KR102257305B1 - 미세유동 장치 및 분비물의 다세포 검정법에서의 그의 사용 방법 - Google Patents

미세유동 장치 및 분비물의 다세포 검정법에서의 그의 사용 방법 Download PDF

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KR102257305B1
KR102257305B1 KR1020157031147A KR20157031147A KR102257305B1 KR 102257305 B1 KR102257305 B1 KR 102257305B1 KR 1020157031147 A KR1020157031147 A KR 1020157031147A KR 20157031147 A KR20157031147 A KR 20157031147A KR 102257305 B1 KR102257305 B1 KR 102257305B1
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KR20150134429A (ko
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마르게타 리치코바
케빈 알버트 헤이리에스
한스 잔
올레 페트리브
베로니크 레카울트
아누팜 싱할
다니엘 제이. 다 코스타
칼 엘. 지. 한센
브래드 넬슨
줄리 닐슨
캐슬린 리사인고
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더 유니버시티 오브 브리티쉬 콜롬비아
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    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
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KR1020157031147A 2013-03-28 2014-03-28 미세유동 장치 및 분비물의 다세포 검정법에서의 그의 사용 방법 Active KR102257305B1 (ko)

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KR1020217015445A KR102402451B1 (ko) 2013-03-28 2014-03-28 미세유동 장치 및 분비물의 다세포 검정법에서의 그의 사용 방법

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US201361806329P 2013-03-28 2013-03-28
US61/806,329 2013-03-28
PCT/CA2014/000304 WO2014153651A1 (en) 2013-03-28 2014-03-28 Microfluidic devices and methods for use thereof in multicellular assays of secretion

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KR102257305B1 true KR102257305B1 (ko) 2021-05-28

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KR1020157031147A Active KR102257305B1 (ko) 2013-03-28 2014-03-28 미세유동 장치 및 분비물의 다세포 검정법에서의 그의 사용 방법
KR1020227017252A Active KR102668221B1 (ko) 2013-03-28 2014-03-28 미세유동 장치 및 분비물의 다세포 검정법에서의 그의 사용 방법
KR1020217015445A Active KR102402451B1 (ko) 2013-03-28 2014-03-28 미세유동 장치 및 분비물의 다세포 검정법에서의 그의 사용 방법

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KR1020217015445A Active KR102402451B1 (ko) 2013-03-28 2014-03-28 미세유동 장치 및 분비물의 다세포 검정법에서의 그의 사용 방법

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US (3) US10725024B2 (https=)
EP (2) EP2978855B1 (https=)
JP (4) JP6608803B2 (https=)
KR (3) KR102257305B1 (https=)
CN (3) CN105492621A (https=)
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Families Citing this family (129)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691145B2 (en) 2009-11-16 2014-04-08 Flodesign Sonics, Inc. Ultrasound and acoustophoresis for water purification
US9272234B2 (en) 2012-03-15 2016-03-01 Flodesign Sonics, Inc. Separation of multi-component fluid through ultrasonic acoustophoresis
US9950282B2 (en) 2012-03-15 2018-04-24 Flodesign Sonics, Inc. Electronic configuration and control for acoustic standing wave generation
US10689609B2 (en) 2012-03-15 2020-06-23 Flodesign Sonics, Inc. Acoustic bioreactor processes
US9745548B2 (en) 2012-03-15 2017-08-29 Flodesign Sonics, Inc. Acoustic perfusion devices
US9567559B2 (en) 2012-03-15 2017-02-14 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US9796956B2 (en) 2013-11-06 2017-10-24 Flodesign Sonics, Inc. Multi-stage acoustophoresis device
US10322949B2 (en) 2012-03-15 2019-06-18 Flodesign Sonics, Inc. Transducer and reflector configurations for an acoustophoretic device
US10967298B2 (en) 2012-03-15 2021-04-06 Flodesign Sonics, Inc. Driver and control for variable impedence load
US9752114B2 (en) 2012-03-15 2017-09-05 Flodesign Sonics, Inc Bioreactor using acoustic standing waves
US9783775B2 (en) 2012-03-15 2017-10-10 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
US10370635B2 (en) 2012-03-15 2019-08-06 Flodesign Sonics, Inc. Acoustic separation of T cells
US9752113B2 (en) 2012-03-15 2017-09-05 Flodesign Sonics, Inc. Acoustic perfusion devices
US9458450B2 (en) 2012-03-15 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic separation technology using multi-dimensional standing waves
US10953436B2 (en) 2012-03-15 2021-03-23 Flodesign Sonics, Inc. Acoustophoretic device with piezoelectric transducer array
US10737953B2 (en) 2012-04-20 2020-08-11 Flodesign Sonics, Inc. Acoustophoretic method for use in bioreactors
US9857333B2 (en) 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
US9745569B2 (en) 2013-09-13 2017-08-29 Flodesign Sonics, Inc. System for generating high concentration factors for low cell density suspensions
US9889445B2 (en) * 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
EP3783094B1 (en) 2013-10-22 2023-10-11 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
EP3760703A1 (en) 2013-10-22 2021-01-06 Berkeley Lights, Inc. Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
US11318479B2 (en) 2013-12-18 2022-05-03 Berkeley Lights, Inc. Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device
US9610579B2 (en) 2014-01-07 2017-04-04 Daktari Diagnostics, Inc. Fluid delivery devices, systems, and methods
WO2015105955A1 (en) 2014-01-08 2015-07-16 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
US11192107B2 (en) 2014-04-25 2021-12-07 Berkeley Lights, Inc. DEP force control and electrowetting control in different sections of the same microfluidic apparatus
EP3517625B1 (en) 2014-05-22 2021-01-20 The University of British Columbia Methods for determining lymphocyte receptor chain pairs
WO2015188171A1 (en) 2014-06-06 2015-12-10 Berkeley Lights, Inc. Isolating microfluidic structures and trapping bubbles
US9744483B2 (en) 2014-07-02 2017-08-29 Flodesign Sonics, Inc. Large scale acoustic separation device
US20160123975A1 (en) * 2014-11-03 2016-05-05 Daktari Diagnostics, Inc. Mesh Microfluidic Mixing Chamber
US10584366B2 (en) 2014-12-03 2020-03-10 IsoPlexis Corporation Analysis and screening of cell secretion profiles
EP3229961B1 (en) 2014-12-08 2019-11-13 Berkeley Lights, Inc. Actuated microfluidic structures for directed flow in a microfluidic device and methods of use thereof
EP3230718B1 (en) 2014-12-09 2022-03-02 Berkeley Lights, Inc. Automated detection of assay-positive areas or of analyte quantities in microfluidic devices
WO2016094715A2 (en) 2014-12-10 2016-06-16 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
TWI700125B (zh) 2014-12-10 2020-08-01 美商柏克萊燈光有限公司 用於操作電氣動力裝置之系統
US11353448B2 (en) * 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
CN116064731A (zh) * 2015-03-13 2023-05-05 哈佛学院院长及董事 使用扩增测定细胞
US10106770B2 (en) 2015-03-24 2018-10-23 Flodesign Sonics, Inc. Methods and apparatus for particle aggregation using acoustic standing waves
US10590461B2 (en) 2015-04-21 2020-03-17 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods using magnetic beads for high throughput microbiology applications
SG11201708429WA (en) 2015-04-22 2017-11-29 Berkeley Lights Inc Microfluidic cell culture
US10751715B1 (en) 2015-04-22 2020-08-25 Berkeley Lights, Inc. Microfluidic reporter cell assay methods and kits thereof
US10101250B2 (en) 2015-04-22 2018-10-16 Berkeley Lights, Inc. Manipulation of cell nuclei in a micro-fluidic device
CN107810059B (zh) 2015-04-22 2021-03-23 伯克利之光生命科技公司 在微流体装置上冷冻和存档细胞
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11021699B2 (en) 2015-04-29 2021-06-01 FioDesign Sonics, Inc. Separation using angled acoustic waves
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
CA3005845A1 (en) * 2015-07-28 2017-02-02 Flodesign Sonics, Inc. Acoustic affinity separation
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
CN108495712A (zh) 2015-11-23 2018-09-04 伯克利之光生命科技公司 原位生成的微流体隔离结构、试剂盒及其使用方法
US10705082B2 (en) 2015-12-08 2020-07-07 Berkeley Lights, Inc. In situ-generated microfluidic assay structures, related kits, and methods of use thereof
EP3394326B1 (en) * 2015-12-21 2022-09-14 The University Of British Columbia Highly parallel assays for simultaneous identification of antibody sequences and binding partners
CA3009073C (en) 2015-12-30 2024-11-12 Berkeley Lights, Inc. MICROFLUID DEVICES FOR OPTICALLY CONTROLLED CONVECTION AND MOVEMENT, KITS AND ASSOCIATED PROCESSES
WO2017117521A1 (en) 2015-12-31 2017-07-06 Berkeley Lights, Inc. Tumor infilitrating cells engineered to express a pro-inflammatory polypeptide
JP7179329B2 (ja) * 2016-01-14 2022-11-29 ヨーロピアン モレキュラー バイオロジー ラボラトリー リガンド誘発型の細胞発現のマイクロ流体分析
EP3889176A1 (en) 2016-01-15 2021-10-06 Berkeley Lights, Inc. Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor
US10962527B2 (en) 2016-02-05 2021-03-30 The Broad Institute, Inc. Multi-stage, multiplexed target isolation and processing from heterogeneous populations
CN109922885B (zh) 2016-03-16 2022-05-10 伯克利之光生命科技公司 用于基因组编辑克隆的选择和传代的方法、系统和装置
CN109154017B (zh) * 2016-03-17 2023-07-18 不列颠哥伦比亚大学 细胞分泌分析的装置和方法
EP4684881A3 (en) 2016-04-15 2026-03-18 Bruker Cellular Analysis, Inc. Methods, systems, computer program and non-transitory computer-readable medium for in-pen assays
US10675625B2 (en) 2016-04-15 2020-06-09 Berkeley Lights, Inc Light sequencing and patterns for dielectrophoretic transport
US20190127789A1 (en) 2016-04-19 2019-05-02 President And Fellows Of Harvard College Immobilization-based systems and methods for genetic analysis and other applications
US10940476B2 (en) 2016-04-22 2021-03-09 Arizona Board Of Regents On Behalf Of Arizona State University Device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation
US10710006B2 (en) 2016-04-25 2020-07-14 Flodesign Sonics, Inc. Piezoelectric transducer for generation of an acoustic standing wave
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
US11085035B2 (en) 2016-05-03 2021-08-10 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
CN109715124B (zh) 2016-05-03 2022-04-22 弗洛设计声能学公司 利用声泳的治疗细胞洗涤、浓缩和分离
CN115678773A (zh) 2016-05-26 2023-02-03 伯克利之光生命科技公司 共价修饰的表面、试剂盒及制备方法和用途
CA3027166A1 (en) * 2016-06-30 2018-01-04 General Automation Lab Technologies, Inc. High resolution systems, kits, apparatus, and methods using magnetic beads for high throughput microbiology applications
AU2017298545B2 (en) 2016-07-21 2022-10-27 Berkeley Lights, Inc. Sorting of T lymphocytes in a microfluidic device
WO2018049418A1 (en) 2016-09-12 2018-03-15 IsoPlexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
WO2018053119A2 (en) 2016-09-15 2018-03-22 Augmenta Bioworks, Inc. Immune repertoire sequence amplification methods and applications
WO2018075830A1 (en) 2016-10-19 2018-04-26 Flodesign Sonics, Inc. Affinity cell extraction by acoustics
CN106520537B (zh) * 2016-10-21 2018-10-23 南通大学 一种t细胞免疫应答的微流控光学分析系统及分析方法
EP3538891B1 (en) 2016-11-11 2022-01-05 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
CA3046200A1 (en) 2016-12-06 2018-06-14 Brandeis University Freezable fluid cell for cryo-electron microscopy
CN110546495B (zh) 2016-12-30 2022-11-01 加利福尼亚州立大学董事会 用于基因组编辑t细胞的选择和传代的方法
TWI832820B (zh) 2017-07-21 2024-02-21 美商伯克利之光生命科技公司 抗原呈現合成表面、共價功能化表面、經活化之t細胞及其用途
WO2019069224A1 (en) * 2017-10-02 2019-04-11 Khalifa University of Science and Technology MICROFLUIDIC DEVICE FOR GENERATING IN VITRO LYMPHATIC GANGLION
JP7209701B2 (ja) * 2017-10-03 2023-01-20 アベイルズ メディカル，インコーポレイテッド レドックス反応に基づいて微生物の濃度及び抗感染剤に対する微生物の感受性を決定する装置、システム、及び方法
EP3721209B1 (en) 2017-10-15 2024-02-07 Berkeley Lights, Inc. Methods for in-pen assays
WO2019079725A1 (en) 2017-10-19 2019-04-25 Georgia Tech Research Corporation MESOFLUIDIC DEVICE FOR CULTIVATION OF CELLULAR AGGREGATES
KR102439221B1 (ko) 2017-12-14 2022-09-01 프로디자인 소닉스, 인크. 음향 트랜스듀서 구동기 및 제어기
WO2019176275A1 (ja) * 2018-03-12 2019-09-19 ソニー株式会社 細胞評価装置及び細胞評価システム
US11433139B2 (en) 2018-03-16 2022-09-06 Zoetis Services Llc Peptide vaccines against interleukin-31
CA3093539A1 (en) 2018-03-16 2019-09-19 Zoetis Services Llc Interleukin-31 monoclonal antibodies for veterinary use
WO2019191321A1 (en) * 2018-03-28 2019-10-03 10X Genomics, Inc. Nucleic acid enrichment within partitions
JP7337438B2 (ja) * 2018-03-30 2023-09-04 エクソソミクス・ソシエタ・ペル・アチオニ 血球および血小板由来の細胞外小胞を困窮化させた血液または血液誘導体を得るための中空糸の使用
US20210054324A1 (en) * 2018-04-05 2021-02-25 University Of Virginia Patent Foundation 3d spatially patterned lymph node on a microfluidic device
US11666914B2 (en) * 2018-05-09 2023-06-06 Tecan Trading Ag Cartridge, electrowetting sample processing system and bead manipulation method
CN110499251A (zh) * 2018-05-17 2019-11-26 中国科学院大连化学物理研究所 高通量多参数的单细胞源性细胞外囊泡分析芯片及应用
EP3849705A4 (en) * 2018-09-14 2022-06-01 Berkeley Lights, Inc. Methods for assaying binding affinity
US11993766B2 (en) 2018-09-21 2024-05-28 Bruker Cellular Analysis, Inc. Functionalized well plate, methods of preparation and use thereof
WO2020077017A2 (en) 2018-10-10 2020-04-16 Augmenta Bioworks, Inc. Methods for isolating immune binding proteins
WO2020077274A1 (en) 2018-10-11 2020-04-16 Berkeley Lights, Inc. Systems and methods for identification of optimized protein production and kits therefor
CN109576345A (zh) * 2018-10-17 2019-04-05 西人马（厦门）科技有限公司 一种用于dna提取的微流控芯片及其检测方法
KR20210078526A (ko) 2018-10-18 2021-06-28 버클리 라잇츠, 인크. 원 항원 제시 합성 표면, 활성화된 t 세포, 및 이들의 용도
CN113366312A (zh) * 2018-11-01 2021-09-07 伯克利之光生命科技公司 在微流体环境中测定生物细胞的方法
GB201820617D0 (en) * 2018-12-18 2019-01-30 Greindx As System for capturing cells
WO2020168258A1 (en) 2019-02-15 2020-08-20 Berkeley Lights, Inc. Laser-assisted repositioning of a micro-object and culturing of an attachment-dependent cell in a microfluidic environment
EP3941631A4 (en) * 2019-03-22 2022-12-14 Augmenta Bioworks, Inc. ISOLATION OF SINGLE CELLS AND THEIR USES
KR102871135B1 (ko) * 2019-04-08 2025-10-14 엑셀라 바이오사이언시스 인코포레이티드 미세모세관 어레이를 이용하는 스크리닝 방법 및 시스템
CN110006866B (zh) * 2019-04-16 2022-04-22 杭州迈尔德生物科技有限公司 一种阿片类活性物质的通用检测方法及其检测试剂盒
WO2020223555A1 (en) 2019-04-30 2020-11-05 Berkeley Lights, Inc. Methods for encapsulating and assaying cells
MX2021014439A (es) 2019-05-28 2022-05-19 Xilis Inc Metodos y aparatos para microorganoesferas derivadas de pacientes.
US20200392578A1 (en) * 2019-06-14 2020-12-17 The Regents Of The University Of California Methods of sequencing antibody chains from hybridomas and kits for practicing same
JP7607573B2 (ja) * 2019-09-30 2024-12-27 東京応化工業株式会社 分泌物産生細胞のスクリーニング方法、及び分泌物産生細胞のスクリーニングキット
WO2021097456A1 (en) * 2019-11-17 2021-05-20 The Regents Of The University Of California Guided Microfluidic Flow for Cell Capture, Indexing, and Directed Release
AU2020388676B2 (en) * 2019-11-21 2024-07-18 Emulate Inc. Antibody producing microfluidic devices
CN112557475B (zh) * 2020-02-19 2021-07-06 南京启医科技有限公司 一种基于微流控和elisa分析的外泌体分离检测系统
CN113373104B (zh) * 2020-03-09 2022-11-18 浙江大学 一种用于稀有细胞高纯度分选的装置及方法
CN114410466A (zh) * 2020-04-01 2022-04-29 沈西凌 用于个性化癌症治疗的精确药物筛选
CN113529075B (zh) * 2020-04-20 2022-05-03 厦门大学 一种液态金属复合多孔膜及其制备方法和应用
US20230182135A1 (en) * 2020-05-14 2023-06-15 Bifrost Biosystems A microfluidic device having specifically designed detection chambers
SMT202500106T1 (it) 2020-08-18 2025-05-12 Cephalon Llc Anticorpi anti-par-2 e loro metodi d'uso
TWI729914B (zh) * 2020-08-18 2021-06-01 國立臺灣海洋大學 鮮度指示裝置
NL2026383B1 (en) * 2020-08-31 2022-04-29 Lumicks Ca Holding B V Method and system for studying objects, in particular biological cells
CA3210091A1 (en) * 2021-02-05 2022-08-11 Amgen Inc. Non-terminal antibody discovery methods and single cell assays
WO2022241202A1 (en) * 2021-05-14 2022-11-17 The Texas A&M University System A platform for the fast, label-free, automated evaluation of sterility and bioburden
EP4526355A1 (en) * 2022-05-19 2025-03-26 Triplebar Bio, Inc. Multiparametric discovery and optimization platform
CN115735846B (zh) * 2022-11-29 2025-09-16 复旦大学附属华山医院 磁场联合光化学诱导无创性缺血性脑卒中模型的建立方法
WO2024130036A1 (en) * 2022-12-14 2024-06-20 President And Fellows Of Harvard College Systems and methods for identifying gpcr modulators and other agents
CN116237099B (zh) * 2023-03-16 2025-07-25 东南大学 线虫原位电阻抗谱检测的微流控芯片
KR102543112B1 (ko) * 2023-04-12 2023-06-13 주식회사 브레디스헬스케어 유체 시료 내에 극미량으로 존재하는 분석 대상 물질의 입자를 검출하기 위한 방법
CN116626293A (zh) * 2023-06-08 2023-08-22 复旦大学 一种用于检测溶组织内阿米巴感染的微流控芯片
WO2025111584A1 (en) * 2023-11-22 2025-05-30 Georgia Tech Research Corporation An electronic microfluidic platform for on-chip apoptosis quantification using annexin v-based ps externalization detection
WO2025182763A1 (ja) * 2024-02-29 2025-09-04 富士レビオ株式会社 検出方法、検出装置、並びに、これらに用いる処理液及びキット
CN118731352B (zh) * 2024-09-02 2024-12-06 天津锐尔康生物科技有限公司 用于细胞表面标志物的自动化分析检测方法及系统

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120015347A1 (en) 2010-07-16 2012-01-19 Anupam Singhal Methods for Assaying Cellular Binding Interactions

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6008010A (en) * 1996-11-01 1999-12-28 University Of Pittsburgh Method and apparatus for holding cells
DE19725190A1 (de) * 1997-06-14 1998-12-17 Innova Gmbh Vorrichtungen mit integrierten Elektroden aus elektrisch leitfähigen Kunststoffen
US6740497B2 (en) * 1998-03-06 2004-05-25 The Regents Of The University Of California Method and apparatus for detecting cancerous cells using molecules that change electrophoretic mobility
EP1581648A2 (en) 2002-09-09 2005-10-05 Nura, Inc. G protein coupled receptors and uses thereof
WO2006042177A2 (en) * 2004-10-07 2006-04-20 Argos Therapeutics, Inc. Mature dendritic cell compositions and methods for culturing same
WO2006060748A2 (en) 2004-12-03 2006-06-08 California Institute Of Technology Microfluidic sieve valves
US8796020B2 (en) 2005-06-17 2014-08-05 Inha-Industry Partnership Institute Manufacturing process for fresh and frozen stem cells
WO2008143910A2 (en) * 2007-05-18 2008-11-27 University Of Massachusetts A strategy for cloning and expressing the extracellular domains of receptors as soluble proteins
WO2009111014A2 (en) 2008-03-04 2009-09-11 Crystal Bioscience Inc. Gel microdrop composition and method of using the same
WO2010005593A1 (en) * 2008-07-11 2010-01-14 President And Fellows Of Harvard College Systems and methods of droplet-based selection
GB2464300A (en) 2008-10-10 2010-04-14 Univ Dublin City Microfluidic multiplexed cellular and molecular analysis device and method
US10087408B2 (en) 2010-07-07 2018-10-02 The University Of British Columbia System and method for microfluidic cell culture
EP2646798B1 (en) 2010-12-03 2017-06-28 Cellply S.R.L. Microanalysis of cellular function
US9902990B2 (en) 2011-05-27 2018-02-27 The University Of British Columbia Microfluidic cell trap and assay apparatus for high-throughput analysis
US9250229B2 (en) * 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120015347A1 (en) 2010-07-16 2012-01-19 Anupam Singhal Methods for Assaying Cellular Binding Interactions

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Lecault et al., Current Opinion in Chemical Biology, Vol. 16(3-4), 2012, pp. 381-390.
Romanuik et al., Journal of Medical and Biological Engineering, Vol. 31, No. 2, 2011, pp. 121-127.
Singhal et al., 15th International Conference On Miniature Systems For Chemistry and Life Sciences, 2011, pp. 323-325.

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