] 내지 [20]에 기재된 약독화 혈청형 2 뎅기 바이러스의 제조방법.
[46] 차세대 시퀀서를 사용해서 해석한 서열번호 2의 아미노산 서열을 가지는 혈청형 2 뎅기 바이러스 친주 99345를 PDK 세포에서 25 계대해서 약독주로 하고, 상기 약독주를 추가로 Vero 세포에서 계대하는 것을 포함하는 [10] 내지 [20]에 기재된 약독화 혈청형 2 뎅기 바이러스의 제조방법.
[47] 차세대 시퀀서를 사용해서 해석한 서열번호 3의 아미노산 서열을 가지는 혈청형 3 뎅기 바이러스 친주 16562를 초기 녹색 원숭이 신장(PGMK)세포에서 20∼30 계대, PDK 세포에서 2∼5 계대해서 약독주로 하고, 상기 약독주를 추가로 Vero 세포에서 계대하는 것을 포함하는 [21] 내지 [30]에 기재된 약독화 혈청형 3 뎅기 바이러스의 제조방법.
[48] 차세대 시퀀서를 사용해서 해석한 서열번호 3의 아미노산 서열을 가지는 혈청형 3 뎅기 바이러스 친주 16562를 PGMK 세포에서 30 계대, PDK 세포에서 4 계대해서 약독주로 하고, 상기 약독주를 추가로 Vero 세포에서 계대하는 것을 포함하는 [21] 내지 [30]에 기재된 약독화 혈청형 3 뎅기 바이러스의 제조방법.
[49] 차세대 시퀀서를 사용해서 해석한 서열번호 4의 아미노산 서열을 가지는 혈청형 4 뎅기 바이러스 친주 1036을 PDK 세포에서 35∼45 계대해서 약독주로 하고, 상기 약독주를 추가로 Vero 세포에서 계대하는 것을 포함하는 [31] 내지 [38]에 기재된 약독화 혈청형 4 뎅기 바이러스의 제조방법.
[50] 차세대 시퀀서를 사용해서 해석한 서열번호 4의 아미노산 서열을 가지는 혈청형 4 뎅기 바이러스 친주 1036을 PDK 세포에서 40 계대해서 약독주로 하고, 상기 약독주를 추가로 Vero 세포에서 계대하는 것을 포함하는 [31] 내지 [38]에 기재된 약독화 혈청형 4 뎅기 바이러스의 제조방법.
본 발명은 4종의 모든 뎅기 바이러스 혈청형에 대한 중화 항체 응답을 단회 투여라해도 유도할 수 있고, 시판되고 있는 다른 약독 생바이러스 백신과 동등 레벨의 안전성(인용성)을 가지는 뎅기 백신을 제공하는 것이다.
도 1은 중화 항체가의 장기 추이(혈청형 1)를 나타낸다.
도 2는 중화 항체가의 장기 추이(혈청형 2)를 나타낸다.
도 3은 중화 항체가의 장기 추이(혈청형 3)를 나타낸다.
도 4는 중화 항체가의 장기 추이(혈청형 4)를 나타낸다.
도 5는 중화 항체가의 장기 추이와 접종 회수 비의존성(혈청형 1)을 나타낸다.
도 6은 중화 항체가의 장기 추이와 접종 회수 비의존성(혈청형 2)을 나타낸다.
도 7은 중화 항체가의 장기 추이와 접종 회수 비의존성(혈청형 3)을 나타낸다.
도 8은 중화 항체가의 장기 추이와 접종 회수 비의존성(혈청형 4)을 나타낸다.
도 9는 중화 항체 응답의 항원량 비의존성(혈청형 1)을 나타낸다.
도 10은 중화 항체 응답의 항원량 비의존성(혈청형 2)을 나타낸다.
도 11은 중화 항체 응답의 항원량 비의존성(혈청형 3)을 나타낸다.
도 12는 중화 항체 응답의 항원량 비의존성(혈청형 4)을 나타낸다.
도 13은 4가 뎅기 백신의 제작 플로우를 나타낸다.
본 발명에 의하면, 4종의 모든 뎅기 바이러스 혈청형에 대한 중화 항체 응답을 단회 투여로도 유도하는 것이 가능해서, 출시되어 있는 다른 약독 생바이러스 백신과 동등 레벨의 안전성(인용성)을 가지는 뎅기 백신이 제공된다.
이하, 본 발명이 호적한 실시형태에 대해서 상세하게 설명한다. 단, 본 발명은 이하의 실시형태에 한정되는 것은 아니다.
본 발명은 임상 분리된 야생형 뎅기 바이러스(친주)를 PDK 세포, 또는 PGMK 세포와 PDK 세포에서 계대하는 것에 의해서 수득된 약독주를, 추가로 Vero 세포에서 계대해서 수득되는 약독화 뎅기 바이러스에 관한 것으로, 뎅기 백신용으로 사용되는 시드주(VMS(Virus Master Seed), VWS(Virus Working Seed) 등)이나 제품 상당 계대주(GLP 시험용 벌크(Pre-CTM: Pre-Clinical Trial Material) 등), 및 그것들을 사용해서 제작되는 뎅기 백신이 포함된다. 본 발명에서는 친주, 약독주, 시드주, 제품 상당 계대주의 아미노산 서열을 비교 검토하는 것에　의해, 친주와 약독주를 식별할 수 있는 부위 및 약독주와 시드주, 제품 상당 계대주를 식별할 수 있는 부위를 특정하고 있다.
본 발명의 약독화 혈청형 1 뎅기 바이러스는 임상 분리주인 친주 03135(서열번호1)를 초기 개 신장(PDK) 세포에서 15∼25 계대해서 제작한 약독주 1을, 추가로 Vero 세포에서 계대하는 것에 의해 수득된다. 약독주 1은 계대에 따라서, 친주 03135에 대해서, 483 위치의 글루탐산, 568 위치의 리신, 1663 위치의 아스파라긴 중 어느 하나 또는 전부가 완전히 다른 아미노산으로 돌연변이되어 있으며, 바람직하게는 E483K, K568R, N1663K의 돌연변이를 가지는 본 발명의 약독화 혈청형 1 뎅기 바이러스는, 추가로 Vero 세포에서 계대하는 것에　의해, 상기 돌연변이에 부가해서, 482 위치, 484 위치, 2353 위치, 2364 위치의 어느 하나 이상에도 돌연변이를 가지고 있고, 약독주 1과 유전적으로 식별가능하다.
본 발명의 약독화 혈청형 2 뎅기 바이러스는 임상 분리주인 친주 99345(서열번호 2)를, PDK 세포에서 20∼35 계대해서 제작된 약독주 2를, 추가로 Vero 세포에서 계대하는 것에 의해 수득된다. 약독주 2는 계대에 따라서 친주 99345에 대해서, 143 위치의 아스파라긴산, 400 위치의 트레오닌, 1102 위치의 아스파라긴산, 1308 위치의 로이신, 1654 위치의 글루탐산 중 어느 하나 또는 전부가 완전히 다른 아미노산으로 돌연변이되어 있고, 바람직하게는 D143N, T400K, D1102N, L1308F, E1654K의 돌연변이를 갖는다. 본 발명의 약독화 혈청형 2 뎅기 바이러스는, 추가로 Vero 세포에서 계대하는 것에　의해, 2347 위치 또는 2828 위치에도 돌연변이를 가지고 있고, 약독주 2와 유전적으로 식별가능하다.
본 발명의 약독화 혈청형 3 뎅기 바이러스는 임상 분리주인 친주 16562(서열번호3)를, PGMK 세포에서 20∼30 계대하고, 추가로 PDK 세포에서 2∼5 계대해서 제작된 약독주 3을, 추가로 Vero 세포에서 계대하는 것에 의해 수득된다. 약독주 3은 친주 16562에 대해서, 209 위치의 이소로이신, 582 위치의 세린, 1211 위치의 페닐알라닌, 1563 위치의 글루타민 중 어느 하나 또는 전부가 완전히 다른 아미노산으로 돌연변이되어 있고, 바람직하게는, I209L, S582G, F1211L, Q1563K의 돌연변이를 갖는다. 본 발명의 약독화 혈청형 3 뎅기 바이러스는 추가로 Vero 세포에서 계대하는 것에　의해, 671 위치, 687 위치, 764 위치, 1237 위치의 어느 하나 이상에도 돌연변이를 가지고 있고, 약독주 3과 유전적으로 식별가능하다.
본 발명의 약독화 혈청형 4 뎅기 바이러스는 임상 분리주인 친주 1036(서열번호4)을, PDK 세포에서 35∼45 계대해서 제작된 약독주 4를, 추가로 Vero 세포에서 계대하는 것에 의해 수득된다. 약독주 4는 친주 1036에 대해서, 2187 위치의 로이신, 2354 위치의 페닐알라닌/로이신(혼합 아미노산)의 어느 한쪽 또는 양쪽이 완전히 다른 아미노산으로 돌연변이되어 있고, 바람직하게는, L2187F, F/L2354S의 돌연변이를 갖는다. 본 발명의 약독화 혈청형 4 뎅기 바이러스는 624 위치, 742 위치, 1628 위치, 2286 위치, 2482 위치, 또는 2508 위치에 돌연변이를 가지지 않고, 약독주 4과 유전적으로 식 별가능하다.
본 발명에는 상기의 각 혈청형의 뎅기 바이러스를 항원으로 포함하는 백신도 포함된다. 혈청형 1∼4 각각의 단가 백신일 수도 있고, 어느 하나의 혈청형의 조합일 수도 있고, 4가 백신일 수도 있다. 4가 백신의 경우, 혈청형 1, 2, 3, 및 4의 항원혼합비율은 임의일 수 있지만, 특히 1:1:1:1, 5:3:5:3, 5:3:3:3이 바람직하다. 본 발명의 뎅기 바이러스 백신을 프라이밍 백신으로 하고, 다른 백신을 부스터로서 조합시킬 수도 있다. 또, 다른 플라비바이러스 백신과 혼합할 수도 있고, 다른 감염증 등의 백신과 혼합해서 사용할 수도 있다.
백신의 제형은 적시 선택할 수 있다. 액상 제제일 수도 있고, 분말 제제일 수도 있고, 동결 건조 제제일 수도 있다.
친주 16562와 친주 1036은 특허문헌 1에 개시되고 있고, 또, 프랑스의 CNCM(Collection Nationalede Culturesde Micro-organismes)에, 각각 I-2482, I-2483로 기탁되어 있다. 또, 친주 03135는 GenBank에 ADJ18295.1로 부분 서열이 등록되어 있고, 친주 99345는 GenBank에 ADJ18294.1로서 부분 서열이 등록되어 있다. 본 발명의 약독화 뎅기 바이러스(혈청형 1∼4)는 미국의 ATCC(American Type Culture Collection; 10801 University Boulevard, Manassas, VA 20110 USA)에 각각 수탁번호 PTA-123506, PTA-123505, PTA-123507, PTA-123508로 기탁되어 있다.
본 발명에서는 뎅기 바이러스의 염기서열을 차세대 시퀀서를 사용해서 해석하고 있다. 차세대 시퀀서란 제2 세대 시퀀서라고도 하며, 예를 들면 Illumina사의 SBS시퀀서의 경우, 수백만 개 이상의 DNA 단편의 염기서열을 동시 병행적으로 결정할 수 있다. 원리는 기본적으로는 모세관 전기영동을 사용한 종래의 생거법과 유사하고, DNA 단편을 템플레이트로 해서 1염기씩 재합성할 때의 형광강도를 검출해서 염기서열을 결정하는 것이다. 생거법에서는 1∼96개의 DNA 단편을 동시처리하는 것에 대해, 차세대 시퀀서에서는 생거법에서의 모세관에 상당하는 「클러스터」를 형성시킴으로써, 수백만 개 이상의 DNA 단편에 대해서 대량 병렬처리를 실시하는 것에 차이가 있다. 이것에 의해 시퀀스 해석의 스피드는 비약적으로 향상하고, 커다란 게놈 영역을 대상으로 삼는 연구나 높은 리드 수가 요구되는 저빈도 돌연변이의 연구나 아집단(quasispecies)의 연구가 가능하게 되었다.
차세대 시퀀서를 사용한 염기서열 해석에 의해, 바이러스의 아집단의 연구가 가능하다. 혼합 염기가 있는 경우에 그 혼합비를 얻을 수 있고, 아집단을 확인할 수 있는 것이 큰 특징이다. 본 명세서에서는 그 염기서열을 아미노산 서열로 변환해서 표기하고 있으며, 예를 들면, 약독주의 제484 부위는 리신이 30%, 아르기닌이 70% 등과 같이 나타난다.
본 명세서에서는 친주나 약독주 등과의 비교에 있어서 돌연변이가 있는 부위는 K482E, K482E/K 등과 같이 기재한다. 여기에서, 「K482E」란 482 위치의 리신이 글루탐산으로 돌연변이되어 있는 것을 의미한다. 「K482E/K」란 리신이었던 482 위치에, 돌연변이의 결과, 글루탐산으로 돌연변이한 것과 리신으로 돌연변이한 것의 양쪽이 혼재하고 있는 것을 의미한다.
뎅기 바이러스 게놈은 C단백질, M단백질, E단백질과 같은 구조 단백질, 그리고 바이러스 입자를 구성하지 않고 복제 시에 작용하하는 7개의 비구조(NS) 단백질을 코딩하고 있다. 뎅기 바이러스 게놈으로부터는 1개의 폴리 단백질이 번역된 후, 각각의 구조 단백질 및 비구조 단백질로 프로세싱을 받는다. 본 명세서에서는 아미노산 서열 중의 위치를 계산하는데 있어서, 폴리 단백질의 개시 코돈을 기점으로 하고 있다.
차세대 시퀀서로 측정할 때는, 우선 뎅기 바이러스로부터 RNA를 추출하고, Random 프라이머에 의한 역전사, 더블-스트랜드화, 어댑터 서열부가, 증폭을 거치고, SBS 시퀀서로 단쇄 서열의 집합 데이터를 얻는다. 친주의 데이터로부터 어셈블러 Velvet에 의해 콘틱을 작성하고, 뎅기 바이러스에 상동에서 가장 긴 콘틱을 얻는다. 당해 콘틱과 상동성이 높은 유전자 뱅크 상의 서열을 얼라인먼트 하고, 5' 말단 및/또는 3' 말단의 서열을 보충해서 리시퀀싱 해석의 참조 서열로 한다. 리시퀀싱 해석에서는, 맵퍼 BWA를 사용해서 시퀀스 데이터를 참조 서열로 매핑하고, 바이러스 게놈 서열을 혼합 염기도 가미해서 결정한다. 수득된 바이러스 게놈 서열로부터 혼합 아미노산을 가미해서 아미노산 서열을 얻는다. 또, 혼합 아미노산에는 혼합비로서 전체에 10% 이상 존재하는 아미노산만을 포함하는 것으로 한다.
차세대 시퀀서의 기종은 MiSeq이나 HiSeq(모두 Illumina사) 등이 알려져 있지만, 다른 기종이더라도 상관없다.
뎅기 바이러스의 약독 특성은 플라크 사이즈 어세이나 증식 온도 감수성 시험, 동물 실험 등에 의해 확인할 수 있지만, 이것에 한정되지 않는다. 플라크 사이즈 어세이에서는 예를 들면 붉은털 원숭이 유래의 LLC-MK2 세포를 사용하고, 플라크의 직경을 측정하고, 친주와 비교해서 직경이 작으면 약독 특성이 확인된다. 뎅기 바이러스의 증식 온도 감수성 시험에서는 예를 들면 Vero 세포를 사용하고, 배양 온도를 변화시키고, 친주와 비교해서 고온 증식능이 감소하고 있으면 약독 특성이 확인된다.
뎅기 바이러스의 약효ㆍ안전성 시험은 NHP(Non-Human Primate)이나 일부의 유전자 재조합 면역 결손 마우스를 사용해서 수행된다. NHP의 대표예는 사이노몰거스 원숭이다.
중화 항체가 측정은 플라크를 지표로 하는 PRNT(Plaque Reduction Neutralizing Test)법이나 바이러스 유래 단백질을 항체에서 표지하는 Immunospot PRNT 또는 FRNT(Focus Reduction Neutralization Titer)법을 사용할 수 있다.
혈중 바이러스량의 측정은 직접적으로 플라크 사이즈 어세이를 사용거나, 간접적으로 바이러스 게놈 단편을 검출하는 RT-qPCR(Real Time Quantitative Polymerase Chain Reaction)법이 주로 사용되지만, 이것에 한정되지 않는다.
이하, 실시예에 의해 본 발명을 상세하게 설명하지만, 본 발명은 이것들의 실시예에 전혀 한정되는 것은 아니다.
실시예 1
(1) 약독주의 제작
태국 Mahidol 대학에서, 신규 백신 후보가 되는 약독화 뎅기 바이러스(혈청형 1, 혈청형 2, 혈청형 3 및 혈청형 4)가 다음 순서로 제작되었다. 혈청형 1의 약독화 뎅기 바이러스는 임상 분리주인 03135를 친주로 해서, 초기 개 신장(PDK) 세포에서 15 계대하는 것으로 제작되었다. 혈청형 2의 약독화 뎅기 바이러스는 임상 분리주인 99345를 친주로 해서, PDK 세포에서 25 계대하는 것으로 제작되었다. 혈청형 3의 약독화 뎅기 바이러스는 임상 분리주인 16562를 친주로 해서, 초대 녹색원숭이 신장(PGMK) 세포에서 30 계대하고, 추가로 PDK 세포에서 4 계대하는 것으로 제작되었다. 혈청형 4의 약독화 뎅기 바이러스는 임상 분리주인 1036을 친주로 해서, PDK 세포에서 40 계대하는 것으로 제작되었다. 또, 혈청형 1의 약독화 뎅기 바이러스는 Vero 세포에서 추가로 3 계대되었다. 이것들의 약독화 뎅기 바이러스를 각각 약독주 1∼4라고 칭한다.
(2) PreVMS의 제작
VMS(Virus Master Seed)의 제작에 즈음해서, 우선, preVMS 시드를 제작했다. preVMS 시드는 약독주 1∼4를 Vero 세포에서 각각 2 계대, 3 계대, 3 계대, 2 계대하는 것으로 얻었다. 이하에 각 혈청형의 PreVMS 시드의 구체적인 제작 방법에 대해서 기재한다.
(i) 혈청형 1: PreVMS 시드(PreVMS1)
1계대째는 Multiplicity of Infection(MOI) 0.001로 약독주 1을 접종하고, 37℃ㆍ5% CO2 하에서 90분 동안 바이러스를 Vero 세포에 흡착시켰다. 그 다음에, 무혈청 배지를 첨가하고, 37℃ㆍ5% CO2 하에서 배양하고, 배양 12일 후에 배양 용기마다 2회 동결 융해했다. 그것으로부터, 원심분리(1500rpmㆍ5분 동안)에서 세포 및 세포 잔재를 침전시키고, 상청액을 회수했다. 안정제로서 상청액에 20% 양의 FBS(Fetal Bovine Serum; 소 태아 혈청)을 첨가 후, 탄산수소나트륨에 의해 pH를 조정하고, -60℃ 이하에서 보존했다. 플라크 어세이로 측정한 결과, 감염가는 1.6×107 PFU/㎖이었다.
2 계대째는, 약독주 1을 1 계대한 바이러스를 MOI 0.001로 접종하고, 분간 90분 동안 바이러스를 Vero 세포에 흡착시켰다. 그 다음에, 무혈청 배지를 첨가하고, 37℃ㆍ5% CO2 하에서 배양하고, 배양 9일 후에 배양 상청을 회수했다. 배양 상청에 안정제로서 20% 최종 농도가 되도록 FBS를 첨가 후, 탄산수소나트륨에 의해 pH를 조정하고, 일시적으로 동결보존했다. 융해 후, 0.2㎛ 무균 여과하고, -60℃ 이하에서 보존했다. 플라크 어세이로 측정한 결과, PreVMS1의 감염가는 5.1×106 PFU/㎖이었다.
(ii) 혈청형 2: PreVMS 시드(PreVMS2)
1계대째는 MOI 0.001로 약독주 2를 접종하고, 37℃ㆍ5% CO2 하에서 90분 동안 바이러스를 Vero 세포에 흡착시켰다. 그 다음에 MEM+2% 배지를 첨가하고, 37℃ㆍ5% CO2 하에서 배양하고, 배양 10일 후에 배양 용기마다 2회 동결 융해했다. 그것으로부터, 원심분리(1500rpmㆍ5분 동안)에 의해 세포 및 세포 잔재를 침전시키고, 상청액을 회수했다. 안정제로서 20% 최종 농도가 되도록 FBS를 첨가 후, 탄산수소나트륨에 의해 pH를 조정하고, 0.2㎛ 무균 여과하고, -60℃ 이하에서 보존했다. 플라크 어세이로 측정한 결과, 감염가는 2.7×105 PFU/㎖이었다.
2 계대째는 약독주 2를 1 계대한 바이러스를 MOI 0.001로 접종하고, 37℃ㆍ5% CO2 하에서 90분 동안 바이러스를 Vero 세포에 흡착시켰다. 그 다음에, MEM+2% 배지를 첨가하고, 37℃ㆍ5% CO2 하에서 배양하고, 배양 11일 후에 배양 용기마다 2회 동결 융해 했다. 그것으로부터, 원심분리(1500rpmㆍ5분 동안)에 의해 세포 및 세포 잔재를 침전시키고, 상청액을 회수했다. 0.2㎛ 무균 여과 후, 안정제로서 상청액에 20% 양의 FBS를 첨가하고, 탄산수소나트륨에 의해 pH를 조정한 후, -60℃ 이하에서 보존했다. 플라크 어세이로 측정한 결과 감염가는 1.1×104 PFU/㎖이었다.
3 계대째는 약독주 2를 2 계대한 바이러스를 MOI 0.001로 접종하고, 37℃에서 90분 동안 바이러스를 Vero 세포에 흡착시켰다. 그 다음에, MEM+2% 배지를 첨가하고, 37℃에서 기밀 마개 배양하고, 배양 6일 후에 배양 상청을 회수했다. 그것으로부터, 원심분리(1500rpmㆍ5분 동안)에 의해 세포 및 세포 잔재를 침전시키고, 상청액을 회수했다. 0.2㎛ 무균 여과 후, 안정제로서 상청액에 20% 양의 FBS를 첨가하고, 탄산수소나트륨에 의해 pH를 조정한 후, -60℃ 이하에서 보존했다. 플라크 어세이로 측정한 결과, PreVMS2의 감염가는 7.1×104 PFU/㎖이었다.
(iii) 혈청형 3: PreVMS 시드(PreVMS3)
1계대째는 MOI 0.001로 약독주 3을 접종하고, 37℃ㆍ5% CO2 하에서 90분 동안 바이러스를 Vero 세포에 흡착시켰다. 그 다음에, 무혈청 배지를 첨가하고, 37℃ㆍ5% CO2 하에서 배양하고, 배양 10일 후에 배양 상청을 회수했다. 0.2㎛ 무균 여과 후, 안정제로서 배양 상청에 20% 양의 FBS를 첨가하고, 탄산수소나트륨에 의해 pH를 조정한 후, -60℃ 이하에서 보존했다. 플라크 어세이로 측정한 결과, 감염가는 9.8×104 PFU/㎖이었다.
2 계대째는 약독주 3을 1 계대한 바이러스를 MOI 0.001로 접종하고, 37℃ㆍ5% CO2 하에서 90분 동안 바이러스를 Vero 세포에 흡착시켰다. 그 다음에, 무혈청 배지를 첨가하고, 37℃ㆍ5% CO2 하에서 배양하고, 배양 7일 후에 배양 상청을 회수했다. 그것으로부터, 원심분리(1500rpmㆍ5분 동안)에 의해 세포 및 세포 잔재를 침전시키고, 상청액을 회수했다. 0.2㎛ 무균 여과 후, 안정제로서 상청액에 20% 양의 FBS를 첨가하고, 탄산수소나트륨에 의해 pH를 조정한 후, -60℃ 이하에서 보존했다. 플라크 어세이로 측정한 결과, 감염가는 1.3×105 PFU/㎖이었다.
3 계대째는 약독주 3을 2 계대한 바이러스를 MOI 0.001로 접종하고, 37℃ㆍ5% CO2 하에서 90분 동안 바이러스를 Vero 세포에 흡착시켰다. 그 다음에, 무혈청 배지를 첨가하고, 37℃ㆍ5% CO2 하에서 배양하고, 배양 7일 후에 배양 상청을 회수했다. 그것으로부터, 원심분리(1500rpmㆍ5분 동안)에 의해 세포 및 세포 잔재를 침전시키고, 상청액을 회수했다. 0.2㎛ 무균 여과 후, 안정제로서 상청액에 20% 량의 FBS를 첨가후, 탄산수소나트륨에 의해 pH를 조정하고, -60℃ 이하에서 보존했다. 플라크 어세이로 측정한 결과, PreVMS3의 감염가는 3.7×105 PFU/㎖이었다.
(iv) 혈청형 4: PreVMS시드(PreVMS4)
1계대째는 MOI 0.001로 약독주 4를 접종하고, 37℃ㆍ5% CO2 하에서 90분 동안 바이러스를 Vero 세포에 흡착시켰다. 그 다음에, 무혈청 배지를 첨가하고, 37℃ㆍ5% CO2 하에서 배양하고, 배양 7일 후에 배양 상청을 회수했다. 그것으로부터, 원심분리(1500rpmㆍ5분 동안)에 의해 세포 및 세포 잔재를 침전시키고, 상청액을 회수했다. 0.2㎛ 무균 여과 후, 안정제로서 20% 최종 농도가 되도록 FBS를 첨가하고, 탄산수소나트륨에 의해 pH를 조정한 후, -60℃ 이하에서 보존했다. 플라크 어세이로 측정한 결과, 감염가는 1.9×107 PFU/㎖이었다.
2 계대째는 약독주 4를 1 계대한 바이러스를 MOI 0.001로 접종하고, 37℃ㆍ5% CO2 하에서 90분 동안 바이러스를 Vero 세포에 흡착시켰다. 그 다음에, 무혈청 배지를 첨가하고, 37℃ㆍ5% CO2 하에서 배양하고, 배양 5일 후에 배양 상청을 회수했다. 그것으로부터, 원심분리(2000rpmㆍ5분 동안)에 의해 세포 및 세포 잔재를 침전시키고, 상청액을 회수했다. 0.2㎛ 무균 여과 후, 안정제로서 20% 최종 농도가 되도록 FBS를 첨가후, 탄산수소나트륨에 의해 pH를 조정하고, -60℃ 이하에서 보존했다. 플라크 어세이로 측정한 결과, PreVMS4의 감염가는 5.5×107 PFU/㎖이었다.
(3) VMS의 제작
PreVMS를 추가로 1 계대하고, 바이러스 뱅크의 최상류가 되는 VMS1, VMS2, VMS3 및 VMS4을 제작했다. 이하에 각 혈청형의 VMS가 구체적인 제작 방법에 대해서 기재한다.
(i) VMS1
MOI 0.01로 PreVMS1을 접종하고, 37℃ㆍ5% CO2 하에서 90분 동안 바이러스를 Vero 세포에 흡착시켰다. 그 다음에, 무혈청 배지를 첨가하고, 37℃ㆍ5% CO2 하에서 배양하고, 배양 7일 후에 배양 용기마다 2회 동결 융해했다. 그것으로부터, 원심분리(3000rpmㆍ10분 동안)에 의해 세포 및 세포 잔재를 침전시키고, 상청액을 회수했다. 안정제로서 20% 최종 농도가 되도록 FBS를 첨가 및 0.2% 최종 농도가 되도록 탄산수소나트륨을 첨가하고, 0.2㎛ 무균 여과 후, -60℃ 이하에서 보존했다. 플라크 어세이로 측정한 결과, VMS1의 감염가는 1.49×106 PFU/㎖이었다.
(ii) VMS2
MOI 0.001로 PreVMS2를 접종하고, 37℃ㆍ5% CO2 하에서 90분 동안 바이러스를 Vero 세포에 흡착시켰다. 그 다음에, 무혈청 배지를 첨가하고, 37℃ㆍ5% CO2 하에서 배양하고, 배양 8일 후에 배양 상청을 회수했다. 그것으로부터, 원심분리(1800rpmㆍ10분 동안)에서 세포 및 세포 잔재를 침전시키고, 상청액을 회수하고, 0.2㎛ 무균 여과 했다. 안정제로서 상청액에 0.258배량의 FBS 및 0.03445배량의 탄산수소나트륨을 첨가하고, -60℃ 이하에서 보존했다. 플라크 어세이로 측정한 결과, VMS2의 감염가는 2.07×105 PFU/㎖이었다.
(iii) VMS3
MOI 0.01로 PreVMS3을 접종하고, 37℃ㆍ5% CO2 하에서 90분 동안 바이러스를 Vero 세포에 흡착시켰다. 그 다음에, 무혈청 배지를 첨가하고, 37℃ㆍ5% CO2 하에서 배양하고, 배양 6일 후에 배양 상청을 회수했다. 그것으로부터, 원심분리(3000rpmㆍ10분 동안)에 의해 세포 및 세포 잔재를 침전시키고, 상청액을 회수했다. 안정제로서 20% 최종 농도가 되도록 FBS를 첨가 및 0.2% 최종 농도가 되도록 탄산수소나트륨을 첨가하고, 0.2㎛ 무균 여과 후, -60℃ 이하에서 보존했다. 플라크 어세이로 측정한 결과, VMS3의 감염가는 6.49×104 PFU/㎖이었다.
(iv) VMS4
MOI 0.01로 PreVMS4를 접종하고, 37℃ㆍ5% CO2 하에서 90분 동안 바이러스를 Vero 세포에 흡착시켰다. 그 다음에, 무혈청 배지를 첨가하고, 37℃ㆍ5% CO2 하에서 배양하고, 배양 6일 후에 배양 상청을 회수했다. 그것으로부터, 원심분리(3000rpmㆍ10분 동안)에 의해 세포 및 세포 잔재를 침전시키고, 상청액을 회수했다. 안정제로서 20% 최종 농도가 되도록 FBS를 첨가 및 0.2% 최종 농도가 되도록 탄산수소나트륨을 첨가하고, 0.2㎛ 무균 여과 후, -60℃ 이하에서 보존했다. 플라크 어세이로 측정한 결과, VMS4의 감염가는 9.39×106 PFU/㎖이었다.
(4) Pre-CTM의 제작
계속해서, GLP시험용 벌크(이하, 「Pre-CTM」; Pre-Clinical Trial Material)를 제작했다. 혈청형 1은 VMS1을 Vero 세포에서 4 계대함으로써, Pre-CTM1을 얻었다. 혈청형 2∼4는 VMS2∼4를 각각 Vero 세포에서 2 계대하고, Virus Working Seed(VWS)를 제작했다. 추가로 VWS2∼4를 각각 Vero 세포에서 2 계대(VMS로부터 계산해서 합계 4 계대)하고, Pre-CTM2∼4를 얻었다. VMS로부터 3 계대째까지는 배양 상청을 원심분리 후, 그 상청액에 안정제로서 20% 최종 농도가 되도록 FBS를 첨가하고, 0.2% 최종 농도가 되도록 탄산수소나트륨을 첨가했다. 0.2㎛ 무균 여과 후, -60℃ 이하에서 보존했다. 최종 계대가 되는 4 계대 시는 배양 상청에 0.2% 최종 농도가 되도록 탄산수소나트륨을 첨가하고, 청징 여과 및 0.2㎛ 무균 여과의 뒤, 중공사 막을 사용해서 농축했다. 농축액을 0.2㎛무균 여과한 후, -60℃ 이하에서 보존했다. 또, 본 실시예에서는 VWS1을 제작하지 않고 Pre-CTM1을 제작하고 있지만, VMS1을 Vero 세포에서 2 계대해서 VWS1을 제작한 후, 추가로 Vero 세포에서 2 계대해서 Pre-CTM1로 할 수도 있다.
(5) 서열 결정
바이러스 게놈 서열을 하기의 방법으로 조사했다. 각 혈청형의 친주, 약독주, VMS, Pre-CTM의 각각으로부터 RNA를 추출하고, Random 프라이머에 의한 역전사, 더블-스트랜드화, 어댑터 서열 부가, 증폭을 거치고, Illumina사의 SBS 시퀀서로 단쇄 서열의 집합 데이터를 얻었다. 친주의 데이터로부터 어셈블러 Velvet에 의해 콘틱을 작성하고, 뎅기 바이러스에 상동에서 가장 긴 콘틱을 얻었다. 당해 콘틱과 상동성이 높은 유전자 뱅크 상의 서열을 얼라인먼트 하고, 5' 말단 및/또는 3' 말단의 서열을 보충해서 리시퀀싱 해석의 참조 서열로 했다. 리시퀀싱 해석에서는 맵퍼 BWA를 사용해서 시퀀스 데이터를 참조 서열에 매핑하고, 바이러스 게놈 서열을 혼합 염기도 가미해서 결정했다(서열번호 17∼20에 친주 1∼4, 서열번호 21∼24에 약독주 1∼4, 서열번호 25∼28에 VMS1∼4, 서열번호 29∼32에 Pre-CTM1∼4의 염기서열을 각각 나타낸다). 수득한 바이러스 게놈 서열로부터 혼합 아미노산을 가미해서 아미노산 서열을 얻었다(서열번호 1∼4에 친주 1∼4, 서열번호 5∼8에 약독주 1∼4, 서열번호 9∼12에 VMS1∼4, 서열번호 13∼16에 Pre-CTM1∼4의 아미노산 서열을 각각 나타낸다). 또, 혼합 아미노산을 표기하는 경우에는, 혼합비로서 전체에 10% 이상 존재하는 아미노산만을 표기했다. 또, 아미노산 서열을 비교할 때는, 각 부위의 각 아미노산을 비교할 때에 그 각 혼합비가 10% 이상 괴리하고 있는 것을 임계값으로 하고, 차이의 유무를 판정했다. 예를 들면, 친주에 50 위치가 「알라닌 95%, 글리신 5%」, 약독주에 50 위치는 「알라닌 85%, 글리신 14%, 프롤린 1%」로 하는 해석결과를 얻었을 경우, 친주의 아미노산 표기는 A(알라닌만), 약독주의 아미노산 표기는 A/G(알라닌과 글리신의 혼합 아미노산)이 된다. 이 부위에 있어서, 친주와 약독주의 알라닌 혼합비를 비교하면 10% 이상 (10%)의 괴리가 있기 때문에, 친주와 약독주에서 「차이 있음」의 판정이 된다. 글리신에 대해서는 9%의 차이밖에 인정되지 않았지만, 최저 1종류의 아미노산에서 차이가 인정되면 「차이 있음」의 판정으로 하고 있다. 한편, 친주에 100위치가 「알라닌 94%, 글리신 6%」, 약독주에 100 위치는 「알라닌 85%, 글리신 15%」라고 하는 경우에도, 친주의 아미노산 표기는 A, 약독주의 아미노산 표기는 A/G가 된다. 그러나, 이 부위의 알라닌과 글리신의 혼합비를 각각 비교하면, 함께 10% 미만(9%)이기 때문에 「차이 없음」의 판정이 된다.
(6) 아미노산 서열 비교
친주와 약독주의 서열을 비교하고, 아미노산의 완전 돌연변이가 발생하고 있는 부위를 확인했다(표 1∼4). 또, 혼합 아미노산이 존재하는 친주의 부위에 대해서는 약독주의 동일 부위에 있어서 당해 친주의 혼합 아미노산에 포함되지 않는 다른 아미노산으로 돌연변이되어 있을 경우에 완전 돌연변이되어 있는 것으로 간주했다.
Figure 112018061634250-pct00001
Figure 112018061634250-pct00002
Figure 112018061634250-pct00003
Figure 112018061634250-pct00004
다음에, 약독주, VMS, Pre-CTM의 서열을 비교하고, 약독주에 대해서 VMS 및 Pre-CTM을 식별할 수 있는 부위를 확인했다(표 5∼8).
Figure 112018061634250-pct00005
Figure 112018061634250-pct00006
Figure 112018061634250-pct00007
Figure 112018061634250-pct00008
이것에 의해, 혈청형 1의 VMS 및 Pre-CTM은 친주 03135에 대해서 483 위치의 글루탐산이 리신으로, 568 위치의 리신이 아르기닌으로, 1663 위치의 아스파라긴이 리신으로 완전 돌연변이되어 있고, 이것들의 부위에서 약독주와 같은 돌연변이인 것이 확인되었다. 또, 482 위치의 리신이 글루탐산/리신의 혼합 아미노산으로, 484 위치의 리신이 아르기닌/리신의 혼합 아미노산으로, 2353 위치의 이소로이신/트레오닌의 혼합 아미노산이 트레오닌으로, 2364 위치의 알라닌이 트레오닌/알라닌의 혼합 아미노산으로 돌연변이되어 있고, 이것들의 부위에서 약독주와의 차이가 확인되었다.
혈청형 2의 VMS 및 Pre-CTM은 친주 99345에 대해서 143 위치의 아스파라긴산이 아스파라긴으로, 400 위치의 트레오닌이 리신으로, 1102 위치의 아스파라긴산이 아스파라긴으로, 1308 위치의 로이신이 페닐알라닌으로, 1654 위치의 글루탐산이 리신으로 완전 돌연변이되어 있고, 이것들의 부위에서 약독주와 동일한 돌연변이인 것이 확인되었다. 또 2347 위치의 프롤린이 프롤린/로이신의 혼합 아미노산우로, 2828 위치의 트레오닌으로, 트레오닌/메티오닌의 혼합 아미노산으로 돌연변이되어 있고, 이것들의 부위에서 약독주와의 차이가 확인되었다.
혈청형 3의 VMS 및 Pre-CTM은 친주 16562에 대해서 209 위치의 이소로이신이 로이신으로, 582 위치의 세린이 글리신으로, 1211 위치의 페닐알라닌이 로이신으로, 1563 위치의 글루타민이 리신으로 완전 돌연변이되어 있고, 이것들의 부위에서 약독주와 동일한 돌연변이인 것이 확인되었다. 또, 671 위치의 리신/아르기닌의 혼합 아미노산이 리신으로, 687 위치의 알라닌이 발린으로, 764 위치의 트레오닌이 이소로이신/트레오닌의 혼합 아미노산으로, 1237 위치의 알라닌이 트레오닌으로 돌연변이되어 있고, 이것들의 부위에서 약독주와의 차이가 확인되었다.
혈청형 4의 VMS 및 Pre-CTM은 친주 1036에 대해서 2187 위치의 로이신이 페닐알라닌으로, 2354 위치의 페닐알라닌/로이신의 혼합 아미노산이 세린으로 완전 돌연변이되어 있고, 이것들의 부위에서 약독주와 동일한 돌연변이인 것이 확인되었다. 또 624 위치, 742 위치, 1628 위치, 2286 위치, 2482 위치, 2508 위치가 돌연변이되어 있지 않고, 이것들의 부위에서 약독주와의 차이가 확인되었다.
(7) VMS의 시험관 내 약독 특성
VMS의 시험관 내 약독 특성을 확인하기 위해서, LLC-MK2 세포를 사용한 플라크 사이즈 어세이를 실시했다. 플라크의 직경을 측정하고, 측정결과에 의해 표 9과 같이 구분했다.
Figure 112018061634250-pct00009
그 결과, VMS1, VMS2, VMS3 및 VMS4의 플라크 사이즈의 분포는 각각의 친주에 대해서 작고, 약독 특성을 나타내는 것을 확인했다(표 10).
Figure 112018061634250-pct00010
(8) VMS의 증식 온도 감수성 시험
VMS1, VMS2 및 VMS4에 대해서는 35℃ 및 39℃, VMS3에 대해서는 35℃ 및 38℃에서, 각각 일정 일수 동안 Vero 세포에서 배양하고, 저온배양 시와 고온 배양 시에서의 증식량의 차이를 확인했다. 그 결과, 각각의 친주와 비교하고, VMS1, VMS2, VMS3 및 VMS4 전부에 있어서 증식 온도 감수성이 높고, 약독특 성을 나타내는 것을 확인했다(표 11).
Figure 112018061634250-pct00011
실시예 2
1. 사이노몰거스 원숭이를 사용한 4가 뎅기 바이러스 제제의 약효ㆍ안전 평가시험 1
뎅기 바이러스 감수성 실험동물은 NHP(Non-Human Primate)나 일부의 유전자 재조합 면역 결손 마우스에 한정되기 때문에, NHP인 사이노몰거스 원숭이를 사용하고, 약효(중화 항체 응답) 및 안전성(바이러스 혈증)의 평가를 실시했다. 또, 중화 항체 응답은 백신개발 상의 주요 평가항목에 하나이고, 바이러스 혈증은 인간에서의 병원성 및 증상의 중독화에 기인하는 요소의 하나이다.
(1) 4가 뎅기 바이러스 제제(5555 제제)의 조제
실제의 백신 투여 시에 가까운 반응을 관찰하기 위해서, VMS1, VMS2, VMS3 및 VMS4를 Vero 세포에서 4 계대한 후(VMSVP1, VMSVP2, VMSVP3, VMSVP4), 4가 뎅기 바이러스 제제를 조제했다. 그 때, 각 VMSVP의 투여량이 5log10PFU/㎖, 1.0mL/dose가 되도록 조제했다(5555 제제). 이 5555 제제를 3마리의 사이노몰거스 원숭이의 전완부 피하에 마취 하에서 투여했다(5555그룹). 투여 시를 Day 0으로 하고, Day 0, 1, 2, 3, 4, 6, 8, 10, 14, 30, 60에 마취 하에서 채혈을 실시했다.
(2) Immunospot PRNT법에 의한 중화 항체가 측정
Immunospot PRNT(Plaque Reduction Neutralizing Test)법에 의해, Day 0, 14, 30, 60의 채혈에서 얻은 혈청 중의 중화 항체가를 측정했다. 그 결과, 전 3개체, 4종의 모든 혈청형에 대해서 Day 14이후에 중화 항체가 양전(positive conversion)하고 있고, 적어도 Day 60까지의 사이는 양성을 유지하고 있었다(양전 기준은 10PRNT50 이상; 표 12 및 표 13).
Figure 112018061634250-pct00012
Figure 112018061634250-pct00013
(3) RT-qPCR법에 의한 혈중 게놈량의 측정
RT-qPCR(Real Time Quantitative Polymerase Chain Reaction)법에 의해, Day 1, 2, 3, 4, 6, 8, 10, 14에 채취한 혈청 중의 바이러스 게놈량을 측정한 바, 전 3개체에 대해서 모두 정량 한계 미만이었다(정량한계: 1형은 500, 2형, 3형 및 4형은 1000; 단위는 genome copy/㎖ serum).
상기 결과에 의해 양호한 약효 및 안전성이 확인되었다.
실시예 3
1. 사이노몰거스 원숭이를 사용한 4가 뎅기 바이러스 제제의 약효ㆍ안전 평가시험 2
혈청 형별로 서로 다른 배합량(혼합 용량)을 조제한 4가 뎅기 바이러스 제제의 약효ㆍ안전성에 대한 영향을 확인할 목적으로 이하의 실험을 실시했다.
(1) 4가 뎅기 바이러스 제제 (5353 제제, 5333 제제)의 조제
VMSVP1 및 VMSVP2, VMSVP3, VMSVP4의 각각의 함유량을 5log10PFU/dose, 3log10PFU/dose, 5log10PFU/dose, 3log10PFU/dose로 한 제제(5353 제제)와, VMSVP1의 함유량을 5log10PFU/dose, VMSVP2, VMSVP3 및 VMSVP4의 함유량을 3log10PFU/dose로 한 제제(5333 제제)를 조제하고, 사이노몰거스 원숭이 3마리를 사용해서 중화 항체 응답 및 바이러스 혈증을 평가했다(각각 5353그룹, 5333그룹).
(2) Immunospot PRNT법에 의한 중화 항체가 측정
중화 항체 응답에 대해서, 5353그룹의 한 마리가 4형에 대해서만 Day 14에서는 양전하지 않고 있었던 것을 제외하고, 5353그룹 및 5333그룹 모두 Day 14 이후에서 중화 항체가 양전하고 있고, Day 60까지의 동안, 양전이 유지되고 있었다(표 6∼표 9). 또, Day 14에 4형의 양전이 인정되지 않았던 5353그룹의 한 마리에 대해서도, Day 30 이후는 양전하고 있고, 그 후 Day 60까지 양전이 유지되고 있었던 (표 14∼17).
Figure 112018061634250-pct00014
Figure 112018061634250-pct00015
Figure 112018061634250-pct00016
Figure 112018061634250-pct00017
(3) RT-qPCR법에 의한 혈중 게놈량 측정
Day 4에서 5333그룹의 한 마리에서만 혈청형 1 및 혈청형 4에 대해서 약간 정량 한계를 상회하는 바이러스 게놈이 검출되었지만(각각 1632, 2808genome/㎖), 5333그룹 및 5353그룹의 다른 개체에 대해서는 정량 한계 미만이었다. 또, Day 4에서 바이러스 게놈이 검출된 한 마리에 대해서도, 혈청형 1의 친주 및 혈청형 4의 친주를 각각 등량 단가 접종했을 경우의 개체별 피크 바이러스량의 기하평균(각각 75001(21864∼592650), 9853(3006∼32315)genome/㎖, N=3)에 비해서 낮은 값이었다.
상기 결과에 의해, 5353 제제 및 5333 제제는 5555 제제와 동등한 약효ㆍ안전성이 나타나고, 제제 중에서의 혈청 형별의 바이러스 배합량의 차이에 따라서는, 약효ㆍ안전성에 큰 영향은 없는 것이 확인되었다.
실시예 4
5555 제제, 5353 제제 및 5333 제제와는 다른 약독화 뎅기 바이러스 유래의 뎅기 바이러스를 포함하는 제제에 대해서도 평가할 목적으로 이하의 실험을 실시했다.
(1) 약독주 2B 및 약독주 4B의 제작
상술한 실시예 1의 (1) 약독주의 제작에 있어서, 혈청형 2의 임상 분리주 99345를 친주로 해서, PDK 세포에서 35 계대해서 제작한 약독화 뎅기 바이러스를 약독주 2B라고 칭한다. 또, 혈청형 4의 임상 분리주 1036을 친주로 해서, PDK 세포에서 45 계대해서 제작한 약독화 뎅기 바이러스를 약독주 4B라고 칭한다.
(2) LAVVP2B 및 LAVVP4B의 제작
약독주 2B 및 약독주 4B를 Vero 세포에서 계대하고, 각각 LAVVP2B(Live Attenuated Virus Vero Passaged 2B) 및 LAVVP4B를 얻었다.
(3) VMSVP123LAVVP4 및 VMSVP13LAVVP24의 조제
VMSVP1, VMSVP2, VMSVP3 및 LAVVP4B에 의한 4가 뎅기 바이러스 제제 (각 5log10PFU/dose; 「VMSVP123LAVVP4」로 호칭) 및 VMSVP1, LAVVP2B, VMSVP3 및 LAVVP4B에 의한 4가 뎅기 바이러스 제제 (각 5log10PFU/dose; 「VMSVP13LAVVP24」로 호칭)을 조제하고, 사이노몰거스 원숭이 각 3마리를 사용해서 중화 항체 응답 및 바이러스 혈증을 평가했다(각각 VMSVP123LAVVP4 그룹, VMSVP13LAVVP24 그룹).
(4) PRNT법에 의한 중화 항체가 측정
PRNT법에 의해, VMSVP123LAVVP4 그룹 및 VMSVP13LAVVP24 그룹의 중화 항체가를 측정했다. 그 결과, 양쪽 제제 모두 5555 제제와 동등한 중화 항체 응답을 유도했다(표 18 및 표 19).
Figure 112018061634250-pct00018
Figure 112018061634250-pct00019
(5) RT-qPCR법에 의한 혈중 게놈량 측정
바이러스 혈증에 대해서는 VMSVP123LAVVP4 그룹으로부터 비교적 높은 양의 4형의 바이러스 게놈이 Day 1에서 검출(10066genome/㎖, GMT, N=3)되고 있고, VMSVP13LAVVP24 그룹으로부터 비교적 높은 양의 4형의 바이러스 게놈과, 정량 한계를 조금이지만 상회하는 양의 1형 및 2형의 바이러스 게놈이 Day 1에서 검출(각각 12340, 547, 1337genome/㎖, GMT, N=3)되었다. 이것에 의해, VMSVP는 LAVVP에 비해서 고도로 약독화되어 있는 것이 시사되었다.
매우 흥미롭게도, VMSVP13LAVVP24 그룹의 2마리에서, Day 1에서 혈청형 1의 바이러스 게놈이 조금이지만도 검출되고 있고, VMSVP에 의한 4가 뎅기 바이러스 제제의 조합 소성의 장점을 시사하고 있는 것으로 생각되었다.
실시예 5
(1) 4가 뎅기 바이러스 제제(5555 제제)의 중화 항체가 장기 관찰 시험
양호한 약효ㆍ안전성을 나타낸 VMSVP1, VMSVP2, VMSVP3 및 VMSVP4를 사용해서 조제한 4가 뎅기 바이러스 제제(5555 제제)에 대해서, 중화 항체가의 장기 평가를 실시할 목적으로 전 6마리의 사이노몰거스 원숭이(ID No.1∼6)에 5555 제제를 단회 접종하고, 접종 월을 Month 0으로 하고, Month 1, 2, 3, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, 20, 23, 25과, 2년 이상에 걸쳐서 중화 항체가(Focus Reduction Neutralization Titer 50; 이하 「FRNT50」)을 측정했다.
그 결과, Month 1에서 모든 개체에서 중화 항체의 양전(FRNT50>10)이 인정되었다. 또, 중화 항체가의 기하 평균값은 1∼4형 어느 것에 대해서도 100 이상이었다. 중화 항체가는 그 후 2년 이상에 걸쳐서 동일 정도로 유지되고 있고, 하강의 트렌드는 인정되지 않았다(도 1∼도 4).
이것으로부터, 4가 뎅기 바이러스 제제(5555제제)는 적어도 2년 이상은 약효가 인정되고, 또 하강의 트렌드가 인정되지 않았기 때문에, 더욱 장기에 걸쳐서 중화 항체가가 유지되는 것으로 기대된다.
(2) 접종 회수 및 접종 간격의 검토
4가 뎅기 바이러스 제제(5555 제제)의 접종 회수와 접종 간격의 평가를 실시할 목적으로 1개월 간격 2회 접종, 6개월 간격 2회 접종의 스케줄로 사이노몰거스 원숭이 6마리에 본 제제를 접종했다.
그 결과, 1개월 간격 2회 접종, 6개월 간격 2회 접종의 어느 쪽의 경우에도, 중화 항체가는 단회 접종의 경우와 거의 동일 정도로 상승하고, 또, 2회 접종 이후에 중화 항체가에 큰 변화는 인정되지 않았다. 따라서 본 뎅기 바이러스 약독주를 포함하는 백신은 1회의 접종에 의해 중화 항체 응답을 충분하게 유도할 수 있음이 시사되었다. (도 5∼도 8; 도 5∼도 8은 각 5log10PFU/dose의 4가 뎅기 바이러스 제제를, 사이노몰거스 원숭이에 단회 접종(N=6), 1개월 간격 2회 접종(N=6) 및 6개월 간격 2회 접종(N=6) 했을 때의 중화 항체가의 기하평균값을 나타냈다. 수직 바는 ±SD를 나타낸다. )
(3) 투여 용량시험
추가로, 다른 용량에서의 반응성을 확인할 목적으로 각 5log10, 각 3log10, 각 2log10 및 각 1log10FFU/dose의 4가 뎅기 바이러스 제제를 조제하고, 사이노몰거스 원숭이에 단회 접종했다. 접종 주를 Week 0으로 하고, Week 2 및 Week 5의 중화 항체가를 측정했다.
그 결과, Week 2에서 전 개체의 중화 항체가 양전하고, 또 Week 5에서도 양성이 유지되고 있었다. 따라서 각 1log10FFU/dose라는 저항원량으로부터의 중화 항체 응답을 확인할 수 있었다.(도 9∼도 12; 도 9∼도 12는 각 5log10FFU/dose(N=1), 각 3log10FFU/dose(N=3), 각 2log10FFU/dose(N=3), 각 1log10FFU/dose(N=3)의 4가 뎅기 바이러스 제제를, 사이노몰거스 원숭이에 단회 접종했을 때의 Week 0, Week 2 및 Week 5의 중화 항체가의 기하평균값을 나타냈다. 수직 바는 ±SD를 나타낸다. )
실시예 6
(1) GLP 약효시험
실시예 1에서 조제한 Pre-CTM을 4가 혼합하고, 5555Log10FFU/dose, 4444Log10FFU/dose, 3333Log10FFU/dose의 4가 뎅기 백신 제제를 조제했다. 조제한 4가 뎅기 백신 제제 또는 매체를 사이노몰거스 원숭이에 피하 단회 접종하고, 중화 항체 유도 능 및 챌린지 바이러스(친주)에 대한 방어능을 평가했다.
중화 항체가는 Immunospot PRNT법에 의해 얻었다. 5555Log10FFU/dose접종 그룹(N=24), 4444Log10FFU/dose접종 그룹(N=24), 3333Log10FFU/dose접종 그룹(N=24)의 모든 개체에서 Day 14에서 중화 항체양전이 인정되었다(표 20∼28). 한편, 매체 접종 그룹에서는 중화 항체 양전은 인정되지 않았다.(표 29, 30)
챌린지 바이러스(친주)에 대한 방어능 평가를 위해서, 각 그룹 6마리씩 균등하게 추가로 그룹 분류하고, Day 60에 각각의 혈청형의 단가 친주를 피하 챌린지했다. 챌린지량은 혈청형마다 각각 5Log10PFU/dose, 5Log10PFU/dose, 6Log10PFU/dose, 5Log10PFU/dose로 했다. 챌린지 1, 2, 3, 4, 6, 8, 10, 14일 후에 혈청 중의 바이러스 게놈량을 측정한 바, 매체 접종 그룹(각 N=3)에서는 바이러스 게놈이 많이 검출되었지만(표 31), 백신 접종 그룹에서는 어느 쪽의 그룹에 있어서도 바이러스 게놈은 검출되지 않았다.
Figure 112018061634250-pct00020
Figure 112018061634250-pct00021
Figure 112018061634250-pct00022
Figure 112018061634250-pct00023
Figure 112018061634250-pct00024
Figure 112018061634250-pct00025
Figure 112018061634250-pct00026
Figure 112018061634250-pct00027
Figure 112018061634250-pct00028
Figure 112018061634250-pct00029
Figure 112018061634250-pct00030
Figure 112018061634250-pct00031
실시예 7
(1) 원숭이 신경 독력 시험
VMS를 피험물질로 해서 WHO protocol(Technical Report Series No. 978, 2013)에 따른 원숭이 신경 독력 시험을 실시했다. 1그룹당 12마리의 사이노몰거스 원숭이를 할당하고, VMS의 각 혈청형 각각 5Log10FFU/dose, 5Log10PFU/dose, 5Log10FFU/dose, 5Log10FFU/dose와, 참조 바이러스로서 Yellow Fever 17D 5Log10PFU/dose를 두개골 내에 투여했다. 투여 후, 30일 동안의 임상 스코어링 및 30일 경과 시점에서 뇌조직 및 척수에 한해서 부검을 실시했다. 그 결과, 신경 독성에 관한 문제가 있는 임상적 및 조직병리학적 소견은 인정되지 않았다.
실시예 8
(1) GLP 반복 투여 독성 시험
실시예 1에서 조제한 Pre-CTM을 4가 혼합하고, 7777Log10FFU/dose(5㎖), 5555Log10FFU/dose(0.5㎖)의 4가 뎅기 백신 제제를 조제했다. 4가 뎅기 백신 제제 또는 매체를 각각 자웅 6마리씩의 사이노몰거스 원숭이의 피하에 4주 간격으로 3회 투여하는 반복 투여 독성 시험을 실시했다. 피험물질의 독성 및 가역성을 평가할 목적으로 사이노몰거스 원숭이의 반수는 최종투여 3일후, 나머지 반수는 최종투여 28일후에 부검했다.
평가 항목은 일반 상태(Clinical signs), 행동ㆍ신경 기능(general behavior and neurobehavioral function), 국소 반응(skin reaction), 체중(body weight), 섭취량(food consumption), 체온(body temperature), 안과적 검사(ophthalmology), 심전도(electrocardiography), 호흡수(respiratory rate), 요검사(urinalysis), 혈액학적검사(hematology), 혈액생화학적 검사(blood chemistry), 부검(necropsy), 기관중량(organ weights), 병리조직학적 검사(histopathology), 정소세포 수 평가(quantitative examination of testes)로 했다.
반복 독성 시험의 투여기간 및 회복기간 중에 사망한 개체는 어느 쪽의 투여그룹에 있어서도 인정되지 않았다. 또, 투여기간 및 회복기간 중에, 일반 상태, 행동ㆍ신경 기능, 국소 반응, 체중, 섭취량, 체온, 안과적 검사, 심전도, 호흡수, 요검사, 부검, 기관중량, 정소 세포 수 평가에 있어서, 피험물질에 관련되는 변화는 자웅 모두 어느 쪽의 투여그룹에 있어서도 인정되지 않았다.
혈액학적으로는 피험물질 투여에 의해 백혈구, 호중구, 림프구, 단구, 호염구, 대형 비염색 세포의 개수 증가가 일부 개체에서 볼 수 있었지만, 접종 부위의 염증 또는 면역응답에 기인하는 이차적인 변화로, 독성학적으로 유의한 것이 아니라고 생각되었다. 병리조직학적으로는 접종 부위에만 혈관 주위에 염증성 세포 침윤이 극소 정도에서 중정도 볼 수 있었지만, 그것들은 3회째의 투여로부터 4주 사이에 회복 또는 회복 경향을 나타냈다.
이상에서, 7777Log10FFU/dose가 당해 시험에서의 no-observed-adverse-effectlevel이라고 결론지을 수 있었다.
(산업상의 이용가능성)
본 발명은 뎅기 바이러스를 사용한 백신으로 이용 가능하다.
SEQUENCE LISTING <110> The Chemo-Sero-Therapeutic Research Institute <120> Live virus having a bank of dengue virus attenuated strains, and a dengue vaccine containing same as antigens <130> 673093 <140> JP2015-232013 <141> 2015-11-27 <160> 32 <170> PatentIn version 3.5 <210> 1 <211> 3392 <212> PRT <213> Dengue virus type 1 <220> <221> VARIANT <222> (199)..(199) <223> Xaa stands for Lys or Glu <220> <221> VARIANT <222> (999)..(999) <223> Xaa stands for Val or Ile <220> <221> VARIANT <222> (2353)..(2353) <223> Xaa stands for Thr or Ile <220> <221> VARIANT <222> (2360)..(2360) <223> Xaa stands for Val or Ala <400> 1 Met Asn Asn Gln Arg Lys Lys Thr Ala Arg Pro Ser Phe Asn Met Leu 1 5 10 15 Lys Arg Ala Arg Asn Arg Val Ser Thr Val Ser Gln Leu Ala Lys Arg 20 25 30 Phe Ser Lys Gly Leu Leu Ser Gly Gln Gly Pro Met Lys Leu Val Met 35 40 45 Ala Phe Ile Ala Phe Leu Arg Phe Leu Ala Ile Pro Pro Thr Ala Gly 50 55 60 Ile Leu Ala Arg Trp Gly Ser Phe Lys Lys Asn Gly Ala Ile Lys Val 65 70 75 80 Leu Arg Gly Phe Lys Lys Glu Ile Ser Asn Met Leu Asn Ile Met Asn 85 90 95 Arg Arg Lys Arg Ser Val Thr Met Leu Leu Met Leu Met Pro Thr Ala 100 105 110 Leu Ala Phe His Leu Thr Thr Arg Gly Gly Glu Pro His Met Ile Val 115 120 125 Ser Lys Gln Glu Arg Gly Lys Ser Leu Leu Phe Lys Thr Ser Ala Gly 130 135 140 Val Asn Met Cys Thr Leu Ile Ala Met Asp Leu Gly Glu Leu Cys Glu 145 150 155 160 Asp Thr Met Thr Tyr Lys Cys Pro Arg Ile Thr Glu Ala Glu Pro Asp 165 170 175 Asp Val Asp Cys Trp Cys Asn Ala Thr Asp Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Ser Gln Thr Gly Xaa His Arg Arg Asp Lys Arg Ser Val Ala 195 200 205 Leu Ala Pro His Val Gly Leu Gly Leu Glu Thr Arg Thr Glu Thr Trp 210 215 220 Met Ser Ser Glu Gly Ala Trp Lys Gln Ile Gln Arg Val Glu Thr Trp 225 230 235 240 Ala Leu Arg His Pro Gly Phe Thr Val Ile Ala Leu Phe Leu Ala His 245 250 255 Ala Ile Gly Thr Ser Ile Thr Gln Lys Gly Ile Ile Phe Ile Leu Leu 260 265 270 Met Leu Val Thr Pro Ser Met Ala Met Arg Cys Val Gly Ile Gly Ser 275 280 285 Arg Asp Phe Val Glu Gly Leu Ser Gly Ala Thr Trp Val Asp Val Val 290 295 300 Leu Glu His Gly Ser Cys Val Thr Thr Met Ala Lys Asp Lys Pro Thr 305 310 315 320 Leu Asp Ile Glu Leu Leu Lys Thr Glu Val Thr Asn Pro Ala Val Leu 325 330 335 Arg Lys Leu Cys Ile Glu Ala Lys Ile Ser Asn Thr Thr Thr Asp Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Ala Thr Leu Val Glu Glu Gln Asp Ala 355 360 365 Asn Phe Val Cys Arg Arg Thr Phe Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Ser Leu Leu Thr Cys Ala Lys Phe Lys 385 390 395 400 Cys Val Thr Lys Leu Glu Gly Lys Ile Val Gln Tyr Glu Asn Leu Lys 405 410 415 Tyr Ser Val Ile Val Thr Val His Thr Gly Asp Gln His Gln Val Gly 420 425 430 Asn Glu Ser Thr Glu His Gly Thr Thr Ala Thr Ile Thr Pro Gln Ala 435 440 445 Pro Thr Thr Glu Ile Gln Leu Thr Asp Tyr Gly Ala Leu Thr Leu Asp 450 455 460 Cys Ser Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Val Leu Leu Thr 465 470 475 480 Met Lys Glu Lys Ser Trp Leu Val His Lys Gln Trp Phe Leu Asp Leu 485 490 495 Pro Leu Pro Trp Thr Ser Gly Ala Ser Thr Ser Gln Glu Thr Trp Asn 500 505 510 Arg Gln Asp Leu Leu Val Thr Phe Lys Thr Ala His Ala Lys Lys Gln 515 520 525 Glu Val Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu 530 535 540 Thr Gly Ala Thr Glu Ile Gln Thr Ser Gly Thr Thr Thr Ile Phe Ala 545 550 555 560 Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Thr Leu Lys Gly 565 570 575 Met Ser Tyr Val Met Cys Thr Gly Ser Phe Lys Leu Glu Lys Glu Val 580 585 590 Ala Glu Thr Gln His Gly Thr Val Leu Val Gln Ile Lys Tyr Glu Gly 595 600 605 Thr Asp Ala Pro Cys Lys Ile Pro Phe Ser Thr Gln Asp Glu Lys Gly 610 615 620 Val Thr Gln Asn Gly Arg Leu Ile Thr Ala Asn Pro Ile Val Thr Asp 625 630 635 640 Lys Glu Lys Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser 645 650 655 Tyr Ile Val Ile Gly Ala Gly Glu Lys Ala Leu Lys Leu Ser Trp Phe 660 665 670 Lys Lys Gly Ser Ser Ile Gly Lys Met Phe Glu Ala Thr Ala Arg Gly 675 680 685 Ala Arg Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 690 695 700 Ile Gly Gly Val Phe Thr Ser Val Gly Lys Leu Val His Gln Ile Phe 705 710 715 720 Gly Thr Ala Tyr Gly Val Leu Phe Ser Gly Val Ser Trp Thr Met Lys 725 730 735 Ile Gly Ile Gly Val Leu Leu Thr Trp Leu Gly Leu Asn Ser Arg Asn 740 745 750 Thr Ser Leu Ser Met Thr Cys Ile Ala Val Gly Leu Val Thr Leu Tyr 755 760 765 Leu Gly Val Met Val Gln Ala Asp Ser Gly Cys Val Ile Asn Trp Lys 770 775 780 Gly Arg Glu Leu Lys Cys Gly Ser Gly Ile Phe Val Thr Asn Glu Val 785 790 795 800 His Thr Trp Thr Glu Gln Tyr Lys Phe Gln Ala Asp Ser Pro Lys Arg 805 810 815 Leu Ser Ala Ala Ile Gly Lys Ala Trp Glu Glu Gly Val Cys Gly Ile 820 825 830 Arg Ser Ala Thr Arg Leu Glu Asn Ile Met Trp Lys Gln Ile Ser Asn 835 840 845 Glu Leu Asn His Ile Leu Leu Glu Asn Asp Met Lys Phe Thr Val Val 850 855 860 Val Gly Asp Val Ala Gly Ile Leu Ala Gln Gly Lys Lys Met Ile Arg 865 870 875 880 Pro Gln Pro Met Glu His Lys Tyr Ser Trp Lys Ser Trp Gly Lys Ala 885 890 895 Lys Ile Ile Gly Ala Asp Val Gln Asn Ser Thr Phe Ile Ile Asp Gly 900 905 910 Pro Asn Thr Pro Glu Cys Pro Asp Asp Gln Arg Ala Trp Asn Ile Trp 915 920 925 Glu Val Glu Asp Tyr Gly Phe Gly Ile Phe Thr Thr Asn Ile Trp Leu 930 935 940 Lys Leu Arg Asp Ser Tyr Thr Gln Leu Cys Asp His Arg Leu Met Ser 945 950 955 960 Ala Ala Ile Lys Asp Ser Lys Ala Val His Ala Asp Met Gly Tyr Trp 965 970 975 Ile Glu Ser Glu Lys Asn Glu Thr Trp Lys Leu Ala Arg Ala Ser Phe 980 985 990 Ile Glu Val Lys Thr Cys Xaa Trp Pro Lys Ser His Thr Leu Trp Ser 995 1000 1005 Asn Gly Val Leu Glu Ser Glu Met Ile Ile Pro Lys Ile Tyr Gly 1010 1015 1020 Gly Pro Ile Ser Gln His Asn Tyr Arg Pro Gly Tyr Phe Thr Gln 1025 1030 1035 Thr Val Gly Pro Trp His Leu Gly Lys Leu Glu Leu Asp Phe Asp 1040 1045 1050 Leu Cys Glu Gly Thr Thr Val Val Val Asp Glu His Cys Gly Asn 1055 1060 1065 Arg Gly Pro Ser Leu Arg Thr Thr Thr Val Thr Gly Lys Ile Ile 1070 1075 1080 His Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Phe 1085 1090 1095 Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Val 1100 1105 1110 Lys Glu Lys Glu Glu Asn Leu Val Lys Ser Met Val Ser Ala Gly 1115 1120 1125 Ser Gly Glu Val Asp Ser Phe Ser Leu Gly Leu Leu Cys Ile Ser 1130 1135 1140 Ile Met Ile Glu Glu Val Met Arg Ser Arg Trp Ser Arg Lys Met 1145 1150 1155 Leu Met Thr Gly Thr Leu Ala Val Phe Leu Leu Leu Ile Met Gly 1160 1165 1170 Gln Leu Thr Trp Asn Asp Leu Ile Arg Leu Cys Ile Met Val Gly 1175 1180 1185 Ala Asn Ala Ser Asp Arg Met Gly Met Gly Thr Thr Tyr Leu Ala 1190 1195 1200 Leu Met Ala Thr Phe Lys Met Arg Pro Met Phe Ala Val Gly Leu 1205 1210 1215 Leu Phe Arg Arg Leu Thr Ser Arg Glu Val Leu Leu Leu Thr Ile 1220 1225 1230 Gly Leu Ser Leu Val Ala Ser Val Glu Leu Pro Asn Ser Leu Glu 1235 1240 1245 Glu Leu Gly Asp Gly Leu Ala Met Gly Ile Met Ile Leu Lys Leu 1250 1255 1260 Leu Thr Asp Phe Gln Ser His Gln Leu Trp Ala Thr Leu Leu Ser 1265 1270 1275 Leu Thr Phe Ile Lys Thr Thr Phe Ser Leu His Tyr Ala Trp Lys 1280 1285 1290 Thr Met Ala Met Val Leu Ser Ile Val Ser Leu Phe Pro Leu Cys 1295 1300 1305 Leu Ser Thr Thr Ser Gln Lys Thr Thr Trp Leu Pro Val Leu Leu 1310 1315 1320 Gly Ser Leu Gly Cys Lys Pro Leu Thr Met Phe Leu Ile Ala Glu 1325 1330 1335 Asn Lys Ile Trp Gly Arg Lys Ser Trp Pro Leu Asn Glu Gly Ile 1340 1345 1350 Met Ala Val Gly Ile Val Ser Ile Leu Leu Ser Ser Leu Leu Lys 1355 1360 1365 Asn Asp Val Pro Leu Ala Gly Pro Leu Ile Ala Gly Gly Met Leu 1370 1375 1380 Ile Ala Cys Tyr Val Ile Ser Gly Ser Ser Ala Asp Leu Ser Leu 1385 1390 1395 Glu Lys Ala Ala Glu Val Ser Trp Glu Glu Glu Ala Glu His Ser 1400 1405 1410 Gly Ala Ser His Asn Ile Leu Val Glu Val Gln Asp Asp Gly Thr 1415 1420 1425 Met Lys Ile Lys Asp Glu Glu Arg Asp Asp Thr Leu Thr Ile Leu 1430 1435 1440 Leu Lys Ala Thr Leu Leu Ala Val Ser Gly Val Tyr Pro Leu Ser 1445 1450 1455 Ile Pro Ala Thr Leu Phe Val Trp Tyr Phe Trp Gln Lys Lys Lys 1460 1465 1470 Gln Arg Ser Gly Val Leu Trp Asp Thr Pro Ser Pro Pro Glu Val 1475 1480 1485 Glu Arg Ala Val Leu Asp Asp Gly Ile Tyr Arg Ile Met Gln Arg 1490 1495 1500 Gly Leu Leu Gly Arg Ser Gln Val Gly Val Gly Val Phe Gln Glu 1505 1510 1515 Asn Val Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu 1520 1525 1530 Met Tyr Gln Gly Lys Arg Leu Glu Pro Ser Trp Ala Ser Val Lys 1535 1540 1545 Lys Asp Leu Ile Ser Tyr Gly Gly Gly Trp Arg Leu Gln Gly Ser 1550 1555 1560 Trp Asn Thr Gly Glu Glu Val Gln Val Ile Ala Val Glu Pro Gly 1565 1570 1575 Lys Asn Pro Lys Asn Val Gln Thr Ala Pro Gly Thr Phe Lys Thr 1580 1585 1590 Pro Glu Gly Glu Val Gly Ala Ile Ala Leu Asp Phe Lys Pro Gly 1595 1600 1605 Thr Ser Gly Ser Pro Ile Val Asn Arg Glu Gly Lys Ile Val Gly 1610 1615 1620 Leu Tyr Gly Asn Gly Val Val Thr Thr Ser Gly Thr Tyr Val Ser 1625 1630 1635 Ala Ile Ala Gln Ala Lys Ala Ser Gln Glu Gly Pro Leu Pro Glu 1640 1645 1650 Ile Glu Asp Glu Val Phe Arg Lys Arg Asn Leu Thr Ile Met Asp 1655 1660 1665 Leu His Pro Gly Ser Gly Lys Thr Arg Arg Tyr Leu Pro Ala Ile 1670 1675 1680 Val Arg Glu Ala Ile Lys Arg Lys Leu Arg Thr Leu Ile Leu Ala 1685 1690 1695 Pro Thr Arg Val Val Ala Ser Glu Met Ala Glu Ala Leu Lys Gly 1700 1705 1710 Met Pro Ile Arg Tyr Gln Thr Thr Ala Val Lys Ser Glu His Thr 1715 1720 1725 Gly Lys Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met 1730 1735 1740 Arg Leu Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Met Ile Ile 1745 1750 1755 Met Asp Glu Ala His Phe Thr Asp Pro Ser Ser Ile Ala Ala Arg 1760 1765 1770 Gly Tyr Ile Ser Thr Arg Val Gly Met Gly Glu Ala Ala Ala Ile 1775 1780 1785 Phe Met Thr Ala Thr Pro Pro Gly Ser Val Glu Ala Phe Pro Gln 1790 1795 1800 Ser Asn Ala Val Ile Gln Asp Glu Glu Arg Asp Ile Pro Glu Arg 1805 1810 1815 Ser Trp Asn Ser Gly Tyr Glu Trp Ile Thr Asp Phe Pro Gly Lys 1820 1825 1830 Thr Val Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala 1835 1840 1845 Asn Cys Leu Arg Lys Asn Gly Lys Arg Val Ile Gln Leu Ser Arg 1850 1855 1860 Lys Thr Phe Asp Thr Glu Tyr Gln Lys Thr Lys Asn Asn Asp Trp 1865 1870 1875 Asp Tyr Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe 1880 1885 1890 Arg Ala Asp Arg Val Ile Asp Pro Arg Arg Cys Leu Lys Pro Val 1895 1900 1905 Ile Leu Lys Asp Gly Pro Glu Arg Val Ile Leu Ala Gly Pro Met 1910 1915 1920 Pro Val Thr Val Ala Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly 1925 1930 1935 Arg Asn His Asn Lys Glu Gly Asp Gln Tyr Ile Tyr Met Gly Gln 1940 1945 1950 Pro Leu Asn Asn Asp Glu Asp His Ala His Trp Thr Glu Ala Lys 1955 1960 1965 Met Leu Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ala 1970 1975 1980 Leu Phe Glu Pro Glu Arg Glu Lys Ser Ala Ala Ile Asp Gly Glu 1985 1990 1995 Tyr Arg Leu Arg Gly Glu Ala Arg Lys Thr Phe Val Glu Leu Met 2000 2005 2010 Arg Arg Gly Asp Leu Pro Val Trp Leu Ser Tyr Lys Val Ala Ser 2015 2020 2025 Glu Gly Phe Gln Tyr Ser Asp Arg Arg Trp Cys Phe Asp Gly Glu 2030 2035 2040 Arg Asn Asn Gln Val Leu Glu Glu Asn Met Asp Val Glu Ile Trp 2045 2050 2055 Thr Lys Glu Gly Glu Arg Lys Lys Leu Arg Pro Arg Trp Leu Asp 2060 2065 2070 Ala Arg Thr Tyr Ser Asp Pro Leu Ala Leu Arg Glu Phe Lys Glu 2075 2080 2085 Phe Ala Ala Gly Arg Arg Ser Val Ser Gly Asp Leu Ile Leu Glu 2090 2095 2100 Ile Gly Lys Leu Pro Gln His Leu Thr Gln Arg Ala Gln Asn Ala 2105 2110 2115 Leu Asp Asn Leu Val Met Leu His Asn Ser Glu Gln Gly Gly Arg 2120 2125 2130 Ala Tyr Arg His Ala Met Glu Glu Leu Pro Asp Thr Ile Glu Thr 2135 2140 2145 Leu Met Leu Leu Ala Leu Ile Ala Val Leu Thr Gly Gly Val Thr 2150 2155 2160 Leu Phe Phe Leu Ser Gly Lys Gly Leu Gly Lys Thr Ser Ile Gly 2165 2170 2175 Leu Leu Cys Val Met Ala Ser Ser Val Leu Leu Trp Met Ala Ser 2180 2185 2190 Val Glu Pro His Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe 2195 2200 2205 Leu Met Val Leu Leu Ile Pro Glu Pro Asp Arg Gln Arg Thr Pro 2210 2215 2220 Gln Asp Asn Gln Leu Ala Tyr Val Val Ile Gly Leu Leu Phe Met 2225 2230 2235 Ile Leu Thr Val Ala Ala Asn Glu Met Gly Leu Leu Glu Thr Thr 2240 2245 2250 Lys Lys Asp Leu Gly Ile Gly His Val Ala Val Glu Asn His His 2255 2260 2265 His Ala Ala Met Leu Asp Val Asp Leu His Pro Ala Ser Ala Trp 2270 2275 2280 Thr Leu Tyr Ala Val Ala Thr Thr Ile Ile Thr Pro Met Met Arg 2285 2290 2295 His Thr Ile Glu Asn Thr Thr Ala Asn Ile Ser Leu Thr Ala Ile 2300 2305 2310 Ala Asn Gln Ala Ala Ile Leu Met Gly Leu Asp Lys Gly Trp Pro 2315 2320 2325 Ile Ser Lys Met Asp Ile Gly Val Pro Leu Leu Ala Leu Gly Cys 2330 2335 2340 Tyr Ser Gln Val Asn Pro Leu Thr Leu Xaa Ala Ala Val Leu Met 2345 2350 2355 Leu Xaa Ala His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys 2360 2365 2370 Ala Thr Arg Glu Ala Gln Lys Arg Thr Ala Ala Gly Ile Met Lys 2375 2380 2385 Asn Pro Thr Ile Asp Gly Ile Val Ala Ile Asp Leu Asp Pro Val 2390 2395 2400 Val Tyr Asp Ala Lys Phe Glu Lys Gln Leu Gly Gln Ile Met Leu 2405 2410 2415 Leu Ile Leu Cys Thr Ser Gln Ile Leu Leu Met Arg Thr Thr Trp 2420 2425 2430 Ala Leu Cys Glu Ser Ile Thr Leu Ala Thr Gly Pro Leu Thr Thr 2435 2440 2445 Leu Trp Glu Gly Ser Pro Gly Lys Phe Trp Asn Thr Thr Ile Ala 2450 2455 2460 Val Ser Met Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala 2465 2470 2475 Gly Leu Ala Phe Ser Leu Met Lys Ser Leu Gly Gly Gly Arg Arg 2480 2485 2490 Gly Thr Gly Ala Gln Gly Glu Thr Leu Gly Glu Lys Trp Lys Arg 2495 2500 2505 Gln Leu Asn Gln Leu Ser Lys Ser Glu Phe Asn Thr Tyr Lys Arg 2510 2515 2520 Ser Gly Ile Met Glu Val Asp Arg Ser Glu Ala Lys Glu Gly Leu 2525 2530 2535 Lys Arg Gly Glu Thr Thr Lys His Ala Val Ser Arg Gly Thr Ala 2540 2545 2550 Lys Leu Arg Trp Phe Val Glu Arg Asn Leu Val Lys Pro Glu Gly 2555 2560 2565 Lys Val Ile Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr 2570 2575 2580 Cys Ala Gly Leu Lys Lys Val Thr Glu Val Lys Gly Tyr Thr Lys 2585 2590 2595 Gly Gly Pro Gly His Glu Glu Pro Ile Pro Met Ala Thr Tyr Gly 2600 2605 2610 Trp Asn Leu Val Lys Leu His Ser Gly Lys Asp Val Phe Phe Ile 2615 2620 2625 Pro Pro Glu Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser 2630 2635 2640 Ser Pro Asn Pro Thr Ile Glu Glu Gly Arg Thr Leu Arg Val Leu 2645 2650 2655 Lys Met Val Glu Pro Trp Leu Arg Gly Asn Gln Phe Cys Ile Lys 2660 2665 2670 Ile Leu Asn Pro Tyr Met Pro Ser Val Val Glu Thr Leu Glu Gln 2675 2680 2685 Met Gln Arg Lys His Gly Gly Met Leu Val Arg Asn Pro Leu Ser 2690 2695 2700 Arg Asn Ser Thr His Glu Met Tyr Trp Val Ser Cys Gly Thr Gly 2705 2710 2715 Asn Ile Val Ser Ala Val Asn Met Thr Ser Arg Met Leu Leu Asn 2720 2725 2730 Arg Phe Thr Met Ala His Arg Lys Pro Thr Tyr Glu Arg Asp Val 2735 2740 2745 Asp Leu Gly Ala Gly Thr Arg His Val Ala Val Glu Pro Glu Val 2750 2755 2760 Ala Asn Leu Asp Ile Ile Gly Gln Arg Ile Glu Asn Ile Lys His 2765 2770 2775 Glu His Lys Ser Thr Trp His Tyr Asp Glu Asp Asn Pro Tyr Lys 2780 2785 2790 Thr Trp Ala Tyr His Gly Ser Tyr Glu Val Lys Pro Ser Gly Ser 2795 2800 2805 Ala Ser Ser Met Val Asn Gly Val Val Lys Leu Leu Thr Lys Pro 2810 2815 2820 Trp Asp Val Ile Pro Met Val Thr Gln Ile Ala Met Thr Asp Thr 2825 2830 2835 Thr Pro Phe Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr 2840 2845 2850 Arg Thr Pro Lys Ala Lys Arg Gly Thr Ala Gln Ile Met Glu Val 2855 2860 2865 Thr Ala Arg Trp Leu Trp Gly Phe Leu Ser Arg Asn Lys Lys Pro 2870 2875 2880 Arg Ile Cys Thr Arg Glu Glu Phe Thr Arg Lys Val Arg Ser Asn 2885 2890 2895 Ala Ala Ile Gly Ala Val Phe Val Asp Glu Asn Gln Trp Asn Ser 2900 2905 2910 Ala Lys Glu Ala Val Glu Asp Asp Arg Phe Trp Asp Leu Val His 2915 2920 2925 Arg Glu Arg Glu Leu His Lys Gln Gly Lys Cys Ala Thr Cys Val 2930 2935 2940 Tyr Asn Met Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly 2945 2950 2955 Lys Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala 2960 2965 2970 Arg Phe Leu Glu Phe Glu Ala Leu Gly Phe Met Asn Glu Asp His 2975 2980 2985 Trp Phe Ser Arg Glu Asn Ser Leu Ser Gly Val Glu Gly Glu Gly 2990 2995 3000 Leu His Lys Leu Gly Tyr Ile Leu Arg Asp Ile Ser Lys Ile Pro 3005 3010 3015 Gly Gly Asn Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg 3020 3025 3030 Ile Thr Glu Asp Asp Leu Gln Asn Glu Ala Lys Ile Thr Asp Ile 3035 3040 3045 Met Glu Pro Glu His Ala Leu Leu Ala Lys Ser Ile Phe Lys Leu 3050 3055 3060 Thr Tyr Gln Asn Lys Val Val Arg Val Gln Arg Pro Ala Lys Asn 3065 3070 3075 Gly Thr Val Met Asp Val Ile Ser Arg Arg Asp Gln Arg Gly Ser 3080 3085 3090 Gly Gln Val Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu 3095 3100 3105 Ala Gln Leu Ile Arg Gln Met Glu Ser Glu Gly Ile Phe Ser Pro 3110 3115 3120 Asn Glu Leu Glu Thr Pro Asn Leu Ala Glu Arg Val Leu Asp Trp 3125 3130 3135 Leu Glu Lys Tyr Gly Val Glu Arg Leu Lys Arg Met Ala Ile Ser 3140 3145 3150 Gly Asp Asp Cys Val Val Lys Pro Ile Asp Asp Arg Phe Ala Thr 3155 3160 3165 Ala Leu Thr Ala Leu Asn Asp Met Gly Lys Val Arg Lys Asp Ile 3170 3175 3180 Pro Gln Trp Glu Pro Ser Lys Gly Trp Asn Asp Trp Gln Gln Val 3185 3190 3195 Pro Phe Cys Ser His His Phe His Gln Leu Ile Met Lys Asp Gly 3200 3205 3210 Arg Glu Ile Val Val Pro Cys Arg Asn Gln Asp Glu Leu Val Gly 3215 3220 3225 Arg Ala Arg Val Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr 3230 3235 3240 Ala Cys Leu Gly Lys Ser Tyr Ala Gln Met Trp Gln Leu Met Tyr 3245 3250 3255 Phe His Arg Arg Asp Leu Arg Leu Ala Ala Asn Ala Ile Cys Ser 3260 3265 3270 Ala Val Pro Val Asp Trp Val Pro Thr Ser Arg Thr Thr Trp Ser 3275 3280 3285 Ile His Ala His His Gln Trp Met Thr Thr Glu Asp Met Leu Ser 3290 3295 3300 Val Trp Asn Arg Val Trp Ile Glu Glu Asn Pro Trp Met Glu Asp 3305 3310 3315 Lys Thr Arg Ile Ser Ser Trp Glu Asp Val Pro Tyr Leu Gly Lys 3320 3325 3330 Arg Glu Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ala Arg 3335 3340 3345 Ala Thr Trp Ala Thr Asn Ile Gln Val Ala Ile Asn Gln Val Arg 3350 3355 3360 Arg Leu Ile Gly Asn Glu Asn Tyr Ser Asp Tyr Met Thr Ser Met 3365 3370 3375 Lys Arg Phe Lys Asn Glu Ser Glu Pro Glu Gly Ala Leu Trp 3380 3385 3390 <210> 2 <211> 3391 <212> PRT <213> Dengue virus type 2 <220> <221> VARIANT <222> (398)..(398) <223> Xaa stands for Lys or Met <220> <221> VARIANT <222> (484)..(484) <223> Xaa stands for Lys or Arg <400> 2 Met Asn Asn Gln Arg Lys Lys Ala Lys Asn Thr Pro Phe Asn Met Leu 1 5 10 15 Lys Arg Glu Arg Asn Arg Val Ser Thr Val Gln Gln Leu Thr Lys Arg 20 25 30 Phe Ser Leu Gly Met Leu Gln Gly Arg Gly Pro Leu Lys Leu Phe Met 35 40 45 Ala Leu Val Ala Phe Leu Arg Phe Leu Thr Ile Pro Pro Thr Ala Gly 50 55 60 Ile Leu Lys Arg Trp Gly Thr Ile Lys Lys Ser Lys Ala Ile Asn Val 65 70 75 80 Leu Arg Gly Phe Arg Lys Glu Ile Gly Arg Met Leu Asn Ile Leu Asn 85 90 95 Arg Arg Arg Arg Ser Ala Gly Met Ile Ile Met Leu Ile Pro Thr Val 100 105 110 Met Ala Phe His Leu Thr Thr Arg Asn Gly Glu Pro His Met Ile Val 115 120 125 Gly Ile Gln Glu Lys Gly Lys Ser Leu Leu Phe Lys Thr Glu Asp Gly 130 135 140 Val Asn Met Cys Thr Leu Met Ala Met Asp Leu Gly Glu Leu Cys Glu 145 150 155 160 Asp Thr Ile Thr Tyr Lys Cys Pro Leu Leu Arg Gln Asn Glu Pro Glu 165 170 175 Asp Ile Asp Cys Trp Cys Asn Ser Thr Ser Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Thr Thr Thr Gly Glu His Arg Arg Glu Lys Arg Ser Val Ala 195 200 205 Leu Val Pro His Val Gly Met Gly Leu Glu Thr Arg Thr Glu Thr Trp 210 215 220 Met Ser Ser Glu Gly Ala Trp Lys His Ala Gln Arg Ile Glu Thr Trp 225 230 235 240 Ile Leu Arg His Pro Gly Phe Thr Ile Met Ala Ala Ile Leu Ala Tyr 245 250 255 Thr Ile Gly Thr Thr His Phe Gln Arg Val Leu Ile Phe Ile Leu Leu 260 265 270 Thr Ala Val Ala Pro Ser Met Thr Met Arg Cys Ile Gly Ile Ser Asn 275 280 285 Arg Asp Phe Val Glu Gly Val Ser Gly Gly Ser Trp Val Asp Ile Val 290 295 300 Leu Glu His Gly Ser Cys Val Thr Thr Met Ala Lys Asn Lys Pro Thr 305 310 315 320 Leu Asp Phe Glu Leu Ile Lys Thr Glu Ala Lys Gln Pro Ala Thr Leu 325 330 335 Arg Lys Tyr Cys Ile Glu Ala Lys Leu Thr Asn Thr Thr Thr Glu Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Pro Ser Leu Lys Glu Glu Gln Asp Lys 355 360 365 Arg Phe Val Cys Lys His Ser Met Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Gly Ile Val Thr Cys Ala Xaa Phe Thr 385 390 395 400 Cys Lys Lys Asn Met Glu Gly Lys Ile Val Gln Pro Glu Asn Leu Glu 405 410 415 Tyr Thr Ile Val Val Thr Pro His Ser Gly Glu Glu His Ala Val Gly 420 425 430 Asn Asp Thr Gly Lys His Gly Lys Glu Ile Lys Val Thr Pro Gln Ser 435 440 445 Ser Ile Thr Glu Ala Glu Leu Thr Gly Tyr Gly Thr Val Thr Met Glu 450 455 460 Cys Ser Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Val Leu Leu Gln 465 470 475 480 Met Glu Asn Xaa Ala Trp Leu Val His Arg Gln Trp Phe Leu Asp Leu 485 490 495 Pro Leu Pro Trp Leu Pro Gly Ala Asp Lys Gln Glu Ser Asn Trp Ile 500 505 510 Gln Lys Glu Thr Leu Val Thr Phe Lys Asn Pro His Ala Lys Lys Gln 515 520 525 Asp Val Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu 530 535 540 Thr Gly Ala Thr Glu Ile Gln Met Ser Ser Gly Asn Leu Leu Phe Thr 545 550 555 560 Gly His Leu Lys Cys Arg Leu Arg Met Asp Lys Leu Gln Leu Lys Gly 565 570 575 Met Ser Tyr Ser Met Cys Thr Gly Lys Phe Lys Val Val Lys Glu Ile 580 585 590 Ala Glu Thr Gln His Gly Thr Ile Val Ile Arg Val Gln Tyr Glu Gly 595 600 605 Asp Gly Ser Pro Cys Lys Ile Pro Phe Glu Ile Met Asp Leu Glu Lys 610 615 620 Arg Tyr Val Leu Gly Arg Leu Ile Thr Val Asn Pro Ile Val Thr Glu 625 630 635 640 Lys Asp Ser Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Asp Ser 645 650 655 Tyr Ile Ile Ile Gly Val Glu Pro Gly Gln Leu Lys Leu Asn Trp Phe 660 665 670 Lys Lys Gly Ser Ser Ile Gly Gln Met Phe Glu Thr Thr Met Arg Gly 675 680 685 Ala Lys Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 690 695 700 Leu Gly Gly Val Phe Thr Ser Ile Gly Lys Ala Leu His Gln Val Phe 705 710 715 720 Gly Ala Ile Tyr Gly Ala Ala Phe Ser Gly Val Ser Trp Thr Met Lys 725 730 735 Ile Leu Ile Gly Val Ile Ile Thr Trp Ile Gly Met Asn Ser Arg Ser 740 745 750 Thr Ser Leu Ser Val Ser Leu Val Leu Val Gly Ile Val Thr Leu Tyr 755 760 765 Leu Gly Val Met Val Gln Ala Asp Ser Gly Cys Val Val Ser Trp Lys 770 775 780 Asn Lys Glu Leu Lys Cys Gly Ser Gly Ile Phe Ile Thr Asp Asn Val 785 790 795 800 His Thr Trp Thr Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ser Lys 805 810 815 Leu Ala Ser Ala Ile Gln Lys Ala Gln Glu Glu Gly Ile Cys Gly Ile 820 825 830 Arg Ser Val Thr Arg Leu Glu Asn Leu Met Trp Lys Gln Ile Thr Pro 835 840 845 Glu Leu Asn His Ile Leu Ala Glu Asn Glu Val Lys Leu Thr Ile Met 850 855 860 Thr Gly Asp Ile Lys Gly Ile Met Gln Ala Gly Lys Arg Ser Leu Arg 865 870 875 880 Pro Gln Pro Thr Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala 885 890 895 Lys Met Leu Ser Thr Glu Ser Tyr Asn Gln Thr Phe Leu Ile Asp Gly 900 905 910 Pro Glu Thr Ala Glu Cys Pro Asn Thr Asn Arg Ala Trp Asn Ser Leu 915 920 925 Glu Val Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu 930 935 940 Lys Leu Lys Glu Lys Gln Asp Ala Phe Cys Asp Ser Lys Leu Met Ser 945 950 955 960 Ala Ala Ile Lys Asp Asn Arg Ala Val His Ala Asp Met Gly Tyr Trp 965 970 975 Ile Glu Ser Ala Leu Asn Asp Thr Trp Lys Ile Glu Lys Ala Ser Phe 980 985 990 Ile Glu Val Lys Asn Cys His Trp Pro Lys Ser His Thr Leu Trp Ser 995 1000 1005 Asn Gly Val Leu Glu Ser Glu Met Ile Ile Pro Lys Asn Leu Ala 1010 1015 1020 Gly Pro Val Ser Gln His Asn Tyr Arg Pro Gly Tyr His Thr Gln 1025 1030 1035 Val Ala Gly Pro Trp His Leu Gly Lys Leu Glu Met Asp Phe Asp 1040 1045 1050 Phe Cys Asp Gly Thr Thr Val Ile Val Thr Glu Asp Cys Gly Asn 1055 1060 1065 Arg Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys Leu Ile 1070 1075 1080 Thr Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Tyr 1085 1090 1095 Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu 1100 1105 1110 Lys Glu Lys Glu Glu Asn Leu Val Asn Ser Leu Val Thr Ala Gly 1115 1120 1125 His Gly Gln Val Asp Asn Phe Ser Leu Gly Val Leu Gly Met Ala 1130 1135 1140 Leu Phe Leu Glu Glu Met Leu Arg Thr Arg Val Gly Thr Lys His 1145 1150 1155 Ala Ile Leu Leu Val Ala Val Ser Phe Val Thr Leu Ile Thr Gly 1160 1165 1170 Asn Met Ser Phe Lys Asp Leu Gly Arg Val Val Val Met Val Gly 1175 1180 1185 Ala Thr Met Ala Asp Asp Ile Gly Met Gly Val Thr Tyr Leu Ala 1190 1195 1200 Leu Leu Ala Ala Phe Lys Val Arg Pro Thr Phe Ala Ala Gly Leu 1205 1210 1215 Leu Leu Arg Lys Leu Thr Ser Lys Glu Leu Met Met Thr Thr Ile 1220 1225 1230 Gly Ile Val Leu Leu Ser Gln Ser Thr Ile Pro Glu Thr Val Leu 1235 1240 1245 Glu Leu Thr Asp Ala Leu Ala Leu Gly Met Met Val Leu Lys Ile 1250 1255 1260 Val Arg Asn Met Glu Lys Tyr Gln Leu Ala Val Thr Ile Met Ala 1265 1270 1275 Ile Leu Cys Val Pro Asn Ala Val Ile Leu Gln Asn Ala Trp Lys 1280 1285 1290 Val Ser Cys Thr Ile Leu Ala Val Val Ser Val Ser Pro Leu Leu 1295 1300 1305 Leu Thr Ser Ser Gln Gln Lys Thr Asp Trp Ile Pro Leu Ala Leu 1310 1315 1320 Thr Ile Lys Gly Leu Asn Pro Thr Ala Ile Phe Leu Thr Thr Leu 1325 1330 1335 Ser Arg Thr Asn Lys Lys Arg Ser Trp Pro Leu Asn Glu Ala Ile 1340 1345 1350 Met Ala Val Gly Met Val Ser Ile Leu Ala Ser Ser Leu Leu Lys 1355 1360 1365 Asn Asp Ile Pro Met Thr Gly Pro Leu Val Ala Gly Gly Leu Leu 1370 1375 1380 Thr Val Cys Tyr Val Leu Thr Gly Arg Ser Ala Asp Leu Glu Leu 1385 1390 1395 Glu Arg Ala Thr Asp Val Lys Trp Glu Asp Gln Ala Glu Ile Ser 1400 1405 1410 Gly Ser Ser Pro Ile Leu Ser Ile Thr Ile Ser Glu Asp Gly Ser 1415 1420 1425 Met Ser Ile Lys Asn Glu Glu Glu Glu Gln Thr Leu Thr Ile Leu 1430 1435 1440 Ile Arg Thr Gly Leu Leu Val Ile Ser Gly Leu Phe Pro Ile Ser 1445 1450 1455 Ile Pro Val Thr Ala Ala Ala Trp Tyr Leu Trp Glu Val Lys Lys 1460 1465 1470 Gln Arg Ala Gly Val Leu Trp Asp Val Pro Ser Pro Pro Pro Met 1475 1480 1485 Gly Lys Ala Glu Leu Glu Asp Gly Ala Tyr Arg Ile Lys Gln Lys 1490 1495 1500 Gly Ile Leu Gly Tyr Ser Gln Ile Gly Ala Gly Val Tyr Lys Glu 1505 1510 1515 Gly Thr Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu 1520 1525 1530 Met His Lys Gly Lys Arg Ile Glu Pro Ser Trp Ala Asp Val Lys 1535 1540 1545 Lys Asp Leu Ile Ser Tyr Gly Gly Gly Trp Lys Leu Glu Gly Glu 1550 1555 1560 Trp Lys Glu Gly Glu Glu Val Gln Val Leu Ala Leu Glu Pro Gly 1565 1570 1575 Lys Asn Pro Arg Ala Val Gln Thr Lys Pro Gly Leu Phe Lys Thr 1580 1585 1590 Asn Thr Gly Thr Ile Gly Ala Val Ser Leu Asp Phe Ser Pro Gly 1595 1600 1605 Thr Ser Gly Ser Pro Ile Ile Asp Lys Lys Gly Lys Val Val Gly 1610 1615 1620 Leu Tyr Gly Asn Gly Val Val Thr Arg Ser Gly Ala Tyr Val Ser 1625 1630 1635 Ala Ile Ala Gln Thr Glu Lys Ser Ile Glu Asp Asn Pro Glu Ile 1640 1645 1650 Glu Asp Asp Ile Phe Arg Lys Arg Arg Leu Thr Ile Met Asp Leu 1655 1660 1665 His Pro Gly Ala Gly Lys Thr Lys Arg Tyr Leu Pro Ala Ile Val 1670 1675 1680 Arg Glu Ala Ile Lys Arg Gly Leu Arg Thr Leu Ile Leu Ala Pro 1685 1690 1695 Thr Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu 1700 1705 1710 Pro Ile Arg Tyr Gln Thr Pro Ala Ile Arg Ala Glu His Thr Gly 1715 1720 1725 Arg Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met Arg 1730 1735 1740 Leu Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Leu Ile Ile Met 1745 1750 1755 Asp Glu Ala His Phe Thr Asp Pro Ala Ser Ile Ala Ala Arg Gly 1760 1765 1770 Tyr Ile Ser Thr Arg Val Glu Met Gly Glu Ala Ala Gly Ile Phe 1775 1780 1785 Met Thr Ala Thr Pro Pro Gly Ser Arg Asp Pro Phe Pro Gln Ser 1790 1795 1800 Asn Ala Pro Ile Ile Asp Glu Glu Arg Glu Ile Pro Glu Arg Ser 1805 1810 1815 Trp Asn Ser Gly His Glu Trp Val Thr Asp Phe Lys Gly Lys Thr 1820 1825 1830 Val Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Ala 1835 1840 1845 Cys Leu Arg Lys Asn Gly Lys Lys Val Ile Gln Leu Ser Arg Lys 1850 1855 1860 Thr Phe Asp Ser Glu Tyr Val Lys Thr Arg Thr Asn Asp Trp Asp 1865 1870 1875 Phe Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys 1880 1885 1890 Ala Glu Arg Val Ile Asp Pro Arg Arg Cys Met Lys Pro Val Ile 1895 1900 1905 Leu Thr Asp Gly Glu Glu Arg Val Ile Leu Ala Gly Pro Met Pro 1910 1915 1920 Val Thr His Ser Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg 1925 1930 1935 Asn Pro Lys Asn Glu Asn Asp Gln Tyr Ile Tyr Met Gly Glu Pro 1940 1945 1950 Leu Glu Asn Asp Glu Asp Cys Ala His Trp Lys Glu Ala Lys Met 1955 1960 1965 Leu Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ser Met 1970 1975 1980 Phe Glu Pro Glu Arg Glu Lys Val Asp Ala Ile Asp Gly Glu Tyr 1985 1990 1995 Arg Leu Arg Gly Glu Ala Arg Lys Thr Phe Val Asp Leu Met Arg 2000 2005 2010 Arg Gly Asp Leu Pro Val Trp Leu Ala Tyr Lys Val Ala Ala Glu 2015 2020 2025 Gly Ile Asn Tyr Ala Asp Arg Arg Trp Cys Phe Asp Gly Ile Lys 2030 2035 2040 Asn Asn Gln Ile Leu Glu Glu Asn Val Glu Val Glu Ile Trp Thr 2045 2050 2055 Lys Glu Gly Glu Arg Lys Lys Leu Lys Pro Arg Trp Leu Asp Ala 2060 2065 2070 Arg Ile Tyr Ser Asp Pro Leu Ala Leu Lys Glu Phe Lys Glu Phe 2075 2080 2085 Ala Ala Gly Arg Lys Ser Leu Thr Leu Asn Leu Ile Thr Glu Met 2090 2095 2100 Gly Arg Leu Pro Thr Phe Met Thr Gln Lys Thr Arg Asp Ala Leu 2105 2110 2115 Asp Asn Leu Ala Val Leu His Thr Ala Glu Ala Gly Gly Arg Ala 2120 2125 2130 Tyr Asn His Ala Leu Ser Glu Leu Pro Glu Thr Leu Glu Thr Leu 2135 2140 2145 Leu Leu Leu Thr Leu Leu Ala Thr Val Thr Gly Gly Ile Phe Leu 2150 2155 2160 Phe Leu Met Ser Gly Arg Gly Ile Gly Lys Met Thr Leu Gly Met 2165 2170 2175 Cys Cys Ile Ile Thr Ala Ser Ile Leu Leu Trp Tyr Ala Gln Ile 2180 2185 2190 Gln Pro His Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe Leu 2195 2200 2205 Ile Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln 2210 2215 2220 Asp Asn Gln Leu Thr Tyr Val Val Ile Ala Ile Leu Thr Val Val 2225 2230 2235 Ala Ala Thr Met Ala Asn Glu Met Gly Phe Leu Glu Lys Thr Lys 2240 2245 2250 Lys Asp Leu Gly Leu Gly Ser Ile Ala Thr Gln Gln Pro Glu Ser 2255 2260 2265 Asn Ile Leu Asp Ile Asp Leu Arg Pro Ala Ser Ala Trp Thr Leu 2270 2275 2280 Tyr Ala Val Ala Thr Thr Phe Val Thr Pro Met Leu Arg His Ser 2285 2290 2295 Ile Glu Asn Ser Ser Val Asn Val Ser Leu Thr Ala Ile Ala Asn 2300 2305 2310 Gln Ala Thr Val Leu Met Gly Leu Gly Lys Gly Trp Pro Leu Ser 2315 2320 2325 Lys Met Asp Ile Gly Val Pro Leu Leu Ala Ile Gly Cys Tyr Ser 2330 2335 2340 Gln Val Asn Pro Ile Thr Leu Ile Ala Ala Leu Leu Leu Leu Val 2345 2350 2355 Ala His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr 2360 2365 2370 Arg Glu Ala Gln Lys Arg Ala Ala Ala Gly Ile Met Lys Asn Pro 2375 2380 2385 Thr Val Asp Gly Ile Thr Val Ile Asp Leu Asp Pro Ile Pro Tyr 2390 2395 2400 Asp Pro Lys Phe Glu Lys Gln Leu Gly Gln Val Met Leu Leu Ile 2405 2410 2415 Leu Cys Val Thr Gln Val Leu Met Met Arg Thr Thr Trp Ala Leu 2420 2425 2430 Cys Glu Ala Leu Thr Leu Ala Thr Gly Pro Ile Ser Thr Leu Trp 2435 2440 2445 Glu Gly Asn Pro Gly Arg Phe Trp Asn Thr Thr Ile Ala Val Ser 2450 2455 2460 Met Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu 2465 2470 2475 Leu Phe Ser Ile Met Lys Asn Thr Thr Asn Thr Arg Arg Gly Thr 2480 2485 2490 Gly Asn Ile Gly Glu Thr Leu Gly Glu Lys Trp Lys Ser Arg Leu 2495 2500 2505 Asn Ala Leu Gly Lys Ser Glu Phe Gln Ile Tyr Lys Lys Ser Gly 2510 2515 2520 Ile Gln Glu Val Asp Arg Thr Leu Ala Lys Glu Gly Ile Lys Arg 2525 2530 2535 Gly Glu Thr Asp His His Ala Val Ser Arg Gly Ser Ala Lys Leu 2540 2545 2550 Arg Trp Phe Val Glu Arg Asn Met Val Thr Pro Glu Gly Lys Val 2555 2560 2565 Val Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Cys Gly 2570 2575 2580 Gly Leu Lys Asn Val Arg Glu Val Lys Gly Leu Thr Lys Gly Gly 2585 2590 2595 Pro Gly His Glu Glu Pro Ile Pro Met Ser Thr Tyr Gly Trp Asn 2600 2605 2610 Leu Val Arg Leu Gln Ser Gly Val Asp Val Phe Phe Ile Pro Pro 2615 2620 2625 Glu Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Pro 2630 2635 2640 Asn Pro Thr Val Glu Ala Gly Arg Thr Leu Arg Val Leu Asn Leu 2645 2650 2655 Val Glu Asn Trp Leu Asn Asn Asn Thr Gln Phe Cys Ile Lys Val 2660 2665 2670 Leu Asn Pro Tyr Met Pro Ser Val Ile Glu Lys Met Glu Ala Leu 2675 2680 2685 Gln Arg Lys Tyr Gly Gly Ala Leu Val Arg Asn Pro Leu Ser Arg 2690 2695 2700 Asn Ser Thr His Glu Met Tyr Trp Val Ser Asn Ala Ser Gly Asn 2705 2710 2715 Ile Val Ser Ser Val Asn Met Ile Ser Arg Met Leu Ile Asn Arg 2720 2725 2730 Phe Thr Met Arg His Lys Lys Ala Thr Tyr Glu Pro Asp Val Asp 2735 2740 2745 Leu Gly Ser Gly Thr Arg Asn Ile Gly Val Glu Ser Glu Ile Pro 2750 2755 2760 Asn Leu Asp Ile Ile Gly Lys Arg Ile Glu Lys Ile Lys Gln Glu 2765 2770 2775 His Glu Thr Ser Trp His Tyr Asp Gln Asp His Pro Tyr Lys Thr 2780 2785 2790 Trp Ala Tyr His Gly Ser Tyr Glu Thr Lys Gln Thr Gly Ser Ala 2795 2800 2805 Ser Ser Met Val Asn Gly Val Val Arg Leu Leu Thr Lys Pro Trp 2810 2815 2820 Asp Val Leu Pro Thr Val Thr Gln Met Ala Met Thr Asp Thr Thr 2825 2830 2835 Pro Phe Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg 2840 2845 2850 Thr Gln Glu Pro Lys Glu Gly Thr Lys Lys Leu Met Arg Ile Thr 2855 2860 2865 Ala Glu Trp Leu Trp Lys Glu Leu Gly Lys Lys Lys Thr Pro Arg 2870 2875 2880 Met Cys Thr Arg Glu Glu Phe Thr Arg Lys Val Arg Ser Asn Ala 2885 2890 2895 Ala Leu Gly Ala Ile Phe Thr Asp Glu Asn Lys Trp Lys Ser Ala 2900 2905 2910 Arg Glu Ala Val Glu Asp Ser Arg Phe Trp Glu Leu Val Asp Lys 2915 2920 2925 Glu Arg Asn Leu His Leu Glu Gly Lys Cys Glu Thr Cys Val Tyr 2930 2935 2940 Asn Met Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Lys 2945 2950 2955 Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg 2960 2965 2970 Phe Leu Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp 2975 2980 2985 Phe Ser Arg Glu Asn Ser Leu Ser Gly Val Glu Gly Glu Gly Leu 2990 2995 3000 His Lys Leu Gly Tyr Ile Leu Arg Asp Val Ser Lys Lys Glu Gly 3005 3010 3015 Gly Ala Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile 3020 3025 3030 Thr Leu Glu Asp Leu Lys Asn Glu Glu Met Val Thr Asn His Met 3035 3040 3045 Glu Gly Glu His Lys Lys Leu Ala Glu Ala Ile Phe Lys Leu Thr 3050 3055 3060 Tyr Gln Asn Lys Val Val Arg Val Gln Arg Pro Thr Pro Arg Gly 3065 3070 3075 Thr Val Met Asp Ile Ile Ser Arg Arg Asp Gln Arg Gly Ser Gly 3080 3085 3090 Gln Val Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Ala 3095 3100 3105 Gln Leu Ile Arg Gln Met Glu Gly Glu Gly Val Phe Lys Asn Ile 3110 3115 3120 Gln His Leu Thr Val Thr Glu Glu Val Ala Val Gln Asn Trp Leu 3125 3130 3135 Ala Arg Val Gly Arg Glu Arg Leu Ser Arg Met Ala Ile Ser Gly 3140 3145 3150 Asp Asp Cys Val Val Lys Pro Leu Asp Asp Arg Phe Ala Asn Ala 3155 3160 3165 Leu Thr Ala Leu Asn Asp Met Gly Lys Ile Arg Lys Asp Ile Gln 3170 3175 3180 Gln Trp Glu Pro Ser Lys Gly Trp Asn Asp Trp Thr Gln Val Pro 3185 3190 3195 Phe Cys Ser His His Phe His Glu Leu Ile Met Lys Asp Gly Arg 3200 3205 3210 Val Leu Val Val Pro Cys Arg Asn Gln Asp Glu Leu Ile Gly Arg 3215 3220 3225 Ala Arg Ile Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala 3230 3235 3240 Cys Leu Gly Lys Ser Tyr Ala Gln Met Trp Ser Leu Met Tyr Phe 3245 3250 3255 His Arg Arg Asp Leu Arg Leu Ala Ala Asn Ala Ile Cys Ser Ala 3260 3265 3270 Val Pro Ser His Trp Val Pro Thr Ser Arg Thr Thr Trp Ser Ile 3275 3280 3285 His Ala Lys His Glu Trp Met Thr Thr Glu Asp Met Leu Thr Val 3290 3295 3300 Trp Asn Arg Val Trp Ile Arg Glu Asn Pro Trp Met Glu Asp Lys 3305 3310 3315 Thr Pro Val Glu Ser Trp Glu Glu Ile Pro Tyr Leu Gly Lys Arg 3320 3325 3330 Glu Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ser Arg Ala 3335 3340 3345 Thr Trp Ala Lys Asn Ile Gln Ala Ala Ile Asn Gln Val Arg Ser 3350 3355 3360 Leu Ile Gly Asn Glu Glu Tyr Thr Asp Tyr Met Pro Ser Met Lys 3365 3370 3375 Arg Phe Arg Arg Glu Glu Glu Glu Ala Gly Val Leu Trp 3380 3385 3390 <210> 3 <211> 3390 <212> PRT <213> Dengue virus type 3 <220> <221> VARIANT <222> (196)..(196) <223> Xaa stands for Gln or His <220> <221> VARIANT <222> (485)..(485) <223> Xaa stands for Met or Leu <220> <221> VARIANT <222> (671)..(671) <223> Xaa stands for Lys or Arg <220> <221> VARIANT <222> (1401)..(1401) <223> Xaa stands for Glu or Asp <220> <221> VARIANT <222> (2439)..(2439) <223> Xaa stands for Val or Ala <220> <221> VARIANT <222> (2894)..(2894) <223> Xaa stands for Thr or Ala <400> 3 Met Asn Asn Gln Arg Lys Lys Thr Gly Lys Pro Ser Ile Asn Met Leu 1 5 10 15 Lys Arg Val Arg Asn Arg Val Ser Thr Gly Ser Gln Leu Ala Lys Arg 20 25 30 Phe Ser Arg Gly Leu Leu Asn Gly Gln Gly Pro Met Lys Leu Val Met 35 40 45 Ala Phe Ile Ala Phe Leu Arg Phe Leu Ala Ile Pro Pro Thr Ala Gly 50 55 60 Val Leu Ala Arg Trp Gly Thr Phe Lys Lys Ser Gly Ala Ile Lys Val 65 70 75 80 Leu Lys Gly Phe Lys Lys Glu Ile Ser Asn Met Leu Ser Ile Ile Asn 85 90 95 Lys Arg Lys Lys Thr Ser Leu Cys Leu Met Met Met Leu Pro Ala Thr 100 105 110 Leu Ala Phe His Leu Thr Ser Arg Asp Gly Glu Pro Arg Met Ile Val 115 120 125 Gly Lys Asn Glu Arg Gly Lys Ser Leu Leu Phe Lys Thr Ala Ser Gly 130 135 140 Ile Asn Met Cys Thr Leu Ile Ala Met Asp Leu Gly Glu Met Cys Asp 145 150 155 160 Asp Thr Val Thr Tyr Lys Cys Pro His Ile Thr Glu Val Glu Pro Glu 165 170 175 Asp Ile Asp Cys Trp Cys Asn Leu Thr Ser Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Asn Xaa Ala Gly Glu His Arg Arg Asp Lys Arg Ser Val Ala 195 200 205 Ile Ala Pro His Val Gly Met Gly Leu Asp Thr Arg Thr Gln Thr Trp 210 215 220 Met Ser Ala Glu Gly Ala Trp Arg Gln Val Glu Lys Val Glu Thr Trp 225 230 235 240 Ala Leu Arg His Pro Gly Phe Thr Ile Leu Ala Leu Phe Leu Ala His 245 250 255 Tyr Ile Gly Thr Ser Leu Thr Gln Lys Val Val Ile Phe Ile Leu Leu 260 265 270 Met Leu Val Thr Pro Ser Met Thr Met Arg Cys Val Gly Val Gly Asn 275 280 285 Arg Asp Phe Val Glu Gly Leu Ser Gly Ala Thr Trp Val Asp Val Val 290 295 300 Leu Glu His Gly Gly Cys Val Thr Thr Met Ala Lys Asn Lys Pro Thr 305 310 315 320 Leu Asp Ile Glu Leu Gln Lys Thr Glu Ala Thr Gln Leu Ala Thr Leu 325 330 335 Arg Lys Leu Cys Ile Glu Gly Lys Ile Thr Asn Ile Thr Thr Asp Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Ala Ile Leu Pro Glu Glu Gln Asp Gln 355 360 365 Asn Tyr Val Cys Lys His Thr Tyr Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Gln 385 390 395 400 Cys Leu Glu Ser Ile Glu Gly Lys Val Val Gln His Glu Asn Leu Lys 405 410 415 Tyr Thr Val Ile Ile Thr Val His Thr Gly Asp Gln His Gln Val Gly 420 425 430 Asn Glu Thr Gln Gly Val Thr Ala Glu Ile Thr Pro Gln Ala Ser Thr 435 440 445 Ala Glu Ala Ile Leu Pro Glu Tyr Gly Thr Leu Gly Leu Glu Cys Ser 450 455 460 Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Ile Ser Leu Thr Met Lys 465 470 475 480 Asn Lys Ala Trp Xaa Val His Arg Gln Trp Phe Phe Asp Leu Pro Leu 485 490 495 Pro Trp Thr Ser Gly Ala Thr Ala Glu Thr Pro Thr Trp Asn Arg Lys 500 505 510 Glu Leu Leu Val Thr Phe Lys Asn Ala His Ala Lys Lys Gln Glu Val 515 520 525 Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu Thr Gly 530 535 540 Ala Thr Glu Ile Gln Thr Ser Gly Gly Thr Ser Ile Phe Ala Gly His 545 550 555 560 Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Glu Leu Lys Gly Met Ser 565 570 575 Tyr Ala Met Cys Leu Ser Ser Phe Val Leu Lys Lys Glu Val Ser Glu 580 585 590 Thr Gln His Gly Thr Ile Leu Ile Lys Val Glu Tyr Lys Gly Glu Asp 595 600 605 Ala Pro Cys Lys Ile Pro Phe Ser Thr Glu Asp Gly Gln Gly Lys Ala 610 615 620 His Asn Gly Arg Leu Ile Thr Ala Asn Pro Val Val Thr Lys Lys Glu 625 630 635 640 Glu Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser Asn Ile 645 650 655 Val Ile Gly Ile Gly Asp Lys Ala Leu Lys Ile Asn Trp Tyr Xaa Lys 660 665 670 Gly Ser Ser Ile Gly Lys Met Phe Glu Ala Thr Ala Arg Gly Ala Arg 675 680 685 Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly 690 695 700 Gly Val Leu Asn Ser Leu Gly Lys Met Val His Gln Ile Phe Gly Ser 705 710 715 720 Ala Tyr Thr Ala Leu Phe Gly Gly Val Ser Trp Met Met Lys Ile Gly 725 730 735 Ile Gly Val Leu Leu Thr Trp Ile Gly Leu Asn Ser Lys Asn Thr Ser 740 745 750 Met Ser Phe Ser Cys Ile Ala Ile Gly Ile Ile Thr Leu Tyr Leu Gly 755 760 765 Ala Val Val Gln Ala Asp Met Gly Cys Val Ile Asn Trp Lys Gly Lys 770 775 780 Glu Leu Lys Cys Gly Ser Gly Ile Phe Val Thr Asn Glu Val His Thr 785 790 795 800 Trp Thr Glu Gln Tyr Lys Phe Gln Ala Asp Ser Pro Lys Arg Leu Ala 805 810 815 Thr Ala Ile Ala Gly Ala Trp Glu Asn Gly Val Cys Gly Ile Arg Ser 820 825 830 Thr Thr Arg Met Glu Asn Leu Leu Trp Lys Gln Ile Ala Asn Glu Leu 835 840 845 Asn Tyr Ile Leu Trp Glu Asn Asn Ile Lys Leu Thr Val Val Val Gly 850 855 860 Asp Ile Thr Gly Val Leu Glu Gln Gly Lys Arg Thr Leu Thr Pro Gln 865 870 875 880 Pro Met Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala Lys Ile 885 890 895 Val Thr Ala Glu Thr Gln Asn Ser Ser Phe Ile Ile Asp Gly Pro Ser 900 905 910 Thr Pro Glu Cys Pro Ser Ala Ser Arg Ala Trp Asn Val Trp Glu Val 915 920 925 Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu Lys Leu 930 935 940 Arg Glu Val Tyr Thr Gln Leu Cys Asp His Arg Leu Met Ser Ala Ala 945 950 955 960 Val Lys Asp Glu Arg Ala Val His Ala Asp Met Gly Tyr Trp Ile Glu 965 970 975 Ser Gln Lys Asn Gly Ser Trp Lys Leu Glu Lys Ala Ser Phe Ile Glu 980 985 990 Val Lys Thr Cys Thr Trp Pro Lys Ser His Thr Leu Trp Ser Asn Gly 995 1000 1005 Val Leu Glu Ser Asp Met Ile Ile Pro Lys Ser Leu Ala Gly Pro 1010 1015 1020 Ile Ser Gln His Asn His Arg Pro Gly Tyr His Thr Gln Thr Ala 1025 1030 1035 Gly Pro Trp His Leu Gly Lys Leu Glu Leu Asp Phe Asn Tyr Cys 1040 1045 1050 Glu Gly Thr Thr Val Val Ile Ser Glu Asn Cys Gly Thr Arg Gly 1055 1060 1065 Pro Ser Leu Arg Thr Thr Thr Val Ser Gly Lys Leu Ile His Glu 1070 1075 1080 Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Tyr Met Gly 1085 1090 1095 Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Ile Asn Glu 1100 1105 1110 Lys Glu Glu Asn Met Val Lys Ser Leu Ala Ser Ala Gly Ser Gly 1115 1120 1125 Lys Val Asp Asn Phe Thr Met Gly Val Leu Cys Leu Ala Ile Leu 1130 1135 1140 Phe Glu Glu Val Met Arg Gly Lys Phe Gly Lys Lys His Met Ile 1145 1150 1155 Ala Gly Val Leu Phe Thr Phe Val Leu Leu Leu Ser Gly Gln Ile 1160 1165 1170 Thr Trp Arg Asp Met Ala His Thr Leu Ile Met Ile Gly Ser Asn 1175 1180 1185 Ala Ser Asp Arg Met Gly Met Gly Val Thr Tyr Leu Ala Leu Ile 1190 1195 1200 Ala Thr Phe Lys Ile Gln Pro Phe Leu Ala Leu Gly Phe Phe Leu 1205 1210 1215 Arg Lys Leu Thr Ser Arg Glu Asn Leu Leu Leu Gly Val Gly Leu 1220 1225 1230 Ala Met Ala Ala Thr Leu Arg Leu Pro Glu Asp Ile Glu Gln Met 1235 1240 1245 Ala Asn Gly Ile Ala Leu Gly Leu Met Ala Leu Lys Leu Ile Thr 1250 1255 1260 Gln Phe Glu Thr Tyr Gln Leu Trp Thr Ala Leu Val Ser Leu Thr 1265 1270 1275 Cys Ser Asn Thr Ile Phe Thr Leu Thr Val Ala Trp Arg Thr Ala 1280 1285 1290 Thr Leu Ile Leu Ala Gly Ile Ser Leu Leu Pro Val Cys Gln Ser 1295 1300 1305 Ser Ser Met Arg Lys Thr Asp Trp Leu Pro Met Thr Val Ala Ala 1310 1315 1320 Met Gly Ala Gln Pro Leu Pro Leu Phe Ile Phe Ser Leu Lys Asp 1325 1330 1335 Thr Leu Lys Arg Arg Ser Trp Pro Leu Asn Glu Gly Val Met Ala 1340 1345 1350 Val Gly Leu Val Ser Ile Leu Ala Ser Ser Leu Leu Arg Asn Asp 1355 1360 1365 Val Pro Met Ala Gly Pro Leu Val Ala Gly Gly Leu Leu Ile Ala 1370 1375 1380 Cys Tyr Val Ile Thr Gly Thr Ser Ala Asp Leu Thr Val Glu Lys 1385 1390 1395 Ala Ala Xaa Val Thr Trp Glu Glu Glu Ala Glu Gln Thr Gly Val 1400 1405 1410 Ser His Asn Leu Met Val Thr Val Asp Asp Asp Gly Thr Met Arg 1415 1420 1425 Ile Lys Asp Asp Glu Thr Glu Asn Ile Leu Thr Val Leu Leu Lys 1430 1435 1440 Thr Ala Leu Leu Ile Val Ser Gly Ile Phe Pro Tyr Ser Ile Pro 1445 1450 1455 Ala Thr Leu Leu Val Trp His Thr Trp Gln Lys Gln Thr Gln Arg 1460 1465 1470 Ser Gly Val Leu Trp Asp Val Pro Ser Pro Pro Glu Thr Gln Lys 1475 1480 1485 Ala Glu Leu Glu Glu Gly Val Tyr Arg Ile Lys Gln Gln Gly Ile 1490 1495 1500 Phe Gly Lys Thr Gln Val Gly Val Gly Val Gln Lys Glu Gly Val 1505 1510 1515 Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu Thr His 1520 1525 1530 Asn Gly Lys Arg Leu Glu Pro Asn Trp Ala Ser Val Lys Lys Asp 1535 1540 1545 Leu Ile Ser Tyr Gly Gly Gly Trp Arg Leu Ser Ala Gln Trp Gln 1550 1555 1560 Lys Gly Glu Glu Val Gln Val Ile Ala Val Glu Pro Gly Lys Asn 1565 1570 1575 Pro Lys Asn Phe Gln Thr Met Pro Gly Ile Phe Gln Thr Thr Thr 1580 1585 1590 Gly Glu Ile Gly Ala Ile Ala Leu Asp Phe Lys Pro Gly Thr Ser 1595 1600 1605 Gly Ser Pro Ile Ile Asn Arg Glu Gly Lys Val Val Gly Leu Tyr 1610 1615 1620 Gly Asn Gly Val Val Thr Lys Asn Gly Gly Tyr Val Ser Gly Ile 1625 1630 1635 Ala Gln Thr Asn Ala Glu Pro Asp Gly Pro Thr Pro Glu Leu Glu 1640 1645 1650 Glu Glu Met Phe Lys Lys Arg Asn Leu Thr Ile Met Asp Leu His 1655 1660 1665 Pro Gly Ser Gly Lys Thr Arg Lys Tyr Leu Pro Ala Ile Val Arg 1670 1675 1680 Glu Ala Ile Lys Arg Arg Leu Arg Thr Leu Ile Leu Ala Pro Thr 1685 1690 1695 Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Lys Gly Leu Pro 1700 1705 1710 Ile Arg Tyr Gln Thr Thr Ala Thr Lys Ser Glu His Thr Gly Arg 1715 1720 1725 Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met Arg Leu 1730 1735 1740 Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Leu Ile Ile Met Asp 1745 1750 1755 Glu Ala His Phe Thr Asp Pro Ala Ser Ile Ala Ala Arg Gly Tyr 1760 1765 1770 Ile Ser Thr Arg Val Gly Met Gly Glu Ala Ala Ala Ile Phe Met 1775 1780 1785 Thr Ala Thr Pro Pro Gly Thr Ala Asp Ala Phe Pro Gln Ser Asn 1790 1795 1800 Ala Pro Ile Gln Asp Glu Glu Arg Asp Ile Pro Glu Arg Ser Trp 1805 1810 1815 Asn Ser Gly Asn Glu Trp Ile Thr Asp Phe Val Gly Lys Thr Val 1820 1825 1830 Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Asn Cys 1835 1840 1845 Leu Arg Lys Asn Gly Lys Lys Val Ile Gln Leu Ser Arg Lys Thr 1850 1855 1860 Phe Asp Thr Glu Tyr Gln Lys Thr Lys Leu Asn Asp Trp Asp Phe 1865 1870 1875 Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys Ala 1880 1885 1890 Asp Arg Val Ile Asp Pro Arg Arg Cys Leu Lys Pro Val Ile Leu 1895 1900 1905 Thr Asp Gly Pro Glu Arg Val Ile Leu Ala Gly Pro Met Pro Val 1910 1915 1920 Thr Val Ala Ser Ala Ala Gln Arg Arg Gly Arg Val Gly Arg Asn 1925 1930 1935 Pro Gln Lys Glu Asn Asp Gln Tyr Ile Phe Met Gly Gln Pro Leu 1940 1945 1950 Asn Asn Asp Glu Asp His Ala His Trp Thr Glu Ala Lys Met Leu 1955 1960 1965 Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ala Leu Phe 1970 1975 1980 Glu Pro Glu Arg Glu Lys Ser Ala Ala Ile Asp Gly Glu Tyr Arg 1985 1990 1995 Leu Lys Gly Glu Ser Arg Lys Thr Phe Val Glu Leu Met Arg Arg 2000 2005 2010 Gly Asp Leu Pro Val Trp Leu Ala His Lys Val Ala Ser Glu Gly 2015 2020 2025 Ile Lys Tyr Thr Asp Arg Lys Trp Cys Phe Asp Gly Glu Arg Asn 2030 2035 2040 Asn Gln Ile Leu Glu Glu Asn Met Asp Val Glu Ile Trp Thr Lys 2045 2050 2055 Glu Gly Glu Lys Lys Lys Leu Arg Pro Arg Trp Leu Asp Ala Arg 2060 2065 2070 Thr Tyr Ser Asp Pro Leu Ala Leu Lys Glu Phe Lys Asp Phe Ala 2075 2080 2085 Ala Gly Arg Lys Ser Ile Ala Leu Asp Leu Val Thr Glu Ile Gly 2090 2095 2100 Arg Val Pro Ser His Leu Ala His Arg Thr Arg Asn Ala Leu Asp 2105 2110 2115 Asn Leu Val Met Leu His Thr Ser Glu His Gly Gly Arg Ala Tyr 2120 2125 2130 Arg His Ala Val Glu Glu Leu Pro Glu Thr Met Glu Thr Leu Leu 2135 2140 2145 Leu Leu Gly Leu Met Ile Leu Leu Thr Gly Gly Ala Met Leu Phe 2150 2155 2160 Leu Ile Ser Gly Lys Gly Ile Gly Lys Thr Ser Ile Gly Leu Ile 2165 2170 2175 Cys Val Ile Ala Ser Ser Gly Met Leu Trp Met Ala Asp Val Pro 2180 2185 2190 Leu Gln Trp Ile Ala Ser Ala Ile Val Leu Glu Phe Phe Met Met 2195 2200 2205 Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln Asp 2210 2215 2220 Asn Gln Leu Ala Tyr Val Val Ile Gly Ile Leu Thr Leu Ala Ala 2225 2230 2235 Ile Val Ala Ala Asn Glu Met Gly Leu Leu Glu Thr Thr Lys Arg 2240 2245 2250 Asp Leu Gly Met Ser Lys Glu Pro Gly Val Val Ser Pro Thr Ser 2255 2260 2265 Tyr Leu Asp Val Asp Leu His Pro Ala Ser Ala Trp Thr Leu Tyr 2270 2275 2280 Ala Val Ala Thr Thr Val Ile Thr Pro Met Leu Arg His Thr Ile 2285 2290 2295 Glu Asn Ser Thr Ala Asn Val Ser Leu Ala Ala Ile Ala Asn Gln 2300 2305 2310 Ala Val Val Leu Met Gly Leu Asp Lys Gly Trp Pro Ile Ser Lys 2315 2320 2325 Met Asp Leu Gly Val Pro Leu Leu Ala Leu Gly Cys Tyr Ser Gln 2330 2335 2340 Val Asn Pro Leu Thr Leu Ala Ala Ala Val Leu Leu Leu Val Thr 2345 2350 2355 His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr Arg 2360 2365 2370 Glu Ala Gln Lys Arg Thr Ala Ala Gly Ile Met Lys Asn Pro Thr 2375 2380 2385 Val Asp Gly Ile Met Thr Ile Asp Leu Asp Pro Val Ile Tyr Asp 2390 2395 2400 Ser Lys Phe Glu Lys Gln Leu Gly Gln Val Met Leu Leu Val Leu 2405 2410 2415 Cys Ala Val Gln Leu Leu Leu Met Arg Thr Ser Trp Ala Leu Cys 2420 2425 2430 Glu Val Leu Thr Leu Xaa Thr Gly Pro Ile Thr Thr Leu Trp Glu 2435 2440 2445 Gly Ser Pro Gly Lys Phe Trp Asn Thr Thr Ile Ala Val Ser Met 2450 2455 2460 Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu Ala 2465 2470 2475 Phe Ser Ile Met Lys Ser Val Gly Thr Gly Lys Arg Gly Thr Gly 2480 2485 2490 Ser Gln Gly Glu Thr Leu Gly Glu Lys Trp Lys Lys Lys Leu Asn 2495 2500 2505 Gln Leu Ser Arg Lys Glu Phe Asp Leu Tyr Lys Lys Ser Gly Ile 2510 2515 2520 Thr Glu Val Asp Arg Thr Glu Ala Lys Glu Gly Leu Lys Arg Gly 2525 2530 2535 Glu Ile Thr His His Ala Val Ser Arg Gly Ser Ala Lys Leu Gln 2540 2545 2550 Trp Phe Val Glu Arg Asn Met Val Ile Pro Glu Gly Arg Val Ile 2555 2560 2565 Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Cys Ala Gly 2570 2575 2580 Leu Lys Lys Val Thr Glu Val Arg Gly Tyr Thr Lys Gly Gly Pro 2585 2590 2595 Gly His Glu Glu Pro Val Pro Met Ser Thr Tyr Gly Trp Asn Ile 2600 2605 2610 Val Lys Leu Met Ser Gly Lys Asp Val Phe Tyr Leu Pro Pro Glu 2615 2620 2625 Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Pro Ser 2630 2635 2640 Pro Thr Val Glu Glu Ser Arg Thr Ile Arg Val Leu Lys Met Val 2645 2650 2655 Glu Pro Trp Leu Lys Asn Asn Gln Phe Cys Ile Lys Val Leu Asn 2660 2665 2670 Pro Tyr Met Pro Thr Val Ile Glu His Leu Glu Arg Leu Gln Arg 2675 2680 2685 Lys His Gly Gly Met Leu Val Arg Asn Pro Leu Ser Arg Asn Ser 2690 2695 2700 Thr His Glu Met Tyr Trp Ile Ser Asn Gly Thr Gly Asn Ile Val 2705 2710 2715 Ser Ser Val Asn Met Val Ser Arg Leu Leu Leu Asn Arg Phe Thr 2720 2725 2730 Met Thr His Arg Arg Pro Thr Ile Glu Lys Asp Val Asp Leu Gly 2735 2740 2745 Ala Gly Thr Arg His Val Asn Ala Glu Pro Glu Thr Pro Asn Met 2750 2755 2760 Asp Val Ile Gly Glu Arg Ile Lys Arg Ile Lys Glu Glu His Ser 2765 2770 2775 Ser Thr Trp His Tyr Asp Asp Glu Asn Pro Tyr Lys Thr Trp Ala 2780 2785 2790 Tyr His Gly Ser Tyr Glu Val Lys Ala Thr Gly Ser Ala Ser Ser 2795 2800 2805 Met Ile Asn Gly Val Val Lys Leu Leu Thr Lys Pro Trp Asp Val 2810 2815 2820 Val Pro Met Val Thr Gln Met Ala Met Thr Asp Thr Thr Pro Phe 2825 2830 2835 Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg Thr Pro 2840 2845 2850 Arg Pro Met Pro Gly Thr Arg Lys Val Met Glu Ile Thr Ala Glu 2855 2860 2865 Trp Leu Trp Arg Thr Leu Gly Arg Asn Lys Arg Pro Arg Leu Cys 2870 2875 2880 Thr Arg Glu Glu Phe Thr Lys Lys Val Arg Xaa Asn Ala Ala Met 2885 2890 2895 Gly Ala Val Phe Thr Glu Glu Asn Gln Trp Asp Ser Ala Arg Ala 2900 2905 2910 Ala Val Glu Asp Glu Glu Phe Trp Lys Leu Val Asp Arg Glu Arg 2915 2920 2925 Glu Leu His Lys Leu Gly Lys Cys Gly Ser Cys Val Tyr Asn Met 2930 2935 2940 Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Lys Ala Lys 2945 2950 2955 Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg Tyr Leu 2960 2965 2970 Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp Phe Ser 2975 2980 2985 Arg Glu Asn Ser Tyr Ser Gly Val Glu Gly Glu Gly Leu His Lys 2990 2995 3000 Leu Gly Tyr Ile Leu Arg Asp Ile Ser Lys Ile Pro Gly Gly Ala 3005 3010 3015 Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile Thr Glu 3020 3025 3030 Asp Asp Leu His Asn Glu Glu Lys Ile Thr Gln Gln Met Asp Pro 3035 3040 3045 Glu His Arg Gln Leu Ala Asn Ala Ile Phe Lys Leu Thr Tyr Gln 3050 3055 3060 Asn Lys Val Val Lys Val Gln Arg Pro Thr Pro Lys Gly Thr Val 3065 3070 3075 Met Asp Ile Ile Ser Arg Lys Asp Gln Arg Gly Ser Gly Gln Val 3080 3085 3090 Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Ala Gln Leu 3095 3100 3105 Ile Arg Gln Met Glu Gly Glu Gly Val Leu Ser Lys Ala Asp Leu 3110 3115 3120 Glu Asn Pro His Pro Leu Glu Lys Lys Ile Thr Gln Trp Leu Glu 3125 3130 3135 Thr Lys Gly Val Glu Arg Leu Lys Arg Met Ala Ile Ser Gly Asp 3140 3145 3150 Asp Cys Val Val Lys Pro Ile Asp Asp Arg Phe Ala Asn Ala Leu 3155 3160 3165 Leu Ala Leu Asn Asp Met Gly Lys Val Arg Lys Asp Ile Pro Gln 3170 3175 3180 Trp Gln Pro Ser Lys Gly Trp His Asp Trp Gln Gln Val Pro Phe 3185 3190 3195 Cys Ser His His Phe His Glu Leu Ile Met Lys Asp Gly Arg Lys 3200 3205 3210 Leu Val Val Pro Cys Arg Pro Gln Asp Glu Leu Ile Gly Arg Ala 3215 3220 3225 Arg Ile Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala Cys 3230 3235 3240 Leu Gly Lys Ala Tyr Ala Gln Met Trp Ala Leu Met Tyr Phe His 3245 3250 3255 Arg Arg Asp Leu Arg Leu Ala Ser Asn Ala Ile Cys Ser Ala Val 3260 3265 3270 Pro Val His Trp Val Pro Thr Ser Arg Thr Thr Trp Ser Ile His 3275 3280 3285 Ala His His Gln Trp Met Thr Thr Glu Asp Met Leu Thr Val Trp 3290 3295 3300 Asn Arg Val Trp Ile Glu Asp Asn Pro Trp Met Glu Asp Lys Thr 3305 3310 3315 Pro Val Thr Thr Trp Glu Asp Val Pro Tyr Leu Gly Lys Arg Glu 3320 3325 3330 Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ser Arg Ala Thr 3335 3340 3345 Trp Ala Gln Asn Ile Leu Thr Ala Ile Gln Gln Val Arg Ser Leu 3350 3355 3360 Ile Gly Asn Glu Glu Phe Leu Asp Tyr Met Pro Ser Met Lys Arg 3365 3370 3375 Phe Arg Lys Glu Glu Glu Ser Glu Gly Ala Ile Trp 3380 3385 3390 <210> 4 <211> 3387 <212> PRT <213> Dengue virus type 4 <220> <221> VARIANT <222> (1429)..(1429) <223> Xaa stands for Ser or Phe <220> <221> VARIANT <222> (2217)..(2217) <223> Xaa stands for Lys or Arg <220> <221> VARIANT <222> (2255)..(2255) <223> Xaa stands for Phe or Leu <220> <221> VARIANT <222> (2344)..(2344) <223> Xaa stands for Thr or Ile <220> <221> VARIANT <222> (2354)..(2354) <223> Xaa stands for Phe or Leu <220> <221> VARIANT <222> (2877)..(2877) <223> Xaa stands for Asn or Lys <400> 4 Met Asn Gln Arg Lys Lys Val Val Arg Pro Pro Phe Asn Met Leu Lys 1 5 10 15 Arg Glu Arg Asn Arg Val Ser Thr Pro Gln Gly Leu Val Lys Arg Phe 20 25 30 Ser Thr Gly Leu Phe Ser Gly Lys Gly Pro Leu Arg Met Val Leu Ala 35 40 45 Phe Ile Thr Phe Leu Arg Val Leu Ser Ile Pro Pro Thr Ala Gly Ile 50 55 60 Leu Lys Arg Trp Gly Gln Leu Lys Lys Asn Lys Ala Ile Arg Ile Leu 65 70 75 80 Ile Gly Phe Arg Lys Glu Ile Gly Arg Met Leu Asn Ile Leu Asn Gly 85 90 95 Arg Lys Arg Ser Thr Ile Thr Leu Leu Cys Leu Ile Pro Thr Val Met 100 105 110 Ala Phe His Leu Ser Thr Arg Asp Gly Glu Pro Leu Met Ile Val Ala 115 120 125 Lys His Glu Arg Gly Arg Pro Leu Leu Phe Lys Thr Thr Glu Gly Ile 130 135 140 Asn Lys Cys Thr Leu Ile Ala Met Asp Leu Gly Glu Met Cys Glu Asp 145 150 155 160 Thr Val Thr Tyr Lys Cys Pro Leu Leu Val Asn Thr Glu Pro Glu Asp 165 170 175 Ile Asp Cys Trp Cys Asn Leu Thr Ser Thr Trp Val Met Tyr Gly Thr 180 185 190 Cys Thr Gln Ser Gly Glu Arg Arg Arg Glu Lys Arg Ser Val Ala Leu 195 200 205 Thr Pro His Ser Gly Met Gly Leu Glu Thr Arg Ala Glu Thr Trp Met 210 215 220 Ser Ser Glu Gly Ala Trp Lys His Ala Gln Arg Val Glu Ser Trp Ile 225 230 235 240 Leu Arg Asn Pro Gly Phe Ala Leu Leu Ala Gly Phe Met Ala Tyr Met 245 250 255 Ile Gly Gln Thr Gly Ile Gln Arg Thr Val Phe Phe Val Leu Met Met 260 265 270 Leu Val Ala Pro Ser Tyr Gly Met Arg Cys Val Gly Val Gly Asn Arg 275 280 285 Asp Phe Val Glu Gly Val Ser Gly Gly Ala Trp Val Asp Leu Val Leu 290 295 300 Glu His Gly Gly Cys Val Thr Thr Met Ala Gln Gly Lys Pro Thr Leu 305 310 315 320 Asp Phe Glu Leu Thr Lys Thr Thr Ala Lys Glu Val Ala Leu Leu Arg 325 330 335 Thr Tyr Cys Ile Glu Ala Ser Ile Ser Asn Ile Thr Thr Ala Thr Arg 340 345 350 Cys Pro Thr Gln Gly Glu Pro Tyr Leu Lys Glu Glu Gln Asp Gln Gln 355 360 365 Tyr Ile Cys Arg Arg Asp Val Val Asp Arg Gly Trp Gly Asn Gly Cys 370 375 380 Gly Leu Phe Gly Lys Gly Gly Val Val Thr Cys Ala Lys Phe Ser Cys 385 390 395 400 Ser Gly Lys Ile Thr Gly Asn Leu Val Gln Ile Glu Asn Leu Glu Tyr 405 410 415 Thr Val Val Val Thr Val His Asn Gly Asp Thr His Ala Val Gly Asn 420 425 430 Asp Thr Ser Asn His Gly Val Thr Ala Thr Ile Thr Pro Arg Ser Pro 435 440 445 Ser Val Glu Val Lys Leu Pro Asp Tyr Gly Glu Leu Thr Leu Asp Cys 450 455 460 Glu Pro Arg Ser Gly Ile Asp Phe Asn Glu Met Ile Leu Met Lys Met 465 470 475 480 Lys Lys Lys Thr Trp Leu Val His Lys Gln Trp Phe Leu Asp Leu Pro 485 490 495 Leu Pro Trp Thr Ala Gly Ala Asp Thr Ser Glu Val His Trp Asn Tyr 500 505 510 Lys Glu Arg Met Val Thr Phe Lys Val Pro His Ala Lys Arg Gln Asp 515 520 525 Val Thr Val Leu Gly Ser Gln Glu Gly Ala Met His Ser Ala Leu Ala 530 535 540 Gly Ala Thr Glu Val Asp Ser Gly Asp Gly Asn His Met Phe Ala Gly 545 550 555 560 His Leu Lys Cys Lys Val Arg Met Glu Lys Leu Arg Ile Lys Gly Met 565 570 575 Ser Tyr Thr Met Cys Ser Gly Lys Phe Ser Ile Asp Lys Glu Met Ala 580 585 590 Glu Thr Gln His Gly Thr Thr Val Val Lys Val Lys Tyr Glu Gly Ala 595 600 605 Gly Ala Pro Cys Lys Val Pro Ile Glu Ile Arg Asp Val Asn Lys Glu 610 615 620 Lys Val Val Gly Arg Ile Ile Ser Ser Thr Pro Leu Ala Glu Asn Thr 625 630 635 640 Asn Ser Ala Thr Asn Ile Glu Leu Glu Pro Pro Phe Gly Asp Ser Tyr 645 650 655 Ile Val Ile Gly Val Gly Asn Ser Ala Leu Thr Leu His Trp Phe Arg 660 665 670 Lys Gly Ser Ser Ile Gly Lys Met Phe Glu Ser Thr Tyr Arg Gly Ala 675 680 685 Lys Arg Met Ala Ile Leu Gly Glu Thr Ala Trp Asp Phe Gly Ser Val 690 695 700 Gly Gly Leu Phe Thr Ser Leu Gly Lys Ala Val His Gln Val Phe Gly 705 710 715 720 Ser Val Tyr Thr Thr Met Phe Gly Gly Val Ser Trp Met Ile Arg Ile 725 730 735 Leu Ile Gly Phe Leu Val Leu Trp Ile Gly Thr Asn Ser Arg Asn Thr 740 745 750 Ser Met Ala Met Thr Cys Ile Ala Val Gly Gly Ile Thr Leu Phe Leu 755 760 765 Gly Phe Thr Val Gln Ala Asp Met Gly Cys Val Val Ser Trp Ser Gly 770 775 780 Lys Glu Leu Lys Cys Gly Ser Gly Ile Phe Val Val Asp Asn Val His 785 790 795 800 Thr Trp Thr Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ala Arg Leu 805 810 815 Ala Ser Ala Ile Leu Asn Ala His Lys Asp Gly Val Cys Gly Ile Arg 820 825 830 Ser Thr Thr Arg Leu Glu Asn Val Met Trp Lys Gln Ile Thr Asn Glu 835 840 845 Leu Asn Tyr Val Leu Trp Glu Gly Gly His Asp Leu Thr Val Val Ala 850 855 860 Gly Asp Val Lys Gly Val Leu Thr Lys Gly Lys Arg Ala Leu Thr Pro 865 870 875 880 Pro Val Asn Asp Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala Lys 885 890 895 Ile Phe Thr Pro Glu Ala Arg Asn Ser Thr Phe Leu Ile Asp Gly Pro 900 905 910 Asp Thr Ser Glu Cys Pro Asn Glu Arg Arg Ala Trp Asn Phe Leu Glu 915 920 925 Val Glu Asp Tyr Gly Phe Gly Met Phe Thr Thr Asn Ile Trp Met Lys 930 935 940 Phe Arg Glu Gly Ser Ser Glu Val Cys Asp His Arg Leu Met Ser Ala 945 950 955 960 Ala Ile Lys Asp Gln Lys Ala Val His Ala Asp Met Gly Tyr Trp Ile 965 970 975 Glu Ser Ser Lys Asn Gln Thr Trp Gln Ile Glu Lys Ala Ser Leu Ile 980 985 990 Glu Val Lys Thr Cys Leu Trp Pro Lys Thr His Thr Leu Trp Ser Asn 995 1000 1005 Gly Val Leu Glu Ser Gln Met Leu Ile Pro Lys Ser Tyr Ala Gly 1010 1015 1020 Pro Phe Ser Gln His Asn Tyr Arg Gln Gly Tyr Ala Thr Gln Thr 1025 1030 1035 Val Gly Pro Trp His Leu Gly Lys Leu Glu Ile Asp Phe Gly Glu 1040 1045 1050 Cys Pro Gly Thr Thr Val Ala Ile Gln Glu Asp Cys Asp His Arg 1055 1060 1065 Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys Leu Val Thr 1070 1075 1080 Gln Trp Cys Cys Arg Ser Cys Thr Met Pro Pro Leu Arg Phe Leu 1085 1090 1095 Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu Ser 1100 1105 1110 Glu Lys Glu Glu Asn Met Val Lys Ser Gln Val Thr Ala Gly Gln 1115 1120 1125 Gly Thr Ser Glu Thr Phe Ser Met Gly Leu Leu Cys Leu Thr Leu 1130 1135 1140 Phe Val Glu Glu Cys Leu Arg Arg Arg Val Thr Arg Lys His Met 1145 1150 1155 Ile Leu Val Val Val Ile Thr Leu Cys Ala Ile Ile Leu Gly Gly 1160 1165 1170 Leu Thr Trp Met Asp Leu Leu Arg Ala Leu Ile Met Leu Gly Asp 1175 1180 1185 Thr Met Ser Gly Arg Ile Gly Gly Gln Ile His Leu Ala Ile Met 1190 1195 1200 Ala Val Phe Lys Met Ser Pro Gly Tyr Val Leu Gly Val Phe Leu 1205 1210 1215 Arg Lys Leu Thr Ser Arg Glu Thr Ala Leu Met Val Ile Gly Met 1220 1225 1230 Ala Met Thr Thr Val Leu Ser Ile Pro His Asp Leu Met Glu Leu 1235 1240 1245 Ile Asp Gly Ile Ser Leu Gly Leu Ile Leu Leu Lys Ile Val Thr 1250 1255 1260 His Phe Asp Asn Thr Gln Val Gly Thr Leu Ala Leu Ser Leu Thr 1265 1270 1275 Phe Ile Arg Ser Thr Met Pro Leu Val Met Ala Trp Arg Thr Ile 1280 1285 1290 Met Ala Val Leu Phe Val Val Thr Leu Ile Pro Leu Cys Arg Thr 1295 1300 1305 Ser Cys Leu Gln Lys Gln Ser His Trp Val Glu Ile Thr Ala Leu 1310 1315 1320 Ile Leu Gly Ala Gln Ala Leu Pro Val Tyr Leu Met Thr Leu Met 1325 1330 1335 Lys Gly Ala Ser Arg Arg Ser Trp Pro Leu Asn Glu Gly Ile Met 1340 1345 1350 Ala Val Gly Leu Val Ser Leu Leu Gly Ser Ala Leu Leu Lys Asn 1355 1360 1365 Asp Val Pro Leu Ala Gly Pro Met Val Ala Gly Gly Leu Leu Leu 1370 1375 1380 Ala Ala Tyr Val Met Ser Gly Ser Ser Ala Asp Leu Ser Leu Glu 1385 1390 1395 Lys Ala Ala Asn Val Gln Trp Asp Glu Met Ala Asp Ile Thr Gly 1400 1405 1410 Ser Ser Pro Ile Ile Glu Val Lys Gln Asp Glu Asp Gly Ser Phe 1415 1420 1425 Xaa Ile Arg Asp Val Glu Glu Thr Asn Met Ile Thr Leu Leu Val 1430 1435 1440 Lys Leu Ala Leu Ile Thr Val Ser Gly Leu Tyr Pro Leu Ala Ile 1445 1450 1455 Pro Val Thr Met Thr Leu Trp Tyr Met Trp Gln Val Lys Thr Gln 1460 1465 1470 Arg Ser Gly Ala Leu Trp Asp Val Pro Ser Pro Ala Ala Thr Gln 1475 1480 1485 Lys Ala Ala Leu Ser Glu Gly Val Tyr Arg Ile Met Gln Arg Gly 1490 1495 1500 Leu Phe Gly Lys Thr Gln Val Gly Val Gly Ile His Met Glu Gly 1505 1510 1515 Val Phe His Thr Met Trp His Val Thr Arg Gly Ser Val Ile Cys 1520 1525 1530 His Glu Thr Gly Arg Leu Glu Pro Ser Trp Ala Asp Val Arg Asn 1535 1540 1545 Asp Met Ile Ser Tyr Gly Gly Gly Trp Arg Leu Gly Asp Lys Trp 1550 1555 1560 Asp Lys Glu Glu Asp Val Gln Val Leu Ala Ile Glu Pro Gly Lys 1565 1570 1575 Asn Pro Lys His Val Gln Thr Lys Pro Gly Leu Phe Lys Thr Leu 1580 1585 1590 Thr Gly Glu Ile Gly Ala Val Thr Leu Asp Phe Lys Pro Gly Thr 1595 1600 1605 Ser Gly Ser Pro Ile Ile Asn Arg Lys Gly Lys Val Ile Gly Leu 1610 1615 1620 Tyr Gly Asn Gly Val Val Thr Lys Ser Gly Asp Tyr Val Ser Ala 1625 1630 1635 Ile Thr Gln Ala Glu Arg Ile Gly Glu Pro Asp Tyr Glu Val Asp 1640 1645 1650 Glu Asp Ile Phe Arg Lys Lys Arg Leu Thr Ile Met Asp Leu His 1655 1660 1665 Pro Gly Ala Gly Lys Thr Lys Arg Ile Leu Pro Ser Ile Val Arg 1670 1675 1680 Glu Ala Leu Lys Arg Arg Leu Arg Thr Leu Ile Leu Ala Pro Thr 1685 1690 1695 Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu Pro 1700 1705 1710 Ile Arg Tyr Gln Thr Pro Ala Val Lys Ser Glu His Thr Gly Arg 1715 1720 1725 Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Thr Arg Leu 1730 1735 1740 Leu Ser Ser Thr Arg Val Pro Asn Tyr Asn Leu Ile Val Met Asp 1745 1750 1755 Glu Ala His Phe Thr Asp Pro Ser Ser Val Ala Ala Arg Gly Tyr 1760 1765 1770 Ile Ser Thr Arg Val Glu Met Gly Glu Ala Ala Ala Ile Phe Met 1775 1780 1785 Thr Ala Thr Pro Pro Gly Ala Thr Asp Pro Phe Pro Gln Ser Asn 1790 1795 1800 Ser Pro Ile Glu Asp Ile Glu Arg Glu Ile Pro Glu Arg Ser Trp 1805 1810 1815 Asn Thr Gly Phe Asp Trp Ile Thr Asp Tyr Gln Gly Lys Thr Val 1820 1825 1830 Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Asn Cys 1835 1840 1845 Leu Arg Lys Ser Gly Lys Lys Val Ile Gln Leu Ser Arg Lys Thr 1850 1855 1860 Phe Asp Thr Glu Tyr Pro Lys Thr Lys Leu Thr Asp Trp Asp Phe 1865 1870 1875 Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Arg Ala 1880 1885 1890 Gly Arg Val Ile Asp Pro Arg Arg Cys Leu Lys Pro Val Ile Leu 1895 1900 1905 Thr Asp Gly Pro Glu Arg Val Ile Leu Ala Gly Pro Ile Pro Val 1910 1915 1920 Thr Pro Ala Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg Asn 1925 1930 1935 Pro Ala Gln Glu Asp Asp Gln Tyr Val Phe Ser Gly Asp Pro Leu 1940 1945 1950 Lys Asn Asp Glu Asp His Ala His Trp Thr Glu Ala Lys Met Leu 1955 1960 1965 Leu Asp Asn Ile Tyr Thr Pro Glu Gly Ile Ile Pro Thr Leu Phe 1970 1975 1980 Gly Pro Glu Arg Glu Lys Thr Gln Ala Ile Asp Gly Glu Phe Arg 1985 1990 1995 Leu Arg Gly Glu Gln Arg Lys Thr Phe Val Glu Leu Met Arg Arg 2000 2005 2010 Gly Asp Leu Pro Val Trp Leu Ser Tyr Lys Val Ala Ser Ala Gly 2015 2020 2025 Ile Ser Tyr Lys Asp Arg Glu Trp Cys Phe Thr Gly Glu Arg Asn 2030 2035 2040 Asn Gln Ile Leu Glu Glu Asn Met Glu Val Glu Ile Trp Thr Arg 2045 2050 2055 Glu Gly Glu Lys Lys Lys Leu Arg Pro Arg Trp Leu Asp Ala Arg 2060 2065 2070 Val Tyr Ala Asp Pro Met Ala Leu Lys Asp Phe Lys Glu Phe Ala 2075 2080 2085 Ser Gly Arg Lys Ser Ile Thr Leu Asp Ile Leu Thr Glu Ile Ala 2090 2095 2100 Ser Leu Pro Thr Tyr Leu Ser Ser Arg Ala Lys Leu Ala Leu Asp 2105 2110 2115 Asn Ile Val Met Leu His Thr Thr Glu Arg Gly Gly Arg Ala Tyr 2120 2125 2130 Gln His Ala Leu Asn Glu Leu Pro Glu Ser Leu Glu Thr Leu Met 2135 2140 2145 Leu Val Ala Leu Leu Gly Ala Met Thr Ala Gly Ile Phe Leu Phe 2150 2155 2160 Phe Met Gln Gly Lys Gly Ile Gly Lys Leu Ser Met Gly Leu Ile 2165 2170 2175 Thr Ile Ala Val Ala Ser Gly Leu Leu Trp Val Ala Glu Ile Gln 2180 2185 2190 Pro Gln Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe Leu Met 2195 2200 2205 Val Leu Leu Ile Pro Glu Pro Glu Xaa Gln Arg Thr Pro Gln Asp 2210 2215 2220 Asn Gln Leu Ile Tyr Val Ile Leu Thr Ile Leu Thr Ile Ile Gly 2225 2230 2235 Leu Ile Ala Ala Asn Glu Met Gly Leu Ile Glu Lys Thr Lys Thr 2240 2245 2250 Asp Xaa Gly Phe Tyr Gln Val Lys Thr Glu Thr Thr Ile Leu Asp 2255 2260 2265 Val Asp Leu Arg Pro Ala Ser Ala Trp Thr Leu Tyr Ala Val Ala 2270 2275 2280 Thr Thr Ile Leu Thr Pro Met Leu Arg His Thr Ile Glu Asn Thr 2285 2290 2295 Ser Ala Asn Leu Ser Leu Ala Ala Ile Ala Asn Gln Ala Ala Val 2300 2305 2310 Leu Met Gly Leu Gly Lys Gly Trp Pro Leu His Arg Met Asp Leu 2315 2320 2325 Gly Val Pro Leu Leu Ala Met Gly Cys Tyr Ser Gln Val Asn Pro 2330 2335 2340 Xaa Thr Leu Thr Ala Ser Leu Val Met Leu Xaa Val His Tyr Ala 2345 2350 2355 Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr Arg Glu Ala Gln 2360 2365 2370 Lys Arg Thr Ala Ala Gly Ile Met Lys Asn Pro Thr Val Asp Gly 2375 2380 2385 Ile Thr Val Ile Asp Leu Glu Pro Ile Ser Tyr Asp Pro Lys Phe 2390 2395 2400 Glu Lys Gln Leu Gly Gln Val Met Leu Leu Val Leu Cys Ala Gly 2405 2410 2415 Gln Leu Leu Leu Met Arg Thr Thr Trp Ala Phe Cys Glu Val Leu 2420 2425 2430 Thr Leu Ala Thr Gly Pro Ile Leu Thr Leu Trp Glu Gly Asn Pro 2435 2440 2445 Gly Arg Phe Trp Asn Thr Thr Ile Ala Val Ser Thr Ala Asn Ile 2450 2455 2460 Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu Ala Phe Ser Leu 2465 2470 2475 Ile Lys Asn Val Gln Thr Pro Arg Arg Gly Thr Gly Thr Thr Gly 2480 2485 2490 Glu Thr Leu Gly Glu Lys Trp Lys Arg Gln Leu Asn Ser Leu Asp 2495 2500 2505 Arg Lys Glu Phe Glu Glu Tyr Lys Arg Ser Gly Ile Leu Glu Val 2510 2515 2520 Asp Arg Thr Glu Ala Lys Ser Ala Leu Lys Asp Gly Ser Lys Ile 2525 2530 2535 Lys His Ala Val Ser Arg Gly Ser Ser Lys Ile Arg Trp Ile Val 2540 2545 2550 Glu Arg Gly Met Val Lys Pro Lys Gly Lys Val Val Asp Leu Gly 2555 2560 2565 Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Met Ala Thr Leu Lys Asn 2570 2575 2580 Val Thr Glu Val Lys Gly Tyr Thr Lys Gly Gly Pro Gly His Glu 2585 2590 2595 Glu Pro Ile Pro Met Ala Thr Tyr Gly Trp Asn Leu Val Lys Leu 2600 2605 2610 His Ser Gly Val Asp Val Phe Tyr Lys Pro Thr Glu Gln Val Asp 2615 2620 2625 Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Ser Asn Pro Thr Ile 2630 2635 2640 Glu Glu Gly Arg Thr Leu Arg Val Leu Lys Met Val Glu Pro Trp 2645 2650 2655 Leu Ser Ser Lys Pro Glu Phe Cys Ile Lys Val Leu Asn Pro Tyr 2660 2665 2670 Met Pro Thr Val Ile Glu Glu Leu Glu Lys Leu Gln Arg Lys His 2675 2680 2685 Gly Gly Asn Leu Val Arg Cys Pro Leu Ser Arg Asn Ser Thr His 2690 2695 2700 Glu Met Tyr Trp Val Ser Gly Ala Ser Gly Asn Ile Val Ser Ser 2705 2710 2715 Val Asn Thr Thr Ser Lys Met Leu Leu Asn Arg Phe Thr Thr Arg 2720 2725 2730 His Arg Lys Pro Thr Tyr Glu Lys Asp Val Asp Leu Gly Ala Gly 2735 2740 2745 Thr Arg Ser Val Ser Thr Glu Thr Glu Lys Pro Asp Met Thr Ile 2750 2755 2760 Ile Gly Arg Arg Leu Gln Arg Leu Gln Glu Glu His Lys Glu Thr 2765 2770 2775 Trp His Tyr Asp Gln Glu Asn Pro Tyr Arg Thr Trp Ala Tyr His 2780 2785 2790 Gly Ser Tyr Glu Ala Pro Ser Thr Gly Ser Ala Ser Ser Met Val 2795 2800 2805 Asn Gly Val Val Lys Leu Leu Thr Lys Pro Trp Asp Val Val Pro 2810 2815 2820 Met Val Thr Gln Leu Ala Met Thr Asp Thr Thr Pro Phe Gly Gln 2825 2830 2835 Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg Thr Pro Gln Pro 2840 2845 2850 Lys Pro Gly Thr Arg Met Val Met Thr Thr Thr Ala Asn Trp Leu 2855 2860 2865 Trp Ala Leu Leu Gly Lys Lys Lys Xaa Pro Arg Leu Cys Thr Arg 2870 2875 2880 Glu Glu Phe Ile Ser Lys Val Arg Ser Asn Ala Ala Ile Gly Ala 2885 2890 2895 Val Phe Gln Glu Glu Gln Gly Trp Thr Ser Ala Ser Glu Ala Val 2900 2905 2910 Asn Asp Ser Arg Phe Trp Glu Leu Val Asp Lys Glu Arg Ala Leu 2915 2920 2925 His Gln Glu Gly Lys Cys Glu Ser Cys Val Tyr Asn Met Met Gly 2930 2935 2940 Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Arg Ala Lys Gly Ser 2945 2950 2955 Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg Phe Leu Glu Phe 2960 2965 2970 Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp Phe Gly Arg Glu 2975 2980 2985 Asn Ser Trp Ser Gly Val Glu Gly Glu Gly Leu His Arg Leu Gly 2990 2995 3000 Tyr Ile Leu Glu Glu Ile Asp Lys Lys Asp Gly Asp Leu Met Tyr 3005 3010 3015 Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile Thr Glu Asp Asp 3020 3025 3030 Leu Gln Asn Glu Glu Leu Ile Thr Glu Gln Met Ala Pro His His 3035 3040 3045 Lys Ile Leu Ala Lys Ala Ile Phe Lys Leu Thr Tyr Gln Asn Lys 3050 3055 3060 Val Val Lys Val Leu Arg Pro Thr Pro Arg Gly Ala Val Met Asp 3065 3070 3075 Ile Ile Ser Arg Lys Asp Gln Arg Gly Ser Gly Gln Val Gly Thr 3080 3085 3090 Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Val Gln Leu Ile Arg 3095 3100 3105 Gln Met Glu Ala Glu Gly Val Ile Thr Gln Asp Asp Met Gln Asn 3110 3115 3120 Pro Lys Gly Leu Lys Glu Arg Val Glu Lys Trp Leu Lys Glu Cys 3125 3130 3135 Gly Val Asp Arg Leu Lys Arg Met Ala Ile Ser Gly Asp Asp Cys 3140 3145 3150 Val Val Lys Pro Leu Asp Glu Arg Phe Gly Thr Ser Leu Leu Phe 3155 3160 3165 Leu Asn Asp Met Gly Lys Val Arg Lys Asp Ile Pro Gln Trp Glu 3170 3175 3180 Pro Ser Lys Gly Trp Lys Asn Trp Gln Glu Val Pro Phe Cys Ser 3185 3190 3195 His His Phe His Lys Ile Phe Met Lys Asp Gly Arg Ser Leu Val 3200 3205 3210 Val Pro Cys Arg Asn Gln Asp Glu Leu Ile Gly Arg Ala Arg Ile 3215 3220 3225 Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala Cys Leu Gly 3230 3235 3240 Lys Ala Tyr Ala Gln Met Trp Ser Leu Met Tyr Phe His Arg Arg 3245 3250 3255 Asp Leu Arg Leu Ala Ser Met Ala Ile Cys Ser Ala Val Pro Thr 3260 3265 3270 Glu Trp Phe Pro Thr Ser Arg Thr Thr Trp Ser Ile His Ala His 3275 3280 3285 His Gln Trp Met Thr Thr Glu Asp Met Leu Lys Val Trp Asn Arg 3290 3295 3300 Val Trp Ile Glu Asp Asn Pro Asn Met Thr Asp Lys Thr Pro Val 3305 3310 3315 His Ser Trp Glu Asp Ile Pro Tyr Leu Gly Lys Arg Glu Asp Leu 3320 3325 3330 Trp Cys Gly Ser Leu Ile Gly Leu Ser Ser Arg Ala Thr Trp Ala 3335 3340 3345 Lys Asn Ile His Thr Ala Ile Thr Gln Val Arg Asn Leu Ile Gly 3350 3355 3360 Lys Glu Glu Tyr Val Asp Tyr Met Pro Val Met Lys Arg Tyr Ser 3365 3370 3375 Ala Pro Ser Glu Ser Glu Gly Val Leu 3380 3385 <210> 5 <211> 3392 <212> PRT <213> Dengue virus type 1 <220> <221> VARIANT <222> (899)..(899) <223> Xaa stands for Met or Ile <220> <221> VARIANT <222> (2353)..(2353) <223> Xaa stands for Thr or Ile <400> 5 Met Asn Asn Gln Arg Lys Lys Thr Ala Arg Pro Ser Phe Asn Met Leu 1 5 10 15 Lys Arg Ala Arg Asn Arg Val Ser Thr Val Ser Gln Leu Ala Lys Arg 20 25 30 Phe Ser Lys Gly Leu Leu Ser Gly Gln Gly Pro Met Lys Leu Val Met 35 40 45 Ala Phe Ile Ala Phe Leu Arg Phe Leu Ala Ile Pro Pro Thr Ala Gly 50 55 60 Ile Leu Ala Arg Trp Gly Ser Phe Lys Lys Asn Gly Ala Ile Lys Val 65 70 75 80 Leu Arg Gly Phe Lys Lys Glu Ile Ser Asn Met Leu Asn Ile Met Asn 85 90 95 Arg Arg Lys Arg Ser Val Thr Met Leu Leu Met Leu Met Pro Thr Ala 100 105 110 Leu Ala Phe His Leu Thr Thr Arg Gly Gly Glu Pro His Met Ile Val 115 120 125 Ser Lys Gln Glu Arg Gly Lys Ser Leu Leu Phe Lys Thr Ser Ala Gly 130 135 140 Val Asn Met Cys Thr Leu Ile Ala Met Asp Leu Gly Glu Leu Cys Glu 145 150 155 160 Asp Thr Met Thr Tyr Lys Cys Pro Arg Ile Thr Glu Ala Glu Pro Asp 165 170 175 Asp Val Asp Cys Trp Cys Asn Ala Thr Asp Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Ser Gln Thr Gly Glu His Arg Arg Asp Lys Arg Ser Val Ala 195 200 205 Leu Ala Pro His Val Gly Leu Gly Leu Glu Thr Arg Thr Glu Thr Trp 210 215 220 Met Ser Ser Glu Gly Ala Trp Lys Gln Ile Gln Arg Val Glu Thr Trp 225 230 235 240 Ala Leu Arg His Pro Gly Phe Thr Val Ile Ala Leu Phe Leu Ala His 245 250 255 Ala Ile Gly Thr Ser Ile Thr Gln Lys Gly Ile Ile Phe Ile Leu Leu 260 265 270 Met Leu Val Thr Pro Ser Met Ala Met Arg Cys Val Gly Ile Gly Ser 275 280 285 Arg Asp Phe Val Glu Gly Leu Ser Gly Ala Thr Trp Val Asp Val Val 290 295 300 Leu Glu His Gly Ser Cys Val Thr Thr Met Ala Lys Asp Lys Pro Thr 305 310 315 320 Leu Asp Ile Glu Leu Leu Lys Thr Glu Val Thr Asn Pro Ala Val Leu 325 330 335 Arg Lys Leu Cys Ile Glu Ala Lys Ile Ser Asn Thr Thr Thr Asp Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Ala Thr Leu Val Glu Glu Gln Asp Ala 355 360 365 Asn Phe Val Cys Arg Arg Thr Phe Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Ser Leu Leu Thr Cys Ala Lys Phe Lys 385 390 395 400 Cys Val Thr Lys Leu Glu Gly Lys Ile Val Gln Tyr Glu Asn Leu Lys 405 410 415 Tyr Ser Val Ile Val Thr Val His Thr Gly Asp Gln His Gln Val Gly 420 425 430 Asn Glu Ser Thr Glu His Gly Thr Thr Ala Thr Ile Thr Pro Gln Ala 435 440 445 Pro Thr Thr Glu Ile Gln Leu Thr Asp Tyr Gly Ala Leu Thr Leu Asp 450 455 460 Cys Ser Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Val Leu Leu Thr 465 470 475 480 Met Lys Lys Lys Ser Trp Leu Val His Lys Gln Trp Phe Leu Asp Leu 485 490 495 Pro Leu Pro Trp Thr Ser Gly Ala Ser Thr Ser Gln Glu Thr Trp Asn 500 505 510 Arg Gln Asp Leu Leu Val Thr Phe Lys Thr Ala His Ala Lys Lys Gln 515 520 525 Glu Val Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu 530 535 540 Thr Gly Ala Thr Glu Ile Gln Thr Ser Gly Thr Thr Thr Ile Phe Ala 545 550 555 560 Gly His Leu Lys Cys Arg Leu Arg Met Asp Lys Leu Thr Leu Lys Gly 565 570 575 Met Ser Tyr Val Met Cys Thr Gly Ser Phe Lys Leu Glu Lys Glu Val 580 585 590 Ala Glu Thr Gln His Gly Thr Val Leu Val Gln Ile Lys Tyr Glu Gly 595 600 605 Thr Asp Ala Pro Cys Lys Ile Pro Phe Ser Thr Gln Asp Glu Lys Gly 610 615 620 Val Thr Gln Asn Gly Arg Leu Ile Thr Ala Asn Pro Ile Val Thr Asp 625 630 635 640 Lys Glu Lys Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser 645 650 655 Tyr Ile Val Ile Gly Ala Gly Glu Lys Ala Leu Lys Leu Ser Trp Phe 660 665 670 Lys Lys Gly Ser Ser Ile Gly Lys Met Phe Glu Ala Thr Ala Arg Gly 675 680 685 Ala Arg Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 690 695 700 Ile Gly Gly Val Phe Thr Ser Val Gly Lys Leu Val His Gln Ile Phe 705 710 715 720 Gly Thr Ala Tyr Gly Val Leu Phe Ser Gly Val Ser Trp Thr Met Lys 725 730 735 Ile Gly Ile Gly Val Leu Leu Thr Trp Leu Gly Leu Asn Ser Arg Asn 740 745 750 Thr Ser Leu Ser Met Thr Cys Ile Ala Val Gly Leu Val Thr Leu Tyr 755 760 765 Leu Gly Val Met Val Gln Ala Asp Ser Gly Cys Val Ile Asn Trp Lys 770 775 780 Gly Arg Glu Leu Lys Cys Gly Ser Gly Ile Phe Val Thr Asn Glu Val 785 790 795 800 His Thr Trp Thr Glu Gln Tyr Lys Phe Gln Ala Asp Ser Pro Lys Arg 805 810 815 Leu Ser Ala Ala Ile Gly Lys Ala Trp Glu Glu Gly Val Cys Gly Ile 820 825 830 Arg Ser Ala Thr Arg Leu Glu Asn Ile Met Trp Lys Gln Ile Ser Asn 835 840 845 Glu Leu Asn His Ile Leu Leu Glu Asn Asp Met Lys Phe Thr Val Val 850 855 860 Val Gly Asp Val Ala Gly Ile Leu Ala Gln Gly Lys Lys Met Ile Arg 865 870 875 880 Pro Gln Pro Met Glu His Lys Tyr Ser Trp Lys Ser Trp Gly Lys Ala 885 890 895 Lys Ile Xaa Gly Ala Asp Val Gln Asn Ser Thr Phe Ile Ile Asp Gly 900 905 910 Pro Asn Thr Pro Glu Cys Pro Asp Asp Gln Arg Ala Trp Asn Ile Trp 915 920 925 Glu Val Glu Asp Tyr Gly Phe Gly Ile Phe Thr Thr Asn Ile Trp Leu 930 935 940 Lys Leu Arg Asp Ser Tyr Thr Gln Leu Cys Asp His Arg Leu Met Ser 945 950 955 960 Ala Ala Ile Lys Asp Ser Lys Ala Val His Ala Asp Met Gly Tyr Trp 965 970 975 Ile Glu Ser Glu Lys Asn Glu Thr Trp Lys Leu Ala Arg Ala Ser Phe 980 985 990 Ile Glu Val Lys Thr Cys Ile Trp Pro Lys Ser His Thr Leu Trp Ser 995 1000 1005 Asn Gly Val Leu Glu Ser Glu Met Ile Ile Pro Lys Ile Tyr Gly 1010 1015 1020 Gly Pro Ile Ser Gln His Asn Tyr Arg Pro Gly Tyr Phe Thr Gln 1025 1030 1035 Thr Val Gly Pro Trp His Leu Gly Lys Leu Glu Leu Asp Phe Asp 1040 1045 1050 Leu Cys Glu Gly Thr Thr Val Val Val Asp Glu His Cys Gly Asn 1055 1060 1065 Arg Gly Pro Ser Leu Arg Thr Thr Thr Val Thr Gly Lys Ile Ile 1070 1075 1080 His Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Phe 1085 1090 1095 Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Val 1100 1105 1110 Lys Glu Lys Glu Glu Asn Leu Val Lys Ser Met Val Ser Ala Gly 1115 1120 1125 Ser Gly Glu Val Asp Ser Phe Ser Leu Gly Leu Leu Cys Ile Ser 1130 1135 1140 Ile Met Ile Glu Glu Val Met Arg Ser Arg Trp Ser Arg Lys Met 1145 1150 1155 Leu Met Thr Gly Thr Leu Ala Val Phe Leu Leu Leu Ile Met Gly 1160 1165 1170 Gln Leu Thr Trp Asn Asp Leu Ile Arg Leu Cys Ile Met Val Gly 1175 1180 1185 Ala Asn Ala Ser Asp Arg Met Gly Met Gly Thr Thr Tyr Leu Ala 1190 1195 1200 Leu Met Ala Thr Phe Lys Met Arg Pro Met Phe Ala Val Gly Leu 1205 1210 1215 Leu Phe Arg Arg Leu Thr Ser Arg Glu Val Leu Leu Leu Thr Ile 1220 1225 1230 Gly Leu Ser Leu Val Ala Ser Val Glu Leu Pro Asn Ser Leu Glu 1235 1240 1245 Glu Leu Gly Asp Gly Leu Ala Met Gly Ile Met Ile Leu Lys Leu 1250 1255 1260 Leu Thr Asp Phe Gln Ser His Gln Leu Trp Ala Thr Leu Leu Ser 1265 1270 1275 Leu Thr Phe Ile Lys Thr Thr Phe Ser Leu His Tyr Ala Trp Lys 1280 1285 1290 Thr Met Ala Met Val Leu Ser Ile Val Ser Leu Phe Pro Leu Cys 1295 1300 1305 Leu Ser Thr Thr Ser Gln Lys Thr Thr Trp Leu Pro Val Leu Leu 1310 1315 1320 Gly Ser Leu Gly Cys Lys Pro Leu Thr Met Phe Leu Ile Ala Glu 1325 1330 1335 Asn Lys Ile Trp Gly Arg Lys Ser Trp Pro Leu Asn Glu Gly Ile 1340 1345 1350 Met Ala Val Gly Ile Val Ser Ile Leu Leu Ser Ser Leu Leu Lys 1355 1360 1365 Asn Asp Val Pro Leu Ala Gly Pro Leu Ile Ala Gly Gly Met Leu 1370 1375 1380 Ile Ala Cys Tyr Val Ile Ser Gly Ser Ser Ala Asp Leu Ser Leu 1385 1390 1395 Glu Lys Ala Ala Glu Val Ser Trp Glu Glu Glu Ala Glu His Ser 1400 1405 1410 Gly Ala Ser His Asn Ile Leu Val Glu Val Gln Asp Asp Gly Thr 1415 1420 1425 Met Lys Ile Lys Asp Glu Glu Arg Asp Asp Thr Leu Thr Ile Leu 1430 1435 1440 Leu Lys Ala Thr Leu Leu Ala Val Ser Gly Val Tyr Pro Leu Ser 1445 1450 1455 Ile Pro Ala Thr Leu Phe Val Trp Tyr Phe Trp Gln Lys Lys Lys 1460 1465 1470 Gln Arg Ser Gly Val Leu Trp Asp Thr Pro Ser Pro Pro Glu Val 1475 1480 1485 Glu Arg Ala Val Leu Asp Asp Gly Ile Tyr Arg Ile Met Gln Arg 1490 1495 1500 Gly Leu Leu Gly Arg Ser Gln Val Gly Val Gly Val Phe Gln Glu 1505 1510 1515 Asn Val Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu 1520 1525 1530 Met Tyr Gln Gly Lys Arg Leu Glu Pro Ser Trp Ala Ser Val Lys 1535 1540 1545 Lys Asp Leu Ile Ser Tyr Gly Gly Gly Trp Arg Leu Gln Gly Ser 1550 1555 1560 Trp Asn Thr Gly Glu Glu Val Gln Val Ile Ala Val Glu Pro Gly 1565 1570 1575 Lys Asn Pro Lys Asn Val Gln Thr Ala Pro Gly Thr Phe Lys Thr 1580 1585 1590 Pro Glu Gly Glu Val Gly Ala Ile Ala Leu Asp Phe Lys Pro Gly 1595 1600 1605 Thr Ser Gly Ser Pro Ile Val Asn Arg Glu Gly Lys Ile Val Gly 1610 1615 1620 Leu Tyr Gly Asn Gly Val Val Thr Thr Ser Gly Thr Tyr Val Ser 1625 1630 1635 Ala Ile Ala Gln Ala Lys Ala Ser Gln Glu Gly Pro Leu Pro Glu 1640 1645 1650 Ile Glu Asp Glu Val Phe Arg Lys Arg Lys Leu Thr Ile Met Asp 1655 1660 1665 Leu His Pro Gly Ser Gly Lys Thr Arg Arg Tyr Leu Pro Ala Ile 1670 1675 1680 Val Arg Glu Ala Ile Lys Arg Lys Leu Arg Thr Leu Ile Leu Ala 1685 1690 1695 Pro Thr Arg Val Val Ala Ser Glu Met Ala Glu Ala Leu Lys Gly 1700 1705 1710 Met Pro Ile Arg Tyr Gln Thr Thr Ala Val Lys Ser Glu His Thr 1715 1720 1725 Gly Lys Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met 1730 1735 1740 Arg Leu Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Met Ile Ile 1745 1750 1755 Met Asp Glu Ala His Phe Thr Asp Pro Ser Ser Ile Ala Ala Arg 1760 1765 1770 Gly Tyr Ile Ser Thr Arg Val Gly Met Gly Glu Ala Ala Ala Ile 1775 1780 1785 Phe Met Thr Ala Thr Pro Pro Gly Ser Val Glu Ala Phe Pro Gln 1790 1795 1800 Ser Asn Ala Val Ile Gln Asp Glu Glu Arg Asp Ile Pro Glu Arg 1805 1810 1815 Ser Trp Asn Ser Gly Tyr Glu Trp Ile Thr Asp Phe Pro Gly Lys 1820 1825 1830 Thr Val Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala 1835 1840 1845 Asn Cys Leu Arg Lys Asn Gly Lys Arg Val Ile Gln Leu Ser Arg 1850 1855 1860 Lys Thr Phe Asp Thr Glu Tyr Gln Lys Thr Lys Asn Asn Asp Trp 1865 1870 1875 Asp Tyr Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe 1880 1885 1890 Arg Ala Asp Arg Val Ile Asp Pro Arg Arg Cys Leu Lys Pro Val 1895 1900 1905 Ile Leu Lys Asp Gly Pro Glu Arg Val Ile Leu Ala Gly Pro Met 1910 1915 1920 Pro Val Thr Val Ala Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly 1925 1930 1935 Arg Asn His Asn Lys Glu Gly Asp Gln Tyr Ile Tyr Met Gly Gln 1940 1945 1950 Pro Leu Asn Asn Asp Glu Asp His Ala His Trp Thr Glu Ala Lys 1955 1960 1965 Met Leu Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ala 1970 1975 1980 Leu Phe Glu Pro Glu Arg Glu Lys Ser Ala Ala Ile Asp Gly Glu 1985 1990 1995 Tyr Arg Leu Arg Gly Glu Ala Arg Lys Thr Phe Val Glu Leu Met 2000 2005 2010 Arg Arg Gly Asp Leu Pro Val Trp Leu Ser Tyr Lys Val Ala Ser 2015 2020 2025 Glu Gly Phe Gln Tyr Ser Asp Arg Arg Trp Cys Phe Asp Gly Glu 2030 2035 2040 Arg Asn Asn Gln Val Leu Glu Glu Asn Met Asp Val Glu Ile Trp 2045 2050 2055 Thr Lys Glu Gly Glu Arg Lys Lys Leu Arg Pro Arg Trp Leu Asp 2060 2065 2070 Ala Arg Thr Tyr Ser Asp Pro Leu Ala Leu Arg Glu Phe Lys Glu 2075 2080 2085 Phe Ala Ala Gly Arg Arg Ser Val Ser Gly Asp Leu Ile Leu Glu 2090 2095 2100 Ile Gly Lys Leu Pro Gln His Leu Thr Gln Arg Ala Gln Asn Ala 2105 2110 2115 Leu Asp Asn Leu Val Met Leu His Asn Ser Glu Gln Gly Gly Arg 2120 2125 2130 Ala Tyr Arg His Ala Met Glu Glu Leu Pro Asp Thr Ile Glu Thr 2135 2140 2145 Leu Met Leu Leu Ala Leu Ile Ala Val Leu Thr Gly Gly Val Thr 2150 2155 2160 Leu Phe Phe Leu Ser Gly Lys Gly Leu Gly Lys Thr Ser Ile Gly 2165 2170 2175 Leu Leu Cys Val Met Ala Ser Ser Val Leu Leu Trp Met Ala Ser 2180 2185 2190 Val Glu Pro His Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe 2195 2200 2205 Leu Met Val Leu Leu Ile Pro Glu Pro Asp Arg Gln Arg Thr Pro 2210 2215 2220 Gln Asp Asn Gln Leu Ala Tyr Val Val Ile Gly Leu Leu Phe Met 2225 2230 2235 Ile Leu Thr Val Ala Ala Asn Glu Met Gly Leu Leu Glu Thr Thr 2240 2245 2250 Lys Lys Asp Leu Gly Ile Gly His Val Ala Val Glu Asn His His 2255 2260 2265 His Ala Ala Met Leu Asp Val Asp Leu His Pro Ala Ser Ala Trp 2270 2275 2280 Thr Leu Tyr Ala Val Ala Thr Thr Ile Ile Thr Pro Met Met Arg 2285 2290 2295 His Thr Ile Glu Asn Thr Thr Ala Asn Ile Ser Leu Thr Ala Ile 2300 2305 2310 Ala Asn Gln Ala Ala Ile Leu Met Gly Leu Asp Lys Gly Trp Pro 2315 2320 2325 Ile Ser Lys Met Asp Ile Gly Val Pro Leu Leu Ala Leu Gly Cys 2330 2335 2340 Tyr Ser Gln Val Asn Pro Leu Thr Leu Xaa Ala Ala Val Leu Met 2345 2350 2355 Leu Val Ala His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys 2360 2365 2370 Ala Thr Arg Glu Ala Gln Lys Arg Thr Ala Ala Gly Ile Met Lys 2375 2380 2385 Asn Pro Thr Ile Asp Gly Ile Val Ala Ile Asp Leu Asp Pro Val 2390 2395 2400 Val Tyr Asp Ala Lys Phe Glu Lys Gln Leu Gly Gln Ile Met Leu 2405 2410 2415 Leu Ile Leu Cys Thr Ser Gln Ile Leu Leu Met Arg Thr Thr Trp 2420 2425 2430 Ala Leu Cys Glu Ser Ile Thr Leu Ala Thr Gly Pro Leu Thr Thr 2435 2440 2445 Leu Trp Glu Gly Ser Pro Gly Lys Phe Trp Asn Thr Thr Ile Ala 2450 2455 2460 Val Ser Met Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala 2465 2470 2475 Gly Leu Ala Phe Ser Leu Met Lys Ser Leu Gly Gly Gly Arg Arg 2480 2485 2490 Gly Thr Gly Ala Gln Gly Glu Thr Leu Gly Glu Lys Trp Lys Arg 2495 2500 2505 Gln Leu Asn Gln Leu Ser Lys Ser Glu Phe Asn Thr Tyr Lys Arg 2510 2515 2520 Ser Gly Ile Met Glu Val Asp Arg Ser Glu Ala Lys Glu Gly Leu 2525 2530 2535 Lys Arg Gly Glu Thr Thr Lys His Ala Val Ser Arg Gly Thr Ala 2540 2545 2550 Lys Leu Arg Trp Phe Val Glu Arg Asn Leu Val Lys Pro Glu Gly 2555 2560 2565 Lys Val Ile Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr 2570 2575 2580 Cys Ala Gly Leu Lys Lys Val Thr Glu Val Lys Gly Tyr Thr Lys 2585 2590 2595 Gly Gly Pro Gly His Glu Glu Pro Ile Pro Met Ala Thr Tyr Gly 2600 2605 2610 Trp Asn Leu Val Lys Leu His Ser Gly Lys Asp Val Phe Phe Ile 2615 2620 2625 Pro Pro Glu Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser 2630 2635 2640 Ser Pro Asn Pro Thr Ile Glu Glu Gly Arg Thr Leu Arg Val Leu 2645 2650 2655 Lys Met Val Glu Pro Trp Leu Arg Gly Asn Gln Phe Cys Ile Lys 2660 2665 2670 Ile Leu Asn Pro Tyr Met Pro Ser Val Val Glu Thr Leu Glu Gln 2675 2680 2685 Met Gln Arg Lys His Gly Gly Met Leu Val Arg Asn Pro Leu Ser 2690 2695 2700 Arg Asn Ser Thr His Glu Met Tyr Trp Val Ser Cys Gly Thr Gly 2705 2710 2715 Asn Ile Val Ser Ala Val Asn Met Thr Ser Arg Met Leu Leu Asn 2720 2725 2730 Arg Phe Thr Met Ala His Arg Lys Pro Thr Tyr Glu Arg Asp Val 2735 2740 2745 Asp Leu Gly Ala Gly Thr Arg His Val Ala Val Glu Pro Glu Val 2750 2755 2760 Ala Asn Leu Asp Ile Ile Gly Gln Arg Ile Glu Asn Ile Lys His 2765 2770 2775 Glu His Lys Ser Thr Trp His Tyr Asp Glu Asp Asn Pro Tyr Lys 2780 2785 2790 Thr Trp Ala Tyr His Gly Ser Tyr Glu Val Lys Pro Ser Gly Ser 2795 2800 2805 Ala Ser Ser Met Val Asn Gly Val Val Lys Leu Leu Thr Lys Pro 2810 2815 2820 Trp Asp Val Ile Pro Met Val Thr Gln Ile Ala Met Thr Asp Thr 2825 2830 2835 Thr Pro Phe Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr 2840 2845 2850 Arg Thr Pro Lys Ala Lys Arg Gly Thr Ala Gln Ile Met Glu Val 2855 2860 2865 Thr Ala Arg Trp Leu Trp Gly Phe Leu Ser Arg Asn Lys Lys Pro 2870 2875 2880 Arg Ile Cys Thr Arg Glu Glu Phe Thr Arg Lys Val Arg Ser Asn 2885 2890 2895 Ala Ala Ile Gly Ala Val Phe Val Asp Glu Asn Gln Trp Asn Ser 2900 2905 2910 Ala Lys Glu Ala Val Glu Asp Asp Arg Phe Trp Asp Leu Val His 2915 2920 2925 Arg Glu Arg Glu Leu His Lys Gln Gly Lys Cys Ala Thr Cys Val 2930 2935 2940 Tyr Asn Met Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly 2945 2950 2955 Lys Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala 2960 2965 2970 Arg Phe Leu Glu Phe Glu Ala Leu Gly Phe Met Asn Glu Asp His 2975 2980 2985 Trp Phe Ser Arg Glu Asn Ser Leu Ser Gly Val Glu Gly Glu Gly 2990 2995 3000 Leu His Lys Leu Gly Tyr Ile Leu Arg Asp Ile Ser Lys Ile Pro 3005 3010 3015 Gly Gly Asn Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg 3020 3025 3030 Ile Thr Glu Asp Asp Leu Gln Asn Glu Ala Lys Ile Thr Asp Ile 3035 3040 3045 Met Glu Pro Glu His Ala Leu Leu Ala Lys Ser Ile Phe Lys Leu 3050 3055 3060 Thr Tyr Gln Asn Lys Val Val Arg Val Gln Arg Pro Ala Lys Asn 3065 3070 3075 Gly Thr Val Met Asp Val Ile Ser Arg Arg Asp Gln Arg Gly Ser 3080 3085 3090 Gly Gln Val Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu 3095 3100 3105 Ala Gln Leu Ile Arg Gln Met Glu Ser Glu Gly Ile Phe Ser Pro 3110 3115 3120 Asn Glu Leu Glu Thr Pro Asn Leu Ala Glu Arg Val Leu Asp Trp 3125 3130 3135 Leu Glu Lys Tyr Gly Val Glu Arg Leu Lys Arg Met Ala Ile Ser 3140 3145 3150 Gly Asp Asp Cys Val Val Lys Pro Ile Asp Asp Arg Phe Ala Thr 3155 3160 3165 Ala Leu Thr Ala Leu Asn Asp Met Gly Lys Val Arg Lys Asp Ile 3170 3175 3180 Pro Gln Trp Glu Pro Ser Lys Gly Trp Asn Asp Trp Gln Gln Val 3185 3190 3195 Pro Phe Cys Ser His His Phe His Gln Leu Ile Met Lys Asp Gly 3200 3205 3210 Arg Glu Ile Val Val Pro Cys Arg Asn Gln Asp Glu Leu Val Gly 3215 3220 3225 Arg Ala Arg Val Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr 3230 3235 3240 Ala Cys Leu Gly Lys Ser Tyr Ala Gln Met Trp Gln Leu Met Tyr 3245 3250 3255 Phe His Arg Arg Asp Leu Arg Leu Ala Ala Asn Ala Ile Cys Ser 3260 3265 3270 Ala Val Pro Val Asp Trp Val Pro Thr Ser Arg Thr Thr Trp Ser 3275 3280 3285 Ile His Ala His His Gln Trp Met Thr Thr Glu Asp Met Leu Ser 3290 3295 3300 Val Trp Asn Arg Val Trp Ile Glu Glu Asn Pro Trp Met Glu Asp 3305 3310 3315 Lys Thr Arg Ile Ser Ser Trp Glu Asp Val Pro Tyr Leu Gly Lys 3320 3325 3330 Arg Glu Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ala Arg 3335 3340 3345 Ala Thr Trp Ala Thr Asn Ile Gln Val Ala Ile Asn Gln Val Arg 3350 3355 3360 Arg Leu Ile Gly Asn Glu Asn Tyr Ser Asp Tyr Met Thr Ser Met 3365 3370 3375 Lys Arg Phe Lys Asn Glu Ser Glu Pro Glu Gly Ala Leu Trp 3380 3385 3390 <210> 6 <211> 3391 <212> PRT <213> Dengue virus type 2 <220> <221> VARIANT <222> (1371)..(1371) <223> Xaa stands for Val or Ile <400> 6 Met Asn Asn Gln Arg Lys Lys Ala Lys Asn Thr Pro Phe Asn Met Leu 1 5 10 15 Lys Arg Glu Arg Asn Arg Val Ser Thr Val Gln Gln Leu Thr Lys Arg 20 25 30 Phe Ser Leu Gly Met Leu Gln Gly Arg Gly Pro Leu Lys Leu Phe Met 35 40 45 Ala Leu Val Ala Phe Leu Arg Phe Leu Thr Ile Pro Pro Thr Ala Gly 50 55 60 Ile Leu Lys Arg Trp Gly Thr Ile Lys Lys Ser Lys Ala Ile Asn Val 65 70 75 80 Leu Arg Gly Phe Arg Lys Glu Ile Gly Arg Met Leu Asn Ile Leu Asn 85 90 95 Arg Arg Arg Arg Ser Ala Gly Met Ile Ile Met Leu Ile Pro Thr Val 100 105 110 Met Ala Phe His Leu Thr Thr Arg Asn Gly Glu Pro His Met Ile Val 115 120 125 Gly Ile Gln Glu Lys Gly Lys Ser Leu Leu Phe Lys Thr Glu Asn Gly 130 135 140 Val Asn Met Cys Thr Leu Met Ala Met Asp Leu Gly Glu Leu Cys Glu 145 150 155 160 Asp Thr Ile Thr Tyr Lys Cys Pro Leu Leu Arg Gln Asn Glu Pro Glu 165 170 175 Asp Ile Asp Cys Trp Cys Asn Ser Thr Ser Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Thr Thr Thr Gly Glu His Arg Arg Glu Lys Arg Ser Val Ala 195 200 205 Leu Val Pro His Val Gly Met Gly Leu Glu Thr Arg Thr Glu Thr Trp 210 215 220 Met Ser Ser Glu Gly Ala Trp Lys His Ala Gln Arg Ile Glu Thr Trp 225 230 235 240 Ile Leu Arg His Pro Gly Phe Thr Ile Met Ala Ala Ile Leu Ala Tyr 245 250 255 Thr Ile Gly Thr Thr His Phe Gln Arg Val Leu Ile Phe Ile Leu Leu 260 265 270 Thr Ala Val Ala Pro Ser Met Thr Met Arg Cys Ile Gly Ile Ser Asn 275 280 285 Arg Asp Phe Val Glu Gly Val Ser Gly Gly Ser Trp Val Asp Ile Val 290 295 300 Leu Glu His Gly Ser Cys Val Thr Thr Met Ala Lys Asn Lys Pro Thr 305 310 315 320 Leu Asp Phe Glu Leu Ile Lys Thr Glu Ala Lys Gln Pro Ala Thr Leu 325 330 335 Arg Lys Tyr Cys Ile Glu Ala Lys Leu Thr Asn Thr Thr Thr Glu Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Pro Ser Leu Lys Glu Glu Gln Asp Lys 355 360 365 Arg Phe Val Cys Lys His Ser Met Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Gly Ile Val Thr Cys Ala Met Phe Lys 385 390 395 400 Cys Lys Lys Asn Met Glu Gly Lys Ile Val Gln Pro Glu Asn Leu Glu 405 410 415 Tyr Thr Ile Val Val Thr Pro His Ser Gly Glu Glu His Ala Val Gly 420 425 430 Asn Asp Thr Gly Lys His Gly Lys Glu Ile Lys Val Thr Pro Gln Ser 435 440 445 Ser Ile Thr Glu Ala Glu Leu Thr Gly Tyr Gly Thr Val Thr Met Glu 450 455 460 Cys Ser Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Val Leu Leu Gln 465 470 475 480 Met Glu Asn Lys Ala Trp Leu Val His Arg Gln Trp Phe Leu Asp Leu 485 490 495 Pro Leu Pro Trp Leu Pro Gly Ala Asp Lys Gln Glu Ser Asn Trp Ile 500 505 510 Gln Lys Glu Thr Leu Val Thr Phe Lys Asn Pro His Ala Lys Lys Gln 515 520 525 Asp Val Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu 530 535 540 Thr Gly Ala Thr Glu Ile Gln Met Ser Ser Gly Asn Leu Leu Phe Thr 545 550 555 560 Gly His Leu Lys Cys Arg Leu Arg Met Asp Lys Leu Gln Leu Lys Gly 565 570 575 Met Ser Tyr Ser Met Cys Thr Gly Lys Phe Lys Val Val Lys Glu Ile 580 585 590 Ala Glu Thr Gln His Gly Thr Ile Val Ile Arg Val Gln Tyr Glu Gly 595 600 605 Asp Gly Ser Pro Cys Lys Ile Pro Phe Glu Ile Met Asp Leu Glu Lys 610 615 620 Arg Tyr Val Leu Gly Arg Leu Ile Thr Val Asn Pro Ile Val Thr Glu 625 630 635 640 Lys Asp Ser Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Asp Ser 645 650 655 Tyr Ile Ile Ile Gly Val Glu Pro Gly Gln Leu Lys Leu Asn Trp Phe 660 665 670 Lys Lys Gly Ser Ser Ile Gly Gln Met Phe Glu Thr Thr Met Arg Gly 675 680 685 Ala Lys Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 690 695 700 Leu Gly Gly Val Phe Thr Ser Ile Gly Lys Ala Leu His Gln Val Phe 705 710 715 720 Gly Ala Ile Tyr Gly Ala Ala Phe Ser Gly Val Ser Trp Thr Met Lys 725 730 735 Ile Leu Ile Gly Val Ile Ile Thr Trp Ile Gly Met Asn Ser Arg Ser 740 745 750 Thr Ser Leu Ser Val Ser Leu Val Leu Val Gly Ile Val Thr Leu Tyr 755 760 765 Leu Gly Val Met Val Gln Ala Asp Ser Gly Cys Val Val Ser Trp Lys 770 775 780 Asn Lys Glu Leu Lys Cys Gly Ser Gly Ile Phe Ile Thr Asp Asn Val 785 790 795 800 His Thr Trp Thr Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ser Lys 805 810 815 Leu Ala Ser Ala Ile Gln Lys Ala Gln Glu Glu Gly Ile Cys Gly Ile 820 825 830 Arg Ser Val Thr Arg Leu Glu Asn Leu Met Trp Lys Gln Ile Thr Pro 835 840 845 Glu Leu Asn His Ile Leu Ala Glu Asn Glu Val Lys Leu Thr Ile Met 850 855 860 Thr Gly Asp Ile Lys Gly Ile Met Gln Ala Gly Lys Arg Ser Leu Arg 865 870 875 880 Pro Gln Pro Thr Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala 885 890 895 Lys Met Leu Ser Thr Glu Ser Tyr Asn Gln Thr Phe Leu Ile Asp Gly 900 905 910 Pro Glu Thr Ala Glu Cys Pro Asn Thr Asn Arg Ala Trp Asn Ser Leu 915 920 925 Glu Val Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu 930 935 940 Lys Leu Lys Glu Lys Gln Asp Ala Phe Cys Asp Ser Lys Leu Met Ser 945 950 955 960 Ala Ala Ile Lys Asp Asn Arg Ala Val His Ala Asp Met Gly Tyr Trp 965 970 975 Ile Glu Ser Ala Leu Asn Asp Thr Trp Lys Ile Glu Lys Ala Ser Phe 980 985 990 Ile Glu Val Lys Asn Cys His Trp Pro Lys Ser His Thr Leu Trp Ser 995 1000 1005 Asn Gly Val Leu Glu Ser Glu Met Ile Ile Pro Lys Asn Leu Ala 1010 1015 1020 Gly Pro Val Ser Gln His Asn Tyr Arg Pro Gly Tyr His Thr Gln 1025 1030 1035 Val Ala Gly Pro Trp His Leu Gly Lys Leu Glu Met Asp Phe Asp 1040 1045 1050 Phe Cys Asp Gly Thr Thr Val Ile Val Thr Glu Asp Cys Gly Asn 1055 1060 1065 Arg Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys Leu Ile 1070 1075 1080 Thr Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Tyr 1085 1090 1095 Arg Gly Glu Asn Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu 1100 1105 1110 Lys Glu Lys Glu Glu Asn Leu Val Asn Ser Leu Val Thr Ala Gly 1115 1120 1125 His Gly Gln Val Asp Asn Phe Ser Leu Gly Val Leu Gly Met Ala 1130 1135 1140 Leu Phe Leu Glu Glu Met Leu Arg Thr Arg Val Gly Thr Lys His 1145 1150 1155 Ala Ile Leu Leu Val Ala Val Ser Phe Val Thr Leu Ile Thr Gly 1160 1165 1170 Asn Met Ser Phe Lys Asp Leu Gly Arg Val Val Val Met Val Gly 1175 1180 1185 Ala Thr Met Ala Asp Asp Ile Gly Met Gly Val Thr Tyr Leu Ala 1190 1195 1200 Leu Leu Ala Ala Phe Lys Val Arg Pro Thr Phe Ala Ala Gly Leu 1205 1210 1215 Leu Leu Arg Lys Leu Thr Ser Lys Glu Leu Met Met Thr Thr Ile 1220 1225 1230 Gly Ile Val Leu Leu Ser Gln Ser Thr Ile Pro Glu Thr Val Leu 1235 1240 1245 Glu Leu Thr Asp Ala Leu Ala Leu Gly Met Met Val Leu Lys Ile 1250 1255 1260 Val Arg Asn Met Glu Lys Tyr Gln Leu Ala Val Thr Ile Met Ala 1265 1270 1275 Ile Leu Cys Val Pro Asn Ala Val Ile Leu Gln Asn Ala Trp Lys 1280 1285 1290 Val Ser Cys Thr Ile Leu Ala Val Val Ser Val Ser Pro Leu Phe 1295 1300 1305 Leu Thr Ser Ser Gln Gln Lys Thr Asp Trp Ile Pro Leu Ala Leu 1310 1315 1320 Thr Ile Lys Gly Leu Asn Pro Thr Ala Ile Phe Leu Thr Thr Leu 1325 1330 1335 Ser Arg Thr Asn Lys Lys Arg Ser Trp Pro Leu Asn Glu Ala Ile 1340 1345 1350 Met Ala Val Gly Met Val Ser Ile Leu Ala Ser Ser Leu Leu Lys 1355 1360 1365 Asn Asp Xaa Pro Met Thr Gly Pro Leu Val Ala Gly Gly Leu Leu 1370 1375 1380 Thr Val Cys Tyr Val Leu Thr Gly Arg Ser Ala Asp Leu Glu Leu 1385 1390 1395 Glu Arg Ala Thr Asp Val Lys Trp Glu Asp Gln Ala Glu Ile Ser 1400 1405 1410 Gly Ser Ser Pro Ile Leu Ser Ile Thr Ile Ser Glu Asp Gly Ser 1415 1420 1425 Met Ser Ile Lys Asn Glu Glu Glu Glu Gln Thr Leu Thr Ile Leu 1430 1435 1440 Ile Arg Thr Gly Leu Leu Val Ile Ser Gly Leu Phe Pro Ile Ser 1445 1450 1455 Ile Pro Val Thr Ala Ala Ala Trp Tyr Leu Trp Glu Val Lys Lys 1460 1465 1470 Gln Arg Ala Gly Val Leu Trp Asp Val Pro Ser Pro Pro Pro Met 1475 1480 1485 Gly Lys Ala Glu Leu Glu Asp Gly Ala Tyr Arg Ile Lys Gln Lys 1490 1495 1500 Gly Ile Leu Gly Tyr Ser Gln Ile Gly Ala Gly Val Tyr Lys Glu 1505 1510 1515 Gly Thr Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu 1520 1525 1530 Met His Lys Gly Lys Arg Ile Glu Pro Ser Trp Ala Asp Val Lys 1535 1540 1545 Lys Asp Leu Ile Ser Tyr Gly Gly Gly Trp Lys Leu Glu Gly Glu 1550 1555 1560 Trp Lys Glu Gly Glu Glu Val Gln Val Leu Ala Leu Glu Pro Gly 1565 1570 1575 Lys Asn Pro Arg Ala Val Gln Thr Lys Pro Gly Leu Phe Lys Thr 1580 1585 1590 Asn Thr Gly Thr Ile Gly Ala Val Ser Leu Asp Phe Ser Pro Gly 1595 1600 1605 Thr Ser Gly Ser Pro Ile Ile Asp Lys Lys Gly Lys Val Val Gly 1610 1615 1620 Leu Tyr Gly Asn Gly Val Val Thr Arg Ser Gly Ala Tyr Val Ser 1625 1630 1635 Ala Ile Ala Gln Thr Glu Lys Ser Ile Glu Asp Asn Pro Glu Ile 1640 1645 1650 Lys Asp Asp Ile Phe Arg Lys Arg Arg Leu Thr Ile Met Asp Leu 1655 1660 1665 His Pro Gly Ala Gly Lys Thr Lys Arg Tyr Leu Pro Ala Ile Val 1670 1675 1680 Arg Glu Ala Ile Lys Arg Gly Leu Arg Thr Leu Ile Leu Ala Pro 1685 1690 1695 Thr Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu 1700 1705 1710 Pro Ile Arg Tyr Gln Thr Pro Ala Ile Arg Ala Glu His Thr Gly 1715 1720 1725 Arg Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met Arg 1730 1735 1740 Leu Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Leu Ile Ile Met 1745 1750 1755 Asp Glu Ala His Phe Thr Asp Pro Ala Ser Ile Ala Ala Arg Gly 1760 1765 1770 Tyr Ile Ser Thr Arg Val Glu Met Gly Glu Ala Ala Gly Ile Phe 1775 1780 1785 Met Thr Ala Thr Pro Pro Gly Ser Arg Asp Pro Phe Pro Gln Ser 1790 1795 1800 Asn Ala Pro Ile Ile Asp Glu Glu Arg Glu Ile Pro Glu Arg Ser 1805 1810 1815 Trp Asn Ser Gly His Glu Trp Val Thr Asp Phe Lys Gly Lys Thr 1820 1825 1830 Val Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Ala 1835 1840 1845 Cys Leu Arg Lys Asn Gly Lys Lys Val Ile Gln Leu Ser Arg Lys 1850 1855 1860 Thr Phe Asp Ser Glu Tyr Val Lys Thr Arg Thr Asn Asp Trp Asp 1865 1870 1875 Phe Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys 1880 1885 1890 Ala Glu Arg Val Ile Asp Pro Arg Arg Cys Met Lys Pro Val Ile 1895 1900 1905 Leu Thr Asp Gly Glu Glu Arg Val Ile Leu Ala Gly Pro Met Pro 1910 1915 1920 Val Thr His Ser Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg 1925 1930 1935 Asn Pro Lys Asn Glu Asn Asp Gln Tyr Ile Tyr Met Gly Glu Pro 1940 1945 1950 Leu Glu Asn Asp Glu Asp Cys Ala His Trp Lys Glu Ala Lys Met 1955 1960 1965 Leu Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ser Met 1970 1975 1980 Phe Glu Pro Glu Arg Glu Lys Val Asp Ala Ile Asp Gly Glu Tyr 1985 1990 1995 Arg Leu Arg Gly Glu Ala Arg Lys Thr Phe Val Asp Leu Met Arg 2000 2005 2010 Arg Gly Asp Leu Pro Val Trp Leu Ala Tyr Lys Val Ala Ala Glu 2015 2020 2025 Gly Ile Asn Tyr Ala Asp Arg Arg Trp Cys Phe Asp Gly Ile Lys 2030 2035 2040 Asn Asn Gln Ile Leu Glu Glu Asn Val Glu Val Glu Ile Trp Thr 2045 2050 2055 Lys Glu Gly Glu Arg Lys Lys Leu Lys Pro Arg Trp Leu Asp Ala 2060 2065 2070 Arg Ile Tyr Ser Asp Pro Leu Ala Leu Lys Glu Phe Lys Glu Phe 2075 2080 2085 Ala Ala Gly Arg Lys Ser Leu Thr Leu Asn Leu Ile Thr Glu Met 2090 2095 2100 Gly Arg Leu Pro Thr Phe Met Thr Gln Lys Thr Arg Asp Ala Leu 2105 2110 2115 Asp Asn Leu Ala Val Leu His Thr Ala Glu Ala Gly Gly Arg Ala 2120 2125 2130 Tyr Asn His Ala Leu Ser Glu Leu Pro Glu Thr Leu Glu Thr Leu 2135 2140 2145 Leu Leu Leu Thr Leu Leu Ala Thr Val Thr Gly Gly Ile Phe Leu 2150 2155 2160 Phe Leu Met Ser Gly Arg Gly Ile Gly Lys Met Thr Leu Gly Met 2165 2170 2175 Cys Cys Ile Ile Thr Ala Ser Ile Leu Leu Trp Tyr Ala Gln Ile 2180 2185 2190 Gln Pro His Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe Leu 2195 2200 2205 Ile Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln 2210 2215 2220 Asp Asn Gln Leu Thr Tyr Val Val Ile Ala Ile Leu Thr Val Val 2225 2230 2235 Ala Ala Thr Met Ala Asn Glu Met Gly Phe Leu Glu Lys Thr Lys 2240 2245 2250 Lys Asp Leu Gly Leu Gly Ser Ile Ala Thr Gln Gln Pro Glu Ser 2255 2260 2265 Asn Ile Leu Asp Ile Asp Leu Arg Pro Ala Ser Ala Trp Thr Leu 2270 2275 2280 Tyr Ala Val Ala Thr Thr Phe Val Thr Pro Met Leu Arg His Ser 2285 2290 2295 Ile Glu Asn Ser Ser Val Asn Val Ser Leu Thr Ala Ile Ala Asn 2300 2305 2310 Gln Ala Thr Val Leu Met Gly Leu Gly Lys Gly Trp Pro Leu Ser 2315 2320 2325 Lys Met Asp Ile Gly Val Pro Leu Leu Ala Ile Gly Cys Tyr Ser 2330 2335 2340 Gln Val Asn Pro Ile Thr Leu Thr Ala Ala Leu Leu Leu Leu Val 2345 2350 2355 Ala His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr 2360 2365 2370 Arg Glu Ala Gln Lys Arg Ala Ala Ala Gly Ile Met Lys Asn Pro 2375 2380 2385 Thr Val Asp Gly Ile Thr Val Ile Asp Leu Asp Pro Ile Pro Tyr 2390 2395 2400 Asp Pro Lys Phe Glu Lys Gln Leu Gly Gln Val Met Leu Leu Ile 2405 2410 2415 Leu Cys Val Thr Gln Val Leu Met Met Arg Thr Thr Trp Ala Leu 2420 2425 2430 Cys Glu Ala Leu Thr Leu Ala Thr Gly Pro Ile Ser Thr Leu Trp 2435 2440 2445 Glu Gly Asn Pro Gly Arg Phe Trp Asn Thr Thr Ile Ala Val Ser 2450 2455 2460 Met Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu 2465 2470 2475 Leu Phe Ser Ile Met Lys Asn Thr Thr Asn Thr Arg Arg Gly Thr 2480 2485 2490 Gly Asn Ile Gly Glu Thr Leu Gly Glu Lys Trp Lys Ser Arg Leu 2495 2500 2505 Asn Ala Leu Gly Lys Ser Glu Phe Gln Ile Tyr Lys Lys Ser Gly 2510 2515 2520 Ile Gln Glu Val Asp Arg Thr Leu Ala Lys Glu Gly Ile Lys Arg 2525 2530 2535 Gly Glu Thr Asp His His Ala Val Ser Arg Gly Ser Ala Lys Leu 2540 2545 2550 Arg Trp Phe Val Glu Arg Asn Met Val Thr Pro Glu Gly Lys Val 2555 2560 2565 Val Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Cys Gly 2570 2575 2580 Gly Leu Lys Asn Val Arg Glu Val Lys Gly Leu Thr Lys Gly Gly 2585 2590 2595 Pro Gly His Glu Glu Pro Ile Pro Met Ser Thr Tyr Gly Trp Asn 2600 2605 2610 Leu Val Arg Leu Gln Ser Gly Val Asp Val Phe Phe Ile Pro Pro 2615 2620 2625 Glu Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Pro 2630 2635 2640 Asn Pro Thr Val Glu Ala Gly Arg Thr Leu Arg Val Leu Asn Leu 2645 2650 2655 Val Glu Asn Trp Leu Asn Asn Asn Thr Gln Phe Cys Ile Lys Val 2660 2665 2670 Leu Asn Pro Tyr Met Pro Ser Val Ile Glu Lys Met Glu Ala Leu 2675 2680 2685 Gln Arg Lys Tyr Gly Gly Ala Leu Val Arg Asn Pro Leu Ser Arg 2690 2695 2700 Asn Ser Thr His Glu Met Tyr Trp Val Ser Asn Ala Ser Gly Asn 2705 2710 2715 Ile Val Ser Ser Val Asn Met Ile Ser Arg Met Leu Ile Asn Arg 2720 2725 2730 Phe Thr Met Arg His Lys Lys Ala Thr Tyr Glu Pro Asp Val Asp 2735 2740 2745 Leu Gly Ser Gly Thr Arg Asn Ile Gly Val Glu Ser Glu Ile Pro 2750 2755 2760 Asn Leu Asp Ile Ile Gly Lys Arg Ile Glu Lys Ile Lys Gln Glu 2765 2770 2775 His Glu Thr Ser Trp His Tyr Asp Gln Asp His Pro Tyr Lys Thr 2780 2785 2790 Trp Ala Tyr His Gly Ser Tyr Glu Thr Lys Gln Thr Gly Ser Ala 2795 2800 2805 Ser Ser Met Val Asn Gly Val Val Arg Leu Leu Thr Lys Pro Trp 2810 2815 2820 Asp Val Leu Pro Thr Val Thr Gln Met Ala Met Thr Asp Thr Thr 2825 2830 2835 Pro Phe Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg 2840 2845 2850 Thr Gln Glu Pro Lys Glu Gly Thr Lys Lys Leu Met Arg Ile Thr 2855 2860 2865 Ala Glu Trp Leu Trp Lys Glu Leu Gly Lys Lys Lys Thr Pro Arg 2870 2875 2880 Met Cys Thr Arg Glu Glu Phe Thr Arg Lys Val Arg Ser Asn Ala 2885 2890 2895 Ala Leu Gly Ala Ile Phe Thr Asp Glu Asn Lys Trp Lys Ser Ala 2900 2905 2910 Arg Glu Ala Val Glu Asp Ser Arg Phe Trp Glu Leu Val Asp Lys 2915 2920 2925 Glu Arg Asn Leu His Leu Glu Gly Lys Cys Glu Thr Cys Val Tyr 2930 2935 2940 Asn Met Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Lys 2945 2950 2955 Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg 2960 2965 2970 Phe Leu Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp 2975 2980 2985 Phe Ser Arg Glu Asn Ser Leu Ser Gly Val Glu Gly Glu Gly Leu 2990 2995 3000 His Lys Leu Gly Tyr Ile Leu Arg Asp Val Ser Lys Lys Glu Gly 3005 3010 3015 Gly Ala Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile 3020 3025 3030 Thr Leu Glu Asp Leu Lys Asn Glu Glu Met Val Thr Asn His Met 3035 3040 3045 Glu Gly Glu His Lys Lys Leu Ala Glu Ala Ile Phe Lys Leu Thr 3050 3055 3060 Tyr Gln Asn Lys Val Val Arg Val Gln Arg Pro Thr Pro Arg Gly 3065 3070 3075 Thr Val Met Asp Ile Ile Ser Arg Arg Asp Gln Arg Gly Ser Gly 3080 3085 3090 Gln Val Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Ala 3095 3100 3105 Gln Leu Ile Arg Gln Met Glu Gly Glu Gly Val Phe Lys Asn Ile 3110 3115 3120 Gln His Leu Thr Val Thr Glu Glu Val Ala Val Gln Asn Trp Leu 3125 3130 3135 Ala Arg Val Gly Arg Glu Arg Leu Ser Arg Met Ala Ile Ser Gly 3140 3145 3150 Asp Asp Cys Val Val Lys Pro Leu Asp Asp Arg Phe Ala Asn Ala 3155 3160 3165 Leu Thr Ala Leu Asn Asp Met Gly Lys Ile Arg Lys Asp Ile Gln 3170 3175 3180 Gln Trp Glu Pro Ser Lys Gly Trp Asn Asp Trp Thr Gln Val Pro 3185 3190 3195 Phe Cys Ser His His Phe His Glu Leu Ile Met Lys Asp Gly Arg 3200 3205 3210 Val Leu Val Val Pro Cys Arg Asn Gln Asp Glu Leu Ile Gly Arg 3215 3220 3225 Ala Arg Ile Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala 3230 3235 3240 Cys Leu Gly Lys Ser Tyr Ala Gln Met Trp Ser Leu Met Tyr Phe 3245 3250 3255 His Arg Arg Asp Leu Arg Leu Ala Ala Asn Ala Ile Cys Ser Ala 3260 3265 3270 Val Pro Ser His Trp Val Pro Thr Ser Arg Thr Thr Trp Ser Ile 3275 3280 3285 His Ala Lys His Glu Trp Met Thr Thr Glu Asp Met Leu Thr Val 3290 3295 3300 Trp Asn Arg Val Trp Ile Arg Glu Asn Pro Trp Met Glu Asp Lys 3305 3310 3315 Thr Pro Val Glu Ser Trp Glu Glu Ile Pro Tyr Leu Gly Lys Arg 3320 3325 3330 Glu Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ser Arg Ala 3335 3340 3345 Thr Trp Ala Lys Asn Ile Gln Ala Ala Ile Asn Gln Val Arg Ser 3350 3355 3360 Leu Ile Gly Asn Glu Glu Tyr Thr Asp Tyr Met Pro Ser Met Lys 3365 3370 3375 Arg Phe Arg Arg Glu Glu Glu Glu Ala Gly Val Leu Trp 3380 3385 3390 <210> 7 <211> 3390 <212> PRT <213> Dengue virus type 3 <400> 7 Met Asn Asn Gln Arg Lys Lys Thr Gly Lys Pro Ser Ile Asn Met Leu 1 5 10 15 Lys Arg Val Arg Asn Arg Val Ser Thr Gly Ser Gln Leu Ala Lys Arg 20 25 30 Phe Ser Arg Gly Leu Leu Asn Gly Gln Gly Pro Met Lys Leu Val Met 35 40 45 Ala Phe Ile Ala Phe Leu Arg Phe Leu Ala Ile Pro Pro Thr Ala Gly 50 55 60 Val Leu Ala Arg Trp Gly Thr Phe Lys Lys Ser Gly Ala Ile Lys Val 65 70 75 80 Leu Lys Gly Phe Lys Lys Glu Ile Ser Asn Met Leu Ser Ile Ile Asn 85 90 95 Lys Arg Lys Lys Thr Ser Leu Cys Leu Met Met Met Leu Pro Ala Thr 100 105 110 Leu Ala Phe His Leu Thr Ser Arg Asp Gly Glu Pro Arg Met Ile Val 115 120 125 Gly Lys Asn Glu Arg Gly Lys Ser Leu Leu Phe Lys Thr Ala Ser Gly 130 135 140 Ile Asn Met Cys Thr Leu Ile Ala Met Asp Leu Gly Glu Met Cys Asp 145 150 155 160 Asp Thr Val Thr Tyr Lys Cys Pro His Ile Thr Glu Val Glu Pro Glu 165 170 175 Asp Ile Asp Cys Trp Cys Asn Leu Thr Ser Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Asn Gln Ala Gly Glu His Arg Arg Asp Lys Arg Ser Val Ala 195 200 205 Leu Ala Pro His Val Gly Met Gly Leu Asp Thr Arg Thr Gln Thr Trp 210 215 220 Met Ser Ala Glu Gly Ala Trp Arg Gln Val Glu Lys Val Glu Thr Trp 225 230 235 240 Ala Leu Arg His Pro Gly Phe Thr Ile Leu Ala Leu Phe Leu Ala His 245 250 255 Tyr Ile Gly Thr Ser Leu Thr Gln Lys Val Val Ile Phe Ile Leu Leu 260 265 270 Met Leu Val Thr Pro Ser Met Thr Met Arg Cys Val Gly Val Gly Asn 275 280 285 Arg Asp Phe Val Glu Gly Leu Ser Gly Ala Thr Trp Val Asp Val Val 290 295 300 Leu Glu His Gly Gly Cys Val Thr Thr Met Ala Lys Asn Lys Pro Thr 305 310 315 320 Leu Asp Ile Glu Leu Gln Lys Thr Glu Ala Thr Gln Leu Ala Thr Leu 325 330 335 Arg Lys Leu Cys Ile Glu Gly Lys Ile Thr Asn Ile Thr Thr Asp Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Ala Ile Leu Pro Glu Glu Gln Asp Gln 355 360 365 Asn Tyr Val Cys Lys His Thr Tyr Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Gln 385 390 395 400 Cys Leu Glu Ser Ile Glu Gly Lys Val Val Gln His Glu Asn Leu Lys 405 410 415 Tyr Thr Val Ile Ile Thr Val His Thr Gly Asp Gln His Gln Val Gly 420 425 430 Asn Glu Thr Gln Gly Val Thr Ala Glu Ile Thr Pro Gln Ala Ser Thr 435 440 445 Ala Glu Ala Ile Leu Pro Glu Tyr Gly Thr Leu Gly Leu Glu Cys Ser 450 455 460 Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Ile Ser Leu Thr Met Lys 465 470 475 480 Asn Lys Ala Trp Met Val His Arg Gln Trp Phe Phe Asp Leu Pro Leu 485 490 495 Pro Trp Thr Ser Gly Ala Thr Ala Glu Thr Pro Thr Trp Asn Arg Lys 500 505 510 Glu Leu Leu Val Thr Phe Lys Asn Ala His Ala Lys Lys Gln Glu Val 515 520 525 Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu Thr Gly 530 535 540 Ala Thr Glu Ile Gln Thr Ser Gly Gly Thr Ser Ile Phe Ala Gly His 545 550 555 560 Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Glu Leu Lys Gly Met Ser 565 570 575 Tyr Ala Met Cys Leu Gly Ser Phe Val Leu Lys Lys Glu Val Ser Glu 580 585 590 Thr Gln His Gly Thr Ile Leu Ile Lys Val Glu Tyr Lys Gly Lys Asp 595 600 605 Ala Pro Cys Lys Ile Pro Phe Ser Thr Glu Asp Gly Gln Gly Lys Ala 610 615 620 His Asn Gly Arg Leu Ile Thr Ala Asn Pro Val Val Thr Lys Lys Glu 625 630 635 640 Glu Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser Asn Ile 645 650 655 Val Ile Gly Ile Gly Asp Lys Ala Leu Lys Ile Asn Trp Tyr Arg Lys 660 665 670 Gly Ser Ser Ile Gly Lys Met Phe Glu Ala Thr Ala Arg Gly Ala Arg 675 680 685 Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly 690 695 700 Gly Val Leu Asn Ser Leu Gly Lys Met Val His Gln Ile Phe Gly Ser 705 710 715 720 Ala Tyr Thr Ala Leu Phe Gly Gly Val Ser Trp Met Met Lys Ile Gly 725 730 735 Ile Gly Val Leu Leu Thr Trp Ile Gly Leu Asn Ser Lys Asn Thr Ser 740 745 750 Met Ser Phe Ser Cys Ile Ala Ile Gly Ile Ile Thr Leu Tyr Leu Gly 755 760 765 Ala Val Val Gln Ala Asp Met Gly Cys Val Ile Asn Trp Lys Gly Lys 770 775 780 Glu Leu Lys Cys Gly Ser Gly Ile Phe Val Thr Asn Glu Val His Thr 785 790 795 800 Trp Thr Glu Gln Tyr Lys Phe Gln Ala Asp Ser Pro Lys Arg Leu Ala 805 810 815 Thr Ala Ile Ala Gly Ala Trp Glu Asn Gly Val Cys Gly Ile Arg Ser 820 825 830 Thr Thr Arg Met Glu Asn Leu Leu Trp Lys Gln Ile Ala Asn Glu Leu 835 840 845 Asn Tyr Ile Leu Trp Glu Asn Asn Ile Lys Leu Thr Val Val Val Gly 850 855 860 Asp Ile Thr Gly Val Leu Glu Gln Gly Lys Arg Thr Leu Thr Pro Gln 865 870 875 880 Pro Met Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala Lys Ile 885 890 895 Val Thr Ala Glu Thr Gln Asn Ser Ser Phe Ile Ile Asp Gly Pro Ser 900 905 910 Thr Pro Glu Cys Pro Ser Ala Ser Arg Ala Trp Asn Val Trp Glu Val 915 920 925 Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu Lys Leu 930 935 940 Arg Glu Val Tyr Thr Gln Leu Cys Asp His Arg Leu Met Ser Ala Ala 945 950 955 960 Val Lys Asp Glu Arg Ala Val His Ala Asp Met Gly Tyr Trp Ile Glu 965 970 975 Ser Gln Lys Asn Gly Ser Trp Lys Leu Glu Lys Ala Ser Phe Ile Glu 980 985 990 Val Lys Thr Cys Thr Trp Pro Lys Ser His Thr Leu Trp Ser Asn Gly 995 1000 1005 Val Leu Glu Ser Asp Met Ile Ile Pro Lys Ser Leu Ala Gly Pro 1010 1015 1020 Ile Ser Gln His Asn His Arg Pro Gly Tyr His Thr Gln Thr Ala 1025 1030 1035 Gly Pro Trp His Leu Gly Lys Leu Glu Leu Asp Phe Asn Tyr Cys 1040 1045 1050 Glu Gly Thr Thr Val Val Ile Ser Glu Asn Cys Gly Thr Arg Gly 1055 1060 1065 Pro Ser Leu Arg Thr Thr Thr Val Ser Gly Lys Leu Ile His Glu 1070 1075 1080 Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Tyr Met Gly 1085 1090 1095 Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Ile Asn Glu 1100 1105 1110 Lys Glu Glu Asn Met Val Lys Ser Leu Ala Ser Ala Gly Ser Gly 1115 1120 1125 Lys Val Asp Asn Phe Thr Met Gly Val Leu Cys Leu Ala Ile Leu 1130 1135 1140 Phe Glu Glu Val Met Arg Gly Lys Phe Gly Lys Lys His Met Ile 1145 1150 1155 Ala Gly Val Leu Phe Thr Phe Val Leu Leu Leu Ser Gly Gln Ile 1160 1165 1170 Thr Trp Arg Asp Met Ala His Thr Leu Ile Met Ile Gly Ser Asn 1175 1180 1185 Ala Ser Asp Arg Met Gly Met Gly Val Thr Tyr Leu Ala Leu Ile 1190 1195 1200 Ala Thr Phe Lys Ile Gln Pro Leu Leu Ala Leu Gly Phe Phe Leu 1205 1210 1215 Arg Lys Leu Thr Ser Arg Glu Asn Leu Leu Leu Gly Val Gly Leu 1220 1225 1230 Ala Met Ala Ala Thr Leu Arg Leu Pro Glu Asp Ile Glu Gln Met 1235 1240 1245 Ala Asn Gly Ile Ala Leu Gly Leu Met Ala Leu Lys Leu Ile Thr 1250 1255 1260 Gln Phe Glu Thr Tyr Gln Leu Trp Thr Ala Leu Val Ser Leu Thr 1265 1270 1275 Cys Ser Asn Thr Ile Phe Thr Leu Thr Val Ala Trp Arg Thr Ala 1280 1285 1290 Thr Leu Ile Leu Ala Gly Ile Ser Leu Leu Pro Val Cys Gln Ser 1295 1300 1305 Ser Ser Met Arg Lys Thr Asp Trp Leu Pro Met Thr Val Ala Ala 1310 1315 1320 Met Gly Ala Gln Pro Leu Pro Leu Phe Ile Phe Ser Leu Lys Asp 1325 1330 1335 Thr Leu Lys Arg Arg Ser Trp Pro Leu Asn Glu Gly Val Met Ala 1340 1345 1350 Val Gly Leu Val Ser Ile Leu Ala Ser Ser Leu Leu Arg Asn Asp 1355 1360 1365 Val Pro Met Ala Gly Pro Leu Val Ala Gly Gly Leu Leu Ile Ala 1370 1375 1380 Cys Tyr Val Ile Thr Gly Thr Ser Ala Asp Leu Thr Val Glu Lys 1385 1390 1395 Ala Ala Asp Val Thr Trp Glu Glu Glu Ala Glu Gln Thr Gly Val 1400 1405 1410 Ser His Asn Leu Met Val Thr Val Asp Asp Asp Gly Thr Met Arg 1415 1420 1425 Ile Lys Asp Asp Glu Thr Glu Asn Ile Leu Thr Val Leu Leu Lys 1430 1435 1440 Thr Ala Leu Leu Ile Val Ser Gly Ile Phe Pro Tyr Ser Ile Pro 1445 1450 1455 Ala Thr Leu Leu Val Trp His Thr Trp Gln Lys Gln Thr Gln Arg 1460 1465 1470 Ser Gly Val Leu Trp Asp Val Pro Ser Pro Pro Glu Thr Gln Lys 1475 1480 1485 Ala Glu Leu Glu Glu Gly Val Tyr Arg Ile Lys Gln Gln Gly Ile 1490 1495 1500 Phe Gly Lys Thr Gln Val Gly Val Gly Val Gln Lys Glu Gly Val 1505 1510 1515 Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu Thr His 1520 1525 1530 Asn Gly Lys Arg Leu Glu Pro Asn Trp Ala Ser Val Lys Lys Asp 1535 1540 1545 Leu Ile Ser Tyr Gly Gly Gly Trp Arg Leu Ser Ala Gln Trp Lys 1550 1555 1560 Lys Gly Glu Glu Val Gln Val Ile Ala Val Glu Pro Gly Lys Asn 1565 1570 1575 Pro Lys Asn Phe Gln Thr Met Pro Gly Ile Phe Gln Thr Thr Thr 1580 1585 1590 Gly Glu Ile Gly Ala Ile Ala Leu Asp Phe Lys Pro Gly Thr Ser 1595 1600 1605 Gly Ser Pro Ile Ile Asn Arg Glu Gly Lys Val Val Gly Leu Tyr 1610 1615 1620 Gly Asn Gly Val Val Thr Lys Asn Gly Gly Tyr Val Ser Gly Ile 1625 1630 1635 Ala Gln Thr Asn Ala Glu Pro Asp Gly Pro Thr Pro Glu Leu Glu 1640 1645 1650 Glu Glu Met Phe Lys Lys Arg Asn Leu Thr Ile Met Asp Leu His 1655 1660 1665 Pro Gly Ser Gly Lys Thr Arg Lys Tyr Leu Pro Ala Ile Val Arg 1670 1675 1680 Glu Ala Ile Lys Arg Arg Leu Arg Thr Leu Ile Leu Ala Pro Thr 1685 1690 1695 Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Lys Gly Leu Pro 1700 1705 1710 Ile Arg Tyr Gln Thr Thr Ala Thr Lys Ser Glu His Thr Gly Arg 1715 1720 1725 Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met Arg Leu 1730 1735 1740 Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Leu Ile Ile Met Asp 1745 1750 1755 Glu Ala His Phe Thr Asp Pro Ala Ser Ile Ala Ala Arg Gly Tyr 1760 1765 1770 Ile Ser Thr Arg Val Gly Met Gly Glu Ala Ala Ala Ile Phe Met 1775 1780 1785 Thr Ala Thr Pro Pro Gly Thr Ala Asp Ala Phe Pro Gln Ser Asn 1790 1795 1800 Ala Pro Ile Gln Asp Glu Glu Arg Asp Ile Pro Glu Arg Ser Trp 1805 1810 1815 Asn Ser Gly Asn Glu Trp Ile Thr Asp Phe Val Gly Lys Thr Val 1820 1825 1830 Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Asn Cys 1835 1840 1845 Leu Arg Lys Asn Gly Lys Lys Val Ile Gln Leu Ser Arg Lys Thr 1850 1855 1860 Phe Asp Thr Glu Tyr Gln Lys Thr Lys Leu Asn Asp Trp Asp Phe 1865 1870 1875 Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys Ala 1880 1885 1890 Asp Arg Val Ile Asp Pro Arg Arg Cys Leu Lys Pro Val Ile Leu 1895 1900 1905 Thr Asp Gly Pro Glu Arg Val Ile Leu Ala Gly Pro Met Pro Val 1910 1915 1920 Thr Val Ala Ser Ala Ala Gln Arg Arg Gly Arg Val Gly Arg Asn 1925 1930 1935 Pro Gln Lys Glu Asn Asp Gln Tyr Ile Phe Met Gly Gln Pro Leu 1940 1945 1950 Asn Asn Asp Glu Asp His Ala His Trp Thr Glu Ala Lys Met Leu 1955 1960 1965 Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ala Leu Phe 1970 1975 1980 Glu Pro Glu Arg Glu Lys Ser Ala Ala Ile Asp Gly Glu Tyr Arg 1985 1990 1995 Leu Lys Gly Glu Ser Arg Lys Thr Phe Val Glu Leu Met Arg Arg 2000 2005 2010 Gly Asp Leu Pro Val Trp Leu Ala His Lys Val Ala Ser Glu Gly 2015 2020 2025 Ile Lys Tyr Thr Asp Arg Lys Trp Cys Phe Asp Gly Glu Arg Asn 2030 2035 2040 Asn Gln Ile Leu Glu Glu Asn Met Asp Val Glu Ile Trp Thr Lys 2045 2050 2055 Glu Gly Glu Lys Lys Lys Leu Arg Pro Arg Trp Leu Asp Ala Arg 2060 2065 2070 Thr Tyr Ser Asp Pro Leu Ala Leu Lys Glu Phe Lys Asp Phe Ala 2075 2080 2085 Ala Gly Arg Lys Ser Ile Ala Leu Asp Leu Val Thr Glu Ile Gly 2090 2095 2100 Arg Val Pro Ser His Leu Ala His Arg Thr Arg Asn Ala Leu Asp 2105 2110 2115 Asn Leu Val Met Leu His Thr Ser Glu His Gly Gly Arg Ala Tyr 2120 2125 2130 Arg His Ala Val Glu Glu Leu Pro Glu Thr Met Glu Thr Leu Leu 2135 2140 2145 Leu Leu Gly Leu Met Ile Leu Leu Thr Gly Gly Ala Met Leu Phe 2150 2155 2160 Leu Ile Ser Gly Lys Gly Ile Gly Lys Thr Ser Ile Gly Leu Ile 2165 2170 2175 Cys Val Ile Ala Ser Ser Gly Met Leu Trp Met Ala Asp Val Pro 2180 2185 2190 Leu Gln Trp Ile Ala Ser Ala Ile Val Leu Glu Phe Phe Met Met 2195 2200 2205 Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln Asp 2210 2215 2220 Asn Gln Leu Ala Tyr Val Val Ile Gly Ile Leu Thr Leu Ala Ala 2225 2230 2235 Ile Val Ala Ala Asn Glu Met Gly Leu Leu Glu Thr Thr Lys Arg 2240 2245 2250 Asp Leu Gly Met Ser Lys Glu Pro Gly Val Val Ser Pro Thr Ser 2255 2260 2265 Tyr Leu Asp Val Asp Leu His Pro Ala Ser Ala Trp Thr Leu Tyr 2270 2275 2280 Ala Val Ala Thr Thr Val Ile Thr Pro Met Leu Arg His Thr Ile 2285 2290 2295 Glu Asn Ser Thr Ala Asn Val Ser Leu Ala Ala Ile Ala Asn Gln 2300 2305 2310 Ala Val Val Leu Met Gly Leu Asp Lys Gly Trp Pro Ile Ser Lys 2315 2320 2325 Met Asp Leu Gly Val Pro Leu Leu Ala Leu Gly Cys Tyr Ser Gln 2330 2335 2340 Val Asn Pro Leu Thr Leu Ala Ala Ala Val Leu Leu Leu Val Thr 2345 2350 2355 His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr Arg 2360 2365 2370 Glu Ala Gln Lys Arg Thr Ala Ala Gly Ile Met Lys Asn Pro Thr 2375 2380 2385 Val Asp Gly Ile Met Thr Ile Asp Leu Asp Pro Val Ile Tyr Asp 2390 2395 2400 Ser Lys Phe Glu Lys Gln Leu Gly Gln Val Met Leu Leu Val Leu 2405 2410 2415 Cys Ala Val Gln Leu Leu Leu Met Arg Thr Ser Trp Ala Leu Cys 2420 2425 2430 Glu Val Leu Thr Leu Ala Thr Gly Pro Ile Thr Thr Leu Trp Glu 2435 2440 2445 Gly Ser Pro Gly Lys Phe Trp Asn Thr Thr Ile Ala Val Ser Met 2450 2455 2460 Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu Ala 2465 2470 2475 Phe Ser Ile Met Lys Ser Val Gly Thr Gly Lys Arg Gly Thr Gly 2480 2485 2490 Ser Gln Gly Glu Thr Leu Gly Glu Lys Trp Lys Lys Lys Leu Asn 2495 2500 2505 Gln Leu Ser Arg Lys Glu Phe Asp Leu Tyr Lys Lys Ser Gly Ile 2510 2515 2520 Thr Glu Val Asp Arg Thr Glu Ala Lys Glu Gly Leu Lys Arg Gly 2525 2530 2535 Glu Ile Thr His His Ala Val Ser Arg Gly Ser Ala Lys Leu Gln 2540 2545 2550 Trp Phe Val Glu Arg Asn Met Val Ile Pro Glu Gly Arg Val Ile 2555 2560 2565 Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Cys Ala Gly 2570 2575 2580 Leu Lys Lys Val Thr Glu Val Arg Gly Tyr Thr Lys Gly Gly Pro 2585 2590 2595 Gly His Glu Glu Pro Val Pro Met Ser Thr Tyr Gly Trp Asn Ile 2600 2605 2610 Val Lys Leu Met Ser Gly Lys Asp Val Phe Tyr Leu Pro Pro Glu 2615 2620 2625 Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Pro Ser 2630 2635 2640 Pro Thr Val Glu Glu Ser Arg Thr Ile Arg Val Leu Lys Met Val 2645 2650 2655 Glu Pro Trp Leu Lys Asn Asn Gln Phe Cys Ile Lys Val Leu Asn 2660 2665 2670 Pro Tyr Met Pro Thr Val Ile Glu His Leu Glu Arg Leu Gln Arg 2675 2680 2685 Lys His Gly Gly Met Leu Val Arg Asn Pro Leu Ser Arg Asn Ser 2690 2695 2700 Thr His Glu Met Tyr Trp Ile Ser Asn Gly Thr Gly Asn Ile Val 2705 2710 2715 Ser Ser Val Asn Met Val Ser Arg Leu Leu Leu Asn Arg Phe Thr 2720 2725 2730 Met Thr His Arg Arg Pro Thr Ile Glu Lys Asp Val Asp Leu Gly 2735 2740 2745 Ala Gly Thr Arg His Val Asn Ala Glu Pro Glu Thr Pro Asn Met 2750 2755 2760 Asp Val Ile Gly Glu Arg Ile Lys Arg Ile Lys Glu Glu His Ser 2765 2770 2775 Ser Thr Trp His Tyr Asp Asp Glu Asn Pro Tyr Lys Thr Trp Ala 2780 2785 2790 Tyr His Gly Ser Tyr Glu Val Lys Ala Thr Gly Ser Ala Ser Ser 2795 2800 2805 Met Ile Asn Gly Val Val Lys Leu Leu Thr Lys Pro Trp Asp Val 2810 2815 2820 Val Pro Met Val Thr Gln Met Ala Met Thr Asp Thr Thr Pro Phe 2825 2830 2835 Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg Thr Pro 2840 2845 2850 Arg Pro Met Pro Gly Thr Arg Lys Val Met Glu Ile Thr Ala Glu 2855 2860 2865 Trp Leu Trp Arg Thr Leu Gly Arg Asn Lys Arg Pro Arg Leu Cys 2870 2875 2880 Thr Arg Glu Glu Phe Thr Lys Lys Val Arg Thr Asn Ala Ala Met 2885 2890 2895 Gly Ala Val Phe Thr Glu Glu Asn Gln Trp Asp Ser Ala Arg Ala 2900 2905 2910 Ala Val Glu Asp Glu Glu Phe Trp Lys Leu Val Asp Arg Glu Arg 2915 2920 2925 Glu Leu His Lys Leu Gly Lys Cys Gly Ser Cys Val Tyr Asn Met 2930 2935 2940 Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Lys Ala Lys 2945 2950 2955 Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg Tyr Leu 2960 2965 2970 Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp Phe Ser 2975 2980 2985 Arg Glu Asn Ser Tyr Ser Gly Val Glu Gly Glu Gly Leu His Lys 2990 2995 3000 Leu Gly Tyr Ile Leu Arg Asp Ile Ser Lys Ile Pro Gly Gly Ala 3005 3010 3015 Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile Thr Glu 3020 3025 3030 Asp Asp Leu His Asn Glu Glu Lys Ile Thr Gln Gln Met Asp Pro 3035 3040 3045 Glu His Arg Gln Leu Ala Asn Ala Ile Phe Lys Leu Thr Tyr Gln 3050 3055 3060 Asn Lys Val Val Lys Val Gln Arg Pro Thr Pro Lys Gly Thr Val 3065 3070 3075 Met Asp Ile Ile Ser Arg Lys Asp Gln Arg Gly Ser Gly Gln Val 3080 3085 3090 Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Ala Gln Leu 3095 3100 3105 Ile Arg Gln Met Glu Gly Glu Gly Val Leu Ser Lys Ala Asp Leu 3110 3115 3120 Glu Asn Pro His Pro Leu Glu Lys Lys Ile Thr Gln Trp Leu Glu 3125 3130 3135 Thr Lys Gly Val Glu Arg Leu Lys Arg Met Ala Ile Ser Gly Asp 3140 3145 3150 Asp Cys Val Val Lys Pro Ile Asp Asp Arg Phe Ala Asn Ala Leu 3155 3160 3165 Leu Ala Leu Asn Asp Met Gly Lys Val Arg Lys Asp Ile Pro Gln 3170 3175 3180 Trp Gln Pro Ser Lys Gly Trp His Asp Trp Gln Gln Val Pro Phe 3185 3190 3195 Cys Ser His His Phe His Glu Leu Ile Met Lys Asp Gly Arg Lys 3200 3205 3210 Leu Val Val Pro Cys Arg Pro Gln Asp Glu Leu Ile Gly Arg Ala 3215 3220 3225 Arg Ile Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala Cys 3230 3235 3240 Leu Gly Lys Ala Tyr Ala Gln Met Trp Ala Leu Met Tyr Phe His 3245 3250 3255 Arg Arg Asp Leu Arg Leu Ala Ser Asn Ala Ile Cys Ser Ala Val 3260 3265 3270 Pro Val His Trp Val Pro Thr Ser Arg Thr Thr Trp Ser Ile His 3275 3280 3285 Ala His His Gln Trp Met Thr Thr Glu Asp Met Leu Thr Val Trp 3290 3295 3300 Asn Arg Val Trp Ile Glu Asp Asn Pro Trp Met Glu Asp Lys Thr 3305 3310 3315 Pro Val Thr Thr Trp Glu Asp Val Pro Tyr Leu Gly Lys Arg Glu 3320 3325 3330 Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ser Arg Ala Thr 3335 3340 3345 Trp Ala Gln Asn Ile Leu Thr Ala Ile Gln Gln Val Arg Ser Leu 3350 3355 3360 Ile Gly Asn Glu Glu Phe Leu Asp Tyr Met Pro Ser Met Lys Arg 3365 3370 3375 Phe Arg Lys Glu Glu Glu Ser Glu Gly Ala Ile Trp 3380 3385 3390 <210> 8 <211> 3387 <212> PRT <213> Dengue virus type 4 <220> <221> VARIANT <222> (742)..(742) <223> Xaa stands for Val or Met <220> <221> VARIANT <222> (1027)..(1027) <223> Xaa stands for Gln or His <220> <221> VARIANT <222> (1628)..(1628) <223> Xaa stands for Val or Leu <220> <221> VARIANT <222> (2286)..(2286) <223> Xaa stands for Phe or Ile <220> <221> VARIANT <222> (2482)..(2482) <223> Xaa stands for Val or Ala <220> <221> VARIANT <222> (2508)..(2508) <223> Xaa stands for Tyr or Asp <400> 8 Met Asn Gln Arg Lys Lys Val Val Arg Pro Pro Phe Asn Met Leu Lys 1 5 10 15 Arg Glu Arg Asn Arg Val Ser Thr Pro Gln Gly Leu Val Lys Arg Phe 20 25 30 Ser Thr Gly Leu Phe Ser Gly Lys Gly Pro Leu Arg Met Val Leu Ala 35 40 45 Phe Ile Thr Phe Leu Arg Val Leu Ser Ile Pro Pro Thr Ala Gly Ile 50 55 60 Leu Lys Arg Trp Gly Gln Leu Lys Lys Asn Lys Ala Ile Arg Ile Leu 65 70 75 80 Ile Gly Phe Arg Lys Glu Ile Gly Arg Met Leu Asn Ile Leu Asn Gly 85 90 95 Arg Lys Arg Ser Thr Ile Thr Leu Leu Cys Leu Ile Pro Thr Val Met 100 105 110 Ala Phe His Leu Ser Thr Arg Asp Gly Glu Pro Leu Met Ile Val Ala 115 120 125 Lys His Glu Arg Gly Arg Pro Leu Leu Phe Lys Thr Thr Glu Gly Ile 130 135 140 Asn Lys Cys Thr Leu Ile Ala Met Asp Leu Gly Glu Met Cys Glu Asp 145 150 155 160 Thr Val Thr Tyr Lys Cys Pro Leu Leu Val Asn Thr Glu Pro Glu Asp 165 170 175 Ile Asp Cys Trp Cys Asn Leu Thr Ser Thr Trp Val Met Tyr Gly Thr 180 185 190 Cys Thr Gln Ser Gly Glu Arg Arg Arg Glu Lys Arg Ser Val Ala Leu 195 200 205 Thr Pro His Ser Gly Met Gly Leu Glu Thr Arg Ala Glu Thr Trp Met 210 215 220 Ser Ser Glu Gly Ala Trp Lys His Ala Gln Arg Val Glu Ser Trp Ile 225 230 235 240 Leu Arg Asn Pro Gly Phe Ala Leu Leu Ala Gly Phe Met Ala Tyr Met 245 250 255 Ile Gly Gln Thr Gly Ile Gln Arg Thr Val Phe Phe Val Leu Met Met 260 265 270 Leu Val Ala Pro Ser Tyr Gly Met Arg Cys Val Gly Val Gly Asn Arg 275 280 285 Asp Phe Val Glu Gly Val Ser Gly Gly Ala Trp Val Asp Leu Val Leu 290 295 300 Glu His Gly Gly Cys Val Thr Thr Met Ala Gln Gly Lys Pro Thr Leu 305 310 315 320 Asp Phe Glu Leu Thr Lys Thr Thr Ala Lys Glu Val Ala Leu Leu Arg 325 330 335 Thr Tyr Cys Ile Glu Ala Ser Ile Ser Asn Ile Thr Thr Ala Thr Arg 340 345 350 Cys Pro Thr Gln Gly Glu Pro Tyr Leu Lys Glu Glu Gln Asp Gln Gln 355 360 365 Tyr Ile Cys Arg Arg Asp Val Val Asp Arg Gly Trp Gly Asn Gly Cys 370 375 380 Gly Leu Phe Gly Lys Gly Gly Val Val Thr Cys Ala Lys Phe Ser Cys 385 390 395 400 Ser Gly Lys Ile Thr Gly Asn Leu Val Gln Ile Glu Asn Leu Glu Tyr 405 410 415 Thr Val Val Val Thr Val His Asn Gly Asp Thr His Ala Val Gly Asn 420 425 430 Asp Thr Ser Asn His Gly Val Thr Ala Thr Ile Thr Pro Arg Ser Pro 435 440 445 Ser Val Glu Val Lys Leu Pro Asp Tyr Gly Glu Leu Thr Leu Asp Cys 450 455 460 Glu Pro Arg Ser Gly Ile Asp Phe Asn Glu Met Ile Leu Met Lys Met 465 470 475 480 Lys Lys Lys Thr Trp Leu Val His Lys Gln Trp Phe Leu Asp Leu Pro 485 490 495 Leu Pro Trp Thr Ala Gly Ala Asp Thr Ser Glu Val His Trp Asn Tyr 500 505 510 Lys Glu Arg Met Val Thr Phe Lys Val Pro His Ala Lys Arg Gln Asp 515 520 525 Val Thr Val Leu Gly Ser Gln Glu Gly Ala Met His Ser Ala Leu Ala 530 535 540 Gly Ala Thr Glu Val Asp Ser Gly Asp Gly Asn His Met Phe Ala Gly 545 550 555 560 His Leu Lys Cys Lys Val Arg Met Glu Lys Leu Arg Ile Lys Gly Met 565 570 575 Ser Tyr Thr Met Cys Ser Gly Lys Phe Ser Ile Asp Lys Glu Met Ala 580 585 590 Glu Thr Gln His Gly Thr Thr Val Val Lys Val Lys Tyr Glu Gly Ala 595 600 605 Gly Ala Pro Cys Lys Val Pro Ile Glu Ile Arg Asp Val Asn Lys Lys 610 615 620 Lys Val Val Gly Arg Ile Ile Ser Ser Thr Pro Leu Ala Glu Asn Thr 625 630 635 640 Asn Ser Ala Thr Asn Ile Glu Leu Glu Pro Pro Phe Gly Asp Ser Tyr 645 650 655 Ile Val Ile Gly Val Gly Asn Ser Ala Leu Thr Leu His Trp Phe Arg 660 665 670 Lys Gly Ser Ser Ile Gly Lys Met Phe Glu Ser Thr Tyr Arg Gly Ala 675 680 685 Lys Arg Met Ala Ile Leu Gly Glu Thr Ala Trp Asp Phe Gly Ser Val 690 695 700 Gly Gly Leu Phe Thr Ser Leu Gly Lys Ala Val His Gln Val Phe Gly 705 710 715 720 Ser Val Tyr Thr Thr Met Phe Gly Gly Val Ser Trp Met Ile Arg Ile 725 730 735 Leu Ile Gly Phe Leu Xaa Leu Trp Ile Gly Thr Asn Ser Arg Asn Thr 740 745 750 Ser Met Ala Met Thr Cys Ile Ala Val Gly Gly Ile Thr Leu Phe Leu 755 760 765 Gly Phe Thr Val Gln Ala Asp Met Gly Cys Val Val Ser Trp Ser Gly 770 775 780 Lys Glu Leu Lys Cys Gly Ser Gly Ile Phe Val Val Asp Asn Val His 785 790 795 800 Thr Trp Thr Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ala Arg Leu 805 810 815 Ala Ser Ala Ile Leu Asn Ala His Lys Asp Gly Val Cys Gly Ile Arg 820 825 830 Ser Thr Thr Arg Leu Glu Asn Val Met Trp Lys Gln Ile Thr Asn Glu 835 840 845 Leu Asn Tyr Val Leu Trp Glu Gly Gly His Asp Leu Thr Val Val Ala 850 855 860 Gly Asp Val Lys Gly Val Leu Thr Lys Gly Lys Arg Ala Leu Thr Pro 865 870 875 880 Pro Val Asn Asp Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala Lys 885 890 895 Ile Phe Thr Pro Glu Ala Arg Asn Ser Thr Phe Leu Ile Asp Gly Pro 900 905 910 Asp Thr Ser Glu Cys Pro Asn Glu Arg Arg Ala Trp Asn Phe Leu Glu 915 920 925 Val Glu Asp Tyr Gly Phe Gly Met Phe Thr Thr Asn Ile Trp Met Lys 930 935 940 Phe Arg Glu Gly Ser Ser Glu Val Cys Asp His Arg Leu Met Ser Ala 945 950 955 960 Ala Ile Lys Asp Gln Lys Ala Val His Ala Asp Met Gly Tyr Trp Ile 965 970 975 Glu Ser Ser Lys Asn Gln Thr Trp Gln Ile Glu Lys Ala Ser Leu Ile 980 985 990 Glu Val Lys Thr Cys Leu Trp Pro Lys Thr His Thr Leu Trp Ser Asn 995 1000 1005 Gly Val Leu Glu Ser Gln Met Leu Ile Pro Lys Ser Tyr Ala Gly 1010 1015 1020 Pro Phe Ser Xaa His Asn Tyr Arg Gln Gly Tyr Ala Thr Gln Thr 1025 1030 1035 Val Gly Pro Trp His Leu Gly Lys Leu Glu Ile Asp Phe Gly Glu 1040 1045 1050 Cys Pro Gly Thr Thr Val Ala Ile Gln Glu Asp Cys Asp His Arg 1055 1060 1065 Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys Leu Val Thr 1070 1075 1080 Gln Trp Cys Cys Arg Ser Cys Thr Met Pro Pro Leu Arg Phe Leu 1085 1090 1095 Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu Ser 1100 1105 1110 Glu Lys Glu Glu Asn Met Val Lys Ser Gln Val Thr Ala Gly Gln 1115 1120 1125 Gly Thr Ser Glu Thr Phe Ser Met Gly Leu Leu Cys Leu Thr Leu 1130 1135 1140 Phe Val Glu Glu Cys Leu Arg Arg Arg Val Thr Arg Lys His Met 1145 1150 1155 Ile Leu Val Val Val Ile Thr Leu Cys Ala Ile Ile Leu Gly Gly 1160 1165 1170 Leu Thr Trp Met Asp Leu Leu Arg Ala Leu Ile Met Leu Gly Asp 1175 1180 1185 Thr Met Ser Gly Arg Ile Gly Gly Gln Ile His Leu Ala Ile Met 1190 1195 1200 Ala Val Phe Lys Met Ser Pro Gly Tyr Val Leu Gly Val Phe Leu 1205 1210 1215 Arg Lys Leu Thr Ser Arg Glu Thr Ala Leu Met Val Ile Gly Met 1220 1225 1230 Ala Met Thr Thr Val Leu Ser Ile Pro His Asp Leu Met Glu Leu 1235 1240 1245 Ile Asp Gly Ile Ser Leu Gly Leu Ile Leu Leu Lys Ile Val Thr 1250 1255 1260 His Phe Asp Asn Thr Gln Val Gly Thr Leu Ala Leu Ser Leu Thr 1265 1270 1275 Phe Ile Arg Ser Thr Met Pro Leu Val Met Ala Trp Arg Thr Ile 1280 1285 1290 Met Ala Val Leu Phe Val Val Thr Leu Ile Pro Leu Cys Arg Thr 1295 1300 1305 Ser Cys Leu Gln Lys Gln Ser His Trp Val Glu Ile Thr Ala Leu 1310 1315 1320 Ile Leu Gly Ala Gln Ala Leu Pro Val Tyr Leu Met Thr Leu Met 1325 1330 1335 Lys Gly Ala Ser Arg Arg Ser Trp Pro Leu Asn Glu Gly Ile Met 1340 1345 1350 Ala Val Gly Leu Val Ser Leu Leu Gly Ser Ala Leu Leu Lys Asn 1355 1360 1365 Asp Val Pro Leu Ala Gly Pro Met Val Ala Gly Gly Leu Leu Leu 1370 1375 1380 Ala Ala Tyr Val Met Ser Gly Ser Ser Ala Asp Leu Ser Leu Glu 1385 1390 1395 Lys Ala Ala Asn Val Gln Trp Asp Glu Met Ala Asp Ile Thr Gly 1400 1405 1410 Ser Ser Pro Ile Ile Glu Val Lys Gln Asp Glu Asp Gly Ser Phe 1415 1420 1425 Ser Ile Arg Asp Val Glu Glu Thr Asn Met Ile Thr Leu Leu Val 1430 1435 1440 Lys Leu Ala Leu Ile Thr Val Ser Gly Leu Tyr Pro Leu Ala Ile 1445 1450 1455 Pro Val Thr Met Thr Leu Trp Tyr Met Trp Gln Val Lys Thr Gln 1460 1465 1470 Arg Ser Gly Ala Leu Trp Asp Val Pro Ser Pro Ala Ala Thr Gln 1475 1480 1485 Lys Ala Ala Leu Ser Glu Gly Val Tyr Arg Ile Met Gln Arg Gly 1490 1495 1500 Leu Phe Gly Lys Thr Gln Val Gly Val Gly Ile His Met Glu Gly 1505 1510 1515 Val Phe His Thr Met Trp His Val Thr Arg Gly Ser Val Ile Cys 1520 1525 1530 His Glu Thr Gly Arg Leu Glu Pro Ser Trp Ala Asp Val Arg Asn 1535 1540 1545 Asp Met Ile Ser Tyr Gly Gly Gly Trp Arg Leu Gly Asp Lys Trp 1550 1555 1560 Asp Lys Glu Glu Asp Val Gln Val Leu Ala Ile Glu Pro Gly Lys 1565 1570 1575 Asn Pro Lys His Val Gln Thr Lys Pro Gly Leu Phe Lys Thr Leu 1580 1585 1590 Thr Gly Glu Ile Gly Ala Val Thr Leu Asp Phe Lys Pro Gly Thr 1595 1600 1605 Ser Gly Ser Pro Ile Ile Asn Arg Lys Gly Lys Val Ile Gly Leu 1610 1615 1620 Tyr Gly Asn Gly Xaa Val Thr Lys Ser Gly Asp Tyr Val Ser Ala 1625 1630 1635 Ile Thr Gln Ala Glu Arg Ile Gly Glu Pro Asp Tyr Glu Val Asp 1640 1645 1650 Glu Asp Ile Phe Arg Lys Lys Arg Leu Thr Ile Met Asp Leu His 1655 1660 1665 Pro Gly Ala Gly Lys Thr Lys Arg Ile Leu Pro Ser Ile Val Arg 1670 1675 1680 Glu Ala Leu Lys Arg Arg Leu Arg Thr Leu Ile Leu Ala Pro Thr 1685 1690 1695 Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu Pro 1700 1705 1710 Ile Arg Tyr Gln Thr Pro Ala Val Lys Ser Glu His Thr Gly Arg 1715 1720 1725 Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Thr Arg Leu 1730 1735 1740 Leu Ser Ser Thr Arg Val Pro Asn Tyr Asn Leu Ile Val Met Asp 1745 1750 1755 Glu Ala His Phe Thr Asp Pro Ser Ser Val Ala Ala Arg Gly Tyr 1760 1765 1770 Ile Ser Thr Arg Val Glu Met Gly Glu Ala Ala Ala Ile Phe Met 1775 1780 1785 Thr Ala Thr Pro Pro Gly Ala Thr Asp Pro Phe Pro Gln Ser Asn 1790 1795 1800 Ser Pro Ile Glu Asp Ile Glu Arg Glu Ile Pro Glu Arg Ser Trp 1805 1810 1815 Asn Thr Gly Phe Asp Trp Ile Thr Asp Tyr Gln Gly Lys Thr Val 1820 1825 1830 Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Asn Cys 1835 1840 1845 Leu Arg Lys Ser Gly Lys Lys Val Ile Gln Leu Ser Arg Lys Thr 1850 1855 1860 Phe Asp Thr Glu Tyr Pro Lys Thr Lys Leu Thr Asp Trp Asp Phe 1865 1870 1875 Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Arg Ala 1880 1885 1890 Gly Arg Val Ile Asp Pro Arg Arg Cys Leu Lys Pro Val Ile Leu 1895 1900 1905 Thr Asp Gly Pro Glu Arg Val Ile Leu Ala Gly Pro Ile Pro Val 1910 1915 1920 Thr Pro Ala Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg Asn 1925 1930 1935 Pro Ala Gln Glu Asp Asp Gln Tyr Val Phe Ser Gly Asp Pro Leu 1940 1945 1950 Lys Asn Asp Glu Asp His Ala His Trp Thr Glu Ala Lys Met Leu 1955 1960 1965 Leu Asp Asn Ile Tyr Thr Pro Glu Gly Ile Ile Pro Thr Leu Phe 1970 1975 1980 Gly Pro Glu Arg Glu Lys Thr Gln Ala Ile Asp Gly Glu Phe Arg 1985 1990 1995 Leu Arg Gly Glu Gln Arg Lys Thr Phe Val Glu Leu Met Arg Arg 2000 2005 2010 Gly Asp Leu Pro Val Trp Leu Ser Tyr Lys Val Ala Ser Ala Gly 2015 2020 2025 Ile Ser Tyr Lys Asp Arg Glu Trp Cys Phe Thr Gly Glu Arg Asn 2030 2035 2040 Asn Gln Ile Leu Glu Glu Asn Met Glu Val Glu Ile Trp Thr Arg 2045 2050 2055 Glu Gly Glu Lys Lys Lys Leu Arg Pro Arg Trp Leu Asp Ala Arg 2060 2065 2070 Val Tyr Ala Asp Pro Met Ala Leu Lys Asp Phe Lys Glu Phe Ala 2075 2080 2085 Ser Gly Arg Lys Ser Ile Thr Leu Asp Ile Leu Thr Glu Ile Ala 2090 2095 2100 Ser Leu Pro Thr Tyr Leu Ser Ser Arg Ala Lys Leu Ala Leu Asp 2105 2110 2115 Asn Ile Val Met Leu His Thr Thr Glu Arg Gly Gly Arg Ala Tyr 2120 2125 2130 Gln His Ala Leu Asn Glu Leu Pro Glu Ser Leu Glu Thr Leu Met 2135 2140 2145 Leu Val Ala Leu Leu Gly Ala Met Thr Ala Gly Ile Phe Leu Phe 2150 2155 2160 Phe Met Gln Gly Lys Gly Ile Gly Lys Leu Ser Met Gly Leu Ile 2165 2170 2175 Thr Ile Ala Val Ala Ser Gly Leu Phe Trp Val Ala Glu Ile Gln 2180 2185 2190 Pro Gln Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe Leu Met 2195 2200 2205 Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln Asp 2210 2215 2220 Asn Gln Leu Ile Tyr Val Ile Leu Thr Ile Leu Thr Ile Ile Gly 2225 2230 2235 Leu Ile Ala Ala Asn Glu Met Gly Leu Ile Glu Lys Thr Lys Thr 2240 2245 2250 Asp Phe Gly Phe Tyr Gln Val Lys Thr Glu Thr Thr Ile Leu Asp 2255 2260 2265 Val Asp Leu Arg Pro Ala Ser Ala Trp Thr Leu Tyr Ala Val Ala 2270 2275 2280 Thr Thr Xaa Leu Thr Pro Met Leu Arg His Thr Ile Glu Asn Thr 2285 2290 2295 Ser Ala Asn Leu Ser Leu Ala Ala Ile Ala Asn Gln Ala Ala Val 2300 2305 2310 Leu Met Gly Leu Gly Lys Gly Trp Pro Leu His Arg Met Asp Leu 2315 2320 2325 Gly Val Pro Leu Leu Ala Met Gly Cys Tyr Ser Gln Val Asn Pro 2330 2335 2340 Thr Thr Leu Thr Ala Ser Leu Val Met Leu Ser Val His Tyr Ala 2345 2350 2355 Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr Arg Glu Ala Gln 2360 2365 2370 Lys Arg Thr Ala Ala Gly Ile Met Lys Asn Pro Thr Val Asp Gly 2375 2380 2385 Ile Thr Val Ile Asp Leu Glu Pro Ile Ser Tyr Asp Pro Lys Phe 2390 2395 2400 Glu Lys Gln Leu Gly Gln Val Met Leu Leu Val Leu Cys Ala Gly 2405 2410 2415 Gln Leu Leu Leu Met Arg Thr Thr Trp Ala Phe Cys Glu Val Leu 2420 2425 2430 Thr Leu Ala Thr Gly Pro Ile Leu Thr Leu Trp Glu Gly Asn Pro 2435 2440 2445 Gly Arg Phe Trp Asn Thr Thr Ile Ala Val Ser Thr Ala Asn Ile 2450 2455 2460 Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu Ala Phe Ser Leu 2465 2470 2475 Ile Lys Asn Xaa Gln Thr Pro Arg Arg Gly Thr Gly Thr Thr Gly 2480 2485 2490 Glu Thr Leu Gly Glu Lys Trp Lys Arg Gln Leu Asn Ser Leu Xaa 2495 2500 2505 Arg Lys Glu Phe Glu Glu Tyr Lys Arg Ser Gly Ile Leu Glu Val 2510 2515 2520 Asp Arg Thr Glu Ala Lys Ser Ala Leu Lys Asp Gly Ser Lys Ile 2525 2530 2535 Lys His Ala Val Ser Arg Gly Ser Ser Lys Ile Arg Trp Ile Val 2540 2545 2550 Glu Arg Gly Met Val Lys Pro Lys Gly Lys Val Val Asp Leu Gly 2555 2560 2565 Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Met Ala Thr Leu Lys Asn 2570 2575 2580 Val Thr Glu Val Lys Gly Tyr Thr Lys Gly Gly Pro Gly His Glu 2585 2590 2595 Glu Pro Ile Pro Met Ala Thr Tyr Gly Trp Asn Leu Val Lys Leu 2600 2605 2610 His Ser Gly Val Asp Val Phe Tyr Lys Pro Thr Glu Gln Val Asp 2615 2620 2625 Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Ser Asn Pro Thr Ile 2630 2635 2640 Glu Glu Gly Arg Thr Leu Arg Val Leu Lys Met Val Glu Pro Trp 2645 2650 2655 Leu Ser Ser Lys Pro Glu Phe Cys Ile Lys Val Leu Asn Pro Tyr 2660 2665 2670 Met Pro Thr Val Ile Glu Glu Leu Glu Lys Leu Gln Arg Lys His 2675 2680 2685 Gly Gly Asn Leu Val Arg Cys Pro Leu Ser Arg Asn Ser Thr His 2690 2695 2700 Glu Met Tyr Trp Val Ser Gly Ala Ser Gly Asn Ile Val Ser Ser 2705 2710 2715 Val Asn Thr Thr Ser Lys Met Leu Leu Asn Arg Phe Thr Thr Arg 2720 2725 2730 His Arg Lys Pro Thr Tyr Glu Lys Asp Val Asp Leu Gly Ala Gly 2735 2740 2745 Thr Arg Ser Val Ser Thr Glu Thr Glu Lys Pro Asp Met Thr Ile 2750 2755 2760 Ile Gly Arg Arg Leu Gln Arg Leu Gln Glu Glu His Lys Glu Thr 2765 2770 2775 Trp His Tyr Asp Gln Glu Asn Pro Tyr Arg Thr Trp Ala Tyr His 2780 2785 2790 Gly Ser Tyr Glu Ala Pro Ser Thr Gly Ser Ala Ser Ser Met Val 2795 2800 2805 Asn Gly Val Val Lys Leu Leu Thr Lys Pro Trp Asp Val Val Pro 2810 2815 2820 Met Val Thr Gln Leu Ala Met Thr Asp Thr Thr Pro Phe Gly Gln 2825 2830 2835 Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg Thr Pro Gln Pro 2840 2845 2850 Lys Pro Gly Thr Arg Met Val Met Thr Thr Thr Ala Asn Trp Leu 2855 2860 2865 Trp Ala Leu Leu Gly Lys Lys Lys Asn Pro Arg Leu Cys Thr Arg 2870 2875 2880 Glu Glu Phe Ile Ser Lys Val Arg Ser Asn Ala Ala Ile Gly Ala 2885 2890 2895 Val Phe Gln Glu Glu Gln Gly Trp Thr Ser Ala Ser Glu Ala Val 2900 2905 2910 Asn Asp Ser Arg Phe Trp Glu Leu Val Asp Lys Glu Arg Ala Leu 2915 2920 2925 His Gln Glu Gly Lys Cys Glu Ser Cys Val Tyr Asn Met Met Gly 2930 2935 2940 Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Arg Ala Lys Gly Ser 2945 2950 2955 Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg Phe Leu Glu Phe 2960 2965 2970 Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp Phe Gly Arg Glu 2975 2980 2985 Asn Ser Trp Ser Gly Val Glu Gly Glu Gly Leu His Arg Leu Gly 2990 2995 3000 Tyr Ile Leu Glu Glu Ile Asp Lys Lys Asp Gly Asp Leu Met Tyr 3005 3010 3015 Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile Thr Glu Asp Asp 3020 3025 3030 Leu Gln Asn Glu Glu Leu Ile Thr Glu Gln Met Ala Pro His His 3035 3040 3045 Lys Ile Leu Ala Lys Ala Ile Phe Lys Leu Thr Tyr Gln Asn Lys 3050 3055 3060 Val Val Lys Val Leu Arg Pro Thr Pro Arg Gly Ala Val Met Asp 3065 3070 3075 Ile Ile Ser Arg Lys Asp Gln Arg Gly Ser Gly Gln Val Gly Thr 3080 3085 3090 Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Val Gln Leu Ile Arg 3095 3100 3105 Gln Met Glu Ala Glu Gly Val Ile Thr Gln Asp Asp Met Gln Asn 3110 3115 3120 Pro Lys Gly Leu Lys Glu Arg Val Glu Lys Trp Leu Lys Glu Cys 3125 3130 3135 Gly Val Asp Arg Leu Lys Arg Met Ala Ile Ser Gly Asp Asp Cys 3140 3145 3150 Val Val Lys Pro Leu Asp Glu Arg Phe Gly Thr Ser Leu Leu Phe 3155 3160 3165 Leu Asn Asp Met Gly Lys Val Arg Lys Asp Ile Pro Gln Trp Glu 3170 3175 3180 Pro Ser Lys Gly Trp Lys Asn Trp Gln Glu Val Pro Phe Cys Ser 3185 3190 3195 His His Phe His Lys Ile Phe Met Lys Asp Gly Arg Ser Leu Val 3200 3205 3210 Val Pro Cys Arg Asn Gln Asp Glu Leu Ile Gly Arg Ala Arg Ile 3215 3220 3225 Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala Cys Leu Gly 3230 3235 3240 Lys Ala Tyr Ala Gln Met Trp Ser Leu Met Tyr Phe His Arg Arg 3245 3250 3255 Asp Leu Arg Leu Ala Ser Met Ala Ile Cys Ser Ala Val Pro Thr 3260 3265 3270 Glu Trp Phe Pro Thr Ser Arg Thr Thr Trp Ser Ile His Ala His 3275 3280 3285 His Gln Trp Met Thr Thr Glu Asp Met Leu Lys Val Trp Asn Arg 3290 3295 3300 Val Trp Ile Glu Asp Asn Pro Asn Met Thr Asp Lys Thr Pro Val 3305 3310 3315 His Ser Trp Glu Asp Ile Pro Tyr Leu Gly Lys Arg Glu Asp Leu 3320 3325 3330 Trp Cys Gly Ser Leu Ile Gly Leu Ser Ser Arg Ala Thr Trp Ala 3335 3340 3345 Lys Asn Ile His Thr Ala Ile Thr Gln Val Arg Asn Leu Ile Gly 3350 3355 3360 Lys Glu Glu Tyr Val Asp Tyr Met Pro Val Met Lys Arg Tyr Ser 3365 3370 3375 Ala Pro Ser Glu Ser Glu Gly Val Leu 3380 3385 <210> 9 <211> 3392 <212> PRT <213> Dengue virus type 1 <220> <221> VARIANT <222> (482)..(482) <223> Xaa stands for Lys or Glu <220> <221> VARIANT <222> (484)..(484) <223> Xaa stands for Lys or Arg <220> <221> VARIANT <222> (2357)..(2357) <223> Xaa stands for Phe or Leu <220> <221> VARIANT <222> (2364)..(2364) <223> Xaa stands for Thr or Ala <400> 9 Met Asn Asn Gln Arg Lys Lys Thr Ala Arg Pro Ser Phe Asn Met Leu 1 5 10 15 Lys Arg Ala Arg Asn Arg Val Ser Thr Val Ser Gln Leu Ala Lys Arg 20 25 30 Phe Ser Lys Gly Leu Leu Ser Gly Gln Gly Pro Met Lys Leu Val Met 35 40 45 Ala Phe Ile Ala Phe Leu Arg Phe Leu Ala Ile Pro Pro Thr Ala Gly 50 55 60 Ile Leu Ala Arg Trp Gly Ser Phe Lys Lys Asn Gly Ala Ile Lys Val 65 70 75 80 Leu Arg Gly Phe Lys Lys Glu Ile Ser Asn Met Leu Asn Ile Met Asn 85 90 95 Arg Arg Lys Arg Ser Val Thr Met Leu Leu Met Leu Met Pro Thr Ala 100 105 110 Leu Ala Phe His Leu Thr Thr Arg Gly Gly Glu Pro His Met Ile Val 115 120 125 Ser Lys Gln Glu Arg Gly Lys Ser Leu Leu Phe Lys Thr Ser Ala Gly 130 135 140 Val Asn Met Cys Thr Leu Ile Ala Met Asp Leu Gly Glu Leu Cys Glu 145 150 155 160 Asp Thr Met Thr Tyr Lys Cys Pro Arg Ile Thr Glu Ala Glu Pro Asp 165 170 175 Asp Val Asp Cys Trp Cys Asn Ala Thr Asp Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Ser Gln Thr Gly Glu His Arg Arg Asp Lys Arg Ser Val Ala 195 200 205 Leu Ala Pro His Val Gly Leu Gly Leu Glu Thr Arg Thr Glu Thr Trp 210 215 220 Met Ser Ser Glu Gly Ala Trp Lys Gln Ile Gln Arg Val Glu Thr Trp 225 230 235 240 Ala Leu Arg His Pro Gly Phe Thr Val Ile Ala Leu Phe Leu Ala His 245 250 255 Ala Ile Gly Thr Ser Ile Thr Gln Lys Gly Ile Ile Phe Ile Leu Leu 260 265 270 Met Leu Val Thr Pro Ser Met Ala Met Arg Cys Val Gly Ile Gly Ser 275 280 285 Arg Asp Phe Val Glu Gly Leu Ser Gly Ala Thr Trp Val Asp Val Val 290 295 300 Leu Glu His Gly Ser Cys Val Thr Thr Met Ala Lys Asp Lys Pro Thr 305 310 315 320 Leu Asp Ile Glu Leu Leu Lys Thr Glu Val Thr Asn Pro Ala Val Leu 325 330 335 Arg Lys Leu Cys Ile Glu Ala Lys Ile Ser Asn Thr Thr Thr Asp Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Ala Thr Leu Val Glu Glu Gln Asp Ala 355 360 365 Asn Phe Val Cys Arg Arg Thr Phe Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Ser Leu Leu Thr Cys Ala Lys Phe Lys 385 390 395 400 Cys Val Thr Lys Leu Glu Gly Lys Ile Val Gln Tyr Glu Asn Leu Lys 405 410 415 Tyr Ser Val Ile Val Thr Val His Thr Gly Asp Gln His Gln Val Gly 420 425 430 Asn Glu Ser Thr Glu His Gly Thr Thr Ala Thr Ile Thr Pro Gln Ala 435 440 445 Pro Thr Thr Glu Ile Gln Leu Thr Asp Tyr Gly Ala Leu Thr Leu Asp 450 455 460 Cys Ser Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Val Leu Leu Thr 465 470 475 480 Met Xaa Lys Xaa Ser Trp Leu Val His Lys Gln Trp Phe Leu Asp Leu 485 490 495 Pro Leu Pro Trp Thr Ser Gly Ala Ser Thr Ser Gln Glu Thr Trp Asn 500 505 510 Arg Gln Asp Leu Leu Val Thr Phe Lys Thr Ala His Ala Lys Lys Gln 515 520 525 Glu Val Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu 530 535 540 Thr Gly Ala Thr Glu Ile Gln Thr Ser Gly Thr Thr Thr Ile Phe Ala 545 550 555 560 Gly His Leu Lys Cys Arg Leu Arg Met Asp Lys Leu Thr Leu Lys Gly 565 570 575 Met Ser Tyr Val Met Cys Thr Gly Ser Phe Lys Leu Glu Lys Glu Val 580 585 590 Ala Glu Thr Gln His Gly Thr Val Leu Val Gln Ile Lys Tyr Glu Gly 595 600 605 Thr Asp Ala Pro Cys Lys Ile Pro Phe Ser Thr Gln Asp Glu Lys Gly 610 615 620 Val Thr Gln Asn Gly Arg Leu Ile Thr Ala Asn Pro Ile Val Thr Asp 625 630 635 640 Lys Glu Lys Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser 645 650 655 Tyr Ile Val Ile Gly Ala Gly Glu Lys Ala Leu Lys Leu Ser Trp Phe 660 665 670 Lys Lys Gly Ser Ser Ile Gly Lys Met Phe Glu Ala Thr Ala Arg Gly 675 680 685 Ala Arg Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 690 695 700 Ile Gly Gly Val Phe Thr Ser Val Gly Lys Leu Val His Gln Ile Phe 705 710 715 720 Gly Thr Ala Tyr Gly Val Leu Phe Ser Gly Val Ser Trp Thr Met Lys 725 730 735 Ile Gly Ile Gly Val Leu Leu Thr Trp Leu Gly Leu Asn Ser Arg Asn 740 745 750 Thr Ser Leu Ser Met Thr Cys Ile Ala Val Gly Leu Val Thr Leu Tyr 755 760 765 Leu Gly Val Met Val Gln Ala Asp Ser Gly Cys Val Ile Asn Trp Lys 770 775 780 Gly Arg Glu Leu Lys Cys Gly Ser Gly Ile Phe Val Thr Asn Glu Val 785 790 795 800 His Thr Trp Thr Glu Gln Tyr Lys Phe Gln Ala Asp Ser Pro Lys Arg 805 810 815 Leu Ser Ala Ala Ile Gly Lys Ala Trp Glu Glu Gly Val Cys Gly Ile 820 825 830 Arg Ser Ala Thr Arg Leu Glu Asn Ile Met Trp Lys Gln Ile Ser Asn 835 840 845 Glu Leu Asn His Ile Leu Leu Glu Asn Asp Met Lys Phe Thr Val Val 850 855 860 Val Gly Asp Val Ala Gly Ile Leu Ala Gln Gly Lys Lys Met Ile Arg 865 870 875 880 Pro Gln Pro Met Glu His Lys Tyr Ser Trp Lys Ser Trp Gly Lys Ala 885 890 895 Lys Ile Ile Gly Ala Asp Val Gln Asn Ser Thr Phe Ile Ile Asp Gly 900 905 910 Pro Asn Thr Pro Glu Cys Pro Asp Asp Gln Arg Ala Trp Asn Ile Trp 915 920 925 Glu Val Glu Asp Tyr Gly Phe Gly Ile Phe Thr Thr Asn Ile Trp Leu 930 935 940 Lys Leu Arg Asp Ser Tyr Thr Gln Leu Cys Asp His Arg Leu Met Ser 945 950 955 960 Ala Ala Ile Lys Asp Ser Lys Ala Val His Ala Asp Met Gly Tyr Trp 965 970 975 Ile Glu Ser Glu Lys Asn Glu Thr Trp Lys Leu Ala Arg Ala Ser Phe 980 985 990 Ile Glu Val Lys Thr Cys Ile Trp Pro Lys Ser His Thr Leu Trp Ser 995 1000 1005 Asn Gly Val Leu Glu Ser Glu Met Ile Ile Pro Lys Ile Tyr Gly 1010 1015 1020 Gly Pro Ile Ser Gln His Asn Tyr Arg Pro Gly Tyr Phe Thr Gln 1025 1030 1035 Thr Val Gly Pro Trp His Leu Gly Lys Leu Glu Leu Asp Phe Asp 1040 1045 1050 Leu Cys Glu Gly Thr Thr Val Val Val Asp Glu His Cys Gly Asn 1055 1060 1065 Arg Gly Pro Ser Leu Arg Thr Thr Thr Val Thr Gly Lys Ile Ile 1070 1075 1080 His Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Phe 1085 1090 1095 Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Val 1100 1105 1110 Lys Glu Lys Glu Glu Asn Leu Val Lys Ser Met Val Ser Ala Gly 1115 1120 1125 Ser Gly Glu Val Asp Ser Phe Ser Leu Gly Leu Leu Cys Ile Ser 1130 1135 1140 Ile Met Ile Glu Glu Val Met Arg Ser Arg Trp Ser Arg Lys Met 1145 1150 1155 Leu Met Thr Gly Thr Leu Ala Val Phe Leu Leu Leu Ile Met Gly 1160 1165 1170 Gln Leu Thr Trp Asn Asp Leu Ile Arg Leu Cys Ile Met Val Gly 1175 1180 1185 Ala Asn Ala Ser Asp Arg Met Gly Met Gly Thr Thr Tyr Leu Ala 1190 1195 1200 Leu Met Ala Thr Phe Lys Met Arg Pro Met Phe Ala Val Gly Leu 1205 1210 1215 Leu Phe Arg Arg Leu Thr Ser Arg Glu Val Leu Leu Leu Thr Ile 1220 1225 1230 Gly Leu Ser Leu Val Ala Ser Val Glu Leu Pro Asn Ser Leu Glu 1235 1240 1245 Glu Leu Gly Asp Gly Leu Ala Met Gly Ile Met Ile Leu Lys Leu 1250 1255 1260 Leu Thr Asp Phe Gln Ser His Gln Leu Trp Ala Thr Leu Leu Ser 1265 1270 1275 Leu Thr Phe Ile Lys Thr Thr Phe Ser Leu His Tyr Ala Trp Lys 1280 1285 1290 Thr Met Ala Met Val Leu Ser Ile Val Ser Leu Phe Pro Leu Cys 1295 1300 1305 Leu Ser Thr Thr Ser Gln Lys Thr Thr Trp Leu Pro Val Leu Leu 1310 1315 1320 Gly Ser Leu Gly Cys Lys Pro Leu Thr Met Phe Leu Ile Ala Glu 1325 1330 1335 Asn Lys Ile Trp Gly Arg Lys Ser Trp Pro Leu Asn Glu Gly Ile 1340 1345 1350 Met Ala Val Gly Ile Val Ser Ile Leu Leu Ser Ser Leu Leu Lys 1355 1360 1365 Asn Asp Val Pro Leu Ala Gly Pro Leu Ile Ala Gly Gly Met Leu 1370 1375 1380 Ile Ala Cys Tyr Val Ile Ser Gly Ser Ser Ala Asp Leu Ser Leu 1385 1390 1395 Glu Lys Ala Ala Glu Val Ser Trp Glu Glu Glu Ala Glu His Ser 1400 1405 1410 Gly Ala Ser His Asn Ile Leu Val Glu Val Gln Asp Asp Gly Thr 1415 1420 1425 Met Lys Ile Lys Asp Glu Glu Arg Asp Asp Thr Leu Thr Ile Leu 1430 1435 1440 Leu Lys Ala Thr Leu Leu Ala Val Ser Gly Val Tyr Pro Leu Ser 1445 1450 1455 Ile Pro Ala Thr Leu Phe Val Trp Tyr Phe Trp Gln Lys Lys Lys 1460 1465 1470 Gln Arg Ser Gly Val Leu Trp Asp Thr Pro Ser Pro Pro Glu Val 1475 1480 1485 Glu Arg Ala Val Leu Asp Asp Gly Ile Tyr Arg Ile Met Gln Arg 1490 1495 1500 Gly Leu Leu Gly Arg Ser Gln Val Gly Val Gly Val Phe Gln Glu 1505 1510 1515 Asn Val Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu 1520 1525 1530 Met Tyr Gln Gly Lys Arg Leu Glu Pro Ser Trp Ala Ser Val Lys 1535 1540 1545 Lys Asp Leu Ile Ser Tyr Gly Gly Gly Trp Arg Leu Gln Gly Ser 1550 1555 1560 Trp Asn Thr Gly Glu Glu Val Gln Val Ile Ala Val Glu Pro Gly 1565 1570 1575 Lys Asn Pro Lys Asn Val Gln Thr Ala Pro Gly Thr Phe Lys Thr 1580 1585 1590 Pro Glu Gly Glu Val Gly Ala Ile Ala Leu Asp Phe Lys Pro Gly 1595 1600 1605 Thr Ser Gly Ser Pro Ile Val Asn Arg Glu Gly Lys Ile Val Gly 1610 1615 1620 Leu Tyr Gly Asn Gly Val Val Thr Thr Ser Gly Thr Tyr Val Ser 1625 1630 1635 Ala Ile Ala Gln Ala Lys Ala Ser Gln Glu Gly Pro Leu Pro Glu 1640 1645 1650 Ile Glu Asp Glu Val Phe Arg Lys Arg Lys Leu Thr Ile Met Asp 1655 1660 1665 Leu His Pro Gly Ser Gly Lys Thr Arg Arg Tyr Leu Pro Ala Ile 1670 1675 1680 Val Arg Glu Ala Ile Lys Arg Lys Leu Arg Thr Leu Ile Leu Ala 1685 1690 1695 Pro Thr Arg Val Val Ala Ser Glu Met Ala Glu Ala Leu Lys Gly 1700 1705 1710 Met Pro Ile Arg Tyr Gln Thr Thr Ala Val Lys Ser Glu His Thr 1715 1720 1725 Gly Lys Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met 1730 1735 1740 Arg Leu Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Met Ile Ile 1745 1750 1755 Met Asp Glu Ala His Phe Thr Asp Pro Ser Ser Ile Ala Ala Arg 1760 1765 1770 Gly Tyr Ile Ser Thr Arg Val Gly Met Gly Glu Ala Ala Ala Ile 1775 1780 1785 Phe Met Thr Ala Thr Pro Pro Gly Ser Val Glu Ala Phe Pro Gln 1790 1795 1800 Ser Asn Ala Val Ile Gln Asp Glu Glu Arg Asp Ile Pro Glu Arg 1805 1810 1815 Ser Trp Asn Ser Gly Tyr Glu Trp Ile Thr Asp Phe Pro Gly Lys 1820 1825 1830 Thr Val Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala 1835 1840 1845 Asn Cys Leu Arg Lys Asn Gly Lys Arg Val Ile Gln Leu Ser Arg 1850 1855 1860 Lys Thr Phe Asp Thr Glu Tyr Gln Lys Thr Lys Asn Asn Asp Trp 1865 1870 1875 Asp Tyr Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe 1880 1885 1890 Arg Ala Asp Arg Val Ile Asp Pro Arg Arg Cys Leu Lys Pro Val 1895 1900 1905 Ile Leu Lys Asp Gly Pro Glu Arg Val Ile Leu Ala Gly Pro Met 1910 1915 1920 Pro Val Thr Val Ala Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly 1925 1930 1935 Arg Asn His Asn Lys Glu Gly Asp Gln Tyr Ile Tyr Met Gly Gln 1940 1945 1950 Pro Leu Asn Asn Asp Glu Asp His Ala His Trp Thr Glu Ala Lys 1955 1960 1965 Met Leu Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ala 1970 1975 1980 Leu Phe Glu Pro Glu Arg Glu Lys Ser Ala Ala Ile Asp Gly Glu 1985 1990 1995 Tyr Arg Leu Arg Gly Glu Ala Arg Lys Thr Phe Val Glu Leu Met 2000 2005 2010 Arg Arg Gly Asp Leu Pro Val Trp Leu Ser Tyr Lys Val Ala Ser 2015 2020 2025 Glu Gly Phe Gln Tyr Ser Asp Arg Arg Trp Cys Phe Asp Gly Glu 2030 2035 2040 Arg Asn Asn Gln Val Leu Glu Glu Asn Met Asp Val Glu Ile Trp 2045 2050 2055 Thr Lys Glu Gly Glu Arg Lys Lys Leu Arg Pro Arg Trp Leu Asp 2060 2065 2070 Ala Arg Thr Tyr Ser Asp Pro Leu Ala Leu Arg Glu Phe Lys Glu 2075 2080 2085 Phe Ala Ala Gly Arg Arg Ser Val Ser Gly Asp Leu Ile Leu Glu 2090 2095 2100 Ile Gly Lys Leu Pro Gln His Leu Thr Gln Arg Ala Gln Asn Ala 2105 2110 2115 Leu Asp Asn Leu Val Met Leu His Asn Ser Glu Gln Gly Gly Arg 2120 2125 2130 Ala Tyr Arg His Ala Met Glu Glu Leu Pro Asp Thr Ile Glu Thr 2135 2140 2145 Leu Met Leu Leu Ala Leu Ile Ala Val Leu Thr Gly Gly Val Thr 2150 2155 2160 Leu Phe Phe Leu Ser Gly Lys Gly Leu Gly Lys Thr Ser Ile Gly 2165 2170 2175 Leu Leu Cys Val Met Ala Ser Ser Val Leu Leu Trp Met Ala Ser 2180 2185 2190 Val Glu Pro His Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe 2195 2200 2205 Leu Met Val Leu Leu Ile Pro Glu Pro Asp Arg Gln Arg Thr Pro 2210 2215 2220 Gln Asp Asn Gln Leu Ala Tyr Val Val Ile Gly Leu Leu Phe Met 2225 2230 2235 Ile Leu Thr Val Ala Ala Asn Glu Met Gly Leu Leu Glu Thr Thr 2240 2245 2250 Lys Lys Asp Leu Gly Ile Gly His Val Ala Val Glu Asn His His 2255 2260 2265 His Ala Ala Met Leu Asp Val Asp Leu His Pro Ala Ser Ala Trp 2270 2275 2280 Thr Leu Tyr Ala Val Ala Thr Thr Ile Ile Thr Pro Met Met Arg 2285 2290 2295 His Thr Ile Glu Asn Thr Thr Ala Asn Ile Ser Leu Thr Ala Ile 2300 2305 2310 Ala Asn Gln Ala Ala Ile Leu Met Gly Leu Asp Lys Gly Trp Pro 2315 2320 2325 Ile Ser Lys Met Asp Ile Gly Val Pro Leu Leu Ala Leu Gly Cys 2330 2335 2340 Tyr Ser Gln Val Asn Pro Leu Thr Leu Thr Ala Ala Val Xaa Met 2345 2350 2355 Leu Val Ala His Tyr Xaa Ile Ile Gly Pro Gly Leu Gln Ala Lys 2360 2365 2370 Ala Thr Arg Glu Ala Gln Lys Arg Thr Ala Ala Gly Ile Met Lys 2375 2380 2385 Asn Pro Thr Ile Asp Gly Ile Val Ala Ile Asp Leu Asp Pro Val 2390 2395 2400 Val Tyr Asp Ala Lys Phe Glu Lys Gln Leu Gly Gln Ile Met Leu 2405 2410 2415 Leu Ile Leu Cys Thr Ser Gln Ile Leu Leu Met Arg Thr Thr Trp 2420 2425 2430 Ala Leu Cys Glu Ser Ile Thr Leu Ala Thr Gly Pro Leu Thr Thr 2435 2440 2445 Leu Trp Glu Gly Ser Pro Gly Lys Phe Trp Asn Thr Thr Ile Ala 2450 2455 2460 Val Ser Met Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala 2465 2470 2475 Gly Leu Ala Phe Ser Leu Met Lys Ser Leu Gly Gly Gly Arg Arg 2480 2485 2490 Gly Thr Gly Ala Gln Gly Glu Thr Leu Gly Glu Lys Trp Lys Arg 2495 2500 2505 Gln Leu Asn Gln Leu Ser Lys Ser Glu Phe Asn Thr Tyr Lys Arg 2510 2515 2520 Ser Gly Ile Met Glu Val Asp Arg Ser Glu Ala Lys Glu Gly Leu 2525 2530 2535 Lys Arg Gly Glu Thr Thr Lys His Ala Val Ser Arg Gly Thr Ala 2540 2545 2550 Lys Leu Arg Trp Phe Val Glu Arg Asn Leu Val Lys Pro Glu Gly 2555 2560 2565 Lys Val Ile Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr 2570 2575 2580 Cys Ala Gly Leu Lys Lys Val Thr Glu Val Lys Gly Tyr Thr Lys 2585 2590 2595 Gly Gly Pro Gly His Glu Glu Pro Ile Pro Met Ala Thr Tyr Gly 2600 2605 2610 Trp Asn Leu Val Lys Leu His Ser Gly Lys Asp Val Phe Phe Ile 2615 2620 2625 Pro Pro Glu Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser 2630 2635 2640 Ser Pro Asn Pro Thr Ile Glu Glu Gly Arg Thr Leu Arg Val Leu 2645 2650 2655 Lys Met Val Glu Pro Trp Leu Arg Gly Asn Gln Phe Cys Ile Lys 2660 2665 2670 Ile Leu Asn Pro Tyr Met Pro Ser Val Val Glu Thr Leu Glu Gln 2675 2680 2685 Met Gln Arg Lys His Gly Gly Met Leu Val Arg Asn Pro Leu Ser 2690 2695 2700 Arg Asn Ser Thr His Glu Met Tyr Trp Val Ser Cys Gly Thr Gly 2705 2710 2715 Asn Ile Val Ser Ala Val Asn Met Thr Ser Arg Met Leu Leu Asn 2720 2725 2730 Arg Phe Thr Met Ala His Arg Lys Pro Thr Tyr Glu Arg Asp Val 2735 2740 2745 Asp Leu Gly Ala Gly Thr Arg His Val Ala Val Glu Pro Glu Val 2750 2755 2760 Ala Asn Leu Asp Ile Ile Gly Gln Arg Ile Glu Asn Ile Lys His 2765 2770 2775 Glu His Lys Ser Thr Trp His Tyr Asp Glu Asp Asn Pro Tyr Lys 2780 2785 2790 Thr Trp Ala Tyr His Gly Ser Tyr Glu Val Lys Pro Ser Gly Ser 2795 2800 2805 Ala Ser Ser Met Val Asn Gly Val Val Lys Leu Leu Thr Lys Pro 2810 2815 2820 Trp Asp Val Ile Pro Met Val Thr Gln Ile Ala Met Thr Asp Thr 2825 2830 2835 Thr Pro Phe Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr 2840 2845 2850 Arg Thr Pro Lys Ala Lys Arg Gly Thr Ala Gln Ile Met Glu Val 2855 2860 2865 Thr Ala Arg Trp Leu Trp Gly Phe Leu Ser Arg Asn Lys Lys Pro 2870 2875 2880 Arg Ile Cys Thr Arg Glu Glu Phe Thr Arg Lys Val Arg Ser Asn 2885 2890 2895 Ala Ala Ile Gly Ala Val Phe Val Asp Glu Asn Gln Trp Asn Ser 2900 2905 2910 Ala Lys Glu Ala Val Glu Asp Asp Arg Phe Trp Asp Leu Val His 2915 2920 2925 Arg Glu Arg Glu Leu His Lys Gln Gly Lys Cys Ala Thr Cys Val 2930 2935 2940 Tyr Asn Met Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly 2945 2950 2955 Lys Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala 2960 2965 2970 Arg Phe Leu Glu Phe Glu Ala Leu Gly Phe Met Asn Glu Asp His 2975 2980 2985 Trp Phe Ser Arg Glu Asn Ser Leu Ser Gly Val Glu Gly Glu Gly 2990 2995 3000 Leu His Lys Leu Gly Tyr Ile Leu Arg Asp Ile Ser Lys Ile Pro 3005 3010 3015 Gly Gly Asn Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg 3020 3025 3030 Ile Thr Glu Asp Asp Leu Gln Asn Glu Ala Lys Ile Thr Asp Ile 3035 3040 3045 Met Glu Pro Glu His Ala Leu Leu Ala Lys Ser Ile Phe Lys Leu 3050 3055 3060 Thr Tyr Gln Asn Lys Val Val Arg Val Gln Arg Pro Ala Lys Asn 3065 3070 3075 Gly Thr Val Met Asp Val Ile Ser Arg Arg Asp Gln Arg Gly Ser 3080 3085 3090 Gly Gln Val Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu 3095 3100 3105 Ala Gln Leu Ile Arg Gln Met Glu Ser Glu Gly Ile Phe Ser Pro 3110 3115 3120 Asn Glu Leu Glu Thr Pro Asn Leu Ala Glu Arg Val Leu Asp Trp 3125 3130 3135 Leu Glu Lys Tyr Gly Val Glu Arg Leu Lys Arg Met Ala Ile Ser 3140 3145 3150 Gly Asp Asp Cys Val Val Lys Pro Ile Asp Asp Arg Phe Ala Thr 3155 3160 3165 Ala Leu Thr Ala Leu Asn Asp Met Gly Lys Val Arg Lys Asp Ile 3170 3175 3180 Pro Gln Trp Glu Pro Ser Lys Gly Trp Asn Asp Trp Gln Gln Val 3185 3190 3195 Pro Phe Cys Ser His His Phe His Gln Leu Ile Met Lys Asp Gly 3200 3205 3210 Arg Glu Ile Val Val Pro Cys Arg Asn Gln Asp Glu Leu Val Gly 3215 3220 3225 Arg Ala Arg Val Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr 3230 3235 3240 Ala Cys Leu Gly Lys Ser Tyr Ala Gln Met Trp Gln Leu Met Tyr 3245 3250 3255 Phe His Arg Arg Asp Leu Arg Leu Ala Ala Asn Ala Ile Cys Ser 3260 3265 3270 Ala Val Pro Val Asp Trp Val Pro Thr Ser Arg Thr Thr Trp Ser 3275 3280 3285 Ile His Ala His His Gln Trp Met Thr Thr Glu Asp Met Leu Ser 3290 3295 3300 Val Trp Asn Arg Val Trp Ile Glu Glu Asn Pro Trp Met Glu Asp 3305 3310 3315 Lys Thr Arg Ile Ser Ser Trp Glu Asp Val Pro Tyr Leu Gly Lys 3320 3325 3330 Arg Glu Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ala Arg 3335 3340 3345 Ala Thr Trp Ala Thr Asn Ile Gln Val Ala Ile Asn Gln Val Arg 3350 3355 3360 Arg Leu Ile Gly Asn Glu Asn Tyr Ser Asp Tyr Met Thr Ser Met 3365 3370 3375 Lys Arg Phe Lys Asn Glu Ser Glu Pro Glu Gly Ala Leu Trp 3380 3385 3390 <210> 10 <211> 3391 <212> PRT <213> Dengue virus type 2 <220> <221> VARIANT <222> (1461)..(1461) <223> Xaa stands for Val or Ala <220> <221> VARIANT <222> (2190)..(2190) <223> Xaa stands for Tyr or Cys <220> <221> VARIANT <222> (2347)..(2347) <223> Xaa stands for Leu or Pro <220> <221> VARIANT <222> (2828)..(2828) <223> Xaa stands for Thr or Met <220> <221> VARIANT <222> (3020)..(3020) <223> Xaa stands for Thr or Ala <400> 10 Met Asn Asn Gln Arg Lys Lys Ala Lys Asn Thr Pro Phe Asn Met Leu 1 5 10 15 Lys Arg Glu Arg Asn Arg Val Ser Thr Val Gln Gln Leu Thr Lys Arg 20 25 30 Phe Ser Leu Gly Met Leu Gln Gly Arg Gly Pro Leu Lys Leu Phe Met 35 40 45 Ala Leu Val Ala Phe Leu Arg Phe Leu Thr Ile Pro Pro Thr Ala Gly 50 55 60 Ile Leu Lys Arg Trp Gly Thr Ile Lys Lys Ser Lys Ala Ile Asn Val 65 70 75 80 Leu Arg Gly Phe Arg Lys Glu Ile Gly Arg Met Leu Asn Ile Leu Asn 85 90 95 Arg Arg Arg Arg Ser Ala Gly Met Ile Ile Met Leu Ile Pro Thr Val 100 105 110 Met Ala Phe His Leu Thr Thr Arg Asn Gly Glu Pro His Met Ile Val 115 120 125 Gly Ile Gln Glu Lys Gly Lys Ser Leu Leu Phe Lys Thr Glu Asn Gly 130 135 140 Val Asn Met Cys Thr Leu Met Ala Met Asp Leu Gly Glu Leu Cys Glu 145 150 155 160 Asp Thr Ile Thr Tyr Lys Cys Pro Leu Leu Arg Gln Asn Glu Pro Glu 165 170 175 Asp Ile Asp Cys Trp Cys Asn Ser Thr Ser Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Thr Thr Thr Gly Glu His Arg Arg Glu Lys Arg Ser Val Ala 195 200 205 Leu Val Pro His Val Gly Met Gly Leu Glu Thr Arg Thr Glu Thr Trp 210 215 220 Met Ser Ser Glu Gly Ala Trp Lys His Ala Gln Arg Ile Glu Thr Trp 225 230 235 240 Ile Leu Arg His Pro Gly Phe Thr Ile Met Ala Ala Ile Leu Ala Tyr 245 250 255 Thr Ile Gly Thr Thr His Phe Gln Arg Val Leu Ile Phe Ile Leu Leu 260 265 270 Thr Ala Val Ala Pro Ser Met Thr Met Arg Cys Ile Gly Ile Ser Asn 275 280 285 Arg Asp Phe Val Glu Gly Val Ser Gly Gly Ser Trp Val Asp Ile Val 290 295 300 Leu Glu His Gly Ser Cys Val Thr Thr Met Ala Lys Asn Lys Pro Thr 305 310 315 320 Leu Asp Phe Glu Leu Ile Lys Thr Glu Ala Lys Gln Pro Ala Thr Leu 325 330 335 Arg Lys Tyr Cys Ile Glu Ala Lys Leu Thr Asn Thr Thr Thr Glu Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Pro Ser Leu Lys Glu Glu Gln Asp Lys 355 360 365 Arg Phe Val Cys Lys His Ser Met Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Gly Ile Val Thr Cys Ala Met Phe Lys 385 390 395 400 Cys Lys Lys Asn Met Glu Gly Lys Ile Val Gln Pro Glu Asn Leu Glu 405 410 415 Tyr Thr Ile Val Val Thr Pro His Ser Gly Glu Glu His Ala Val Gly 420 425 430 Asn Asp Thr Gly Lys His Gly Lys Glu Ile Lys Val Thr Pro Gln Ser 435 440 445 Ser Ile Thr Glu Ala Glu Leu Thr Gly Tyr Gly Thr Val Thr Met Glu 450 455 460 Cys Ser Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Val Leu Leu Gln 465 470 475 480 Met Glu Asn Lys Ala Trp Leu Val His Arg Gln Trp Phe Leu Asp Leu 485 490 495 Pro Leu Pro Trp Leu Pro Gly Ala Asp Lys Gln Glu Ser Asn Trp Ile 500 505 510 Gln Lys Glu Thr Leu Val Thr Phe Lys Asn Pro His Ala Lys Lys Gln 515 520 525 Asp Val Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu 530 535 540 Thr Gly Ala Thr Glu Ile Gln Met Ser Ser Gly Asn Leu Leu Phe Thr 545 550 555 560 Gly His Leu Lys Cys Arg Leu Arg Met Asp Lys Leu Gln Leu Lys Gly 565 570 575 Met Ser Tyr Ser Met Cys Thr Gly Lys Phe Lys Val Val Lys Glu Ile 580 585 590 Ala Glu Thr Gln His Gly Thr Ile Val Ile Arg Val Gln Tyr Glu Gly 595 600 605 Asp Gly Ser Pro Cys Lys Ile Pro Phe Glu Ile Met Asp Leu Glu Lys 610 615 620 Arg Tyr Val Leu Gly Arg Leu Ile Thr Val Asn Pro Ile Val Thr Glu 625 630 635 640 Lys Asp Ser Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Asp Ser 645 650 655 Tyr Ile Ile Ile Gly Val Glu Pro Gly Gln Leu Lys Leu Asn Trp Phe 660 665 670 Lys Lys Gly Ser Ser Ile Gly Gln Met Phe Glu Thr Thr Met Arg Gly 675 680 685 Ala Lys Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 690 695 700 Leu Gly Gly Val Phe Thr Ser Ile Gly Lys Ala Leu His Gln Val Phe 705 710 715 720 Gly Ala Ile Tyr Gly Ala Ala Phe Ser Gly Val Ser Trp Thr Met Lys 725 730 735 Ile Leu Ile Gly Val Ile Ile Thr Trp Ile Gly Met Asn Ser Arg Ser 740 745 750 Thr Ser Leu Ser Val Ser Leu Val Leu Val Gly Ile Val Thr Leu Tyr 755 760 765 Leu Gly Val Met Val Gln Ala Asp Ser Gly Cys Val Val Ser Trp Lys 770 775 780 Asn Lys Glu Leu Lys Cys Gly Ser Gly Ile Phe Ile Thr Asp Asn Val 785 790 795 800 His Thr Trp Thr Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ser Lys 805 810 815 Leu Ala Ser Ala Ile Gln Lys Ala Gln Glu Glu Gly Ile Cys Gly Ile 820 825 830 Arg Ser Val Thr Arg Leu Glu Asn Leu Met Trp Lys Gln Ile Thr Pro 835 840 845 Glu Leu Asn His Ile Leu Ala Glu Asn Glu Val Lys Leu Thr Ile Met 850 855 860 Thr Gly Asp Ile Lys Gly Ile Met Gln Ala Gly Lys Arg Ser Leu Arg 865 870 875 880 Pro Gln Pro Thr Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala 885 890 895 Lys Met Leu Ser Thr Glu Ser Tyr Asn Gln Thr Phe Leu Ile Asp Gly 900 905 910 Pro Glu Thr Ala Glu Cys Pro Asn Thr Asn Arg Ala Trp Asn Ser Leu 915 920 925 Glu Val Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu 930 935 940 Lys Leu Lys Glu Lys Gln Asp Ala Phe Cys Asp Ser Lys Leu Met Ser 945 950 955 960 Ala Ala Ile Lys Asp Asn Arg Ala Val His Ala Asp Met Gly Tyr Trp 965 970 975 Ile Glu Ser Ala Leu Asn Asp Thr Trp Lys Ile Glu Lys Ala Ser Phe 980 985 990 Ile Glu Val Lys Asn Cys His Trp Pro Lys Ser His Thr Leu Trp Ser 995 1000 1005 Asn Gly Val Leu Glu Ser Glu Met Ile Ile Pro Lys Asn Leu Ala 1010 1015 1020 Gly Pro Val Ser Gln His Asn Tyr Arg Pro Gly Tyr His Thr Gln 1025 1030 1035 Val Ala Gly Pro Trp His Leu Gly Lys Leu Glu Met Asp Phe Asp 1040 1045 1050 Phe Cys Asp Gly Thr Thr Val Ile Val Thr Glu Asp Cys Gly Asn 1055 1060 1065 Arg Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys Leu Ile 1070 1075 1080 Thr Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Tyr 1085 1090 1095 Arg Gly Glu Asn Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu 1100 1105 1110 Lys Glu Lys Glu Glu Asn Leu Val Asn Ser Leu Val Thr Ala Gly 1115 1120 1125 His Gly Gln Val Asp Asn Phe Ser Leu Gly Val Leu Gly Met Ala 1130 1135 1140 Leu Phe Leu Glu Glu Met Leu Arg Thr Arg Val Gly Thr Lys His 1145 1150 1155 Ala Ile Leu Leu Val Ala Val Ser Phe Val Thr Leu Ile Thr Gly 1160 1165 1170 Asn Met Ser Phe Lys Asp Leu Gly Arg Val Val Val Met Val Gly 1175 1180 1185 Ala Thr Met Ala Asp Asp Ile Gly Met Gly Val Thr Tyr Leu Ala 1190 1195 1200 Leu Leu Ala Ala Phe Lys Val Arg Pro Thr Phe Ala Ala Gly Leu 1205 1210 1215 Leu Leu Arg Lys Leu Thr Ser Lys Glu Leu Met Met Thr Thr Ile 1220 1225 1230 Gly Ile Val Leu Leu Ser Gln Ser Thr Ile Pro Glu Thr Val Leu 1235 1240 1245 Glu Leu Thr Asp Ala Leu Ala Leu Gly Met Met Val Leu Lys Ile 1250 1255 1260 Val Arg Asn Met Glu Lys Tyr Gln Leu Ala Val Thr Ile Met Ala 1265 1270 1275 Ile Leu Cys Val Pro Asn Ala Val Ile Leu Gln Asn Ala Trp Lys 1280 1285 1290 Val Ser Cys Thr Ile Leu Ala Val Val Ser Val Ser Pro Leu Phe 1295 1300 1305 Leu Thr Ser Ser Gln Gln Lys Thr Asp Trp Ile Pro Leu Ala Leu 1310 1315 1320 Thr Ile Lys Gly Leu Asn Pro Thr Ala Ile Phe Leu Thr Thr Leu 1325 1330 1335 Ser Arg Thr Asn Lys Lys Arg Ser Trp Pro Leu Asn Glu Ala Ile 1340 1345 1350 Met Ala Val Gly Met Val Ser Ile Leu Ala Ser Ser Leu Leu Lys 1355 1360 1365 Asn Asp Ile Pro Met Thr Gly Pro Leu Val Ala Gly Gly Leu Leu 1370 1375 1380 Thr Val Cys Tyr Val Leu Thr Gly Arg Ser Ala Asp Leu Glu Leu 1385 1390 1395 Glu Arg Ala Thr Asp Val Lys Trp Glu Asp Gln Ala Glu Ile Ser 1400 1405 1410 Gly Ser Ser Pro Ile Leu Ser Ile Thr Ile Ser Glu Asp Gly Ser 1415 1420 1425 Met Ser Ile Lys Asn Glu Glu Glu Glu Gln Thr Leu Thr Ile Leu 1430 1435 1440 Ile Arg Thr Gly Leu Leu Val Ile Ser Gly Leu Phe Pro Ile Ser 1445 1450 1455 Ile Pro Xaa Thr Ala Ala Ala Trp Tyr Leu Trp Glu Val Lys Lys 1460 1465 1470 Gln Arg Ala Gly Val Leu Trp Asp Val Pro Ser Pro Pro Pro Met 1475 1480 1485 Gly Lys Ala Glu Leu Glu Asp Gly Ala Tyr Arg Ile Lys Gln Lys 1490 1495 1500 Gly Ile Leu Gly Tyr Ser Gln Ile Gly Ala Gly Val Tyr Lys Glu 1505 1510 1515 Gly Thr Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu 1520 1525 1530 Met His Lys Gly Lys Arg Ile Glu Pro Ser Trp Ala Asp Val Lys 1535 1540 1545 Lys Asp Leu Ile Ser Tyr Gly Gly Gly Trp Lys Leu Glu Gly Glu 1550 1555 1560 Trp Lys Glu Gly Glu Glu Val Gln Val Leu Ala Leu Glu Pro Gly 1565 1570 1575 Lys Asn Pro Arg Ala Val Gln Thr Lys Pro Gly Leu Phe Lys Thr 1580 1585 1590 Asn Thr Gly Thr Ile Gly Ala Val Ser Leu Asp Phe Ser Pro Gly 1595 1600 1605 Thr Ser Gly Ser Pro Ile Ile Asp Lys Lys Gly Lys Val Val Gly 1610 1615 1620 Leu Tyr Gly Asn Gly Val Val Thr Arg Ser Gly Ala Tyr Val Ser 1625 1630 1635 Ala Ile Ala Gln Thr Glu Lys Ser Ile Glu Asp Asn Pro Glu Ile 1640 1645 1650 Lys Asp Asp Ile Phe Arg Lys Arg Arg Leu Thr Ile Met Asp Leu 1655 1660 1665 His Pro Gly Ala Gly Lys Thr Lys Arg Tyr Leu Pro Ala Ile Val 1670 1675 1680 Arg Glu Ala Ile Lys Arg Gly Leu Arg Thr Leu Ile Leu Ala Pro 1685 1690 1695 Thr Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu 1700 1705 1710 Pro Ile Arg Tyr Gln Thr Pro Ala Ile Arg Ala Glu His Thr Gly 1715 1720 1725 Arg Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met Arg 1730 1735 1740 Leu Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Leu Ile Ile Met 1745 1750 1755 Asp Glu Ala His Phe Thr Asp Pro Ala Ser Ile Ala Ala Arg Gly 1760 1765 1770 Tyr Ile Ser Thr Arg Val Glu Met Gly Glu Ala Ala Gly Ile Phe 1775 1780 1785 Met Thr Ala Thr Pro Pro Gly Ser Arg Asp Pro Phe Pro Gln Ser 1790 1795 1800 Asn Ala Pro Ile Ile Asp Glu Glu Arg Glu Ile Pro Glu Arg Ser 1805 1810 1815 Trp Asn Ser Gly His Glu Trp Val Thr Asp Phe Lys Gly Lys Thr 1820 1825 1830 Val Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Ala 1835 1840 1845 Cys Leu Arg Lys Asn Gly Lys Lys Val Ile Gln Leu Ser Arg Lys 1850 1855 1860 Thr Phe Asp Ser Glu Tyr Val Lys Thr Arg Thr Asn Asp Trp Asp 1865 1870 1875 Phe Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys 1880 1885 1890 Ala Glu Arg Val Ile Asp Pro Arg Arg Cys Met Lys Pro Val Ile 1895 1900 1905 Leu Thr Asp Gly Glu Glu Arg Val Ile Leu Ala Gly Pro Met Pro 1910 1915 1920 Val Thr His Ser Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg 1925 1930 1935 Asn Pro Lys Asn Glu Asn Asp Gln Tyr Ile Tyr Met Gly Glu Pro 1940 1945 1950 Leu Glu Asn Asp Glu Asp Cys Ala His Trp Lys Glu Ala Lys Met 1955 1960 1965 Leu Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ser Met 1970 1975 1980 Phe Glu Pro Glu Arg Glu Lys Val Asp Ala Ile Asp Gly Glu Tyr 1985 1990 1995 Arg Leu Arg Gly Glu Ala Arg Lys Thr Phe Val Asp Leu Met Arg 2000 2005 2010 Arg Gly Asp Leu Pro Val Trp Leu Ala Tyr Lys Val Ala Ala Glu 2015 2020 2025 Gly Ile Asn Tyr Ala Asp Arg Arg Trp Cys Phe Asp Gly Ile Lys 2030 2035 2040 Asn Asn Gln Ile Leu Glu Glu Asn Val Glu Val Glu Ile Trp Thr 2045 2050 2055 Lys Glu Gly Glu Arg Lys Lys Leu Lys Pro Arg Trp Leu Asp Ala 2060 2065 2070 Arg Ile Tyr Ser Asp Pro Leu Ala Leu Lys Glu Phe Lys Glu Phe 2075 2080 2085 Ala Ala Gly Arg Lys Ser Leu Thr Leu Asn Leu Ile Thr Glu Met 2090 2095 2100 Gly Arg Leu Pro Thr Phe Met Thr Gln Lys Thr Arg Asp Ala Leu 2105 2110 2115 Asp Asn Leu Ala Val Leu His Thr Ala Glu Ala Gly Gly Arg Ala 2120 2125 2130 Tyr Asn His Ala Leu Ser Glu Leu Pro Glu Thr Leu Glu Thr Leu 2135 2140 2145 Leu Leu Leu Thr Leu Leu Ala Thr Val Thr Gly Gly Ile Phe Leu 2150 2155 2160 Phe Leu Met Ser Gly Arg Gly Ile Gly Lys Met Thr Leu Gly Met 2165 2170 2175 Cys Cys Ile Ile Thr Ala Ser Ile Leu Leu Trp Xaa Ala Gln Ile 2180 2185 2190 Gln Pro His Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe Leu 2195 2200 2205 Ile Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln 2210 2215 2220 Asp Asn Gln Leu Thr Tyr Val Val Ile Ala Ile Leu Thr Val Val 2225 2230 2235 Ala Ala Thr Met Ala Asn Glu Met Gly Phe Leu Glu Lys Thr Lys 2240 2245 2250 Lys Asp Leu Gly Leu Gly Ser Ile Ala Thr Gln Gln Pro Glu Ser 2255 2260 2265 Asn Ile Leu Asp Ile Asp Leu Arg Pro Ala Ser Ala Trp Thr Leu 2270 2275 2280 Tyr Ala Val Ala Thr Thr Phe Val Thr Pro Met Leu Arg His Ser 2285 2290 2295 Ile Glu Asn Ser Ser Val Asn Val Ser Leu Thr Ala Ile Ala Asn 2300 2305 2310 Gln Ala Thr Val Leu Met Gly Leu Gly Lys Gly Trp Pro Leu Ser 2315 2320 2325 Lys Met Asp Ile Gly Val Pro Leu Leu Ala Ile Gly Cys Tyr Ser 2330 2335 2340 Gln Val Asn Xaa Ile Thr Leu Thr Ala Ala Leu Leu Leu Leu Val 2345 2350 2355 Ala His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr 2360 2365 2370 Arg Glu Ala Gln Lys Arg Ala Ala Ala Gly Ile Met Lys Asn Pro 2375 2380 2385 Thr Val Asp Gly Ile Thr Val Ile Asp Leu Asp Pro Ile Pro Tyr 2390 2395 2400 Asp Pro Lys Phe Glu Lys Gln Leu Gly Gln Val Met Leu Leu Ile 2405 2410 2415 Leu Cys Val Thr Gln Val Leu Met Met Arg Thr Thr Trp Ala Leu 2420 2425 2430 Cys Glu Ala Leu Thr Leu Ala Thr Gly Pro Ile Ser Thr Leu Trp 2435 2440 2445 Glu Gly Asn Pro Gly Arg Phe Trp Asn Thr Thr Ile Ala Val Ser 2450 2455 2460 Met Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu 2465 2470 2475 Leu Phe Ser Ile Met Lys Asn Thr Thr Asn Thr Arg Arg Gly Thr 2480 2485 2490 Gly Asn Ile Gly Glu Thr Leu Gly Glu Lys Trp Lys Ser Arg Leu 2495 2500 2505 Asn Ala Leu Gly Lys Ser Glu Phe Gln Ile Tyr Lys Lys Ser Gly 2510 2515 2520 Ile Gln Glu Val Asp Arg Thr Leu Ala Lys Glu Gly Ile Lys Arg 2525 2530 2535 Gly Glu Thr Asp His His Ala Val Ser Arg Gly Ser Ala Lys Leu 2540 2545 2550 Arg Trp Phe Val Glu Arg Asn Met Val Thr Pro Glu Gly Lys Val 2555 2560 2565 Val Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Cys Gly 2570 2575 2580 Gly Leu Lys Asn Val Arg Glu Val Lys Gly Leu Thr Lys Gly Gly 2585 2590 2595 Pro Gly His Glu Glu Pro Ile Pro Met Ser Thr Tyr Gly Trp Asn 2600 2605 2610 Leu Val Arg Leu Gln Ser Gly Val Asp Val Phe Phe Ile Pro Pro 2615 2620 2625 Glu Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Pro 2630 2635 2640 Asn Pro Thr Val Glu Ala Gly Arg Thr Leu Arg Val Leu Asn Leu 2645 2650 2655 Val Glu Asn Trp Leu Asn Asn Asn Thr Gln Phe Cys Ile Lys Val 2660 2665 2670 Leu Asn Pro Tyr Met Pro Ser Val Ile Glu Lys Met Glu Ala Leu 2675 2680 2685 Gln Arg Lys Tyr Gly Gly Ala Leu Val Arg Asn Pro Leu Ser Arg 2690 2695 2700 Asn Ser Thr His Glu Met Tyr Trp Val Ser Asn Ala Ser Gly Asn 2705 2710 2715 Ile Val Ser Ser Val Asn Met Ile Ser Arg Met Leu Ile Asn Arg 2720 2725 2730 Phe Thr Met Arg His Lys Lys Ala Thr Tyr Glu Pro Asp Val Asp 2735 2740 2745 Leu Gly Ser Gly Thr Arg Asn Ile Gly Val Glu Ser Glu Ile Pro 2750 2755 2760 Asn Leu Asp Ile Ile Gly Lys Arg Ile Glu Lys Ile Lys Gln Glu 2765 2770 2775 His Glu Thr Ser Trp His Tyr Asp Gln Asp His Pro Tyr Lys Thr 2780 2785 2790 Trp Ala Tyr His Gly Ser Tyr Glu Thr Lys Gln Thr Gly Ser Ala 2795 2800 2805 Ser Ser Met Val Asn Gly Val Val Arg Leu Leu Thr Lys Pro Trp 2810 2815 2820 Asp Val Leu Pro Xaa Val Thr Gln Met Ala Met Thr Asp Thr Thr 2825 2830 2835 Pro Phe Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg 2840 2845 2850 Thr Gln Glu Pro Lys Glu Gly Thr Lys Lys Leu Met Arg Ile Thr 2855 2860 2865 Ala Glu Trp Leu Trp Lys Glu Leu Gly Lys Lys Lys Thr Pro Arg 2870 2875 2880 Met Cys Thr Arg Glu Glu Phe Thr Arg Lys Val Arg Ser Asn Ala 2885 2890 2895 Ala Leu Gly Ala Ile Phe Thr Asp Glu Asn Lys Trp Lys Ser Ala 2900 2905 2910 Arg Glu Ala Val Glu Asp Ser Arg Phe Trp Glu Leu Val Asp Lys 2915 2920 2925 Glu Arg Asn Leu His Leu Glu Gly Lys Cys Glu Thr Cys Val Tyr 2930 2935 2940 Asn Met Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Lys 2945 2950 2955 Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg 2960 2965 2970 Phe Leu Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp 2975 2980 2985 Phe Ser Arg Glu Asn Ser Leu Ser Gly Val Glu Gly Glu Gly Leu 2990 2995 3000 His Lys Leu Gly Tyr Ile Leu Arg Asp Val Ser Lys Lys Glu Gly 3005 3010 3015 Gly Xaa Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile 3020 3025 3030 Thr Leu Glu Asp Leu Lys Asn Glu Glu Met Val Thr Asn His Met 3035 3040 3045 Glu Gly Glu His Lys Lys Leu Ala Glu Ala Ile Phe Lys Leu Thr 3050 3055 3060 Tyr Gln Asn Lys Val Val Arg Val Gln Arg Pro Thr Pro Arg Gly 3065 3070 3075 Thr Val Met Asp Ile Ile Ser Arg Arg Asp Gln Arg Gly Ser Gly 3080 3085 3090 Gln Val Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Ala 3095 3100 3105 Gln Leu Ile Arg Gln Met Glu Gly Glu Gly Val Phe Lys Asn Ile 3110 3115 3120 Gln His Leu Thr Val Thr Glu Glu Val Ala Val Gln Asn Trp Leu 3125 3130 3135 Ala Arg Val Gly Arg Glu Arg Leu Ser Arg Met Ala Ile Ser Gly 3140 3145 3150 Asp Asp Cys Val Val Lys Pro Leu Asp Asp Arg Phe Ala Asn Ala 3155 3160 3165 Leu Thr Ala Leu Asn Asp Met Gly Lys Ile Arg Lys Asp Ile Gln 3170 3175 3180 Gln Trp Glu Pro Ser Lys Gly Trp Asn Asp Trp Thr Gln Val Pro 3185 3190 3195 Phe Cys Ser His His Phe His Glu Leu Ile Met Lys Asp Gly Arg 3200 3205 3210 Val Leu Val Val Pro Cys Arg Asn Gln Asp Glu Leu Ile Gly Arg 3215 3220 3225 Ala Arg Ile Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala 3230 3235 3240 Cys Leu Gly Lys Ser Tyr Ala Gln Met Trp Ser Leu Met Tyr Phe 3245 3250 3255 His Arg Arg Asp Leu Arg Leu Ala Ala Asn Ala Ile Cys Ser Ala 3260 3265 3270 Val Pro Ser His Trp Val Pro Thr Ser Arg Thr Thr Trp Ser Ile 3275 3280 3285 His Ala Lys His Glu Trp Met Thr Thr Glu Asp Met Leu Thr Val 3290 3295 3300 Trp Asn Arg Val Trp Ile Arg Glu Asn Pro Trp Met Glu Asp Lys 3305 3310 3315 Thr Pro Val Glu Ser Trp Glu Glu Ile Pro Tyr Leu Gly Lys Arg 3320 3325 3330 Glu Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ser Arg Ala 3335 3340 3345 Thr Trp Ala Lys Asn Ile Gln Ala Ala Ile Asn Gln Val Arg Ser 3350 3355 3360 Leu Ile Gly Asn Glu Glu Tyr Thr Asp Tyr Met Pro Ser Met Lys 3365 3370 3375 Arg Phe Arg Arg Glu Glu Glu Glu Ala Gly Val Leu Trp 3380 3385 3390 <210> 11 <211> 3390 <212> PRT <213> Dengue virus type 3 <220> <221> VARIANT <222> (764)..(764) <223> Xaa stands for Thr or Ile <400> 11 Met Asn Asn Gln Arg Lys Lys Thr Gly Lys Pro Ser Ile Asn Met Leu 1 5 10 15 Lys Arg Val Arg Asn Arg Val Ser Thr Gly Ser Gln Leu Ala Lys Arg 20 25 30 Phe Ser Arg Gly Leu Leu Asn Gly Gln Gly Pro Met Lys Leu Val Met 35 40 45 Ala Phe Ile Ala Phe Leu Arg Phe Leu Ala Ile Pro Pro Thr Ala Gly 50 55 60 Val Leu Ala Arg Trp Gly Thr Phe Lys Lys Ser Gly Ala Ile Lys Val 65 70 75 80 Leu Lys Gly Phe Lys Lys Glu Ile Ser Asn Met Leu Ser Ile Ile Asn 85 90 95 Lys Arg Lys Lys Thr Ser Leu Cys Leu Met Met Met Leu Pro Ala Thr 100 105 110 Leu Ala Phe His Leu Thr Ser Arg Asp Gly Glu Pro Arg Met Ile Val 115 120 125 Gly Lys Asn Glu Arg Gly Lys Ser Leu Leu Phe Lys Thr Ala Ser Gly 130 135 140 Ile Asn Met Cys Thr Leu Ile Ala Met Asp Leu Gly Glu Met Cys Asp 145 150 155 160 Asp Thr Val Thr Tyr Lys Cys Pro His Ile Thr Glu Val Glu Pro Glu 165 170 175 Asp Ile Asp Cys Trp Cys Asn Leu Thr Ser Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Asn Gln Ala Gly Glu His Arg Arg Asp Lys Arg Ser Val Ala 195 200 205 Leu Ala Pro His Val Gly Met Gly Leu Asp Thr Arg Thr Gln Thr Trp 210 215 220 Met Ser Ala Glu Gly Ala Trp Arg Gln Val Glu Lys Val Glu Thr Trp 225 230 235 240 Ala Leu Arg His Pro Gly Phe Thr Ile Leu Ala Leu Phe Leu Ala His 245 250 255 Tyr Ile Gly Thr Ser Leu Thr Gln Lys Val Val Ile Phe Ile Leu Leu 260 265 270 Met Leu Val Thr Pro Ser Met Thr Met Arg Cys Val Gly Val Gly Asn 275 280 285 Arg Asp Phe Val Glu Gly Leu Ser Gly Ala Thr Trp Val Asp Val Val 290 295 300 Leu Glu His Gly Gly Cys Val Thr Thr Met Ala Lys Asn Lys Pro Thr 305 310 315 320 Leu Asp Ile Glu Leu Gln Lys Thr Glu Ala Thr Gln Leu Ala Thr Leu 325 330 335 Arg Lys Leu Cys Ile Glu Gly Lys Ile Thr Asn Ile Thr Thr Asp Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Ala Ile Leu Pro Glu Glu Gln Asp Gln 355 360 365 Asn Tyr Val Cys Lys His Thr Tyr Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Gln 385 390 395 400 Cys Leu Glu Ser Ile Glu Gly Lys Val Val Gln His Glu Asn Leu Lys 405 410 415 Tyr Thr Val Ile Ile Thr Val His Thr Gly Asp Gln His Gln Val Gly 420 425 430 Asn Glu Thr Gln Gly Val Thr Ala Glu Ile Thr Pro Gln Ala Ser Thr 435 440 445 Ala Glu Ala Ile Leu Pro Glu Tyr Gly Thr Leu Gly Leu Glu Cys Ser 450 455 460 Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Ile Ser Leu Thr Met Lys 465 470 475 480 Asn Lys Ala Trp Met Val His Arg Gln Trp Phe Phe Asp Leu Pro Leu 485 490 495 Pro Trp Thr Ser Gly Ala Thr Ala Glu Thr Pro Thr Trp Asn Arg Lys 500 505 510 Glu Leu Leu Val Thr Phe Lys Asn Ala His Ala Lys Lys Gln Glu Val 515 520 525 Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu Thr Gly 530 535 540 Ala Thr Glu Ile Gln Thr Ser Gly Gly Thr Ser Ile Phe Ala Gly His 545 550 555 560 Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Glu Leu Lys Gly Met Ser 565 570 575 Tyr Ala Met Cys Leu Gly Ser Phe Val Leu Lys Lys Glu Val Ser Glu 580 585 590 Thr Gln His Gly Thr Ile Leu Ile Lys Val Glu Tyr Lys Gly Glu Asp 595 600 605 Ala Pro Cys Lys Ile Pro Phe Ser Thr Glu Asp Gly Gln Gly Lys Ala 610 615 620 His Asn Gly Arg Leu Ile Thr Ala Asn Pro Val Val Thr Lys Lys Glu 625 630 635 640 Glu Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser Asn Ile 645 650 655 Val Ile Gly Ile Gly Asp Lys Ala Leu Lys Ile Asn Trp Tyr Lys Lys 660 665 670 Gly Ser Ser Ile Gly Lys Met Phe Glu Ala Thr Ala Arg Gly Val Arg 675 680 685 Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly 690 695 700 Gly Val Leu Asn Ser Leu Gly Lys Met Val His Gln Ile Phe Gly Ser 705 710 715 720 Ala Tyr Thr Ala Leu Phe Gly Gly Val Ser Trp Met Met Lys Ile Gly 725 730 735 Ile Gly Val Leu Leu Thr Trp Ile Gly Leu Asn Ser Lys Asn Thr Ser 740 745 750 Met Ser Phe Ser Cys Ile Ala Ile Gly Ile Ile Xaa Leu Tyr Leu Gly 755 760 765 Ala Val Val Gln Ala Asp Met Gly Cys Val Ile Asn Trp Lys Gly Lys 770 775 780 Glu Leu Lys Cys Gly Ser Gly Ile Phe Val Thr Asn Glu Val His Thr 785 790 795 800 Trp Thr Glu Gln Tyr Lys Phe Gln Ala Asp Ser Pro Lys Arg Leu Ala 805 810 815 Thr Ala Ile Ala Gly Ala Trp Glu Asn Gly Val Cys Gly Ile Arg Ser 820 825 830 Thr Thr Arg Met Glu Asn Leu Leu Trp Lys Gln Ile Ala Asn Glu Leu 835 840 845 Asn Tyr Ile Leu Trp Glu Asn Asn Ile Lys Leu Thr Val Val Val Gly 850 855 860 Asp Ile Thr Gly Val Leu Glu Gln Gly Lys Arg Thr Leu Thr Pro Gln 865 870 875 880 Pro Met Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala Lys Ile 885 890 895 Val Thr Ala Glu Thr Gln Asn Ser Ser Phe Ile Ile Asp Gly Pro Ser 900 905 910 Thr Pro Glu Cys Pro Ser Ala Ser Arg Ala Trp Asn Val Trp Glu Val 915 920 925 Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu Lys Leu 930 935 940 Arg Glu Val Tyr Thr Gln Leu Cys Asp His Arg Leu Met Ser Ala Ala 945 950 955 960 Val Lys Asp Glu Arg Ala Val His Ala Asp Met Gly Tyr Trp Ile Glu 965 970 975 Ser Gln Lys Asn Gly Ser Trp Lys Leu Glu Lys Ala Ser Phe Ile Glu 980 985 990 Val Lys Thr Cys Thr Trp Pro Lys Ser His Thr Leu Trp Ser Asn Gly 995 1000 1005 Val Leu Glu Ser Asp Met Ile Ile Pro Lys Ser Leu Ala Gly Pro 1010 1015 1020 Ile Ser Gln His Asn His Arg Pro Gly Tyr His Thr Gln Thr Ala 1025 1030 1035 Gly Pro Trp His Leu Gly Lys Leu Glu Leu Asp Phe Asn Tyr Cys 1040 1045 1050 Glu Gly Thr Thr Val Val Ile Ser Glu Asn Cys Gly Thr Arg Gly 1055 1060 1065 Pro Ser Leu Arg Thr Thr Thr Val Ser Gly Lys Leu Ile His Glu 1070 1075 1080 Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Tyr Met Gly 1085 1090 1095 Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Ile Asn Glu 1100 1105 1110 Lys Glu Glu Asn Met Val Lys Ser Leu Ala Ser Ala Gly Ser Gly 1115 1120 1125 Lys Val Asp Asn Phe Thr Met Gly Val Leu Cys Leu Ala Ile Leu 1130 1135 1140 Phe Glu Glu Val Met Arg Gly Lys Phe Gly Lys Lys His Met Ile 1145 1150 1155 Ala Gly Val Leu Phe Thr Phe Val Leu Leu Leu Ser Gly Gln Ile 1160 1165 1170 Thr Trp Arg Asp Met Ala His Thr Leu Ile Met Ile Gly Ser Asn 1175 1180 1185 Ala Ser Asp Arg Met Gly Met Gly Val Thr Tyr Leu Ala Leu Ile 1190 1195 1200 Ala Thr Phe Lys Ile Gln Pro Leu Leu Ala Leu Gly Phe Phe Leu 1205 1210 1215 Arg Lys Leu Thr Ser Arg Glu Asn Leu Leu Leu Gly Val Gly Leu 1220 1225 1230 Ala Met Ala Thr Thr Leu Arg Leu Pro Glu Asp Ile Glu Gln Met 1235 1240 1245 Ala Asn Gly Ile Ala Leu Gly Leu Met Ala Leu Lys Leu Ile Thr 1250 1255 1260 Gln Phe Glu Thr Tyr Gln Leu Trp Thr Ala Leu Val Ser Leu Thr 1265 1270 1275 Cys Ser Asn Thr Ile Phe Thr Leu Thr Val Ala Trp Arg Thr Ala 1280 1285 1290 Thr Leu Ile Leu Ala Gly Ile Ser Leu Leu Pro Val Cys Gln Ser 1295 1300 1305 Ser Ser Met Arg Lys Thr Asp Trp Leu Pro Met Thr Val Ala Ala 1310 1315 1320 Met Gly Ala Gln Pro Leu Pro Leu Phe Ile Phe Ser Leu Lys Asp 1325 1330 1335 Thr Leu Lys Arg Arg Ser Trp Pro Leu Asn Glu Gly Val Met Ala 1340 1345 1350 Val Gly Leu Val Ser Ile Leu Ala Ser Ser Leu Leu Arg Asn Asp 1355 1360 1365 Val Pro Met Ala Gly Pro Leu Val Ala Gly Gly Leu Leu Ile Ala 1370 1375 1380 Cys Tyr Val Ile Thr Gly Thr Ser Ala Asp Leu Thr Val Glu Lys 1385 1390 1395 Ala Ala Asp Val Thr Trp Glu Glu Glu Ala Glu Gln Thr Gly Val 1400 1405 1410 Ser His Asn Leu Met Val Thr Val Asp Asp Asp Gly Thr Met Arg 1415 1420 1425 Ile Lys Asp Asp Glu Thr Glu Asn Ile Leu Thr Val Leu Leu Lys 1430 1435 1440 Thr Ala Leu Leu Ile Val Ser Gly Ile Phe Pro Tyr Ser Ile Pro 1445 1450 1455 Ala Thr Leu Leu Val Trp His Thr Trp Gln Lys Gln Thr Gln Arg 1460 1465 1470 Ser Gly Val Leu Trp Asp Val Pro Ser Pro Pro Glu Thr Gln Lys 1475 1480 1485 Ala Glu Leu Glu Glu Gly Val Tyr Arg Ile Lys Gln Gln Gly Ile 1490 1495 1500 Phe Gly Lys Thr Gln Val Gly Val Gly Val Gln Lys Glu Gly Val 1505 1510 1515 Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu Thr His 1520 1525 1530 Asn Gly Lys Arg Leu Glu Pro Asn Trp Ala Ser Val Lys Lys Asp 1535 1540 1545 Leu Ile Ser Tyr Gly Gly Gly Trp Arg Leu Ser Ala Gln Trp Lys 1550 1555 1560 Lys Gly Glu Glu Val Gln Val Ile Ala Val Glu Pro Gly Lys Asn 1565 1570 1575 Pro Lys Asn Phe Gln Thr Met Pro Gly Ile Phe Gln Thr Thr Thr 1580 1585 1590 Gly Glu Ile Gly Ala Ile Ala Leu Asp Phe Lys Pro Gly Thr Ser 1595 1600 1605 Gly Ser Pro Ile Ile Asn Arg Glu Gly Lys Val Val Gly Leu Tyr 1610 1615 1620 Gly Asn Gly Val Val Thr Lys Asn Gly Gly Tyr Val Ser Gly Ile 1625 1630 1635 Ala Gln Thr Asn Ala Glu Pro Asp Gly Pro Thr Pro Glu Leu Glu 1640 1645 1650 Glu Glu Met Phe Lys Lys Arg Asn Leu Thr Ile Met Asp Leu His 1655 1660 1665 Pro Gly Ser Gly Lys Thr Arg Lys Tyr Leu Pro Ala Ile Val Arg 1670 1675 1680 Glu Ala Ile Lys Arg Arg Leu Arg Thr Leu Ile Leu Ala Pro Thr 1685 1690 1695 Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Lys Gly Leu Pro 1700 1705 1710 Ile Arg Tyr Gln Thr Thr Ala Thr Lys Ser Glu His Thr Gly Arg 1715 1720 1725 Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met Arg Leu 1730 1735 1740 Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Leu Ile Ile Met Asp 1745 1750 1755 Glu Ala His Phe Thr Asp Pro Ala Ser Ile Ala Ala Arg Gly Tyr 1760 1765 1770 Ile Ser Thr Arg Val Gly Met Gly Glu Ala Ala Ala Ile Phe Met 1775 1780 1785 Thr Ala Thr Pro Pro Gly Thr Ala Asp Ala Phe Pro Gln Ser Asn 1790 1795 1800 Ala Pro Ile Gln Asp Glu Glu Arg Asp Ile Pro Glu Arg Ser Trp 1805 1810 1815 Asn Ser Gly Asn Glu Trp Ile Thr Asp Phe Val Gly Lys Thr Val 1820 1825 1830 Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Asn Cys 1835 1840 1845 Leu Arg Lys Asn Gly Lys Lys Val Ile Gln Leu Ser Arg Lys Thr 1850 1855 1860 Phe Asp Thr Glu Tyr Gln Lys Thr Lys Leu Asn Asp Trp Asp Phe 1865 1870 1875 Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys Ala 1880 1885 1890 Asp Arg Val Ile Asp Pro Arg Arg Cys Leu Lys Pro Val Ile Leu 1895 1900 1905 Thr Asp Gly Pro Glu Arg Val Ile Leu Ala Gly Pro Met Pro Val 1910 1915 1920 Thr Val Ala Ser Ala Ala Gln Arg Arg Gly Arg Val Gly Arg Asn 1925 1930 1935 Pro Gln Lys Glu Asn Asp Gln Tyr Ile Phe Met Gly Gln Pro Leu 1940 1945 1950 Asn Asn Asp Glu Asp His Ala His Trp Thr Glu Ala Lys Met Leu 1955 1960 1965 Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ala Leu Phe 1970 1975 1980 Glu Pro Glu Arg Glu Lys Ser Ala Ala Ile Asp Gly Glu Tyr Arg 1985 1990 1995 Leu Lys Gly Glu Ser Arg Lys Thr Phe Val Glu Leu Met Arg Arg 2000 2005 2010 Gly Asp Leu Pro Val Trp Leu Ala His Lys Val Ala Ser Glu Gly 2015 2020 2025 Ile Lys Tyr Thr Asp Arg Lys Trp Cys Phe Asp Gly Glu Arg Asn 2030 2035 2040 Asn Gln Ile Leu Glu Glu Asn Met Asp Val Glu Ile Trp Thr Lys 2045 2050 2055 Glu Gly Glu Lys Lys Lys Leu Arg Pro Arg Trp Leu Asp Ala Arg 2060 2065 2070 Thr Tyr Ser Asp Pro Leu Ala Leu Lys Glu Phe Lys Asp Phe Ala 2075 2080 2085 Ala Gly Arg Lys Ser Ile Ala Leu Asp Leu Val Thr Glu Ile Gly 2090 2095 2100 Arg Val Pro Ser His Leu Ala His Arg Thr Arg Asn Ala Leu Asp 2105 2110 2115 Asn Leu Val Met Leu His Thr Ser Glu His Gly Gly Arg Ala Tyr 2120 2125 2130 Arg His Ala Val Glu Glu Leu Pro Glu Thr Met Glu Thr Leu Leu 2135 2140 2145 Leu Leu Gly Leu Met Ile Leu Leu Thr Gly Gly Ala Met Leu Phe 2150 2155 2160 Leu Ile Ser Gly Lys Gly Ile Gly Lys Thr Ser Ile Gly Leu Ile 2165 2170 2175 Cys Val Ile Ala Ser Ser Gly Met Leu Trp Met Ala Asp Val Pro 2180 2185 2190 Leu Gln Trp Ile Ala Ser Ala Ile Val Leu Glu Phe Phe Met Met 2195 2200 2205 Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln Asp 2210 2215 2220 Asn Gln Leu Ala Tyr Val Val Ile Gly Ile Leu Thr Leu Ala Ala 2225 2230 2235 Ile Val Ala Ala Asn Glu Met Gly Leu Leu Glu Thr Thr Lys Arg 2240 2245 2250 Asp Leu Gly Met Ser Lys Glu Pro Gly Val Val Ser Pro Thr Ser 2255 2260 2265 Tyr Leu Asp Val Asp Leu His Pro Ala Ser Ala Trp Thr Leu Tyr 2270 2275 2280 Ala Val Ala Thr Thr Val Ile Thr Pro Met Leu Arg His Thr Ile 2285 2290 2295 Glu Asn Ser Thr Ala Asn Val Ser Leu Ala Ala Ile Ala Asn Gln 2300 2305 2310 Ala Val Val Leu Met Gly Leu Asp Lys Gly Trp Pro Ile Ser Lys 2315 2320 2325 Met Asp Leu Gly Val Pro Leu Leu Ala Leu Gly Cys Tyr Ser Gln 2330 2335 2340 Val Asn Pro Leu Thr Leu Ala Ala Ala Val Leu Leu Leu Val Thr 2345 2350 2355 His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr Arg 2360 2365 2370 Glu Ala Gln Lys Arg Thr Ala Ala Gly Ile Met Lys Asn Pro Thr 2375 2380 2385 Val Asp Gly Ile Met Thr Ile Asp Leu Asp Pro Val Ile Tyr Asp 2390 2395 2400 Ser Lys Phe Glu Lys Gln Leu Gly Gln Val Met Leu Leu Val Leu 2405 2410 2415 Cys Ala Val Gln Leu Leu Leu Met Arg Thr Ser Trp Ala Leu Cys 2420 2425 2430 Glu Val Leu Thr Leu Ala Thr Gly Pro Ile Thr Thr Leu Trp Glu 2435 2440 2445 Gly Ser Pro Gly Lys Phe Trp Asn Thr Thr Ile Ala Val Ser Met 2450 2455 2460 Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu Ala 2465 2470 2475 Phe Ser Ile Met Lys Ser Val Gly Thr Gly Lys Arg Gly Thr Gly 2480 2485 2490 Ser Gln Gly Glu Thr Leu Gly Glu Lys Trp Lys Lys Lys Leu Asn 2495 2500 2505 Gln Leu Ser Arg Lys Glu Phe Asp Leu Tyr Lys Lys Ser Gly Ile 2510 2515 2520 Thr Glu Val Asp Arg Thr Glu Ala Lys Glu Gly Leu Lys Arg Gly 2525 2530 2535 Glu Ile Thr His His Ala Val Ser Arg Gly Ser Ala Lys Leu Gln 2540 2545 2550 Trp Phe Val Glu Arg Asn Met Val Ile Pro Glu Gly Arg Val Ile 2555 2560 2565 Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Cys Ala Gly 2570 2575 2580 Leu Lys Lys Val Thr Glu Val Arg Gly Tyr Thr Lys Gly Gly Pro 2585 2590 2595 Gly His Glu Glu Pro Val Pro Met Ser Thr Tyr Gly Trp Asn Ile 2600 2605 2610 Val Lys Leu Met Ser Gly Lys Asp Val Phe Tyr Leu Pro Pro Glu 2615 2620 2625 Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Pro Ser 2630 2635 2640 Pro Thr Val Glu Glu Ser Arg Thr Ile Arg Val Leu Lys Met Val 2645 2650 2655 Glu Pro Trp Leu Lys Asn Asn Gln Phe Cys Ile Lys Val Leu Asn 2660 2665 2670 Pro Tyr Met Pro Thr Val Ile Glu His Leu Glu Arg Leu Gln Arg 2675 2680 2685 Lys His Gly Gly Met Leu Val Arg Asn Pro Leu Ser Arg Asn Ser 2690 2695 2700 Thr His Glu Met Tyr Trp Ile Ser Asn Gly Thr Gly Asn Ile Val 2705 2710 2715 Ser Ser Val Asn Met Val Ser Arg Leu Leu Leu Asn Arg Phe Thr 2720 2725 2730 Met Thr His Arg Arg Pro Thr Ile Glu Lys Asp Val Asp Leu Gly 2735 2740 2745 Ala Gly Thr Arg His Val Asn Ala Glu Pro Glu Thr Pro Asn Met 2750 2755 2760 Asp Val Ile Gly Glu Arg Ile Lys Arg Ile Lys Glu Glu His Ser 2765 2770 2775 Ser Thr Trp His Tyr Asp Asp Glu Asn Pro Tyr Lys Thr Trp Ala 2780 2785 2790 Tyr His Gly Ser Tyr Glu Val Lys Ala Thr Gly Ser Ala Ser Ser 2795 2800 2805 Met Ile Asn Gly Val Val Lys Leu Leu Thr Lys Pro Trp Asp Val 2810 2815 2820 Val Pro Met Val Thr Gln Met Ala Met Thr Asp Thr Thr Pro Phe 2825 2830 2835 Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg Thr Pro 2840 2845 2850 Arg Pro Met Pro Gly Thr Arg Lys Val Met Glu Ile Thr Ala Glu 2855 2860 2865 Trp Leu Trp Arg Thr Leu Gly Arg Asn Lys Arg Pro Arg Leu Cys 2870 2875 2880 Thr Arg Glu Glu Phe Thr Lys Lys Val Arg Thr Asn Ala Ala Met 2885 2890 2895 Gly Ala Val Phe Thr Glu Glu Asn Gln Trp Asp Ser Ala Arg Ala 2900 2905 2910 Ala Val Glu Asp Glu Glu Phe Trp Lys Leu Val Asp Arg Glu Arg 2915 2920 2925 Glu Leu His Lys Leu Gly Lys Cys Gly Ser Cys Val Tyr Asn Met 2930 2935 2940 Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Lys Ala Lys 2945 2950 2955 Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg Tyr Leu 2960 2965 2970 Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp Phe Ser 2975 2980 2985 Arg Glu Asn Ser Tyr Ser Gly Val Glu Gly Glu Gly Leu His Lys 2990 2995 3000 Leu Gly Tyr Ile Leu Arg Asp Ile Ser Lys Ile Pro Gly Gly Ala 3005 3010 3015 Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile Thr Glu 3020 3025 3030 Asp Asp Leu His Asn Glu Glu Lys Ile Thr Gln Gln Met Asp Pro 3035 3040 3045 Glu His Arg Gln Leu Ala Asn Ala Ile Phe Lys Leu Thr Tyr Gln 3050 3055 3060 Asn Lys Val Val Lys Val Gln Arg Pro Thr Pro Lys Gly Thr Val 3065 3070 3075 Met Asp Ile Ile Ser Arg Lys Asp Gln Arg Gly Ser Gly Gln Val 3080 3085 3090 Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Ala Gln Leu 3095 3100 3105 Ile Arg Gln Met Glu Gly Glu Gly Val Leu Ser Lys Ala Asp Leu 3110 3115 3120 Glu Asn Pro His Pro Leu Glu Lys Lys Ile Thr Gln Trp Leu Glu 3125 3130 3135 Thr Lys Gly Val Glu Arg Leu Lys Arg Met Ala Ile Ser Gly Asp 3140 3145 3150 Asp Cys Val Val Lys Pro Ile Asp Asp Arg Phe Ala Asn Ala Leu 3155 3160 3165 Leu Ala Leu Asn Asp Met Gly Lys Val Arg Lys Asp Ile Pro Gln 3170 3175 3180 Trp Gln Pro Ser Lys Gly Trp His Asp Trp Gln Gln Val Pro Phe 3185 3190 3195 Cys Ser His His Phe His Glu Leu Ile Met Lys Asp Gly Arg Lys 3200 3205 3210 Leu Val Val Pro Cys Arg Pro Gln Asp Glu Leu Ile Gly Arg Ala 3215 3220 3225 Arg Ile Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala Cys 3230 3235 3240 Leu Gly Lys Ala Tyr Ala Gln Met Trp Ala Leu Met Tyr Phe His 3245 3250 3255 Arg Arg Asp Leu Arg Leu Ala Ser Asn Ala Ile Cys Ser Ala Val 3260 3265 3270 Pro Val His Trp Val Pro Thr Ser Arg Thr Thr Trp Ser Ile His 3275 3280 3285 Ala His His Gln Trp Met Thr Thr Glu Asp Met Leu Thr Val Trp 3290 3295 3300 Asn Arg Val Trp Ile Glu Asp Asn Pro Trp Met Glu Asp Lys Thr 3305 3310 3315 Pro Val Thr Thr Trp Glu Asp Val Pro Tyr Leu Gly Lys Arg Glu 3320 3325 3330 Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ser Arg Ala Thr 3335 3340 3345 Trp Ala Gln Asn Ile Leu Thr Ala Ile Gln Gln Val Arg Ser Leu 3350 3355 3360 Ile Gly Asn Glu Glu Phe Leu Asp Tyr Met Pro Ser Met Lys Arg 3365 3370 3375 Phe Arg Lys Glu Glu Glu Ser Glu Gly Ala Ile Trp 3380 3385 3390 <210> 12 <211> 3387 <212> PRT <213> Dengue virus type 4 <400> 12 Met Asn Gln Arg Lys Lys Val Val Arg Pro Pro Phe Asn Met Leu Lys 1 5 10 15 Arg Glu Arg Asn Arg Val Ser Thr Pro Gln Gly Leu Val Lys Arg Phe 20 25 30 Ser Thr Gly Leu Phe Ser Gly Lys Gly Pro Leu Arg Met Val Leu Ala 35 40 45 Phe Ile Thr Phe Leu Arg Val Leu Ser Ile Pro Pro Thr Ala Gly Ile 50 55 60 Leu Lys Arg Trp Gly Gln Leu Lys Lys Asn Lys Ala Ile Arg Ile Leu 65 70 75 80 Ile Gly Phe Arg Lys Glu Ile Gly Arg Met Leu Asn Ile Leu Asn Gly 85 90 95 Arg Lys Arg Ser Thr Ile Thr Leu Leu Cys Leu Ile Pro Thr Val Met 100 105 110 Ala Phe His Leu Ser Thr Arg Asp Gly Glu Pro Leu Met Ile Val Ala 115 120 125 Lys His Glu Arg Gly Arg Pro Leu Leu Phe Lys Thr Thr Glu Gly Ile 130 135 140 Asn Lys Cys Thr Leu Ile Ala Met Asp Leu Gly Glu Met Cys Glu Asp 145 150 155 160 Thr Val Thr Tyr Lys Cys Pro Leu Leu Val Asn Thr Glu Pro Glu Asp 165 170 175 Ile Asp Cys Trp Cys Asn Leu Thr Ser Thr Trp Val Met Tyr Gly Thr 180 185 190 Cys Thr Gln Ser Gly Glu Arg Arg Arg Glu Lys Arg Ser Val Ala Leu 195 200 205 Thr Pro His Ser Gly Met Gly Leu Glu Thr Arg Ala Glu Thr Trp Met 210 215 220 Ser Ser Glu Gly Ala Trp Lys His Ala Gln Arg Val Glu Ser Trp Ile 225 230 235 240 Leu Arg Asn Pro Gly Phe Ala Leu Leu Ala Gly Phe Met Ala Tyr Met 245 250 255 Ile Gly Gln Thr Gly Ile Gln Arg Thr Val Phe Phe Val Leu Met Met 260 265 270 Leu Val Ala Pro Ser Tyr Gly Met Arg Cys Val Gly Val Gly Asn Arg 275 280 285 Asp Phe Val Glu Gly Val Ser Gly Gly Ala Trp Val Asp Leu Val Leu 290 295 300 Glu His Gly Gly Cys Val Thr Thr Met Ala Gln Gly Lys Pro Thr Leu 305 310 315 320 Asp Phe Glu Leu Thr Lys Thr Thr Ala Lys Glu Val Ala Leu Leu Arg 325 330 335 Thr Tyr Cys Ile Glu Ala Ser Ile Ser Asn Ile Thr Thr Ala Thr Arg 340 345 350 Cys Pro Thr Gln Gly Glu Pro Tyr Leu Lys Glu Glu Gln Asp Gln Gln 355 360 365 Tyr Ile Cys Arg Arg Asp Val Val Asp Arg Gly Trp Gly Asn Gly Cys 370 375 380 Gly Leu Phe Gly Lys Gly Gly Val Val Thr Cys Ala Lys Phe Ser Cys 385 390 395 400 Ser Gly Lys Ile Thr Gly Asn Leu Val Gln Ile Glu Asn Leu Glu Tyr 405 410 415 Thr Val Val Val Thr Val His Asn Gly Asp Thr His Ala Val Gly Asn 420 425 430 Asp Thr Ser Asn His Gly Val Thr Ala Thr Ile Thr Pro Arg Ser Pro 435 440 445 Ser Val Glu Val Lys Leu Pro Asp Tyr Gly Glu Leu Thr Leu Asp Cys 450 455 460 Glu Pro Arg Ser Gly Ile Asp Phe Asn Glu Met Ile Leu Met Lys Met 465 470 475 480 Lys Lys Lys Thr Trp Leu Val His Lys Gln Trp Phe Leu Asp Leu Pro 485 490 495 Leu Pro Trp Thr Ala Gly Ala Asp Thr Ser Glu Val His Trp Asn Tyr 500 505 510 Lys Glu Arg Met Val Thr Phe Lys Val Pro His Ala Lys Arg Gln Asp 515 520 525 Val Thr Val Leu Gly Ser Gln Glu Gly Ala Met His Ser Ala Leu Ala 530 535 540 Gly Ala Thr Glu Val Asp Ser Gly Asp Gly Asn His Met Phe Ala Gly 545 550 555 560 His Leu Lys Cys Lys Val Arg Met Glu Lys Leu Arg Ile Lys Gly Met 565 570 575 Ser Tyr Thr Met Cys Ser Gly Lys Phe Ser Ile Asp Lys Glu Met Ala 580 585 590 Glu Thr Gln His Gly Thr Thr Val Val Lys Val Lys Tyr Glu Gly Ala 595 600 605 Gly Ala Pro Cys Lys Val Pro Ile Glu Ile Arg Asp Val Asn Lys Glu 610 615 620 Lys Val Val Gly Arg Ile Ile Ser Ser Thr Pro Leu Ala Glu Asn Thr 625 630 635 640 Asn Ser Ala Thr Asn Ile Glu Leu Glu Pro Pro Phe Gly Asp Ser Tyr 645 650 655 Ile Val Ile Gly Val Gly Asn Ser Ala Leu Thr Leu His Trp Phe Arg 660 665 670 Lys Gly Ser Ser Ile Gly Lys Met Phe Glu Ser Thr Tyr Arg Gly Ala 675 680 685 Lys Arg Met Ala Ile Leu Gly Glu Thr Ala Trp Asp Phe Gly Ser Val 690 695 700 Gly Gly Leu Phe Thr Ser Leu Gly Lys Ala Val His Gln Val Phe Gly 705 710 715 720 Ser Val Tyr Thr Thr Met Phe Gly Gly Val Ser Trp Met Ile Arg Ile 725 730 735 Leu Ile Gly Phe Leu Val Leu Trp Ile Gly Thr Asn Ser Arg Asn Thr 740 745 750 Ser Met Ala Met Thr Cys Ile Ala Val Gly Gly Ile Thr Leu Phe Leu 755 760 765 Gly Phe Thr Val Gln Ala Asp Met Gly Cys Val Val Ser Trp Ser Gly 770 775 780 Lys Glu Leu Lys Cys Gly Ser Gly Ile Phe Val Val Asp Asn Val His 785 790 795 800 Thr Trp Thr Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ala Arg Leu 805 810 815 Ala Ser Ala Ile Leu Asn Ala His Lys Asp Gly Val Cys Gly Ile Arg 820 825 830 Ser Thr Thr Arg Leu Glu Asn Val Met Trp Lys Gln Ile Thr Asn Glu 835 840 845 Leu Asn Tyr Val Leu Trp Glu Gly Gly His Asp Leu Thr Val Val Ala 850 855 860 Gly Asp Val Lys Gly Val Leu Thr Lys Gly Lys Arg Ala Leu Thr Pro 865 870 875 880 Pro Val Asn Asp Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala Lys 885 890 895 Ile Phe Thr Pro Glu Ala Arg Asn Ser Thr Phe Leu Ile Asp Gly Pro 900 905 910 Asp Thr Ser Glu Cys Pro Asn Glu Arg Arg Ala Trp Asn Phe Leu Glu 915 920 925 Val Glu Asp Tyr Gly Phe Gly Met Phe Thr Thr Asn Ile Trp Met Lys 930 935 940 Phe Arg Glu Gly Ser Ser Glu Val Cys Asp His Arg Leu Met Ser Ala 945 950 955 960 Ala Ile Lys Asp Gln Lys Ala Val His Ala Asp Met Gly Tyr Trp Ile 965 970 975 Glu Ser Ser Lys Asn Gln Thr Trp Gln Ile Glu Lys Ala Ser Leu Ile 980 985 990 Glu Val Lys Thr Cys Leu Trp Pro Lys Thr His Thr Leu Trp Ser Asn 995 1000 1005 Gly Val Leu Glu Ser Gln Met Leu Ile Pro Lys Ser Tyr Ala Gly 1010 1015 1020 Pro Phe Ser His His Asn Tyr Arg Gln Gly Tyr Ala Thr Gln Thr 1025 1030 1035 Val Gly Pro Trp His Leu Gly Lys Leu Glu Ile Asp Phe Gly Glu 1040 1045 1050 Cys Pro Gly Thr Thr Val Ala Ile Gln Glu Asp Cys Asp His Arg 1055 1060 1065 Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys Leu Val Thr 1070 1075 1080 Gln Trp Cys Cys Arg Ser Cys Thr Met Pro Pro Leu Arg Phe Leu 1085 1090 1095 Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu Ser 1100 1105 1110 Glu Lys Glu Glu Asn Met Val Lys Ser Gln Val Thr Ala Gly Gln 1115 1120 1125 Gly Thr Ser Glu Thr Phe Ser Met Gly Leu Leu Cys Leu Thr Leu 1130 1135 1140 Phe Val Glu Glu Cys Leu Arg Arg Arg Val Thr Arg Lys His Met 1145 1150 1155 Ile Leu Val Val Val Ile Thr Leu Cys Ala Ile Ile Leu Gly Gly 1160 1165 1170 Leu Thr Trp Met Asp Leu Leu Arg Ala Leu Ile Met Leu Gly Asp 1175 1180 1185 Thr Met Ser Gly Arg Ile Gly Gly Gln Ile His Leu Ala Ile Met 1190 1195 1200 Ala Val Phe Lys Met Ser Pro Gly Tyr Val Leu Gly Val Phe Leu 1205 1210 1215 Arg Lys Leu Thr Ser Arg Glu Thr Ala Leu Met Val Ile Gly Met 1220 1225 1230 Ala Met Thr Thr Val Leu Ser Ile Pro His Asp Leu Met Glu Leu 1235 1240 1245 Ile Asp Gly Ile Ser Leu Gly Leu Ile Leu Leu Lys Ile Val Thr 1250 1255 1260 His Phe Asp Asn Thr Gln Val Gly Thr Leu Ala Leu Ser Leu Thr 1265 1270 1275 Phe Ile Arg Ser Thr Met Pro Leu Val Met Ala Trp Arg Thr Ile 1280 1285 1290 Met Ala Val Leu Phe Val Val Thr Leu Ile Pro Leu Cys Arg Thr 1295 1300 1305 Ser Cys Leu Gln Lys Gln Ser His Trp Val Glu Ile Thr Ala Leu 1310 1315 1320 Ile Leu Gly Ala Gln Ala Leu Pro Val Tyr Leu Met Thr Leu Met 1325 1330 1335 Lys Gly Ala Ser Arg Arg Ser Trp Pro Leu Asn Glu Gly Ile Met 1340 1345 1350 Ala Val Gly Leu Val Ser Leu Leu Gly Ser Ala Leu Leu Lys Asn 1355 1360 1365 Asp Val Pro Leu Ala Gly Pro Met Val Ala Gly Gly Leu Leu Leu 1370 1375 1380 Ala Ala Tyr Val Met Ser Gly Ser Ser Ala Asp Leu Ser Leu Glu 1385 1390 1395 Lys Ala Ala Asn Val Gln Trp Asp Glu Met Ala Asp Ile Thr Gly 1400 1405 1410 Ser Ser Pro Ile Ile Glu Val Lys Gln Asp Glu Asp Gly Ser Phe 1415 1420 1425 Ser Ile Arg Asp Val Glu Glu Thr Asn Met Ile Thr Leu Leu Val 1430 1435 1440 Lys Leu Ala Leu Ile Thr Val Ser Gly Leu Tyr Pro Leu Ala Ile 1445 1450 1455 Pro Val Thr Met Thr Leu Trp Tyr Met Trp Gln Val Lys Thr Gln 1460 1465 1470 Arg Ser Gly Ala Leu Trp Asp Val Pro Ser Pro Ala Ala Thr Gln 1475 1480 1485 Lys Ala Ala Leu Ser Glu Gly Val Tyr Arg Ile Met Gln Arg Gly 1490 1495 1500 Leu Phe Gly Lys Thr Gln Val Gly Val Gly Ile His Met Glu Gly 1505 1510 1515 Val Phe His Thr Met Trp His Val Thr Arg Gly Ser Val Ile Cys 1520 1525 1530 His Glu Thr Gly Arg Leu Glu Pro Ser Trp Ala Asp Val Arg Asn 1535 1540 1545 Asp Met Ile Ser Tyr Gly Gly Gly Trp Arg Leu Gly Asp Lys Trp 1550 1555 1560 Asp Lys Glu Glu Asp Val Gln Val Leu Ala Ile Glu Pro Gly Lys 1565 1570 1575 Asn Pro Lys His Val Gln Thr Lys Pro Gly Leu Phe Lys Thr Leu 1580 1585 1590 Thr Gly Glu Ile Gly Ala Val Thr Leu Asp Phe Lys Pro Gly Thr 1595 1600 1605 Ser Gly Ser Pro Ile Ile Asn Arg Lys Gly Lys Val Ile Gly Leu 1610 1615 1620 Tyr Gly Asn Gly Val Val Thr Lys Ser Gly Asp Tyr Val Ser Ala 1625 1630 1635 Ile Thr Gln Ala Glu Arg Ile Gly Glu Pro Asp Tyr Glu Val Asp 1640 1645 1650 Glu Asp Ile Phe Arg Lys Lys Arg Leu Thr Ile Met Asp Leu His 1655 1660 1665 Pro Gly Ala Gly Lys Thr Lys Arg Ile Leu Pro Ser Ile Val Arg 1670 1675 1680 Glu Ala Leu Lys Arg Arg Leu Arg Thr Leu Ile Leu Ala Pro Thr 1685 1690 1695 Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu Pro 1700 1705 1710 Ile Arg Tyr Gln Thr Pro Ala Val Lys Ser Glu His Thr Gly Arg 1715 1720 1725 Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Thr Arg Leu 1730 1735 1740 Leu Ser Ser Thr Arg Val Pro Asn Tyr Asn Leu Ile Val Met Asp 1745 1750 1755 Glu Ala His Phe Thr Asp Pro Ser Ser Val Ala Ala Arg Gly Tyr 1760 1765 1770 Ile Ser Thr Arg Val Glu Met Gly Glu Ala Ala Ala Ile Phe Met 1775 1780 1785 Thr Ala Thr Pro Pro Gly Ala Thr Asp Pro Phe Pro Gln Ser Asn 1790 1795 1800 Ser Pro Ile Glu Asp Ile Glu Arg Glu Ile Pro Glu Arg Ser Trp 1805 1810 1815 Asn Thr Gly Phe Asp Trp Ile Thr Asp Tyr Gln Gly Lys Thr Val 1820 1825 1830 Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Asn Cys 1835 1840 1845 Leu Arg Lys Ser Gly Lys Lys Val Ile Gln Leu Ser Arg Lys Thr 1850 1855 1860 Phe Asp Thr Glu Tyr Pro Lys Thr Lys Leu Thr Asp Trp Asp Phe 1865 1870 1875 Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Arg Ala 1880 1885 1890 Gly Arg Val Ile Asp Pro Arg Arg Cys Leu Lys Pro Val Ile Leu 1895 1900 1905 Thr Asp Gly Pro Glu Arg Val Ile Leu Ala Gly Pro Ile Pro Val 1910 1915 1920 Thr Pro Ala Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg Asn 1925 1930 1935 Pro Ala Gln Glu Asp Asp Gln Tyr Val Phe Ser Gly Asp Pro Leu 1940 1945 1950 Lys Asn Asp Glu Asp His Ala His Trp Thr Glu Ala Lys Met Leu 1955 1960 1965 Leu Asp Asn Ile Tyr Thr Pro Glu Gly Ile Ile Pro Thr Leu Phe 1970 1975 1980 Gly Pro Glu Arg Glu Lys Thr Gln Ala Ile Asp Gly Glu Phe Arg 1985 1990 1995 Leu Arg Gly Glu Gln Arg Lys Thr Phe Val Glu Leu Met Arg Arg 2000 2005 2010 Gly Asp Leu Pro Val Trp Leu Ser Tyr Lys Val Ala Ser Ala Gly 2015 2020 2025 Ile Ser Tyr Lys Asp Arg Glu Trp Cys Phe Thr Gly Glu Arg Asn 2030 2035 2040 Asn Gln Ile Leu Glu Glu Asn Met Glu Val Glu Ile Trp Thr Arg 2045 2050 2055 Glu Gly Glu Lys Lys Lys Leu Arg Pro Arg Trp Leu Asp Ala Arg 2060 2065 2070 Val Tyr Ala Asp Pro Met Ala Leu Lys Asp Phe Lys Glu Phe Ala 2075 2080 2085 Ser Gly Arg Lys Ser Ile Thr Leu Asp Ile Leu Thr Glu Ile Ala 2090 2095 2100 Ser Leu Pro Thr Tyr Leu Ser Ser Arg Ala Lys Leu Ala Leu Asp 2105 2110 2115 Asn Ile Val Met Leu His Thr Thr Glu Arg Gly Gly Arg Ala Tyr 2120 2125 2130 Gln His Ala Leu Asn Glu Leu Pro Glu Ser Leu Glu Thr Leu Met 2135 2140 2145 Leu Val Ala Leu Leu Gly Ala Met Thr Ala Gly Ile Phe Leu Phe 2150 2155 2160 Phe Met Gln Gly Lys Gly Ile Gly Lys Leu Ser Met Gly Leu Ile 2165 2170 2175 Thr Ile Ala Val Ala Ser Gly Leu Phe Trp Val Ala Glu Ile Gln 2180 2185 2190 Pro Gln Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe Leu Met 2195 2200 2205 Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln Asp 2210 2215 2220 Asn Gln Leu Ile Tyr Val Ile Leu Thr Ile Leu Thr Ile Ile Gly 2225 2230 2235 Leu Ile Ala Ala Asn Glu Met Gly Leu Ile Glu Lys Thr Lys Thr 2240 2245 2250 Asp Phe Gly Phe Tyr Gln Val Lys Thr Glu Thr Thr Ile Leu Asp 2255 2260 2265 Val Asp Leu Arg Pro Ala Ser Ala Trp Thr Leu Tyr Ala Val Ala 2270 2275 2280 Thr Thr Ile Leu Thr Pro Met Leu Arg His Thr Ile Glu Asn Thr 2285 2290 2295 Ser Ala Asn Leu Ser Leu Ala Ala Ile Ala Asn Gln Ala Ala Val 2300 2305 2310 Leu Met Gly Leu Gly Lys Gly Trp Pro Leu His Arg Met Asp Leu 2315 2320 2325 Gly Val Pro Leu Leu Ala Met Gly Cys Tyr Ser Gln Val Asn Pro 2330 2335 2340 Thr Thr Leu Thr Ala Ser Leu Val Met Leu Ser Val His Tyr Ala 2345 2350 2355 Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr Arg Glu Ala Gln 2360 2365 2370 Lys Arg Thr Ala Ala Gly Ile Met Lys Asn Pro Thr Val Asp Gly 2375 2380 2385 Ile Thr Val Ile Asp Leu Glu Pro Ile Ser Tyr Asp Pro Lys Phe 2390 2395 2400 Glu Lys Gln Leu Gly Gln Val Met Leu Leu Val Leu Cys Ala Gly 2405 2410 2415 Gln Leu Leu Leu Met Arg Thr Thr Trp Ala Phe Cys Glu Val Leu 2420 2425 2430 Thr Leu Ala Thr Gly Pro Ile Leu Thr Leu Trp Glu Gly Asn Pro 2435 2440 2445 Gly Arg Phe Trp Asn Thr Thr Ile Ala Val Ser Thr Ala Asn Ile 2450 2455 2460 Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu Ala Phe Ser Leu 2465 2470 2475 Ile Lys Asn Val Gln Thr Pro Arg Arg Gly Thr Gly Thr Thr Gly 2480 2485 2490 Glu Thr Leu Gly Glu Lys Trp Lys Arg Gln Leu Asn Ser Leu Asp 2495 2500 2505 Arg Lys Glu Phe Glu Glu Tyr Lys Arg Ser Gly Ile Leu Glu Val 2510 2515 2520 Asp Arg Thr Glu Ala Lys Ser Ala Leu Lys Asp Gly Ser Lys Ile 2525 2530 2535 Lys His Ala Val Ser Arg Gly Ser Ser Lys Ile Arg Trp Ile Val 2540 2545 2550 Glu Arg Gly Met Val Lys Pro Lys Gly Lys Val Val Asp Leu Gly 2555 2560 2565 Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Met Ala Thr Leu Lys Asn 2570 2575 2580 Val Thr Glu Val Lys Gly Tyr Thr Lys Gly Gly Pro Gly His Glu 2585 2590 2595 Glu Pro Ile Pro Met Ala Thr Tyr Gly Trp Asn Leu Val Lys Leu 2600 2605 2610 His Ser Gly Val Asp Val Phe Tyr Lys Pro Thr Glu Gln Val Asp 2615 2620 2625 Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Ser Asn Pro Thr Ile 2630 2635 2640 Glu Glu Gly Arg Thr Leu Arg Val Leu Lys Met Val Glu Pro Trp 2645 2650 2655 Leu Ser Ser Lys Pro Glu Phe Cys Ile Lys Val Leu Asn Pro Tyr 2660 2665 2670 Met Pro Thr Val Ile Glu Glu Leu Glu Lys Leu Gln Arg Lys His 2675 2680 2685 Gly Gly Asn Leu Val Arg Cys Pro Leu Ser Arg Asn Ser Thr His 2690 2695 2700 Glu Met Tyr Trp Val Ser Gly Ala Ser Gly Asn Ile Val Ser Ser 2705 2710 2715 Val Asn Thr Thr Ser Lys Met Leu Leu Asn Arg Phe Thr Thr Arg 2720 2725 2730 His Arg Lys Pro Thr Tyr Glu Lys Asp Val Asp Leu Gly Ala Gly 2735 2740 2745 Thr Arg Ser Val Ser Thr Glu Thr Glu Lys Pro Asp Met Thr Ile 2750 2755 2760 Ile Gly Arg Arg Leu Gln Arg Leu Gln Glu Glu His Lys Glu Thr 2765 2770 2775 Trp His Tyr Asp Gln Glu Asn Pro Tyr Arg Thr Trp Ala Tyr His 2780 2785 2790 Gly Ser Tyr Glu Ala Pro Ser Thr Gly Ser Ala Ser Ser Met Val 2795 2800 2805 Asn Gly Val Val Lys Leu Leu Thr Lys Pro Trp Asp Val Val Pro 2810 2815 2820 Met Val Thr Gln Leu Ala Met Thr Asp Thr Thr Pro Phe Gly Gln 2825 2830 2835 Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg Thr Pro Gln Pro 2840 2845 2850 Lys Pro Gly Thr Arg Met Val Met Thr Thr Thr Ala Asn Trp Leu 2855 2860 2865 Trp Ala Leu Leu Gly Lys Lys Lys Asn Pro Arg Leu Cys Thr Arg 2870 2875 2880 Glu Glu Phe Ile Ser Lys Val Arg Ser Asn Ala Ala Ile Gly Ala 2885 2890 2895 Val Phe Gln Glu Glu Gln Gly Trp Thr Ser Ala Ser Glu Ala Val 2900 2905 2910 Asn Asp Ser Arg Phe Trp Glu Leu Val Asp Lys Glu Arg Ala Leu 2915 2920 2925 His Gln Glu Gly Lys Cys Glu Ser Cys Val Tyr Asn Met Met Gly 2930 2935 2940 Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Arg Ala Lys Gly Ser 2945 2950 2955 Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg Phe Leu Glu Phe 2960 2965 2970 Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp Phe Gly Arg Glu 2975 2980 2985 Asn Ser Trp Ser Gly Val Glu Gly Glu Gly Leu His Arg Leu Gly 2990 2995 3000 Tyr Ile Leu Glu Glu Ile Asp Lys Lys Asp Gly Asp Leu Met Tyr 3005 3010 3015 Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile Thr Glu Asp Asp 3020 3025 3030 Leu Gln Asn Glu Glu Leu Ile Thr Glu Gln Met Ala Pro His His 3035 3040 3045 Lys Ile Leu Ala Lys Ala Ile Phe Lys Leu Thr Tyr Gln Asn Lys 3050 3055 3060 Val Val Lys Val Leu Arg Pro Thr Pro Arg Gly Ala Val Met Asp 3065 3070 3075 Ile Ile Ser Arg Lys Asp Gln Arg Gly Ser Gly Gln Val Gly Thr 3080 3085 3090 Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Val Gln Leu Ile Arg 3095 3100 3105 Gln Met Glu Ala Glu Gly Val Ile Thr Gln Asp Asp Met Gln Asn 3110 3115 3120 Pro Lys Gly Leu Lys Glu Arg Val Glu Lys Trp Leu Lys Glu Cys 3125 3130 3135 Gly Val Asp Arg Leu Lys Arg Met Ala Ile Ser Gly Asp Asp Cys 3140 3145 3150 Val Val Lys Pro Leu Asp Glu Arg Phe Gly Thr Ser Leu Leu Phe 3155 3160 3165 Leu Asn Asp Met Gly Lys Val Arg Lys Asp Ile Pro Gln Trp Glu 3170 3175 3180 Pro Ser Lys Gly Trp Lys Asn Trp Gln Glu Val Pro Phe Cys Ser 3185 3190 3195 His His Phe His Lys Ile Phe Met Lys Asp Gly Arg Ser Leu Val 3200 3205 3210 Val Pro Cys Arg Asn Gln Asp Glu Leu Ile Gly Arg Ala Arg Ile 3215 3220 3225 Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala Cys Leu Gly 3230 3235 3240 Lys Ala Tyr Ala Gln Met Trp Ser Leu Met Tyr Phe His Arg Arg 3245 3250 3255 Asp Leu Arg Leu Ala Ser Met Ala Ile Cys Ser Ala Val Pro Thr 3260 3265 3270 Glu Trp Phe Pro Thr Ser Arg Thr Thr Trp Ser Ile His Ala His 3275 3280 3285 His Gln Trp Met Thr Thr Glu Asp Met Leu Lys Val Trp Asn Arg 3290 3295 3300 Val Trp Ile Glu Asp Asn Pro Asn Met Thr Asp Lys Thr Pro Val 3305 3310 3315 His Ser Trp Glu Asp Ile Pro Tyr Leu Gly Lys Arg Glu Asp Leu 3320 3325 3330 Trp Cys Gly Ser Leu Ile Gly Leu Ser Ser Arg Ala Thr Trp Ala 3335 3340 3345 Lys Asn Ile His Thr Ala Ile Thr Gln Val Arg Asn Leu Ile Gly 3350 3355 3360 Lys Glu Glu Tyr Val Asp Tyr Met Pro Val Met Lys Arg Tyr Ser 3365 3370 3375 Ala Pro Ser Glu Ser Glu Gly Val Leu 3380 3385 <210> 13 <211> 3392 <212> PRT <213> Dengue virus type 1 <220> <221> VARIANT <222> (482)..(482) <223> Xaa stands for Lys or Glu <220> <221> VARIANT <222> (484)..(484) <223> Xaa stands for Lys or Arg <220> <221> VARIANT <222> (2364)..(2364) <223> Xaa stands for Thr or Ala <400> 13 Met Asn Asn Gln Arg Lys Lys Thr Ala Arg Pro Ser Phe Asn Met Leu 1 5 10 15 Lys Arg Ala Arg Asn Arg Val Ser Thr Val Ser Gln Leu Ala Lys Arg 20 25 30 Phe Ser Lys Gly Leu Leu Ser Gly Gln Gly Pro Met Lys Leu Val Met 35 40 45 Ala Phe Ile Ala Phe Leu Arg Phe Leu Ala Ile Pro Pro Thr Ala Gly 50 55 60 Ile Leu Ala Arg Trp Gly Ser Phe Lys Lys Asn Gly Ala Ile Lys Val 65 70 75 80 Leu Arg Gly Phe Lys Lys Glu Ile Ser Asn Met Leu Asn Ile Met Asn 85 90 95 Arg Arg Lys Arg Ser Val Thr Met Leu Leu Met Leu Met Pro Thr Ala 100 105 110 Leu Ala Phe His Leu Thr Thr Arg Gly Gly Glu Pro His Met Ile Val 115 120 125 Ser Lys Gln Glu Arg Gly Lys Ser Leu Leu Phe Lys Thr Ser Ala Gly 130 135 140 Val Asn Met Cys Thr Leu Ile Ala Met Asp Leu Gly Glu Leu Cys Glu 145 150 155 160 Asp Thr Met Thr Tyr Lys Cys Pro Arg Ile Thr Glu Ala Glu Pro Asp 165 170 175 Asp Val Asp Cys Trp Cys Asn Ala Thr Asp Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Ser Gln Thr Gly Glu His Arg Arg Asp Lys Arg Ser Val Ala 195 200 205 Leu Ala Pro His Val Gly Leu Gly Leu Glu Thr Arg Thr Glu Thr Trp 210 215 220 Met Ser Ser Glu Gly Ala Trp Lys Gln Ile Gln Arg Val Glu Thr Trp 225 230 235 240 Ala Leu Arg His Pro Gly Phe Thr Val Ile Ala Leu Phe Leu Ala His 245 250 255 Ala Ile Gly Thr Ser Ile Thr Gln Lys Gly Ile Ile Phe Ile Leu Leu 260 265 270 Met Leu Val Thr Pro Ser Met Ala Met Arg Cys Val Gly Ile Gly Ser 275 280 285 Arg Asp Phe Val Glu Gly Leu Ser Gly Ala Thr Trp Val Asp Val Val 290 295 300 Leu Glu His Gly Ser Cys Val Thr Thr Met Ala Lys Asp Lys Pro Thr 305 310 315 320 Leu Asp Ile Glu Leu Leu Lys Thr Glu Val Thr Asn Pro Ala Val Leu 325 330 335 Arg Lys Leu Cys Ile Glu Ala Lys Ile Ser Asn Thr Thr Thr Asp Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Ala Thr Leu Val Glu Glu Gln Asp Ala 355 360 365 Asn Phe Val Cys Arg Arg Thr Phe Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Ser Leu Leu Thr Cys Ala Lys Phe Lys 385 390 395 400 Cys Val Thr Lys Leu Glu Gly Lys Ile Val Gln Tyr Glu Asn Leu Lys 405 410 415 Tyr Ser Val Ile Val Thr Val His Thr Gly Asp Gln His Gln Val Gly 420 425 430 Asn Glu Ser Thr Glu His Gly Thr Thr Ala Thr Ile Thr Pro Gln Ala 435 440 445 Pro Thr Thr Glu Ile Gln Leu Thr Asp Tyr Gly Ala Leu Thr Leu Asp 450 455 460 Cys Ser Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Val Leu Leu Thr 465 470 475 480 Met Xaa Lys Xaa Ser Trp Leu Val His Lys Gln Trp Phe Leu Asp Leu 485 490 495 Pro Leu Pro Trp Thr Ser Gly Ala Ser Thr Ser Gln Glu Thr Trp Asn 500 505 510 Arg Gln Asp Leu Leu Val Thr Phe Lys Thr Ala His Ala Lys Lys Gln 515 520 525 Glu Val Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu 530 535 540 Thr Gly Ala Thr Glu Ile Gln Thr Ser Gly Thr Thr Thr Ile Phe Ala 545 550 555 560 Gly His Leu Lys Cys Arg Leu Arg Met Asp Lys Leu Thr Leu Lys Gly 565 570 575 Met Ser Tyr Val Met Cys Thr Gly Ser Phe Lys Leu Glu Lys Glu Val 580 585 590 Ala Glu Thr Gln His Gly Thr Val Leu Val Gln Ile Lys Tyr Glu Gly 595 600 605 Thr Asp Ala Pro Cys Lys Ile Pro Phe Ser Thr Gln Asp Glu Lys Gly 610 615 620 Val Thr Gln Asn Gly Arg Leu Ile Thr Ala Asn Pro Ile Val Thr Asp 625 630 635 640 Lys Glu Lys Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser 645 650 655 Tyr Ile Val Ile Gly Ala Gly Glu Lys Ala Leu Lys Leu Ser Trp Phe 660 665 670 Lys Lys Gly Ser Ser Ile Gly Lys Met Phe Glu Ala Thr Ala Arg Gly 675 680 685 Ala Arg Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 690 695 700 Ile Gly Gly Val Phe Thr Ser Val Gly Lys Leu Val His Gln Ile Phe 705 710 715 720 Gly Thr Ala Tyr Gly Val Leu Phe Ser Gly Val Ser Trp Thr Met Lys 725 730 735 Ile Gly Ile Gly Val Leu Leu Thr Trp Leu Gly Leu Asn Ser Arg Asn 740 745 750 Thr Ser Leu Ser Met Thr Cys Ile Ala Val Gly Leu Val Thr Leu Tyr 755 760 765 Leu Gly Val Met Val Gln Ala Asp Ser Gly Cys Val Ile Asn Trp Lys 770 775 780 Gly Arg Glu Leu Lys Cys Gly Ser Gly Ile Phe Val Thr Asn Glu Val 785 790 795 800 His Thr Trp Thr Glu Gln Tyr Lys Phe Gln Ala Asp Ser Pro Lys Arg 805 810 815 Leu Ser Ala Ala Ile Gly Lys Ala Trp Glu Glu Gly Val Cys Gly Ile 820 825 830 Arg Ser Ala Thr Arg Leu Glu Asn Ile Met Trp Lys Gln Ile Ser Asn 835 840 845 Glu Leu Asn His Ile Leu Leu Glu Asn Asp Met Lys Phe Thr Val Val 850 855 860 Val Gly Asp Val Ala Gly Ile Leu Ala Gln Gly Lys Lys Met Ile Arg 865 870 875 880 Pro Gln Pro Met Glu His Lys Tyr Ser Trp Lys Ser Trp Gly Lys Ala 885 890 895 Lys Ile Ile Gly Ala Asp Val Gln Asn Ser Thr Phe Ile Ile Asp Gly 900 905 910 Pro Asn Thr Pro Glu Cys Pro Asp Asp Gln Arg Ala Trp Asn Ile Trp 915 920 925 Glu Val Glu Asp Tyr Gly Phe Gly Ile Phe Thr Thr Asn Ile Trp Leu 930 935 940 Lys Leu Arg Asp Ser Tyr Thr Gln Leu Cys Asp His Arg Leu Met Ser 945 950 955 960 Ala Ala Ile Lys Asp Ser Lys Ala Val His Ala Asp Met Gly Tyr Trp 965 970 975 Ile Glu Ser Glu Lys Asn Glu Thr Trp Lys Leu Ala Arg Ala Ser Phe 980 985 990 Ile Glu Val Lys Thr Cys Ile Trp Pro Lys Ser His Thr Leu Trp Ser 995 1000 1005 Asn Gly Val Leu Glu Ser Glu Met Ile Ile Pro Lys Ile Tyr Gly 1010 1015 1020 Gly Pro Ile Ser Gln His Asn Tyr Arg Pro Gly Tyr Phe Thr Gln 1025 1030 1035 Thr Val Gly Pro Trp His Leu Gly Lys Leu Glu Leu Asp Phe Asp 1040 1045 1050 Leu Cys Glu Gly Thr Thr Val Val Val Asp Glu His Cys Gly Asn 1055 1060 1065 Arg Gly Pro Ser Leu Arg Thr Thr Thr Val Thr Gly Lys Ile Ile 1070 1075 1080 His Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Phe 1085 1090 1095 Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Val 1100 1105 1110 Lys Glu Lys Glu Glu Asn Leu Val Lys Ser Met Val Ser Ala Gly 1115 1120 1125 Ser Gly Glu Val Asp Ser Phe Ser Leu Gly Leu Leu Cys Ile Ser 1130 1135 1140 Ile Met Ile Glu Glu Val Met Arg Ser Arg Trp Ser Arg Lys Met 1145 1150 1155 Leu Met Thr Gly Thr Leu Ala Val Phe Leu Leu Leu Ile Met Gly 1160 1165 1170 Gln Leu Thr Trp Asn Asp Leu Ile Arg Leu Cys Ile Met Val Gly 1175 1180 1185 Ala Asn Ala Ser Asp Arg Met Gly Met Gly Thr Thr Tyr Leu Ala 1190 1195 1200 Leu Met Ala Thr Phe Lys Met Arg Pro Met Phe Ala Val Gly Leu 1205 1210 1215 Leu Phe Arg Arg Leu Thr Ser Arg Glu Val Leu Leu Leu Thr Ile 1220 1225 1230 Gly Leu Ser Leu Val Ala Ser Val Glu Leu Pro Asn Ser Leu Glu 1235 1240 1245 Glu Leu Gly Asp Gly Leu Ala Met Gly Ile Met Ile Leu Lys Leu 1250 1255 1260 Leu Thr Asp Phe Gln Ser His Gln Leu Trp Ala Thr Leu Leu Ser 1265 1270 1275 Leu Thr Phe Ile Lys Thr Thr Phe Ser Leu His Tyr Ala Trp Lys 1280 1285 1290 Thr Met Ala Met Val Leu Ser Ile Val Ser Leu Phe Pro Leu Cys 1295 1300 1305 Leu Ser Thr Thr Ser Gln Lys Thr Thr Trp Leu Pro Val Leu Leu 1310 1315 1320 Gly Ser Leu Gly Cys Lys Pro Leu Thr Met Phe Leu Ile Ala Glu 1325 1330 1335 Asn Lys Ile Trp Gly Arg Lys Ser Trp Pro Leu Asn Glu Gly Ile 1340 1345 1350 Met Ala Val Gly Ile Val Ser Ile Leu Leu Ser Ser Leu Leu Lys 1355 1360 1365 Asn Asp Val Pro Leu Ala Gly Pro Leu Ile Ala Gly Gly Met Leu 1370 1375 1380 Ile Ala Cys Tyr Val Ile Ser Gly Ser Ser Ala Asp Leu Ser Leu 1385 1390 1395 Glu Lys Ala Ala Glu Val Ser Trp Glu Glu Glu Ala Glu His Ser 1400 1405 1410 Gly Ala Ser His Asn Ile Leu Val Glu Val Gln Asp Asp Gly Thr 1415 1420 1425 Met Lys Ile Lys Asp Glu Glu Arg Asp Asp Thr Leu Thr Ile Leu 1430 1435 1440 Leu Lys Ala Thr Leu Leu Ala Val Ser Gly Val Tyr Pro Leu Ser 1445 1450 1455 Ile Pro Ala Thr Leu Phe Val Trp Tyr Phe Trp Gln Lys Lys Lys 1460 1465 1470 Gln Arg Ser Gly Val Leu Trp Asp Thr Pro Ser Pro Pro Glu Val 1475 1480 1485 Glu Arg Ala Val Leu Asp Asp Gly Ile Tyr Arg Ile Met Gln Arg 1490 1495 1500 Gly Leu Leu Gly Arg Ser Gln Val Gly Val Gly Val Phe Gln Glu 1505 1510 1515 Asn Val Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu 1520 1525 1530 Met Tyr Gln Gly Lys Arg Leu Glu Pro Ser Trp Ala Ser Val Lys 1535 1540 1545 Lys Asp Leu Ile Ser Tyr Gly Gly Gly Trp Arg Leu Gln Gly Ser 1550 1555 1560 Trp Asn Thr Gly Glu Glu Val Gln Val Ile Ala Val Glu Pro Gly 1565 1570 1575 Lys Asn Pro Lys Asn Val Gln Thr Ala Pro Gly Thr Phe Lys Thr 1580 1585 1590 Pro Glu Gly Glu Val Gly Ala Ile Ala Leu Asp Phe Lys Pro Gly 1595 1600 1605 Thr Ser Gly Ser Pro Ile Val Asn Arg Glu Gly Lys Ile Val Gly 1610 1615 1620 Leu Tyr Gly Asn Gly Val Val Thr Thr Ser Gly Thr Tyr Val Ser 1625 1630 1635 Ala Ile Ala Gln Ala Lys Ala Ser Gln Glu Gly Pro Leu Pro Glu 1640 1645 1650 Ile Glu Asp Glu Val Phe Arg Lys Arg Lys Leu Thr Ile Met Asp 1655 1660 1665 Leu His Pro Gly Ser Gly Lys Thr Arg Arg Tyr Leu Pro Ala Ile 1670 1675 1680 Val Arg Glu Ala Ile Lys Arg Lys Leu Arg Thr Leu Ile Leu Ala 1685 1690 1695 Pro Thr Arg Val Val Ala Ser Glu Met Ala Glu Ala Leu Lys Gly 1700 1705 1710 Met Pro Ile Arg Tyr Gln Thr Thr Ala Val Lys Ser Glu His Thr 1715 1720 1725 Gly Lys Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met 1730 1735 1740 Arg Leu Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Met Ile Ile 1745 1750 1755 Met Asp Glu Ala His Phe Thr Asp Pro Ser Ser Ile Ala Ala Arg 1760 1765 1770 Gly Tyr Ile Ser Thr Arg Val Gly Met Gly Glu Ala Ala Ala Ile 1775 1780 1785 Phe Met Thr Ala Thr Pro Pro Gly Ser Val Glu Ala Phe Pro Gln 1790 1795 1800 Ser Asn Ala Val Ile Gln Asp Glu Glu Arg Asp Ile Pro Glu Arg 1805 1810 1815 Ser Trp Asn Ser Gly Tyr Glu Trp Ile Thr Asp Phe Pro Gly Lys 1820 1825 1830 Thr Val Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala 1835 1840 1845 Asn Cys Leu Arg Lys Asn Gly Lys Arg Val Ile Gln Leu Ser Arg 1850 1855 1860 Lys Thr Phe Asp Thr Glu Tyr Gln Lys Thr Lys Asn Asn Asp Trp 1865 1870 1875 Asp Tyr Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe 1880 1885 1890 Arg Ala Asp Arg Val Ile Asp Pro Arg Arg Cys Leu Lys Pro Val 1895 1900 1905 Ile Leu Lys Asp Gly Pro Glu Arg Val Ile Leu Ala Gly Pro Met 1910 1915 1920 Pro Val Thr Val Ala Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly 1925 1930 1935 Arg Asn His Asn Lys Glu Gly Asp Gln Tyr Ile Tyr Met Gly Gln 1940 1945 1950 Pro Leu Asn Asn Asp Glu Asp His Ala His Trp Thr Glu Ala Lys 1955 1960 1965 Met Leu Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ala 1970 1975 1980 Leu Phe Glu Pro Glu Arg Glu Lys Ser Ala Ala Ile Asp Gly Glu 1985 1990 1995 Tyr Arg Leu Arg Gly Glu Ala Arg Lys Thr Phe Val Glu Leu Met 2000 2005 2010 Arg Arg Gly Asp Leu Pro Val Trp Leu Ser Tyr Lys Val Ala Ser 2015 2020 2025 Glu Gly Phe Gln Tyr Ser Asp Arg Arg Trp Cys Phe Asp Gly Glu 2030 2035 2040 Arg Asn Asn Gln Val Leu Glu Glu Asn Met Asp Val Glu Ile Trp 2045 2050 2055 Thr Lys Glu Gly Glu Arg Lys Lys Leu Arg Pro Arg Trp Leu Asp 2060 2065 2070 Ala Arg Thr Tyr Ser Asp Pro Leu Ala Leu Arg Glu Phe Lys Glu 2075 2080 2085 Phe Ala Ala Gly Arg Arg Ser Val Ser Gly Asp Leu Ile Leu Glu 2090 2095 2100 Ile Gly Lys Leu Pro Gln His Leu Thr Gln Arg Ala Gln Asn Ala 2105 2110 2115 Leu Asp Asn Leu Val Met Leu His Asn Ser Glu Gln Gly Gly Arg 2120 2125 2130 Ala Tyr Arg His Ala Met Glu Glu Leu Pro Asp Thr Ile Glu Thr 2135 2140 2145 Leu Met Leu Leu Ala Leu Ile Ala Val Leu Thr Gly Gly Val Thr 2150 2155 2160 Leu Phe Phe Leu Ser Gly Lys Gly Leu Gly Lys Thr Ser Ile Gly 2165 2170 2175 Leu Leu Cys Val Met Ala Ser Ser Val Leu Leu Trp Met Ala Ser 2180 2185 2190 Val Glu Pro His Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe 2195 2200 2205 Leu Met Val Leu Leu Ile Pro Glu Pro Asp Arg Gln Arg Thr Pro 2210 2215 2220 Gln Asp Asn Gln Leu Ala Tyr Val Val Ile Gly Leu Leu Phe Met 2225 2230 2235 Ile Leu Thr Val Ala Ala Asn Glu Met Gly Leu Leu Glu Thr Thr 2240 2245 2250 Lys Lys Asp Leu Gly Ile Gly His Val Ala Val Glu Asn His His 2255 2260 2265 His Ala Ala Met Leu Asp Val Asp Leu His Pro Ala Ser Ala Trp 2270 2275 2280 Thr Leu Tyr Ala Val Ala Thr Thr Ile Ile Thr Pro Met Met Arg 2285 2290 2295 His Thr Ile Glu Asn Thr Thr Ala Asn Ile Ser Leu Thr Ala Ile 2300 2305 2310 Ala Asn Gln Ala Ala Ile Leu Met Gly Leu Asp Lys Gly Trp Pro 2315 2320 2325 Ile Ser Lys Met Asp Ile Gly Val Pro Leu Leu Ala Leu Gly Cys 2330 2335 2340 Tyr Ser Gln Val Asn Pro Leu Thr Leu Thr Ala Ala Val Leu Met 2345 2350 2355 Leu Val Ala His Tyr Xaa Ile Ile Gly Pro Gly Leu Gln Ala Lys 2360 2365 2370 Ala Thr Arg Glu Ala Gln Lys Arg Thr Ala Ala Gly Ile Met Lys 2375 2380 2385 Asn Pro Thr Ile Asp Gly Ile Val Ala Ile Asp Leu Asp Pro Val 2390 2395 2400 Val Tyr Asp Ala Lys Phe Glu Lys Gln Leu Gly Gln Ile Met Leu 2405 2410 2415 Leu Ile Leu Cys Thr Ser Gln Ile Leu Leu Met Arg Thr Thr Trp 2420 2425 2430 Ala Leu Cys Glu Ser Ile Thr Leu Ala Thr Gly Pro Leu Thr Thr 2435 2440 2445 Leu Trp Glu Gly Ser Pro Gly Lys Phe Trp Asn Thr Thr Ile Ala 2450 2455 2460 Val Ser Met Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala 2465 2470 2475 Gly Leu Ala Phe Ser Leu Met Lys Ser Leu Gly Gly Gly Arg Arg 2480 2485 2490 Gly Thr Gly Ala Gln Gly Glu Thr Leu Gly Glu Lys Trp Lys Arg 2495 2500 2505 Gln Leu Asn Gln Leu Ser Lys Ser Glu Phe Asn Thr Tyr Lys Arg 2510 2515 2520 Ser Gly Ile Met Glu Val Asp Arg Ser Glu Ala Lys Glu Gly Leu 2525 2530 2535 Lys Arg Gly Glu Thr Thr Lys His Ala Val Ser Arg Gly Thr Ala 2540 2545 2550 Lys Leu Arg Trp Phe Val Glu Arg Asn Leu Val Lys Pro Glu Gly 2555 2560 2565 Lys Val Ile Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr 2570 2575 2580 Cys Ala Gly Leu Lys Lys Val Thr Glu Val Lys Gly Tyr Thr Lys 2585 2590 2595 Gly Gly Pro Gly His Glu Glu Pro Ile Pro Met Ala Thr Tyr Gly 2600 2605 2610 Trp Asn Leu Val Lys Leu His Ser Gly Lys Asp Val Phe Phe Ile 2615 2620 2625 Pro Pro Glu Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser 2630 2635 2640 Ser Pro Asn Pro Thr Ile Glu Glu Gly Arg Thr Leu Arg Val Leu 2645 2650 2655 Lys Met Val Glu Pro Trp Leu Arg Gly Asn Gln Phe Cys Ile Lys 2660 2665 2670 Ile Leu Asn Pro Tyr Met Pro Ser Val Val Glu Thr Leu Glu Gln 2675 2680 2685 Met Gln Arg Lys His Gly Gly Met Leu Val Arg Asn Pro Leu Ser 2690 2695 2700 Arg Asn Ser Thr His Glu Met Tyr Trp Val Ser Cys Gly Thr Gly 2705 2710 2715 Asn Ile Val Ser Ala Val Asn Met Thr Ser Arg Met Leu Leu Asn 2720 2725 2730 Arg Phe Thr Met Ala His Arg Lys Pro Thr Tyr Glu Arg Asp Val 2735 2740 2745 Asp Leu Gly Ala Gly Thr Arg His Val Ala Val Glu Pro Glu Val 2750 2755 2760 Ala Asn Leu Asp Ile Ile Gly Gln Arg Ile Glu Asn Ile Lys His 2765 2770 2775 Glu His Lys Ser Thr Trp His Tyr Asp Glu Asp Asn Pro Tyr Lys 2780 2785 2790 Thr Trp Ala Tyr His Gly Ser Tyr Glu Val Lys Pro Ser Gly Ser 2795 2800 2805 Ala Ser Ser Met Val Asn Gly Val Val Lys Leu Leu Thr Lys Pro 2810 2815 2820 Trp Asp Val Ile Pro Met Val Thr Gln Ile Ala Met Thr Asp Thr 2825 2830 2835 Thr Pro Phe Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr 2840 2845 2850 Arg Thr Pro Lys Ala Lys Arg Gly Thr Ala Gln Ile Met Glu Val 2855 2860 2865 Thr Ala Arg Trp Leu Trp Gly Phe Leu Ser Arg Asn Lys Lys Pro 2870 2875 2880 Arg Ile Cys Thr Arg Glu Glu Phe Thr Arg Lys Val Arg Ser Asn 2885 2890 2895 Ala Ala Ile Gly Ala Val Phe Val Asp Glu Asn Gln Trp Asn Ser 2900 2905 2910 Ala Lys Glu Ala Val Glu Asp Asp Arg Phe Trp Asp Leu Val His 2915 2920 2925 Arg Glu Arg Glu Leu His Lys Gln Gly Lys Cys Ala Thr Cys Val 2930 2935 2940 Tyr Asn Met Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly 2945 2950 2955 Lys Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala 2960 2965 2970 Arg Phe Leu Glu Phe Glu Ala Leu Gly Phe Met Asn Glu Asp His 2975 2980 2985 Trp Phe Ser Arg Glu Asn Ser Leu Ser Gly Val Glu Gly Glu Gly 2990 2995 3000 Leu His Lys Leu Gly Tyr Ile Leu Arg Asp Ile Ser Lys Ile Pro 3005 3010 3015 Gly Gly Asn Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg 3020 3025 3030 Ile Thr Glu Asp Asp Leu Gln Asn Glu Ala Lys Ile Thr Asp Ile 3035 3040 3045 Met Glu Pro Glu His Ala Leu Leu Ala Lys Ser Ile Phe Lys Leu 3050 3055 3060 Thr Tyr Gln Asn Lys Val Val Arg Val Gln Arg Pro Ala Lys Asn 3065 3070 3075 Gly Thr Val Met Asp Val Ile Ser Arg Arg Asp Gln Arg Gly Ser 3080 3085 3090 Gly Gln Val Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu 3095 3100 3105 Ala Gln Leu Ile Arg Gln Met Glu Ser Glu Gly Ile Phe Ser Pro 3110 3115 3120 Asn Glu Leu Glu Thr Pro Asn Leu Ala Glu Arg Val Leu Asp Trp 3125 3130 3135 Leu Glu Lys Tyr Gly Val Glu Arg Leu Lys Arg Met Ala Ile Ser 3140 3145 3150 Gly Asp Asp Cys Val Val Lys Pro Ile Asp Asp Arg Phe Ala Thr 3155 3160 3165 Ala Leu Thr Ala Leu Asn Asp Met Gly Lys Val Arg Lys Asp Ile 3170 3175 3180 Pro Gln Trp Glu Pro Ser Lys Gly Trp Asn Asp Trp Gln Gln Val 3185 3190 3195 Pro Phe Cys Ser His His Phe His Gln Leu Ile Met Lys Asp Gly 3200 3205 3210 Arg Glu Ile Val Val Pro Cys Arg Asn Gln Asp Glu Leu Val Gly 3215 3220 3225 Arg Ala Arg Val Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr 3230 3235 3240 Ala Cys Leu Gly Lys Ser Tyr Ala Gln Met Trp Gln Leu Met Tyr 3245 3250 3255 Phe His Arg Arg Asp Leu Arg Leu Ala Ala Asn Ala Ile Cys Ser 3260 3265 3270 Ala Val Pro Val Asp Trp Val Pro Thr Ser Arg Thr Thr Trp Ser 3275 3280 3285 Ile His Ala His His Gln Trp Met Thr Thr Glu Asp Met Leu Ser 3290 3295 3300 Val Trp Asn Arg Val Trp Ile Glu Glu Asn Pro Trp Met Glu Asp 3305 3310 3315 Lys Thr Arg Ile Ser Ser Trp Glu Asp Val Pro Tyr Leu Gly Lys 3320 3325 3330 Arg Glu Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ala Arg 3335 3340 3345 Ala Thr Trp Ala Thr Asn Ile Gln Val Ala Ile Asn Gln Val Arg 3350 3355 3360 Arg Leu Ile Gly Asn Glu Asn Tyr Ser Asp Tyr Met Thr Ser Met 3365 3370 3375 Lys Arg Phe Lys Asn Glu Ser Glu Pro Glu Gly Ala Leu Trp 3380 3385 3390 <210> 14 <211> 3391 <212> PRT <213> Dengue virus type 2 <220> <221> VARIANT <222> (363)..(363) <223> Xaa stands for Lys or Glu <220> <221> VARIANT <222> (506)..(506) <223> Xaa stands for Lys or Glu <220> <221> VARIANT <222> (2190)..(2190) <223> Xaa stands for Tyr or Cys <220> <221> VARIANT <222> (2347)..(2347) <223> Xaa stands for Leu or Pro <220> <221> VARIANT <222> (2351)..(2351) <223> Xaa stands for Thr or Ile <220> <221> VARIANT <222> (2828)..(2828) <223> Xaa stands for Thr or Met <220> <221> VARIANT <222> (2894)..(2894) <223> Xaa stands for Val or Gly <400> 14 Met Asn Asn Gln Arg Lys Lys Ala Lys Asn Thr Pro Phe Asn Met Leu 1 5 10 15 Lys Arg Glu Arg Asn Arg Val Ser Thr Val Gln Gln Leu Thr Lys Arg 20 25 30 Phe Ser Leu Gly Met Leu Gln Gly Arg Gly Pro Leu Lys Leu Phe Met 35 40 45 Ala Leu Val Ala Phe Leu Arg Phe Leu Thr Ile Pro Pro Thr Ala Gly 50 55 60 Ile Leu Lys Arg Trp Gly Thr Ile Lys Lys Ser Lys Ala Ile Asn Val 65 70 75 80 Leu Arg Gly Phe Arg Lys Glu Ile Gly Arg Met Leu Asn Ile Leu Asn 85 90 95 Arg Arg Arg Arg Ser Ala Gly Met Ile Ile Met Leu Ile Pro Thr Val 100 105 110 Met Ala Phe His Leu Thr Thr Arg Asn Gly Glu Pro His Met Ile Val 115 120 125 Gly Ile Gln Glu Lys Gly Lys Ser Leu Leu Phe Lys Thr Glu Asn Gly 130 135 140 Val Asn Met Cys Thr Leu Met Ala Met Asp Leu Gly Glu Leu Cys Glu 145 150 155 160 Asp Thr Ile Thr Tyr Lys Cys Pro Leu Leu Arg Gln Asn Glu Pro Glu 165 170 175 Asp Ile Asp Cys Trp Cys Asn Ser Thr Ser Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Thr Thr Thr Gly Glu His Arg Arg Glu Lys Arg Ser Val Ala 195 200 205 Leu Val Pro His Val Gly Met Gly Leu Glu Thr Arg Thr Glu Thr Trp 210 215 220 Met Ser Ser Glu Gly Ala Trp Lys His Ala Gln Arg Ile Glu Thr Trp 225 230 235 240 Ile Leu Arg His Pro Gly Phe Thr Ile Met Ala Ala Ile Leu Ala Tyr 245 250 255 Thr Ile Gly Thr Thr His Phe Gln Arg Val Leu Ile Phe Ile Leu Leu 260 265 270 Thr Ala Val Ala Pro Ser Met Thr Met Arg Cys Ile Gly Ile Ser Asn 275 280 285 Arg Asp Phe Val Glu Gly Val Ser Gly Gly Ser Trp Val Asp Ile Val 290 295 300 Leu Glu His Gly Ser Cys Val Thr Thr Met Ala Lys Asn Lys Pro Thr 305 310 315 320 Leu Asp Phe Glu Leu Ile Lys Thr Glu Ala Lys Gln Pro Ala Thr Leu 325 330 335 Arg Lys Tyr Cys Ile Glu Ala Lys Leu Thr Asn Thr Thr Thr Glu Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Pro Ser Leu Xaa Glu Glu Gln Asp Lys 355 360 365 Arg Phe Val Cys Lys His Ser Met Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Gly Ile Val Thr Cys Ala Met Phe Lys 385 390 395 400 Cys Lys Lys Asn Met Glu Gly Lys Ile Val Gln Pro Glu Asn Leu Glu 405 410 415 Tyr Thr Ile Val Val Thr Pro His Ser Gly Glu Glu His Ala Val Gly 420 425 430 Asn Asp Thr Gly Lys His Gly Lys Glu Ile Lys Val Thr Pro Gln Ser 435 440 445 Ser Ile Thr Glu Ala Glu Leu Thr Gly Tyr Gly Thr Val Thr Met Glu 450 455 460 Cys Ser Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Val Leu Leu Gln 465 470 475 480 Met Glu Asn Lys Ala Trp Leu Val His Arg Gln Trp Phe Leu Asp Leu 485 490 495 Pro Leu Pro Trp Leu Pro Gly Ala Asp Xaa Gln Glu Ser Asn Trp Ile 500 505 510 Gln Lys Glu Thr Leu Val Thr Phe Lys Asn Pro His Ala Lys Lys Gln 515 520 525 Asp Val Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu 530 535 540 Thr Gly Ala Thr Glu Ile Gln Met Ser Ser Gly Asn Leu Leu Phe Thr 545 550 555 560 Gly His Leu Lys Cys Arg Leu Arg Met Asp Lys Leu Gln Leu Lys Gly 565 570 575 Met Ser Tyr Ser Met Cys Thr Gly Lys Phe Lys Val Val Lys Glu Ile 580 585 590 Ala Glu Thr Gln His Gly Thr Ile Val Ile Arg Val Gln Tyr Glu Gly 595 600 605 Asp Gly Ser Pro Cys Lys Ile Pro Phe Glu Ile Met Asp Leu Glu Lys 610 615 620 Arg Tyr Val Leu Gly Arg Leu Ile Thr Val Asn Pro Ile Val Thr Glu 625 630 635 640 Lys Asp Ser Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Asp Ser 645 650 655 Tyr Ile Ile Ile Gly Val Glu Pro Gly Gln Leu Lys Leu Asn Trp Phe 660 665 670 Lys Lys Gly Ser Ser Ile Gly Gln Met Phe Glu Thr Thr Met Arg Gly 675 680 685 Ala Lys Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 690 695 700 Leu Gly Gly Val Phe Thr Ser Ile Gly Lys Ala Leu His Gln Val Phe 705 710 715 720 Gly Ala Ile Tyr Gly Ala Ala Phe Ser Gly Val Ser Trp Thr Met Lys 725 730 735 Ile Leu Ile Gly Val Ile Ile Thr Trp Ile Gly Met Asn Ser Arg Ser 740 745 750 Thr Ser Leu Ser Val Ser Leu Val Leu Val Gly Ile Val Thr Leu Tyr 755 760 765 Leu Gly Val Met Val Gln Ala Asp Ser Gly Cys Val Val Ser Trp Lys 770 775 780 Asn Lys Glu Leu Lys Cys Gly Ser Gly Ile Phe Ile Thr Asp Asn Val 785 790 795 800 His Thr Trp Thr Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ser Lys 805 810 815 Leu Ala Ser Ala Ile Gln Lys Ala Gln Glu Glu Gly Ile Cys Gly Ile 820 825 830 Arg Ser Val Thr Arg Leu Glu Asn Leu Met Trp Lys Gln Ile Thr Pro 835 840 845 Glu Leu Asn His Ile Leu Ala Glu Asn Glu Val Lys Leu Thr Ile Met 850 855 860 Thr Gly Asp Ile Lys Gly Ile Met Gln Ala Gly Lys Arg Ser Leu Arg 865 870 875 880 Pro Gln Pro Thr Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala 885 890 895 Lys Met Leu Ser Thr Glu Ser Tyr Asn Gln Thr Phe Leu Ile Asp Gly 900 905 910 Pro Glu Thr Ala Glu Cys Pro Asn Thr Asn Arg Ala Trp Asn Ser Leu 915 920 925 Glu Val Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu 930 935 940 Lys Leu Lys Glu Lys Gln Asp Ala Phe Cys Asp Ser Lys Leu Met Ser 945 950 955 960 Ala Ala Ile Lys Asp Asn Arg Ala Val His Ala Asp Met Gly Tyr Trp 965 970 975 Ile Glu Ser Ala Leu Asn Asp Thr Trp Lys Ile Glu Lys Ala Ser Phe 980 985 990 Ile Glu Val Lys Asn Cys His Trp Pro Lys Ser His Thr Leu Trp Ser 995 1000 1005 Asn Gly Val Leu Glu Ser Glu Met Ile Ile Pro Lys Asn Leu Ala 1010 1015 1020 Gly Pro Val Ser Gln His Asn Tyr Arg Pro Gly Tyr His Thr Gln 1025 1030 1035 Val Ala Gly Pro Trp His Leu Gly Lys Leu Glu Met Asp Phe Asp 1040 1045 1050 Phe Cys Asp Gly Thr Thr Val Ile Val Thr Glu Asp Cys Gly Asn 1055 1060 1065 Arg Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys Leu Ile 1070 1075 1080 Thr Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Tyr 1085 1090 1095 Arg Gly Glu Asn Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu 1100 1105 1110 Lys Glu Lys Glu Glu Asn Leu Val Asn Ser Leu Val Thr Ala Gly 1115 1120 1125 His Gly Gln Val Asp Asn Phe Ser Leu Gly Val Leu Gly Met Ala 1130 1135 1140 Leu Phe Leu Glu Glu Met Leu Arg Thr Arg Val Gly Thr Lys His 1145 1150 1155 Ala Ile Leu Leu Val Ala Val Ser Phe Val Thr Leu Ile Thr Gly 1160 1165 1170 Asn Met Ser Phe Lys Asp Leu Gly Arg Val Val Val Met Val Gly 1175 1180 1185 Ala Thr Met Ala Asp Asp Ile Gly Met Gly Val Thr Tyr Leu Ala 1190 1195 1200 Leu Leu Ala Ala Phe Lys Val Arg Pro Thr Phe Ala Ala Gly Leu 1205 1210 1215 Leu Leu Arg Lys Leu Thr Ser Lys Glu Leu Met Met Thr Thr Ile 1220 1225 1230 Gly Ile Val Leu Leu Ser Gln Ser Thr Ile Pro Glu Thr Val Leu 1235 1240 1245 Glu Leu Thr Asp Ala Leu Ala Leu Gly Met Met Val Leu Lys Ile 1250 1255 1260 Val Arg Asn Met Glu Lys Tyr Gln Leu Ala Val Thr Ile Met Ala 1265 1270 1275 Ile Leu Cys Val Pro Asn Ala Val Ile Leu Gln Asn Ala Trp Lys 1280 1285 1290 Val Ser Cys Thr Ile Leu Ala Val Val Ser Val Ser Pro Leu Phe 1295 1300 1305 Leu Thr Ser Ser Gln Gln Lys Thr Asp Trp Ile Pro Leu Ala Leu 1310 1315 1320 Thr Ile Lys Gly Leu Asn Pro Thr Ala Ile Phe Leu Thr Thr Leu 1325 1330 1335 Ser Arg Thr Asn Lys Lys Arg Ser Trp Pro Leu Asn Glu Ala Ile 1340 1345 1350 Met Ala Val Gly Met Val Ser Ile Leu Ala Ser Ser Leu Leu Lys 1355 1360 1365 Asn Asp Ile Pro Met Thr Gly Pro Leu Val Ala Gly Gly Leu Leu 1370 1375 1380 Thr Val Cys Tyr Val Leu Thr Gly Arg Ser Ala Asp Leu Glu Leu 1385 1390 1395 Glu Arg Ala Thr Asp Val Lys Trp Glu Asp Gln Ala Glu Ile Ser 1400 1405 1410 Gly Ser Ser Pro Ile Leu Ser Ile Thr Ile Ser Glu Asp Gly Ser 1415 1420 1425 Met Ser Ile Lys Asn Glu Glu Glu Glu Gln Thr Leu Thr Ile Leu 1430 1435 1440 Ile Arg Thr Gly Leu Leu Val Ile Ser Gly Leu Phe Pro Ile Ser 1445 1450 1455 Ile Pro Val Thr Ala Ala Ala Trp Tyr Leu Trp Glu Val Lys Lys 1460 1465 1470 Gln Arg Ala Gly Val Leu Trp Asp Val Pro Ser Pro Pro Pro Met 1475 1480 1485 Gly Lys Ala Glu Leu Glu Asp Gly Ala Tyr Arg Ile Lys Gln Lys 1490 1495 1500 Gly Ile Leu Gly Tyr Ser Gln Ile Gly Ala Gly Val Tyr Lys Glu 1505 1510 1515 Gly Thr Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu 1520 1525 1530 Met His Lys Gly Lys Arg Ile Glu Pro Ser Trp Ala Asp Val Lys 1535 1540 1545 Lys Asp Leu Ile Ser Tyr Gly Gly Gly Trp Lys Leu Glu Gly Glu 1550 1555 1560 Trp Lys Glu Gly Glu Glu Val Gln Val Leu Ala Leu Glu Pro Gly 1565 1570 1575 Lys Asn Pro Arg Ala Val Gln Thr Lys Pro Gly Leu Phe Lys Thr 1580 1585 1590 Asn Thr Gly Thr Ile Gly Ala Val Ser Leu Asp Phe Ser Pro Gly 1595 1600 1605 Thr Ser Gly Ser Pro Ile Ile Asp Lys Lys Gly Lys Val Val Gly 1610 1615 1620 Leu Tyr Gly Asn Gly Val Val Thr Arg Ser Gly Ala Tyr Val Ser 1625 1630 1635 Ala Ile Ala Gln Thr Glu Lys Ser Ile Glu Asp Asn Pro Glu Ile 1640 1645 1650 Lys Asp Asp Ile Phe Arg Lys Arg Arg Leu Thr Ile Met Asp Leu 1655 1660 1665 His Pro Gly Ala Gly Lys Thr Lys Arg Tyr Leu Pro Ala Ile Val 1670 1675 1680 Arg Glu Ala Ile Lys Arg Gly Leu Arg Thr Leu Ile Leu Ala Pro 1685 1690 1695 Thr Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu 1700 1705 1710 Pro Ile Arg Tyr Gln Thr Pro Ala Ile Arg Ala Glu His Thr Gly 1715 1720 1725 Arg Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met Arg 1730 1735 1740 Leu Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Leu Ile Ile Met 1745 1750 1755 Asp Glu Ala His Phe Thr Asp Pro Ala Ser Ile Ala Ala Arg Gly 1760 1765 1770 Tyr Ile Ser Thr Arg Val Glu Met Gly Glu Ala Ala Gly Ile Phe 1775 1780 1785 Met Thr Ala Thr Pro Pro Gly Ser Arg Asp Pro Phe Pro Gln Ser 1790 1795 1800 Asn Ala Pro Ile Ile Asp Glu Glu Arg Glu Ile Pro Glu Arg Ser 1805 1810 1815 Trp Asn Ser Gly His Glu Trp Val Thr Asp Phe Lys Gly Lys Thr 1820 1825 1830 Val Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Ala 1835 1840 1845 Cys Leu Arg Lys Asn Gly Lys Lys Val Ile Gln Leu Ser Arg Lys 1850 1855 1860 Thr Phe Asp Ser Glu Tyr Val Lys Thr Arg Thr Asn Asp Trp Asp 1865 1870 1875 Phe Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys 1880 1885 1890 Ala Glu Arg Val Ile Asp Pro Arg Arg Cys Met Lys Pro Val Ile 1895 1900 1905 Leu Thr Asp Gly Glu Glu Arg Val Ile Leu Ala Gly Pro Met Pro 1910 1915 1920 Val Thr His Ser Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg 1925 1930 1935 Asn Pro Lys Asn Glu Asn Asp Gln Tyr Ile Tyr Met Gly Glu Pro 1940 1945 1950 Leu Glu Asn Asp Glu Asp Cys Ala His Trp Lys Glu Ala Lys Met 1955 1960 1965 Leu Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ser Met 1970 1975 1980 Phe Glu Pro Glu Arg Glu Lys Val Asp Ala Ile Asp Gly Glu Tyr 1985 1990 1995 Arg Leu Arg Gly Glu Ala Arg Lys Thr Phe Val Asp Leu Met Arg 2000 2005 2010 Arg Gly Asp Leu Pro Val Trp Leu Ala Tyr Lys Val Ala Ala Glu 2015 2020 2025 Gly Ile Asn Tyr Ala Asp Arg Arg Trp Cys Phe Asp Gly Ile Lys 2030 2035 2040 Asn Asn Gln Ile Leu Glu Glu Asn Val Glu Val Glu Ile Trp Thr 2045 2050 2055 Lys Glu Gly Glu Arg Lys Lys Leu Lys Pro Arg Trp Leu Asp Ala 2060 2065 2070 Arg Ile Tyr Ser Asp Pro Leu Ala Leu Lys Glu Phe Lys Glu Phe 2075 2080 2085 Ala Ala Gly Arg Lys Ser Leu Thr Leu Asn Leu Ile Thr Glu Met 2090 2095 2100 Gly Arg Leu Pro Thr Phe Met Thr Gln Lys Thr Arg Asp Ala Leu 2105 2110 2115 Asp Asn Leu Ala Val Leu His Thr Ala Glu Ala Gly Gly Arg Ala 2120 2125 2130 Tyr Asn His Ala Leu Ser Glu Leu Pro Glu Thr Leu Glu Thr Leu 2135 2140 2145 Leu Leu Leu Thr Leu Leu Ala Thr Val Thr Gly Gly Ile Phe Leu 2150 2155 2160 Phe Leu Met Ser Gly Arg Gly Ile Gly Lys Met Thr Leu Gly Met 2165 2170 2175 Cys Cys Ile Ile Thr Ala Ser Ile Leu Leu Trp Xaa Ala Gln Ile 2180 2185 2190 Gln Pro His Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe Leu 2195 2200 2205 Ile Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln 2210 2215 2220 Asp Asn Gln Leu Thr Tyr Val Val Ile Ala Ile Leu Thr Val Val 2225 2230 2235 Ala Ala Thr Met Ala Asn Glu Met Gly Phe Leu Glu Lys Thr Lys 2240 2245 2250 Lys Asp Leu Gly Leu Gly Ser Ile Ala Thr Gln Gln Pro Glu Ser 2255 2260 2265 Asn Ile Leu Asp Ile Asp Leu Arg Pro Ala Ser Ala Trp Thr Leu 2270 2275 2280 Tyr Ala Val Ala Thr Thr Phe Val Thr Pro Met Leu Arg His Ser 2285 2290 2295 Ile Glu Asn Ser Ser Val Asn Val Ser Leu Thr Ala Ile Ala Asn 2300 2305 2310 Gln Ala Thr Val Leu Met Gly Leu Gly Lys Gly Trp Pro Leu Ser 2315 2320 2325 Lys Met Asp Ile Gly Val Pro Leu Leu Ala Ile Gly Cys Tyr Ser 2330 2335 2340 Gln Val Asn Xaa Ile Thr Leu Xaa Ala Ala Leu Leu Leu Leu Val 2345 2350 2355 Ala His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr 2360 2365 2370 Arg Glu Ala Gln Lys Arg Ala Ala Ala Gly Ile Met Lys Asn Pro 2375 2380 2385 Thr Val Asp Gly Ile Thr Val Ile Asp Leu Asp Pro Ile Pro Tyr 2390 2395 2400 Asp Pro Lys Phe Glu Lys Gln Leu Gly Gln Val Met Leu Leu Ile 2405 2410 2415 Leu Cys Val Thr Gln Val Leu Met Met Arg Thr Thr Trp Ala Leu 2420 2425 2430 Cys Glu Ala Leu Thr Leu Ala Thr Gly Pro Ile Ser Thr Leu Trp 2435 2440 2445 Glu Gly Asn Pro Gly Arg Phe Trp Asn Thr Thr Ile Ala Val Ser 2450 2455 2460 Met Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu 2465 2470 2475 Leu Phe Ser Ile Met Lys Asn Thr Thr Asn Thr Arg Arg Gly Thr 2480 2485 2490 Gly Asn Ile Gly Glu Thr Leu Gly Glu Lys Trp Lys Ser Arg Leu 2495 2500 2505 Asn Ala Leu Gly Lys Ser Glu Phe Gln Ile Tyr Lys Lys Ser Gly 2510 2515 2520 Ile Gln Glu Val Asp Arg Thr Leu Ala Lys Glu Gly Ile Lys Arg 2525 2530 2535 Gly Glu Thr Asp His His Ala Val Ser Arg Gly Ser Ala Lys Leu 2540 2545 2550 Arg Trp Phe Val Glu Arg Asn Met Val Thr Pro Glu Gly Lys Val 2555 2560 2565 Val Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Cys Gly 2570 2575 2580 Gly Leu Lys Asn Val Arg Glu Val Lys Gly Leu Thr Lys Gly Gly 2585 2590 2595 Pro Gly His Glu Glu Pro Ile Pro Met Ser Thr Tyr Gly Trp Asn 2600 2605 2610 Leu Val Arg Leu Gln Ser Gly Val Asp Val Phe Phe Ile Pro Pro 2615 2620 2625 Glu Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Pro 2630 2635 2640 Asn Pro Thr Val Glu Ala Gly Arg Thr Leu Arg Val Leu Asn Leu 2645 2650 2655 Val Glu Asn Trp Leu Asn Asn Asn Thr Gln Phe Cys Ile Lys Val 2660 2665 2670 Leu Asn Pro Tyr Met Pro Ser Val Ile Glu Lys Met Glu Ala Leu 2675 2680 2685 Gln Arg Lys Tyr Gly Gly Ala Leu Val Arg Asn Pro Leu Ser Arg 2690 2695 2700 Asn Ser Thr His Glu Met Tyr Trp Val Ser Asn Ala Ser Gly Asn 2705 2710 2715 Ile Val Ser Ser Val Asn Met Ile Ser Arg Met Leu Ile Asn Arg 2720 2725 2730 Phe Thr Met Arg His Lys Lys Ala Thr Tyr Glu Pro Asp Val Asp 2735 2740 2745 Leu Gly Ser Gly Thr Arg Asn Ile Gly Val Glu Ser Glu Ile Pro 2750 2755 2760 Asn Leu Asp Ile Ile Gly Lys Arg Ile Glu Lys Ile Lys Gln Glu 2765 2770 2775 His Glu Thr Ser Trp His Tyr Asp Gln Asp His Pro Tyr Lys Thr 2780 2785 2790 Trp Ala Tyr His Gly Ser Tyr Glu Thr Lys Gln Thr Gly Ser Ala 2795 2800 2805 Ser Ser Met Val Asn Gly Val Val Arg Leu Leu Thr Lys Pro Trp 2810 2815 2820 Asp Val Leu Pro Xaa Val Thr Gln Met Ala Met Thr Asp Thr Thr 2825 2830 2835 Pro Phe Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg 2840 2845 2850 Thr Gln Glu Pro Lys Glu Gly Thr Lys Lys Leu Met Arg Ile Thr 2855 2860 2865 Ala Glu Trp Leu Trp Lys Glu Leu Gly Lys Lys Lys Thr Pro Arg 2870 2875 2880 Met Cys Thr Arg Glu Glu Phe Thr Arg Lys Xaa Arg Ser Asn Ala 2885 2890 2895 Ala Leu Gly Ala Ile Phe Thr Asp Glu Asn Lys Trp Lys Ser Ala 2900 2905 2910 Arg Glu Ala Val Glu Asp Ser Arg Phe Trp Glu Leu Val Asp Lys 2915 2920 2925 Glu Arg Asn Leu His Leu Glu Gly Lys Cys Glu Thr Cys Val Tyr 2930 2935 2940 Asn Met Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Lys 2945 2950 2955 Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg 2960 2965 2970 Phe Leu Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp 2975 2980 2985 Phe Ser Arg Glu Asn Ser Leu Ser Gly Val Glu Gly Glu Gly Leu 2990 2995 3000 His Lys Leu Gly Tyr Ile Leu Arg Asp Val Ser Lys Lys Glu Gly 3005 3010 3015 Gly Ala Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile 3020 3025 3030 Thr Leu Glu Asp Leu Lys Asn Glu Glu Met Val Thr Asn His Met 3035 3040 3045 Glu Gly Glu His Lys Lys Leu Ala Glu Ala Ile Phe Lys Leu Thr 3050 3055 3060 Tyr Gln Asn Lys Val Val Arg Val Gln Arg Pro Thr Pro Arg Gly 3065 3070 3075 Thr Val Met Asp Ile Ile Ser Arg Arg Asp Gln Arg Gly Ser Gly 3080 3085 3090 Gln Val Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Ala 3095 3100 3105 Gln Leu Ile Arg Gln Met Glu Gly Glu Gly Val Phe Lys Asn Ile 3110 3115 3120 Gln His Leu Thr Val Thr Glu Glu Val Ala Val Gln Asn Trp Leu 3125 3130 3135 Ala Arg Val Gly Arg Glu Arg Leu Ser Arg Met Ala Ile Ser Gly 3140 3145 3150 Asp Asp Cys Val Val Lys Pro Leu Asp Asp Arg Phe Ala Asn Ala 3155 3160 3165 Leu Thr Ala Leu Asn Asp Met Gly Lys Ile Arg Lys Asp Ile Gln 3170 3175 3180 Gln Trp Glu Pro Ser Lys Gly Trp Asn Asp Trp Thr Gln Val Pro 3185 3190 3195 Phe Cys Ser His His Phe His Glu Leu Ile Met Lys Asp Gly Arg 3200 3205 3210 Val Leu Val Val Pro Cys Arg Asn Gln Asp Glu Leu Ile Gly Arg 3215 3220 3225 Ala Arg Ile Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala 3230 3235 3240 Cys Leu Gly Lys Ser Tyr Ala Gln Met Trp Ser Leu Met Tyr Phe 3245 3250 3255 His Arg Arg Asp Leu Arg Leu Ala Ala Asn Ala Ile Cys Ser Ala 3260 3265 3270 Val Pro Ser His Trp Val Pro Thr Ser Arg Thr Thr Trp Ser Ile 3275 3280 3285 His Ala Lys His Glu Trp Met Thr Thr Glu Asp Met Leu Thr Val 3290 3295 3300 Trp Asn Arg Val Trp Ile Arg Glu Asn Pro Trp Met Glu Asp Lys 3305 3310 3315 Thr Pro Val Glu Ser Trp Glu Glu Ile Pro Tyr Leu Gly Lys Arg 3320 3325 3330 Glu Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ser Arg Ala 3335 3340 3345 Thr Trp Ala Lys Asn Ile Gln Ala Ala Ile Asn Gln Val Arg Ser 3350 3355 3360 Leu Ile Gly Asn Glu Glu Tyr Thr Asp Tyr Met Pro Ser Met Lys 3365 3370 3375 Arg Phe Arg Arg Glu Glu Glu Glu Ala Gly Val Leu Trp 3380 3385 3390 <210> 15 <211> 3390 <212> PRT <213> Dengue virus type 3 <220> <221> VARIANT <222> (764)..(764) <223> Xaa stands for Thr or Ile <400> 15 Met Asn Asn Gln Arg Lys Lys Thr Gly Lys Pro Ser Ile Asn Met Leu 1 5 10 15 Lys Arg Val Arg Asn Arg Val Ser Thr Gly Ser Gln Leu Ala Lys Arg 20 25 30 Phe Ser Arg Gly Leu Leu Asn Gly Gln Gly Pro Met Lys Leu Val Met 35 40 45 Ala Phe Ile Ala Phe Leu Arg Phe Leu Ala Ile Pro Pro Thr Ala Gly 50 55 60 Val Leu Ala Arg Trp Gly Thr Phe Lys Lys Ser Gly Ala Ile Lys Val 65 70 75 80 Leu Lys Gly Phe Lys Lys Glu Ile Ser Asn Met Leu Ser Ile Ile Asn 85 90 95 Lys Arg Lys Lys Thr Ser Leu Cys Leu Met Met Met Leu Pro Ala Thr 100 105 110 Leu Ala Phe His Leu Thr Ser Arg Asp Gly Glu Pro Arg Met Ile Val 115 120 125 Gly Lys Asn Glu Arg Gly Lys Ser Leu Leu Phe Lys Thr Ala Ser Gly 130 135 140 Ile Asn Met Cys Thr Leu Ile Ala Met Asp Leu Gly Glu Met Cys Asp 145 150 155 160 Asp Thr Val Thr Tyr Lys Cys Pro His Ile Thr Glu Val Glu Pro Glu 165 170 175 Asp Ile Asp Cys Trp Cys Asn Leu Thr Ser Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Asn Gln Ala Gly Glu His Arg Arg Asp Lys Arg Ser Val Ala 195 200 205 Leu Ala Pro His Val Gly Met Gly Leu Asp Thr Arg Thr Gln Thr Trp 210 215 220 Met Ser Ala Glu Gly Ala Trp Arg Gln Val Glu Lys Val Glu Thr Trp 225 230 235 240 Ala Leu Arg His Pro Gly Phe Thr Ile Leu Ala Leu Phe Leu Ala His 245 250 255 Tyr Ile Gly Thr Ser Leu Thr Gln Lys Val Val Ile Phe Ile Leu Leu 260 265 270 Met Leu Val Thr Pro Ser Met Thr Met Arg Cys Val Gly Val Gly Asn 275 280 285 Arg Asp Phe Val Glu Gly Leu Ser Gly Ala Thr Trp Val Asp Val Val 290 295 300 Leu Glu His Gly Gly Cys Val Thr Thr Met Ala Lys Asn Lys Pro Thr 305 310 315 320 Leu Asp Ile Glu Leu Gln Lys Thr Glu Ala Thr Gln Leu Ala Thr Leu 325 330 335 Arg Lys Leu Cys Ile Glu Gly Lys Ile Thr Asn Ile Thr Thr Asp Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Ala Ile Leu Pro Glu Glu Gln Asp Gln 355 360 365 Asn Tyr Val Cys Lys His Thr Tyr Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Gln 385 390 395 400 Cys Leu Glu Ser Ile Glu Gly Lys Val Val Gln His Glu Asn Leu Lys 405 410 415 Tyr Thr Val Ile Ile Thr Val His Thr Gly Asp Gln His Gln Val Gly 420 425 430 Asn Glu Thr Gln Gly Val Thr Ala Glu Ile Thr Pro Gln Ala Ser Thr 435 440 445 Ala Glu Ala Ile Leu Pro Glu Tyr Gly Thr Leu Gly Leu Glu Cys Ser 450 455 460 Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Ile Ser Leu Thr Met Lys 465 470 475 480 Asn Lys Ala Trp Met Val His Arg Gln Trp Phe Phe Asp Leu Pro Leu 485 490 495 Pro Trp Thr Ser Gly Ala Thr Ala Glu Thr Pro Thr Trp Asn Arg Lys 500 505 510 Glu Leu Leu Val Thr Phe Lys Asn Ala His Ala Lys Lys Gln Glu Val 515 520 525 Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu Thr Gly 530 535 540 Ala Thr Glu Ile Gln Thr Ser Gly Gly Thr Ser Ile Phe Ala Gly His 545 550 555 560 Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Glu Leu Lys Gly Met Ser 565 570 575 Tyr Ala Met Cys Leu Gly Ser Phe Val Leu Lys Lys Glu Val Ser Glu 580 585 590 Thr Gln His Gly Thr Ile Leu Ile Lys Val Glu Tyr Lys Gly Glu Asp 595 600 605 Ala Pro Cys Lys Ile Pro Phe Ser Thr Glu Asp Gly Gln Gly Lys Ala 610 615 620 His Asn Gly Arg Leu Ile Thr Ala Asn Pro Val Val Thr Lys Lys Glu 625 630 635 640 Glu Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser Asn Ile 645 650 655 Val Ile Gly Ile Gly Asp Lys Ala Leu Lys Ile Asn Trp Tyr Lys Lys 660 665 670 Gly Ser Ser Ile Gly Lys Met Phe Glu Ala Thr Ala Arg Gly Val Arg 675 680 685 Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly 690 695 700 Gly Val Leu Asn Ser Leu Gly Lys Met Val His Gln Ile Phe Gly Ser 705 710 715 720 Ala Tyr Thr Ala Leu Phe Gly Gly Val Ser Trp Met Met Lys Ile Gly 725 730 735 Ile Gly Val Leu Leu Thr Trp Ile Gly Leu Asn Ser Lys Asn Thr Ser 740 745 750 Met Ser Phe Ser Cys Ile Ala Ile Gly Ile Ile Xaa Leu Tyr Leu Gly 755 760 765 Ala Val Val Gln Ala Asp Met Gly Cys Val Ile Asn Trp Lys Gly Lys 770 775 780 Glu Leu Lys Cys Gly Ser Gly Ile Phe Val Thr Asn Glu Val His Thr 785 790 795 800 Trp Thr Glu Gln Tyr Lys Phe Gln Ala Asp Ser Pro Lys Arg Leu Ala 805 810 815 Thr Ala Ile Ala Gly Ala Trp Glu Asn Gly Val Cys Gly Ile Arg Ser 820 825 830 Thr Thr Arg Met Glu Asn Leu Leu Trp Lys Gln Ile Ala Asn Glu Leu 835 840 845 Asn Tyr Ile Leu Trp Glu Asn Asn Ile Lys Leu Thr Val Val Val Gly 850 855 860 Asp Ile Thr Gly Val Leu Glu Gln Gly Lys Arg Thr Leu Thr Pro Gln 865 870 875 880 Pro Met Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala Lys Ile 885 890 895 Val Thr Ala Glu Thr Gln Asn Ser Ser Phe Ile Ile Asp Gly Pro Ser 900 905 910 Thr Pro Glu Cys Pro Ser Ala Ser Arg Ala Trp Asn Val Trp Glu Val 915 920 925 Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu Lys Leu 930 935 940 Arg Glu Val Tyr Thr Gln Leu Cys Asp His Arg Leu Met Ser Ala Ala 945 950 955 960 Val Lys Asp Glu Arg Ala Val His Ala Asp Met Gly Tyr Trp Ile Glu 965 970 975 Ser Gln Lys Asn Gly Ser Trp Lys Leu Glu Lys Ala Ser Phe Ile Glu 980 985 990 Val Lys Thr Cys Thr Trp Pro Lys Ser His Thr Leu Trp Ser Asn Gly 995 1000 1005 Val Leu Glu Ser Asp Met Ile Ile Pro Lys Ser Leu Ala Gly Pro 1010 1015 1020 Ile Ser Gln His Asn His Arg Pro Gly Tyr His Thr Gln Thr Ala 1025 1030 1035 Gly Pro Trp His Leu Gly Lys Leu Glu Leu Asp Phe Asn Tyr Cys 1040 1045 1050 Glu Gly Thr Thr Val Val Ile Ser Glu Asn Cys Gly Thr Arg Gly 1055 1060 1065 Pro Ser Leu Arg Thr Thr Thr Val Ser Gly Lys Leu Ile His Glu 1070 1075 1080 Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Tyr Met Gly 1085 1090 1095 Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Ile Asn Glu 1100 1105 1110 Lys Glu Glu Asn Met Val Lys Ser Leu Ala Ser Ala Gly Ser Gly 1115 1120 1125 Lys Val Asp Asn Phe Thr Met Gly Val Leu Cys Leu Ala Ile Leu 1130 1135 1140 Phe Glu Glu Val Met Arg Gly Lys Phe Gly Lys Lys His Met Ile 1145 1150 1155 Ala Gly Val Leu Phe Thr Phe Val Leu Leu Leu Ser Gly Gln Ile 1160 1165 1170 Thr Trp Arg Asp Met Ala His Thr Leu Ile Met Ile Gly Ser Asn 1175 1180 1185 Ala Ser Asp Arg Met Gly Met Gly Val Thr Tyr Leu Ala Leu Ile 1190 1195 1200 Ala Thr Phe Lys Ile Gln Pro Leu Leu Ala Leu Gly Phe Phe Leu 1205 1210 1215 Arg Lys Leu Thr Ser Arg Glu Asn Leu Leu Leu Gly Val Gly Leu 1220 1225 1230 Ala Met Ala Thr Thr Leu Arg Leu Pro Glu Asp Ile Glu Gln Met 1235 1240 1245 Ala Asn Gly Ile Ala Leu Gly Leu Met Ala Leu Lys Leu Ile Thr 1250 1255 1260 Gln Phe Glu Thr Tyr Gln Leu Trp Thr Ala Leu Val Ser Leu Thr 1265 1270 1275 Cys Ser Asn Thr Ile Phe Thr Leu Thr Val Ala Trp Arg Thr Ala 1280 1285 1290 Thr Leu Ile Leu Ala Gly Ile Ser Leu Leu Pro Val Cys Gln Ser 1295 1300 1305 Ser Ser Met Arg Lys Thr Asp Trp Leu Pro Met Thr Val Ala Ala 1310 1315 1320 Met Gly Ala Gln Pro Leu Pro Leu Phe Ile Phe Ser Leu Lys Asp 1325 1330 1335 Thr Leu Lys Arg Arg Ser Trp Pro Leu Asn Glu Gly Val Met Ala 1340 1345 1350 Val Gly Leu Val Ser Ile Leu Ala Ser Ser Leu Leu Arg Asn Asp 1355 1360 1365 Val Pro Met Ala Gly Pro Leu Val Ala Gly Gly Leu Leu Ile Ala 1370 1375 1380 Cys Tyr Val Ile Thr Gly Thr Ser Ala Asp Leu Thr Val Glu Lys 1385 1390 1395 Ala Ala Asp Val Thr Trp Glu Glu Glu Ala Glu Gln Thr Gly Val 1400 1405 1410 Ser His Asn Leu Met Val Thr Val Asp Asp Asp Gly Thr Met Arg 1415 1420 1425 Ile Lys Asp Asp Glu Thr Glu Asn Ile Leu Thr Val Leu Leu Lys 1430 1435 1440 Thr Ala Leu Leu Ile Val Ser Gly Ile Phe Pro Tyr Ser Ile Pro 1445 1450 1455 Ala Thr Leu Leu Val Trp His Thr Trp Gln Lys Gln Thr Gln Arg 1460 1465 1470 Ser Gly Val Leu Trp Asp Val Pro Ser Pro Pro Glu Thr Gln Lys 1475 1480 1485 Ala Glu Leu Glu Glu Gly Val Tyr Arg Ile Lys Gln Gln Gly Ile 1490 1495 1500 Phe Gly Lys Thr Gln Val Gly Val Gly Val Gln Lys Glu Gly Val 1505 1510 1515 Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu Thr His 1520 1525 1530 Asn Gly Lys Arg Leu Glu Pro Asn Trp Ala Ser Val Lys Lys Asp 1535 1540 1545 Leu Ile Ser Tyr Gly Gly Gly Trp Arg Leu Ser Ala Gln Trp Lys 1550 1555 1560 Lys Gly Glu Glu Val Gln Val Ile Ala Val Glu Pro Gly Lys Asn 1565 1570 1575 Pro Lys Asn Phe Gln Thr Met Pro Gly Ile Phe Gln Thr Thr Thr 1580 1585 1590 Gly Glu Ile Gly Ala Ile Ala Leu Asp Phe Lys Pro Gly Thr Ser 1595 1600 1605 Gly Ser Pro Ile Ile Asn Arg Glu Gly Lys Val Val Gly Leu Tyr 1610 1615 1620 Gly Asn Gly Val Val Thr Lys Asn Gly Gly Tyr Val Ser Gly Ile 1625 1630 1635 Ala Gln Thr Asn Ala Glu Pro Asp Gly Pro Thr Pro Glu Leu Glu 1640 1645 1650 Glu Glu Met Phe Lys Lys Arg Asn Leu Thr Ile Met Asp Leu His 1655 1660 1665 Pro Gly Ser Gly Lys Thr Arg Lys Tyr Leu Pro Ala Ile Val Arg 1670 1675 1680 Glu Ala Ile Lys Arg Arg Leu Arg Thr Leu Ile Leu Ala Pro Thr 1685 1690 1695 Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Lys Gly Leu Pro 1700 1705 1710 Ile Arg Tyr Gln Thr Thr Ala Thr Lys Ser Glu His Thr Gly Arg 1715 1720 1725 Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met Arg Leu 1730 1735 1740 Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Leu Ile Ile Met Asp 1745 1750 1755 Glu Ala His Phe Thr Asp Pro Ala Ser Ile Ala Ala Arg Gly Tyr 1760 1765 1770 Ile Ser Thr Arg Val Gly Met Gly Glu Ala Ala Ala Ile Phe Met 1775 1780 1785 Thr Ala Thr Pro Pro Gly Thr Ala Asp Ala Phe Pro Gln Ser Asn 1790 1795 1800 Ala Pro Ile Gln Asp Glu Glu Arg Asp Ile Pro Glu Arg Ser Trp 1805 1810 1815 Asn Ser Gly Asn Glu Trp Ile Thr Asp Phe Val Gly Lys Thr Val 1820 1825 1830 Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Asn Cys 1835 1840 1845 Leu Arg Lys Asn Gly Lys Lys Val Ile Gln Leu Ser Arg Lys Thr 1850 1855 1860 Phe Asp Thr Glu Tyr Gln Lys Thr Lys Leu Asn Asp Trp Asp Phe 1865 1870 1875 Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys Ala 1880 1885 1890 Asp Arg Val Ile Asp Pro Arg Arg Cys Leu Lys Pro Val Ile Leu 1895 1900 1905 Thr Asp Gly Pro Glu Arg Val Ile Leu Ala Gly Pro Met Pro Val 1910 1915 1920 Thr Val Ala Ser Ala Ala Gln Arg Arg Gly Arg Val Gly Arg Asn 1925 1930 1935 Pro Gln Lys Glu Asn Asp Gln Tyr Ile Phe Met Gly Gln Pro Leu 1940 1945 1950 Asn Asn Asp Glu Asp His Ala His Trp Thr Glu Ala Lys Met Leu 1955 1960 1965 Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ala Leu Phe 1970 1975 1980 Glu Pro Glu Arg Glu Lys Ser Ala Ala Ile Asp Gly Glu Tyr Arg 1985 1990 1995 Leu Lys Gly Glu Ser Arg Lys Thr Phe Val Glu Leu Met Arg Arg 2000 2005 2010 Gly Asp Leu Pro Val Trp Leu Ala His Lys Val Ala Ser Glu Gly 2015 2020 2025 Ile Lys Tyr Thr Asp Arg Lys Trp Cys Phe Asp Gly Glu Arg Asn 2030 2035 2040 Asn Gln Ile Leu Glu Glu Asn Met Asp Val Glu Ile Trp Thr Lys 2045 2050 2055 Glu Gly Glu Lys Lys Lys Leu Arg Pro Arg Trp Leu Asp Ala Arg 2060 2065 2070 Thr Tyr Ser Asp Pro Leu Ala Leu Lys Glu Phe Lys Asp Phe Ala 2075 2080 2085 Ala Gly Arg Lys Ser Ile Ala Leu Asp Leu Val Thr Glu Ile Gly 2090 2095 2100 Arg Val Pro Ser His Leu Ala His Arg Thr Arg Asn Ala Leu Asp 2105 2110 2115 Asn Leu Val Met Leu His Thr Ser Glu His Gly Gly Arg Ala Tyr 2120 2125 2130 Arg His Ala Val Glu Glu Leu Pro Glu Thr Met Glu Thr Leu Leu 2135 2140 2145 Leu Leu Gly Leu Met Ile Leu Leu Thr Gly Gly Ala Met Leu Phe 2150 2155 2160 Leu Ile Ser Gly Lys Gly Ile Gly Lys Thr Ser Ile Gly Leu Ile 2165 2170 2175 Cys Val Ile Ala Ser Ser Gly Met Leu Trp Met Ala Asp Val Pro 2180 2185 2190 Leu Gln Trp Ile Ala Ser Ala Ile Val Leu Glu Phe Phe Met Met 2195 2200 2205 Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln Asp 2210 2215 2220 Asn Gln Leu Ala Tyr Val Val Ile Gly Ile Leu Thr Leu Ala Ala 2225 2230 2235 Ile Val Ala Ala Asn Glu Met Gly Leu Leu Glu Thr Thr Lys Arg 2240 2245 2250 Asp Leu Gly Met Ser Lys Glu Pro Gly Val Val Ser Pro Thr Ser 2255 2260 2265 Tyr Leu Asp Val Asp Leu His Pro Ala Ser Ala Trp Thr Leu Tyr 2270 2275 2280 Ala Val Ala Thr Thr Val Ile Thr Pro Met Leu Arg His Thr Ile 2285 2290 2295 Glu Asn Ser Thr Ala Asn Val Ser Leu Ala Ala Ile Ala Asn Gln 2300 2305 2310 Ala Val Val Leu Met Gly Leu Asp Lys Gly Trp Pro Ile Ser Lys 2315 2320 2325 Met Asp Leu Gly Val Pro Leu Leu Ala Leu Gly Cys Tyr Ser Gln 2330 2335 2340 Val Asn Pro Leu Thr Leu Ala Ala Ala Val Leu Leu Leu Val Thr 2345 2350 2355 His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr Arg 2360 2365 2370 Glu Ala Gln Lys Arg Thr Ala Ala Gly Ile Met Lys Asn Pro Thr 2375 2380 2385 Val Asp Gly Ile Met Thr Ile Asp Leu Asp Pro Val Ile Tyr Asp 2390 2395 2400 Ser Lys Phe Glu Lys Gln Leu Gly Gln Val Met Leu Leu Val Leu 2405 2410 2415 Cys Ala Val Gln Leu Leu Leu Met Arg Thr Ser Trp Ala Leu Cys 2420 2425 2430 Glu Val Leu Thr Leu Ala Thr Gly Pro Ile Thr Thr Leu Trp Glu 2435 2440 2445 Gly Ser Pro Gly Lys Phe Trp Asn Thr Thr Ile Ala Val Ser Met 2450 2455 2460 Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu Ala 2465 2470 2475 Phe Ser Ile Met Lys Ser Val Gly Thr Gly Lys Arg Gly Thr Gly 2480 2485 2490 Ser Gln Gly Glu Thr Leu Gly Glu Lys Trp Lys Lys Lys Leu Asn 2495 2500 2505 Gln Leu Ser Arg Lys Glu Phe Asp Leu Tyr Lys Lys Ser Gly Ile 2510 2515 2520 Thr Glu Val Asp Arg Thr Glu Ala Lys Glu Gly Leu Lys Arg Gly 2525 2530 2535 Glu Ile Thr His His Ala Val Ser Arg Gly Ser Ala Lys Leu Gln 2540 2545 2550 Trp Phe Val Glu Arg Asn Met Val Ile Pro Glu Gly Arg Val Ile 2555 2560 2565 Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Cys Ala Gly 2570 2575 2580 Leu Lys Lys Val Thr Glu Val Arg Gly Tyr Thr Lys Gly Gly Pro 2585 2590 2595 Gly His Glu Glu Pro Val Pro Met Ser Thr Tyr Gly Trp Asn Ile 2600 2605 2610 Val Lys Leu Met Ser Gly Lys Asp Val Phe Tyr Leu Pro Pro Glu 2615 2620 2625 Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Pro Ser 2630 2635 2640 Pro Thr Val Glu Glu Ser Arg Thr Ile Arg Val Leu Lys Met Val 2645 2650 2655 Glu Pro Trp Leu Lys Asn Asn Gln Phe Cys Ile Lys Val Leu Asn 2660 2665 2670 Pro Tyr Met Pro Thr Val Ile Glu His Leu Glu Arg Leu Gln Arg 2675 2680 2685 Lys His Gly Gly Met Leu Val Arg Asn Pro Leu Ser Arg Asn Ser 2690 2695 2700 Thr His Glu Met Tyr Trp Ile Ser Asn Gly Thr Gly Asn Ile Val 2705 2710 2715 Ser Ser Val Asn Met Val Ser Arg Leu Leu Leu Asn Arg Phe Thr 2720 2725 2730 Met Thr His Arg Arg Pro Thr Ile Glu Lys Asp Val Asp Leu Gly 2735 2740 2745 Ala Gly Thr Arg His Val Asn Ala Glu Pro Glu Thr Pro Asn Met 2750 2755 2760 Asp Val Ile Gly Glu Arg Ile Lys Arg Ile Lys Glu Glu His Ser 2765 2770 2775 Ser Thr Trp His Tyr Asp Asp Glu Asn Pro Tyr Lys Thr Trp Ala 2780 2785 2790 Tyr His Gly Ser Tyr Glu Val Lys Ala Thr Gly Ser Ala Ser Ser 2795 2800 2805 Met Ile Asn Gly Val Val Lys Leu Leu Thr Lys Pro Trp Asp Val 2810 2815 2820 Val Pro Met Val Thr Gln Met Ala Met Thr Asp Thr Thr Pro Phe 2825 2830 2835 Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg Thr Pro 2840 2845 2850 Arg Pro Met Pro Gly Thr Arg Lys Val Met Glu Ile Thr Ala Glu 2855 2860 2865 Trp Leu Trp Arg Thr Leu Gly Arg Asn Lys Arg Pro Arg Leu Cys 2870 2875 2880 Thr Arg Glu Glu Phe Thr Lys Lys Val Arg Thr Asn Ala Ala Met 2885 2890 2895 Gly Ala Val Phe Thr Glu Glu Asn Gln Trp Asp Ser Ala Arg Ala 2900 2905 2910 Ala Val Glu Asp Glu Glu Phe Trp Lys Leu Val Asp Arg Glu Arg 2915 2920 2925 Glu Leu His Lys Leu Gly Lys Cys Gly Ser Cys Val Tyr Asn Met 2930 2935 2940 Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Lys Ala Lys 2945 2950 2955 Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg Tyr Leu 2960 2965 2970 Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp Phe Ser 2975 2980 2985 Arg Glu Asn Ser Tyr Ser Gly Val Glu Gly Glu Gly Leu His Lys 2990 2995 3000 Leu Gly Tyr Ile Leu Arg Asp Ile Ser Lys Ile Pro Gly Gly Ala 3005 3010 3015 Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile Thr Glu 3020 3025 3030 Asp Asp Leu His Asn Glu Glu Lys Ile Thr Gln Gln Met Asp Pro 3035 3040 3045 Glu His Arg Gln Leu Ala Asn Ala Ile Phe Lys Leu Thr Tyr Gln 3050 3055 3060 Asn Lys Val Val Lys Val Gln Arg Pro Thr Pro Lys Gly Thr Val 3065 3070 3075 Met Asp Ile Ile Ser Arg Lys Asp Gln Arg Gly Ser Gly Gln Val 3080 3085 3090 Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Ala Gln Leu 3095 3100 3105 Ile Arg Gln Met Glu Gly Glu Gly Val Leu Ser Lys Ala Asp Leu 3110 3115 3120 Glu Asn Pro His Pro Leu Glu Lys Lys Ile Thr Gln Trp Leu Glu 3125 3130 3135 Thr Lys Gly Val Glu Arg Leu Lys Arg Met Ala Ile Ser Gly Asp 3140 3145 3150 Asp Cys Val Val Lys Pro Ile Asp Asp Arg Phe Ala Asn Ala Leu 3155 3160 3165 Leu Ala Leu Asn Asp Met Gly Lys Val Arg Lys Asp Ile Pro Gln 3170 3175 3180 Trp Gln Pro Ser Lys Gly Trp His Asp Trp Gln Gln Val Pro Phe 3185 3190 3195 Cys Ser His His Phe His Glu Leu Ile Met Lys Asp Gly Arg Lys 3200 3205 3210 Leu Val Val Pro Cys Arg Pro Gln Asp Glu Leu Ile Gly Arg Ala 3215 3220 3225 Arg Ile Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala Cys 3230 3235 3240 Leu Gly Lys Ala Tyr Ala Gln Met Trp Ala Leu Met Tyr Phe His 3245 3250 3255 Arg Arg Asp Leu Arg Leu Ala Ser Asn Ala Ile Cys Ser Ala Val 3260 3265 3270 Pro Val His Trp Val Pro Thr Ser Arg Thr Thr Trp Ser Ile His 3275 3280 3285 Ala His His Gln Trp Met Thr Thr Glu Asp Met Leu Thr Val Trp 3290 3295 3300 Asn Arg Val Trp Ile Glu Asp Asn Pro Trp Met Glu Asp Lys Thr 3305 3310 3315 Pro Val Thr Thr Trp Glu Asp Val Pro Tyr Leu Gly Lys Arg Glu 3320 3325 3330 Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ser Arg Ala Thr 3335 3340 3345 Trp Ala Gln Asn Ile Leu Thr Ala Ile Gln Gln Val Arg Ser Leu 3350 3355 3360 Ile Gly Asn Glu Glu Phe Leu Asp Tyr Met Pro Ser Met Lys Arg 3365 3370 3375 Phe Arg Lys Glu Glu Glu Ser Glu Gly Ala Ile Trp 3380 3385 3390 <210> 16 <211> 3387 <212> PRT <213> Dengue virus type 4 <220> <221> VARIANT <222> (521)..(521) <223> Xaa stands for Val or Leu <220> <221> VARIANT <222> (1396)..(1396) <223> Xaa stands for Ser or Leu <220> <221> VARIANT <222> (1506)..(1506) <223> Xaa stands for Gly or Trp <400> 16 Met Asn Gln Arg Lys Lys Val Val Arg Pro Pro Phe Asn Met Leu Lys 1 5 10 15 Arg Glu Arg Asn Arg Val Ser Thr Pro Gln Gly Leu Val Lys Arg Phe 20 25 30 Ser Thr Gly Leu Phe Ser Gly Lys Gly Pro Leu Arg Met Val Leu Ala 35 40 45 Phe Ile Thr Phe Leu Arg Val Leu Ser Ile Pro Pro Thr Ala Gly Ile 50 55 60 Leu Lys Arg Trp Gly Gln Leu Lys Lys Asn Lys Ala Ile Arg Ile Leu 65 70 75 80 Ile Gly Phe Arg Lys Glu Ile Gly Arg Met Leu Asn Ile Leu Asn Gly 85 90 95 Arg Lys Arg Ser Thr Ile Thr Leu Leu Cys Leu Ile Pro Thr Val Met 100 105 110 Ala Phe His Leu Ser Thr Arg Asp Gly Glu Pro Leu Met Ile Val Ala 115 120 125 Lys His Glu Arg Gly Arg Pro Leu Leu Phe Lys Thr Thr Glu Gly Ile 130 135 140 Asn Lys Cys Thr Leu Ile Ala Met Asp Leu Gly Glu Met Cys Glu Asp 145 150 155 160 Thr Val Thr Tyr Lys Cys Pro Leu Leu Val Asn Thr Glu Pro Glu Asp 165 170 175 Ile Asp Cys Trp Cys Asn Leu Thr Ser Thr Trp Val Met Tyr Gly Thr 180 185 190 Cys Thr Gln Ser Gly Glu Arg Arg Arg Glu Lys Arg Ser Val Ala Leu 195 200 205 Thr Pro His Ser Gly Met Gly Leu Glu Thr Arg Ala Glu Thr Trp Met 210 215 220 Ser Ser Glu Gly Ala Trp Lys His Ala Gln Arg Val Glu Ser Trp Ile 225 230 235 240 Leu Arg Asn Pro Gly Phe Ala Leu Leu Ala Gly Phe Met Ala Tyr Met 245 250 255 Ile Gly Gln Thr Gly Ile Gln Arg Thr Val Phe Phe Val Leu Met Met 260 265 270 Leu Val Ala Pro Ser Tyr Gly Met Arg Cys Val Gly Val Gly Asn Arg 275 280 285 Asp Phe Val Glu Gly Val Ser Gly Gly Ala Trp Val Asp Leu Val Leu 290 295 300 Glu His Gly Gly Cys Val Thr Thr Met Ala Gln Gly Lys Pro Thr Leu 305 310 315 320 Asp Phe Glu Leu Thr Lys Thr Thr Ala Lys Glu Val Ala Leu Leu Arg 325 330 335 Thr Tyr Cys Ile Glu Ala Ser Ile Ser Asn Ile Thr Thr Ala Thr Arg 340 345 350 Cys Pro Thr Gln Gly Glu Pro Tyr Leu Lys Glu Glu Gln Asp Gln Gln 355 360 365 Tyr Ile Cys Arg Arg Asp Val Val Asp Arg Gly Trp Gly Asn Gly Cys 370 375 380 Gly Leu Phe Gly Lys Gly Gly Val Val Thr Cys Ala Lys Phe Ser Cys 385 390 395 400 Ser Gly Lys Ile Thr Gly Asn Leu Val Gln Ile Glu Asn Leu Glu Tyr 405 410 415 Thr Val Val Val Thr Val His Asn Gly Asp Thr His Ala Val Gly Asn 420 425 430 Asp Thr Ser Asn His Gly Val Thr Ala Thr Ile Thr Pro Arg Ser Pro 435 440 445 Ser Val Glu Val Lys Leu Pro Asp Tyr Gly Glu Leu Thr Leu Asp Cys 450 455 460 Glu Pro Arg Ser Gly Ile Asp Phe Asn Glu Met Ile Leu Met Lys Met 465 470 475 480 Lys Lys Lys Thr Trp Leu Val His Lys Gln Trp Phe Leu Asp Leu Pro 485 490 495 Leu Pro Trp Thr Ala Gly Ala Asp Thr Ser Glu Val His Trp Asn Tyr 500 505 510 Lys Glu Arg Met Val Thr Phe Lys Xaa Pro His Ala Lys Arg Gln Asp 515 520 525 Val Thr Val Leu Gly Ser Gln Glu Gly Ala Met His Ser Ala Leu Ala 530 535 540 Gly Ala Thr Glu Val Asp Ser Gly Asp Gly Asn His Met Phe Ala Gly 545 550 555 560 His Leu Lys Cys Lys Val Arg Met Glu Lys Leu Arg Ile Lys Gly Met 565 570 575 Ser Tyr Thr Met Cys Ser Gly Lys Phe Ser Ile Asp Lys Glu Met Ala 580 585 590 Glu Thr Gln His Gly Thr Thr Val Val Lys Val Lys Tyr Glu Gly Ala 595 600 605 Gly Ala Pro Cys Lys Val Pro Ile Glu Ile Arg Asp Val Asn Lys Glu 610 615 620 Lys Val Val Gly Arg Ile Ile Ser Ser Thr Pro Leu Ala Glu Asn Thr 625 630 635 640 Asn Ser Ala Thr Asn Ile Glu Leu Glu Pro Pro Phe Gly Asp Ser Tyr 645 650 655 Ile Val Ile Gly Val Gly Asn Ser Ala Leu Thr Leu His Trp Phe Arg 660 665 670 Lys Gly Ser Ser Ile Gly Lys Met Phe Glu Ser Thr Tyr Arg Gly Ala 675 680 685 Lys Arg Met Ala Ile Leu Gly Glu Thr Ala Trp Asp Phe Gly Ser Val 690 695 700 Gly Gly Leu Phe Thr Ser Leu Gly Lys Ala Val His Gln Val Phe Gly 705 710 715 720 Ser Val Tyr Thr Thr Met Phe Gly Gly Val Ser Trp Met Ile Arg Ile 725 730 735 Leu Ile Gly Phe Leu Val Leu Trp Ile Gly Thr Asn Ser Arg Asn Thr 740 745 750 Ser Met Ala Met Thr Cys Ile Ala Val Gly Gly Ile Thr Leu Phe Leu 755 760 765 Gly Phe Thr Val Gln Ala Asp Met Gly Cys Val Val Ser Trp Ser Gly 770 775 780 Lys Glu Leu Lys Cys Gly Ser Gly Ile Phe Val Val Asp Asn Val His 785 790 795 800 Thr Trp Thr Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ala Arg Leu 805 810 815 Ala Ser Ala Ile Leu Asn Ala His Lys Asp Gly Val Cys Gly Ile Arg 820 825 830 Ser Thr Thr Arg Leu Glu Asn Val Met Trp Lys Gln Ile Thr Asn Glu 835 840 845 Leu Asn Tyr Val Leu Trp Glu Gly Gly His Asp Leu Thr Val Val Ala 850 855 860 Gly Asp Val Lys Gly Val Leu Thr Lys Gly Lys Arg Ala Leu Thr Pro 865 870 875 880 Pro Val Asn Asp Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala Lys 885 890 895 Ile Phe Thr Pro Glu Ala Arg Asn Ser Thr Phe Leu Ile Asp Gly Pro 900 905 910 Asp Thr Ser Glu Cys Pro Asn Glu Arg Arg Ala Trp Asn Phe Leu Glu 915 920 925 Val Glu Asp Tyr Gly Phe Gly Met Phe Thr Thr Asn Ile Trp Met Lys 930 935 940 Phe Arg Glu Gly Ser Ser Glu Val Cys Asp His Arg Leu Met Ser Ala 945 950 955 960 Ala Ile Lys Asp Gln Lys Ala Val His Ala Asp Met Gly Tyr Trp Ile 965 970 975 Glu Ser Ser Lys Asn Gln Thr Trp Gln Ile Glu Lys Ala Ser Leu Ile 980 985 990 Glu Val Lys Thr Cys Leu Trp Pro Lys Thr His Thr Leu Trp Ser Asn 995 1000 1005 Gly Val Leu Glu Ser Gln Met Leu Ile Pro Lys Ser Tyr Ala Gly 1010 1015 1020 Pro Phe Ser His His Asn Tyr Arg Gln Gly Tyr Ala Thr Gln Thr 1025 1030 1035 Val Gly Pro Trp His Leu Gly Lys Leu Glu Ile Asp Phe Gly Glu 1040 1045 1050 Cys Pro Gly Thr Thr Val Ala Ile Gln Glu Asp Cys Asp His Arg 1055 1060 1065 Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys Leu Val Thr 1070 1075 1080 Gln Trp Cys Cys Arg Ser Cys Thr Met Pro Pro Leu Arg Phe Leu 1085 1090 1095 Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu Ser 1100 1105 1110 Glu Lys Glu Glu Asn Met Val Lys Ser Gln Val Thr Ala Gly Gln 1115 1120 1125 Gly Thr Ser Glu Thr Phe Ser Met Gly Leu Leu Cys Leu Thr Leu 1130 1135 1140 Phe Val Glu Glu Cys Leu Arg Arg Arg Val Thr Arg Lys His Met 1145 1150 1155 Ile Leu Val Val Val Ile Thr Leu Cys Ala Ile Ile Leu Gly Gly 1160 1165 1170 Leu Thr Trp Met Asp Leu Leu Arg Ala Leu Ile Met Leu Gly Asp 1175 1180 1185 Thr Met Ser Gly Arg Ile Gly Gly Gln Ile His Leu Ala Ile Met 1190 1195 1200 Ala Val Phe Lys Met Ser Pro Gly Tyr Val Leu Gly Val Phe Leu 1205 1210 1215 Arg Lys Leu Thr Ser Arg Glu Thr Ala Leu Met Val Ile Gly Met 1220 1225 1230 Ala Met Thr Thr Val Leu Ser Ile Pro His Asp Leu Met Glu Leu 1235 1240 1245 Ile Asp Gly Ile Ser Leu Gly Leu Ile Leu Leu Lys Ile Val Thr 1250 1255 1260 His Phe Asp Asn Thr Gln Val Gly Thr Leu Ala Leu Ser Leu Thr 1265 1270 1275 Phe Ile Arg Ser Thr Met Pro Leu Val Met Ala Trp Arg Thr Ile 1280 1285 1290 Met Ala Val Leu Phe Val Val Thr Leu Ile Pro Leu Cys Arg Thr 1295 1300 1305 Ser Cys Leu Gln Lys Gln Ser His Trp Val Glu Ile Thr Ala Leu 1310 1315 1320 Ile Leu Gly Ala Gln Ala Leu Pro Val Tyr Leu Met Thr Leu Met 1325 1330 1335 Lys Gly Ala Ser Arg Arg Ser Trp Pro Leu Asn Glu Gly Ile Met 1340 1345 1350 Ala Val Gly Leu Val Ser Leu Leu Gly Ser Ala Leu Leu Lys Asn 1355 1360 1365 Asp Val Pro Leu Ala Gly Pro Met Val Ala Gly Gly Leu Leu Leu 1370 1375 1380 Ala Ala Tyr Val Met Ser Gly Ser Ser Ala Asp Leu Xaa Leu Glu 1385 1390 1395 Lys Ala Ala Asn Val Gln Trp Asp Glu Met Ala Asp Ile Thr Gly 1400 1405 1410 Ser Ser Pro Ile Ile Glu Val Lys Gln Asp Glu Asp Gly Ser Phe 1415 1420 1425 Ser Ile Arg Asp Val Glu Glu Thr Asn Met Ile Thr Leu Leu Val 1430 1435 1440 Lys Leu Ala Leu Ile Thr Val Ser Gly Leu Tyr Pro Leu Ala Ile 1445 1450 1455 Pro Val Thr Met Thr Leu Trp Tyr Met Trp Gln Val Lys Thr Gln 1460 1465 1470 Arg Ser Gly Ala Leu Trp Asp Val Pro Ser Pro Ala Ala Thr Gln 1475 1480 1485 Lys Ala Ala Leu Ser Glu Gly Val Tyr Arg Ile Met Gln Arg Gly 1490 1495 1500 Leu Phe Xaa Lys Thr Gln Val Gly Val Gly Ile His Met Glu Gly 1505 1510 1515 Val Phe His Thr Met Trp His Val Thr Arg Gly Ser Val Ile Cys 1520 1525 1530 His Glu Thr Gly Arg Leu Glu Pro Ser Trp Ala Asp Val Arg Asn 1535 1540 1545 Asp Met Ile Ser Tyr Gly Gly Gly Trp Arg Leu Gly Asp Lys Trp 1550 1555 1560 Asp Lys Glu Glu Asp Val Gln Val Leu Ala Ile Glu Pro Gly Lys 1565 1570 1575 Asn Pro Lys His Val Gln Thr Lys Pro Gly Leu Phe Lys Thr Leu 1580 1585 1590 Thr Gly Glu Ile Gly Ala Val Thr Leu Asp Phe Lys Pro Gly Thr 1595 1600 1605 Ser Gly Ser Pro Ile Ile Asn Arg Lys Gly Lys Val Ile Gly Leu 1610 1615 1620 Tyr Gly Asn Gly Val Val Thr Lys Ser Gly Asp Tyr Val Ser Ala 1625 1630 1635 Ile Thr Gln Ala Glu Arg Ile Gly Glu Pro Asp Tyr Glu Val Asp 1640 1645 1650 Glu Asp Ile Phe Arg Lys Lys Arg Leu Thr Ile Met Asp Leu His 1655 1660 1665 Pro Gly Ala Gly Lys Thr Lys Arg Ile Leu Pro Ser Ile Val Arg 1670 1675 1680 Glu Ala Leu Lys Arg Arg Leu Arg Thr Leu Ile Leu Ala Pro Thr 1685 1690 1695 Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu Pro 1700 1705 1710 Ile Arg Tyr Gln Thr Pro Ala Val Lys Ser Glu His Thr Gly Arg 1715 1720 1725 Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Thr Arg Leu 1730 1735 1740 Leu Ser Ser Thr Arg Val Pro Asn Tyr Asn Leu Ile Val Met Asp 1745 1750 1755 Glu Ala His Phe Thr Asp Pro Ser Ser Val Ala Ala Arg Gly Tyr 1760 1765 1770 Ile Ser Thr Arg Val Glu Met Gly Glu Ala Ala Ala Ile Phe Met 1775 1780 1785 Thr Ala Thr Pro Pro Gly Ala Thr Asp Pro Phe Pro Gln Ser Asn 1790 1795 1800 Ser Pro Ile Glu Asp Ile Glu Arg Glu Ile Pro Glu Arg Ser Trp 1805 1810 1815 Asn Thr Gly Phe Asp Trp Ile Thr Asp Tyr Gln Gly Lys Thr Val 1820 1825 1830 Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Asn Cys 1835 1840 1845 Leu Arg Lys Ser Gly Lys Lys Val Ile Gln Leu Ser Arg Lys Thr 1850 1855 1860 Phe Asp Thr Glu Tyr Pro Lys Thr Lys Leu Thr Asp Trp Asp Phe 1865 1870 1875 Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Arg Ala 1880 1885 1890 Gly Arg Val Ile Asp Pro Arg Arg Cys Leu Lys Pro Val Ile Leu 1895 1900 1905 Thr Asp Gly Pro Glu Arg Val Ile Leu Ala Gly Pro Ile Pro Val 1910 1915 1920 Thr Pro Ala Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg Asn 1925 1930 1935 Pro Ala Gln Glu Asp Asp Gln Tyr Val Phe Ser Gly Asp Pro Leu 1940 1945 1950 Lys Asn Asp Glu Asp His Ala His Trp Thr Glu Ala Lys Met Leu 1955 1960 1965 Leu Asp Asn Ile Tyr Thr Pro Glu Gly Ile Ile Pro Thr Leu Phe 1970 1975 1980 Gly Pro Glu Arg Glu Lys Thr Gln Ala Ile Asp Gly Glu Phe Arg 1985 1990 1995 Leu Arg Gly Glu Gln Arg Lys Thr Phe Val Glu Leu Met Arg Arg 2000 2005 2010 Gly Asp Leu Pro Val Trp Leu Ser Tyr Lys Val Ala Ser Ala Gly 2015 2020 2025 Ile Ser Tyr Lys Asp Arg Glu Trp Cys Phe Thr Gly Glu Arg Asn 2030 2035 2040 Asn Gln Ile Leu Glu Glu Asn Met Glu Val Glu Ile Trp Thr Arg 2045 2050 2055 Glu Gly Glu Lys Lys Lys Leu Arg Pro Arg Trp Leu Asp Ala Arg 2060 2065 2070 Val Tyr Ala Asp Pro Met Ala Leu Lys Asp Phe Lys Glu Phe Ala 2075 2080 2085 Ser Gly Arg Lys Ser Ile Thr Leu Asp Ile Leu Thr Glu Ile Ala 2090 2095 2100 Ser Leu Pro Thr Tyr Leu Ser Ser Arg Ala Lys Leu Ala Leu Asp 2105 2110 2115 Asn Ile Val Met Leu His Thr Thr Glu Arg Gly Gly Arg Ala Tyr 2120 2125 2130 Gln His Ala Leu Asn Glu Leu Pro Glu Ser Leu Glu Thr Leu Met 2135 2140 2145 Leu Val Ala Leu Leu Gly Ala Met Thr Ala Gly Ile Phe Leu Phe 2150 2155 2160 Phe Met Gln Gly Lys Gly Ile Gly Lys Leu Ser Met Gly Leu Ile 2165 2170 2175 Thr Ile Ala Val Ala Ser Gly Leu Phe Trp Val Ala Glu Ile Gln 2180 2185 2190 Pro Gln Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe Leu Met 2195 2200 2205 Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln Asp 2210 2215 2220 Asn Gln Leu Ile Tyr Val Ile Leu Thr Ile Leu Thr Ile Ile Gly 2225 2230 2235 Leu Ile Ala Ala Asn Glu Met Gly Leu Ile Glu Lys Thr Lys Thr 2240 2245 2250 Asp Phe Gly Phe Tyr Gln Val Lys Thr Glu Thr Thr Ile Leu Asp 2255 2260 2265 Val Asp Leu Arg Pro Ala Ser Ala Trp Thr Leu Tyr Ala Val Ala 2270 2275 2280 Thr Thr Ile Leu Thr Pro Met Leu Arg His Thr Ile Glu Asn Thr 2285 2290 2295 Ser Ala Asn Leu Ser Leu Ala Ala Ile Ala Asn Gln Ala Ala Val 2300 2305 2310 Leu Met Gly Leu Gly Lys Gly Trp Pro Leu His Arg Met Asp Leu 2315 2320 2325 Gly Val Pro Leu Leu Ala Met Gly Cys Tyr Ser Gln Val Asn Pro 2330 2335 2340 Thr Thr Leu Thr Ala Ser Leu Val Met Leu Ser Val His Tyr Ala 2345 2350 2355 Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr Arg Glu Ala Gln 2360 2365 2370 Lys Arg Thr Ala Ala Gly Ile Met Lys Asn Pro Thr Val Asp Gly 2375 2380 2385 Ile Thr Val Ile Asp Leu Glu Pro Ile Ser Tyr Asp Pro Lys Phe 2390 2395 2400 Glu Lys Gln Leu Gly Gln Val Met Leu Leu Val Leu Cys Ala Gly 2405 2410 2415 Gln Leu Leu Leu Met Arg Thr Thr Trp Ala Phe Cys Glu Val Leu 2420 2425 2430 Thr Leu Ala Thr Gly Pro Ile Leu Thr Leu Trp Glu Gly Asn Pro 2435 2440 2445 Gly Arg Phe Trp Asn Thr Thr Ile Ala Val Ser Thr Ala Asn Ile 2450 2455 2460 Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu Ala Phe Ser Leu 2465 2470 2475 Ile Lys Asn Val Gln Thr Pro Arg Arg Gly Thr Gly Thr Thr Gly 2480 2485 2490 Glu Thr Leu Gly Glu Lys Trp Lys Arg Gln Leu Asn Ser Leu Asp 2495 2500 2505 Arg Lys Glu Phe Glu Glu Tyr Lys Arg Ser Gly Ile Leu Glu Val 2510 2515 2520 Asp Arg Thr Glu Ala Lys Ser Ala Leu Lys Asp Gly Ser Lys Ile 2525 2530 2535 Lys His Ala Val Ser Arg Gly Ser Ser Lys Ile Arg Trp Ile Val 2540 2545 2550 Glu Arg Gly Met Val Lys Pro Lys Gly Lys Val Val Asp Leu Gly 2555 2560 2565 Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Met Ala Thr Leu Lys Asn 2570 2575 2580 Val Thr Glu Val Lys Gly Tyr Thr Lys Gly Gly Pro Gly His Glu 2585 2590 2595 Glu Pro Ile Pro Met Ala Thr Tyr Gly Trp Asn Leu Val Lys Leu 2600 2605 2610 His Ser Gly Val Asp Val Phe Tyr Lys Pro Thr Glu Gln Val Asp 2615 2620 2625 Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Ser Asn Pro Thr Ile 2630 2635 2640 Glu Glu Gly Arg Thr Leu Arg Val Leu Lys Met Val Glu Pro Trp 2645 2650 2655 Leu Ser Ser Lys Pro Glu Phe Cys Ile Lys Val Leu Asn Pro Tyr 2660 2665 2670 Met Pro Thr Val Ile Glu Glu Leu Glu Lys Leu Gln Arg Lys His 2675 2680 2685 Gly Gly Asn Leu Val Arg Cys Pro Leu Ser Arg Asn Ser Thr His 2690 2695 2700 Glu Met Tyr Trp Val Ser Gly Ala Ser Gly Asn Ile Val Ser Ser 2705 2710 2715 Val Asn Thr Thr Ser Lys Met Leu Leu Asn Arg Phe Thr Thr Arg 2720 2725 2730 His Arg Lys Pro Thr Tyr Glu Lys Asp Val Asp Leu Gly Ala Gly 2735 2740 2745 Thr Arg Ser Val Ser Thr Glu Thr Glu Lys Pro Asp Met Thr Ile 2750 2755 2760 Ile Gly Arg Arg Leu Gln Arg Leu Gln Glu Glu His Lys Glu Thr 2765 2770 2775 Trp His Tyr Asp Gln Glu Asn Pro Tyr Arg Thr Trp Ala Tyr His 2780 2785 2790 Gly Ser Tyr Glu Ala Pro Ser Thr Gly Ser Ala Ser Ser Met Val 2795 2800 2805 Asn Gly Val Val Lys Leu Leu Thr Lys Pro Trp Asp Val Val Pro 2810 2815 2820 Met Val Thr Gln Leu Ala Met Thr Asp Thr Thr Pro Phe Gly Gln 2825 2830 2835 Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg Thr Pro Gln Pro 2840 2845 2850 Lys Pro Gly Thr Arg Met Val Met Thr Thr Thr Ala Asn Trp Leu 2855 2860 2865 Trp Ala Leu Leu Gly Lys Lys Lys Asn Pro Arg Leu Cys Thr Arg 2870 2875 2880 Glu Glu Phe Ile Ser Lys Val Arg Ser Asn Ala Ala Ile Gly Ala 2885 2890 2895 Val Phe Gln Glu Glu Gln Gly Trp Thr Ser Ala Ser Glu Ala Val 2900 2905 2910 Asn Asp Ser Arg Phe Trp Glu Leu Val Asp Lys Glu Arg Ala Leu 2915 2920 2925 His Gln Glu Gly Lys Cys Glu Ser Cys Val Tyr Asn Met Met Gly 2930 2935 2940 Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Arg Ala Lys Gly Ser 2945 2950 2955 Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg Phe Leu Glu Phe 2960 2965 2970 Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp Phe Gly Arg Glu 2975 2980 2985 Asn Ser Trp Ser Gly Val Glu Gly Glu Gly Leu His Arg Leu Gly 2990 2995 3000 Tyr Ile Leu Glu Glu Ile Asp Lys Lys Asp Gly Asp Leu Met Tyr 3005 3010 3015 Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile Thr Glu Asp Asp 3020 3025 3030 Leu Gln Asn Glu Glu Leu Ile Thr Glu Gln Met Ala Pro His His 3035 3040 3045 Lys Ile Leu Ala Lys Ala Ile Phe Lys Leu Thr Tyr Gln Asn Lys 3050 3055 3060 Val Val Lys Val Leu Arg Pro Thr Pro Arg Gly Ala Val Met Asp 3065 3070 3075 Ile Ile Ser Arg Lys Asp Gln Arg Gly Ser Gly Gln Val Gly Thr 3080 3085 3090 Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Val Gln Leu Ile Arg 3095 3100 3105 Gln Met Glu Ala Glu Gly Val Ile Thr Gln Asp Asp Met Gln Asn 3110 3115 3120 Pro Lys Gly Leu Lys Glu Arg Val Glu Lys Trp Leu Lys Glu Cys 3125 3130 3135 Gly Val Asp Arg Leu Lys Arg Met Ala Ile Ser Gly Asp Asp Cys 3140 3145 3150 Val Val Lys Pro Leu Asp Glu Arg Phe Gly Thr Ser Leu Leu Phe 3155 3160 3165 Leu Asn Asp Met Gly Lys Val Arg Lys Asp Ile Pro Gln Trp Glu 3170 3175 3180 Pro Ser Lys Gly Trp Lys Asn Trp Gln Glu Val Pro Phe Cys Ser 3185 3190 3195 His His Phe His Lys Ile Phe Met Lys Asp Gly Arg Ser Leu Val 3200 3205 3210 Val Pro Cys Arg Asn Gln Asp Glu Leu Ile Gly Arg Ala Arg Ile 3215 3220 3225 Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala Cys Leu Gly 3230 3235 3240 Lys Ala Tyr Ala Gln Met Trp Ser Leu Met Tyr Phe His Arg Arg 3245 3250 3255 Asp Leu Arg Leu Ala Ser Met Ala Ile Cys Ser Ala Val Pro Thr 3260 3265 3270 Glu Trp Phe Pro Thr Ser Arg Thr Thr Trp Ser Ile His Ala His 3275 3280 3285 His Gln Trp Met Thr Thr Glu Asp Met Leu Lys Val Trp Asn Arg 3290 3295 3300 Val Trp Ile Glu Asp Asn Pro Asn Met Thr Asp Lys Thr Pro Val 3305 3310 3315 His Ser Trp Glu Asp Ile Pro Tyr Leu Gly Lys Arg Glu Asp Leu 3320 3325 3330 Trp Cys Gly Ser Leu Ile Gly Leu Ser Ser Arg Ala Thr Trp Ala 3335 3340 3345 Lys Asn Ile His Thr Ala Ile Thr Gln Val Arg Asn Leu Ile Gly 3350 3355 3360 Lys Glu Glu Tyr Val Asp Tyr Met Pro Val Met Lys Arg Tyr Ser 3365 3370 3375 Ala Pro Ser Glu Ser Glu Gly Val Leu 3380 3385 <210> 17 <211> 10176 <212> DNA <213> Dengue virus type 1 <400> 17 atgaacaacc aacgaaaaaa gacggctcga ccgtctttca atatgctgaa acgcgcgaga 60 aaccgcgtgt caactgtttc acagttggcg aagagattct caaaaggatt gctctcaggc 120 caaggaccca tgaaattggt gatggctttc atagcattcc taagatttct agccataccc 180 ccaacagcag gaattttggc tagatggggc tcattcaaga agaatggagc gatcaaagtg 240 ctacggggtt tcaagaaaga aatctcaaac atgttgaata taatgaatag aaggaaaaga 300 tctgtgacca tgctccttat gctgatgcct acagccttgg cgttccattt gaccacacga 360 gggggagagc cgcacatgat agtcagcaag caggaaagag gaaagtcact tttgtttaag 420 acctcagcag gtgtcaacat gtgcaccctt atagcgatgg atttgggaga gttatgtgag 480 gacacaatga cttacaaatg ccctcgaatc actgaggcgg aaccagatga cgttgattgt 540 tggtgcaatg ccacagacac atgggtgacc tatggaacat gttcccaaac tggcragcac 600 cgacgagaca aacgttccgt cgcactggcc ccacacgtgg gacttggttt ggaaacaaga 660 actgaaacgt ggatgtcctc tgaaggcgct tggaaacaga tacaaagagt ggagacttgg 720 gccctgagac acccaggatt cacggtgata gccctttttc tagcacatgc cataggaaca 780 tccatcaccc agaaagggat tattttcatt ttgttaatgc tggtaacacc atccatggcc 840 atgcgatgcg tgggaatagg cagcagggac ttcgtggaag gactgtcagg agcaacttgg 900 gtagatgtgg tactggaaca tggaagttgc gtcaccacca tggcaaaaga caaaccaaca 960 ttggacattg aactcttgaa gacggaagtc acaaaccctg ccgtcctgcg caaactgtgc 1020 attgaagcta aaatatcaaa caccaccacc gactcaagat gtccaacaca aggagaagcc 1080 acactggtgg aagaacaaga cgcgaacttt gtgtgtcgac gaacgtttgt ggacagaggc 1140 tggggcaatg gctgtgggct cttcggaaaa ggaagcctac taacgtgtgc taagttcaag 1200 tgtgtgacaa aactggaagg aaagatagtt caatatgaaa acttgaaata ttcagtaata 1260 gtcaccgtcc acactggaga ccagcatcag gtgggaaatg aaagcacaga acatgggaca 1320 actgcaacta taacacctca agctcctacg acggaaatac agctgaccga ctacggagct 1380 cttacattgg attgttcacc tagaacagga ctagacttta atgaaatggt gttgttgaca 1440 atgaaagaaa aatcatggct agtccacaaa caatggtttc tagacttacc actgccttgg 1500 acctcgggag cctcaacatc acaagagact tggaacagac aagatttgct ggtgacattt 1560 aagacagctc atgcaaagaa gcaggaagta gtcgtactag gatcacaaga aggagcaatg 1620 cacactgcgt tgaccggagc gacagaaatc caaacgtctg gaacgacaac aatttttgca 1680 ggacacttga aatgtagact aaagatggac aaactgactc taaaagggat gtcatatgtg 1740 atgtgcacag gctcattcaa gctagagaaa gaagtggctg agacccagca tggaaccgtt 1800 ctagtgcaga ttaaatacga aggaacagat gcaccatgca agatcccttt ttcgacccaa 1860 gatgaaaaag gagtaaccca gaatgggaga ttgataacag ccaaccccat agtcactgac 1920 aaagaaaaac cagtcaacat tgaggcagaa ccgccttttg gtgagagtta catcgtgata 1980 ggagcaggtg aaaaagcttt gaaactaagc tggttcaaga aaggaagcag catagggaaa 2040 atgtttgagg caactgccag aggagcacga aggatggcca tactgggaga caccgcatgg 2100 gactttggtt ctataggagg agtgttcacg tctgttggaa aattagtaca ccagattttt 2160 ggaactgcat atggagtttt gttcagcggt gtttcctgga ccatgaaaat aggaataggg 2220 gttctgctga catggctagg attaaactca aggaacacgt ccctctcgat gacgtgcatt 2280 gcagttggcc tagtaacact atacctagga gtcatggttc aggcggattc aggatgcgta 2340 attaattgga aaggtagaga actcaaatgt ggaagtggca tttttgtcac caatgaagtt 2400 cacacttgga cagagcaata caaatttcaa gctgactccc caaagagact atcagcagcc 2460 atcgggaagg catgggagga aggtgtgtgt ggaattcgat cagcaactcg tctcgagaac 2520 atcatgtgga agcaaatatc aaatgaactg aaccacatct tacttgaaaa tgacatgaaa 2580 ttcacagtgg ttgtaggaga tgttgctggg atcttggctc aaggaaagaa aatgattagg 2640 ccacaaccca tggaacacaa atactcgtgg aaaagctggg gaaaggctaa aatcataggg 2700 gcagatgtac agaactccac cttcatcatt gacggcccaa acaccccaga atgccctgat 2760 gaccaaagag catggaacat ttgggaagtt gaggactatg gatttggaat tttcacgaca 2820 aacatatggc tgaaattgcg tgactcctac acccaattgt gtgaccaccg gctaatgtca 2880 gctgccatca aggacagcaa ggcagttcac gctgacatgg ggtactggat agaaagtgaa 2940 aagaacgaga cctggaagct ggcaagagcc tcgttcatag aagttaaaac atgtrtttgg 3000 ccaaaatccc acactctatg gagcaacgga gttctggaaa gtgaaatgat aattccaaag 3060 atctatggag gaccaatatc tcagcataac tacagaccag gatatttcac acaaacggta 3120 gggccgtggc acctaggcaa gttggaactg gattttgact tgtgtgaggg taccacagtc 3180 gttgtggatg aacattgtgg aaatcgagga ccatctctta ggaccacaac agtcacagga 3240 aagataattc atgaatggtg ttgtagatct tgtacgctac cacccttacg tttcagagga 3300 gaagatgggt gctggtacgg tatggaaatc agaccagtca aggaaaagga agagaatcta 3360 gtcaaatcaa tggtctctgc agggtcaggg gaagtggaca gcttttcact aggactgcta 3420 tgcatatcaa taatgatcga agaggtaatg agatccagat ggagtagaaa aatgctgatg 3480 actggaacac tggctgtgtt cctccttctc ataatgggac aattgacatg gaatgatctg 3540 atcagattat gcatcatggt tggagccaac gcttccgata gaatggggat gggaacgacg 3600 tacctagctc tgatggccac ttttaaaatg agaccaatgt ttgctgtagg gctattattc 3660 cgcagactaa catccagaga agttcttctt ctaacaattg gattgagtct agtggcatct 3720 gtggagttac caaattcctt ggaggagctg ggggatggac ttgcaatggg cattatgatt 3780 ttaaaattat tgactgactt tcaatcacat cagttgtggg ctaccttgct gtccttgaca 3840 tttatcaaaa caacgttttc cttgcactat gcatggaaga caatggctat ggtactgtca 3900 attgtatctc tcttcccctt atgcctgtcc acgacctccc agaaaacaac atggcttccg 3960 gtgctattgg gatctcttgg atgcaaacca ctaaccatgt ttcttatagc agaaaacaaa 4020 atctggggaa ggaaaagttg gcccctcaat gaagggatca tggctgttgg aatagtcagc 4080 atcctactaa gttcactcct caaaaatgat gtgccgctag ctgggccact aatagctgga 4140 ggcatgctaa tagcatgtta tgttatatct ggaagctcag ccgacctatc actagagaaa 4200 gcagctgagg tctcctggga agaagaagca gaacactctg gtgcctcaca caacatatta 4260 gtggaggtcc aagatgatgg aaccatgaag ataaaggatg aagagagaga tgacacgcta 4320 accattctcc ttaaagcaac tctgctagca gtttcagggg tgtacccatt atcaatacca 4380 gcgacccttt ttgtgtggta cttttggcag aaaaagaaac agagatctgg agtgttatgg 4440 gacacaccta gccctccaga agtggaaaga gcagttcttg atgatggtat ctatagaatt 4500 atgcagagag gactgttggg caggtcccaa gtaggggtag gagtttttca agaaaacgtg 4560 ttccacacaa tgtggcatgt caccagggga gctgtactca tgtatcaagg gaagagactg 4620 gaaccgagct gggccagtgt caaaaaagac ctgatctcat atggaggagg ttggaggctt 4680 caaggatcct ggaacacggg agaagaagtg caggtgattg ctgttgaacc agggaaaaac 4740 cccaaaaatg tacaaacagc gccgggcacc tttaagaccc ctgaaggtga agttggagcc 4800 attgccctag attttaaacc cggcacatct ggatccccca tcgtgaacag agaaggaaaa 4860 atagtaggtc tttatggaaa tggagtagtg acaacaagtg gaacctacgt yagtgccata 4920 gctcaagcca aagcatcaca agaagggccc ctaccagaga ttgaagacga agtgtttagg 4980 aaaagaaact taacaattat ggacctacat ccaggatcgg ggaaaacaag aagatatctt 5040 ccagccatag tccgtgaggc cataaaaagg aagctgcgca cactaattct ggctcccaca 5100 agggttgtcg cttccgaaat ggcagaagcg ctcaagggaa tgccaataag gtaccaaaca 5160 acagcagtga agagtgaaca cacaggaaaa gagatagttg acctcatgtg tcacgccact 5220 ttcaccatgc gcctcctgtc tcccgtgaga gttcccaatt acaacatgat tatcatggat 5280 gaagcacatt tcactgatcc atccagcata gcggccagag ggtacatctc aacccgagtg 5340 ggcatgggtg aagcagctgc gattttcatg acagccactc ccccaggatc agtggaggcc 5400 tttccacaga gcaacgcagt tatccaagat gaggaaagag acattcctga gagatcatgg 5460 aactcaggct atgagtggat cactgacttc ccaggtaaaa cagtctggtt tgttccaagc 5520 attaaagcag gaaatgacat tgccaactgc ttaagaaaga atggaaaacg ggtgattcaa 5580 ttgagcagga aaacctttga tacagagtac caaaaaacaa aaaataacga ctgggattat 5640 gtcgtcacaa cagacatctc cgaaatggga gcaaatttcc gggccgacag ggtgatagac 5700 ccaagacggt gtctgaaacc ggtaatacta aaagatggtc cagagcgtgt cattctagca 5760 ggaccgatgc cagtgactgt ggccagtgcc gcccagagga gaggaagaat tggaaggaac 5820 cacaataagg aaggtgatca gtacatctac atgggacagc ctttaaacaa cgatgaagat 5880 cacgctcact ggacagaagc aaaaatgctc cttgataata taaacacacc agaagggatt 5940 atcccagccc tcttcgagcc ggagagagaa aagagtgcag caatagacgg ggaatacaga 6000 ctgcggggtg aagcaaggaa aacgtttgtg gagctcatga gaagaggaga tctacctgtc 6060 tggctatcct acaaagttgc ctcagaaggc ttccagtact ctgacagaag atggtgcttt 6120 gacggggaaa ggaacaacca ggtgttggag gagaacatgg acgtggagat ttggacaaaa 6180 gaaggagaaa gaaagaaact acgaccccgc tggctggatg ccagaacata ctcagaccca 6240 ctggccctgc gcgagtttaa agagtttgca gcaggaagaa gaagcgtctc aggtgatcta 6300 atactagaaa tagggaaact tccacaacac ttgacgcaaa gggcccagaa tgccttggac 6360 aacctggtca tgttgcacaa ctccgaacaa ggaggaagag cctatagaca tgcaatggaa 6420 gaactgccag acaccataga aacgttgatg ctcctagctt tgatagctgt gttaactggt 6480 ggagtgacgc tgttcttcct atcaggaaag ggcctaggga aaacatctat cggcctactc 6540 tgcgtgatgg cttcaagcgt actgctatgg atggccagtg tggagcccca ttggatagcg 6600 gcctccatca tactggagtt cttcctgatg gtgttgctta ttccagagcc agacagacaa 6660 cgcactccgc aggacaacca gctggcatat gtggtgatag gtttgttatt catgatactg 6720 acagtagcag ccaatgagat gggactgctg gaaaccacaa agaaagactt agggattggc 6780 catgtggctg ttgaaaatca ccaccatgcc gcaatgctgg acgtagactt acatccagct 6840 tcagcctgga ccctctatgc agtggccaca acaattatca ctcccatgat gaggcacaca 6900 atcgaaaaca caacggcaaa catttccctg acagccattg caaaccaggc agctatattg 6960 atgggacttg acaaaggatg gccaatatcg aagatggaca taggagttcc acttctcgcc 7020 ttggggtgct attcccaggt gaatccactg acgctgayag cagcggtgtt gatgctagyg 7080 gctcattacg ccataattgg acctggactg caagcaaaag ctactagaga agctcaaaaa 7140 aggacagcgg ccggaataat gaaaaatcca accattgatg gaattgttgc aatagatttg 7200 gaccctgtgg tttatgatgc aaaatttgag aaacaactag gccaaataat gttgttgata 7260 ctatgcacat cacagatcct cttgatgcgg actacatggg ccttgtgcga atccatcaca 7320 ctggccactg gacctctgac cacgctttgg gagggatctc caggaaaatt ttggaacacc 7380 acgatagcgg tttccatggc aaacattttc agaggaagtt atctagcagg agcaggtctg 7440 gccttctcat taatgaaatc tctaggagga ggtaggagag gcacaggagc ccaaggggaa 7500 acactgggag agaaatggaa aagacagctg aaccaactga gcaagtcaga attcaacacc 7560 tataaaagga gtgggattat ggaagtggac agatccgaag ccaaagaggg actgaaaaga 7620 ggagaaacaa ccaaacatgc agtgtcgaga ggaaccgcta aactgaggtg gtttgtggag 7680 aggaaccttg tgaaaccaga agggaaagtc atagacctcg gttgtggaag aggtggctgg 7740 tcatactatt gcgctgggct gaagaaagtc acagaagtga agggatatac aaaaggagga 7800 cctggacatg aggaaccaat cccaatggcg acctatggat ggaacctagt aaaactgcac 7860 tccgggaaag acgtattctt tataccacct gagaaatgtg ataccctttt gtgtgatatt 7920 ggtgagtcct ctccaaaccc aactatagag gaaggaagaa cgctacgcgt tctaaagatg 7980 gtggaaccat ggctcagagg aaaccaattt tgcataaaaa ttctgaatcc ctacatgcca 8040 agtgtggtgg aaactctgga gcaaatgcaa agaaaacatg gaggaatgct agtgcgaaat 8100 ccactttcaa gaaattctac tcatgaaatg tattgggttt catgtggaac aggaaacatt 8160 gtgtcagcag taaacatgac atccagaatg ttgctaaatc gattcacaat ggcccacagg 8220 aaaccaacat atgaaagaga cgtggaccta ggcgccggaa caagacatgt ggcagtggaa 8280 ccagaggtag ccaacctaga tatcattggc cagaggatag agaacataaa acatgaacat 8340 aagtcaacat ggcattatga tgaggacaat ccatacaaaa catgggccta tcatggatca 8400 tatgaggtca agccatcagg atcagcctca tccatggtca atggcgtggt gaaactgctc 8460 accaaaccat gggatgtcat ccccatggtc acacaaatag ccatgactga cactacaccc 8520 tttggacaac agagggtgtt taaagagaaa gttgacacac gcacaccaaa agcaaaacga 8580 ggcacagcac aaatcatgga ggtgacagcc aggtggttat ggggttttct ttctagaaac 8640 aagaaaccaa gaatttgtac aagagaggag ttcacaagaa aagttaggtc aaacgcagcc 8700 attggagcag tgttcgttga tgaaaatcaa tggaactcag caaaagaagc agtggaagat 8760 gaccggttct gggaccttgt gcacagagag agggagcttc acaaacaggg aaaatgtgcc 8820 acgtgtgttt acaacatgat ggggaagaga gagaaaaagc taggagagtt cggaaaggca 8880 aaaggaagtc gtgcaatatg gtacatgtgg ttaggagcac gctttctaga gttcgaagct 8940 cttggtttca tgaacgaaga tcactggttc agcagagaga attcactcag cggagtggaa 9000 ggagaaggac ttcacaaact tggatatata ctcagagaca tatcaaagat tccaggggga 9060 aacatgtatg cagatgacac agccggatgg gatacaagga taacagagga tgacctccag 9120 aatgaggcca aaattactga catcatggaa cccgaacatg ccctactggc taagtcaatc 9180 ttcaagctaa cctaccaaaa taaggtggta agggttcaga gaccagcaaa aaatggaacc 9240 gtgatggatg tcatatccag acgtgaccag agaggaagtg gccaggttgg aacttatggc 9300 ttaaacactt tcaccaacat ggaagcccag ctgataagac aaatggagtc tgagggaatc 9360 ttttcaccca acgaattaga gaccccaaat ttagccgaga gagttctcga ctggctggaa 9420 aaatatggcg tcgaaaggct gaaaagaatg gcaatcagtg gagatgactg cgtagtgaaa 9480 ccaattgatg acaggttcgc gacagcctta acagctctga atgatatggg aaaagtaaga 9540 aaagatatac cacaatggga accttcaaaa ggatggaatg attggcaaca agtgcctttt 9600 tgttcacacc acttccacca gctgattatg aaggatggga gggaaatagt ggtgccatgc 9660 cgcaaccaag atgaacttgt gggtagggct agagtatcac aaggtgctgg atggagcctg 9720 agagaaactg catgcctagg caagtcatat gcacaaatgt ggcagctgat gtacttccac 9780 aggagagacc tgagactagc cgctaatgct atctgttcag ccgttccagt tgattgggtc 9840 ccaaccagcc gcaccacctg gtcgatccat gcccatcacc aatggatgac aacagaagac 9900 atgctgtcag tatggaatag ggtttggata gaggaaaacc catggatgga ggacaaaacc 9960 cgtatatcca gttgggaaga tgttccatat ctaggaaaaa gggaagatca atggtgtgga 10020 tctctgatag gcttaacagc aagggccacc tgggccacca acatacaagt ggccataaac 10080 caagtgagaa gactaattgg gaatgagaat tattcagatt acatgacatc aatgaagaga 10140 ttcaagaacg agagtgaacc cgaaggggca ctctgg 10176 <210> 18 <211> 10173 <212> DNA <213> Dengue virus type 2 <400> 18 atgaataacc aacggaaaaa ggcgaaaaac acgcctttca atatgctgaa acgcgagaga 60 aaccgcgtgt cgactgtgca acagctgaca aagagattct cacttggaat gctgcaggga 120 cgaggaccat taaaactgtt catggccctg gtagctttcc ttcgtttcct aacaatccca 180 ccaacagcag ggatattgaa gagatgggga acaattaaaa aatcaaaagc cattaatgtt 240 ttgagagggt tcaggaaaga gatcggaagg atgctgaaca tcttgaatag gagacgcagg 300 tctgcaggca tgatcattat gctgattcca acagtgatgg cgttccatct aaccacacgc 360 aacggagaac cacacatgat cgtcggtata caggagaaag ggaaaagtct tctgttcaaa 420 acagaggatg gtgtgaacat gtgcaccctc atggctatgg accttggtga attgtgtgaa 480 gacacaatca cgtacaaatg tcctcttctc aggcagaatg agccagaaga catagactgt 540 tggtgcaact ccacgtccac gtgggtaacc tatgggacct gtaccactac gggagaacat 600 agaagagaaa aaagatcagt ggcactcgtt ccacatgtgg gaatgggact ggagacgcga 660 accgaaacat ggatgtcatc agaaggggct tggaaacatg cccagagaat tgaaacttgg 720 atcctgagac atccaggctt caccataatg gcagcaatcc tggcatacac catagggacg 780 acacatttcc agagagtcct gattttcatt ctactgacag ctgtcgctcc ttcaatgaca 840 atgcgttgca taggaatatc aaatagagac tttgtagaag gggtttcagg aggaagttgg 900 gttgacatag tcttagaaca tggaagctgt gtgacgacga tggcaaaaaa caaaccaaca 960 ttggattttg aactgataaa aacggaagcc aaacagcctg ccaccctaag gaagtactgc 1020 atagaagcaa agctaaccaa cacaacaaca gaatcccgtt gcccaacaca aggggaaccc 1080 agcctaaaag aagagcagga taagaggttc gtctgcaaac actccatggt agacagagga 1140 tggggaaatg gatgtgggtt atttggaaag ggaggcattg tgacctgtgc tawgttcaca 1200 tgcaaaaaga acatggaagg gaaaatcgtg caaccagaaa acttggaata caccattgtg 1260 gtaacacctc actcagggga ggagcatgcg gtcggaaatg acacaggaaa acacggcaag 1320 gaaatcaaag taacaccaca gagttccatc acagaagctg aattgacagg ttatggcacc 1380 gtcacgatgg agtgctcccc gagaacaggc ctcgacttca atgagatggt gttgctgcag 1440 atggaaaata ragcttggct ggtgcatagg caatggtttc tagacctgcc attaccatgg 1500 ctgcccggag cggataaaca agagtcaaat tggatacaga aagaaacatt ggtcactttc 1560 aaaaatcccc atgcgaagaa acaggatgtt gttgttttag gatcccaaga aggggccatg 1620 catacagcac tcacaggagc cacagaaatc caaatgtcgt caggaaactt gctcttcact 1680 ggacatctca agtgcaggct gagaatggac aagctacagc ttaaaggaat gtcatactcc 1740 atgtgcacag gaaagtttaa agttgtgaag gaaatagcag aaacacaaca tggaacgata 1800 gtcatcagag tgcaatatga aggggacggc tctccatgta aaatcccttt tgagataatg 1860 gatttggaaa aaagatatgt cttaggccgc ctgatcacag tcaacccaat tgtaacagaa 1920 aaagatagcc cagtcaacat agaagcagaa cctccattcg gagacagcta catcatcata 1980 ggagtagagc cgggacaact gaagctcaac tggttcaaga aaggaagttc tatcggccaa 2040 atgtttgaga caacgatgag aggggcgaag agaatggcca ttttgggtga cacagcctgg 2100 gacttcggat ccctgggagg agtgtttaca tctataggaa aagctctcca ccaagtcttt 2160 ggagcaatct atggagctgc cttcagtggg gtttcatgga ccatgaaaat cctcatagga 2220 gtcattatca catggatagg aatgaactca cgcagcacct cactgtctgt gtcactagta 2280 ctggtgggaa ttgtgacact gtatttggga gtcatggtgc aggccgatag tggttgcgtt 2340 gtgagctgga aaaacaaaga actgaaatgt ggcagtggga ttttcatcac agacaacgtg 2400 cacacatgga cagaacaata caagttccaa ccagaatccc cttcaaaact agcttcagct 2460 atccagaaag ctcaagaaga gggaatttgt gggatccgct cggtaacaag attggagaac 2520 ctaatgtgga aacaaataac accagaattg aatcacattc tagcagaaaa tgaggtgaag 2580 ttgactatca tgacaggaga catcaaagga atcatgcagg caggaaaacg atctttgcgg 2640 cctcagccca ctgagctaaa gtattcatgg aaaacatggg gtaaagcaaa aatgctctct 2700 acagagtctt ataaccaaac ctttctcatt gatggccccg agacggcaga gtgccccaac 2760 acaaatagag cttggaactc gttggaggtt gaagactacg gctttggagt gttcaccacc 2820 aatatatggc taaaattgaa agaaaaacag gatgcattct gcgactcaaa actcatgtcg 2880 gcggccataa aagacaacag agccgtccat gccgacatgg gttactggat agaaagtgca 2940 ctcaatgaca catggaagat agagaaagcc tctttcattg aagtaaaaaa ctgccactgg 3000 ccaaaatcac acaccctctg gagcaatgga gtgctagaaa gtgagatgat aatcccaaag 3060 aatctcgctg gaccagtgtc tcaacacaac tatagaccag gctaccatac acaagtagca 3120 ggaccatggc acctaggtaa gcttgagatg gactttgatt tctgcgatgg aaccacagta 3180 atagtgactg aggactgcgg aaatagagga ccctctttga gaacaactac tgcctctgga 3240 aaactcataa cggaatggtg ctgccgatct tgcacattac cacccctaag atacagaggt 3300 gaggatgggt gctggtacgg gatggaaatc agaccattga aagagaaaga agaaaatctg 3360 gttaactcct tggtcacagc cggacatggg caggttgaca atttttcact gggagtcttg 3420 ggaatggcat tgttcctgga ggaaatgctt aggacccgag taggaacgaa acatgcaata 3480 ctactagttg cagtttcttt tgtgacattg atcacaggga acatgtcctt caaagaccta 3540 ggaagagtgg tggttatggt aggcgctacc atggcggatg acataggtat gggcgtgact 3600 tatcttgccc tactagcagc cttcaaagtt agaccaactt ttgcagctgg actactcttg 3660 agaaagctga cctccaagga attgatgatg actaccatag gaattgtact cctctcccaa 3720 agcactatac cagagaccgt tcttgaattg actgacgcgt tagccttagg catgatggtc 3780 cttaaaatag taagaaacat ggaaaagtat caattagcag tgactatcat ggctatcttg 3840 tgcgtcccaa acgcagtgat attgcaaaat gcatggaaag tgagttgcac aatactggca 3900 gtggtgtccg tttccccact gcttttgaca tcctcacagc aaaaaacgga ttggatacca 3960 ttagcattga caatcaaggg tctcaatcca acagccattt ttctaacaac cctctcaaga 4020 accaacaaga aaagaagctg gccactaaat gaggctatca tggcagtcgg aatggtgagc 4080 attttagcca gttccctcct aaaaaatgat attcccatga caggaccact agtggctgga 4140 gggctcctca ctgtgtgcta cgtgctcact ggacgatcgg ccgacttgga actggagaga 4200 gcaaccgatg tcaaatggga agaccaggca gagatatcag gaagcagtcc aatcctgtca 4260 ataacaatat cagaagatgg tagcatgtcg ataaaaaatg aagaggaaga acaaacactg 4320 accatactca ttagaacagg attgctggtg atctcaggac tttttcctat atcaatacca 4380 gtcacggcag cagcatggta cctgtgggaa gtgaagaaac aacgggccgg agtattgtgg 4440 gacgttcctt cacctccacc catgggaaag gctgaactgg aagatggagc ctacagaatc 4500 aagcaaaaag ggattcttgg gtattctcag atcggagctg gagtttacaa agaaggaaca 4560 tttcatacaa tgtggcatgt cacacgtggc gctgttctaa tgcataaagg aaagaggatt 4620 gaaccttcat gggcggacgt caaaaaagat ctaatatcat atggaggagg ctggaagtta 4680 gaaggagaat ggaaggaagg agaagaagtc caggtgctgg cactggagcc tggaaaaaat 4740 ccaagagccg tccaaacaaa acctggtctt ttcaaaacca acaccggaac aataggtgcc 4800 gtatctctgg acttttcccc tggaacgtca ggatctccaa tcatcgacaa aaaaggaaaa 4860 gttgtgggtc tttatggcaa tggtgttgtc acaaggagtg gagcatatgt gagtgctata 4920 gcccaaactg aaaaaagcat tgaagacaac cccgagatcg aagatgacat tttccgaaag 4980 agaagactga ccatcatgga cctccaccca ggagcgggaa agacgaaaag ataccttcca 5040 gccatagtca gagaagccat aaaacggggt ttgagaacat taatcttggc ccccactaga 5100 gttgtggcag ctgaaatgga ggaagctctt agaggacttc caataagata ccaaacccca 5160 gccatcagag ctgagcacac cgggcgggaa attgtggatc taatgtgtca tgccacattt 5220 accatgaggc tgctgtcacc agttagagtg ccaaactata acttgattat catggacgaa 5280 gcccatttca cagacccagc aagtatagca gctagaggat acatctcaac ccgagtggag 5340 atgggtgagg cagctgggat tttcatgaca gccactcccc cgggaagtag agatccattt 5400 cctcagagca atgcaccaat catagatgaa gaaagagaaa tccctgaacg ttcatggaat 5460 tctggacatg agtgggtcac ggattttaaa gggaagactg tttggttcgt tccaagcata 5520 aaagcaggaa atgatatagc agcttgcctg agaaaaaatg gaaagaaagt aatacaactt 5580 agtaggaaga cttttgattc tgagtatgtc aagactagaa ccaatgattg ggatttcgtg 5640 gttacaactg acatttcaga aatgggtgcc aatttcaaag ctgagagggt tatagacccc 5700 agacgctgca tgaaaccagt catactaaca gatggtgagg agcgggtgat tctggcagga 5760 cctatgccag tgacccactc tagcgcagca caaagaagag ggagaatagg aagaaatcca 5820 aaaaatgaaa atgaccagta catatacatg ggagaacctt tggaaaatga tgaagactgt 5880 gcacactgga aagaagccaa aatgctccta gataacatta acacaccaga aggaatcatt 5940 cctagtatgt ttgaaccaga gcgtgaaaaa gtggatgcca ttgatggcga ataccgtttg 6000 agaggagaag caaggaaaac ctttgtggac ttaatgagga gaggagactt accagtctgg 6060 ttggcttaca aagtggcagc tgaaggcatc aactatgcag acagaaggtg gtgttttgat 6120 ggaattaaga acaaccaaat cctggaagaa aatgtggaag ttgaaatctg gacaaaagaa 6180 ggggaaagga agaaattgaa acccagatgg ttggatgcta ggatctactc tgacccactg 6240 gcgctaaagg aattcaaaga atttgcagcc ggaagaaagt ctctgaccct gaacctaatc 6300 acagaaatgg gtaggcttcc aacattcatg actcagaaga cgagagacgc actggacaac 6360 ctagcagtgc tgcacacggc tgaagcaggt ggaagggcgt acaatcatgc tctcagtgaa 6420 ctgccggaga ccctagagac actgctttta ctgacacttc tggctacagt cacaggaggg 6480 atctttttat tcttgatgag cgggaggggc atagggaaga tgaccctggg aatgtgctgc 6540 ataatcacgg ctagcatcct tctatggtac gcacaratac agccacactg gatagcagct 6600 tcaataatac tggagttttt tctcatagtt ttgcttattc cagaaccaga aaaacagaga 6660 acaccccaag acaaccaatt gacctacgtt gtcatagcca tcctcacagt ggtggccgca 6720 accatggcaa acgagatggg tttcctagaa aaaacgaaga aggatcttgg attaggaagc 6780 attgcaaccc agcaacccga gagcaacatc ctggacatag acctacgtcc cgcatcagct 6840 tggacgctgt atgccgtggc tacaacattc gttacaccaa tgctgagaca tagcattgag 6900 aattcctcag tgaatgtatc cctaacagct atagccaacc aagcaacagt gttaatgggt 6960 ctagggaaag gatggccatt gtcaaagatg gacatcggag ttccccttct cgccattgga 7020 tgttactcac aagtcaatcc cataactctc atagcagctc tcctcttatt ggtagcgcat 7080 tacgccatca ttgggccagg acttcaagca aaagcaacca gagaagccca gaaaagagca 7140 gcggcgggca tcatgaaaaa cccaaccgtc gatggaataa cagtgattga tctagatcca 7200 ataccttatg atccaaagtt ygaaaagcag ttgggacaag taatgctact aatcctctgc 7260 gtgactcaag tattgatgat gaggactaca tgggctctgt gtgaggcctt aaccttagct 7320 acagggccca tctccacact gtgggaagga aatccaggaa ggttctggaa cactaccatt 7380 gcggtgtcaa tggctaacat ctttagaggg agttacctgg ctggagctgg acttctcttt 7440 tccattatga agaacacaac caacacaaga aggggaactg gcaacatagg agagacgctt 7500 ggagagaaat ggaaaagccg attgaacgca ctgggaaaaa gtgaattcca gatctacaag 7560 aaaagtggaa tccaggaagt ggatagaacc ttagcaaaag aaggcatcaa aagaggagaa 7620 acggaccatc acgctgtgtc gcgaggctca gcaaaactga gatggttcgt tgagagaaac 7680 atggtcacac cagaagggaa ggtagtggac ctcggttgtg gcagaggagg ctggtcatac 7740 tattgtggag gactaaaaaa cgtaagagaa gtgaaaggcc taacaaaagg aggaccagga 7800 cacgaagaac ccatccctat gtcaacatat gggtggaatc tggtgcgtct tcagagtgga 7860 gttgacgtct tcttcattcc gccagaaaag tgtgacacat tgttgtgtga cataggggag 7920 tcatcaccaa atcccacagt ggaagcagga cgaacactca gagtccttaa cttagtagaa 7980 aactggttga acaacaacac tcaattttgc ataaaagttc tcaacccata tatgccctca 8040 gttatagaaa aaatggaagc actgcaaagg aaatatggag gagccttagt gagaaatcca 8100 ctctcacgga actccacaca tgagatgtac tgggtatcca atgcttctgg gaacatagtg 8160 tcatcagtga acatgatttc aaggatgttg atcaatagat tcacaatgag acacaagaaa 8220 gccacctacg agccggatgt tgacctcgga agcggaaccc gaaacattgg agttgaaagt 8280 gaaataccaa acctagacat aattgggaaa agaatagaaa aaataaagca agagcatgaa 8340 acatcatggc actacgacca agaccaccca tayaaaacgt gggcatacca tggtagctat 8400 gaaacaaaac agactggatc agcatcatcc atggtcaacg gagtggtcag gctgctgaca 8460 aaaccttggg acgttctccc cacggtgaca cagatggcaa tgacagacac gactccattt 8520 ggacaacagc gtgtttttaa agaaaaagtg gacacgagaa cccaagaacc gaaagaaggc 8580 acgaagaaac taatgagaat cacagcagag tggctgtgga aagaattagg gaagaaaaag 8640 acacctagga tgtgcaccag agaagaattc acaagaaagg tgagaagcaa tgcagccttg 8700 ggggccatat tcactgatga gaacaagtgg aartcggcac gtgaggctgt tgaagatagt 8760 aggttttggg agctggttga caaggaaagg aatctccatc ttgaaggaaa gtgtgagaca 8820 tgtgtgtaca acatgatggg aaaaagagaa aagaagctag gggaattcgg caaggcaaaa 8880 ggcagcagag ccatatggta catgtggctt ggagctcgct tcctggagtt tgaagcccta 8940 ggattcctaa atgaagatca ctggttctct agagagaact ccctgagtgg agtggaagga 9000 gaagggctgc acaagctagg ttacattcta agagacgtga gcaagaaaga gggaggagcc 9060 atgtacgccg acgacacagc aggatgggac acaagaatca cactagaaga cctaaaaaat 9120 gaagaaatgg taacaaacca catggaagga gaacacaaga aattggccga ggctattttc 9180 aaactaacgt atcaaaacaa ggtggtgcgt gtgcaaagac caacaccaag aggcacagta 9240 atggacatca tatcgagaag agaccaaaga ggtagtgggc aagttggtac atatggactc 9300 aatactttca ccaatatgga agcccaatta atcagacaga tggagggaga aggagtcttt 9360 aaaaacatcc agcacctgac agtcacagaa gaagttgccg tgcaaaactg gttggcaaga 9420 gtggggcgcg aaaggttgtc aagaatggcc atcagtggag atgattgtgt tgtgaaacct 9480 ttagatgaca ggttcgcaaa cgctttgaca gctctaaatg acatgggaaa gattagaaaa 9540 gatatacaac aatgggaacc ytcaaaagga tggaacgatt ggacacaagt gcccttctgt 9600 tcacaccatt tccatgagct aatcatgaag gatggtcgtg tacttgttgt tccatgtaga 9660 aaccaagatg aattgattgg cagagcccga atctcccaag gagcagggtg gtctttgcgg 9720 gagacggcct gtttggggaa gtcttacgcc caaatgtgga gcttgatgta tttccacagg 9780 cgtgacctca ggctggcggc aaatgccatt tgctcggcag tgccatcaca ctgggttcca 9840 acaagtcgaa caacctggtc catacacgcc aaacatgaat ggatgacaac ggaagacatg 9900 ctgacagtct ggaacagggt gtggattcga gaaaacccat ggatggaaga caaaacccca 9960 gtggaatcat gggaggaaat cccatacttg ggaaaaagag aagaccaatg gtgcggctca 10020 ttgattgggt taacaagcag ggccacatgg gcaaagaaca tccaagcagc aataaatcag 10080 gttagatctc ttataggcaa tgaggaatac acagattaca tgccatccat gaaaagattt 10140 agaagagaag aggaagaagc aggagtcctg tgg 10173 <210> 19 <211> 10170 <212> DNA <213> Dengue virus type 3 <400> 19 atgaacaacc aacggaaaaa gacgggaaaa ccgtctatca atatgctgaa acgcgtgaga 60 aaccgtgtgt caactggatc acagttggcg aagagattct caagaggatt gctgaacggc 120 caaggaccaa tgaaattggt tatggcattt atagctttcc tcagatttct agccattcca 180 ccgacagcag gagtcttggc tagatggggt acctttaaga agtcgggggc tattaaggtc 240 ttaaaaggct tcaagaagga gatctcaaac atgctgagca ttatcaacaa acggaaaaag 300 acatcgctct gtctcatgat gatgttacca gcaacacttg ctttccactt aacttcacga 360 gatggagagc cgcgcatgat tgtggggaag aatgaaagag gaaaatccct acttttcaag 420 acagcctctg gaatcaacat gtgcacactc atagctatgg atctgggaga gatgtgtgat 480 gacacggtca cttacaaatg cccccacatt accgaagtgg agcctgaaga cattgactgc 540 tggtgcaacc ttacatcgac atgggtgact tatggaacat gcaatcawgc tggagagcat 600 agacgcgata agagatcagt ggcgatagct ccccatgttg gcatgggact ggacacacgc 660 actcaaacct ggatgtcggc tgaaggagct tggagacaag tcgagaaggt agagacatgg 720 gcccttaggc acccagggtt taccatacta gccctatttc ttgcccatta cataggcact 780 tccttgaccc agaaagtggt tatttttata ctattaatgc tggttacccc atccatgaca 840 atgagatgtg taggagtagg aaacagagat tttgtggaag gcctatcggg agctacgtgg 900 gttgacgtgg tgctcgagca cggtgggtgt gtgactacca tggctaagaa caagcccacg 960 ctggacatag agcttcagaa gaccgaggcc acccaactgg cgaccctaag gaagctatgc 1020 attgagggaa aaattaccaa cataacaacc gactcaagat gtcccaccca aggggaagcg 1080 attttacctg aggagcagga ccagaactac gtgtgtaagc atacatacgt ggacagaggc 1140 tggggaaacg gttgtggttt gtttggcaag ggaagcttgg tgacatgcgc gaaatttcaa 1200 tgtttagaat caatagaggg aaaagtggtg caacaygaga acctcaaata caccgtcatc 1260 atcacagtgc acacaggaga ccaacaccag gtgggaaatg aaacgcaggg agtcacggct 1320 gagataacac cccaggcatc aaccgctgaa gccattttac ctgaatatgg aaccctcggg 1380 ctagaatgct caccacggac aggtttggat ttcaatgaaa tgatctcatt gacaatgaag 1440 aacaaagcat ggwtggtaca tagacaatgg ttctttgact tacccctacc atggacatca 1500 ggagctacag cagaaacacc aacttggaac aggaaagagc ttcttgtgac atttaaaaat 1560 gcacatgcaa aaaagcaaga agtagttgtt cttggatcac aagagggagc aatgcataca 1620 gcactgacag gagctacaga gatccaaacc tcaggaggca caagtatctt tgcggggcac 1680 ttaaaatgta gactcaagat ggacaaattg gaactcaagg ggatgagcta tgcaatgtgc 1740 ttragtagct ttgtgttgaa gaaagaagtc tccgaaacgc agcatgggac aatactcatt 1800 aaggttgagt acaaagggga agatgcaccc tgcaagattc ctttctccac ggaggatgga 1860 caaggaaaag ctcacaatgg cagactgatc acagccaatc cagtggtgac caagaaggag 1920 gagcctgtca acattgaggc tgaacctcct tttggagaaa gtaacatagt aattggaatt 1980 ggagacaaag ccctgaaaat caactggtac argaagggaa gctcgattgg gaagatgttc 2040 gaggccactg ccagaggtgc aaggcgcatg gccatcttgg gagacacagc ctgggacttt 2100 ggatcagtgg gtggtgtttt gaattcatta gggaaaatgg tccaccaaat atttgggagt 2160 gcttacacag ccctatttgg tggagtctcc tggatgatga aaattggaat aggtgtcctc 2220 ttaacctgga tagggttgaa ctcaaaaaat acttctatgt cattttcatg catcgcgata 2280 ggaatcatta cactctatct gggagccgtg gtgcaagctg acatggggtg tgtcataaac 2340 tggaaaggca aagaactcaa atgtggaagt ggaattttcg tcactaatga ggtccacacc 2400 tggacagagc aatacaaatt tcaagcagac tcccccaaga gactggcaac agccattgca 2460 ggcgcttggg aaaatggagt gtgcggaatt aggtcaacaa ccagaatgga gaacctcttg 2520 tggaagcaaa tagccaatga actgaattac atattatggg aaaacaacat taaattaacg 2580 gtagttgtag gcgacataac tggggtctta gagcaaggga aaagaacact aacaccacaa 2640 cccatggagc taaaatattc ttggaaaaca tggggaaagg caaaaatagt gacagctgaa 2700 acacaaaatt cctctttcat aatagatggg ccaagcacac cggagtgtcc aagtgcctca 2760 agagcatgga atgtgtggga ggtggaggat tacgggttcg gagttttcac aaccaacata 2820 tggctgaaac tccgagaggt gtacacccaa ctatgtgacc ataggctaat gtcggcagcc 2880 gtcaaggatg agagggctgt acatgccgac atgggctatt ggatagaaag ccaaaagaat 2940 gggagttgga agctagaaaa agcatccttc atagaggtga aaacctgcac atggccaaaa 3000 tcacacactc tctggagcaa tggtgtgcta gagagtgaca tgattatccc aaagagtcta 3060 gctggtccca tttcgcaaca caaccacagg cccgggtacc acacccaaac ggcaggaccc 3120 tggcacttag gaaaattgga gctggacttc aactattgtg aaggaacaac agttgtcatc 3180 tcagaaaact gtgggacaag aggcccatca ttgagaacaa caacggtgtc agggaagttg 3240 atacacgaat ggtgctgccg ctcgtgcaca cttcctcccc tacgatacat gggagaagac 3300 ggctgctggt atggcatgga aatcagaccc attaatgaga aagaagagaa tatggtaaag 3360 tctctagcct cagcagggag tggaaaggtg gacaacttca caatgggtgt cttgtgtttg 3420 gcaatcctct ttgaagaggt gatgagagga aaatttggga aaaaacacat gattgcaggg 3480 gttctcttca cgtttgtgct cctcctctca gggcaaataa catggagaga catggcgcac 3540 acactcataa tgattgggtc caacgcctct gacagaatgg ggatgggcgt cacttaccta 3600 gctctaattg caacatttaa aattcagcca ttcctggctt tgggattctt cctgaggaaa 3660 ctgacatcta gagaaaattt attgctggga gttgggttgg ccatggcagc aacgttacga 3720 ctgccagagg acattgaaca gatggcgaat ggaattgctt tggggctcat ggctcttaaa 3780 ctgataacac aatttgaaac ataccaacta tggacggcat tagtttccct aacgtgttca 3840 aatacaattt tcacgttgac tgttgcctgg agaacagcca ctctgatttt agccggaatt 3900 tcgcttttgc cagtgtgcca gtcttcgagc atgaggaaaa cagattggct cccaatgact 3960 gtggcagcta tgggagctca acccctacca ctttttattt tcagtctgaa agatacactc 4020 aaaaggagaa gctggccact gaatgagggg gtgatggcag ttggacttgt gagcattcta 4080 gctagttctc tccttaggaa tgatgtgcct atggctggac cattagtggc tgggggcttg 4140 ctgatagcgt gctacgtcat aactggcacg tcagcagacc tcactgtaga aaaagcagca 4200 gawgtaacat gggaggaaga ggccgagcaa acaggagtgt cccacaattt aatggtcaca 4260 gttgatgatg atggaacaat gagaataaaa gatgacgaga ctgagaacat cttaacagtg 4320 cttttaaaaa cagcactact aatagtatca ggcatctttc catactccat acccgcaaca 4380 ctgttggtct ggcatacttg gcaaaagcaa acccaaagat ccggcgtcct atgggacgta 4440 cccagccccc cagagacaca gaaagcggaa ctggaagaag gggtctatag gatcaaacag 4500 caaggaattt ttgggaaaac ccaagtgggg gttggagtac agaaagaagg agttttccac 4560 accatgtggc atgtcacaag aggggcagtg ttgacacaca atgggaaaag actggaacca 4620 aactgggcta gcgtgaaaaa agatctgatt tcatacggag gaggatggag attgagtgca 4680 caatggcaaa agggggagga ggtgcaggtt attgccgtag agcctgggaa gaacccaaag 4740 aactttcaaa ccatgccagg catttttcag acaacaacag gggaaatagg agcaattgca 4800 ctggatttca agcctggaac ttcaggatct cccatcataa acagagaggg aaaggtagtg 4860 ggactgtatg gcaatggagt ggttacaaag aatggaggct atgtcagtgg aatagcgcaa 4920 acaaatgcag aaccagatgg accgacacca gagttggaag aagagatgtt caaaaagcga 4980 aatctaacca taatggatct ccatcctggg tcaggaaaga cgcggaaata tcttccagct 5040 attgttagag aggcaatcaa gagacgctta aggactctaa ttttggcacc aacaagggta 5100 gttgcagctg agatggaaga agcattgaaa gggctcccaa taaggtatca aacaactgca 5160 acaaaatctg aacacacagg aagagagatt gttgatctaa tgtgtcacgc aacgttcaca 5220 atgcgcttgc tgtcaccagt cagggttcca aactacaact tgataataat ggatgaggcc 5280 catttcacag acccagccag tatagcggct agagggtaca tatcaactcg tgtaggaatg 5340 ggagaggcag ccgcaatttt catgacagca acaccccctg gaacagctga tgcctttcct 5400 cagagcaacg ctccaattca agatgaagag agagacatac cggaacgctc atggaattca 5460 ggcaatgaat ggattactga ctttgttggg aagacagtgt ggtttgtccc tagcatcaaa 5520 gccggaaatg acatagcaaa ctgcttgcgg aaaaatggaa aaaaggttat tcaactcagc 5580 aggaagacct ttgacacaga atatcaaaag accaaactga atgattggga ctttgtggtg 5640 acaacagaca tttcagaaat gggagccaat ttcaaagcag atagagtgat cgacccaaga 5700 agatgtctca agccggtgat tttgacagat ggacccgagc gggtgatcct ggctggacca 5760 atgccagtca ccgtagcgag cgctgcgcaa aggagaggga gagttggcag gaacccacaa 5820 aaagaaaatg accagtacat attcatgggc cagcctctca acaatgatga agaccatgct 5880 cactggacag aagcaaaaat gctgctggac aacatcaaca caccagaagg gattatacca 5940 gctctctttg aaccagaaag ggagaagtca gccgccatag acggcgaata ccgcctgaag 6000 ggtgagtcca ggaagacttt cgtggaactc atgaggaggg gtgacctccc agtttggcta 6060 gcccataaag tagcatcaga agggatcaaa tatacagata gaaaatggtg ctttgatgga 6120 gaacgtaata atcaaatttt agaggagaat atggatgtgg aaatctggac aaaggaagga 6180 gaaaagaaaa aactgagacc taggtggctt gatgcccgca cttattcaga tcctttagca 6240 ctcaaagaat tcaaggattt tgcagctggc agaaagtcaa tcgcccttga tcttgtgaca 6300 gaaataggaa gagtgccttc acacttagcc cacagaacga gaaacgccct ggataatttg 6360 gtgatgctgc acacgtcaga acatggcggt agggcctaca ggcatgcagt ggaggaacta 6420 ccagaaacga tggaaacact cttactcctg ggactgatga tcttgttaac aggtggagca 6480 atgctcttct tgatatcagg taaagggatt ggaaagactt caataggact catttgtgta 6540 attgcttcca gcggcatgtt atggatggct gatgtcccac tccaatggat cgcatcggct 6600 atagtcctgg agttttttat gatggtgttg ctcataccag aaccagaaaa gcagagaact 6660 ccccaagaca accaactcgc atatgtcgtg ataggcatac ttacattggc tgcaatagta 6720 gcggccaatg aaatgggact gttggaaact acaaagagag atttaggaat gtctaaagaa 6780 ccaggtgttg tttctccaac cagctatttg gatgtggact tgcacccagc atcagcctgg 6840 acattgtacg ccgtggccac aacagtaata acaccaatgt tgagacacac catagagaat 6900 tccacagcaa atgtgtctct ggcagccata gctaaccagg cagtggtcct gatgggttta 6960 gacaaaggat ggccgatatc gaaaatggac ttgggcgtac cactattggc actgggttgc 7020 tattcacaag tgaacccact aactcttgca gcggcagtac ttttgctagt cacacattat 7080 gcaattatag gtccaggatt gcaggcaaaa gccacccgtg aagctcagaa aaggacagct 7140 gctggaataa tgaagaatcc aacggtggat ggaataatga caatagacct agatcctgta 7200 atatatgatt caaaatttga aaagcaacta ggacaggtca tgctcctggt tctgtgtgca 7260 gtccaacttt tattgatgag aacatcatgg gccttgtgtg aagttctaac cctagycaca 7320 ggaccaataa caacactctg ggaaggatca cctgggaagt tctggaacac cacgatagct 7380 gtttccatgg cgaacatctt tagagggagc tatttagcag gagctgggct tgctttttct 7440 atcatgaaat cagttggaac aggaaagaga ggaacagggt cacaaggtga aaccttagga 7500 gaaaagtgga aaaagaaatt aaatcagtta tcccggaaag agtttgacct ttacaagaaa 7560 tccggaatca ccgaagtgga tagaacagaa gccaaagaag ggttaaaaag aggagaaata 7620 acacaccatg ccgtgtccag aggcagcgca aaacttcaat ggttcgtgga gagaaacatg 7680 gtcattcctg aaggaagagt catagaccta ggctgtggaa gaggaggctg gtcatattac 7740 tgtgcaggac tgaaaaaagt tacagaagtg cgaggataca caaaaggcgg cccaggacac 7800 gaagaaccag tacctatgtc tacatacgga tggaacatag tcaagttaat gagtggaaag 7860 gatgtttttt atctgccacc tgaaaagtgt gataccctat tgtgtgacat tggagaatct 7920 tcaccaagcc caacagtgga agaaagcaga accataagag ttttgaagat ggttgaacca 7980 tggctaaaga acaaccagtt ttgcattaaa gtattgaacc catacatgcc aactgtgatt 8040 gagcacttag aaagactaca aaggaaacat ggaggaatgc ttgtgagaaa tccactctca 8100 cgaaactcca cgcacgaaat gtattggata tccaatggta caggcaatat cgtctcttca 8160 gtcaacatgg tatccagatt gctactgaac agattcacaa tgacacacag gagacccacc 8220 atagagaaag atgtggatct aggagcagga acccgacatg tcaatgcgga accagaaaca 8280 cccaacatgg atgtcattgg ggaaagaata aaaaggatca aagaggagca tagttcaaca 8340 tggcactatg atgatgaaaa tccttacaaa acgtgggctt accatggatc ctatgaagta 8400 aaagccacag gctcagcctc ctccatgata aatggagtcg tgaaactcct cacaaaacca 8460 tgggatgtgg tgcccatggt gacacagatg gcaatgacag atacaactcc attcggccag 8520 caaagagttt ttaaagagaa agtggacacc aggacaccta ggcccatgcc aggaacaaga 8580 aaggttatgg agatcacagc ggagtggctt tggaggaccc tgggaaggaa caaaagaccc 8640 agattatgca caagggagga attcacaaag aaggtcagar ccaacgcagc tatgggcgct 8700 gtcttcacag aagagaacca atgggacagt gcgagagctg ctgttgagga cgaagaattt 8760 tggaaacttg tggacagaga acgtgaactc cacaaactgg gcaagtgtgg aagctgcgtt 8820 tacaacatga tgggcaagag agagaaaaaa cttggagagt ttggtaaagc aaaaggcagt 8880 agggctatat ggtacatgtg gttgggagcc aggtaccttg agttcgaggc gctcggattc 8940 ctcaatgaag accactggtt ctcgcgtgaa aactcttaca gtggagtaga aggagaagga 9000 ctgcacaagc tgggatacat cttgagagat atttccaaga tacccggagg agccatgtat 9060 gctgatgaca cagcyggttg ggacacaaga ataacagaag atgacctgca caatgaggaa 9120 aaaatcacac agcagatgga ccctgaacac aggcagctag cgaacgctat attcaagctc 9180 acataccaaa acaaagtggt caaagtccaa cgaccaactc caaagggcac ggtaatggac 9240 atcatatcta ggaaagacca aagaggcagt ggacaggtgg gaacttatgg tctgaacaca 9300 ttcaccaaca tggaagccca gctaatcaga caaatggaag gagaaggcgt gttgtcaaag 9360 gcagacctcg agaaccccca tccgctagag aagaaaatta cacaatggtt ggaaactaaa 9420 ggagtggaaa ggttaaaaag aatggccatc agcggggatg attgcgttgt gaaaccaatc 9480 gacgacagat tcgccaatgc cctgcttgcc ctgaacgata tgggaaaggt tagaaaggac 9540 atacctcaat ggcagccatc aaagggatgg catgattggc aacaggtccc cttctgctcc 9600 caccactttc atgaattgat catgaaagat ggaagaaagt tggtagttcc ctgcagaccc 9660 caggacgaac taataggaag agcgagaatc tcccaaggag caggatggag ccttagagaa 9720 actgcatgtc tagggaaagc ctacgctcaa atgtgggctc tcatgtattt tcacagaaga 9780 gatcttagac tagcatccaa cgccatatgt tcagcagtac cagtccactg ggtccccacg 9840 agcagaacga catggtctat tcatgctcac catcagtgga tgactacaga agacatgctt 9900 actgtctgga acagggtgtg gatagaggac aatccatgga tggaagacaa aactccagtc 9960 acaacgtggg aagatgttcc atatctaggg aagagagaag accaatggtg cggatcactc 10020 ataggtctca cttccagagc aacctgggcc cagaacatac tcacagcaat ccaacaggtg 10080 agaagcctca taggcaatga agagtttctg gactacatgc cttcgatgaa gagattcagg 10140 aaggaggagg agtcagaggg agccatttgg 10170 <210> 20 <211> 10161 <212> DNA <213> Dengue virus type 4 <400> 20 atgaaccaac gaaaaaaggt ggttagacca cctttcaata tgctgaaacg cgagagaaac 60 cgcgtatcaa cccctcaagg gttggtgaag agattctcaa ccggactttt ttctgggaaa 120 ggacccttac ggatggtgct agcattcatc acgtttttgc gagtcctttc catcccacca 180 acagcaggga ttctgaaaag atggggacag ttgaagaaaa ataaggccat caggatactg 240 attggattca ggaaggagat aggccgcatg ctgaacatct tgaacgggag aaaaaggtca 300 acgataacat tgctgtgctt gattcccacc gtaatggcgt ttcacttgtc aacaagagat 360 ggcgaacccc tcatgatagt ggcaaaacat gaaaggggga gacctctctt gtttaagaca 420 acagagggga tcaacaaatg cactctcatt gccatggact tgggtgaaat gtgtgaggac 480 actgtcacgt ataaatgccc cttactggtc aataccgaac ctgaagacat tgattgctgg 540 tgcaatctca cgtctacctg ggtcatgtat gggacatgca cccagagcgg agaacggaga 600 cgagagaagc gctcagtagc tttaacacca cattcaggaa tgggattgga aacaagagct 660 gagacatgga tgtcatcgga aggggcttgg aagcatgctc agagagtaga gagctggata 720 ctcagaaacc caggattcgc gctcttggca ggatttatgg cttatatgat tgggcaaaca 780 ggaatccagc gaactgtctt ctttgtccta atgatgctgg tcgccccatc ctacggaatg 840 cgatgcgtag gagtaggaaa cagagacttt gtggaaggag tctcaggtgg agcatgggtc 900 gatctggtgc tagaacatgg aggatgcgtc acaaccatgg cccagggaaa accaaccytg 960 gattttgaac tgactaagac aacagccaag gaagtggctc tgttaagaac ctattgcatt 1020 gaagcctcaa tatcaaacat aaccacggca acaagatgtc caacgcaagg agagccttat 1080 ctaaaagagg aacaagacca acagtacatt tgccggagag atgtggtaga cagagggtgg 1140 ggcaatggct gtggcttgtt tggaaaagga ggagttgtga catgtgcgaa gttttcatgt 1200 tcggggaaga taacaggcaa tttggtccaa attgagaacc ttgaatacac agtggttgta 1260 acagtccaca atggagacac ccatgcagta ggaaatgaca catccaatca tggagttaca 1320 gccacgataa ctcccaggtc accatcggtg gaagtcaaat tgccggacta tggagaacta 1380 acactcgatt gtgaacccag gtctggaatt gactttaatg agatgattct gatgaaaatg 1440 aaaaagaaaa catggcttgt gcataagcaa tggtttttgg atctacctct accatggaca 1500 gcaggagcag acacatcaga ggttcactgg aattacaaag agagaatggt gacatttaag 1560 gttcctcatg ccaagagaca ggatgtgaca gtgctgggat ctcaggaagg agccatgcat 1620 tctgccctcg ctggagccac agaagtggac tccggtgatg gaaatcacat gtttgcagga 1680 catctcaagt gcaaagtccg tatggagaaa ttgagaatca agggaatgtc atacacgatg 1740 tgttcaggaa agttctcaat tgacaaagag atggcagaaa cacagcatgg gacaacagtg 1800 gtgaaagtca agtatgaagg tgctggagct ccgtgtaaag tccccataga gataagagat 1860 gtgaacaagg aaaaagtggt tgggcgtatc atctcatcca cccctttggc tgagaatacc 1920 aacagtgcaa ccaacataga gttagaaccc ccctttgggg acagctacat agtgataggt 1980 gttggaaaca gtgcattaac actccattgg ttcaggaaag ggagttccat tggcaagatg 2040 tttgagtcca catacagagg tgcaaaacga atggccattc taggtgaaac agcttgggat 2100 tttggttccg ttggtggact gttcacatca ttgggaaagg ctgtgcacca ggtttttgga 2160 agtgtgtata caaccatgtt tggaggagtc tcatggatga ttagaatcct aattgggttc 2220 ctagtgttgt ggattggcac gaactcaagg aacacttcaa tggctatgac gtgcatagct 2280 gttggaggaa tcactctgtt tctgggcttc acagttcaag cagacatggg ttgtgtggtg 2340 tcatggagtg ggaaagaatt gaagtgtgga agcggaattt ttgtggttga caacgtgcac 2400 acttggacag aacagtacaa atttcaaccg gagtccccag cgagactagc gtctgcaata 2460 ttgaatgccc acaaagatgg ggtctgtgga attagatcaa ccacgaggct ggaaaatgtc 2520 atgtggaagc aaataaccaa cgagctaaat tatgttctct gggaaggagg acatgacctc 2580 actgtagtgg ctggggatgt gaagggggtg ttgaccaaag gcaagagagc actcacaccc 2640 ccagtgaatg atctgaaata ttcatggaag acatggggaa aagcaaaaat cttcacccca 2700 gaagcaagaa atagcacatt tttaatagac ggaccagaca cctccgaatg ccccaatgaa 2760 cgaagagcat ggaactttct tgaggtggaa gactatggat ttggcatgtt cacgaccaac 2820 atatggatga aattccgaga aggaagttca gaagtgtgtg accacaggtt aatgtcagcg 2880 gcaattaaag atcagaaagc tgtgcatgct gacatgggtt attggataga gagctcaaaa 2940 aaccagacct ggcagataga gaaagcatct cttattgaag tgaaaacatg tctgtggccc 3000 aagacccaca cattgtggag caatggagtg ctggaaagcc agatgctcat tccaaaatca 3060 tatgcgggcc ctttttcaca gcacaattac cgccagggct atgccacgca aaccgtgggc 3120 ccatggcact taggcaaatt agagatagac tttggagaat gccccggaac aacagtcgca 3180 attcaggagg attgtgacca tagaggccca tctttgagga ccaccactgc atctggaaaa 3240 ctagtcacgc aatggtgctg ccgctcctgc acgatgcctc ccttaaggtt cttgggwgaa 3300 gatgggtgct ggtatgggat ggagattagg cccttgagtg aaaaagaaga gaacatggtc 3360 aaatcacagg taacggccgg acagggcaca tcagaaactt tttctatggg tctgttgtgc 3420 ctgaccttgt ttgtggaaga atgcttgagg agaagagtca ctaggaaaca catgatattg 3480 gttgtggtga tcactctttg tgccatcatc ctaggaggcc tcacatggat ggacttacta 3540 cgagccctca tcatgttggg ggacactatg tctggtagaa taggaggaca gatccaccta 3600 gccatcatgg cagtgttcaa gatgtcacca ggatacgtgc tgggtgtgtt tttaaggaaa 3660 ctcacttcaa gagagacagc actaatggta ataggaatgg ccatgacaac ggtgctttca 3720 attccacatg accttatgga actcattgat ggaatatcac tggggctaat tttgctaaaa 3780 atagtgacac attttgacaa cacccaagtg ggaaccttag ccctttcctt gaccttcata 3840 agatcaacaa tgccattggt catggcttgg aggaccatta tggctgtgtt gtttgtggtc 3900 acactcattc ctttgtgcag gacaagctgt cttcaaaaac agtctcattg ggtagaaata 3960 acagcactca tcctaggagc ccaagctctg ccagtgtacc taatgactct tatgaaagga 4020 gcctcaagaa gatcttggcc tcttaacgag ggcataatgg ctgtgggttt ggttagtctc 4080 ttaggaagcg ctcttttaaa gaatgatgtc cctttagctg gcccaatggt ggcaggaggc 4140 ttacttctgg cggcttacgt gatgagtggt agctcagcag atctgtcact agagaaggcc 4200 gccaatgtgc agtgggatga aatggcagac ataacaggct caagcccaat catagaagtg 4260 aagcaggatg aagatggctc tttctycata cgggacgtcg aggaaaccaa tatgataacc 4320 cttttggtga aactggcact gataacagtg tcaggtctct accccttggc aattccagtc 4380 acaatgacct tatggtacat gtggcaagtg aaaacacaaa gatcaggagc cctgtgggac 4440 gtcccctcac ccgctgccac tcaaaaagcc gcactgtctg aaggagtgta caggatcatg 4500 caaagagggt tatttgggaa aactcaggtt ggagtaggga tacacatgga aggtgtattt 4560 cacacaatgt ggcatgtaac aagaggatca gtgatctgcc atgagactgg gagattggag 4620 ccatcttggg ctgacgtcag gaatgacatg atatcatacg gtgggggatg gagacttgga 4680 gacaaatggg acaaagaaga agatgttcag gtcctcgcca tagaaccagg aaaaaatcct 4740 aaacatgtcc aaacgaaacc cggccttttc aagaccctaa ctggagaaat tggagcagta 4800 acattagatt tcaaacccgg aacgtctggt tctcccatca tcaacaggaa aggaaaagtc 4860 atcggactct atggaaatgg agtagttacc aaatcaggtg attacgtcag tgccataacg 4920 caagccgaaa gaattggaga gccagattat gaagtggatg aggacatttt tcgaaagaaa 4980 agattaacta taatggactt acaccccgga gctggaaaga caaaaagaat tcttccatca 5040 atagtgagag aagccttaaa aaggaggctg cgaaccttga ttttggctcc cacgagagtg 5100 gtggcggccg agatggaaga ggccctacgt ggactgccaa tccgttatca gaccccagct 5160 gtgaaatcag aacacacagg aagagagatt gtagacctca tgtgtcatgc aaccttcaca 5220 acaagacttt tgtcatcaac cagagttcca aattacaacc tcatagtgat ggatgaagca 5280 catttcaccg atccttctag tgtcgcggct agaggataca tctcgaccag ggtggaaatg 5340 ggagaggcag cagccatctt catgaccgca acccctcccg gagcgacaga tccctttccc 5400 cagagcaaca gcccaataga agacatcgag agggaaattc cggaaaggtc atggaacaca 5460 gggttcgact ggataacaga ctaccaaggg aaaactgtgt ggtttgttcc cagcataaaa 5520 gctggaaatg acattgcaaa ttgtttgaga aagtcgggaa agaaagttat ccagttgagt 5580 aggaaaacct ttgatacaga gtatccaaaa acgaaactca cggactggga ttttgtggtc 5640 actacagaca tatctgaaat gggggccaat tttagagctg ggagagtgat agaccctagg 5700 agatgcctca agccagttat cctaacagat gggccagaga gagtcatttt agcaggtcct 5760 attccagtga ctccagcaag cgctgctcag agaagagggc gaataggaag gaacccagca 5820 caagaagacg accaatacgt tttctccgga gacccactaa aaaatgatga agatcatgcc 5880 cactggacag aagcaaagat gctgcttgac aatatctaca ccccagaagg gatcattcca 5940 acattgtttg gtccggaaag ggaaaaaacc caagccattg atggagagtt tcgcctcaga 6000 ggggaacaaa ggaagacttt tgtggaatta atgaggagag gagaccttcc ggtgtggctg 6060 agctataagg tagcttctgc tggcatttct tacaaagatc gggaatggtg cttcacaggg 6120 gaaaggaata accaaatttt agaagaaaac atggaggttg aaatttggac tagagaggga 6180 gaaaagaaaa agctaaggcc aagatggtta gatgcacgtg tatacgctga ccccatggct 6240 ttgaaggatt ttaaggagtt tgctagtgga aggaagagca taactctcga catcctaaca 6300 gagattgcca gtttgccaac ttacctttcc tctagggcca agctcgccct tgataacata 6360 gtcatgctcc acacaacaga aagaggaggg agggcctacc aacacgccct gaacgaactc 6420 ccggagtcac tggaaacact tatgcttgta gctttactag gtgctatgac agcaggtatc 6480 ttcctgtttt tcatgcaagg gaaaggaata gggaaattgt caatgggttt gataaccatt 6540 gcggtggcta gtggcttgct ctgggtagca gaaattcaac cccagtggat agcggcctca 6600 atcatactag agttttttct catggtactg ttgataccgg aaccagaaar acaaaggacc 6660 ccacaagaca atcaattgat ctacgtcata ttgaccattc tcaccattat tggtctcata 6720 gcagccaacg agatggggct gattgaaaaa acaaaaacgg atyttgggtt ttaccaggta 6780 aaaacagaaa ccaccatcct cgatgtggac ttgagaccag cttcagcatg gacgctctat 6840 gcagtagcca ccacaattct gactcccatg ctgagacaca ccatagaaaa cacgtcggcc 6900 aacctatctc tagcagccat tgccaaccag gcggccgtcc taatggggct tggaaaagga 6960 tggccgctcc acagaatgga cctcggtgtg ccgctgttag caatgggatg ctattctcaa 7020 gtgaacccaa yaactttgac agcatcctta gtcatgcttt twgtccatta tgcaataata 7080 ggtccaggat tgcaggcaaa agccacaaga gaggcccaga aaaggacagc tgctgggatc 7140 atgaaaaacc ccacggtgga cgggataaca gtaatagatc tagaaccaat atcctatgac 7200 ccaaaatttg aaaagcaatt agggcaggtc atgctactcg tcttgtgtgc tggacaacta 7260 ctcttgatga gaacaacatg ggctttctgt gaagtcttga ctttggccac aggaccaatc 7320 ttgaccttgt gggagggcaa cccgggaagg ttttggaaca cgaccatagc cgtatccacc 7380 gccaacattt tcaggggaag ttacctggcg ggagctggac tggctttttc actcataaag 7440 aatgtacaaa cccctaggag gggaactggg accacaggag agacactggg agagaagtgg 7500 aagagacagc taaactcatt agacagaaaa gagtttgaag agtataaaag aagtggaata 7560 ctagaagtgg acaggactga agccaagtct gccctgaaag atgggtctaa aatcaagcat 7620 gcagtatcta gagggtccag taagattaga tggattgttg agagagggat ggtaaagcca 7680 aaagggaaag ttgtagatct tggctgtggg agaggaggat ggtcttatta catggcgacg 7740 ctcaagaacg tgactgaagt gaaagggtat acaaaaggag gtccaggaca tgaagaaccg 7800 attcccatgg ctacttatgg ctggaatttg gtcaaactcc attcaggggt tgacgtgttc 7860 tacaaaccca cagagcaagt ggacaccctg ctctgtgata ttggggagtc atcttctaat 7920 ccaacaatag aggaaggaag aacattaaga gttttgaaga tggtggagcc atggctctct 7980 tcaaaacctg aattctgcat caaagtcctt aacccctaca tgccaacagt catagaagag 8040 ctggagaaac tgcagagaaa acatggtggg aaccttgtca gatgcccgct gtccaggaac 8100 tccacccatg agatgtattg ggtgtcagga gcgtcgggaa acattgtgag ctctgtgaac 8160 acaacatcaa agatgttgtt gaacaggttc acaacaaggc ataggaaacc cacttatgag 8220 aaggacgtag atcttggggc aggaacgaga agtgtctcca ctgaaacaga aaaaccagac 8280 atgacaatta ttgggagaag gcttcagcga ttgcaagagg agcacaaaga aacctggcat 8340 tatgatcagg aaaacccata cagaacctgg gcgtatcatg gaagctatga agctccttcg 8400 acaggctctg catcctccat ggtgaacggg gtagtaaaac tgctaacaaa accttgggat 8460 gtggttccaa tggtgaccca gttagccatg acagacacaa ccccttttgg gcaacaaaga 8520 gtgttcaaag agaaggtgga taccagaaca ccacaaccaa aacccggtac acgaatggtt 8580 atgaccacga cagccaattg gctgtgggcc ctccttggga agaagaaaaa wcccagactg 8640 tgcacaaggg aagagttcat ctcaaaagtt agatcaaacg cagccatagg cgcagtcttt 8700 caggaagaac agggatggac atcagccagt gaagctgtga atgacagccg gttttgggaa 8760 ctggttgaca aagaaagggc cctacaccag gaagggaaat gtgaatcgtg tgtctacaac 8820 atgatgggaa aacgtgagaa aaagttagga gagtttggca gagccaaggg aagccgagca 8880 atctggtaca tgtggctggg agcgcggttt ctggaatttg aagccctggg ttttttgaat 8940 gaagatcact ggtttggcag agaaaattca tggagtggag tggaagggga aggtctgcac 9000 agattgggat atatcctgga ggagatagac aagaaggatg gagacctaat gtatgctgat 9060 gacacagcag gctgggacac aagaatcact gaggatgacc ttcaaaatga agaactgatc 9120 acggaacaga tggcccccca ccacaagatc ctagccaaag ccattttcaa actaacctat 9180 caaaacaaag tggtgaaagt cctcagaccc acaccgagag gagcggtgat ggatatcata 9240 tccaggaaag accaaagagg tagtggacaa gttggaacat atggtttgaa cacattcacc 9300 aacatggaag ttcaactcat ccgccaaatg gaagctgaag gagtcatcac acaagatgac 9360 atgcagaacc caaaagggtt gaaagaaaga gttgagaaat ggctgaaaga gtgtggtgtc 9420 gacaggttaa agaggatggc aatcagtgga gacgattgcg tggtgaagcc cctggatgag 9480 aggtttggca cttccctcct cttcttgaac gacatgggaa aggtgaggaa agacattccg 9540 cagtgggaac catctaaggg atggaaaaac tggcaagagg ttcctttttg ctcccaccac 9600 tttcacaaga tcttcatgaa ggatggccgc tcactagttg ttccatgtag aaaccaggat 9660 gaactgatag ggagagccag aatctcgcag ggggctggat ggagcttaag agaaacagcc 9720 tgcctgggca aagcttacgc ccagatgtgg tcgctcatgt acttccacag aagggatctg 9780 cgtttagcct ccatggccat atgctcagca gttccaacgg aatggtttcc aacaagcaga 9840 acaacatggt caatccacgc tcatcatcag tggatgacca ctgaagatat gctcaaagtg 9900 tggaacagag tgtggataga agacaaccct aatatgactg acaagactcc agtccattcg 9960 tgggaagata taccttacct agggaaaaga gaggatttgt ggtgtggatc cctgattgga 10020 ctttcttcca gagccacctg ggcgaagaac attcacacgg ccataaccca ggtcagaaac 10080 ctgatcggaa aagaggaata cgtggattac atgccagtaa tgaaaagata cagcgctcct 10140 tcagagagtg aaggagttct g 10161 <210> 21 <211> 10176 <212> DNA <213> Dengue virus type 1 <400> 21 atgaacaacc aacgaaaaaa gacggctcga ccgtctttca atatgctgaa acgcgcgaga 60 aaccgcgtgt caactgtttc acagttggcg aagagattct caaaaggatt gctctcaggc 120 caaggaccca tgaaattggt gatggctttc atagcattcc taagatttct agccataccc 180 ccaacagcag gaattttggc tagatggggc tcattcaaga agaatggagc gatcaaagtg 240 ctacggggtt tcaagaaaga aatctcaaac atgttgaata taatgaatag aaggaaaaga 300 tctgtgacca tgctccttat gctgatgcct acagccttgg cgttccattt gaccacacga 360 gggggagagc cgcacatgat agtcagcaag caggaaagag gaaagtcact tttgtttaag 420 acctcagcag gtgtcaacat gtgcaccctt atagcgatgg atttgggaga gttatgtgag 480 gacacaatga cttacaaatg ccctcgaatc actgaggcgg aaccagatga cgttgattgt 540 tggtgcaatg ccacagacac atgggtgacc tatggaacat gttcccaaac tggcgagcac 600 cgacgagaca aacgttccgt cgcactggcc ccacacgtgg gacttggttt ggaaacaaga 660 actgaaacgt ggatgtcctc tgaaggcgct tggaaacaga tacaaagagt ggagacttgg 720 gccctgagac acccaggatt cacggtgata gccctttttc tagcacatgc cataggaaca 780 tccatcaccc agaaagggat tattttcatt ttgttaatgc tggtaacacc atccatggcc 840 atgcgatgcg tgggaatagg cagcagggac ttcgtggaag gactgtcagg agcaacttgg 900 gtagatgtgg tactggaaca tggaagttgc gtcaccacca tggcaaaaga caaaccaaca 960 ttggacattg aactcttgaa gacggaagtc acaaaccctg ccgtcctgcg caaactgtgc 1020 attgaagcta aaatatcaaa caccaccacc gactcaagat gtccaacaca aggagaagcc 1080 acactggtgg aagaacaaga cgcgaacttt gtgtgtcgac gaacgtttgt ggacagaggc 1140 tggggcaatg gctgtgggct cttcggaaaa ggaagcctac taacgtgtgc taagttcaag 1200 tgtgtgacaa aactggaagg aaagatagtt caatatgaaa acttgaaata ttcagtaata 1260 gtcaccgtcc acactggaga ccagcatcag gtgggaaatg aaagcacaga acatgggaca 1320 actgcaacta taacacctca agctcctacg acggaaatac agctgaccga ctacggagct 1380 cttacattgg attgttcacc tagaacagga ctagacttta atgaaatggt gttgttgaca 1440 atgaaaaaaa aatcatggct agtccacaaa caatggtttc tagacttacc actgccttgg 1500 acctcgggag cctcaacatc acaagagact tggaacagac aagatttgct ggtgacattt 1560 aagacagctc atgcaaagaa gcaggaagta gtcgtactag gatcacaaga aggagcaatg 1620 cacactgcgt tgaccggagc gacagaaatc caaacgtctg gaacgacaac aatttttgca 1680 ggacacttga aatgtagact aaggatggac aaactgactc taaaagggat gtcatatgtg 1740 atgtgcacag gctcattcaa gctagagaaa gaagtggctg agacccagca tggaaccgtt 1800 ctagtgcaga ttaaatacga aggaacagat gcaccatgca agatcccttt ttcgacccaa 1860 gatgaaaaag gagtaaccca gaatgggaga ttgataacag ccaaccccat agtcactgac 1920 aaagaaaaac cagtcaacat tgaggcagaa ccgccttttg gtgagagtta catcgtgata 1980 ggagcaggtg aaaaagcttt gaaactaagc tggttcaaga aaggaagcag catagggaaa 2040 atgtttgagg caactgccag aggagcacga aggatggcca tactgggaga caccgcatgg 2100 gactttggtt ctataggagg agtgttcacg tctgttggaa aattagtaca ccagattttt 2160 ggaactgcat atggagtttt gttcagcggt gtttcctgga ccatgaaaat aggaataggg 2220 gttctgctga catggctagg attaaactca aggaacacgt ccctctcgat gacgtgcatt 2280 gcagttggcc tagtaacact atacctagga gtcatggttc aggcggattc aggatgcgta 2340 attaattgga aaggtagaga actcaaatgt ggaagtggca tttttgtcac caatgaagtt 2400 cacacttgga cagagcaata caaatttcaa gctgactccc caaagagact atcagcagcc 2460 atcgggaagg catgggagga aggtgtgtgt ggaattcgat cagcaactcg tctcgagaac 2520 atcatgtgga agcaaatatc aaatgaactg aaccacatct tacttgaaaa tgacatgaaa 2580 ttcacagtgg ttgtaggaga tgttgctggg atcttggctc aaggaaagaa aatgattagg 2640 ccacaaccca tggaacacaa atactcgtgg aaaagctggg gaaaggctaa aatcatrggg 2700 gcagatgtac agaactccac cttcatcatt gacggcccaa acaccccaga atgccctgat 2760 gaccaaagag catggaacat ttgggaagtt gaggactatg gatttggaat tttcacgaca 2820 aacatatggc tgaaattgcg tgactcctac acccaattgt gtgaccaccg gctaatgtca 2880 gctgccatca aggacagcaa ggcagttcac gctgacatgg ggtactggat agaaagtgaa 2940 aagaacgaga cctggaagct ggcaagagcc tcgttcatag aagttaaaac atgtatttgg 3000 ccaaaatccc acactctatg gagcaacgga gttctggaaa gtgaaatgat aattccaaag 3060 atctatggag gaccaatatc tcagcataac tacagaccag gatatttcac acaaacggta 3120 gggccgtggc acctaggcaa gttggaactg gattttgact tgtgtgaggg taccacagtc 3180 gttgtggatg aacattgtgg aaatcgagga ccatctctta ggaccacaac agtcacagga 3240 aagataattc atgaatggtg ttgtagatct tgtacgctac cacccttacg tttcagagga 3300 gaagatgggt gctggtacgg tatggaaatc agaccagtca aggaaaagga agagaatcta 3360 gtcaaatcaa tggtctctgc agggtcaggg gaagtggaca gcttttcact aggactgcta 3420 tgcatatcaa taatgatcga agaggtaatg agatccagat ggagtagaaa aatgctgatg 3480 actggaacac tggctgtgtt cctccttctc ataatgggac aattgacatg gaatgatctg 3540 atcagattat gcatcatggt tggagccaac gcttccgata gaatggggat gggaacgacg 3600 tacctagctc tgatggccac ttttaaaatg agaccaatgt ttgctgtagg gctattattc 3660 cgcagactaa catccagaga agttcttctt ctaacaattg gattgagtct agtggcatct 3720 gtggagttac caaattcctt ggaggagctg ggggatggac ttgcaatggg cattatgatt 3780 ttaaaattat tgactgactt tcaatcacat cagttgtggg ctaccttgct gtccttgaca 3840 tttatcaaaa caacgttttc cttgcactat gcatggaaga caatggctat ggtactgtca 3900 attgtatctc tcttcccctt atgcctgtcc acgacctccc agaaaacaac atggcttccg 3960 gtgctattgg gatctcttgg atgcaaacca ctaaccatgt ttcttatagc agaaaacaaa 4020 atctggggaa ggaaaagttg gcccctcaat gaagggatca tggctgttgg aatagtcagc 4080 atcctactaa gttcactcct caagaatgat gtgccgctag ctgggccact aatagctgga 4140 ggcatgctaa tagcatgtta tgttatatct ggaagctcag ccgacctatc actagagaaa 4200 gcagctgagg tctcctggga agaagaagca gaacactctg gtgcctcaca caacatatta 4260 gtggaggtcc aagatgatgg aaccatgaag ataaaggatg aagagagaga tgacacgcta 4320 accattctcc ttaaagcaac tctgctagca gtttcagggg tgtacccatt atcaatacca 4380 gcgacccttt ttgtgtggta cttttggcag aaaaagaaac agagatctgg agtgttatgg 4440 gacacaccta gccctccaga agtggaaaga gcagttcttg atgatggtat ctatagaatt 4500 atgcagagag gactgttggg caggtcccaa gtaggggtag gagtttttca agaaaacgtg 4560 ttccacacaa tgtggcatgt caccagggga gctgtactca tgtatcaagg gaagagactg 4620 gaaccgagct gggccagtgt caaaaaagac ctgatctcat atggaggagg ttggaggctt 4680 caaggatcct ggaacacggg agaagaagtg caggtgattg ctgttgaacc agggaaaaac 4740 cccaaaaatg tacaaacagc gccgggcacc tttaagaccc ctgaaggtga agttggagcc 4800 attgccctag attttaaacc cggcacatct ggatccccca tcgtgaacag agaaggaaaa 4860 atagtaggtc tttatggaaa tggagtagtg acaacaagtg gaacctacgt cagtgccata 4920 gctcaagcca aagcatcaca agaagggccc ctaccagaga ttgaagacga agtgtttagg 4980 aaaagaaaat taacaattat ggacctacat ccaggatcgg ggaaaacaag aagatatctt 5040 ccagccatag tccgtgaggc cataaaaagg aagctgcgca cactaattct ggctcccaca 5100 agggttgtcg cttccgaaat ggcagaagcg ctcaagggaa tgccaataag gtaccaaaca 5160 acagcagtga agagtgaaca cacaggaaaa gagatagttg acctcatgtg tcacgccact 5220 ttcaccatgc gcctcctgtc tcccgtgaga gttcccaatt acaacatgat tatcatggat 5280 gaagcacatt tcactgatcc atccagcata gcggccagag ggtacatctc aacccgagtg 5340 ggcatgggtg aagcagctgc gattttcatg acagccactc ccccaggatc agtggaggcc 5400 tttccacaga gcaacgcagt tatccaagat gaggaaagag acattcctga gagatcatgg 5460 aactcaggct atgagtggat cactgacttc ccaggtaaaa cagtctggtt tgttccaagc 5520 attaaagcag gaaatgacat tgccaactgc ttaagaaaga atggaaaacg ggtgattcaa 5580 ttgagcagga aaacctttga tacagagtac caaaaaacaa aaaataacga ctgggattat 5640 gtcgtcacaa cagacatctc cgaaatggga gcaaatttcc gggccgacag ggtgatagac 5700 ccaagacggt gtctgaaacc ggtaatacta aaagatggtc cagagcgtgt cattctagca 5760 ggaccgatgc cagtgactgt ggccagtgcc gcccagagga gaggaagaat tggaaggaac 5820 cacaataagg aaggtgacca gtacatctac atgggacagc ctttaaacaa cgatgaagat 5880 cacgctcact ggacagaagc aaaaatgctc cttgataata taaacacacc agaagggatt 5940 atcccagccc tcttcgagcc ggagagagaa aagagtgcag caatagacgg ggaatacaga 6000 ctgcggggtg aagcaaggaa aacgtttgtg gagctcatga gaagaggaga tctacctgtc 6060 tggctatcct acaaagttgc ctcagaaggc ttccagtact ctgacagaag atggtgcttt 6120 gacggggaaa ggaacaacca ggtgttggag gagaacatgg acgtggagat ttggacaaaa 6180 gaaggagaaa gaaagaaact acgaccccgc tggctggatg ccagaacata ctcagaccca 6240 ctggccctgc gcgagtttaa agagtttgca gcaggaagaa gaagcgtctc aggtgatcta 6300 atactagaaa tagggaaact tccacaacac ttgacgcaaa gggcccagaa tgccttggac 6360 aacctggtca tgttgcacaa ctccgaacaa ggaggaagag cctatagaca tgcaatggaa 6420 gaactgccag acaccataga aacgttgatg ctcctagctt tgatagctgt gttaactggt 6480 ggagtgacgc tgttcttcct atcaggaaag ggcctaggga aaacatctat cggcctactc 6540 tgcgtgatgg cttcaagcgt actgctatgg atggccagtg tggagcccca ttggatagcg 6600 gcctccatca tactggagtt cttcctgatg gtgttgctta ttccagagcc agacagacaa 6660 cgcactccgc aggacaacca gctggcatat gtggtgatag gtttgttatt catgatactg 6720 acagtagcag ccaatgagat gggactgctg gaaaccacaa agaaagactt agggattggc 6780 catgtggctg ttgaaaatca ccaccatgcc gcaatgctgg acgtagactt acatccagct 6840 tcagcctgga ccctctatgc agtggccaca acaattatca ctcccatgat gaggcacaca 6900 atcgaaaaca caacggcaaa catttccctg acagccattg caaaccaggc agctatattg 6960 atgggacttg acaaaggatg gccaatatcg aagatggaca taggagttcc acttctcgcc 7020 ttggggtgct attcccaggt gaatccaytg acgctgayag cagcggtgtt gatgctagtg 7080 gctcattacg ccataattgg acctggactg caagcaaaag ctactagaga agctcaaaaa 7140 aggacagcgg ccggaataat gaaaaatcca accattgatg gaattgttgc aatagatttg 7200 gaccctgtgg tttatgatgc aaaatttgag aaacaactag gccaaataat gttgttgata 7260 ctatgcacat cacagatcct cttgatgcgg actacatggg ccttgtgcga atccatcaca 7320 ctggccactg gacctctgac cacgctttgg gagggatctc caggaaaatt ttggaacacc 7380 acgatagcgg tttccatggc aaacattttc agaggaagtt atctagcagg agcaggtctg 7440 gccttctcat taatgaaatc tctaggagga ggtaggagag gcacaggagc ccaaggggaa 7500 acactgggag agaaatggaa aagacagctg aaccaactga gcaagtcaga attcaacacc 7560 tataaaagga gtgggattat ggaagtggac agatccgaag ccaaagaggg actgaaaaga 7620 ggagaaacaa ccaaacatgc agtgtcgaga ggaaccgcta aactgaggtg gtttgtggag 7680 aggaaccttg tgaaaccaga agggaaagtc atagacctcg gttgtggaag aggtggctgg 7740 tcatactatt gcgctgggct gaagaaagtc acagaagtga agggatatac aaaaggagga 7800 cctggacatg aggaaccaat cccaatggcg acctatggat ggaacctagt aaaactgcac 7860 tccgggaaag acgtattctt tataccacct gagaaatgtg ataccctttt gtgtgatatt 7920 ggtgagtcct ctccaaaccc aactatagag gaaggaagaa cgctacgcgt tctaaagatg 7980 gtggaaccat ggctcagagg aaaccaattt tgcataaaaa ttctgaatcc ctacatgcca 8040 agtgtggtgg aaactctgga gcaaatgcaa agaaaacatg gaggaatgct agtgcgaaat 8100 ccactttcaa gaaattctac tcatgaaatg tattgggttt catgtggaac aggaaacatt 8160 gtgtcagcag taaacatgac atccagaatg ttgctaaatc gattcacaat ggcccacagg 8220 aaaccaacat atgaaagaga cgtggaccta ggcgccggaa caagacatgt ggcagtggaa 8280 ccagaggtag ccaacctaga tatcattggc cagaggatag agaacataaa acatgaacat 8340 aagtcaacat ggcattatga tgaggacaat ccatataaaa catgggccta tcatggatca 8400 tatgaggtca agccatcagg atcagcctca tccatggtca atggcgtggt gaaactgctc 8460 accaaaccat gggatgtcat ccccatggtc acacaaatag ccatgactga cactacaccc 8520 tttggacaac agagggtgtt taaagagaaa gttgacacac gcacaccaaa agcaaaacga 8580 ggcacagcac aaatcatgga ggtgacagcc aggtggttat ggggttttct ttctagaaac 8640 aagaaaccaa gaatttgtac aagagaggag ttcacaagaa aagttaggtc aaacgcagcc 8700 attggagcag tgttcgttga tgaaaatcaa tggaactcag caaaagaagc agtggaagat 8760 gaccggttct gggaccttgt gcacagagag agggagcttc acaaacaggg aaaatgtgcc 8820 acgtgtgttt acaacatgat ggggaagaga gagaaaaagc taggagagtt cggaaaggca 8880 aaaggaagtc gtgcaatatg gtacatgtgg ttaggagcac gctttctaga gttcgaagct 8940 cttggtttca tgaacgaaga tcactggttc agcagagaga attcactcag cggagtggaa 9000 ggagaaggac ttcacaaact tggatatata ctcagagaca tatcaaagat tccaggggga 9060 aacatgtatg cagatgacac agccggatgg gatacaagga taacagagga tgacctccag 9120 aatgaggcca aaattactga catcatggaa cccgaacatg ccctactggc taagtcaatc 9180 ttcaagctaa cctaccaaaa taaggtggta agggttcaga gaccagcaaa aaatggaacc 9240 gtgatggatg tcatatccag acgtgaccag agaggaagtg gccaggttgg aacttatggc 9300 ttaaacactt tcaccaacat ggaagcccag ctgataagac aaatggagtc tgagggaatc 9360 ttttcaccca acgaattaga gaccccaaat ttagccgaga gagttctcga ctggctggaa 9420 aaatatggcg tcgaaaggct gaaaagaatg gcaatcagtg gagatgactg cgtagtgaaa 9480 ccaattgatg acaggttcgc gacagcctta acagctctga atgatatggg aaaagtaaga 9540 aaagatatac cacaatggga accttcaaaa ggatggaatg attggcaaca agtgcctttt 9600 tgttcacacc acttccacca gctgattatg aaggatggga gggaaatagt ggtgccatgc 9660 cgcaaccaag atgaacttgt gggtagggct agagtatcac aaggtgctgg atggagcctg 9720 agagaaactg catgcctagg caagtcatat gcacaaatgt ggcagctgat gtacttccac 9780 aggagagacc tgagactagc cgctaatgct atctgttcag ccgttccagt tgattgggtc 9840 ccaaccagcc gcaccacctg gtcgatccat gcccatcacc aatggatgac aacagaagac 9900 atgctgtcag tatggaatag ggtttggata gaggaaaacc catggatgga ggacaaaacc 9960 cgtatatcca gttgggaaga tgttccatat ctaggaaaaa gggaagatca atggtgtgga 10020 tctctgatag gcttaacagc aagggccacc tgggccacca acatacaagt ggccataaac 10080 caagtgagaa gactaattgg gaatgagaat tattcagatt acatgacatc aatgaagaga 10140 ttcaagaacg agagtgaacc cgaaggggca ctctgg 10176 <210> 22 <211> 10173 <212> DNA <213> Dengue virus type 2 <400> 22 atgaataacc aacggaaaaa ggcgaaaaac acgcctttca atatgctgaa acgcgagaga 60 aaccgcgtgt cgactgtgca acagctgaca aagagattct cacttggaat gctgcaggga 120 cgaggaccat taaaactgtt catggccctg gtagctttcc ttcgtttcct aacaatccca 180 ccaacagcag ggatattgaa gagatgggga acaattaaaa aatcaaaagc cattaatgtt 240 ttgagagggt tcaggaaaga gatcggaagg atgctgaaca tcttgaatag gagacgcagg 300 tctgcaggca tgatcattat gctgattcca acagtgatgg cgttccatct aaccacacgc 360 aacggagaac cacacatgat cgtcggtata caggagaaag ggaaaagtct tctgttcaaa 420 acagagaatg gtgtgaacat gtgcaccctc atggctatgg accttggtga attgtgtgaa 480 gacacaatca cgtacaaatg tcctcttctc aggcagaatg agccagaaga catagactgt 540 tggtgcaact ccacgtccac gtgggtaacc tatgggacct gtaccactac gggagaacat 600 agaagagaaa aaagatcagt ggcactcgtt ccacatgtgg gaatgggact ggagacgcga 660 accgaaacat ggatgtcatc agaaggggct tggaaacatg cccagagaat tgaaacttgg 720 atcctgagac atccaggctt caccataatg gcagcaatcc tggcatacac catagggacg 780 acacatttcc agagagtcct gattttcatt ctactgacag ctgtcgctcc ttcaatgaca 840 atgcgttgca taggaatatc aaatagagac tttgtagaag gggtttcagg aggaagttgg 900 gttgacatag tcttagaaca tggaagctgt gtgacgacga tggcaaaaaa caaaccaaca 960 ttggattttg aactgataaa aacggaagcc aaacagcctg ccaccctaag gaagtactgc 1020 atagaagcaa agctaaccaa cacaacaaca gaatcccgtt gcccaacaca aggggaaccc 1080 agcctaaaag aagagcagga taagaggttc gtctgcaaac actccatggt agacagagga 1140 tggggaaatg gatgtgggtt atttggaaag ggaggcattg tgacctgtgc tatgttcaaa 1200 tgcaaaaaga acatggaagg gaaaatcgtg caaccagaaa acttggaata caccattgtg 1260 gtaacacctc actcagggga ggagcatgcg gtcggaaatg acacaggaaa acacggcaag 1320 gaaatcaaag taacaccaca gagttccatc acagaagctg aattgacagg ttatggcacc 1380 gtcacgatgg agtgctcccc gagaacaggc ctcgacttca atgagatggt gttgctgcag 1440 atggaaaata aagcttggct ggtgcatagg caatggtttc tagacctgcc attaccatgg 1500 ctgcccggag cggataaaca agagtcaaat tggatacaga aagaaacatt ggtcactttc 1560 aaaaatcccc atgcgaagaa acaggatgtt gttgttttag gatcccaaga aggggccatg 1620 catacagcac tcacaggagc cacagaaatc caaatgtcgt caggaaactt gctcttcact 1680 ggacatctca agtgcaggct gagaatggac aagctacagc ttaaaggaat gtcatactcc 1740 atgtgcacag gaaagtttaa agttgtgaag gaaatagcag aaacacaaca tggaacgata 1800 gtcatcagag tgcaatatga aggggacggc tctccatgta aaatcccttt tgagataatg 1860 gatttggaaa aaagatatgt cttaggccgc ctgatcacag tcaacccaat tgtaacagaa 1920 aaagatagcc cagtcaacat agaagcagaa cctccattcg gagacagcta catcatcata 1980 ggagtagagc cgggacaact gaagctcaac tggttcaaga aaggaagttc tatcggccaa 2040 atgtttgaga caacgatgag aggggcgaag agaatggcca ttttgggtga cacagcctgg 2100 gacttcggat ccctgggagg agtgtttaca tctataggaa aagctctcca ccaagtcttt 2160 ggagcaatct atggagctgc cttcagtggg gtttcatgga ccatgaaaat cctcatagga 2220 gtcattatca catggatagg aatgaactca cgcagcacct cactgtctgt gtcactagta 2280 ctggtgggaa ttgtgacact gtatttggga gtcatggtgc aggccgatag tggttgcgtc 2340 gtgagctgga aaaacaaaga actgaaatgt ggcagtggga ttttcatcac agacaacgtg 2400 cacacatgga cagaacaata caagttccaa ccagaatccc cttcaaaact agcttcagct 2460 atccagaaag ctcaagaaga gggaatttgt gggatccgct cggtaacaag attggagaac 2520 ctaatgtgga aacaaataac accagaattg aatcacattc tagcagaaaa tgaggtgaag 2580 ttgactatca tgacaggaga catcaaagga atcatgcagg caggaaaacg atctttgcgg 2640 cctcagccca ctgagctaaa gtattcatgg aaaacatggg gtaaagcaaa aatgctctct 2700 acagaatctt ataaccaaac ctttctcatt gatggccccg agacggcaga gtgccccaac 2760 acaaatagag cttggaactc gttggaggtt gaagactacg gctttggagt gttcaccacc 2820 aatatatggc taaaattgaa agaaaaacag gatgcattct gcgactcaaa actcatgtcg 2880 gcggccataa aagacaacag agccgtccat gccgacatgg gttactggat agaaagtgca 2940 ctcaatgaca catggaagat agagaaagcc tctttcattg aagtaaaaaa ctgccactgg 3000 ccaaaatcac acaccctctg gagcaatgga gtgctagaaa gtgagatgat aatcccaaag 3060 aatctcgctg gaccagtgtc tcaacacaac tatagaccag gctaccatac acaagtagca 3120 ggaccatggc acctaggtaa gcttgagatg gactttgatt tctgcgatgg aaccacagta 3180 atagtgactg aggactgcgg aaatagagga ccctctttga gaacaactac tgcctctgga 3240 aaactcataa cggaatggtg ctgccgatct tgcacattac cacccctaag atacagaggt 3300 gagaatgggt gctggtacgg gatggaaatc agaccattga aagagaaaga agaaaatctg 3360 gttaactcct tggtcacagc cggacatggg caggttgaca atttttcact gggagtcttg 3420 ggaatggcat tgttcctgga ggaaatgctt aggacccgag taggaacgaa acatgcaata 3480 ctactagttg cagtttcttt tgtgacattg atcacaggga acatgtcctt caaagaccta 3540 ggaagagtgg tggttatggt aggcgctacc atggcggatg acataggtat gggcgtgact 3600 tatcttgccc tactagcagc cttcaaagtt agaccaactt ttgcagctgg actactcttg 3660 agaaagctga cctccaagga attgatgatg actaccatag gaattgtact cctctcccaa 3720 agcactatac cagagaccgt tcttgaattg actgacgcgt tagccttagg catgatggtc 3780 cttaaaatag taagaaacat ggaaaagtat caattagcag tgactatcat ggctatcttg 3840 tgcgtcccaa acgcagtgat attgcaaaat gcatggaaag tgagttgcac aatactggca 3900 gtggtgtccg tttccccact gtttttgaca tcctcacagc aaaaaacgga ttggatacca 3960 ttagcattga caatcaaggg tctcaatcca acagccattt ttctaacaac cctctcaaga 4020 accaacaaga aaagaagctg gccactaaat gaggctatca tggcagtcgg aatggtgagc 4080 attttagcca gttccctcct aaaaaatgat rttcccatga caggaccact agtggctgga 4140 gggctcctca ctgtgtgcta cgtgctcact ggacgatcgg ccgacttgga actggagaga 4200 gcaaccgayg tcaaatggga agaccaggca gagatatcag gaagcagtcc aatcctgtca 4260 ataacaatat cagaagatgg tagcatgtcg ataaaaaatg aagaggaaga acaaacactg 4320 accatactca ttagaacagg attgctggtg atctcaggac tttttcctat atcaatacca 4380 gtcacggcag cagcatggta cctgtgggaa gtgaagaaac aacgggccgg agtattgtgg 4440 gacgttcctt cacctccacc catgggaaag gctgaactgg aagatggagc ctacagaatc 4500 aagcaaaaag ggattcttgg gtattctcag atcggagctg gagtttacaa agaaggaaca 4560 tttcatacaa tgtggcatgt cacacgtggc gctgttctaa tgcataaagg aaagaggatt 4620 gaaccttcat gggcggacgt caaaaaagat ctaatatcat atggaggagg ctggaagtta 4680 gaaggagaat ggaaggaagg agaagaagtc caggtgctgg cactggagcc tggaaaaaat 4740 ccaagagccg tccaaacaaa acctggtctt ttcaaaacca acaccggaac aataggtgcc 4800 gtatctctgg acttttcccc tggaacgtca ggatctccaa tcatcgacaa aaaaggaaaa 4860 gttgtgggtc tttatggcaa tggtgttgtc acaaggagtg gagcatatgt gagtgctata 4920 gcccaaactg aaaaaagcat tgaagacaac cccgagatca aagatgacat tttccgaaag 4980 agaagactga ccatcatgga cctccaccca ggagcgggaa agacgaaaag ataccttcca 5040 gccatagtca gagaagccat aaaacggggt ttgagaacat taatcttggc ccccactaga 5100 gttgtggcag ctgaaatgga ggaagctctt agaggacttc caataagata ccaaacccca 5160 gccatcagag ctgagcacac cgggcgggaa attgtggatc taatgtgtca tgccacattt 5220 accatgaggc tgctgtcacc agttagagtg ccaaactata acttgattat catggacgaa 5280 gcccatttca cagacccagc aagtatagca gctagaggat acatctcaac ccgagtggag 5340 atgggtgagg cagctgggat tttcatgaca gccactcccc cgggaagtag agatccattt 5400 cctcagagca atgcaccaat catagatgaa gaaagagaaa tccctgaacg ttcatggaat 5460 tctggacatg agtgggtcac ggattttaaa gggaagactg tttggttcgt tccaagcata 5520 aaagcaggaa atgatatagc agcttgcctg agaaaaaatg gaaagaaagt aatacaactt 5580 agtaggaaga cttttgattc tgagtatgtc aagactagaa ccaatgattg ggatttcgtg 5640 gttacaactg acatttcaga aatgggtgcc aatttcaaag ctgagagggt tatagacccc 5700 agacgctgca tgaaaccagt catactaaca gatggtgagg agcgggtgat tctggcagga 5760 cctatgccag tgacccactc tagcgcagca caaagaagag ggagaatagg aagaaatcca 5820 aaaaatgaaa atgaccagta catatacatg ggagaacctt tggaaaatga tgaagactgt 5880 gcacactgga aagaagccaa aatgctccta gataacatta acacaccaga aggaatcatt 5940 cctagtatgt ttgaaccaga gcgtgaaaaa gtggatgcca ttgatggcga ataccgtttg 6000 agaggagaag caaggaaaac ctttgtggac ttaatgagga gaggagactt accagtctgg 6060 ttggcttaca aagtggcagc tgaaggcatc aactatgcag acagaaggtg gtgttttgat 6120 ggaattaaga acaaccaaat cctggaagaa aatgtggaag ttgaaatctg gacaaaagaa 6180 ggggaaagga agaaattgaa acccagatgg ttggatgcta ggatctactc tgacccactg 6240 gcgctaaagg aattcaaaga atttgcagcc ggaagaaagt ctctgaccct gaacctaatc 6300 acagaaatgg gtaggcttcc aacattcatg actcagaaga cgagagacgc actggacaac 6360 ctagcagtgc tgcacacggc tgaagcaggt ggaagggcgt acaatcatgc tctcagtgaa 6420 ctgccggaga ccctagagac actgctttta ctgacacttc tggctacagt cacaggaggg 6480 atctttttat tcttgatgag cgggaggggc atagggaaga tgaccctggg aatgtgctgc 6540 ataatcacgg ctagcatcct tctatggtac gcacaaatac agccacactg gatagcagct 6600 tcaataatac tggagttttt tctcatagtt ttgcttattc cagaaccaga aaaacagaga 6660 acaccccaag acaaccaatt gacctacgtt gtcatagcca tcctcacagt ggtggccgca 6720 accatggcaa acgagatggg tttcctagaa aaaacgaaga aggatcttgg attaggaagc 6780 attgcaaccc agcaacccga gagcaacatc ctggacatag acctacgtcc cgcatcagct 6840 tggacgctgt atgccgtggc tacaacattc gttacaccaa tgctgagaca tagcattgag 6900 aattcctcag tgaatgtatc cctaacagct atagccaacc aagcaacagt gttaatgggt 6960 ctagggaaag gatggccatt gtcaaagatg gacatcggag ttccccttct cgccattgga 7020 tgttactcac aagtcaatcc cataactctt acagcagctc tcctcttatt ggtagcgcat 7080 tacgccatca ttgggccagg acttcaagca aaagcaacca gagaagccca gaaaagagca 7140 gcggcgggca tcatgaaaaa cccaaccgtc gatggaataa cagtgattga tctagatcca 7200 ataccttatg atccaaagtt tgaaaagcag ttgggacaag taatgctact aatcctctgc 7260 gtgactcaag tattgatgat gaggactaca tgggctctgt gtgaggcctt aaccttagct 7320 acagggccca tctccacact gtgggaagga aatccaggaa ggttctggaa cactaccatt 7380 gcggtgtcaa tggctaacat ctttagaggg agttacctgg ctggagctgg acttctcttt 7440 tccattatga agaacacaac caacacaaga aggggaactg gcaacatagg agagacgctt 7500 ggagagaaat ggaaaagccg attgaacgca ctgggaaaaa gtgaattcca gatctacaag 7560 aaaagtggaa tccaggaagt ggatagaacc ttagcaaaag aaggcatcaa aagaggagaa 7620 acggaccatc acgctgtgtc gcgaggctca gcaaaactga gatggttcgt tgagagaaac 7680 atggtcacac cagaagggaa ggtagtggac ctcggttgtg gcagaggagg ctggtcatac 7740 tattgtggag gactaaaaaa cgtaagagaa gtgaaaggcc taacaaaagg aggaccagga 7800 cacgaagaac ccatccctat gtcaacatat gggtggaatc tggtgcgtct tcagagtgga 7860 gttgacgtct tcttcattcc gccagaaaag tgtgacacat tgttgtgtga cataggggag 7920 tcatcaccaa atcccacagt ggaagcagga cgaacactca gagtccttaa cttagtagaa 7980 aactggttga acaacaacac tcaattttgc ataaaagttc tcaacccata tatgccctca 8040 gttatagaaa aaatggaagc actgcaaagg aaatatggag gagccttagt gagaaatcca 8100 ctctcacgga actccacaca tgagatgtac tgggtatcca atgcttctgg gaacatagtg 8160 tcatcagtga acatgatttc aaggatgttg atcaatagat tcacaatgag acacaagaaa 8220 gccacctacg agccggatgt tgacctcgga agcggaaccc gaaacattgg agttgaaagt 8280 gaaataccaa acctagacat aattgggaaa agaatagaaa aaataaagca agagcatgaa 8340 acatcatggc actacgacca agaccaccca tacaaaacgt gggcatacca tggtagctat 8400 gaaacaaaac agactggatc agcatcatcc atggtcaacg gagtggtcag gctgctgaca 8460 aaaccttggg acgttctccc cacggtgaca cagatggcaa tgacagacac gactccattt 8520 ggacaacagc gtgtttttaa agaaaaagtg gacacgagaa cccaagaacc gaaagaaggc 8580 acgaagaaac taatgagaat cacagcagag tggctgtgga aagaattagg gaagaaaaag 8640 acacctagga tgtgcaccag agaagaattc acaagaaagg tgagaagcaa tgcagccttg 8700 ggggccatat tcactgatga gaacaagtgg aagtcggcac gtgaggctgt tgaagatagt 8760 aggttttggg agctggttga caaggaaagg aatctccatc ttgaaggaaa gtgtgagaca 8820 tgtgtgtaca acatgatggg aaaaagagaa aagaagctag gggaattcgg caaggcaaaa 8880 ggcagcagag ccatatggta catgtggctt ggagctcgct tcctggagtt tgaagcccta 8940 ggattcctaa atgaagatca ctggttctct agagagaact ccctgagtgg agtggaagga 9000 gaagggctgc acaagctagg ttacattcta agagacgtga gcaagaaaga gggaggagcc 9060 atgtacgccg acgacacagc aggatgggac acaagaatca cactagaaga cctaaaaaat 9120 gaagaaatgg taacaaacca catggaagga gaacacaaga aattggccga ggctattttc 9180 aaactaacgt atcaaaacaa ggtggtgcgt gtgcaaagac caacaccaag aggcacagta 9240 atggacatca tatcgagaag agaccaaaga ggtagtgggc aagttggtac atatggactc 9300 aatactttca ccaatatgga agcccaatta atcagacaga tggagggaga aggagtcttt 9360 aaaaacatcc agcacctgac agtcacagaa gaagttgccg tgcaaaactg gttggcaaga 9420 gtggggcgcg aaaggttgtc aagaatggcc atcagtggag atgattgtgt tgtgaaacct 9480 ttagatgaca ggttcgcaaa cgctttgaca gctctaaatg acatgggaaa gattagaaaa 9540 gatatacaac aatgggaacc ttcaaaagga tggaacgatt ggacacaagt gcccttctgt 9600 tcacaccatt tccatgagct aatcatgaag gatggtcgtg tacttgttgt tccatgtaga 9660 aaccaagatg aattgattgg cagagcccga atctcccaag gagcagggtg gtctttgcgg 9720 gagacggcct gtttggggaa gtcttacgcc caaatgtgga gcttgatgta tttccacagg 9780 cgtgacctca ggctggcggc aaatgccatt tgctcggcag tgccatcaca ctgggttcca 9840 acaagtcgaa caacctggtc catacacgcc aaacatgaat ggatgacaac ggaagacatg 9900 ctgacagtct ggaacagggt gtggattcga gaaaacccat ggatggaaga caaaacccca 9960 gtggaatcat gggaggaaat cccatacttg ggaaaaagag aagaccaatg gtgcggctca 10020 ttgattgggt taacaagcag ggccacatgg gcaaagaaca tccaagcagc aataaatcag 10080 gttagatctc ttataggcaa tgaggaatac acagattaca tgccatccat gaaaagattt 10140 agaagagaag aggaagaagc aggagtcctg tgg 10173 <210> 23 <211> 10170 <212> DNA <213> Dengue virus type 3 <400> 23 atgaacaacc aacggaaaaa gacgggaaaa ccgtctatca atatgctgaa acgcgtgaga 60 aaccgtgtgt caactggatc acagttggcg aagagattct caagaggatt gctgaacggc 120 caaggaccaa tgaaattggt tatggcattt atagctttcc tcagatttct agccattcca 180 ccgacagcag gagtcttggc tagatggggt acctttaaga agtcgggggc tattaaggtc 240 ttaaaaggct tcaagaagga gatctcaaac atgctgagca ttatcaacaa acggaaaaag 300 acatcgctct gtctcatgat gatgttacca gcaacacttg ctttccactt aacttcacga 360 gatggagagc cgcgcatgat tgtggggaag aatgaaagag gaaaatccct acttttcaag 420 acagcctctg gaatcaacat gtgcacactc atagctatgg atctgggaga gatgtgtgat 480 gacacggtca cttacaaatg cccccacatt accgaagtgg agcctgaaga cattgactgc 540 tggtgcaacc ttacatcgac atgggtgact tatggaacat gcaatcaagc tggagagcat 600 agacgcgata agagatcagt ggcgttagct ccccatgttg gcatgggact ggacacacgc 660 actcaaacct ggatgtcggc tgaaggagct tggagacaag tcgagaaggt agagacatgg 720 gcccttaggc acccagggtt taccatacta gccctatttc ttgcccatta cataggcact 780 tccttgaccc agaaagtggt tatttttata ctattaatgc tggttacccc atccatgaca 840 atgagatgtg taggagtagg aaacagagat tttgtggaag gcctatcggg agctacgtgg 900 gttgacgtgg tgctcgagca cggtgggtgt gtgactacca tggctaagaa caagcccacg 960 ctggacatag agcttcagaa gaccgaggcc acccaactgg cgaccctaag gaagctatgc 1020 attgagggaa aaattaccaa cataacaacc gactcaagat gtcccaccca aggggaagcg 1080 attttacctg aggagcagga ccagaactac gtgtgtaagc atacatacgt ggacagaggc 1140 tggggaaacg gttgtggttt gtttggcaag ggaagcttgg tgacatgcgc gaaatttcaa 1200 tgtttagaat caatagaggg aaaagtggtg caacatgaga acctcaaata caccgtcatc 1260 atcacagtgc acacaggaga ccaacaccag gtgggaaatg aaacgcaggg agtcacggct 1320 gagataacac cccaggcatc aaccgctgaa gccattttac ctgaatatgg aaccctcggg 1380 ctagaatgct caccacggac aggtttggat ttcaatgaaa tgatctcatt gacaatgaag 1440 aacaaagcat ggatggtaca tagacaatgg ttctttgact tacccctacc atggacatca 1500 ggagctacag cagaaacacc aacttggaac aggaaagagc ttcttgtgac atttaaaaat 1560 gcacatgcaa aaaagcaaga agtagttgtt cttggatcac aagagggagc aatgcataca 1620 gcactgacag gagctacaga gatccaaacc tcaggaggca caagtatctt tgcggggcac 1680 ttaaaatgta gactcaagat ggacaaattg gaactcaaag ggatgagcta tgcaatgtgc 1740 ttgggtagct ttgtgttgaa gaaagaagtc tccgaaacgc agcatgggac aatactcatt 1800 aaggttgagt acaaagggaa agatgcaccc tgcaagattc ctttctccac ggaggatgga 1860 caaggaaaag ctcacaatgg cagactgatc acagccaatc cagtggtgac caagaaggag 1920 gagcctgtca acattgaggc tgaacctcct tttggagaaa gtaacatagt aattggaatt 1980 ggagacaaag ccctgaaaat caactggtac aggaagggaa gctcgattgg gaagatgttc 2040 gaggctactg ccagaggtgc aaggcgcatg gccatcttgg gagacacagc ctgggacttt 2100 ggatcagtgg gtggtgtttt gaattcatta gggaaaatgg tccaccaaat atttgggagt 2160 gcttacacag ccctatttgg tggagtctcc tggatgatga aaattggaat aggtgtcctc 2220 ttaacctgga tagggttgaa ctcaaaaaat acttctatgt cattttcatg catcgcgata 2280 ggaatcatta cactctatct gggagccgtg gtgcaagctg acatggggtg tgtcataaac 2340 tggaaaggca aagaactcaa atgtggaagt ggaattttcg tcactaatga ggtccacacc 2400 tggacagagc aatacaaatt tcaagcagac tcccccaaga gactggcaac agccattgca 2460 ggcgcttggg aaaatggagt gtgcggaatt aggtcaacaa ccagaatgga gaacctcttg 2520 tggaagcaaa tagccaatga actgaattac atattatggg aaaacaacat taaattaacg 2580 gtagttgtag gcgacataac tggggtctta gagcaaggga aaagaacact aacaccacaa 2640 cccatggagc taaaatattc ttggaaaaca tggggaaagg caaaaatagt gacagctgaa 2700 acacaaaatt cctctttcat aatagatggg ccaagcacac cggagtgtcc aagtgcctca 2760 agagcatgga atgtgtggga ggtggaggat tacgggttcg gagttttcac aaccaacata 2820 tggctgaaac tccgagaggt gtacacccaa ctatgtgacc ataggctaat gtcggcagcc 2880 gtcaaggatg agagggctgt acatgccgac atgggctatt ggatagaaag ccaaaagaat 2940 gggagttgga agctagaaaa agcatccttc atagaggtga aaacctgcac atggccaaaa 3000 tcacacactc tctggagcaa tggtgtgcta gagagtgaca tgattatccc aaagagtcta 3060 gctggtccca tttcgcaaca caaccacagg cccgggtacc acacccaaac ggcaggaccc 3120 tggcacttag gaaaattgga gctggacttc aactattgtg aaggaacaac agttgtcatc 3180 tcagaaaact gtgggacaag aggcccatca ttgagaacaa caacggtgtc agggaagttg 3240 atacacgaat ggtgctgccg ctcgtgcaca cttcctcccc tacgatacat gggagaagac 3300 ggctgctggt atggcatgga aatcagaccc attaatgaga aagaagagaa tatggtaaag 3360 tctctagcct cagcagggag tggaaaggtg gacaacttca caatgggtgt cttgtgtttg 3420 gcaatcctct ttgaagaggt gatgagagga aaatttggga aaaaacacat gattgcaggg 3480 gttctcttca cgtttgtgct cctcctctca gggcaaataa catggagaga catggcgcac 3540 acactcataa tgattgggtc caacgcctct gacagaatgg ggatgggcgt cacttaccta 3600 gctctaattg caacatttaa aattcagcca ctcctggctt tgggattctt cctgaggaaa 3660 ctgacatcta gagaaaattt attgctggga gttgggttgg ccatggcagc aacgttacga 3720 ctgccagagg acattgaaca gatggcgaat ggaattgctt tggggctcat ggctcttaaa 3780 ctgataacac aatttgaaac ataccaacta tggacggcat tagtttccct aacgtgttca 3840 aatacaattt tcacgttgac tgttgcctgg agaacagcca ctctgatttt agccggaatt 3900 tcgcttttgc cagtgtgcca gtcttcgagc atgaggaaaa cagattggct cccaatgact 3960 gtggcagcta tgggagctca acccctacca ctttttattt tcagtctgaa agatacactc 4020 aaaaggagaa gctggccact gaatgagggg gtgatggcag ttggacttgt gagcattcta 4080 gctagttctc tccttaggaa tgatgtgcct atggctggac cattagtggc tgggggcttg 4140 ctgatagcgt gctacgtcat aactggcacg tcagcagacc tcactgtaga aaaagcagca 4200 gatgtaacat gggaggaaga ggccgagcaa acaggagtgt cccacaattt aatggtcaca 4260 gttgatgatg atggaacaat gagaataaaa gatgacgaga ctgagaacat cttaacagtg 4320 cttttaaaaa cagcactact aatagtatca ggcatctttc catactccat acccgcaaca 4380 ctgttggtct ggcatacttg gcaaaagcaa acccaaagat ccggcgtcct atgggacgta 4440 cccagccccc cagagacaca gaaagcggaa ctggaagaag gggtctatag gatcaaacag 4500 caaggaattt ttgggaaaac ccaagtgggg gttggagtac agaaagaagg agttttccac 4560 accatgtggc atgtcacaag aggggcagtg ttgacacaca atgggaaaag actggaacca 4620 aactgggcta gcgtgaaaaa agatctgatt tcatacggag gaggatggag attgagtgca 4680 caatggaaaa agggggagga ggtgcaggtt attgccgtag agcctgggaa gaacccaaag 4740 aactttcaaa ccatgccagg catttttcag acaacaacag gggaaatagg agcaattgca 4800 ctggatttca agcctggaac ttcaggatct cccatcataa acagagaggg aaaggtagtg 4860 ggactgtatg gcaatggagt ggttacaaag aatggaggct atgtcagtgg aatagcgcaa 4920 acaaatgcag aaccagatgg accgacacca gagttggaag aagagatgtt caaaaagcga 4980 aatctaacca taatggatct ccatcctggg tcaggaaaga cgcggaaata tcttccagct 5040 attgttagag aggcaatcaa gagacgctta aggactctaa ttttggcacc aacaagggta 5100 gttgcagctg agatggaaga agcattgaaa gggctcccaa taaggtatca aacaactgca 5160 acaaaatctg aacacacagg aagagagatt gttgatctaa tgtgtcacgc aacgttcaca 5220 atgcgcttgc tgtcaccagt cagggttcca aactacaact tgataataat ggatgaggcc 5280 catttcacag acccagccag tatagcggct agagggtaca tatcaactcg tgtaggaatg 5340 ggagaggcag ccgcaatttt catgacagca acaccccctg gaacagctga tgcctttcct 5400 cagagcaacg ctccaattca agatgaagag agagacatac cggaacgctc atggaattca 5460 ggcaatgaat ggattactga ctttgttggg aagacagtgt ggtttgtccc tagcatcaaa 5520 gccggaaatg acatagcaaa ctgcttgcgg aaaaatggaa aaaaggttat tcaactcagc 5580 aggaagacct ttgacacaga atatcaaaag accaaactga atgattggga ctttgtggtg 5640 acaacagaca tttcagaaat gggagccaat ttcaaagcag atagagtgat cgacccaaga 5700 agatgtctca agccggtgat tttgacagat ggacccgagc gggtgatcct ggctggacca 5760 atgccagtca ccgtagcgag cgctgcgcaa aggagaggga gagttggcag gaacccacaa 5820 aaagaaaatg accagtacat attcatgggc cagcctctca acaatgatga agaccatgct 5880 cactggacag aagcaaaaat gctgctggac aacatcaaca caccagaagg gattatacca 5940 gctctctttg aaccagaaag ggagaagtca gccgccatag acggcgaata ccgcctgaag 6000 ggtgagtcca ggaagacttt cgtggaactc atgaggaggg gtgacctccc agtttggcta 6060 gcccataaag tagcatcaga agggatcaaa tatacagata gaaaatggtg ctttgatgga 6120 gaacgtaata atcaaatttt agaggagaat atggatgtgg aaatctggac aaaggaagga 6180 gaaaagaaaa aactgagacc taggtggctt gatgcccgca cttattcaga tcctttagca 6240 ctcaaagaat tcaaggattt tgcagctggc agaaagtcaa tcgcccttga tcttgtgaca 6300 gaaataggaa gagtgccttc acacttagcc cacagaacga gaaacgccct ggataatttg 6360 gtgatgctgc acacgtcaga acatggcggt agggcctaca ggcatgcagt ggaggaacta 6420 ccagaaacga tggaaacact cttactcctg ggactgatga tcttgttaac aggtggagca 6480 atgctcttct tgatatcagg taaagggatt ggaaagactt caataggact catttgtgta 6540 attgcttcca gcggcatgtt atggatggct gatgtcccac tccaatggat cgcatcggct 6600 atagtcctgg agttttttat gatggtgttg ctcataccag aaccagaaaa gcagagaact 6660 ccccaagaca accaactcgc atatgtcgtg ataggcatac ttacattggc tgcaatagta 6720 gcggccaatg aaatgggact gttggaaact acaaagagag atttaggaat gtctaaagaa 6780 ccaggtgttg tttctccaac cagctatttg gatgtggact tgcacccagc atcagcctgg 6840 acattgtacg ccgtggccac aacagtaata acaccaatgt tgagacacac catagagaat 6900 tccacagcaa atgtgtctct ggcagccata gctaaccagg cagtggtcct gatgggttta 6960 gacaaaggat ggccgatatc gaaaatggac ttgggcgtac cactattggc actgggttgc 7020 tattcacaag tgaacccact aactcttgca gcggcagtac ttttgctagt cacacattat 7080 gcaattatag gtccaggatt gcaggcaaaa gccacccgtg aagctcagaa aaggacagct 7140 gctggaataa tgaagaatcc aacggtggat ggaataatga caatagacct agatcctgta 7200 atatatgatt caaaatttga aaagcaacta ggacaggtca tgctcctggt tctgtgtgca 7260 gtccaacttt tattgatgag aacatcatgg gccttgtgtg aagttctaac cctagccaca 7320 ggaccaataa caacactctg ggaaggatca cctgggaagt tctggaacac cacgatagct 7380 gtttccatgg cgaacatctt tagagggagc tatttagcag gagctgggct wgctttttct 7440 atcatgaaat cagttggaac aggaaagaga ggaacagggt cacaaggtga aaccttagga 7500 gaaaagtgga aaaagaaatt aaatcagtta tcccggaaag agtttgacct ttacaagaaa 7560 tccggaatca ccgaagtgga tagaacagaa gccaaagaag ggttaaaaag aggagaaata 7620 acacaccatg ccgtgtccag aggcagcgca aaacttcaat ggttcgtgga gagaaacatg 7680 gtcattcctg aaggaagagt catagaccta ggctgtggaa gaggaggctg gtcatattac 7740 tgtgcaggac tgaaaaaagt tacagaagtg cgaggataca caaaaggcgg cccaggacac 7800 gaagaaccag tacctatgtc tacatacgga tggaacatag tcaagttaat gagtggaaag 7860 gatgtttttt atctgccacc tgaaaagtgt gataccctat tgtgtgacat tggagaatct 7920 tcaccaagcc caacagtgga agaaagcaga accataagag ttttgaagat ggttgaacca 7980 tggctaaaga acaaccagtt ttgcattaaa gtattgaacc catacatgcc aactgtgatt 8040 gagcacttag aaagactaca aaggaaacat ggaggaatgc ttgtgagaaa tccactctca 8100 cgaaactcca cgcacgaaat gtattggata tccaatggta caggcaatat cgtctcttca 8160 gtcaacatgg tatccagatt gctactgaac agattcacaa tgacacacag gagacccacc 8220 atagagaaag atgtggatct aggagcagga acccgacatg tcaatgcgga accagaaaca 8280 cccaacatgg atgtcattgg ggaaagaata aaaaggatca aagaggagca tagttcaaca 8340 tggcactatg atgatgaaaa tccttacaaa acgtgggctt accatggatc ctatgaagta 8400 aaagccacag gctcagcctc ctccatgata aatggagtcg tgaaactcct cacaaaacca 8460 tgggatgtgg tgcccatggt gacacagatg gcaatgacag atacaactcc attcggccag 8520 caaagagttt ttaaagagaa agtggacacc aggacaccta ggcccatgcc aggaacaaga 8580 aaggttatgg agatcacagc ggagtggctt tggaggaccc tgggaaggaa caaaagaccc 8640 agattatgca caagggagga attcacaaag aaggtcagaa ccaacgcagc tatgggcgct 8700 gtcttcacag aagagaacca atgggacagt gcgagagctg ctgttgagga cgaagaattt 8760 tggaaacttg tggacagaga acgtgaactc cacaaactgg gcaagtgtgg aagctgcgtt 8820 tacaacatga tgggcaagag agagaaaaaa cttggagagt ttggtaaagc aaaaggcagt 8880 agggctatat ggtacatgtg gttgggagcc aggtaccttg agttcgaggc gctcggattc 8940 ctcaatgaag accactggtt ctcgcgtgaa aactcttaca gtggagtaga aggagaagga 9000 ctgcacaagc tgggatacat cttgagagat atttccaaga tacccggagg agccatgtat 9060 gctgatgaca cagccggttg ggacacaaga ataacagaag atgacctgca caatgaggaa 9120 aaaatcacac agcagatgga ccctgaacac aggcagctag cgaacgctat attcaagctc 9180 acataccaaa acaaagtggt caaagtccaa cgaccaactc caaagggcac ggtaatggac 9240 atcatatcta ggaaagacca aagaggcagt ggacaggtgg gaacttatgg tctgaacaca 9300 ttcaccaaca tggaagccca gctaatcaga caaatggaag gagaaggcgt gttgtcaaag 9360 gcagacctcg agaaccccca tccgctagag aagaaaatta cacaatggtt ggaaactaaa 9420 ggagtggaaa ggttaaaaag aatggccatc agcggggatg attgcgttgt gaaaccaatc 9480 gacgacagat tcgccaatgc cctgcttgcc ctgaacgata tgggaaaggt tagaaaggac 9540 atacctcaat ggcagccatc aaagggatgg catgattggc aacaggtccc cttctgctcc 9600 caccactttc atgaattgat catgaaagat ggaagaaagt tggtagttcc ctgcagaccc 9660 caggacgaac taataggaag agcgagaatc tcccaaggag caggatggag ccttagagaa 9720 actgcatgtc tagggaaagc ctacgctcaa atgtgggctc tcatgtattt tcacagaaga 9780 gatcttagac tagcatccaa cgccatatgt tcagcagtac cagtccactg ggtccccacg 9840 agcagaacga catggtctat tcatgctcac catcagtgga tgactacaga agacatgctt 9900 actgtctgga acagggtgtg gatagaggac aatccatgga tggaagacaa aactccagtc 9960 acaacgtggg aagatgttcc atatctaggg aagagagaag accaatggtg cggatcactc 10020 ataggtctca cttccagagc aacctgggcc cagaacatac tcacagcaat ccaacaggtg 10080 agaagcctca taggcaatga agagtttctg gactacatgc cttcgatgaa gagattcagg 10140 aaggaggagg agtcagaggg agccatttgg 10170 <210> 24 <211> 10161 <212> DNA <213> Dengue virus type 4 <400> 24 atgaaccaac gaaaaaaggt ggttagacca cctttcaata tgctgaaacg cgagagaaac 60 cgcgtatcaa cccctcaagg gttggtgaag agattctcaa ccggactttt ttctgggaaa 120 ggacccttac ggatggtgct agcattcatc acgtttttgc gagtcctttc catcccacca 180 acagcaggga ttctgaaaag atggggacag ttgaagaaaa ataaggccat caggatactg 240 attggattca ggaaggagat aggccgcatg ctgaacatct tgaacgggag aaaaaggtca 300 acgataacat tgctgtgctt gattcccacc gtaatggcgt ttcacttgtc aacaagagat 360 ggcgaacccc tcatgatagt ggcaaaacat gaaaggggga gacctctctt gtttaagaca 420 acagagggga tcaacaaatg cactctcatt gccatggact tgggtgaaat gtgtgaggac 480 actgtcacgt ataaatgccc cttactggtc aataccgaac ctgaagacat tgattgctgg 540 tgcaatctca cgtctacctg ggtcatgtat gggacatgca cccagagcgg agaacggaga 600 cgagagaagc gctcagtagc tttaacacca cattcaggaa tgggattgga aacaagagct 660 gagacrtgga tgtcatcgga aggggcttgg aagcatgctc agagagtaga gagctggata 720 ctcagaaacc caggattcgc gctcttggca ggatttatgg cttatatgat tgggcaaaca 780 ggaatccagc gaactgtctt ctttgtccta atgatgctgg tcgccccatc ctacggaatg 840 cgatgcgtag gagtaggaaa cagagacttt gtggaaggag tctcaggtgg agcatgggtc 900 gatctggtgc tagaacatgg aggatgcgtc acaaccatgg cccagggaaa accaaccttg 960 gattttgaac tgactaagac aacagccaag gaagtggctc tgttaagaac ctattgcatt 1020 gaagcctcaa tatcaaacat aaccacggca acaagatgtc caacgcaagg agagccttat 1080 ctaaaagagg aacaagacca acagtacatt tgccggagag atgtggtaga cagagggtgg 1140 ggcaatggct gtggcttgtt tggaaaagga ggagttgtga catgtgcgaa gttttcatgt 1200 tcggggaaga taacaggcaa tttggtccaa attgagaacc ttgaatacac agtggttgta 1260 acagtccaca atggagacac ccatgcagta ggaaatgaca catccaatca tggagttaca 1320 gccacgataa ctcccaggtc accatcggtg gaagtcaaat tgccggacta tggagaacta 1380 acactcgatt gtgaacccag gtctggaatt gactttaatg agatgattct gatgaaaatg 1440 aaaaagaaaa catggcttgt gcataagcaa tggtttttgg atctacctct accatggaca 1500 gcaggagcag acacatcaga ggttcactgg aattacaaag agagaatggt gacatttaag 1560 gttcctcatg ccaagagaca ggatgtgaca gtgctrggat ctcaggaagg agccatgcat 1620 tctgccctcg ctggagccac agaagtggac tccggtgatg gaaatcacat gtttgcagga 1680 catctcaagt gcaaagtccg tatggagaaa ttgagaatca agggaatgtc atacacgatg 1740 tgttcaggaa agttctcaat tgacaaagag atggcagaaa cacagcatgg gacaacagtg 1800 gtgaaagtca agtatgaagg tgctggagct ccgtgtaaag tccccataga gataagagat 1860 gtgaacaaga aaaaagtggt tgggcgtatc atctcatcca cccctttggc tgagaatacc 1920 aacagtgcaa ccaacataga gttagaaccc ccctttgggg acagctacat agtgataggt 1980 gttggaaaca gtgcattaac actccattgg ttcaggaaag ggagttccat tggcaagatg 2040 tttgagtcca catacagagg tgcaaaacga atggccattc taggtgaaac agcttgggat 2100 tttggttccg ttggtggact gttcacatca ttgggaaagg ctgtgcacca ggtttttgga 2160 agtgtgtata caaccatgtt tggaggagtc tcatggatga ttagaatcct aattgggttc 2220 ctartgttgt ggattggcac gaactcaagg aacacttcaa tggctatgac gtgcatagct 2280 gttggaggaa tcactctgtt tctgggcttc acagttcaag cagacatggg ttgtgtggtg 2340 tcatggagtg ggaaagaatt gaagtgtgga agcggaattt ttgtggttga caacgtgcac 2400 acttggacag aacagtacaa atttcaaccg gagtccccag cgagactagc gtctgcaata 2460 ttgaatgccc acaaagatgg ggtctgtgga attagatcaa ccacgaggct ggaaaatgtc 2520 atgtggaagc aaataaccaa cgagctaaat tatgttctct gggaaggagg acatgacctc 2580 actgtagtgg ctggggatgt gaagggggtg ttgaccaaag gcaagagagc actcacaccc 2640 ccagtgaatg atctgaaata ttcatggaag acatggggaa aagcaaaaat cttcacccca 2700 gaagcaagaa atagcacatt tttaatagac ggaccagaca cctccgaatg ccccaatgaa 2760 cgaagagcat ggaactttct tgaggtggaa gactatggat ttggcatgtt cacgaccaac 2820 atatggatga aattccgaga aggaagttca gaagtgtgtg accacaggtt aatgtcagcg 2880 gcaattaaag atcagaaagc tgtgcatgct gacatgggtt attggataga gagctcaaaa 2940 aaccagacct ggcagataga gaaagcatct cttattgaag tgaaaacatg tctgtggccc 3000 aagacccaca cattgtggag caatggagtg ctggaaagcc agatgctcat tccaaaatca 3060 tatgcgggcc ctttttcaca scacaattac cgccagggct atgccacgca aaccgtgggc 3120 ccatggcact taggcaaatt agagatagac tttggagaat gccccggaac aacagtcgca 3180 attcaggagg attgtgacca tagaggccca tctttgagga ccaccactgc atctggaaaa 3240 ctagtcacgc aatggtgctg ccgctcctgc acgatgcctc ccttaaggtt cttgggagaa 3300 gatgggtgct ggtatgggat ggagattagg cccttgagtg aaaaagaaga gaacatggtc 3360 aaatcacagg taacggccgg acagggcaca tcagaaactt tttctatggg tctgttgtgc 3420 ctgaccttgt ttgtggaaga atgcttgagg agaagagtca ctaggaaaca catgatattg 3480 gttgtggtga tcactctttg tgccatcatc ctaggaggcc tcacatggat ggacttayta 3540 cgagccctca tcatgttggg ggacactatg tctggtagaa taggaggaca gatccaccta 3600 gccatcatgg cagtgttcaa gatgtcacca ggataygtgc tgggtgtgtt tttaaggaaa 3660 ctcacttcaa gagagacagc actaatggta ataggaatgg ccatgacaac ggtgctttca 3720 attccacatg accttatgga actcattgat ggaatatcac tggggctaat tttgctaaaa 3780 atagtgacac attttgacaa cacccaagtg ggaaccttag ccctttcctt gaccttcata 3840 agatcaacaa tgccattggt catggcttgg aggaccatta tggctgtgtt gtttgtggtc 3900 acactcattc ctttgtgcag gacaagctgt cttcaaaaac agtctcattg ggtagaaata 3960 acagcactca tcctaggagc ccaagctctg ccagtgtacc taatgactct tatgaaagga 4020 gcctcaagaa gatcttggcc tcttaacgag ggcataatgg ctgtgggttt ggttagtctc 4080 ttaggaagcg ctcttttaaa gaatgatgtc cctttagctg gcccaatggt ggcaggaggc 4140 ttacttctgg cggcttacgt gatgagtggt agctcagcag atctgtcact agagaaggcc 4200 gccaatgtgc agtgggatga aatggcagac ataacaggct caagcccaat catagaagtg 4260 aagcaggatg aagatggctc tttctccata cgggacgtcg aggaaaccaa tatgataacc 4320 cttttggtga aactggcact gataacagtg tcaggtctct accccttggc aattccagtc 4380 acaatgacct tatggtacat gtggcaagtg aaaacacaaa gatcaggagc cctgtgggac 4440 gtcccctcac ccgctgccac tcaaaaagcc gcactgtctg aaggagtgta caggatcatg 4500 caaagagggt tatttgggaa aactcaggtt ggagtaggga tacacatgga aggtgtattt 4560 cacacaatgt ggcatgtaac aagaggatca gtgatctgcc atgagactgg gagattggag 4620 ccatcttggg ctgacgtcag gaatgacatg atatcatacg gtgggggatg gagacttgga 4680 gacaaatggg acaaagaaga agatgttcag gtcctcgcca tagaaccagg aaaaaatcct 4740 aaacatgtcc aaacgaaacc cggccttttc aagaccctaa ctggagaaat tggagcagta 4800 acattagatt tcaaacccgg aacgtctggt tctcccatca tcaacaggaa aggaaaagtc 4860 atcggactct atggaaatgg aktagttacc aaatcaggtg attacgtcag tgccataacg 4920 caagccgaaa gaattggaga gccagattat gaagtggatg aggacatttt tcgaaagaaa 4980 agattaacta taatggactt acaccccgga gctggaaaga caaaaagaat tcttccatca 5040 atagtgagag aagccttaaa aaggaggctg cgaaccttga ttttggctcc cacgagagtg 5100 gtggcggccg agatggaaga ggccctacgt ggactgccaa tccgttatca gaccccagct 5160 gtgaaatcag aacacacagg aagagagatt gtagacctca tgtgtcatgc aaccttcaca 5220 acaagacttt tgtcatcaac cagagttcca aattacaacc tcatagtgat ggatgaagca 5280 catttcaccg atccttctag tgtcgcggct agaggataca tctcgaccag ggtggaaatg 5340 ggagaggcag cagccatctt catgaccgca acccctcccg gagcgacaga tccctttccc 5400 cagagcaaca gcccaataga agacatcgag agggaaattc cggaaaggtc atggaacaca 5460 gggttcgact ggataacaga ctaccaaggg aaaactgtgt ggtttgttcc cagcataaaa 5520 gctggaaatg acattgcaaa ttgtttgaga aagtcgggaa agaaagttat ccagttgagt 5580 aggaaaacct ttgatacaga gtatccaaaa acgaaactca cggactggga ttttgtggtc 5640 actacagaca tatctgaaat gggggccaat tttagagctg ggagagtgat agaccctagg 5700 agatgcctca agccagttat cctaacagat gggccagaga gagtcatttt agcaggtcct 5760 attccagtga ctccagcaag cgctgctcag agaagagggc gaataggaag gaacccagca 5820 caagaagacg accaatacgt tttctccgga gacccactaa aaaatgatga agatcatgcc 5880 cactggacag aagcaaagat gctgcttgac aatatctaca ccccagaagg gatcattcca 5940 acattgtttg gtccggaaag ggaaaaaacc caagccattg atggagagtt tcgcctcaga 6000 ggggaacaaa ggaagacttt tgtggaatta atgaggagag gagaccttcc ggtgtggctg 6060 agctataagg tagcttctgc tggcatttct tacaaagatc gggaatggtg cttcacaggg 6120 gaaaggaata accaaatttt agaagaaaac atggaggttg aaatttggac tagagaggga 6180 gaaaagaaaa agctaaggcc aagatggtta gatgcacgtg tatacgctga ccccatggct 6240 ttgaaggatt ttaaggagtt tgctagtgga aggaagagca taactctcga catcctaaca 6300 gagattgcca gtttgccaac ttacctttcc tctagggcca agctcgccct tgataacata 6360 gtcatgctcc acacaacaga aagaggaggg agggcctacc aacacgccct gaacgaactc 6420 ccggagtcac tggaaacact tatgcttgta gctttactag gtgctatgac agcaggtatc 6480 ttcctgtttt tcatgcaagg gaaaggaata gggaaattgt caatgggttt gataaccatt 6540 gcggtggcta gtggcttgtt ctgggtagca gaaattcaac cccagtggat agcggcctca 6600 atcatactag agttttttct catggtactg ttgataccgg aaccagaaaa acaaaggacc 6660 ccacaagaca atcaattgat ctacgtcata ttgaccattc tcaccattat tggtctcata 6720 gcagccaacg agatggggct gattgaaaaa acaaaaacgg attttgggtt ttaccaggta 6780 aaaacagaaa ccaccatcct cgatgtggac ttgagaccag cttcagcatg gacgctctat 6840 gcagtagcca ccacawttct gactcccatg ctgagacaca ccatagaaaa cacgtcggcc 6900 aacctatctc tagcagccat tgccaaccag gcggccgtcc taatggggct tggaaaagga 6960 tggccgctcc acagaatgga cctcggtgtg ccgctgttag caatgggatg ctattctcaa 7020 gtgaacccaa caactttgac agcatcctta gtcatgcttt cagtccatta tgcaataata 7080 ggtccaggat tgcaggcaaa agccacaaga gaggcccaga aaaggacagc tgctgggatc 7140 atgaaaaacc ccacggtgga cgggataaca gtaatagatc tagaaccaat atcctatgac 7200 ccaaaatttg aaaagcaatt agggcaggtc atgctactcg tcttgtgtgc tggacaacta 7260 ctcttgatga gaacaacatg ggctttctgt gaagtcttga ctttggccac aggaccaatc 7320 ttgaccttgt gggagggcaa cccgggaagg ttttggaaca cgaccatagc cgtatccacc 7380 gccaacattt tcaggggaag ttacctggcg ggagctggac tggctttttc actcataaag 7440 aatgyacaaa cccctaggag gggaactggg accacaggag agacactggg agagaagtgg 7500 aagagacagc taaactcatt akacagaaaa gagtttgaag agtataaaag aagtggaata 7560 ctagaagtgg acaggactga agccaagtct gccctgaaag atgggtctaa aatcaagcat 7620 gcagtatcta gagggtccag taagattaga tggattgttg agagagggat ggtaaagcca 7680 aaagggaaag ttgtagatct tggctgtggg agaggaggat ggtcttatta catggcgacg 7740 ctcaagaacg tgactgaagt gaaagggtat acaaaaggag gtccaggaca tgaagaaccg 7800 attcccatgg ctacttatgg ctggaatttg gtcaaactcc attcaggggt tgacgtgttc 7860 tacaaaccca cagagcaagt ggacaccctg ctctgtgata ttggggagtc atcttctaat 7920 ccaacaatag aggaaggaag aacattaaga gttttgaaga tggtggagcc atggctctct 7980 tcaaaacctg aattctgcat caaagtcctt aacccctaca tgccaacagt catagaagag 8040 ctggagaaac tgcagagaaa acatggtggg aaccttgtca gatgcccgct gtccaggaac 8100 tccacccatg agatgtattg ggtgtcagga gcgtcgggaa acattgtgag ctctgtgaac 8160 acaacatcaa agatgttgtt gaacaggttc acaacaaggc ataggaaacc cacttatgag 8220 aaggacgtag atcttggggc aggaacgaga agtgtctcca ctgaaacaga aaaaccagac 8280 atgacaatta ttgggagaag gcttcagcga ttgcaagagg agcacaaaga aacctggcat 8340 tatgatcagg aaaacccata cagaacctgg gcgtatcatg gaagctatga agctccttcg 8400 acaggctctg catcctccat ggtgaacggg gtagtaaaac tgctaacaaa accttgggat 8460 gtggttccaa tggtgaccca gttagccatg acagacacaa ccccttttgg gcaacaaaga 8520 gtgttcaaag agaaggtgga taccagaaca ccacaaccaa aacccggtac acgaatggtt 8580 atgaccacga cagccaattg gctgtgggcc ctccttggga agaagaaaaa tcccagactg 8640 tgcacaaggg aagagttcat ctcaaaagtt agatcaaacg cagccatagg cgcagtcttt 8700 caggaagaac agggatggac atcagccagt gaagctgtga atgacagccg gttttgggaa 8760 ctggttgaca aagaaagggc cctacaccag gaagggaaat gtgaatcgtg tgtctacaac 8820 atgatgggaa aacgtgagaa aaagttagga gagtttggca gagccaaggg aagccgagca 8880 atctggtaca tgtggctggg agcgcggttt ctggaatttg aagccctggg ttttttgaat 8940 gaagatcact ggtttggcag agaaaattca tggagtggag tggaagggga aggtctgcac 9000 agattgggat atatcctgga ggagatagac aagaaggatg gagacctaat gtatgctgat 9060 gacacagcag gctgggacac aagaatcact gaggatgacc ttcaaaatga agaactgatc 9120 acggaacaga tggcccccca ccacaagatc ctagccaaag ccattttcaa actaacctat 9180 caaaacaaag tggtgaaagt cctcagaccc acaccgagag gagcggtgat ggatatcata 9240 tccaggaaag accaaagagg tagtggacaa gttggaacat atggtttgaa cacattcacc 9300 aacatggaag ttcaactcat ccgccaaatg gaagctgaag gagtcatcac acaagatgac 9360 atgcagaacc caaaagggtt gaaagaaaga gttgagaaat ggctgaaaga gtgtggtgtc 9420 gacaggttaa agaggatggc aatcagtgga gacgattgcg tggtgaagcc cctggatgag 9480 aggtttggca cttccctcct cttcttgaac gacatgggaa aggtgaggaa agacattccg 9540 cagtgggaac catctaaggg atggaaaaac tggcaagagg ttcctttttg ctcccaccac 9600 tttcacaaga tcttcatgaa ggatggccgc tcactagttg ttccatgtag aaaccaggat 9660 gaactgatag ggagagccag aatctcgcag ggggctggat ggagcttaag agaaacagcc 9720 tgcctgggca aagcttacgc ccagatgtgg tcgctcatgt acttccacag aagggatctg 9780 cgtttagcct ccatggccat atgctcagca gttccaacgg aatggtttcc aacaagcaga 9840 acaacatggt caatccacgc tcatcatcag tggatgacca ctgaagatat gctcaaagtg 9900 tggaacagag tgtggataga agacaaccct aatatgactg acaagactcc agtccattcg 9960 tgggaagata taccttacct agggaaaaga gaggatttgt ggtgtggatc cctgattgga 10020 ctttcttcca gagccacctg ggcgaagaac attcacacgg ccataaccca ggtcagaaac 10080 ctgatcggaa aagaggaata cgtggattac atgccagtaa tgaaaagata cagcgctcct 10140 tcagagagtg aaggagttct g 10161 <210> 25 <211> 10176 <212> DNA <213> Dengue virus type 1 <400> 25 atgaacaacc aacgaaaaaa gacggctcga ccgtctttca atatgctgaa acgcgcgaga 60 aaccgcgtgt caactgtttc acagttggcg aagagattct caaaaggatt gctctcaggc 120 caaggaccca tgaaattggt gatggctttc atagcattcc taagatttct agccataccc 180 ccaacagcag gaattttggc tagatggggc tcattcaaga agaatggagc gatcaaagtg 240 ctacggggtt tcaagaaaga aatctcaaac atgttgaata taatgaatag aaggaaaaga 300 tctgtgacca tgctccttat gctgatgcct acagccttgg cgttccattt gaccacacga 360 gggggagagc cgcacatgat agtcagcaag caggaaagag gaaagtcact tttgtttaag 420 acctcagcag gtgtcaacat gtgcaccctt atagcgatgg atttgggaga gttatgtgag 480 gacacaatga cttacaaatg ccctcgaatc actgaggcgg aaccagatga cgttgattgt 540 tggtgcaatg ccacagacac atgggtgacc tatggaacat gttcccaaac tggcgagcac 600 cgacgagaca aacgttccgt cgcactggcc ccacacgtgg gacttggttt ggaaacaaga 660 actgaaacgt ggatgtcctc tgaaggcgct tggaaacaga tacaaagagt ggagacttgg 720 gccctgagac acccaggatt cacggtgata gccctttttc tagcacatgc cataggaaca 780 tccatcaccc agaaagggat tattttcatt ttgttaatgc tggtaacacc atccatggcc 840 atgcgatgcg tgggaatagg cagcagggac ttcgtggaag gactgtcagg agcaacttgg 900 gtagatgtgg tactggaaca tggaagttgc gtcaccacca tggcaaaaga caaaccaaca 960 ttggacattg aactcttgaa gacggaagtc acaaaccctg ccgtcctgcg caaactgtgc 1020 attgaagcta aaatatcaaa caccaccacc gactcaagat gtccaacaca aggagaagcc 1080 acactggtgg aagaacaaga cgcgaacttt gtgtgtcgac gaacgtttgt ggacagaggc 1140 tggggcaatg gctgtgggct cttcggaaaa ggaagcctac taacgtgtgc taagttcaag 1200 tgtgtgacaa aactggaagg aaagatagtt caatatgaaa acttgaaata ttcagtaata 1260 gtyaccgtcc acactggaga ccagcatcag gtgggaaatg aaagcacaga acatgggaca 1320 actgcaacta taacacctca agctcctacg acggaaatac agctgaccga ctacggagct 1380 cttacattgg attgttcacc tagaacagga ctagacttta atgaaatggt gttgttgaca 1440 atgraaaaaa ratcatggct agtccacaaa caatggtttc tagacttacc actgccttgg 1500 acctcgggag cctcaacatc acaagagact tggaacagac aagatttgct ggtgacattt 1560 aagacagctc atgcaaagaa gcaggaagta gtcgtactag gatcacaaga aggagcaatg 1620 cacactgcgt tgaccggagc gacagaaatc caaacgtctg gaacgacaac aatttttgca 1680 ggacacttga aatgtagact aaggatggac aaactgactc taaaagggat gtcatatgtg 1740 atgtgcacag gctcattcaa gctagagaaa gaagtggctg agacccagca tggaaccgtt 1800 ctagtgcaga ttaaatacga aggaacagat gcaccatgca agatcccttt ttcgacccaa 1860 gatgaaaaag gagtaaccca gaatgggaga ttgataacag ccaaccccat agtcactgac 1920 aaagaaaaac cagtcaacat tgaggcagaa ccgccttttg gtgagagtta catcgtgata 1980 ggagcaggtg aaaaagcttt gaaactaagc tggttcaaga aaggaagcag catagggaaa 2040 atgtttgagg caactgccag aggagcacga aggatggcca tactgggaga caccgcatgg 2100 gactttggtt ctataggagg agtgttcacg tctgttggaa aattagtaca ccagattttt 2160 ggaactgcat atggagtttt gttcagcggt gtttcctgga ccatgaaaat aggaataggg 2220 gttctgctga catggctagg attaaactca aggaacacgt ccctctcgat gacgtgcatt 2280 gcagttggcc tagtaacact atacctagga gtcatggttc aggcggattc aggatgcgta 2340 attaattgga aaggtagaga actcaaatgt ggaagtggca tttttgtcac caatgaagtt 2400 cacacttgga cagagcaata caaatttcaa gctgactccc caaagagact atcagcagcc 2460 atcgggaagg catgggagga aggtgtgtgt ggaattcgat cagcaactcg tctcgagaac 2520 atcatgtgga agcaaatatc aaatgaactg aaccacatct tacttgaaaa tgacatgaaa 2580 ttcacagtgg ttgtaggaga tgttgctggg atcttggctc aaggaaagaa aatgattagg 2640 ccacaaccca tggaacacaa atactcgtgg aaaagctggg gaaaggctaa aatcataggg 2700 gcagatgtac agaactccac cttcatcatt gacggcccaa acaccccaga atgccctgat 2760 gaccaaagag catggaacat ttgggaagtt gaggactatg gatttggaat tttcacgaca 2820 aacatatggc tgaaattgcg tgactcctac acccaattgt gtgaccaccg gctaatgtca 2880 gctgccatca aggacagcaa ggcagttcac gctgacatgg ggtactggat agaaagtgaa 2940 aagaacgaga cctggaagct ggcaagagcc tcgttcatag aagttaaaac atgtatttgg 3000 ccaaaatccc acactctatg gagcaacgga gttctggaaa gtgaaatgat aattccaaag 3060 atctatggag gaccaatatc tcagcataac tacagaccag gatatttcac acaaacggta 3120 gggccgtggc acctaggcaa gttggaactg gattttgact tgtgtgaggg taccacagtc 3180 gttgtggatg aacattgtgg aaatcgagga ccatctctta ggaccacaac agtcacagga 3240 aagataattc atgaatggtg ttgtagatct tgtacgctac cacccttacg tttcagagga 3300 gaagatgggt gctggtacgg tatggaaatc agaccagtca aggaaaagga agagaatcta 3360 gtcaaatcaa tggtctctgc agggtcaggg gaagtggaca gcttttcact aggactgcta 3420 tgcatatcaa taatgatcga agaggtaatg agatccagat ggagtagaaa aatgctgatg 3480 actggaacac tggctgtgtt cctccttctc ataatgggac aattgacatg gaatgatctg 3540 atcagattat gcatcatggt tggagccaac gcttccgata gaatggggat gggaacgacg 3600 tacctagctc tgatggccac ttttaaaatg agaccaatgt ttgctgtagg gctattattc 3660 cgcagactaa catccagaga agttcttctt ctaacaattg gattgagtct agtggcatct 3720 gtggagttac caaattcctt ggaggagctg ggggatggac ttgcaatggg cattatgatt 3780 ttaaaattat tgactgactt tcaatcacat cagttgtggg ctaccttgct gtccttgaca 3840 tttatcaaaa caacgttttc cttgcactat gcatggaaga caatggctat ggtactgtca 3900 attgtatctc tcttcccctt atgcctgtcc acgacctccc agaaaacaac atggcttccg 3960 gtgctattgg gatctcttgg atgcaaacca ctaaccatgt ttcttatagc agaaaacaaa 4020 atctggggaa ggaaaagttg gcccctcaat gaagggatca tggctgttgg aatagtcagc 4080 atcctactaa gttcactcct caagaatgat gtgccgctag ctgggccact aatagctgga 4140 ggcatgctaa tagcatgtta tgttatatct ggaagctcag ccgacctatc actagagaaa 4200 gcagctgagg tctcctggga agaagaagca gaacactctg gtgcctcaca caacatatta 4260 gtggaggtcc aagatgatgg aaccatgaag ataaaggatg aagagagaga tgacacgcta 4320 accattctcc ttaaagcaac tctgctagca gtttcagggg tgtacccatt atcaatacca 4380 gcgacccttt ttgtgtggta cttttggcag aaaaagaaac agagatctgg agtgttatgg 4440 gacacaccta gccctccaga agtggaaaga gcagttcttg atgatggtat ctatagaatt 4500 atgcagagag gactgttggg caggtcccaa gtaggggtag gagtttttca agaaaacgtg 4560 ttccacacaa tgtggcatgt caccagggga gctgtactca tgtatcaagg gaagagactg 4620 gaaccgagct gggccagtgt caaaaaagac ctgatctcat atggaggagg ttggaggctt 4680 caaggatcct ggaacacggg agaagaagtg caggtgattg ctgttgaacc agggaaaaac 4740 cccaaaaatg tacaaacagc gccgggcacc tttaagaccc ctgaaggtga agttggagcc 4800 attgccctag attttaaacc cggcacatct ggatccccca tcgtgaacag agaaggaaaa 4860 atagtaggtc tttatggaaa tggagtagtg acaacaagtg gaacctacgt cagtgccata 4920 gctcaagcca aagcatcaca agaagggccc ctaccagaga ttgaagacga agtgtttagg 4980 aaaagaaaat taacaattat ggacctacat ccaggatcgg ggaaaacaag aagatatctt 5040 ccagccatag tccgtgaggc cataaaaagg aagctgcgca cactaattct ggctcccaca 5100 agggttgtcg cttccgaaat ggcagaagcg ctcaagggaa tgccaataag gtaccaaaca 5160 acagcagtga agagtgaaca cacaggaaaa gagatagttg acctcatgtg tcacgccact 5220 ttcaccatgc gcctcctgtc tcccgtgaga gttcccaatt acaacatgat tatcatggat 5280 gaagcacatt tcactgatcc atccagcata gcggccagag ggtacatctc aacccgagtg 5340 ggcatgggtg aagcagctgc gattttcatg acagccactc ccccaggatc agtggaggcc 5400 tttccacaga gcaacgcagt tatccaagat gaggaaagag acattcctga gagatcatgg 5460 aactcaggct atgagtggat cactgacttc ccaggtaaaa cagtctggtt tgttccaagc 5520 attaaagcag gaaatgacat tgccaactgc ttaagaaaga atggaaaacg ggtgattcaa 5580 ttgagcagga aaacctttga tacagagtac caaaaaacaa aaaataacga ctgggattat 5640 gtcgtcacaa cagacatctc cgaaatggga gcaaatttcc gggccgacag ggtgatagac 5700 ccaagacggt gtctgaaacc ggtaatacta aaagatggtc cagagcgtgt cattctagca 5760 ggaccgatgc cagtgactgt ggccagtgcc gcccagagga gaggaagaat tggaaggaac 5820 cacaataagg aaggtgacca gtacatctac atgggacagc ctttaaacaa cgatgaagat 5880 cacgctcact ggacagaagc aaaaatgctc cttgataata taaacacacc agaagggatt 5940 atcccagccc tcttcgagcc ggagagagaa aagagtgcag caatagacgg ggaatacaga 6000 ctgcggggtg aagcaaggaa aacgtttgtg gagctcatga gaagaggaga tctacctgtc 6060 tggctatcct acaaagttgc ctcagaaggc ttccagtact ctgacagaag atggtgcttt 6120 gacggggaaa ggaacaacca ggtgttggag gagaacatgg acgtggagat ttggacaaaa 6180 gaaggagaaa gaaagaaact acgaccccgc tggctggatg ccagaacata ctcagaccca 6240 ctggccctgc gcgagtttaa agagtttgca gcaggaagaa gaagcgtctc aggtgatcta 6300 atactagaaa tagggaaact tccacaacac ttgacgcaaa gggcccagaa tgccttggac 6360 aacctggtca tgttgcacaa ctccgaacaa ggaggaagag cctatagaca tgcaatggaa 6420 gaactgccag acaccataga aacgttgatg ctcctagctt tgatagctgt gttaactggt 6480 ggagtgacgc tgttcttcct atcaggaaag ggcctaggga aaacatctat cggcctactc 6540 tgcgtgatgg cttcaagcgt actgctatgg atggccagtg tggagcccca ttggatagcg 6600 gcctccatca tactggagtt cttcctgatg gtgttgctta ttccagagcc agacagacaa 6660 cgcactccgc aggacaacca gctggcatat gtggtgatag gtttgttatt catgatactg 6720 acagtagcag ccaatgagat gggactgctg gaaaccacaa agaaagactt agggattggc 6780 catgtggctg ttgaaaatca ccaccatgcc gcaatgctgg acgtagactt acatccagct 6840 tcagcctgga ccctctatgc agtggccaca acaattatca ctcccatgat gaggcacaca 6900 atcgaaaaca caacggcaaa catttccctg acagccattg caaaccaggc agctatattg 6960 atgggacttg acaaaggatg gccaatatcg aagatggaca taggagttcc acttctcgcc 7020 ttggggtgct attcccaggt gaatccactg acgctgacag cagcggtgtt katgctagtg 7080 gctcattacr ccataattgg acctggactg caagcaaaag ctactagaga agctcaaaaa 7140 aggacagcgg ccggaataat gaaaaatcca accattgatg gaattgttgc aatagatttg 7200 gaccctgtgg tttatgatgc aaaatttgag aaacaactag gccaaataat gttgttgata 7260 ctatgcacat cacagatcct cttgatgcgg actacatggg ccttgtgcga atccatcaca 7320 ctggccactg gacctctgac cacgctttgg gagggatctc caggaaaatt ttggaacacc 7380 acgatagcgg tttccatggc aaacattttc agaggaagtt atctagcagg agcaggtctg 7440 gccttctcat taatgaaatc tctaggagga ggtaggagag gcacaggagc ccaaggggaa 7500 acactgggag agaaatggaa aagacagctg aaccaactga gcaagtcaga attcaacacc 7560 tataaaagga gtgggattat ggaagtggac agatcygaag ccaaagaggg actgaaaaga 7620 ggagaaacaa ccaaacatgc agtgtcgaga ggaaccgcta aactgaggtg gtttgtggag 7680 aggaaccttg tgaaaccaga agggaaagtc atagacctcg gttgtggaag aggtggctgg 7740 tcatactatt gcgctgggct gaagaaagtc acagaagtga agggatatac aaaaggagga 7800 cctggacatg aggaaccaat cccaatggcg acctatggat ggaacctagt aaaactgcac 7860 tccgggaaag acgtattctt tataccacct gagaaatgtg ataccctttt gtgtgatatt 7920 ggtgagtcct ctccaaaccc aactatagag gaaggaagaa cgctacgcgt tctaaagatg 7980 gtggaaccat ggctcagagg aaaccaattt tgcataaaaa ttctgaatcc ctacatgcca 8040 agtgtggtgg aaactctgga gcaaatgcaa agaaaacatg gaggaatgct agtgcgaaat 8100 ccactttcaa gaaattctac tcatgaaatg tattgggttt catgtggaac aggaaacatt 8160 gtgtcagcag taaacatgac atccagaatg ttgctaaatc gattcacaat ggcccacagg 8220 aaaccaacat atgaaagaga cgtggaccta ggcgccggaa caagacatgt ggcagtggaa 8280 ccagaggtag ccaacctaga tatcattggc cagaggatag agaacataaa acatgaacat 8340 aagtcaacat ggcattatga tgaggacaat ccatataaaa catgggccta tcatggatca 8400 tatgaggtca agccatcagg atcagcctca tccatggtca atggcgtggt gaaactgctc 8460 accaaaccat gggatgtcat ccccatggtc acacaaatag ccatgactga cactacaccc 8520 tttggacaac agagggtgtt taaagagaaa gttgacacac gcacaccaaa agcaaaacga 8580 ggcacagcac aaatcatgga ggtgacagcc aggtggttat ggggttttct ttctagaaac 8640 aagaaaccaa gaatttgtac aagagaggag ttcacaagaa aagttaggtc aaacgcagcc 8700 attggagcag tgttcgttga tgaaaatcaa tggaactcag caaaagaagc agtggaagat 8760 gaccggttct gggaccttgt gcacagagag agggagcttc acaaacaggg aaaatgtgcc 8820 acgtgtgttt acaacatgat ggggaagaga gagaaaaagc taggagagtt cggaaaggca 8880 aaaggaagtc gtgcaatatg gtacatgtgg ttaggagcac gctttctaga gttcgaagct 8940 cttggtttca tgaacgaaga tcactggttc agcagagaga attcactcag cggagtggaa 9000 ggagaaggac ttcacaaact tggatatata ctcagagaca tatcaaagat tccaggggga 9060 aacatgtatg cagatgacac agccggatgg gatacaagga taacagagga tgacctccag 9120 aatgaggcca aaattactga catcatggaa cccgaacatg ccctactggc taagtcaatc 9180 ttcaagctaa cctaccaaaa taaggtggta agggttcaga gaccagcaaa aaatggaacc 9240 gtgatggatg tcatatccag acgtgaccag agaggaagtg gccaggttgg aacttatggc 9300 ttaaacactt tcaccaacat ggaagcccag ctgataagac aaatggagtc tgagggaatc 9360 ttttcaccca acgaattaga gaccccaaat ttagccgaga gagttctcga ctggctggaa 9420 aaatatggcg tcgaaaggct gaaaagaatg gcaatcagtg gagatgactg cgtagtgaaa 9480 ccaattgatg acaggttcgc gacagcctta acagctctga atgatatggg aaaagtraga 9540 aaagatatac cacaatggga accttcaaaa ggatggaatg attggcaaca agtgcctttt 9600 tgttcacacc acttccacca gctgattatg aaggatggga gggaaatagt ggtgccatgc 9660 cgcaaccaag atgaacttgt gggtagggct agagtatcac aaggtgctgg atggagcctg 9720 agagaaactg catgcctagg caagtcatat gcacaaatgt ggcagctgat gtacttccac 9780 aggagagacc tgagactagc cgctaatgct atctgttcag ccgttccagt tgattgggtc 9840 ccaaccagcc gcaccacctg gtcgatccat gcccatcacc aatggatgac aacagaagac 9900 atgctgtcag tatggaatag ggtttggata gaggaaaacc catggatgga ggacaaaacc 9960 cgtatatcca gttgggaaga tgttccatat ctaggaaaaa gggaagatca atggtgtgga 10020 tctctgatag gcttaacagc aagggccacc tgggccacca acatacaagt ggccataaac 10080 caagtgagaa gactaattgg gaatgagaat tattcagatt acatgacatc aatgaagaga 10140 ttcaagaacg agagtgaacc cgaaggggca ctctgg 10176 <210> 26 <211> 10173 <212> DNA <213> Dengue virus type 2 <400> 26 atgaataacc aacggaaaaa ggcgaaaaac acgcctttca atatgctgaa acgcgagaga 60 aaccgcgtgt cgactgtgca acagctgaca aagagattct cacttggaat gctgcaggga 120 cgaggaccat taaaactgtt catggccctg gtagctttcc ttcgtttcct aacaatccca 180 ccaacagcag ggatattgaa gagatgggga acaattaaaa aatcaaaagc cattaatgtt 240 ttgagagggt tcaggaaaga gatcggaagg atgctgaaca tcttgaatag gagacgcagg 300 tctgcaggca tgatcattat gctgattcca acagtgatgg cgttccatct aaccacacgc 360 aacggagaac cacacatgat cgtcggtata caggagaaag ggaaaagtct tctgttcaaa 420 acagagaatg gtgtgaacat gtgcaccctc atggctatgg accttggtga attgtgtgaa 480 gacacaatca cgtacaaatg tcctcttctc aggcagaatg agccagaaga catagactgt 540 tggtgcaact ccacgtccac gtgggtaacc tatgggacct gtaccactac gggagaacat 600 agaagagaaa aaagatcagt ggcactcgtt ccacatgtgg gaatgggact ggagacgcga 660 accgaaacat ggatgtcatc agaaggggct tggaaacatg cccagagaat tgaaacttgg 720 atcctgagac atccaggctt caccataatg gcagcaatcc tggcatacac catagggacg 780 acacatttcc agagagtcct gattttcatt ctactgacag ctgtcgctcc ttcaatgaca 840 atgcgttgca taggaatatc aaatagagac tttgtagaag gggtttcagg aggaagttgg 900 gttgacatag tcttagaaca tggaagctgt gtgacgacga tggcaaaaaa caaaccaaca 960 ttggattttg aactgataaa aacggaagcc aaacagcctg ccaccctaag gaagtactgc 1020 atagaagcaa agctaaccaa cacaacaaca gaatcccgtt gcccaacaca aggggaaccc 1080 agcctaaaag aagagcagga taagaggttc gtctgcaaac actccatggt agacagagga 1140 tggggaaatg gatgtgggtt atttggaaag ggaggcattg tgacctgtgc tatgttcaaa 1200 tgcaaaaaga acatggaagg gaaaatcgtg caaccagaaa acttggaata caccattgtg 1260 gtaacacctc actcagggga ggagcatgcg gtcggaaatg acacaggaaa acacggcaag 1320 gaaatcaaag taacaccaca gagttccatc acagaagctg aattgacagg ttatggcacc 1380 gtcacgatgg agtgctcccc gagaacaggc ctcgacttca atgagatggt gttgctgcag 1440 atggaaaata aagcttggct ggtgcatagg caatggtttc tagacctgcc attrccatgg 1500 ctgcccggag cggataaaca agagtcaaat tggatacaga aagaaacatt ggtcactttc 1560 aaaaatcccc atgcgaagaa acaggatgtt gttgttttag gatcccaaga aggggccatg 1620 catacagcac tcacaggagc cacagaaatc caaatgtcgt caggaaactt gctcttcact 1680 ggacatctca agtgcaggct gagaatggac aagctacagc ttaaaggaat gtcatactcc 1740 atgtgcacag gaaagtttaa agttgtgaag gaaatagcag aaacacaaca tggaacgata 1800 gtcatcagag tgcaatatga aggggacggc tctccatgta aaatcccttt tgagataatg 1860 gatttggaaa aaagatatgt cttaggccgc ctgatcacag tcaacccaat tgtaacagaa 1920 aaagatagcc cagtcaacat agaagcagaa cctccattcg gagacagcta catcatcata 1980 ggagtagagc cgggacaact gaagctcaac tggttcaaga aaggaagttc tatcggccaa 2040 atgtttgaga caacgatgag aggggcgaag agaatggcca ttttgggtga cacagcctgg 2100 gacttcggat ccctgggagg agtgtttaca tctataggaa aagctctcca ccaagtcttt 2160 ggagcaatct atggagctgc cttcagtggg gtttcatgga ccatgaaaat cctcatagga 2220 gtcattatca catggatagg aatgaactca cgcagcacct cactgtctgt gtcactagta 2280 ctggtgggaa ttgtgacact gtatttggga gtcatggtgc aggccgatag tggttgcgtc 2340 gtgagctgga aaaacaaaga actgaaatgt ggcagtggga ttttcatcac agacaacgtg 2400 cacacatgga cagaacaata caagttccaa ccagaatccc cttcaaaact agcttcagct 2460 atccagaaag ctcaagaaga gggaatttgt gggatccgct cggtaacaag attggagaac 2520 ctaatgtgga aacaaataac accagaattg aatcacattc tagcagaaaa tgaggtgaag 2580 ttgactatca tgacaggaga catcaaagga atcatgcagg caggaaaacg atctttgcgg 2640 cctcagccca ctgagctaaa gtattcatgg aaaacatggg gtaaagcaaa aatgctctct 2700 acagaatctt ataaccaaac ctttctcatt gatggccccg agacggcaga gtgccccaac 2760 acaaatagag cttggaactc gttggaggtt gaagactacg gctttggagt gttcaccacc 2820 aatatatggc taaaattgaa agaaaaacag gatgcattct gcgactcaaa actcatgtcg 2880 gcggccataa aagacaacag agccgtccat gccgacatgg gttactggat agaaagtgca 2940 ctcaatgaca catggaagat agagaaagcc tctttcattg aagtaaaaaa ctgccactgg 3000 ccaaaatcac acaccctctg gagcaatgga gtgctagaaa gtgagatgat aatcccaaag 3060 aatctcgctg gaccagtgtc tcaacacaac tatagaccag gctaccatac acaagtagca 3120 ggaccatggc acctaggtaa gcttgagatg gactttgatt tctgcgatgg aaccacagta 3180 atagtgactg aggactgcgg aaatagagga ccctctttga gaacaactac tgcctctgga 3240 aaactcataa cggaatggtg ctgccgatct tgcacattac cacccctaag atacagaggt 3300 gagaatgggt gctggtacgg gatggaaatc agaccattga aagagaaaga agaaaatctg 3360 gttaaytcct tggtcacagc cggacatggg caggttgaca atttttcact gggagtcttr 3420 ggaatggcat tgttcctgga ggaaatgctt aggacccgag taggaacgaa acatgcaata 3480 ctactagttg cagtttcttt tgtgacattg atcacaggga acatgtcctt caaagaccta 3540 ggaagagtgg tggttatggt aggcgctacc atggcggatg acataggtat gggcgtgact 3600 tatcttgccc tactagcagc cttcaaagtt agaccaactt ttgcagctgg actactcttg 3660 agaaagctga cctccaagga attgatgatg actaccatag gaattgtact cctctcccaa 3720 agcactatac cagagaccgt tcttgaattg actgacgcgt tagccttagg catgatggtc 3780 cttaaaatag taagaaacat ggaaaagtat caattagcag tgactatcat ggctatcttg 3840 tgcgtcccaa acgcagtgat attgcaaaat gcatggaaag tgagttgcac aatactggca 3900 gtggtgtccg tttccccact gtttttraca tcctcacagc aaaaaacgga ttggatacca 3960 ttagcattga caatcaaggg tctcaatcca acagccattt ttctaacaac cctctcaaga 4020 accaacaaga aaagaagctg gccactaaat gaggctatca tggcagtcgg aatggtgagc 4080 attttagcca gttccctcct aaaaaatgat attcccatga caggaccact agtggctgga 4140 gggctcctca ctgtgtgcta cgtgctyact ggacgatcgg ccgacttgga actggagaga 4200 gcaaccgatg tcaaatggga agaccaggca gagatatcag gaagcagtcc aatcctgtca 4260 ataacaatat cagaagatgg tagcatgtcg ataaaaaatg aagaggaaga acaaacactg 4320 accatactca ttagaacagg attgctggtg atctcaggac tttttcctat atcaatacca 4380 gycacggcag cagcatggta cctgtgggaa gtgaagaaac aacgggccgg agtattgtgg 4440 gacgttcctt cacctccacc catgggaaag gctgaactgg aagatggagc ctacagaatc 4500 aagcaaaaag ggattcttgg gtattctcag atcggagctg gagtttacaa agaaggaaca 4560 tttcatacaa tgtggcatgt cacacgtggc gctgttctaa tgcataaagg aaagaggatt 4620 gaaccttcat gggcggacgt caaaaaagat ctaatatcat atggaggagg ctggaagtta 4680 gaaggagaat ggaaggaagg agaagaagtc caggtgctgg cactggagcc tggaaaaaat 4740 ccaagagccg tccaaacaaa acctggtctt ttcaaaacca acaccggaac aataggtgcc 4800 gtatctctgg acttttcccc tggaacgtca ggatctccaa tcatcgacaa aaaaggaaaa 4860 gttgtgggtc tttatggcaa tggtgttgtc acaaggagtg gagcatatgt gagtgctata 4920 gcccaaactg aaaaaagcat tgaagayaac cccgagatca aagatgacat tttccgaaag 4980 agaagactga ccatcatgga cctccaccca ggagcgggaa agacgaaaag ataccttcca 5040 gccatagtca gagaagccat aaaacggggt ttgagaacat taatcttggc ccccactaga 5100 gttgtggcag ctgaaatgga ggaagctctt agaggacttc caataagata ccaaacccca 5160 gccatcagag ctgagcacac cgggcgggaa attgtggatc taatgtgtca tgccacattt 5220 accatgaggc tgctgtcacc agttagagtg ccaaactata acttgattat catggacgaa 5280 gcccatttca cagacccagc aagtatagca gctagaggat acatctcaac ccgagtggag 5340 atgggtgagg cagctgggat tttcatgaca gccactcccc cgggaagtag agatccattt 5400 cctcagagca atgcaccaat catagatgaa gaaagagaaa tccctgaacg ttcatggaat 5460 tctggacatg agtgggtcac ggattttaaa gggaagactg tttggttcgt tccaagcata 5520 aaagcaggaa atgatatagc agcttgcctg agaaaaaatg gaaagaaagt aatacaactt 5580 agtaggaaga cttttgattc tgagtatgtc aagactagaa ccaatgattg ggatttcgtg 5640 gttacaactg acatttcaga aatgggtgcc aatttcaaag ctgagagggt tatagacccc 5700 agacgctgca tgaaaccagt catactaaca gatggtgagg agcgggtgat tctggcagga 5760 cctatgccag tgacccactc tagcgcagca caaagaagag ggagaatagg aagaaatcca 5820 aaaaatgaaa atgaccagta catatacatg ggagaacctt tggaaaatga tgaagactgt 5880 gcacactgga aagaagccaa aatgctccta gataacatta acacaccaga aggaatcatt 5940 cctagtatgt ttgaaccaga gcgtgaaaaa gtggatgcca ttgatggcga ataccgtttg 6000 agaggagaag caaggaaaac ctttgtggac ttaatgagga gaggagactt accagtctgg 6060 ttggcttaca aagtggcagc tgaaggcatc aactatgcag acagaaggtg gtgttttgat 6120 ggaattaaga acaaccaaat cctggaagar aatgtggaag ttgaaatctg gacaaaagaa 6180 ggggaaagga agaaattgaa acccagatgg ttggatgcta ggatctactc tgacccactg 6240 gcgctaaagg aattcaaaga atttgcagcc ggaagaaagt ctctgaccct gaacctaatc 6300 acagaaatgg gtaggcttcc aacattcatg actcagaaga cgagagacgc actggacaac 6360 ctagcagtgc tgcacacggc tgaagcaggt ggaagggcgt acaatcatgc tctcagtgaa 6420 ctgccggaga ccctagagac actgctttta ctgacacttc tggctacagt cacaggaggg 6480 atctttttat tcttgatgag cgggaggggc atagggaaga tgaccctggg aatgtgctgc 6540 ataatcacgg ctagcatcct tctatggtrc gcacaaatac agccacactg gatagcagct 6600 tcaataatac tggagttttt tctcatagtt ttgcttattc cagaaccaga aaaacagaga 6660 acaccccaag acaaccaatt gacctacgtt gtcatagcca tcctcacagt ggtggccgca 6720 accatggcaa acgagatggg tttcctagaa aaaacgaaga aggatcttgg attaggaagc 6780 attgcaaccc agcaacccga gagcaacatc ctggacatag acctacgtcc cgcatcagct 6840 tggacgctgt atgccgtggc tacaacattc gttacaccaa tgctgagaca tagcattgag 6900 aattcctcag tgaatgtatc cctaacagct atagccaacc aagcaacagt gttaatgggt 6960 ctagggaaag gatggccatt gtcaaagatg gacatcggag ttccccttct cgccattgga 7020 tgttactcac aagtcaatcy cataactctt acagcagctc tcctcttatt ggtagcgcat 7080 tacgccatca ttgggccagg acttcaagca aaagcaacca gagaagccca gaaaagagca 7140 gcggcgggca tcatgaaaaa cccaaccgtc gatggaataa cagtgattga tctagatcca 7200 ataccttatg atccaaagtt tgaaaagcag ttgggacaag tratgctact aatcctctgc 7260 gtgactcaag tattgatgat gaggactaca tgggctctgt gtgaggccyt aaccttagct 7320 acagggccca tctccacact gtgggaagga aatccaggaa ggttctggaa cactaccatt 7380 gcggtgtcaa tggctaacat ctttagaggg agttacctgg ctggagctgg acttctcttt 7440 tccattatga agaacacaac caacacaaga aggggaactg gcaacatagg agagacgctt 7500 ggagagaaat ggaaaagccg attgaacgca ctgggaaaaa gtgaattcca gatctacaag 7560 aaaagtggaa tccaggaagt ggatagaacc ttagcaaaag aaggcatcaa aagaggagaa 7620 acggaccatc acgctgtgtc gcgaggctca gcaaaactga gatggttcgt tgagagaaac 7680 atggtcacac cagaagggaa ggtagtggac ctcggttgtg gcagaggagg ctggtcatac 7740 tattgtggag gactaaaaaa cgtaagagaa gtgaaaggcc taacaaaagg aggaccagga 7800 cacgaagaac ccatccctat gtcaacatat gggtggaatc tggtgcgtct tcagagtgga 7860 gttgacgtct tcttcattcc gccagaaaag tgtgacacat tgttgtgtga cataggggag 7920 tcatcaccaa atcccacagt ggaagcagga cgaacactca gagtccttaa cttagtagaa 7980 aactggttga acaacaacac tcaattttgc ataaaagttc tcaacccata tatgccctca 8040 gttatagaaa aaatggaagc actgcaaagg aaatatggag gagccttagt gagaaatcca 8100 ctctcacgga actccacaca tgagatgtac tgggtatcca atgcttctgg gaacatagtg 8160 tcatcagtga acatgatttc aaggatgttg atcaatagat tcacaatgag acacaagaaa 8220 gccacctacg agccggatgt tgacctcgga agcggaaccc gaaacattgg agttgaaagt 8280 gaaataccaa acctagacat aattgggaaa agaatagaaa aaataaagca agagcatgaa 8340 acatcatggc actacgacca agaccaccca tacaaaacgt gggcatacca tggtagctat 8400 gaaacaaaac agactggatc agcatcatcc atggtcaacg gagtggtcag gctgctgaca 8460 aaaccttggg acgttctccc cayggtgaca cagatggcaa tgacagacac gactccattt 8520 ggacaacagc gtgtttttaa agaaaaagtg gacacgagaa cccaagaacc gaaagaaggc 8580 acgaagaaac taatgagaat cacagcagag tggctgtgga aagaattagg gaagaaaaag 8640 acacctagga tgtgcaccag agaagaattc acaagaaagg tgagaagcaa tgcagccttg 8700 ggggccatat tcactgatga gaacaagtgg aagtcggcac gtgaggctgt tgaagatagt 8760 aggttttggg agctggttga caaggaaagg aatctccatc ttgaaggaaa gtgtgagaca 8820 tgtgtgtaca acatgatggg aaaaagagaa aagaagctag gggaattcgg caaggcaaaa 8880 ggcagcagag ccatatggta catgtggctt ggagctcgct tcctggagtt tgaagcccta 8940 ggattcctaa atgaagatca ctggttctct agagagaact ccctgagtgg agtggaagga 9000 gaagggctgc acaagctagg ttacattcta agagacgtga gcaagaaaga gggaggarcc 9060 atgtacgccg acgacacagc aggatgggac acaagaatca cactagaaga cctaaaaaat 9120 gaagaaatgg taacaaacca catggaagga gaacacaaga aattggccga ggctattttc 9180 aaactaacgt atcaaaacaa ggtggtgcgt gtgcaaagac caacaccaag aggcacagta 9240 atggacatca tatcgagaag agaccaaaga ggtagtgggc aagttggtac atatggactc 9300 aatactttca ccaatatgga agcccaatta atcagacaga tggagggaga aggagtcttt 9360 aaaaacatcc agcacctgac agtcacagaa gaagttgccg tgcaaaactg gttggcaaga 9420 gtggggcgcg aaaggttgtc aagaatggcc atcagtggag atgattgtgt tgtgaaacct 9480 ttagatgaca ggttcgcaaa cgctttgaca gctctaaatg acatgggaaa gattagaaaa 9540 gatatacaac aatgggaacc ttcaaaagga tggaacgatt ggacacaagt gcccttctgt 9600 tcacaccatt tccatgagct aatcatgaag gatggtcgtg tacttgttgt tccatgtaga 9660 aaccaagatg aattgattgg cagagcccga atctcccaag gagcagggtg gtctttgcgg 9720 gagacggcct gtttggggaa gtcttacgcc caaatgtgga gcttgatgta tttccacagg 9780 cgtgacctca ggctggcggc aaatgccatt tgctcggcag tgccatcaca ctgggttcca 9840 acaagtcgaa caacctggtc catacacgcc aaacatgaat ggatgacaac ggaagacatg 9900 ctgacagtct ggaacagggt gtggattcga gaaaacccat ggatggaaga caaaacccca 9960 gtggaatcat gggaggaaat cccatacttg ggaaaaagag aagaccaatg gtgcggctca 10020 ttgattgggt taacaagcag ggccacatgg gcaaagaaca tccaagcagc aataaatcag 10080 gttagatctc ttataggcaa tgaggaatac acagattaca tgccatccat gaaaagattt 10140 agaagagaag aggaagaagc aggagtcctg tgg 10173 <210> 27 <211> 10170 <212> DNA <213> Dengue virus type 3 <400> 27 atgaacaacc aacggaaaaa gacgggaaaa ccgtctatca atatgctgaa acgcgtgaga 60 aaccgtgtgt caactggatc acagttggcg aagagattct caagaggatt gctgaacggc 120 caaggaccaa tgaaattggt tatggcattt atagctttcc tcagatttct agccattcca 180 ccgacagcag gagtcttggc tagatggggt acctttaaga agtcgggggc tattaaggtc 240 ttaaaaggct tcaagaagga gatctcaaac atgctgagca ttatcaacaa acggaaaaag 300 acatcgctct gtctcatgat gatgttacca gcaacacttg ctttccactt aacttcacga 360 gatggagagc cgcgcatgat tgtggggaag aatgaaagag gaaaatccct acttttcaag 420 acagcctctg gaatcaacat gtgcacactc atagctatgg atctgggaga gatgtgtgat 480 gacacggtca cttacaaatg cccccacatt accgaagtgg agcctgaaga cattgactgc 540 tggtgcaacc ttacatcgac atgggtgact tatggaacat gcaatcaagc tggagagcat 600 agacgcgata agagatcagt ggcgttagct ccccatgttg gcatgggact ggacacacgc 660 actcaaacct ggatgtcggc tgaaggagct tggagacaag tcgagaaggt agagacatgg 720 gcccttaggc acccagggtt taccatacta gccctatttc ttgcccatta cataggcact 780 tccttgaccc agaaagtggt tatttttata ctattaatgc tggttacccc atccatgaca 840 atgagatgtg taggagtagg aaacagagat tttgtggaag gcctatcggg agctacgtgg 900 gttgacgtgg tgctcgagca cggtgggtgt gtgactacca tggctaagaa caagcccacg 960 ctggacatag agcttcagaa gaccgaggcc acccaactgg cgaccctaag gaagctatgc 1020 attgagggaa aaattaccaa cataacaacc gactcaagat gtcccaccca aggggaagcg 1080 attttacctg aggagcagga ccagaactac gtgtgtaagc atacatacgt ggacagaggc 1140 tggggaaacg gttgtggttt gtttggcaag ggaagcttgg tgacatgcgc gaaatttcaa 1200 tgtttagaat caatagaggg aaaagtggtg caacatgaga acctcaaata caccgtcatc 1260 atcacagtgc acacaggaga ccaacaccag gtgggaaatg aaacgcaggg agtcacggct 1320 gagataacac cccaggcatc aaccgctgaa gccattttac ctgaatatgg aaccctcggg 1380 ctagaatgct caccacggac aggtttggat ttcaatgaaa tgatctcatt gacaatgaag 1440 aacaaagcat ggatggtaca tagacaatgg ttctttgact tacccctacc atggacatca 1500 ggagctacag cagaaacacc aacttggaac aggaaagagc ttcttgtgac atttaaaaat 1560 gcacatgcaa aaaagcaaga agtagttgtt cttggatcac aagagggagc aatgcataca 1620 gcactgacag gagctacaga gatccaaacc tcaggaggca caagtatctt tgcggggcac 1680 ttaaaatgta gactcaagat ggacaaattg gaactcaaag ggatgagcta tgcaatgtgc 1740 ttgggtagct ttgtgttgaa gaaagaagtc tccgaaacgc agcatgggac aatactcatt 1800 aaggttgagt acaaagggga agatgcaccc tgcaagattc ctttctccac ggaggatgga 1860 caaggaaaag ctcacaatgg cagactgatc acagccaatc cagtggtgac caagaaggag 1920 gagcctgtca acattgaggc tgaacctcct tttggagaaa gtaacatagt aattggaatt 1980 ggagacaaag ccctgaaaat caactggtac aagaagggaa gctcgattgg gaagatgttc 2040 gaggctactg ccagaggtgt aaggcgcatg gccatcttgg gagacacagc ctgggacttt 2100 ggatcagtgg gtggtgtttt gaattcatta gggaaaatgg tccaccaaat atttgggagt 2160 gcttacacag ccctatttgg tggagtctcc tggatgatga aaattggaat aggtgtcctc 2220 ttaacctgga tagggttgaa ctcaaaaaat acttctatgt cattttcatg catcgcgata 2280 ggaatcatta yactctatct gggagccgtg gtgcaagctg acatggggtg tgtcataaac 2340 tggaaaggca aagaactcaa atgtggaagt ggaattttcg tcactaatga ggtccacacc 2400 tggacagagc aatacaaatt tcaagcagac tcccccaaga gactggcaac agccattgca 2460 ggcgcttggg aaaatggagt gtgcggaatt aggtcaacaa ccagaatgga gaacctcttg 2520 tggaagcaaa tagccaatga actgaattac atattatggg aaaacaacat taaattaacg 2580 gtagttgtag gcgacataac tggggtctta gagcaaggga aaagaacact aacaccacaa 2640 cccatggagc taaaatattc ttggaaaaca tggggaaagg caaaaatagt gacagctgaa 2700 acacaaaatt cctctttcat aatagatggg ccaagcacac cggagtgtcc aagtgcctca 2760 agagcatgga atgtgtggga ggtggaggat tacgggttcg gagttttcac aaccaacata 2820 tggctgaaac tccgagaggt gtacacccaa ctatgtgacc ataggctaat gtcggcagcc 2880 gtcaaggatg agagggctgt acatgccgac atgggctatt ggatagaaag ccaaaagaat 2940 gggagttgga agctagaaaa agcatccttc atagaggtga aaacctgcac atggccaaaa 3000 tcacacactc tctggagcaa tggtgtgcta gagagtgaca tgattatccc aaagagtcta 3060 gctggtccca tttcgcaaca caaccacagg cccgggtacc acacccaaac ggcaggaccc 3120 tggcacttag gaaaattgga gctggacttc aactattgtg aaggaacaac agttgtcatc 3180 tcagaaaact gtgggacaag aggcccatca ttgagaacaa caacggtgtc agggaagttg 3240 atacacgaat ggtgctgccg ctcgtgcaca cttcctcccc tacgatacat gggagaagac 3300 ggctgctggt atggcatgga aatcagaccc attaatgaga aagaagagaa tatggtaaag 3360 tctctagcct cagcagggag tggaaaggtg gacaacttca caatgggtgt cttgtgtttg 3420 gcaatcctct ttgaagaggt gatgagagga aaatttggga aaaaacacat gattgcaggg 3480 gttctcttca cgtttgtgct cctcctctca gggcaaataa catggagaga catggcgcac 3540 acactcataa tgattgggtc caacgcctct gacagaatgg ggatgggcgt cacttaccta 3600 gctctaattg caacatttaa aattcagcca ctcctggctt tgggattctt cctgaggaaa 3660 ctgacatcta gagaaaattt attgctggga gttgggttgg ccatggcaac aacgttacga 3720 ctgccagagg acattgaaca gatggcgaat ggaattgctt tggggctcat ggctcttaaa 3780 ctgataacac aatttgaaac ataccaacta tggacggcat tagtttccct aacgtgttca 3840 aatacaattt tcacgttgac tgttgcctgg agaacagcca ctctgatttt agccggaatt 3900 tcgcttttgc cagtgtgcca gtcttcgagc atgaggaaaa cagattggct cccaatgact 3960 gtggcagcta tgggagctca acccctacca ctttttattt tcagtctgaa agatacactc 4020 aaaaggagaa gctggccact gaatgagggg gtgatggcag ttggacttgt gagcattcta 4080 gctagttctc tccttaggaa tgatgtgcct atggctggac cattagtggc tgggggcttg 4140 ctgatagcgt gctacgtcat aactggcacg tcagcagacc tcactgtaga aaaggcagca 4200 gatgtaacat gggaggaaga ggccgagcaa acaggagtgt cccacaattt aatggtcaca 4260 gttgatgatg atggaacaat gagaataaaa gatgacgaga ctgagaacat cttaacagtg 4320 cttttaaaaa cagcactact aatagtatca ggcatctttc catactccat acccgcaaca 4380 ctgttggtct ggcatacttg gcaaaagcaa acccaaagat ccggcgtcct atgggacgta 4440 cccagccccc cagagacaca gaaagcggaa ctggaagaag gggtctatag gatcaaacag 4500 caaggaattt ttgggaaaac ccaagtgggg gttggagtac agaaagaagg agttttccac 4560 accatgtggc atgtcacaag aggggcagtg ttgacacaca atgggaaaag actggaacca 4620 aactgggcta gcgtgaaaaa agatctgatt tcatacggag gaggatggag attgagtgca 4680 caatggaaaa agggggagga ggtgcaggtt attgccgtag agcctgggaa gaacccaaag 4740 aactttcaaa ccatgccagg catttttcag acaacaacag gggaaatagg agcaattgca 4800 ctggatttca agcctggaac ttcaggatct cccatcataa acagagaggg aaaggtagtg 4860 ggactgtatg gcaatggagt ggttacaaag aatggaggct atgtcagtgg aatagcgcaa 4920 acaaatgcag aaccagatgg accgacacca gagttggaag aagagatgtt caaaaagcga 4980 aatctaacca taatggatct ccatcctggg tcaggaaaga cgcggaaata tcttccagct 5040 attgttagag aggcaatcaa gagacgctta aggactctaa ttttggcacc aacaagggta 5100 gttgcagctg agatggaaga agcattgaaa gggctcccaa taaggtatca aacaactgca 5160 acaaaatctg aacacacagg aagagagatt gttgatctaa tgtgtcacgc aacgttcaca 5220 atgcgcttgc tgtcaccagt cagggttcca aactacaact tgataataat ggatgaggcc 5280 catttcacag acccagccag tatagcggct agagggtaca tatcaactcg tgtaggaatg 5340 ggagaggcag ccgcaatttt catgacagca acaccccctg gaacagctga tgcctttcct 5400 cagagcaacg ctccaattca agatgaagag agagacatac cggaacgctc atggaattca 5460 ggcaatgaat ggattactga ctttgttggg aagacagtgt ggtttgtccc tagcatcaaa 5520 gccggaaatg acatagcaaa ctgcttgcgg aaaaatggaa aaaaggttat tcaactcagc 5580 aggaagacct ttgacacaga atatcaaaag accaaactga atgattggga ctttgtggtg 5640 acaacagaca tttcagaaat gggagccaat ttcaaagcag atagagtgat cgacccaaga 5700 agatgtctca agccggtgat tttgacagat ggacccgagc gggtgatcct ggctggacca 5760 atgccagtca ccgtagcgag cgctgcgcaa aggagaggga gagttggcag gaacccacaa 5820 aaagaaaatg accagtacat attcatgggc cagcctctca acaatgatga agaccatgct 5880 cactggacag aagcaaaaat gctgctggac aacatcaaca caccagaagg gattatacca 5940 gctctctttg aaccagaaag ggagaagtca gccgccatag acggcgaata ccgcctgaag 6000 ggtgagtcca ggaagacttt cgtggaactc atgaggaggg gtgacctccc agtttggcta 6060 gcccataaag tagcatcaga agggatcaaa tatacagata gaaaatggtg ctttgatgga 6120 gaacgtaata atcaaatttt agaggagaat atggatgtgg aaatctggac aaaggaagga 6180 gaaaagaaaa aactgagacc taggtggctt gatgcccgca cttattcaga tcctttagca 6240 ctcaaagaat tcaaggattt tgcagctggc agaaagtcaa tcgcccttga tcttgtgaca 6300 gaaataggaa gagtgccttc acacttagcc cacagaacga gaaacgccct ggataatttg 6360 gtgatgctgc acacgtcaga acatggcggt agggcctaca ggcatgcagt ggaggaacta 6420 ccagaaacga tggaaacact cttactcctg ggactgatga tcttgttaac aggtggagca 6480 atgctcttct tgatatcagg taaagggatt ggaaagactt caataggact catttgtgta 6540 attgcttcca gcggcatgtt atggatggct gatgtcccac tccaatggat cgcatcggct 6600 atagtcctgg agttttttat gatggtgttg ctcataccag aaccagaaaa gcagagaact 6660 ccccaagaca accaactcgc atatgtcgtg ataggcatac ttacattggc tgcaatagta 6720 gcggccaatg aaatgggact gttggaaact acaaagagag atttaggaat gtctaaagaa 6780 ccaggtgttg tttctccaac cagctatttg gatgtggact tgcacccagc atcagcctgg 6840 acattgtacg ccgtggccac aacagtaata acaccaatgt tgagacacac catagagaat 6900 tccacagcaa atgtgtctct ggcagccata gctaaccagg cagtggtcct gatgggttta 6960 gacaaaggat ggccgatatc gaaaatggac ttgggcgtac cactattggc actgggttgc 7020 tattcacaag tgaacccact aactcttgca gcggcagtac ttttgctagt cacacattat 7080 gcaattatag gtccaggatt gcaggcaaaa gccacccgtg aagctcagaa aaggacagct 7140 gctggaataa tgaagaatcc aacggtggat ggaataatga caatagacct agatcctgta 7200 atatatgatt caaaatttga aaagcaacta ggacaggtca tgctcctggt tctgtgtgca 7260 gtccaacttt tattgatgag aacatcatgg gccttgtgtg aagttytaac cctagccaca 7320 ggaccaataa caacactctg ggaaggatca cctgggaagt tctggaacac cacgatagct 7380 gtttccatgg cgaacatctt tagagggagc tatttagcag gagctgggct tgctttttct 7440 atcatgaaat cagttggaac aggaaagaga ggaacagggt cacaaggtga aaccttagga 7500 gaaaagtgga aaaagaaatt aaatcagtta tcccggaaag agtttgacct ttacaagaaa 7560 tccggaatca ccgaagtgga tagaacagaa gccaaagaag ggttaaaaag aggagaaata 7620 acacaccatg ccgtgtccag aggcagcgca aaacttcaat ggttcgtgga gagaaacatg 7680 gtcattcctg aaggaagagt catagaccta ggctgtggaa gaggaggctg gtcatattac 7740 tgtgcaggac tgaaaaaagt tacagaagtg cgaggataca caaaaggcgg cccaggacac 7800 gaagaaccag tacctatgtc tacatacgga tggaacatag tcaagttaat gagtggaaag 7860 gatgtttttt atctgccacc tgaaaagtgt gataccctat tgtgtgacat tggagaatct 7920 tcaccaagcc caacagtgga agaaagcaga accataagag ttttgaagat ggttgaacca 7980 tggctaaaga acaaccagtt ttgcattaaa gtattgaacc catacatgcc aactgtgatt 8040 gagcacttag aaagactaca aaggaaacat ggaggaatgc ttgtgagaaa tccactctca 8100 cgaaactcca cgcacgaaat gtattggata tccaatggta caggcaatat cgtctcttca 8160 gtcaacatgg tatccagatt gctactgaac agattcacaa tgacacacag gagacccacc 8220 atagagaaag atgtggatct aggagcagga acccgacatg tcaatgcgga accagaaaca 8280 cccaacatgg atgtcattgg ggaaagaata aaaaggatca aagaggagca tagttcaaca 8340 tggcactatg atgatgaaaa tccttacaaa acgtgggctt accatggatc ctatgaagta 8400 aaagccacag gctcagcctc ctccatgata aatggagtcg tgaaactcct cacaaaacca 8460 tgggatgtgg tgcccatggt gacacagatg gcaatgacag atacaactcc attcggccag 8520 caaagagttt ttaaagagaa agtggacacc aggacaccta ggcccatgcc aggaacaaga 8580 aaggttatgg agatcacagc ggagtggctt tggaggaccc tgggaaggaa caaaagaccc 8640 agattatgca caagggagga attcacaaag aaggtcagaa ccaacgcagc tatgggcgct 8700 gtcttcacag aagagaacca atgggacagt gcgagagctg ctgttgagga cgaagaattt 8760 tggaaacttg tggacagaga acgtgaactc cacaaactgg gcaagtgtgg aagctgcgtt 8820 tacaacatga tgggcaagag agagaaaaaa cttggagagt ttggtaaagc aaaaggcagt 8880 agggctatat ggtacatgtg gttgggagcc aggtaccttg agttcgaggc gctcggattc 8940 ctcaatgaag accactggtt ctcgcgtgaa aactcttaca gtggagtaga aggagaagga 9000 ctgcacaagc tgggatacat cttgagagat atttccaaga tacccggagg agccatgtat 9060 gctgatgaca cagccggttg ggacacaaga ataacagaag atgacctgca caatgaggaa 9120 aaaatcacac agcagatgga ccctgaacac aggcagctag cgaacgctat attcaagctc 9180 acataccaaa acaaagtggt caaagtccaa cgaccaactc caaagggcac ggtaatggac 9240 atcatatcta ggaaagacca aagaggcagt ggacaggtgg gaacttatgg tctgaacaca 9300 ttcaccaaca tggaagccca gctaatcaga caaatggaag gagaaggcgt gttgtcaaag 9360 gcagacctcg agaaccccca tccgctagag aagaaaatta cacaatggtt ggaaactaaa 9420 ggagtggaaa ggttaaaaag aatggccatc agcggggatg attgcgttgt gaaaccaatc 9480 gacgacagat tcgccaatgc cctgcttgcc ctgaacgata tgggaaaggt tagaaaggac 9540 atacctcaat ggcagccatc aaagggatgg catgattggc aacaggtccc cttctgctcc 9600 caccactttc atgaattgat catgaaagat ggaagaaagt tggtagttcc ctgcagaccc 9660 caggacgaac taataggaag agcgagaatc tcccaaggag caggatggag ccttagagaa 9720 actgcatgtc tagggaaagc ctacgctcaa atgtgggctc tcatgtattt tcacagaaga 9780 gatcttagac tagcatccaa cgccatatgt tcagcagtac cagtccactg ggtccccacg 9840 agcagaacga catggtctat tcatgctcac catcagtgga tgactacaga agacatgctt 9900 actgtctgga acagggtgtg gatagaggac aatccatgga tggaagacaa aactccagtc 9960 acaacgtggg aagatgttcc atatctaggg aagagagaag accaatggtg cggatcactc 10020 ataggtctca cttccagagc aacctgggcc cagaacatac tcacagcaat ccaacaggtg 10080 agaagcctca taggcaatga agagtttctg gactacatgc cttcgatgaa gagattcagg 10140 aaggaggagg agtcagaggg agccatttgg 10170 <210> 28 <211> 10161 <212> DNA <213> Dengue virus type 4 <400> 28 atgaaccaac gaaaaaaggt ggttagacca cctttcaata tgctgaaacg cgagagaaac 60 cgcgtatcaa cccctcaagg gttggtgaag agattctcaa ccggactttt ttctgggaaa 120 ggacccttac ggatggtgct agcattcatc acgtttttgc gagtcctttc catcccacca 180 acagcaggga ttctgaaaag atggggacag ttgaagaaaa ataaggccat caggatactg 240 attggattca ggaaggagat aggccgcatg ctgaacatct tgaacgggag aaaaaggtca 300 acgataacat tgctgtgctt gattcccacc gtaatggcgt ttcacttgtc aacaagagat 360 ggcgaacccc tcatgatagt ggcaaaacat gaaaggggga gacctctctt gtttaagaca 420 acagagggga tcaacaaatg cactctcatt gccatggact tgggtgaaat gtgtgaggac 480 actgtcacgt ataaatgccc cttactggtc aataccgaac ctgaagacat tgattgctgg 540 tgcaatctca cgtctacctg ggtcatgtat gggacatgca cccagagcgg agaacggaga 600 cgagagaagc gctcagtagc tttaacacca cattcaggaa tgggattgga aacaagagct 660 gagacrtgga tgtcatcgga aggggcttgg aagcatgctc agagagtaga gagctggata 720 ctcagaaacc caggattcgc gctcttggca ggatttatgg cttatatgat tgggcaaaca 780 ggaatccagc gaactgtctt ctttgtccta atgatgctgg tcgccccatc ctacggaatg 840 cgatgcgtag gagtaggaaa cagagacttt gtggaaggag tctcaggtgg agcatgggtc 900 gatctggtgc tagaacatgg aggatgcgtc acaaccatgg cccagggaaa accaaccttg 960 gattttgaac tgactaagac aacagccaag gaagtggctc tgttaagaac ctattgcatt 1020 gaagcctcaa tatcaaacat aaccacggca acaagatgtc caacgcaagg agagccttat 1080 ctaaaagagg aacaagacca acagtacatt tgccggagag atgtggtaga cagagggtgg 1140 ggcaatggct gtggcttgtt tggaaaagga ggagttgtga catgtgcgaa gttttcatgt 1200 tcggggaaga taacaggcaa tttggtccaa attgagaacc ttgaatacac agtggttgta 1260 acagtccaca atggagacac ccatgcagta ggaaatgaca catccaatca tggagttaca 1320 gccacgataa ctcccaggtc accatcggtg gaagtcaaat tgccggacta tggagaacta 1380 acactcgatt gtgaacccag gtctggaatt gactttaatg agatgattct gatgaaaatg 1440 aaaaagaaaa catggcttgt gcataagcaa tggtttttgg atctacctct accatggaca 1500 gcaggagcag acacatcaga ggttcactgg aattacaaag agagaatggt gacatttaag 1560 gttcctcatg ccaagagaca ggatgtgaca gtgctgggat ctcaggaagg agccatgcat 1620 tctgccctcg ctggagccac agaagtggac tccggtgatg gaaatcacat gtttgcagga 1680 catctcaagt gcaaagtccg tatggagaaa ttgagaatca agggaatgtc atacacgatg 1740 tgttcaggaa agttctcaat tgacaaagag atggcagaaa cacagcatgg gacaacagtg 1800 gtgaaagtca agtatgaagg tgctggagct ccgtgtaaag tccccataga gataagagat 1860 gtgaacaagg aaaaagtggt tgggcgtatc atctcatcca cccctttggc tgagaatacc 1920 aacagtgcaa ccaacataga gttagaaccc ccctttgggg acagctacat agtgataggt 1980 gttggaaaca gtgcattaac actccattgg ttcaggaaag ggagttccat tggcaagatg 2040 tttgagtcca catacagagg tgcaaaacga atggccattc taggtgaaac agcttgggat 2100 tttggttccg ttggtggact gttcacatca ttgggaaagg ctgtgcacca ggtttttgga 2160 agtgtgtata caaccatgtt tggaggagtc tcatggatga ttagaatcct aattgggttc 2220 ctagtgttgt ggattggcac gaactcaagg aacacttcaa tggctatgac gtgcatagct 2280 gttggaggaa tcactctgtt tctgggcttc acagttcaag cagacatggg ttgtgtggtg 2340 tcatggagtg ggaaagaatt gaagtgtgga agcggaattt ttgtggttga caacgtgcac 2400 acttggacag aacagtacaa atttcaaccg gagtccccag cgagactagc gtctgcaata 2460 ttgaatgccc acaaagatgg ggtctgtgga attagatcaa ccacgaggct ggaaaatgtc 2520 atgtggaagc aaataaccaa cgagctaaat tatgttctct gggaaggagg acatgacctc 2580 actgtagtgg ctggggatgt gaagggggtg ttgaccaaag gcaagagagc actcacaccc 2640 ccagtgaatg atctgaaata ttcatggaag acatggggaa aagcaaaaat cttcacccca 2700 gaagcaagaa atagcacatt tttaatagac ggaccagaca cctccgaatg ccccaatgaa 2760 cgaagagcat ggaactttct tgaggtggaa gactatggat ttggcatgtt cacgaccaac 2820 atatggatga aattccgaga aggaagttca gaagtgtgtg accacaggtt aatgtcagcg 2880 gcaattaaag atcagaaagc tgtgcatgct gacatgggtt attggataga gagctcaaaa 2940 aaccagacct ggcagataga gaaagcatct cttattgaag tgaaaacatg tctgtggccc 3000 aagacccaca cattgtggag caatggagtg ctggaaagcc agatgctcat tccaaaatca 3060 tatgcgggcc ctttttcaca ccacaattac cgccagggct atgccacgca aaccgtgggc 3120 ccatggcact taggcaaatt agagatagac tttggagaat gccccggaac aacagtcgca 3180 attcaggagg attgtgacca tagaggccca tctttgagga ccaccactgc atctggaaaa 3240 ctagtcacgc aatggtgctg ccgctcctgc acgatgcctc ccttaaggtt cttgggagaa 3300 gatgggtgct ggtatgggat ggagattagg cccttgagtg aaaaagaaga gaacatggtc 3360 aaatcacagg taacggccgg acagggcaca tcagaaactt tttctatggg tctgttgtgc 3420 ctgaccttgt ttgtggaaga atgcttgagg agaagagtca ctaggaaaca catgatattg 3480 gttgtggtga tcactctttg tgccatcatc ctaggaggcc tcacatggat ggacttacta 3540 cgagccctca tcatgttggg ggacactatg tctggtagaa taggaggaca gatccaccta 3600 gccatcatgg cagtgttcaa gatgtcacca ggataygtgc tgggtgtgtt tttaaggaaa 3660 ctcacttcaa gagagacagc actaatggta ataggaatgg ccatgacaac ggtgctttca 3720 attccacatg accttatgga actcattgat ggaatatcac tggggctaat tttgctaaaa 3780 atagtgacac attttgacaa cacccaagtg ggaaccttag ccctttcctt gaccttcata 3840 agatcaacaa tgccattggt catggcttgg aggaccatta tggctgtgtt gtttgtggtc 3900 acactcattc ctttgtgcag gacaagctgt cttcaaaaac agtctcattg ggtagaaata 3960 acagcactca tcctaggagc ccaagctctg ccagtgtacc taatgactct tatgaaagga 4020 gcctcaagaa gatcttggcc tcttaacgag ggcataatgg ctgtgggttt ggttagtctc 4080 ttaggaagcg ctcttttaaa gaatgatgtc cctttrgctg gcccaatggt ggcaggaggc 4140 ttacttctgg cggcttacgt gatgagtggt agctcagcag atctgtcact agagaaggcc 4200 gccaatgtgc agtgggatga aatggcagac ataacaggct caagcccaat catagaagtg 4260 aagcaggatg aagatggctc tttctccata cgggacgtcg aggaaaccaa tatgataacc 4320 cttttggtga aactggcact gataacagtg tcaggtctct accccttggc aattccagtc 4380 acaatgacct tatggtacat gtggcaagtg aaaacacaaa gatcaggagc cctgtgggac 4440 gtcccctcac ccgctgccac tcaaaaagcc gcactgtctg aaggagtgta caggatcatg 4500 caaagagggt tatttgggaa aactcaggtt ggagtaggga tacacatgga aggtgtattt 4560 cacacaatgt ggcatgtaac aagaggatca gtgatctgcc atgagactgg gagattggag 4620 ccatcttggg ctgacgtcag gaatgacatg atatcatacg gtgggggatg gagacttgga 4680 gacaaatggg acaaagaaga agatgttcag gtcctcgcca tagaaccagg aaaaaatcct 4740 aaacatgtcc aaacgaaacc cggccttttc aagaccctaa ctggagaaat tggagcagta 4800 acattagatt tcaaacccgg aacgtctggt tctcccatca tcaacaggaa aggaaaagtc 4860 atcggactct atggaaatgg agtagttacc aaatcaggtg attacgtcag tgccataacg 4920 caagccgaaa gaattggaga gccagattat gaagtggatg aggacatttt tcgaaagaaa 4980 agattaacta taatggactt acaccccgga gctggaaaga caaaaagaat tcttccatca 5040 atagtgagag aagccttaaa aaggaggctg cgaaccttga ttttggctcc cacgagagtg 5100 gtggcggccg agatggaaga ggccctacgt ggactgccaa tccgttatca gaccccagct 5160 gtgaaatcag aacacacagg aagagagatt gtagacctca tgtgtcatgc aaccttcaca 5220 acaagacttt tgtcatcaac cagagttcca aattacaacc tcatagtgat ggatgaagca 5280 catttcaccg atccttctag tgtcgcggct agaggataca tctcgaccag ggtggaaatg 5340 ggagaggcag cagccatctt catgaccgca acccctcccg gagcgacaga tccctttccc 5400 cagagcaaca gcccaataga agacatcgag agggaaattc cggaaaggtc atggaacaca 5460 gggttcgact ggataacaga ctaccaaggg aaaactgtgt ggtttgttcc cagcataaaa 5520 gctggaaatg acattgcaaa ttgtttgaga aagtcgggaa agaaagttat ccagttgagt 5580 aggaaaacct ttgatacaga gtatccaaaa acgaaactca cggactggga ttttgtggtc 5640 actacagaca tatctgaaat gggggccaat tttagagctg ggagagtgat agaccctagg 5700 agatgcctca agccagttat cctaacagat gggccagaga gagtcatttt agcaggtcct 5760 attccagtga ctccagcaag cgctgctcag agaagagggc gaataggaag gaacccagca 5820 caagaagacg accaatacgt tttctccgga gacccactaa aaaatgatga agatcatgcc 5880 cactggacag aagcaaagat gctgcttgac aatatctaca ccccagaagg gatcattcca 5940 acattgtttg gtccggaaag ggaaaaaacc caagccattg atggagagtt tcgcctcaga 6000 ggggaacaaa ggaagacttt tgtggaatta atgaggagag gagaccttcc ggtgtggctg 6060 agctataagg tagcttctgc tggcatttct tacaaagatc gggaatggtg cttcacaggg 6120 gaaaggaata accaaatttt agaagaaaac atggaggttg aaatttggac tagagaggga 6180 gaaaagaaaa agctaaggcc aagatggtta gatgcacgtg tatacgctga ccccatggct 6240 ttgaaggatt tyaaggagtt tgctagtgga aggaagagca taactctcga catcctaaca 6300 gagattgcca gtttgccaac ttacctttcc tctagggcca agctcgccct tgataacata 6360 gtcatgctcc acacaacaga aagaggaggg agggcctacc aacacgccct gaacgaactc 6420 ccggagtcac tggaaacact tatgcttgta gctttactag gtgctatgac agcaggtatc 6480 ttcctgtttt tcatgcaagg gaaaggaata gggaaattgt caatgggttt gataaccatt 6540 gcggtggcta gtggcttgtt ctgggtagca gaaattcaac cccagtggat agcggcctca 6600 atcatactag agttttttct catggtactg ttgataccgg aaccagaaaa acaaaggacc 6660 ccacaagaca atcaattgat ctacgtcata ttgaccattc tcaccattat tggtctcata 6720 gcagccaacg agatggggct gattgaaaaa acaaaaacgg attttgggtt ttaccaggta 6780 aaaacagaaa ccaccatcct cgatgtggac ttgagaccag cttcagcatg gacgctctat 6840 gcagtagcca ccacaattct gactcccatg ctgagacaca ccatagaaaa cacgtcggcc 6900 aacctatctc tagcagccat tgccaaccag gcggccgtcc taatggggct tggaaaagga 6960 tggccgctcc acagaatgga cctcggtgtg ccgctgttag caatgggatg ctattctcaa 7020 gtgaacccaa caactttgac agcatcctta gtcatgcttt cagtccatta tgcaataata 7080 ggtccaggat tgcaggcaaa agccacaaga gaggcccaga aaaggacagc tgctgggatc 7140 atgaaaaacc ccacggtgga cgggataaca gtaatagatc tagaaccaat atcctatgac 7200 ccaaaatttg aaaagcaatt agggcaggtc atgctactcg tcttgtgtgc tggacaacta 7260 ctcttgatga gaacaacatg ggctttctgt gaagtcttga ctttggccac aggaccaatc 7320 ttgaccttgt gggagggcaa cccgggaagg ttttggaaca cgaccatagc cgtatccacc 7380 gccaacattt tcaggggaag ttacctggcg ggagctggac tggctttttc actcataaag 7440 aatgtacaaa cccctaggag gggaactggg accacaggag agacactggg agagaagtgg 7500 aagagacagc taaactcatt agacagaaaa gagtttgaag agtataaaag aagtggaata 7560 ctagaagtgg acaggactga agccaagtct gccctgaaag atgggtctaa aatcaagcat 7620 gcagtatcta gagggtccag taagattaga tggattgttg agagagggat ggtaaagcca 7680 aaagggaaag ttgtagatct tggctgtggg agaggaggat ggtcttatta catggcgacg 7740 ctcaagaacg tgactgaagt gaaagggtat acaaaaggag gtccaggaca tgaagaaccg 7800 attcccatgg ctacttatgg ctggaatttg gtcaaactcc attcaggggt tgacgtgttc 7860 tacaaaccca cagagcaagt ggacacyctg ctctgtgata ttggggagtc atcttctaat 7920 ccaacaatag aggaaggaag aacattaaga gttttgaaga tggtggagcc atggctctct 7980 tcaaaacctg aattctgcat caaagtcctt aacccctaca tgccaacagt catagaagag 8040 ctggagaaac tgcagagaaa acatggtggg aaccttgtca gatgcccgct gtccaggaac 8100 tccacccatg agatgtattg ggtgtcagga gcgtcgggaa acattgtgag ctctgtgaac 8160 acaacatcaa agatgttgtt gaacaggttc acaacaaggc ataggaaacc cacttatgag 8220 aaggacgtag atcttggggc aggaacgaga agtgtctcca ctgaaacaga aaaaccagac 8280 atgacaatta ttgggagaag gcttcagcga ttgcaagagg agcacaaaga aacctggcat 8340 tatgatcagg aaaacccata cagaacctgg gcgtatcatg gaagctatga agctccttcg 8400 acaggctctg catcctccat ggtgaacggg gtagtaaaac tgctaacaaa accttgggat 8460 gtggttccaa tggtgaccca gttagccatg acagacacaa ccccttttgg gcaacaaaga 8520 gtgttcaaag agaaggtgga taccagaaca ccacaaccaa aacccggtac acgaatggtt 8580 atgaccacga cagccaattg gctgtgggcc ctccttggga agaagaaaaa tcccagactg 8640 tgcacaaggg aagagttcat ctcaaaagtt agatcaaacg cagccatagg cgcagtcttt 8700 caggaagaac agggatggac atcagccagt gaagctgtga atgacagccg gttttgggaa 8760 ctggttgaca aagaaagggc cctacaccag gaagggaaat gtgaatcgtg tgtctacaac 8820 atgatgggaa aacgtgagaa aaagttagga gagtttggca gagccaaggg aagccgagca 8880 atctggtaca tgtggctggg agcgcggttt ctggaatttg aagccctggg ttttttgaat 8940 gaagatcact ggtttggcag agaaaattca tggagtggag tggaagggga aggtctgcac 9000 agattgggat atatcctgga ggagatagac aagaaggatg gagacctaat gtatgctgat 9060 gacacagcag gctgggacac aagaatcact gaggatgacc ttcaaaatga agaactgatc 9120 acggaacaga tggcccccca ccacaagatc ctagccaaag ccattttcaa actaacctat 9180 caaaacaaag tggtgaaagt cctcagaccc acaccgagag gagcggtgat ggatatcata 9240 tccaggaaag accaaagagg tagtggacaa gttggaacat atggtttgaa cacattcacc 9300 aacatggaag ttcaactcat ccgccaaatg gaagctgaag gagtcatcac acaagatgac 9360 atgcagaacc caaaagggtt gaaagaaaga gttgagaaat ggctgaaaga gtgtggtgtc 9420 gacaggttaa agaggatggc aatcagtgga gacgattgcg tggtgaagcc cctggatgag 9480 aggtttggca cttccctcct cttcttgaac gacatgggaa aggtgaggaa agacattccg 9540 cagtgggaac catctaaggg atggaaaaac tggcaagagg ttcctttttg ctcccaccac 9600 tttcacaaga tcttcatgaa ggatggccgc tcactagttg ttccatgtag aaaccaggat 9660 gaactgatag ggagagccag aatctcgcag ggggctggat ggagcttaag agaaacagcc 9720 tgcctgggca aagcttacgc ccagatgtgg tcgctcatgt acttccacag aagggatctg 9780 cgtttagcct ccatggccat atgctcagca gttccaacgg aatggtttcc aacaagcaga 9840 acaacatggt caatccacgc tcatcatcag tggatgacca ctgaagatat gctcaaagtg 9900 tggaacagag tgtggataga agacaaccct aatatgactg acaagactcc agtccattcg 9960 tgggaagata taccttacct agggaaaaga gaggatttgt ggtgtggatc cctgattgga 10020 ctttcttcca gagccacctg ggcgaagaac attcacacgg ccataaccca ggtcagaaac 10080 ctgatcggaa aagaggaata cgtggattac atgccagtaa tgaaaagata cagcgctcct 10140 tcagagagtg aaggagttct g 10161 <210> 29 <211> 10176 <212> DNA <213> Dengue virus type 1 <400> 29 atgaacaacc aacgaaaaaa gacggctcga ccgtctttca atatgctgaa acgcgcgaga 60 aaccgcgtgt caactgtttc acagttggcg aagagattct caaaaggatt gctctcaggc 120 caaggaccca tgaaattggt gatggctttc atagcattcc taagatttct agccataccc 180 ccaacagcag gaattttggc tagatggggc tcattcaaga agaatggagc gatcaaagtg 240 ctacggggtt tcaagaaaga aatctcaaac atgttgaata taatgaatag aaggaaaaga 300 tctgtgacca tgctccttat gctgatgcct acagccttgg cgttccattt gaccacacga 360 gggggagagc cgcacatgat agtcagcaag caggaaagag gaaagtcact tttgtttaag 420 acctcagcag gtgtcaacat gtgcaccctt atagcgatgg atttgggaga gttatgtgag 480 gacacaatga cttacaaatg ccctcgaatc actgaggcgg aaccagatga cgttgattgt 540 tggtgcaatg ccacagacac atgggtgacc tatggaacat gttcccaaac tggcgagcac 600 cgacgagaca aacgttccgt cgcactggcc ccacacgtgg gacttggttt ggaaacaaga 660 actgaaacgt ggatgtcctc tgaaggcgct tggaaacaga tacaaagagt ggagacttgg 720 gccctgagac acccaggatt cacggtgata gccctttttc tagcacatgc cataggaaca 780 tccatcaccc agaaagggat tattttcatt ttgttaatgc tggtaacacc atccatggcc 840 atgcgatgcg tgggaatagg cagcagggac ttcgtggaag gactgtcagg agcaacttgg 900 gtagatgtgg tactggaaca tggaagttgc gtcaccacca tggcaaaaga caaaccaaca 960 ttggacattg aactcttgaa gacggaagtc acaaaccctg ccgtcctgcg caaactgtgc 1020 attgaagcta aaatatcaaa caccaccacc gactcaagat gtccaacaca aggagaagcc 1080 acactggtgg aagaacaaga cgcgaacttt gtgtgtcgac gaacgtttgt ggacagaggc 1140 tggggcaatg gctgtgggct cttcggaaaa ggaagcctac taacgtgtgc taagttcaag 1200 tgtgtgacaa aactggaagg aaagatagtt caatatgaaa acttgaaata ttcagtaata 1260 gtyaccgtcc acactggaga ccagcatcag gtgggaaatg aaagcacaga acatgggaca 1320 actgcaacta taacacctca agctcctacg acggaaatac agctgaccga ctacggagct 1380 cttacattgg attgttcacc tagaacagga ctagacttta atgaaatggt gttgttgaca 1440 atgraaaaaa ratcatggct agtccacaaa caatggtttc tagacttacc actgccttgg 1500 acctcgggag cctcaacatc acaagagact tggaacagac aagatttgct ggtgacattt 1560 aagacagctc atgcaaagaa gcaggaagta gtcgtactag gatcacaaga aggagcaatg 1620 cacactgcgt tgaccggagc gacagaaatc caaacgtctg gaacgacaac aatttttgca 1680 ggacacttga aatgtagact aaggatggac aaactgactc taaaagggat gtcatatgtg 1740 atgtgcacag gctcattcaa gctagagaaa gaagtggctg agacccagca tggaaccgtt 1800 ctagtgcaga ttaaatacga aggaacagat gcaccatgca agatcccttt ttcgacccaa 1860 gatgaaaaag gagtaaccca gaatgggaga ttgataacag ccaaccccat agtcactgac 1920 aaagaaaaac cagtcaacat tgaggcagaa ccgccttttg gtgagagtta catcgtgata 1980 ggagcaggtg aaaaagcttt gaaactaagc tggttcaaga aaggaagcag catagggaaa 2040 atgtttgagg caactgccag aggagcacga aggatggcca tactgggaga caccgcatgg 2100 gactttggtt ctataggagg agtgttcacg tctgttggaa aattagtaca ccagattttt 2160 ggaactgcat atggagtttt gttcagcggt gtttcctgga ccatgaaaat aggaataggg 2220 gttctgctga catggctagg attaaactca aggaacacgt ccctctcgat gacgtgcatt 2280 gcagttggcc tagtaacact atacctagga gtcatggttc aggcggattc aggatgcgta 2340 attaattgga aaggtagaga actcaaatgt ggaagtggca tttttgtcac caatgaagtt 2400 cacacttgga cagagcaata caaatttcaa gctgactccc caaagagact atcagcagcc 2460 atcgggaagg catgggagga aggtgtgtgt ggaattcgat cagcaactcg tctcgagaac 2520 atcatgtgga agcaaatatc aaatgaactg aaccacatct tacttgaaaa tgacatgaaa 2580 ttcacagtgg ttgtaggaga tgttgctggg atcttggctc aaggaaagaa aatgattagg 2640 ccacaaccca tggaacacaa atactcgtgg aaaagctggg gaaaggctaa aatcataggg 2700 gcagatgtac agaactccac cttcatcatt gacggcccaa acaccccaga atgccctgat 2760 gaccaaagag catggaacat ttgggaagtt gaggactatg gatttggaat tttcacgaca 2820 aacatatggc tgaaattgcg tgactcctac acccaattgt gtgaccaccg gctaatgtca 2880 gctgccatca aggacagcaa ggcagttcac gctgacatgg ggtactggat agaaagtgaa 2940 aagaacgaga cctggaagct ggcaagagcc tcgttcatag aagttaaaac atgtatttgg 3000 ccaaaatccc acactctatg gagcaacgga gttctggaaa gtgaaatgat aattccaaag 3060 atctatggag gaccaatatc tcagcataac tacagaccag gatatttcac acaaacggta 3120 gggccgtggc acctaggcaa gttggaactg gattttgact tgtgtgaggg taccacagtc 3180 gttgtggatg aacattgtgg aaatcgagga ccatctctta ggaccacaac agtcacagga 3240 aagataattc atgaatggtg ttgtagatct tgtacgctac cacccttacg tttcagagga 3300 gaagatgggt gctggtacgg tatggaaatc agaccagtca aggaaaagga agagaatcta 3360 gtcaaatcaa tggtctctgc agggtcaggg gaagtggaca gcttttcact aggactgcta 3420 tgcatatcaa taatgatcga agaggtaatg agatccagat ggagtagaaa aatgctgatg 3480 actggaacac tggctgtgtt cctccttctc ataatgggac aattgacatg gaatgatctg 3540 atcagattat gcatcatggt tggagccaac gcttccgata gaatggggat gggaacgacg 3600 tacctagctc tgatggccac ttttaaaatg agaccaatgt ttgctgtagg gctattattc 3660 cgcagactaa catccagaga agttcttctt ctaacaattg gattgagtct agtggcatct 3720 gtggagttac caaattcctt ggaggagctg ggggatggac ttgcaatggg cattatgatt 3780 ttaaaattat tgactgactt tcaatcacat cagttgtggg ctaccttgct gtccttgaca 3840 tttatcaaaa caacgttttc cttgcactat gcatggaaga caatggctat ggtactgtca 3900 attgtatctc tcttcccctt atgcctgtcc acgacctccc agaaaacaac atggcttccg 3960 gtgctattgg gatctcttgg atgcaaacca ctaaccatgt ttcttatagc agaaaacaaa 4020 atctggggaa ggaaaagttg gcccctcaat gaagggatca tggctgttgg aatagtcagc 4080 atcctactaa gttcactcct caagaatgat gtgccgctag ctgggccact aatagctgga 4140 ggcatgctaa tagcatgtta tgttatatct ggaagctcag ccgacctatc actagagaaa 4200 gcagctgagg tctcctggga agaagaagca gaacactctg gtgcctcaca caacatatta 4260 gtggaggtcc aagatgatgg aaccatgaag ataaaggatg aagagagaga tgacacgcta 4320 accattctcc ttaaagcaac tctgctagca gtttcagggg tgtacccatt atcaatacca 4380 gcgacccttt ttgtgtggta cttttggcag aaaaagaaac agagatctgg agtgttatgg 4440 gacacaccta gccctccaga agtggaaaga gcagttcttg atgatggtat ctatagaatt 4500 atgcagagag gactgttggg caggtcccaa gtaggggtag gagtttttca agaaaacgtg 4560 ttccacacaa tgtggcatgt caccagggga gctgtactca tgtatcaagg gaagagactg 4620 gaaccgagct gggccagtgt caaaaaagac ctgatctcat atggaggagg ttggaggctt 4680 caaggatcct ggaacacggg agaagaagtg caggtgattg ctgttgaacc agggaaaaac 4740 cccaaaaatg tacaaacagc gccgggcacc tttaagaccc ctgaaggtga agttggagcc 4800 attgccctag attttaaacc cggcacatct ggatccccca tcgtgaacag agaaggaaaa 4860 atagtaggtc tttatggaaa tggagtagtg acaacaagtg gaacctacgt cagtgccata 4920 gctcaagcca aagcatcaca agaagggccc ctaccagaga ttgaagacga agtgtttagg 4980 aaaagaaaat taacaattat ggacctacat ccaggatcgg ggaaaacaag aagatatctt 5040 ccagccatag tccgtgaggc cataaaaagg aagctgcgca cactaattct ggctcccaca 5100 agggttgtcg cttccgaaat ggcagaagcg ctcaagggaa tgccaataag gtaccaaaca 5160 acagcagtga agagtgaaca cacaggaaaa gagatagttg acctcatgtg tcacgccact 5220 ttcaccatgc gcctcctgtc tcccgtgaga gttcccaatt acaacatgat tatcatggat 5280 gaagcacatt tcactgatcc atccagcata gcggccagag ggtacatctc aacccgagtg 5340 ggcatgggtg aagcagctgc gattttcatg acagccactc ccccaggatc agtggaggcc 5400 tttccacaga gcaacgcagt tatccaagat gaggaaagag acattcctga gagatcatgg 5460 aactcaggct atgagtggat cactgacttc ccaggtaaaa cagtctggtt tgttccaagc 5520 attaaagcag gaaatgacat tgccaactgc ttaagaaaga atggaaaacg ggtgattcaa 5580 ttgagcagga aaacctttga tacagagtac caaaaaacaa aaaataacga ctgggattat 5640 gtcgtcacaa cagacatctc cgaaatggga gcaaatttcc gggccgacag ggtgatagac 5700 ccaagacggt gtctgaaacc ggtaatacta aaagatggtc cagagcgtgt cattctagca 5760 ggaccgatgc cagtgactgt ggccagtgcc gcccagagga gaggaagaat tggaaggaac 5820 cacaataagg aaggtgacca gtacatctac atgggacagc ctttaaacaa cgatgaagat 5880 cacgctcact ggacagaagc aaaaatgctc cttgataata taaacacacc agaagggatt 5940 atcccagccc tcttcgagcc ggagagagaa aagagtgcag caatagacgg ggaatacaga 6000 ctgcggggtg aagcaaggaa aacgtttgtg gagctcatga gaagaggaga tctacctgtc 6060 tggctatcct acaaagttgc ctcagaaggc ttccagtact ctgacagaag atggtgcttt 6120 gacggggaaa ggaacaacca ggtgttggag gagaacatgg acgtggagat ttggacaaaa 6180 gaaggagaaa gaaagaaact acgaccccgc tggctggatg ccagaacata ctcagaccca 6240 ctggccctgc gcgagtttaa agagtttgca gcaggaagaa gaagcgtctc aggtgatcta 6300 atactagaaa tagggaaact tccacaacac ttgacgcaaa gggcccagaa tgccttggac 6360 aacctggtca tgttgcacaa ctccgaacaa ggaggaagag cctatagaca tgcaatggaa 6420 gaactgccag acaccataga aacgttgatg ctcctagctt tgatagctgt gttaactggt 6480 ggagtgacgc tgttcttcct atcaggaaag ggcctaggga aaacatctat cggcctactc 6540 tgcgtgatgg cttcaagcgt actgctatgg atggccagtg tggagcccca ttggatagcg 6600 gcctccatca tactggagtt cttcctgatg gtgttgctta ttccagagcc agacagacaa 6660 cgcactccgc aggacaacca gctggcatat gtggtgatag gtttgttatt catgatactg 6720 acagtagcag ccaatgagat gggactgctg gaaaccacaa agaaagactt agggattggc 6780 catgtggctg ttgaaaatca ccaccatgcc gcaatgctgg acgtagactt acatccagct 6840 tcagcctgga ccctctatgc agtggccaca acaattatca ctcccatgat gaggcacaca 6900 atcgaaaaca caacggcaaa catttccctg acagccattg caaaccaggc agctatattg 6960 atgggacttg acaaaggatg gccaatatcg aagatggaca taggagttcc acttctcgcc 7020 ttggggtgct attcccaggt gaatccactg acgctgacag cagcggtgtt gatgctagtg 7080 gctcattacr ccataattgg acctggactg caagcaaaag ctactagaga agctcaaaaa 7140 aggacagcgg ccggaataat gaaaaatcca accattgatg gaattgttgc aatagatttg 7200 gaccctgtgg tttatgatgc aaaatttgag aaacaactag gccaaataat gttgttgata 7260 ctatgcacat cacagatcct cttgatgcgg actacatggg ccttgtgcga atccatcaca 7320 ctggccactg gacctctgac cacgctttgg gagggatctc caggaaaatt ttggaacacc 7380 acgatagcgg tttccatggc aaacattttc agaggaagtt atctagcagg agcaggtctg 7440 gccttctcat taatgaaatc tctaggagga ggtaggagag gcacaggagc ccaaggggaa 7500 acactgggag agaaatggaa aagacagctg aaccaactga gcaagtcaga attcaacacc 7560 tataaaagga gtgggattat ggaagtggac agatccgaag ccaaagaggg actgaaaaga 7620 ggagaaacaa ccaaacatgc agtgtcgaga ggaaccgcta aactgaggtg gtttgtggag 7680 aggaaccttg tgaaaccaga agggaaagtc atagacctcg gttgtggaag aggtggctgg 7740 tcatactatt gcgctgggct gaagaaagtc acagaagtga agggatatac aaaaggagga 7800 cctggacatg aggaaccaat cccaatggcg acctatggat ggaacctagt aaaactgcac 7860 tccgggaaag acgtattctt tataccacct gagaaatgtg ataccctttt gtgtgatatt 7920 ggtgagtcct ctccaaaccc aactatagag gaaggaagaa cgctacgcgt tctaaagatg 7980 gtggaaccat ggctcagagg aaaccaattt tgcataaaaa ttctgaatcc ctacatgcca 8040 agtgtggtgg aaactctgga gcaaatgcaa agaaaacatg gaggaatgct agtgcgaaat 8100 ccactttcaa gaaattctac tcatgaaatg tattgggttt catgtggaac aggaaacatt 8160 gtgtcagcag taaacatgac atccagaatg ttgctaaatc gattcacaat ggcccacagg 8220 aaaccaacat atgaaagaga cgtggaccta ggcgccggaa caagacatgt ggcagtggaa 8280 ccagaggtag ccaacctaga tatcattggc cagaggatag agaacataaa acatgaacat 8340 aagtcaacat ggcattatga tgaggacaat ccatataaaa catgggccta tcatggatca 8400 tatgaggtca agccatcagg atcagcctca tccatggtca atggcgtggt gaaactgctc 8460 accaaaccat gggatgtcat ccccatggtc acacaaatag ccatgactga cactacaccc 8520 tttggacaac agagggtgtt taaagagaaa gttgacacac gcacaccaaa agcaaaacga 8580 ggcacagcac aaatcatgga ggtgacagcc aggtggttat ggggttttct ttctagaaac 8640 aagaaaccaa gaatttgtac aagagaggag ttcacaagaa aagttaggtc aaacgcagcc 8700 attggagcag tgttcgttga tgaaaatcaa tggaactcag caaaagaagc agtggaagat 8760 gaccggttct gggaccttgt gcacagagag agggagcttc acaaacaggg aaaatgtgcc 8820 acgtgtgttt acaacatgat ggggaagaga gagaaaaagc taggagagtt cggaaaggca 8880 aaaggaagtc gtgcaatatg gtacatgtgg ttaggagcac gctttctaga gttcgaagct 8940 cttggtttca tgaacgaaga tcactggttc agcagagaga attcactcag cggagtggaa 9000 ggagaaggac ttcacaaact tggatatata ctcagagaca tatcaaagat tccaggggga 9060 aacatgtatg cagatgacac agccggatgg gatacaagga taacagagga tgacctccag 9120 aatgaggcca aaattactga catcatggaa cccgaacatg ccctactggc taagtcaatc 9180 ttcaagctaa cctaccaaaa taaggtggta agggttcaga gaccagcaaa aaatggaacc 9240 gtgatggatg tcatatccag acgtgaccag agaggaagtg gccaggttgg aacttatggc 9300 ttaaacactt tcaccaacat ggaagcccag ctgataagac aaatggagtc tgagggaatc 9360 ttttcaccca acgaattaga gaccccaaat ttagccgaga gagttctcga ctggctggaa 9420 aaatatggcg tcgaaaggct gaaaagaatg gcaatcagtg gagatgactg cgtagtgaaa 9480 ccaattgatg acaggttcgc gacagcctta acagctctga atgatatggg aaaagtraga 9540 aaagatatac cacaatggga accttcaaaa ggatggaatg attggcaaca agtgcctttt 9600 tgttcacacc acttccacca gctgattatg aaggatggga gggaaatagt ggtgccatgc 9660 cgcaaccaag atgaacttgt gggtagggct agagtatcac aaggtgctgg atggagcctg 9720 agagaaactg catgcctagg caagtcatat gcacaaatgt ggcagctgat gtacttccac 9780 aggagagacc tgagactagc cgctaatgct atctgttcag ccgttccagt tgattgggtc 9840 ccaaccagcc gcaccacctg gtcgatccat gcccatcacc aatggatgac aacagaagac 9900 atgctgtcag tatggaatag ggtttggata gaggaaaacc catggatgga ggacaaaacc 9960 cgtatatcca gttgggaaga tgttccatat ctaggaaaaa gggaagatca atggtgtgga 10020 tctctgatag gcttaacagc aagggccacc tgggccacca acatacaagt ggccataaac 10080 caagtgagaa gactaattgg gaatgagaat tattcagatt acatgacatc aatgaagaga 10140 ttcaagaacg agagtgaacc cgaaggggca ctctgg 10176 <210> 30 <211> 10173 <212> DNA <213> Dengue virus type 2 <400> 30 atgaataacc aacggaaaaa ggcgaaaaac acgcctttca atatgctgaa acgcgagaga 60 aaccgcgtgt cgactgtgca acagctgaca aagagattct cacttggaat gctgcaggga 120 cgaggaccat taaaactgtt catggccctg gtagctttcc ttcgtttcct aacaatccca 180 ccaacagcag ggatattgaa gagatgggga acaattaaaa aatcaaaagc cattaatgtt 240 ttgagagggt tcaggaaaga gatcggaagg atgctgaaca tcttgaatag gagacgcagg 300 tctgcaggca tgatcattat gctgattcca acagtgatgg cgttccatct aaccacacgc 360 aacggagaac cacacatgat cgtcggtata caggagaaag ggaaaagtct tctgttcaaa 420 acagagaatg gtgtgaacat gtgcaccctc atggctatgg accttggtga attgtgtgaa 480 gacacaatca cgtacaaatg tcctcttctc aggcagaatg agccagaaga catagactgt 540 tggtgcaact ccacgtccac gtgggtaacc tatgggacct gtaccactac gggagaacat 600 agaagagaaa aaagatcagt ggcactcgtt ccacatgtgg gaatgggact ggagacgcga 660 accgaaacat ggatgtcatc agaaggggct tggaaacatg cccagagaat tgaaacttgg 720 atcctgagac atccaggctt caccataatg gcagcaatcc tggcatacac catagggacg 780 acacatttcc agagagtcct gattttcatt ctactgacag ctgtcgctcc ttcaatgaca 840 atgcgttgca taggaatatc aaatagagac tttgtagaag gggtttcagg aggaagttgg 900 gttgacatag tcttagaaca tggaagctgt gtgacgacga tggcaaaaaa caaaccaaca 960 ttggattttg aactgataaa aacggaagcc aaacagcctg ccaccctaag gaagtactgc 1020 atagaagcaa agctaaccaa cacaacaaca gaatcccgtt gcccaacaca aggggaaccc 1080 agcctaraag aagagcagga taagaggttc gtctgcaaac actccatggt agacagagga 1140 tggggaaatg gatgtgggtt atttggaaag ggaggcattg tgacctgtgc tatgttcaaa 1200 tgcaaaaaga acatggaagg gaaaatcgtg caaccagaaa acttggaata caccattgtg 1260 gtaacacctc actcagggga ggagcatgcg gtcggaaatg acacaggaaa acacggcaag 1320 gaaatcaaag taacaccaca gagttccatc acagaagctg aattgacagg ttatggcacc 1380 gtcacgatgg agtgctcccc gagaacaggc ctcgacttca atgagatggt gttgctgcag 1440 atggaaaata aagcttggct ggtgcatagg caatggtttc tagacctgcc attaccatgg 1500 ctgcccggag cggatraaca agagtcaaat tggatacaga aagaaacatt ggtcactttc 1560 aaaaatcccc atgcgaagaa acaggatgtt gttgttttag gatcccaaga aggggccatg 1620 catacagcac tcacaggagc cacagaaatc caaatgtcgt caggaaactt gctcttcact 1680 ggacatctca agtgcaggct gagaatggac aagctacagc ttaaaggaat gtcatactcc 1740 atgtgcacag gaaagtttaa agttgtgaag gaaatagcag aaacacaaca tggaacgata 1800 gtcatcagag tgcaatatga aggggacggc tctccatgta aaatcccttt tgagataatg 1860 gatttggaaa aaagatatgt cttaggccgc ctgatcacag tcaacccaat tgtaacagaa 1920 aaagatagcc cagtcaacat agaagcagaa cctccattcg gagacagcta catcatcata 1980 ggagtagagc cgggacaact gaagctcaac tggttcaaga aaggaagttc tatcggccaa 2040 atgtttgaga caacgatgag aggggcgaag agaatggcca ttttgggtga cacagcctgg 2100 gacttcggat ccctgggagg agtgtttaca tctataggaa aagctctcca ccaagtcttt 2160 ggagcaatct atggagctgc cttcagtggg gtttcatgga ccatgaaaat cctcatagga 2220 gtcattatca catggatagg aatgaactca cgcagcacct cactgtctgt gtcactagta 2280 ctggtgggaa ttgtgacact gtatttggga gtcatggtgc aggccgatag tggttgcgtc 2340 gtgagctgga aaaacaaaga actgaaatgt ggcagtggga ttttcatcac agacaacgtg 2400 cacacatgga cagaacaata caagttccaa ccagaatccc cttcaaaact agcttcagct 2460 atccagaaag ctcaagaaga gggaatttgt gggatccgct cggtaacaag attggagaac 2520 ctaatgtgga aacaaataac accagaattg aatcacattc tagcagaaaa tgaggtgaag 2580 ttgactatca tgacaggaga catcaaagga atcatgcagg caggaaaacg atctttgcgg 2640 cctcagccca ctgagctaaa gtattcatgg aaaacatggg gtaaagcaaa aatgctctct 2700 acagaatctt ataaccaaac ctttctcatt gatggccccg agacggcaga gtgccccaac 2760 acaaatagag cttggaactc gttggaggtt gaagactacg gctttggagt gttcaccacc 2820 aatatatggc taaaattgaa agaaaaacag gatgcattct gcgactcaaa actcatgtcg 2880 gcggccataa aagacaacag agccgtccat gccgacatgg gttactggat agaaagtgca 2940 ctcaatgaca catggaagat agagaaagcc tctttcattg aagtaaaaaa ctgccactgg 3000 ccaaaatcac acaccctctg gagcaatgga gtgctagaaa gtgagatgat aatcccaaag 3060 aatctcgctg gaccagtgtc tcaacacaac tatagaccag gctaccatac acaagtagca 3120 ggaccatggc acctaggtaa gcttgagatg gactttgatt tctgcgatgg aaccacagta 3180 atagtgactg aggactgcgg aaatagagga ccctctttga gaacaactac tgcctctgga 3240 aaactcataa cggaatggtg ctgccgatct tgcacattac cacccctaag atacagaggt 3300 gagaatgggt gctggtacgg gatggaaatc agaccattga aagagaaaga agaaaatctg 3360 gttaaytcct tggtcacagc cggacatggg caggttgaca atttttcact gggagtcttg 3420 ggaatggcat tgttcctgga ggaaatgctt aggacccgag taggaacgaa acatgcaata 3480 ctactagttg cagtttcttt tgtgacattg atcacaggga acatgtcctt caaagaccta 3540 ggaagagtgg tggttatggt aggcgctacc atggcggatg acataggtat gggcgtgact 3600 tatcttgccc tactagcagc cttcaaagtt agaccaactt ttgcagctgg actactcttg 3660 agaaagctga cctccaagga attgatgatg actaccatag gaattgtact cctctcccaa 3720 agcactatac cagagaccgt tcttgaattg actgacgcgt tagccttagg catgatggtc 3780 cttaaaatag taagaaacat ggaaaagtat caattagcag tgactatcat ggctatcttg 3840 tgcgtcccaa acgcagtgat attgcaaaat gcatggaaag tgagttgcac aatactggca 3900 gtggtgtccg tttccccact gtttttgaca tcctcacagc aaaaaacgga ttggatacca 3960 ttagcattga caatcaaggg tctcaatcca acagccattt ttctaacaac cctctcaaga 4020 accaacaaga aaagaagctg gccactaaat gaggctatca tggcagtcgg aatggtgagc 4080 attttagcca gttccctcct aaaaaatgat attcccatga caggaccact agtggctgga 4140 gggctcctca ctgtgtgcta cgtgctyact ggacgatcgg ccgacttgga actggagaga 4200 gcaaccgatg tcaaatggga agaccaggca gagatatcag gaagcagtcc aatcctgtca 4260 ataacaatat cagaagatgg tagcatgtcg ataaaaaatg aagaggaaga acaaacactg 4320 accatactca ttagaacagg attgctggtg atctcaggac tttttcctat atcaatacca 4380 gtcacggcag cagcatggta cctgtgggaa gtgaagaaac aacgggccgg agtattgtgg 4440 gacgttcctt cacctccacc catgggaaag gctgaactgg aagatggagc ctacagaatc 4500 aagcaaaaag ggattcttgg gtattctcag atcggagctg gagtttacaa agaaggaaca 4560 tttcatacaa tgtggcatgt cacacgtggc gctgttctaa tgcataaagg aaagaggatt 4620 gaaccttcat gggcggacgt caaaaaagat ctaatatcat atggaggagg ctggaagtta 4680 gaaggagaat ggaaggaagg agaagaagtc caggtgctgg cactggagcc tggaaaaaat 4740 ccaagagccg tccaaacaaa acctggtctt ttcaaaacca acaccggaac aataggtgcc 4800 gtatctctgg acttttcccc tggaacgtca ggatctccaa tcatcgacaa aaaaggaaaa 4860 gttgtgggtc tttatggcaa tggtgttgtc acaaggagtg gagcatatgt gagtgctata 4920 gcccaaactg aaaaaagcat tgaagacaac cccgagatca aagatgacat tttccgaaag 4980 agaagactga ccatcatgga cctccaccca ggagcgggaa agacgaaaag ataccttcca 5040 gccatagtca gagaagccat aaaacggggt ttgagaacat taatcttggc ccccactaga 5100 gttgtggcag ctgaaatgga ggaagctctt agaggacttc caataagata ccaaacccca 5160 gccatcagag ctgagcacac cgggcgggaa attgtggatc taatgtgtca tgccacattt 5220 accatgaggc tgctgtcacc agttagagtg ccaaactata acttgattat catggacgaa 5280 gcccatttca cagacccagc aagtatagca gctagaggat acatctcaac ccgagtggag 5340 atgggtgagg cagctgggat tttcatgaca gccactcccc cgggaagtag agatccattt 5400 cctcagagca atgcaccaat catagatgaa gaaagagaaa tccctgaacg ttcatggaat 5460 tctggacatg agtgggtcac ggattttaaa gggaagactg tttggttcgt tccaagcata 5520 aaagcaggaa atgatatagc agcttgcctg agaaaaaatg gaaagaaagt aatacaactt 5580 agtaggaaga cttttgattc tgagtatgtc aagactagaa ccaatgattg ggatttcgtg 5640 gttacaactg acatttcaga aatgggtgcc aatttcaaag ctgagagggt tatagacccc 5700 agacgctgca tgaaaccagt catactaaca gatggtgagg agcgggtgat tctggcagga 5760 cctatgccag tgacccactc tagcgcagca caaagaagag ggagaatagg aagaaatcca 5820 aaaaatgaaa atgaccagta catatacatg ggagaacctt tggaaaatga tgaagactgt 5880 gcacactgga aagaagccaa aatgctccta gataacatta acacaccaga aggaatcatt 5940 cctagtatgt ttgaaccaga gcgtgaaaaa gtggatgcca ttgatggcga ataccgtttg 6000 agaggagaag caaggaaaac ctttgtggac ttaatgagga gaggagactt accagtctgg 6060 ttggcttaca aagtggcagc tgaaggcatc aactatgcag acagaaggtg gtgttttgat 6120 ggaattaaga acaaccaaat cctggaagar aatgtggaag ttgaaatctg gacaaaagaa 6180 ggggaaagga agaaattgaa acccagatgg ttggatgcta ggatctactc tgacccactg 6240 gcgctaaagg aattcaaaga atttgcagcc ggaagaaagt ctctgaccct gaacctaatc 6300 acagaaatgg gtaggcttcc aacattcatg actcagaaga cgagagacgc actggacaac 6360 ctagcagtgc tgcacacggc tgaagcaggt ggaagggcgt acaatcatgc tctcagtgaa 6420 ctgccggaga ccctagagac actgctttta ctgacacttc tggctacagt cacaggaggg 6480 atctttttat tcttgatgag cgggaggggc atagggaaga tgaccctggg aatgtgctgc 6540 ataatcacgg ctagcatcct tctatggtrc gcacaaatac agccacactg gatagcagct 6600 tcaataatac tggagttttt tctcatagtt ttgcttattc cagaaccaga aaaacagaga 6660 acaccccaag acaaccaatt gacctacgtt gtcatagcca tcctcacagt ggtggccgca 6720 accatggcaa acgagatggg tttcctagaa aaaacgaaga aggatcttgg attaggaagc 6780 attgcaaccc agcaacccga gagcaacatc ctggacatag acctacgtcc cgcatcagct 6840 tggacgctgt atgccgtggc tacaacattc gttacaccaa tgctgagaca tagcattgag 6900 aattcctcag tgaatgtatc cctaacagct atagccaacc aagcaacagt gttaatgggt 6960 ctagggaaag gatggccatt gtcaaagatg gacatcggag ttccccttct cgccattgga 7020 tgttactcac aagtcaatcy cataactctt ayagcagctc tcctcttatt ggtagcgcat 7080 tacgccatca ttgggccagg acttcaagca aaagcaacca gagaagccca gaaaagagca 7140 gcggcgggca tcatgaaaaa cccaaccgtc gatggaataa cagtgattga tctagatcca 7200 ataccttatg atccaaagtt tgaaaagcag ttgggacaag tratgctact aatcctctgc 7260 gtgactcaag tattgatgat gaggactaca tgggctctgt gtgaggccyt aaccttagct 7320 acagggccca tctccacact gtgggaagga aatccaggaa ggttctggaa cactaccatt 7380 gcggtgtcaa tggctaacat ctttagaggg agttacctgg ctggagctgg acttctcttt 7440 tccattatga agaacacaac caacacaaga aggggaactg gcaacatagg agagacgctt 7500 ggagagaaat ggaaaagccg attgaacgca ctgggaaaaa gtgaattcca gatctacaag 7560 aaaagtggaa tccaggaagt ggatagaacc ttagcaaaag aaggcatcaa aagaggagaa 7620 acggaccatc acgctgtgtc gcgaggctca gcaaaactga gatggttcgt tgagagaaac 7680 atggtcacac cagaagggaa ggtagtggac ctcggttgtg gcagaggagg ctggtcatac 7740 tattgtggag gactaaaaaa cgtaagagaa gtgaaaggcc taacaaaagg aggaccagga 7800 cacgaagaac ccatccctat gtcaacatat gggtggaatc tggtgcgtct tcagagtgga 7860 gttgacgtct tcttcattcc gccagaaaag tgtgacacat tgttgtgtga cataggggag 7920 tcatcaccaa atcccacagt ggaagcagga cgaacactca gagtccttaa cttagtagaa 7980 aactggttga acaacaacac tcaattttgc ataaaagttc tcaacccata tatgccctca 8040 gttatagaaa aaatggaagc actgcaaagg aaatatggag gagccttagt gagaaatcca 8100 ctctcacgga actccacaca tgagatgtac tgggtatcca atgcttctgg gaacatagtg 8160 tcatcagtga acatgatttc aaggatgttg atcaatagat tcacaatgag acacaagaaa 8220 gccacctacg agccggatgt tgacctcgga agcggaaccc gaaacattgg agttgaaagt 8280 gaaataccaa acctagacat aattgggaaa agaatagaaa aaataaagca agagcatgaa 8340 acatcatggc actacgacca agaccaccca tacaaaacgt gggcatacca tggtagctat 8400 gaaacaaaac agactggatc agcatcatcc atggtcaacg gagtggtcag gctgctgaca 8460 aaaccttggg acgttctccc cayggtgaca cagatggcaa tgacagacac gactccattt 8520 ggacaacagc gtgtttttaa agaaaaagtg gacacgagaa cccaagaacc gaaagaaggc 8580 acgaagaaac taatgagaat cacagcagag tggctgtgga aagaattagg gaagaaaaag 8640 acacctagga tgtgcaccag agaagaattc acaagaaagg kgagaagcaa tgcagccttg 8700 ggggccatat tcactgatga gaacaagtgg aagtcggcac gtgaggctgt tgaagatagt 8760 aggttttggg agctggttga caaggaaagg aatctccatc ttgaaggaaa gtgtgagaca 8820 tgtgtgtaca acatgatggg aaaaagagaa aagaagctag gggaattcgg caaggcaaaa 8880 ggcagcagag ccatatggta catgtggctt ggagctcgct tcctggagtt tgaagcccta 8940 ggattcctaa atgaagatca ctggttctct agagagaact ccctgagtgg agtggaagga 9000 gaagggctgc acaagctagg ttacattcta agagacgtga gcaagaaaga gggaggagcc 9060 atgtacgccg acgacacagc aggatgggac acaagaatca cactagaaga cctaaaaaat 9120 gaagaaatgg taacaaacca catggaagga gaacacaaga aattggccga ggctattttc 9180 aaactaacgt atcaaaacaa ggtggtgcgt gtgcaaagac caacaccaag aggcacagta 9240 atggacatca tatcgagaag agaccaaaga ggtagtgggc aagttggtac atatggactc 9300 aatactttca ccaatatgga agcccaatta atcagacaga tggagggaga aggagtcttt 9360 aaaaacatcc agcacctgac agtcacagaa gaagttgccg tgcaaaactg gttggcaaga 9420 gtggggcgcg aaaggttgtc aagaatggcc atcagtggag atgattgtgt tgtgaaacct 9480 ttagatgaca ggttcgcaaa cgctttgaca gctctaaatg acatgggaaa gattagaaaa 9540 gatatacaac aatgggaacc ttcaaaagga tggaacgatt ggacacaagt gcccttctgt 9600 tcacaccatt tccatgagct aatcatgaag gatggtcgtg tacttgttgt tccatgtaga 9660 aaccaagatg aattgattgg cagagcccga atctcccaag gagcagggtg gtctttgcgg 9720 gagacggcct gtttggggaa gtcttacgcc caaatgtgga gcttgatgta tttccacagg 9780 cgtgacctca ggctggcggc aaatgccatt tgctcggcag tgccatcaca ctgggttcca 9840 acaagtcgaa caacctggtc catacacgcc aaacatgaat ggatgacaac ggaagacatg 9900 ctgacagtct ggaacagggt gtggattcga gaaaacccat ggatggaaga caaaacccca 9960 gtggaatcat gggaggaaat cccatacttg ggaaaaagag aagaccaatg gtgcggctca 10020 ttgattgggt taacaagcag ggccacatgg gcaaagaaca tccaagcagc aataaatcag 10080 gttagatctc ttataggcaa tgaggaatac acagattaca tgccatccat gaaaagattt 10140 agaagagaag aggaagaagc aggagtcctg tgg 10173 <210> 31 <211> 10170 <212> DNA <213> Dengue virus type 3 <400> 31 atgaacaacc aacggaaaaa gacgggaaaa ccgtctatca atatgctgaa acgcgtgaga 60 aaccgtgtgt caactggatc acagttggcg aagagattct caagaggatt gctgaacggc 120 caaggaccaa tgaaattggt tatggcattt atagctttcc tcagatttct agccattcca 180 ccgacagcag gagtcttggc tagatggggt acctttaaga agtcgggggc tattaaggtc 240 ttaaaaggct tcaagaagga gatctcaaac atgctgagca ttatcaacaa acggaaaaag 300 acatcgctct gtctcatgat gatgttacca gcaacacttg ctttccactt aacttcacga 360 gatggagagc cgcgcatgat tgtggggaag aatgaaagag gaaaatccct acttttcaag 420 acagcctctg gaatcaacat gtgcacactc atagctatgg atctgggaga gatgtgtgat 480 gacacggtca cttacaaatg cccccacatt accgaagtgg agcctgaaga cattgactgc 540 tggtgcaacc ttacatcgac atgggtgact tatggaacat gcaatcaagc tggagagcat 600 agacgcgata agagatcagt ggcgttagct ccccatgttg gcatgggact ggacacacgc 660 actcaaacct ggatgtcggc tgaaggagct tggagacaag tcgagaaggt agagacatgg 720 gcccttaggc acccagggtt taccatacta gccctatttc ttgcccatta cataggcact 780 tccttgaccc agaaagtggt tatttttata ctattaatgc tggttacccc atccatgaca 840 atgagatgtg taggagtagg aaacagagat tttgtggaag gcctatcggg agctacgtgg 900 gttgacgtgg tgctcgagca cggtgggtgt gtgactacca tggctaagaa caagcccacg 960 ctggacatag agcttcagaa gaccgaggcc acccaactgg cgaccctaag gaagctatgc 1020 attgagggaa aaattaccaa cataacaacc gactcaagat gtcccaccca aggggaagcg 1080 attttacctg aggagcagga ccagaactac gtgtgtaagc atacatacgt ggacagaggc 1140 tggggaaacg gttgtggttt gtttggcaag ggaagcttgg tgacatgcgc gaaatttcaa 1200 tgtttagaat caatagaggg aaaagtggtg caacatgaga acctcaaata caccgtcatc 1260 atcacagtgc acacaggaga ccaacaccag gtgggaaatg aaacgcaggg agtcacggct 1320 gagataacac cccaggcatc aaccgctgaa gccattttac ctgaatatgg aaccctcggg 1380 ctagaatgct caccacggac aggtttggat ttcaatgaaa tgatctcatt gacaatgaag 1440 aacaaagcat ggatggtaca tagacaatgg ttctttgact tacccctacc atggacatca 1500 ggagctacag cagaaacacc aacttggaac aggaaagagc ttcttgtgac atttaaaaat 1560 gcacatgcaa aaaagcaaga agtagttgtt cttggatcac aagagggagc aatgcataca 1620 gcactgacag gagctacaga gatccaaacc tcaggaggca caagtatctt tgcggggcac 1680 ttaaaatgta gactcaagat ggacaaattg gaactcaaag ggatgagcta tgcaatgtgc 1740 ttgggtagct ttgtgttgaa gaaagaagtc tccgaaacgc agcatgggac aatactcatt 1800 aaggttgagt acaaagggga agatgcaccc tgcaagattc ctttctccac ggaggatgga 1860 caaggaaaag ctcacaatgg cagactgatc acagccaatc cagtggtgac caagaaggag 1920 gagcctgtca acattgaggc tgaacctcct tttggagaaa gtaacatagt aattggaatt 1980 ggagacaaag ccctgaaaat caactggtac aagaagggaa gctcgattgg gaagatgttc 2040 gaggctactg ccagaggtgt aaggcgcatg gccatcttgg gagacacagc ctgggacttt 2100 ggatcagtgg gtggtgtttt gaattcatta gggaaaatgg tccaccaaat atttgggagt 2160 gcttacacag ccctatttgg tggagtctcc tggatgatga aaattggaat aggtgtcctc 2220 ttaacctgga tagggttgaa ctcaaaaaat acttctatgt cattttcatg catcgcgata 2280 ggaatcatta yactctatct gggagccgtg gtgcaagctg acatggggtg tgtcataaac 2340 tggaaaggca aagaactcaa atgtggaagt ggaattttcg tcactaatga ggtccacacc 2400 tggacagagc aatacaaatt tcaagcagac tcccccaaga gactggcaac agccattgca 2460 ggcgcttggg aaaatggagt gtgcggaatt aggtcaacaa ccagaatgga gaacctcttg 2520 tggaagcaaa tagccaatga actgaattac atattatggg aaaacaacat taaattaacg 2580 gtagttgtag gcgacataac tggggtctta gagcaaggga aaagaacact aacaccacaa 2640 cccatggagc taaaatattc ttggaaaaca tggggaaagg caaaaatagt gacagctgaa 2700 acacaaaatt cctctttcat aatagatggg ccaagcacac cggagtgtcc aagtgcctca 2760 agagcatgga atgtgtggga ggtggaggat tacgggttcg gagttttcac aaccaacata 2820 tggctgaaac tccgagaggt gtacacccaa ctatgtgacc ataggctaat gtcggcagcc 2880 gtcaaggatg agagggctgt acatgccgac atgggctatt ggatagaaag ccaaaagaat 2940 gggagttgga agctagaaaa agcatccttc atagaggtga aaacctgcac atggccaaaa 3000 tcacacactc tctggagcaa tggtgtgcta gagagtgaca tgattatccc aaagagtcta 3060 gctggtccca tttcgcaaca caaccacagg cccgggtacc acacccaaac ggcaggaccc 3120 tggcacttag gaaaattgga gctggacttc aactattgtg aaggaacaac agttgtcatc 3180 tcagaaaact gtgggacaag aggcccatca ttgagaacaa caacggtgtc agggaagttg 3240 atacacgaat ggtgctgccg ctcgtgcaca cttcctcccc tacgatacat gggagaagac 3300 ggctgctggt atggcatgga aatcagaccc attaatgaga aagaagagaa tatggtaaag 3360 tctctagcct cagcagggag tggaaaggtg gacaacttca caatgggtgt cttgtgtttg 3420 gcaatcctct ttgaagaggt gatgagagga aaatttggga aaaaacacat gattgcaggg 3480 gttctcttca cgtttgtgct cctcctctca gggcaaataa catggagaga catggcgcac 3540 acactcataa tgattgggtc caacgcctct gacagaatgg ggatgggcgt cacttaccta 3600 gctctaattg caacatttaa aattcagcca ctcctggctt tgggattctt cctgaggaaa 3660 ctgacatcta gagaaaattt attgctggga gttgggttgg ccatggcaac aacgttacga 3720 ctgccagagg acattgaaca gatggcgaat ggaattgctt tggggctcat ggctcttaaa 3780 ctgataacac aatttgaaac ataccaacta tggacggcat tagtttccct aacgtgttca 3840 aatacaattt tcacgttgac tgttgcctgg agaacagcca ctctgatttt agccggaatt 3900 tcgcttttgc cagtgtgcca gtcttcgagc atgaggaaaa cagattggct cccaatgact 3960 gtggcagcta tgggagctca acccctacca ctttttattt tcagtctgaa agatacactc 4020 aaaaggagaa gctggccact gaatgagggg gtgatggcag ttggacttgt gagcattcta 4080 gctagttctc tccttaggaa tgatgtgcct atggctggac cattagtggc tgggggcttg 4140 ctgatagcgt gctacgtcat aactggcacg tcagcagacc tcactgtaga aaaggcagca 4200 gatgtaacat gggaggaaga ggccgagcaa acaggagtgt cccacaattt aatggtcaca 4260 gttgatgatg atggaacaat gagaataaaa gatgacgaga ctgagaacat cttaacagtg 4320 cttttaaaaa cagcactact aatagtatca ggcatctttc catactccat acccgcaaca 4380 ctgttggtct ggcatacttg gcaaaagcaa acccaaagat ccggcgtcct atgggacgta 4440 cccagccccc cagagacaca gaaagcggaa ctggaagaag gggtctatag gatcaaacag 4500 caaggaattt ttgggaaaac ccaagtgggg gttggagtac agaaagaagg agttttccac 4560 accatgtggc atgtcacaag aggggcagtg ttgacacaca atgggaaaag actggaacca 4620 aactgggcta gcgtgaaaaa agatctgatt tcatacggag gaggatggag attgagtgca 4680 caatggaaaa agggggagga ggtgcaggtt attgccgtag agcctgggaa gaacccaaag 4740 aactttcaaa ccatgccagg catttttcag acaacaacag gggaaatagg agcaattgca 4800 ctggatttca agcctggaac ttcaggatct cccatcataa acagagaggg aaaggtagtg 4860 ggactgtatg gcaatggagt ggttacaaag aatggaggct atgtcagtgg aatagcgcaa 4920 acaaatgcag aaccagatgg accgacacca gagttggaag aagagatgtt caaaaagcga 4980 aatctaacca taatggatct ccatcctggg tcaggaaaga cgcggaaata tcttccagct 5040 attgttagag aggcaatcaa gagacgctta aggactctaa ttttggcacc aacaagggta 5100 gttgcagctg agatggaaga agcattgaaa gggctcccaa taaggtatca aacaactgca 5160 acaaaatctg aacacacagg aagagagatt gttgatctaa tgtgtcacgc aacgttcaca 5220 atgcgcttgc tgtcaccagt cagggttcca aactacaact tgataataat ggatgaggcc 5280 catttcacag acccagccag tatagcggct agagggtaca tatcaactcg tgtaggaatg 5340 ggagaggcag ccgcaatttt catgacagca acaccccctg gaacagctga tgcctttcct 5400 cagagcaacg ctccaattca agatgaagag agagacatac cggaacgctc atggaattca 5460 ggcaatgaat ggattactga ctttgttggg aagacagtgt ggtttgtccc tagcatcaaa 5520 gccggaaatg acatagcaaa ctgcttgcgg aaaaatggaa aaaaggttat tcaactcagc 5580 aggaagacct ttgacacaga atatcaaaag accaaactga atgattggga ctttgtggtg 5640 acaacagaca tttcagaaat gggagccaat ttcaaagcag atagagtgat cgacccaaga 5700 agatgtctca agccggtgat tttgacagat ggacccgagc gggtgatcct ggctggacca 5760 atgccagtca ccgtagcgag cgctgcgcaa aggagaggga gagttggcag gaacccacaa 5820 aaagaaaatg accagtacat attcatgggc cagcctctca acaatgatga agaccatgct 5880 cactggacag aagcaaaaat gctgctggac aacatcaaca caccagaagg gattatacca 5940 gctctctttg aaccagaaag ggagaagtca gccgccatag acggcgaata ccgcctgaag 6000 ggtgagtcca ggaagacttt cgtggaactc atgaggaggg gtgacctccc agtttggcta 6060 gcccataaag tagcatcaga agggatcaaa tatacagata gaaaatggtg ctttgatgga 6120 gaacgtaata atcaaatttt agaggagaat atggatgtgg aaatctggac aaaggaagga 6180 gaaaagaaaa aactgagacc taggtggctt gatgcccgca cttattcaga tcctttagca 6240 ctcaaagaat tcaaggattt tgcagctggc agaaagtcaa tcgcccttga tcttgtgaca 6300 gaaataggaa gagtgccttc acacttagcc cacagaacga gaaacgccct ggataatttg 6360 gtgatgctgc acacgtcaga acatggcggt agggcctaca ggcatgcagt ggaggaacta 6420 ccagaaacga tggaaacact cttactcctg ggactgatga tcttgttaac aggtggagca 6480 atgctcttct tgatatcagg taaagggatt ggaaagactt caataggact catttgtgta 6540 attgcttcca gcggcatgtt atggatggct gatgtcccac tccaatggat cgcatcggct 6600 atagtcctgg agttttttat gatggtgttg ctcataccag aaccagaaaa gcagagaact 6660 ccccaagaca accaactcgc atatgtcgtg ataggcatac ttacattggc tgcaatagta 6720 gcggccaatg aaatgggact gttggaaact acaaagagag atttaggaat gtctaaagaa 6780 ccaggtgttg tttctccaac cagctatttg gatgtggact tgcacccagc atcagcctgg 6840 acattgtacg ccgtggccac aacagtaata acaccaatgt tgagacacac catagagaat 6900 tccacagcaa atgtgtctct ggcagccata gctaaccagg cagtggtcct gatgggttta 6960 gacaaaggat ggccgatatc gaaaatggac ttgggcgtac cactattggc actgggttgc 7020 tattcacaag tgaacccact aactcttgca gcggcagtac ttttgctagt cacacattat 7080 gcaattatag gtccaggatt gcaggcaaaa gccacccgtg aagctcagaa aaggacagct 7140 gctggaataa tgaagaatcc aacggtggat ggaataatga caatagacct agatcctgta 7200 atatatgatt caaaatttga aaagcaacta ggacaggtca tgctcctggt tctgtgtgca 7260 gtccaacttt tattgatgag aacatcatgg gccttgtgtg aagttytaac cctagccaca 7320 ggaccaataa caacactctg ggaaggatca cctgggaagt tctggaacac cacgatagct 7380 gtttccatgg cgaacatctt tagagggagc tatttagcag gagctgggct tgctttttct 7440 atcatgaaat cagttggaac aggaaagaga ggaacagggt cacaaggtga aaccttagga 7500 gaaaagtgga aaaagaaatt aaatcagtta tcccggaaag agtttgacct ttacaagaaa 7560 tccggaatca ccgaagtgga tagaacagaa gccaaagaag ggttaaaaag aggagaaata 7620 acacaccatg ccgtgtccag aggcagcgca aaacttcaat ggttcgtgga gagaaacatg 7680 gtcattcctg aaggaagagt catagaccta ggctgtggaa gaggaggctg gtcatattac 7740 tgtgcaggac tgaaaaaagt tacagaagtg cgaggataca caaaaggcgg cccaggacac 7800 gaagaaccag tacctatgtc tacatacgga tggaacatag tcaagttaat gagtggaaag 7860 gatgtttttt atctgccacc tgaaaagtgt gataccctat tgtgtgacat tggagaatct 7920 tcaccaagcc caacagtgga agaaagcaga accataagag ttttgaagat ggttgaacca 7980 tggctaaaga acaaccagtt ttgcattaaa gtattgaacc catacatgcc aactgtgatt 8040 gagcacttag aaagactaca aaggaaacat ggaggaatgc ttgtgagaaa tccactctca 8100 cgaaactcca cgcacgaaat gtattggata tccaatggta caggcaatat cgtctcttca 8160 gtcaacatgg tatccagatt gctactgaac agattcacaa tgacacacag gagacccacc 8220 atagagaaag atgtggatct aggagcagga acccgacatg tcaatgcgga accagaaaca 8280 cccaacatgg atgtcattgg ggaaagaata aaaaggatca aagaggagca tagttcaaca 8340 tggcactatg atgatgaaaa tccttacaaa acgtgggctt accatggatc ctatgaagta 8400 aaagccacag gctcagcctc ctccatgata aatggagtcg tgaaactcct cacaaaacca 8460 tgggatgtgg tgcccatggt gacacagatg gcaatgacag atacaactcc attcggccag 8520 caaagagttt ttaaagagaa agtggacacc aggacaccta ggcccatgcc aggaacaaga 8580 aaggttatgg agatcacagc ggagtggctt tggaggaccc tgggaaggaa caaaagaccc 8640 agattatgca caagggagga attcacaaag aaggtcagaa ccaacgcagc tatgggcgct 8700 gtcttcacag aagagaacca atgggacagt gcgagagctg ctgttgagga cgaagaattt 8760 tggaaacttg tggacagaga acgtgaactc cacaaactgg gcaagtgtgg aagctgcgtt 8820 tacaacatga tgggcaagag agagaaaaaa cttggagagt ttggtaaagc aaaaggcagt 8880 agggctatat ggtacatgtg gttgggagcc aggtaccttg agttcgaggc gctcggattc 8940 ctcaatgaag accactggtt ctcgcgtgaa aactcttaca gtggagtaga aggagaagga 9000 ctgcacaagc tgggatacat cttgagagat atttccaaga tacccggagg agccatgtat 9060 gctgatgaca cagccggttg ggacacaaga ataacagaag atgacctgca caatgaggaa 9120 aaaatcacac agcagatgga ccctgaacac aggcagctag cgaacgctat attcaagctc 9180 acataccaaa acaaagtggt caaagtccaa cgaccaactc caaagggcac ggtaatggac 9240 atcatatcta ggaaagacca aagaggcagt ggacaggtgg gaacttatgg tctgaacaca 9300 ttcaccaaca tggaagccca gctaatcaga caaatggaag gagaaggcgt gttgtcaaag 9360 gcagacctcg agaaccccca tccgctagag aagaaaatta cacaatggtt ggaaactaaa 9420 ggagtggaaa ggttaaaaag aatggccatc agcggggatg attgcgttgt gaaaccaatc 9480 gacgacagat tcgccaatgc cctgcttgcc ctgaacgata tgggaaaggt tagaaaggac 9540 atacctcaat ggcagccatc aaagggatgg catgattggc aacaggtccc cttctgctcc 9600 caccactttc atgaattgat catgaaagat ggaagaaagt tggtagttcc ctgcagaccc 9660 caggacgaac taataggaag agcgagaatc tcccaaggag caggatggag ccttagagaa 9720 actgcatgtc tagggaaagc ctacgctcaa atgtgggctc tcatgtattt tcacagaaga 9780 gatcttagac tagcatccaa cgccatatgt tcagcagtac cagtccactg ggtccccacg 9840 agcagaacga catggtctat tcatgctcac catcagtgga tgactacaga agacatgctt 9900 actgtctgga acagggtgtg gatagaggac aatccatgga tggaagacaa aactccagtc 9960 acaacgtggg aagatgttcc atatctaggg aagagagaag accaatggtg cggatcactc 10020 ataggtctca cttccagagc aacctgggcc cagaacatac tcacagcaat ccaacaggtg 10080 agaagcctca taggcaatga agagtttctg gactacatgc cttcgatgaa gagattcagg 10140 aaggaggagg agtcagaggg agccatttgg 10170 <210> 32 <211> 10161 <212> DNA <213> Dengue virus type 4 <400> 32 atgaaccaac gaaaaaaggt ggttagacca cctttcaata tgctgaaacg cgagagaaac 60 cgcgtatcaa cccctcaagg gttggtgaag agattctcaa ccggactttt ttctgggaaa 120 ggacccttac ggatggtgct agcattcatc acgtttttgc gagtcctttc catcccacca 180 acagcaggga ttctgaaaag atggggacag ttgaagaaaa ataaggccat caggatactg 240 attggattca ggaaggagat aggccgcatg ctgaacatct tgaacgggag aaaaaggtca 300 acgataacat tgctgtgctt gattcccacc gtaatggcgt ttcacttgtc aacaagagat 360 ggcgaacccc tcatgatagt ggcaaaacat gaaaggggga gacctctctt gtttaagaca 420 acagagggga tcaacaaatg cactctcatt gccatggact tgggtgaaat gtgtgaggac 480 actgtcacgt ataaatgccc cttactggtc aataccgaac ctgaagacat tgattgctgg 540 tgcaatctca cgtctacctg ggtcatgtat gggacatgca cccagagcgg agaacggaga 600 cgagagaagc gctcagtagc tttaacacca cattcaggaa tgggattgga aacaagagct 660 gagacrtgga tgtcatcgga aggggcttgg aagcatgctc agagagtaga gagctggata 720 ctcagaaacc caggattcgc gctcttggca ggatttatgg cttatatgat tgggcaaaca 780 ggaatccagc gaactgtctt ctttgtccta atgatgctgg tcgccccatc ctacggaatg 840 cgatgcgtag gagtaggaaa cagagacttt gtggaaggag tctcaggtgg agcatgggtc 900 gatctggtgc tagaacatgg aggatgcgtc acaaccatgg cccagggaaa accaaccttg 960 gattttgaac tgactaagac aacagccaag gaagtggctc tgttaagaac ctattgcatt 1020 gaagcctcaa tatcaaacat aaccacggca acaagatgtc caacgcaagg agagccttat 1080 ctaaaagagg aacaagacca acagtacatt tgccggagag atgtggtaga cagagggtgg 1140 ggcaatggct gtggcttgtt tggaaaagga ggagttgtga catgtgcgaa gttttcatgt 1200 tcggggaaga taacaggcaa tttggtccaa attgagaacc ttgaatacac agtggttgta 1260 acagtccaca atggagacac ccatgcagta ggaaatgaca catccaatca tggagttaca 1320 gccacgataa ctcccaggtc accatcggtg gaagtcaaat tgccggacta tggagaacta 1380 acactcgatt gtgaacccag gtctggaatt gactttaatg agatgattct gatgaaaatg 1440 aaaaagaaaa catggcttgt gcataagcaa tggtttttgg atctacctct accatggaca 1500 gcaggagcag acacatcaga ggttcactgg aattacaaag agagaatggt gacatttaag 1560 sttcctcatg ccaagagaca ggatgtgaca gtgctgggat ctcaggaagg agccatgcat 1620 tctgccctcg ctggagccac agaagtggac tccggtgatg gaaatcacat gtttgcagga 1680 catctcaagt gcaaagtccg tatggagaaa ttgagaatca agggaatgtc atacacgatg 1740 tgttcaggaa agttctcaat tgacaaagag atggcagaaa cacagcatgg gacaacagtg 1800 gtgaaagtca agtatgaagg tgctggagct ccgtgtaaag tccccataga gataagagat 1860 gtgaacaagg aaaaagtggt tgggcgtatc atctcatcca cccctttggc tgagaatacc 1920 aacagtgcaa ccaacataga gttagaaccc ccctttgggg acagctacat agtgataggt 1980 gttggaaaca gtgcattaac actccattgg ttcaggaaag ggagttccat tggcaagatg 2040 tttgagtcca catacagagg tgcaaaacga atggccattc taggtgaaac agcttgggat 2100 tttggttccg ttggtggact gttcacatca ttgggaaagg ctgtgcacca ggtttttgga 2160 agtgtgtata caaccatgtt tggaggagtc tcatggatga ttagaatcct aattgggttc 2220 ctagtgttgt ggattggcac gaactcaagg aacacttcaa tggctatgac gtgcatagct 2280 gttggaggwa tcactctgtt tctgggcttc acagttcaag cagacatggg ttgtgtggtg 2340 tcatggagtg ggaaagaatt gaagtgtgga agcggaattt ttgtggttga caacgtgcac 2400 acttggacag aacagtacaa atttcaaccg gagtccccag cgagactagc gtctgcaata 2460 ttgaatgccc acaaagatgg ggtctgtgga attagatcaa ccacgaggct ggaaaatgtc 2520 atgtggaagc aaataaccaa cgagctaaat tatgttctct gggaaggagg acatgacctc 2580 actgtagtgg ctggggatgt gaagggggtg ttgaccaaag gcaagagagc actcacaccc 2640 ccagtgaatg atctgaaata ttcatggaag acatggggaa aagcaaaaat cttcacccca 2700 gaagcaagaa atagcacatt tttaatagac ggaccagaca cctccgaatg ccccaatgaa 2760 cgaagagcat ggaactttct tgaggtggaa gactatggat ttggcatgtt cacgaccaac 2820 atatggatga aattccgaga aggaagttca gaagtgtgtg accacaggtt aatgtcagcg 2880 gcaattaaag atcagaaagc tgtgcatgct gacatgggtt attggataga gagctcaaaa 2940 aaccagacct ggcagataga gaaagcatct cttattgaag tgaaaacatg tctgtggccc 3000 aagacccaca cattgtggag caatggagtg ctggaaagcc agatgctcat tccaaaatca 3060 tatgcgggcc ctttttcaca ccacaattac cgccagggct atgccacgca aaccgtgggc 3120 ccatggcact taggcaaatt agagatagac tttggagaat gccccggaac aacagtcgca 3180 attcaggagg attgtgacca tagaggccca tctttgagga ccaccactgc atctggaaaa 3240 ctagtcacgc aatggtgctg ccgctcctgc acgatgcctc ccttaaggtt cttgggagaa 3300 gatgggtgct ggtatgggat ggagattagg cccttgagtg aaaaagaaga gaacatggtc 3360 aaatcacagg taacggccgg acagggcaca tcagaaactt tttctatggg tctgttgtgc 3420 ctgaccttgt ttgtggaaga atgcttgagg agaagagtca ctaggaaaca catgatattg 3480 gttgtggtga tcactctttg tgccatcatc ctaggaggcc tcacatggat ggacttacta 3540 cgagccctca tcatgttggg ggacactatg tctggtagaa taggaggaca gatccaccta 3600 gccatcatgg cagtgttcaa gatgtcacca ggataygtgc tgggtgtgtt tttaaggaaa 3660 ctcacttcaa gagagacagc actaatggta ataggaatgg ccatgacaac ggtgctttca 3720 attccacatg accttatgga actcattgat ggaatatcac tggggctaat tttgctaaaa 3780 atagtgacac attttgacaa cacccaagtg ggaaccttag ccctttcctt gaccttcata 3840 agatcaacaa tgccattggt catggcttgg aggaccatta tggctgtgtt gtttgtggtc 3900 acactcattc ctttgtgcag gacaagctgt cttcaaaaac agtctcattg ggtagaaata 3960 acagcactca tcctaggagc ccaagctctg ccagtgtacc taatgactct tatgaaagga 4020 gcctcaagaa gatcttggcc tcttaacgag ggcataatgg ctgtgggttt ggttagtctc 4080 ttaggaagcg ctcttttaaa gaatgatgtc cctttrgctg gcccaatggt ggcaggaggc 4140 ttacttctgg cggcttacgt gatgagtggt agctcagcag atctgtyact agagaaggcc 4200 gccaatgtgc agtgggatga aatggcagac ataacaggct caagcccaat catagaagtg 4260 aagcaggatg aagatggctc tttctccata cgggacgtcg aggaaaccaa tatgataacc 4320 cttttggtga aactggcact gataacagtg tcaggtctct accccttggc aattccagtc 4380 acaatgacct tatggtacat gtggcaagtg aaaacacaaa gatcaggagc cctgtgggac 4440 gtcccctcac ccgctgccac tcaaaaagcc gcactgtctg aaggagtgta caggatcatg 4500 caaagagggt tatttkggaa aactcaggtt ggagtaggga tacacatgga aggtgtattt 4560 cacacaatgt ggcatgtaac aagaggatca gtgatctgcc atgagactgg gagattggag 4620 ccatcttggg ctgacgtcag gaatgacatg atatcatacg gtgggggatg gagacttgga 4680 gacaaatggg acaaagaaga agatgttcag gtcctcgcca tagaaccagg aaaaaatcct 4740 aaacatgtcc aaacgaaacc cggccttttc aagaccctaa ctggagaaat tggagcagta 4800 acattagatt tcaaacccgg aacgtctggt tctcccatca tcaacaggaa aggaaaagtc 4860 atcggactct atggaaatgg agtagttacc aaatcaggtg attacgtcag tgccataacg 4920 caagccgaaa gaattggaga gccagattat gaagtggatg aggacatttt tcgaaagaaa 4980 agattaacta taatggactt acaccccgga gctggaaaga caaaaagaat tcttccatca 5040 atagtgagag aagccttaaa aaggaggctg cgaaccttga ttttggctcc cacgagagtg 5100 gtggcggccg agatggaaga ggccctacgt ggactgccaa tccgttatca gaccccagct 5160 gtgaaatcag aacacacagg aagagagatt gtagacctca tgtgtcatgc aaccttcaca 5220 acaagacttt tgtcatcaac cagagttcca aattacaacc tcatagtgat ggatgaagca 5280 catttcaccg atccttctag tgtcgcggct agaggataca tctcgaccag ggtggaaatg 5340 ggagaggcag cagccatctt catgaccgca acccctcccg gagcgacaga tccctttccc 5400 cagagcaaca gcccaataga agacatcgag agggaaattc cggaaaggtc atggaacaca 5460 gggttcgact ggataacaga ctaccaaggg aaaactgtgt ggtttgttcc cagcataaaa 5520 gctggaaatg acattgcaaa ttgtttgaga aagtcgggaa agaaagttat ccagttgagt 5580 aggaaaacct ttgatacaga gtatccaaaa acgaaactca cggactggga ttttgtggtc 5640 actacagaca tatctgaaat gggggccaat tttagagctg ggagagtgat agaccctagg 5700 agatgcctca agccagttat cctaacagat gggccagaga gagtcatttt agcaggtcct 5760 attccagtga ctccagcaag cgctgctcag agaagagggc gaataggaag gaacccagca 5820 caagaagacg accaatacgt tttctccgga gacccactaa aaaatgatga agatcatgcc 5880 cactggacag aagcaaagat gctgcttgac aatatctaca ccccagaagg gatcattcca 5940 acattgtttg gtccggaaag ggaaaaaacc caagccattg atggagagtt tcgcctcaga 6000 ggggaacaaa ggaagacttt tgtggaatta atgaggagag gagaccttcc ggtgtggctg 6060 agctataagg tagcttctgc tggcatttct tacaaagatc gggaatggtg cttcacaggg 6120 gaaaggaata accaaatttt agaagaaaac atggaggttg aaatttggac tagagaggga 6180 gaaaagaaaa agctaaggcc aagatggtta gatgcacgtg tatacgctga ccccatggct 6240 ttgaaggatt tyaaggagtt tgctagtgga aggaagagca taactctcga catcctaaca 6300 gagattgcca gtttgccaac ttacctttcc tctagggcca agctcgccct tgataacata 6360 gtcatgctcc acacaacaga aagaggaggg agggcctacc aacacgccct gaacgaactc 6420 ccggagtcac tggaaacact tatgcttgta gctttactag gtgctatgac agcaggtatc 6480 ttcctgtttt tcatgcaagg gaaaggaata gggaaattgt caatgggttt gataaccatt 6540 gcggtggcta gtggcttgtt ctgggtagca gaaattcaac cccagtggat agcggcctca 6600 atcatactag agttttttct catggtactg ttgataccgg aaccagaaaa acaaaggacc 6660 ccacaagaca atcaattgat ctacgtcata ttgaccattc tcaccattat tggtctcata 6720 gcagccaacg agatggggct gattgaaaaa acaaaaacgg attttgggtt ttaccaggta 6780 aaaacagaaa ccaccatcct cgatgtggac ttgagaccag cttcagcatg gacgctctat 6840 gcagtagcca ccacaattct gactcccatg ctgagacaca ccatagaaaa cacgtcggcc 6900 aacctatctc tagcagccat tgccaaccag gcggccgtcc taatggggct tggaaaagga 6960 tggccgctcc acagaatgga cctcggtgtg ccgctgttag caatgggatg ctattctcaa 7020 gtgaacccaa caactttgac agcatcctta gtcatgcttt cagtccatta tgcaataata 7080 ggtccaggat tgcaggcaaa agccacaaga gaggcccaga aaaggacagc tgctgggatc 7140 atgaaaaacc ccacggtgga cgggataaca gtaatagatc tagaaccaat atcctatgac 7200 ccaaaatttg aaaagcaatt agggcaggtc atgctactcg tcttgtgtgc tggacaacta 7260 ctcttgatga gaacaacatg ggctttctgt gaagtcttga ctttggccac aggaccaatc 7320 ttgaccttgt gggagggcaa cccgggaagg ttttggaaca cgaccatagc cgtatccacc 7380 gccaacattt tcaggggaag ttacctggcg ggagctggac tggctttttc actcataaag 7440 aatgtacaaa cccctaggag gggaactggg accacaggag agacactggg agagaagtgg 7500 aagagacagc taaactcatt agacagaaaa gagtttgaag agtataaaag aagtggaata 7560 ctagaagtgg acaggactga agccaagtct gccctgaaag atgggtctaa aatcaagcat 7620 gcagtatcta gagggtccag taagattaga tggattgttg agagagggat ggtaaagcca 7680 aaagggaaag ttgtagatct tggctgtggg agaggaggat ggtcttatta catggcgacg 7740 ctcaagaacg tgactgaagt gaaagggtat acaaaaggag gtccaggaca tgaagaaccg 7800 attcccatgg ctacttatgg ctggaatttg gtcaaactcc attcaggggt tgacgtgttc 7860 tacaaaccca cagagcaagt ggacacyctg ctctgtgata ttggggagtc atcttctaat 7920 ccaacaatag aggaaggaag aacattaaga gttttgaaga tggtggagcc atggctctct 7980 tcaaaacctg aattctgcat caaagtcctt aacccctaca tgccaacagt catagaagag 8040 ctggagaaac tgcagagaaa acatggtggg aaccttgtca gatgcccgct gtccaggaac 8100 tccacccatg agatgtattg ggtgtcagga gcgtcgggaa acattgtgag ctctgtgaac 8160 acaacatcaa agatgttgtt gaacaggttc acaacaaggc ataggaaacc cacttatgag 8220 aaggacgtag atcttggggc aggaacgaga agtgtctcca ctgaaacaga aaaaccagac 8280 atgacaatta ttgggagaag gcttcagcga ttgcaagagg agcacaaaga aacctggcat 8340 tatgatcagg aaaacccata cagaacctgg gcgtatcatg gaagctatga agctccttcg 8400 acaggctctg catcctccat ggtgaacggg gtagtaaaac tgctaacaaa accttgggat 8460 gtggttccaa tggtgaccca gttagccatg acagacacaa ccccttttgg gcaacaaaga 8520 gtgttcaaag agaaggtgga taccagaaca ccacaaccaa aacccggtac acgaatggtt 8580 atgaccacga cagccaattg gctgtgggcc ctccttggga agaagaaaaa tcccagactg 8640 tgcacaaggg aagagttcat ctcaaaagtt agatcaaacg cagccatagg cgcagtcttt 8700 caggaagaac agggatggac atcagccagt gaagctgtga atgacagccg gttttgggaa 8760 ctggttgaca aagaaagggc cctacaccag gaagggaaat gtgaatcgtg tgtctacaac 8820 atgatgggaa aacgtgagaa aaagttagga gagtttggca gagccaaggg aagccgagca 8880 atctggtaca tgtggctggg agcgcggttt ctggaatttg aagccctggg ttttttgaat 8940 gaagatcact ggtttggcag agaaaattca tggagtggag tggaagggga aggtctgcac 9000 agattgggat atatcctgga ggagatagac aagaaggatg gagacctaat gtatgctgat 9060 gacacagcag gctgggacac aagaatcact gaggatgacc ttcaaaatga agaactgatc 9120 acggaacaga tggcccccca ccacaagatc ctagccaaag ccattttcaa actaacctat 9180 caaaacaaag tggtgaaagt cctcagaccc acaccgagag gagcggtgat ggatatcata 9240 tccaggaaag accaaagagg tagtggacaa gttggaacat atggtttgaa cacattcacc 9300 aacatggaag ttcaactcat ccgccaaatg gaagctgaag gagtcatcac acaagatgac 9360 atgcagaacc caaaagggtt gaaagaaaga gttgagaaat ggctgaaaga gtgtggtgtc 9420 gacaggttaa agaggatggc aatcagtgga gacgattgcg tggtgaagcc cctggatgag 9480 aggtttggca cttccctcct cttcttgaac gacatgggaa aggtgaggaa agacattccg 9540 cagtgggaac catctaaggg atggaaaaac tggcaagagg ttcctttttg ctcccaccac 9600 tttcacaaga tcttcatgaa ggatggccgc tcactagttg ttccatgtag aaaccaggat 9660 gaactgatag ggagagccag aatctcgcag ggggctggat ggagcttaag agaaacagcc 9720 tgcctgggca aagcttacgc ccagatgtgg tcgctcatgt acttccacag aagggatctg 9780 cgtttagcct ccatggccat atgctcagca gttccaacgg aatggtttcc aacaagcaga 9840 acaacatggt caatccacgc ycatcatcag tggatgacca ctgaagatat gctcaaagtg 9900 tggaacagag tgtggataga agacaaccct aatatgactg acaagactcc agtccattcg 9960 tgggaagata taccttacct agggaaaaga gaggatttgt ggtgtggatc cctgattgga 10020 ctttcttcca gagccacctg ggcgaagaac attcacacgg ccataaccca ggtcagraac 10080 ctgatcggaa aagaggaata cgtggattac atgccagtaa tgaaaagata cagcgctcct 10140 tcagagagtg aaggagttct g 10161

Claims (50)

  1. 약독화 혈청형 1 뎅기 바이러스 (DENV1) 혼합물, 약독화 혈청형 2 뎅기 바이러스 (DENV2) 혼합물, 약독화 혈청형 3 뎅기 바이러스 (DENV3) 혼합물 및 약독화 혈청형 4 뎅기 바이러스 (DENV4) 혼합물을 포함하는 약독화 4가 뎅기 바이러스 (DENV) 백신 조성물로서, 상기 백신은 하기 특징을 가지는 약독화 4가 뎅기 바이러스 (DENV) 백신 조성물:
    (a) 상기 약독화 DENV1 혼합물은 K482E/K, E483K, K484R/K, K568R, N1663K, I/T2353T 및 A2364T/A 돌연변이를 가지는 DENV1 바이러스를 포함하고; 여기서 넘버링은 서열번호 1을 포함하는 DENV1 친주 03135에 기초하고;
    (b) 상기 약독화 DENV2 혼합물은 D143N, T400K, D1102N, L1308F, E1654K, P2347P/L 및 T2828T/M 돌연변이를 가지는 DENV2 바이러스를 포함하고; 여기서 넘버링은 서열번호 2를 포함하는 DENV2 친주 99345에 기초하고;
    (c) 상기 약독화 DENV3 혼합물은 I209L, S582G, K/R671K, A687V, T764I/T, F1211L, A1237T 및 Q1563K 돌연변이를 가지는 DENV3 바이러스를 포함하고; 여기서 넘버링은 서열번호 3을 포함하는 DENV3 친주 16562에 기초하고;
    (d) 상기 약독화 DENV4 혼합물은 L2187F 및 F/L2354S 돌연변이를 가지는 DENV4 바이러스를 포함하고; 여기서 넘버링은 서열번호 4를 포함하는 DENV4 친주 1036에 기초함.
  2. 제1항에 있어서, 약독화 혈청형 1, 2, 3 및 4 뎅기 바이러스의 혼합비가 1:1:1:1, 5:3:5:3 또는 5:3:3:3인, 약독화 4가 뎅기 바이러스 백신 조성물.
  3. 제1항에 있어서, 약독화 혈청형 1, 2, 3 및 4 뎅기 바이러스의 각 항원량이 1 내지 7 log 10 FFU/dose인, 약독화 4가 뎅기 바이러스 백신 조성물.
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KR1020187017891A 2015-11-27 2016-11-25 뎅기 바이러스 약독주를 뱅크화한 생바이러스, 및 그것들을 항원으로 하는 뎅기 백신 KR102297300B1 (ko)

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