KR102240515B1 - Cultivating method of wild ginseng root using the same - Google Patents

Abstract

The present invention relates to a method for producing wild ginseng cultured roots. The method comprises the steps of: (S100) preparing natural wild ginseng pieces by cutting natural wild ginsengs; (S200) inoculating the wild ginseng pieces into a medium for culturing wild ginsengs, and primarily culturing the same to grow the same into a wild ginseng cell mass; (S300) inducing irregular roots by further growing the wild ginseng cell mass; (S400) selecting roots with excellent traits from among the irregular roots, and secondarily culturing the same to obtain a fine ginseng root; (S500) adding the fine ginseng root into a bioreactor, and thirdly culturing the same; and (S600) adding the fine ginseng root grown in the bioreactor into a large culture tank, and fourthly culturing the same. A ratio of zinc (Zn), calcium (Ca), and sulfur (S) contained in the produced wild ginseng cultured root can be effectively improved. Also, a cultured wild ginseng having the same ingredients as those of a natural wild ginseng can be obtained with a high yield.

Description

Manufacturing method of cultured wild ginseng root {CULTIVATING METHOD OF WILD GINSENG ROOT USING THE SAME}

The present invention relates to a method for producing a wild ginseng cultured root, and more specifically, a liquid medium for wild ginseng culture that can effectively improve the ratio of zinc (Zn), calcium (Ca) and sulfur (S) contained in the produced wild ginseng cultured root, and It relates to a method for producing cultured wild ginseng roots using this.

Since ancient times, wild ginseng has been recorded in Shinnongbonchogyeong and Donguibogam, and has been recognized as an excellent medicine for longevity.

The quantity of wild ginseng is extremely limited, so it is difficult to popularize wild ginseng because it is traded at a higher price as the older ones are, and it is impossible to standardize the ingredients due to their quality or variety.

Wild ginseng stem cells developed using biotechnology from wild wild ginseng roots and cultured in a sterile state without pesticides, antibiotics, or preservatives in a biological incubator are called wild ginseng cultured roots.

Various culture media are used to prepare these wild ginseng cultured roots.

The following Patent Document 1 discloses a medium for accelerating the growth of wild ginseng cultured roots including calcium chloride, boric acid, zinc sulfate, sodium molybdate, copper sulfate, indolebutyric acid, pyridoxine hydrochloride and thiamine hydrochloride. There was a limitation in that the efficiency of absorbing beneficial ingredients such as (Ca) or sulfur (S) into the cultured wild ginseng muscle was poor.

Therefore, there is an urgent need to develop a novel technology capable of improving the efficiency of absorbing beneficial ingredients such as zinc (Zn), calcium (Ca), or sulfur (S) into the cultured wild ginseng muscle.

Patent Document 1: Korean Patent Registration No. 10-1377545 (2014.03.25)

It is an object of the present invention to provide a method for producing cultured wild ginseng roots that can effectively improve the ratio of zinc (Zn), calcium (Ca) and sulfur (S) contained in the cultured wild ginseng roots to be prepared.

Wild ginseng cultured root production method according to the present invention comprises the steps of preparing a piece of natural wild ginseng by cutting the natural wild ginseng (S100); Inoculating the natural wild ginseng fragments in a wild ginseng culture medium and first culturing them to grow wild ginseng cell masses (S200); Further growing the wild ginseng cell mass to induce adventitious muscles (S300); Selecting a root having excellent traits from among the adventitious roots and secondary culturing the roots to obtain wild ginseng fine roots (S400); Putting the wild ginseng fine roots into a bioreactor and performing a third culture (S500); And a step (S600) of culturing the wild ginseng fine roots grown in the bioreactor into a large culture tank (S600).

In the wild ginseng cultured root production method according to the present invention, the step of harvesting the wild ginseng completed in the fourth culture in a large culture tank and washing with purified water (S700); And drying the washed wild ginseng (S800).

In the wild ginseng cultured root production method according to the present invention, at least one of the first to fourth cultures is _{ammonium nitrate (NH 4} NO ₃ , ammonium nitrate), calcium nitrate tetrahydrate ([CaNO ₃ ] ₂ ·4H ₂ 0 , calcium nitrate tetrahydrate), _{potassium phosphate monobasic (KH 2} PO ₄ , potassium phosphate monobasic), potassium sulfate (K ₂ SO ₄ , potassium sulfate), magnesium sulfate 7 hydrate (MgSO ₄ 7H ₂ O, magnesium sulfate heptahydrate), Anhydrous calcium chloride (CaCl ₂ , calcium chloride anhydrous), boric acid (H ₃ BO ₃ , boric acid), zinc sulfate heptahydrate (ZnSO ₄ 7H ₂ O, zinc sulfate heptahydrate), sodium molybdate dihydrate (Na ₂ Mo0 ₄ 2H ₂ 0, sodium molybdate dihydrate), copper sulfate (CuS0 ₄ 5H ₂ O, copper(I) sulfate pentahydrate), sodium edetate ferric (EDTA ferric sodium salt trihydrate), 3-indolebutyric acid (IBA ( Wild ginseng containing C ₁₂ H ₁₃ NO ₂ ), 3-indolebutyric acid), potassium hydroxide (KOH), inositol, pyridoxine hydrochloride (C ₈ H ₁₁ NO ₃ HCl, pyridoxine hydrochloride) and thiamine hydrochloride It may be performed using a liquid culture medium.

In the wild ginseng baeyanggeun production process according to the invention, the wild ginseng liquid medium for culture is the total liquid medium 12 per liter of ammonium nitrate _{(NH 4 NO 3, ammonium nitrate} ) 3 to 7g, calcium nitrate tetrahydrate ([CaNO _{3] 2} · 4H ₂ 0, calcium nitrate tetrahydrate) 4 to 7 g, _{potassium phosphate (KH 2} PO ₄ , potassium phosphate monobasic) 1 to 3 g, potassium sulfate (K ₂ SO ₄ , potassium sulfate) 10 to 15 g, magnesium sulfate 7 hydrate (MgSO ₄ 7H ₂ O, magnesium sulfate heptahydrate) 1 to 3 g, anhydrous calcium chloride (CaCl ₂ , calcium chloride anhydrous) 0.1 to 2 g, boric acid (H ₃ BO ₃ , boric acid) 0.05 to 0.5 g, zinc sulfate heptahydrate (ZnSO ₄₎ 7H ₂ O, zinc sulfate heptahydrate) 0.05 to 0.5 g, _{sodium molybdate dihydrate (Na 2} Mo0 ₄ 2H ₂ 0, sodium molybdate dihydrate) 0.05 to 0.5 g, copper sulfate, CuS0 ₄ 5H ₂ O, copper (I) sulfate pentahydrate) 0.05 to 0.5 g, sodium _{edetate ferric acid (EDTA ferric sodium salt trihydrate) 0.1 to 2 g, 3-indolebutyric acid (IBA (C 12} H ₁₃ NO ₂ ), 3-indolebutyric acid) 0.05 To 0.5 g, potassium hydroxide (KOH) 0.05 to 0.5 g, inositol 0.5 to 3 g, pyridoxine hydrochloride (C ₈ H ₁₁ NO ₃ HCl, pyridoxine hydrochloride) 0.05 to 0.5 g and thiamine hydrochloride 0.05 to 0.5 It may contain g.

In the wild ginseng cultured root production method according to the present invention, the liquid medium for cultivating wild ginseng is _{ammonium nitrate (NH 4} NO ₃ , ammonium nitrate) 5 g per 12 liters of the total liquid medium, calcium nitrate tetrahydrate ([CaNO ₃ ] ₂ ·4H ₂ 0 , calcium nitrate tetrahydrate) 6.3g, _{potassium monobasic (KH 2} PO ₄ , potassium phosphate monobasic) 2.1g, potassium sulfate (K ₂ SO ₄ , potassium sulfate) 12.5 g, magnesium sulfate 7 hydrate (MgSO ₄ 7H ₂ O , magnesium sulfate heptahydrate) 1.9g, anhydrous calcium chloride _{(CaCl 2, calcium chloride anhydrous)} 0.6g, boric acid _{(H 3 BO 3, boric acid} ) 0.1g, zinc sulfate heptahydrate _{(ZnSO 4 · 7H 2 O,} zinc sulfate heptahydrate) 0.1g, _{sodium molybdate dihydrate (Na 2} Mo0 ₄ ·2H ₂ 0, sodium molybdate dihydrate) 0.1g, copper sulfate (CuS0 ₄ ·5H ₂ O, copper(I) sulfate pentahydrate) 0.1g, sodium edetate Ferric (EDTA ferric sodium salt trihydrate) 0.5g, _{3-indolebutyric acid (IBA(C 12} H ₁₃ NO ₂ ), 3-indolebutyric acid) 0.1g, potassium hydroxide (KOH) 0.1g, inositol 1.3g , Pyridoxine hydrochloride (C ₈ H ₁₁ NO ₃ HCl, pyridoxine hydrochloride) 0.1 g and thiamine hydrochloride (thiamine hydrochloride) 0.1 g.

The method for producing a wild ginseng cultured root according to the present invention can effectively improve the ratio of zinc (Zn), calcium (Ca) and sulfur (S) contained in the cultured wild ginseng It has the advantage of obtaining cultured wild ginseng.

1 is a process diagram showing a schematic process of a method for manufacturing a wild ginseng cultured root according to the present invention.
2 is a photograph showing a process of obtaining cultured wild ginseng roots according to the method for producing cultured wild ginseng roots according to the present invention.
Figure 3 is an electrophoresis picture for comparing the components of natural wild ginseng collected in Korea and the components of cultured wild ginseng prepared according to the present invention.
4 and 5 illustrate the steps of obtaining wild ginseng fine roots.
6 shows a test report that can confirm that the cultured wild ginseng prepared according to the present invention does not contain any pesticide residues.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily implement the present invention. However, the present invention may be implemented in various different forms and is not limited to the embodiments described herein.

The present invention relates to a method for producing a wild ginseng cultured root, and more specifically, a liquid medium for wild ginseng culture that can effectively improve the ratio of zinc (Zn), calcium (Ca) and sulfur (S) contained in the produced wild ginseng cultured root, and It relates to a method for producing cultured wild ginseng roots using this.

The method for producing cultured wild ginseng root according to the present invention has an excellent advantage of effectively improving the content of zinc (Zn), calcium (Ca) and sulfur (S) contained in the cultured wild ginseng root to be prepared.

The amount of wild ginseng produced in Korea using advanced technology from the sky is not accurately understood, but it is estimated that it is a very small amount. In some cases, it is distorted and traded. In addition, research on cultivation of wild ginseng is actively progressing due to the increase in the rarity and value of wild ginseng, and the area is rapidly increasing in recent years, but there is a difficulty in controlling diseases and pests caused by group cultivation.

In particular, camphor ginseng imported from China or North Korea is a problem due to the detection of pesticides. In the case of Jebae ginseng, the area of cultivation is reduced every year due to the problem of serial cultivation, and it is expected to emerge as a serious problem that hinders the stable production of ginseng in the future. In order to solve this problem in the long term, it is intended to popularize the wild ginseng in a factory type without pesticide treatment, which does not damage existing ginseng cultivation farms, and is difficult to access by the general public due to its rarity and high price. In addition, plants that exist in nature are a treasure trove of infinite resources, and more than 25% of the medicines actually used are substances derived from plants.

Until now, only 5,000 plant species are being studied for drug development purposes out of the total 300,000 species.

However, the indiscriminate harvesting of plant resources existing in the natural world poses a risk of depletion of nature and destruction of the environment. (Example: Dixol extraction, which has special effects on ovarian cancer and breast cancer from the attention of the natural mountain Pacific) Therefore, the technology for producing useful substances using bioreactors is a technology that is attracting worldwide attention among future growth engines, as an alternative drug related to human well-being in the future. It can be said to be a source technology with very high functionality that is widely applied to the production of functional food ingredients.

Wild ginseng cultured root production method according to the present invention comprises the steps of preparing a piece of natural wild ginseng by cutting the natural wild ginseng (S100); Inoculating the natural wild ginseng fragments in a wild ginseng culture medium and first culturing them to grow wild ginseng cell masses (S200); Further growing the wild ginseng cell mass to induce adventitious muscles (S300); Selecting a root having excellent traits from among the adventitious roots and secondary culturing the roots to obtain wild ginseng fine roots (S400); Putting the wild ginseng fine roots into a bioreactor and performing a third culture (S500); And a step (S600) of culturing the wild ginseng fine roots grown in the bioreactor into a large culture tank (S600).

The step of preparing a piece of natural wild ginseng by cutting the natural wild ginseng (S100) is a step of selecting 100-year-old natural wild ginseng to be used for cultivation and aseptically cutting it into a predetermined size.

Meanwhile, the step of inoculating and culturing the natural wild ginseng pieces into wild ginseng culture medium to grow them into wild ginseng cell mass (S200) is a process of growing wild ginseng cell mass by planting the selected natural wild ginseng pieces in a culture medium and culturing them in a clean room.

Then, the step of inducing adventitious roots by further growing the wild ginseng cell mass (S300) is performed.

When acquiring the wild ginseng ginseng to be described later through the adventitious root induction step (S300), sufficient ginseng ginseng roots capable of selecting roots having excellent traits may be prepared.

On the other hand, next, the step (S400) of obtaining wild ginseng fine roots by selecting and culturing roots having excellent traits among the adventitious roots is performed.

Using the obtained wild ginseng fine roots, an additional culture process described later is performed.

That is, the step of culturing the wild ginseng fine roots into the bioreactor (S500) is next performed.

Next, the step (S600) of extracting the wild ginseng fine roots cultured in the bioreactor as described above and putting them into a large culture tank is performed (S600).

This is a process of culturing wild ginseng roots grown in a biological incubator in a large culture tank of about 13 tons.

Next, the wild ginseng cultured roots are completed by performing a step (S700) of harvesting the wild ginseng that has been cultured in a large culture tank and washing with purified water (S700), and finally performing a step (S800) of drying the washed wild ginseng.

On the other hand, in the wild ginseng cultured root production method according to the present invention, any one or more of the first culture to the fourth culture is _{ammonium nitrate (NH 4} NO ₃ , ammonium nitrate), calcium nitrate tetrahydrate ([CaNO ₃ ] ₂ ·4H ₂ 0, calcium nitrate tetrahydrate), _{potassium phosphate monobasic (KH 2} PO ₄ , potassium phosphate monobasic), potassium sulfate (K ₂ SO ₄ , potassium sulfate), magnesium sulfate heptahydrate (MgSO ₄ 7H ₂ O, magnesium sulfate heptahydrate) ), anhydrous calcium chloride (CaCl ₂ , calcium chloride anhydrous), boric acid (H ₃ BO ₃ , boric acid), zinc sulfate heptahydrate (ZnSO ₄ 7H ₂ O, zinc sulfate heptahydrate), sodium molybdate dihydrate (Na ₂ Mo0 ₄ 2H ₂ 0, sodium molybdate dihydrate), copper sulfate (CuS0 ₄ 5H ₂ O, copper(I) sulfate pentahydrate), sodium edetate ferric (EDTA ferric sodium salt trihydrate), 3-indolebutyric acid ( IBA (C ₁₂ H ₁₃ NO ₂ ), 3-indolebutyric acid), potassium hydroxide (KOH), inositol, pyridoxine hydrochloride (C ₈ H ₁₁ NO ₃ HCl, pyridoxine hydrochloride) and thiamine hydrochloride It is possible to carry out using a liquid medium for cultivating wild ginseng.

That is, the present inventors have found that when wild ginseng is cultured using a medium composed of these components, cultured wild ginseng having almost the same components as natural wild ginseng can be easily obtained in large quantities.

In the wild ginseng cultured root production method according to the present invention, the liquid medium for cultivating wild ginseng is _{ammonium nitrate (NH 4} NO ₃ , ammonium nitrate) 3 to 7 g _{per 12 liters of the total liquid medium, calcium nitrate tetrahydrate ([CaNO 3} ] ₂ ·4H ₂ 0, calcium nitrate tetrahydrate) 4 to 7 g, _{potassium phosphate (KH 2} PO ₄ , potassium phosphate monobasic) 1 to 3 g, potassium sulfate (K ₂ SO ₄ , potassium sulfate) 10 to 15 g, magnesium sulfate 7 hydrate (MgSO ₄ 7H ₂ O, magnesium sulfate heptahydrate) 1 to 3 g, anhydrous calcium chloride (CaCl ₂ , calcium chloride anhydrous) 0.1 to 2 g, boric acid (H ₃ BO ₃ , boric acid) 0.05 to 0.5 g, zinc sulfate heptahydrate (ZnSO ₄₎ 7H ₂ O, zinc sulfate heptahydrate) 0.05 to 0.5 g, _{sodium molybdate dihydrate (Na 2} Mo0 ₄ 2H ₂ 0, sodium molybdate dihydrate) 0.05 to 0.5 g, copper sulfate, CuS0 ₄ 5H ₂ O, copper (I) sulfate pentahydrate) 0.05 to 0.5 g, sodium _{edetate ferric acid (EDTA ferric sodium salt trihydrate) 0.1 to 2 g, 3-indolebutyric acid (IBA (C 12} H ₁₃ NO ₂ ), 3-indolebutyric acid) 0.05 To 0.5 g, potassium hydroxide (KOH) 0.05 to 0.5 g, inositol 0.5 to 3 g, pyridoxine hydrochloride (C ₈ H ₁₁ NO ₃ HCl, pyridoxine hydrochloride) 0.05 to 0.5 g and thiamine hydrochloride 0.05 to 0.5 It may contain g.

More preferably, the liquid medium for cultivation of wild ginseng is _{ammonium nitrate (NH 4} NO ₃ , ammonium nitrate) 5 g _{, calcium nitrate tetrahydrate ([CaNO 3} ] ₂ ·4H ₂ 0, calcium nitrate tetrahydrate) 6.3 per 12 liters of the total liquid medium. g, _{potassium phosphate (KH 2} PO ₄ , potassium phosphate monobasic) 2.1 g, potassium sulfate (K ₂ SO ₄ , potassium sulfate) 12.5 g, magnesium sulfate heptahydrate (MgSO ₄ 7H ₂ O, magnesium sulfate heptahydrate) 1.9 g, anhydrous calcium chloride (CaCl ₂ , calcium chloride anhydrous) 0.6 g, boric acid (H ₃ BO ₃ , boric acid) 0.1 g, zinc sulfate heptahydrate (ZnSO ₄ 7H ₂ O, zinc sulfate heptahydrate) 0.1 g, sodium molybdate Dihydrate (Na _{2 Mo0 4} ·2H ₂ 0, sodium molybdate dihydrate) 0.1g, copper _{sulfate pentahydrate (CuS0 4} ·5H ₂ O, copper(I) sulfate pentahydrate) 0.1g, sodium edetate ferric sodium (EDTA ferric sodium salt trihydrate) 0.5g, _{3-indolebutyric acid (IBA(C 12} H ₁₃ NO ₂ ), 3-indolebutyric acid) 0.1 g, potassium hydroxide (KOH) 0.1 g, inositol 1.3 g, pyridoxine hydrochloride (C ₈ It _{may contain 0.1 g of H 11} NO ₃ HCl, pyridoxine hydrochloride) and 0.1 g of thiamine hydrochloride.

In particular, the liquid medium for cultivating wild ginseng according to the present invention may further contain zinc (Zn) and/or calcium (Ca) to be absorbed in the cultivated wild ginseng, in this case, zinc (Zn) or calcium per 12 liters of the total liquid medium It is possible to include about 3 to 8 g, preferably about 5 g of (Ca).

1 and 2 are block diagrams and photographs showing a process diagram showing a process of obtaining cultured wild ginseng roots according to the method for producing cultured wild ginseng roots according to the present invention.

Hereinafter, examples of the present invention will be described in comparison with various comparative examples.

Manufacturing Example 1

Ammonium nitrate (NH ₄ NO ₃ , ammonium nitrate) 5g, _{calcium nitrate tetrahydrate ([CaNO 3} ] ₂ ·4H ₂ 0, calcium nitrate tetrahydrate) 6.3g _{, potassium phosphate (KH 2} PO ₄ , potassium phosphate monobasic) 2.1 g, potassium sulfate (K ₂ SO ₄ , potassium sulfate) 12.5 g, magnesium sulfate heptahydrate (MgSO ₄ 7H ₂ O, magnesium sulfate heptahydrate) 1.9 g, anhydrous calcium chloride (CaCl ₂ , calcium chloride anhydrous) 0.6 g, boric acid ( H ₃ BO ₃ , boric acid) 0.1 g, zinc sulfate heptahydrate (ZnSO ₄ 7H ₂ O, zinc sulfate heptahydrate) 0.1 g _{, sodium molybdate dihydrate (Na 2} Mo0 ₄ 2H ₂ 0, sodium molybdate dihydrate) 0.1 g, copper _{sulfate pentahydrate (CuS0 4} 5H ₂ O, copper(I) sulfate pentahydrate) 0.1 g, sodium edetate ferric sodium salt trihydrate 0.5 g, 3-indolebutyric acid (IBA(C ₁₂ H ₁₃ NO ₂ ), 3-indolebutyric acid) 0.1 g, potassium hydroxide (KOH) 0.1 g, inositol 1.3 g, pyridoxine hydrochloride (C ₈ H ₁₁ NO ₃ HCl, pyridoxine hydrochloride) 0.1 g and thiamine hydrochloride ) 0.1g, 5g of zinc (Zn) and 5g of calcium (Ca) were added to purified water to prepare a liquid medium so that the total volume was 12 liters.

Preparation Example 2-Without ammonium nitrate

Calcium nitrate tetrahydrate ([CaNO ₃ ] ₂ ·4H ₂ 0, calcium nitrate tetrahydrate) 6.3 g _{, potassium monobasic (KH 2} PO ₄ , potassium phosphate monobasic) 2.1 g _{, potassium sulfate (K 2} SO ₄ , potassium sulfate) 12.5g, magnesium sulfate heptahydrate _{(MgSO 4 · 7H 2 O,} magnesium sulfate heptahydrate) 1.9g, anhydrous calcium chloride _{(CaCl 2, calcium chloride anhydrous)} 0.6g, boric acid _{(H 3 BO 3, boric acid} ) 0.1g, zinc sulfate Heptahydrate (ZnSO ₄ 7H ₂ O, zinc sulfate heptahydrate) 0.1g _{, sodium molybdate dihydrate (Na 2} Mo0 ₄ 2H ₂ 0, sodium molybdate dihydrate) 0.1g, copper sulfate hydrate (CuS0 ₄ 5H ₂ O, copper(I) sulfate pentahydrate) 0.1g, sodium edetate ferric (EDTA ferric sodium salt trihydrate) 0.5g _{, 3-indolebutyric acid (IBA(C 12} H ₁₃ NO ₂ ), 3-indolebutyric acid) 0.1 g, Potassium hydroxide (KOH) 0.1 g, inositol 1.3 g, pyridoxine hydrochloride (C ₈ H ₁₁ NO ₃ HCl, pyridoxine hydrochloride) 0.1 g and thiamine hydrochloride 0.1 g, zinc (Zn) 5 g and calcium (Ca ) 5g was added to purified water to prepare a liquid medium so that the total volume was 12 liters.

Preparation Example 3-Without calcium nitrate tetrahydrate

Ammonium nitrate (NH ₄ NO ₃ , ammonium nitrate) 5 g, _{potassium monobasic (KH 2} PO ₄ , potassium phosphate monobasic) 2.1 g _{, potassium sulfate (K 2} SO ₄ , potassium sulfate) 12.5 g, magnesium sulfate 7 hydrate (MgSO ₄ 7H ₂ O, magnesium sulfate heptahydrate) 1.9 g, anhydrous calcium chloride (CaCl ₂ , calcium chloride anhydrous) 0.6 g, boric acid (H ₃ BO ₃ , boric acid) 0.1 g, zinc sulfate heptahydrate (ZnSO ₄ 7H ₂ O , zinc sulfate heptahydrate) 0.1g, _{sodium molybdate dihydrate (Na 2} Mo0 ₄ ·2H ₂ 0, sodium molybdate dihydrate) 0.1g, copper sulfate heptahydrate (CuS0 ₄ ·5H ₂ O, copper(I) sulfate pentahydrate) 0.1g , Sodium edetate (EDTA ferric sodium salt trihydrate) 0.5g _{, 3-indolebutyric acid (IBA (C 12} H ₁₃ NO ₂ ), 3-indolebutyric acid) 0.1 g, potassium hydroxide (KOH) 0.1 g, inositol (inositol) 1.3 g, pyridoxine hydrochloride (C ₈ H ₁₁ NO ₃ HCl, pyridoxine hydrochloride) 0.1 g and thiamine hydrochloride 0.1 g, zinc (Zn) 5 g and calcium (Ca) 5 g were added to purified water to obtain a total volume. A liquid medium was prepared to be 12 liters.

Preparation Example 4-No containing monobasic potassium phosphate

Ammonium nitrate (NH ₄ NO ₃ , ammonium nitrate) 5 g, _{calcium nitrate tetrahydrate ([CaNO 3} ] ₂ ·4H ₂ 0, calcium nitrate tetrahydrate) 6.3 g _{, potassium sulfate (K 2} SO ₄ , potassium sulfate) 12.5 g, sulfuric acid Magnesium heptahydrate (MgSO ₄ 7H ₂ O, magnesium sulfate heptahydrate) 1.9g, anhydrous calcium chloride (CaCl ₂ , calcium chloride anhydrous) 0.6g, boric acid (H ₃ BO ₃ , boric acid) 0.1g, zinc sulfate heptahydrate (ZnSO ₄ 7H ₂ O, zinc sulfate heptahydrate) 0.1 g, _{sodium molybdate dihydrate (Na 2} Mo0 ₄ 2H ₂ 0, sodium molybdate dihydrate) 0.1 g, copper sulfate (CuS0 ₄ 5H ₂ O, copper(I) sulfate pentahydrate) 0.1g, sodium edetate ferric (EDTA ferric sodium salt trihydrate) 0.5g _{, 3-indolebutyric acid (IBA(C 12} H ₁₃ NO ₂ ), 3-indolebutyric acid) 0.1g, potassium hydroxide (KOH ) 0.1 g, inositol 1.3 g, pyridoxine hydrochloride (C ₈ H ₁₁ NO ₃ HCl, pyridoxine hydrochloride) 0.1 g and thiamine hydrochloride 0.1 g, zinc (Zn) 5 g and calcium (Ca) 5 g in purified water A liquid medium was prepared so that the total volume was 12 liters.

Preparation Example 5-Without potassium sulfate

Ammonium nitrate (NH ₄ NO ₃ , ammonium nitrate) 5g, _{calcium nitrate tetrahydrate ([CaNO 3} ] ₂ ·4H ₂ 0, calcium nitrate tetrahydrate) 6.3g _{, potassium phosphate (KH 2} PO ₄ , potassium phosphate monobasic) 2.1 g, magnesium sulfate heptahydrate (MgSO ₄ 7H ₂ O, magnesium sulfate heptahydrate) 1.9 g, anhydrous calcium chloride (CaCl ₂ , calcium chloride anhydrous) 0.6 g, boric acid (H ₃ BO ₃ , boric acid) 0.1 g, zinc sulfate 7 Hydrate (ZnSO ₄ ·7H ₂ O, zinc sulfate heptahydrate) 0.1g _{, sodium molybdate dihydrate (Na 2} Mo0 ₄ ·2H ₂ 0, sodium molybdate dihydrate) 0.1g, copper sulfate (CuS0 ₄ ·5H ₂ O, copper (I) sulfate pentahydrate) 0.1 g, sodium _{edetate ferric acid (EDTA ferric sodium salt trihydrate) 0.5 g, 3-indolebutyric acid (IBA (C 12} H ₁₃ NO ₂ ), 3-indolebutyric acid) 0.1 g, hydroxide Potassium (KOH) 0.1 g, inositol 1.3 g, pyridoxine hydrochloride (C ₈ H ₁₁ NO ₃ HCl, pyridoxine hydrochloride) 0.1 g and thiamine hydrochloride 0.1 g, zinc (Zn) 5 g and calcium (Ca) A liquid medium was prepared by putting 5 g in purified water so that the total volume was 12 liters.

Preparation Example 6-Without magnesium sulfate heptahydrate

Ammonium nitrate (NH ₄ NO ₃ , ammonium nitrate) 5g, _{calcium nitrate tetrahydrate ([CaNO 3} ] ₂ ·4H ₂ 0, calcium nitrate tetrahydrate) 6.3g _{, potassium phosphate (KH 2} PO ₄ , potassium phosphate monobasic) 2.1 g, potassium sulfate (K ₂ SO ₄ , potassium sulfate) 12.5 g, anhydrous calcium chloride (CaCl ₂ , calcium chloride anhydrous) 0.6 g, boric acid (H ₃ BO ₃ , boric acid) 0.1 g, zinc sulfate heptahydrate (ZnSO ₄ · 7H ₂ O, zinc sulfate heptahydrate) 0.1 g, _{sodium molybdate dihydrate (Na 2} Mo0 ₄ 2H ₂ 0, sodium molybdate dihydrate) 0.1 g, copper sulfate heptahydrate (CuS0 ₄ 5H ₂ O, copper(I) sulfate pentahydrate ) 0.1g, EDTA ferric sodium salt trihydrate 0.5g _{, 3-indolebutyric acid (IBA(C 12} H ₁₃ NO ₂ ), 3-indolebutyric acid) 0.1g, potassium hydroxide (KOH) 0.1 g, 1.3 g of inositol _{, 0.1 g of pyridoxine hydrochloride (C 8} H ₁₁ NO ₃ HCl, pyridoxine hydrochloride) and 0.1 g of thiamine hydrochloride, 5 g of zinc (Zn) and 5 g of calcium (Ca) were added to purified water. A liquid medium was prepared so that the total volume was 12 liters.

Preparation Example 7-Sodium ferric edetate (EDTA) not included

Ammonium nitrate (NH ₄ NO ₃ , ammonium nitrate) 5g, _{calcium nitrate tetrahydrate ([CaNO 3} ] ₂ ·4H ₂ 0, calcium nitrate tetrahydrate) 6.3g _{, potassium phosphate (KH 2} PO ₄ , potassium phosphate monobasic) 2.1 g, potassium sulfate (K ₂ SO ₄ , potassium sulfate) 12.5 g, magnesium sulfate heptahydrate (MgSO ₄ 7H ₂ O, magnesium sulfate heptahydrate) 1.9 g, anhydrous calcium chloride (CaCl ₂ , calcium chloride anhydrous) 0.6 g, boric acid ( H ₃ BO ₃ , boric acid) 0.1 g, zinc sulfate heptahydrate (ZnSO ₄ 7H ₂ O, zinc sulfate heptahydrate) 0.1 g _{, sodium molybdate dihydrate (Na 2} Mo0 ₄ 2H ₂ 0, sodium molybdate dihydrate) 0.1 g, copper _{sulfate pentahydrate (CuS0 4} 5H ₂ O, copper(I) sulfate pentahydrate) 0.1 g _{, 3-indolebutyric acid (IBA (C 12} H ₁₃ NO ₂ ), 3-indolebutyric acid) 0.1 g, potassium hydroxide ( KOH) 0.1 g, inositol 1.3 g, pyridoxine hydrochloride (C ₈ H ₁₁ NO ₃ HCl, pyridoxine hydrochloride) 0.1 g and thiamine hydrochloride 0.1 g, zinc (Zn) 5 g and calcium (Ca) 5 g A liquid medium was prepared so that the total volume was 12 liters by putting it in purified water.

Preparation Example 8-Without potassium hydroxide

Ammonium nitrate (NH ₄ NO ₃ , ammonium nitrate) 5g, _{calcium nitrate tetrahydrate ([CaNO 3} ] ₂ ·4H ₂ 0, calcium nitrate tetrahydrate) 6.3g _{, potassium phosphate (KH 2} PO ₄ , potassium phosphate monobasic) 2.1 g, potassium sulfate (K ₂ SO ₄ , potassium sulfate) 12.5 g, magnesium sulfate heptahydrate (MgSO ₄ 7H ₂ O, magnesium sulfate heptahydrate) 1.9 g, anhydrous calcium chloride (CaCl ₂ , calcium chloride anhydrous) 0.6 g, boric acid ( H ₃ BO ₃ , boric acid) 0.1 g, zinc sulfate heptahydrate (ZnSO ₄ 7H ₂ O, zinc sulfate heptahydrate) 0.1 g _{, sodium molybdate dihydrate (Na 2} Mo0 ₄ 2H ₂ 0, sodium molybdate dihydrate) 0.1 g, copper _{sulfate pentahydrate (CuS0 4} 5H ₂ O, copper(I) sulfate pentahydrate) 0.1 g, sodium edetate ferric sodium salt trihydrate 0.5 g, 3-indolebutyric acid (IBA(C ₁₂ H ₁₃ NO ₂ ), 3-indolebutyric acid) 0.1 g, inositol 1.3 g, pyridoxine hydrochloride (C ₈ H ₁₁ NO ₃ HCl, pyridoxine hydrochloride) 0.1 g and thiamine hydrochloride 0.1 g, zinc (Zn) 5g and 5g of calcium (Ca) were added to purified water to prepare a liquid medium so that the total volume was 12 liters.

[Example 1]-Using the liquid medium of Preparation Example 1

10 g of natural wild ginseng was cut into a size of 1 to 2 mm to prepare a piece of natural wild ginseng. The prepared wild ginseng pieces were inoculated into the wild ginseng culture medium prepared according to Preparation Example 1, and cultured at 25° C. for 15 days to grow into wild ginseng cell mass. Thereafter, the grown wild ginseng cell mass was collected and divided into a culture container containing the wild ginseng culture medium prepared according to Preparation Example 1, and further grown at 25° C. for 30 days to induce adventitious roots. Next, the adventitious roots formed in this way were harvested, and among them, strong and straight roots were selected, which was again divided into a culture container containing the wild ginseng culture medium prepared according to Preparation Example 1, and contained therein for 40 days at 25°C. It was grown to obtain a number of wild ginseng fine roots. Next, the obtained wild ginseng fine roots were put into the bioreactor containing the wild ginseng culture medium prepared according to Preparation Example 1, and the third culture was performed at 25°C for 15 days, and the wild ginseng fine roots grown in the bioreactor were harvested and prepared. Into a 13-ton large-scale culture tank containing the culture medium for wild ginseng culture prepared according to Example 1, the fourth culture was performed at 25°C for 15 days. Next, the wild ginseng after the fourth culture was obtained, washed with purified water, and dried to obtain cultured wild ginseng.

[Comparative Example 1]-Using the liquid medium of Preparation Example 2

10 g of natural wild ginseng was cut into a size of 1 to 2 mm to prepare a piece of natural wild ginseng. The prepared wild ginseng pieces were inoculated into the wild ginseng culture medium prepared according to Preparation Example 2, and cultured at 25° C. for 15 days to grow into wild ginseng cell mass. Thereafter, the grown wild ginseng cell mass was collected and divided into a culture container containing the wild ginseng culture medium prepared according to Preparation Example 2, and further grown at 25° C. for 30 days to induce adventitious roots. Next, the adventitious roots formed in this way were harvested, and among them, strong and straight roots were selected, and they were divided into a culture container containing the culture medium for wild ginseng culture prepared according to Preparation Example 2, and further contained therein for 40 days at 25°C. It was grown to obtain a number of wild ginseng fine roots. Next, the obtained wild ginseng fine roots were again put into the bioreactor containing the wild ginseng culture medium prepared according to Preparation Example 2, and the third culture was performed at 25°C for 15 days, and the wild ginseng fine roots grown in the bioreactor were harvested and prepared. Into a 13-ton large-scale culture tank containing the culture medium for wild ginseng culture prepared according to Example 2, the fourth culture was performed at 25°C for 15 days. Next, the wild ginseng after the fourth culture was obtained, washed with purified water, and dried to obtain cultured wild ginseng.

[Comparative Example 2]-Using the liquid medium of Preparation Example 3

10 g of natural wild ginseng was cut into a size of 1 to 2 mm to prepare a piece of natural wild ginseng. The prepared wild ginseng pieces were inoculated into the wild ginseng culture medium prepared according to Preparation Example 3, and cultured at 25° C. for 15 days to grow into wild ginseng cell mass. Then, the grown wild ginseng cell mass was collected and divided into a culture container containing the wild ginseng culture medium prepared according to Preparation Example 3, and further grown at 25° C. for 30 days to induce adventitious roots. Next, the adventitious roots formed in this way were harvested, and among them, strong and straight roots were selected, and they were divided into a culture vessel containing the culture medium for wild ginseng culture prepared according to Preparation Example 3, and contained therein for 40 days at 25°C. It was grown to obtain a number of wild ginseng fine roots. Next, the obtained wild ginseng fine roots were again put into the bioreactor containing the wild ginseng culture medium prepared according to Preparation Example 3, and the third culture was performed at 25°C for 15 days, and the wild ginseng fine roots grown in the bioreactor were harvested and prepared. Into a 13-ton large-sized culture tank containing the wild ginseng culture medium prepared according to Example 3, and performing a fourth culture process at 25°C for 15 days. Next, the wild ginseng after the fourth culture was obtained, washed with purified water, and dried to obtain cultured wild ginseng.

[Comparative Example 3]-Using the liquid medium of Preparation Example 4

10 g of natural wild ginseng was cut into a size of 1 to 2 mm to prepare a piece of natural wild ginseng. The prepared wild ginseng pieces were inoculated into the wild ginseng culture medium prepared according to Preparation Example 4 and cultured at 25° C. for 15 days to grow into wild ginseng cell mass. Thereafter, the grown wild ginseng cell mass was collected and divided into a culture container containing the wild ginseng culture medium prepared according to Preparation Example 4, and then further grown at 25° C. for 30 days to induce adventitious roots. Next, the adventitious roots formed in this way were harvested, and among them, strong and straight roots were selected, which was again divided into a culture container containing the wild ginseng culture medium prepared according to Preparation Example 4, and further contained therein for 40 days at 25°C. It was grown to obtain a number of wild ginseng fine roots. Next, the obtained wild ginseng fine roots were put into a bioreactor containing the wild ginseng culture medium prepared according to Preparation Example 4, and the third culture was performed at 25°C for 15 days, and the wild ginseng fine roots grown in the bioreactor were harvested and prepared. Into a 13-ton large-sized culture tank containing the culture medium for wild ginseng culture prepared according to Example 4, the fourth culture was performed at 25°C for 15 days. Next, the wild ginseng after the fourth culture was obtained, washed with purified water, and dried to obtain cultured wild ginseng.

[Comparative Example 4]-Using the liquid medium of Preparation Example 5

10 g of natural wild ginseng was cut into a size of 1 to 2 mm to prepare a piece of natural wild ginseng. The prepared wild ginseng pieces were inoculated into the wild ginseng culture medium prepared according to Preparation Example 5, and cultured at 25° C. for 15 days to grow into wild ginseng cell mass. Thereafter, the grown wild ginseng cell mass was collected and divided into a culture container containing the wild ginseng culture medium prepared according to Preparation Example 5, and further grown at 25° C. for 30 days to induce adventitious roots. Next, the adventitious roots formed in this way were harvested, and among them, strong and straight roots were selected, and they were divided into a culture vessel containing the culture medium for wild ginseng culture prepared according to Preparation Example 5, and contained therein for 40 days at 25°C. Grown to obtain a number of wild ginseng fine roots. Next, the obtained wild ginseng fine roots were again put into the bioreactor containing the wild ginseng culture medium prepared according to Preparation Example 5, and the third culture was performed at 25°C for 15 days, and the wild ginseng fine roots grown in the bioreactor were harvested. Into a 13-ton large-sized culture tank containing the culture medium for wild ginseng culture prepared according to Example 5, the fourth culture was performed at 25°C for 15 days. Next, the wild ginseng after the fourth culture was obtained, washed with purified water, and dried to obtain cultured wild ginseng.

[Comparative Example 5]-Using the liquid medium of Preparation Example 6

10 g of natural wild ginseng was cut into a size of 1 to 2 mm to prepare a piece of natural wild ginseng. The prepared wild ginseng pieces were inoculated into the wild ginseng culture medium prepared according to Preparation Example 6, and cultured at 25° C. for 15 days to grow into wild ginseng cell mass. Thereafter, the grown wild ginseng cell mass was collected and divided into a culture container containing the wild ginseng culture medium prepared according to Preparation Example 6, and further grown at 25° C. for 30 days to induce adventitious roots. Next, the adventitious roots formed in this way were harvested, and among them, strong and straight roots were selected, and they were divided into a culture container containing the culture medium for wild ginseng culture prepared according to Preparation Example 6, and contained therein for 40 days at 25°C. It was grown to obtain a number of wild ginseng fine roots. Next, the obtained wild ginseng fine roots were put into a bioreactor containing the wild ginseng culture medium prepared according to Preparation Example 6, and the third culture was performed at 25°C for 15 days, and the wild ginseng fine roots grown in the bioreactor were harvested and prepared. Into a 13-ton large-sized culture tank containing the wild ginseng culture medium prepared according to Example 6, and performing a fourth culture process at 25°C for 15 days. Next, the wild ginseng after the fourth culture was obtained, washed with purified water, and dried to obtain cultured wild ginseng.

[Comparative Example 6]-Using the liquid medium of Preparation Example 7

10 g of natural wild ginseng was cut into a size of 1 to 2 mm to prepare a piece of natural wild ginseng. The prepared wild ginseng pieces were inoculated into the wild ginseng culture medium prepared according to Preparation Example 7 and cultured at 25° C. for 15 days to grow into wild ginseng cell mass. Thereafter, the grown wild ginseng cell mass was collected and divided into a culture container containing the wild ginseng culture medium prepared according to Preparation Example 7, and then further grown at 25° C. for 30 days to induce adventitious roots. Next, the adventitious roots formed in this way were harvested, and among them, strong and straight roots were selected, which was again divided into a culture container containing the wild ginseng culture medium prepared according to Preparation Example 7 and contained therein for 40 days at 25°C. It was grown to obtain a number of wild ginseng fine roots. Next, the obtained wild ginseng fine roots were again put into the bioreactor containing the wild ginseng culture medium prepared according to Preparation Example 7 and the third culture was performed at 25°C for 15 days, and the wild ginseng fine roots grown in the bioreactor were harvested and prepared. Into a 13-ton large-sized culture tank containing the culture medium for wild ginseng culture prepared according to Example 7 and performing the fourth culture process at 25°C for 15 days. Next, the wild ginseng after the fourth culture was obtained, washed with purified water, and dried to obtain cultured wild ginseng.

[Comparative Example 7]-Using the liquid medium of Preparation Example 8

10 g of natural wild ginseng was cut into a size of 1 to 2 mm to prepare a piece of natural wild ginseng. The prepared wild ginseng pieces were inoculated into the wild ginseng culture medium prepared according to Preparation Example 8, and cultured at 25° C. for 15 days to grow into wild ginseng cell mass. Thereafter, the grown wild ginseng cell mass was collected and divided into a culture container containing the wild ginseng culture medium prepared according to Preparation Example 8, and further grown at 25° C. for 30 days to induce adventitious roots. Next, the adventitious roots formed in this way were harvested, and among them, strong and straight roots were selected, which was again divided into a culture container containing wild ginseng culture medium prepared according to Preparation Example 8, and further contained therein for 40 days at 25°C. It was grown to obtain a number of wild ginseng fine roots. Next, the obtained wild ginseng fine roots were put into the bioreactor containing the wild ginseng culture medium prepared according to Preparation Example 8, and the third culture was performed at 25°C for 15 days, and the wild ginseng fine roots grown in the bioreactor were harvested and prepared. Into a 13-ton large-sized culture tank containing the culture medium for wild ginseng culture prepared according to Example 8, the fourth culture was performed at 25°C for 15 days. Next, the wild ginseng after the fourth culture was obtained, washed with purified water, and dried to obtain cultured wild ginseng.

[Experiment 1]

The contents of zinc (Zn) and calcium (Ca) contained per 1 g of cultured wild ginseng were measured using the cultured wild ginseng prepared through Example 1 and Comparative Examples 1 to 7, and the results are shown in Table 1 below.

division Zinc (Zn) content Calcium (Ca) content Example 1 0.101mg 0.092mg Comparative Example 1 0.021mg 0.012mg Comparative Example 2 0.016mg 0.009mg Comparative Example 3 0.023mg 0.011mg Comparative Example 4 0.014mg 0.015mg Comparative Example 5 0.018mg 0.009mg Comparative Example 6 0.021mg 0.008mg Comparative Example 7 0.019mg 0.007mg

Looking at the results of Table 1, it can be seen that in the case of the wild ginseng cultured root according to Example 1, the content of both zinc (Zn) and calcium (Ca) is significantly higher than that of Comparative Examples 1 to 7.

[Experiment 2]

The total amount of cultured wild ginseng prepared through Example 1 and Comparative Examples 1 to 7 was measured, and the results are shown in Table 2 below.

division Total amount of cultured wild ginseng (kg) Example 1 15.5 Comparative Example 1 6.3 Comparative Example 2 4.5 Comparative Example 3 5.2 Comparative Example 4 3.8 Comparative Example 5 4.7 Comparative Example 6 4.9 Comparative Example 7 5.8

Looking at the results of Table 2, in the case of the wild ginseng cultured root according to Example 1, it can be seen that the yield of cultured wild ginseng is remarkably superior compared to Comparative Examples 1 to 7.

On the other hand, Figure 3 is a comparison of the electrophoresis photos of natural wild ginseng (a) and cultured wild ginseng (b) prepared according to Preparation Example 1, comparing the electrophoresis photo of Figure 3, cultured wild ginseng prepared according to the present invention In the case of, it can be confirmed that the components identified through the moving band of natural wild ginseng and electrophoresis are almost the same.

4 and 5 illustrate the steps of obtaining wild ginseng fine roots, and FIG. 6 shows a test report that can confirm that the cultured wild ginseng prepared according to the present invention does not contain any residual pesticides.

Claims (4)

Preparing a piece of natural wild ginseng by cutting the natural wild ginseng (S100);
Inoculating the natural wild ginseng fragments in a wild ginseng culture medium and first culturing them to grow wild ginseng cell masses (S200);
Further growing the wild ginseng cell mass to induce adventitious muscles (S300);
Selecting a root having excellent traits from among the adventitious roots and secondary culturing the roots to obtain wild ginseng fine roots (S400);
Putting the wild ginseng fine roots into a bioreactor and performing a third culture (S500); And
Including; putting wild ginseng fine roots grown in the bioreactor into a large culture tank and culturing the fourth (S600); and
Each of the first to fourth culture
Ammonium nitrate (NH ₄ NO ₃ , ammonium nitrate), _{calcium nitrate tetrahydrate ([CaNO 3} ] ₂ ·4H ₂ 0, calcium nitrate tetrahydrate) _{, potassium phosphate (KH 2} PO ₄ , potassium phosphate monobasic), potassium sulfate ( K ₂ SO ₄ , potassium sulfate), magnesium sulfate heptahydrate (MgSO ₄ 7H ₂ O, magnesium sulfate heptahydrate) _{, calcium chloride anhydrous (CaCl 2} , calcium chloride anhydrous), boric acid (H ₃ BO ₃ , boric acid), zinc sulfate Heptahydrate (ZnSO ₄ 7H ₂ O, zinc sulfate heptahydrate) _{, sodium molybdate dihydrate (Na 2} Mo0 ₄ 2H ₂ 0, sodium molybdate dihydrate), copper sulfate (CuS0 ₄ 5H ₂ O, copper(I) sulfate pentahydrate), sodium ferric sodium salt trihydrate (EDTA ferric sodium salt trihydrate _{), 3-indolebutyric acid (IBA(C 12} H ₁₃ NO ₂ ), 3-indolebutyric acid), potassium hydroxide (KOH), inositol , Pyridoxine hydrochloride (C ₈ H ₁₁ NO ₃ HCl, pyridoxine hydrochloride), thiamine hydrochloride, zinc (Zn) and calcium (Ca). Cultured root manufacturing method. The method according to claim 1,
Harvesting the wild ginseng that has been cultivated in a large culture tank and washing with purified water (S700); And
Drying the washed wild ginseng (S800); wild ginseng cultured root production method comprising a further. delete The method according to claim 1,
The liquid medium for cultivating wild ginseng is
Per 12 liters of total liquid medium
Ammonium nitrate (NH ₄ NO ₃ , ammonium nitrate) 3 to 7 g _{, calcium nitrate tetrahydrate ([CaNO 3} ] ₂ ·4H ₂ 0, calcium nitrate tetrahydrate) 4 to 7 g _{, potassium phosphate (KH 2} PO ₄ , potassium phosphate) monobasic) 1 to 3 g, potassium sulfate (K ₂ SO ₄ , potassium sulfate) 10 to 15 g, magnesium sulfate heptahydrate (MgSO ₄ 7H ₂ O, magnesium sulfate heptahydrate) 1 to 3 g, anhydrous calcium chloride (CaCl ₂ , calcium chloride anhydrous ) 0.1 to 2 g, boric acid (H ₃ BO ₃ , boric acid) 0.05 to 0.5 g, zinc sulfate heptahydrate (ZnSO ₄ 7H ₂ O, zinc sulfate heptahydrate) 0.05 to 0.5 g, sodium molybdate dihydrate (Na ₂ Mo0 ₄ ·2H ₂ 0, sodium molybdate dihydrate) 0.05 to 0.5 g, copper sulfate (CuS0 ₄ ·5H ₂ O, copper(I) sulfate pentahydrate) 0.05 to 0.5 g, sodium edetate ferric sodium salt trihydrate ) 0.1 to 2 g, _{3-indolebutyric acid (IBA (C 12} H ₁₃ NO ₂ ), 3-indolebutyric acid) 0.05 to 0.5 g, potassium hydroxide (KOH) 0.05 to 0.5 g, inositol 0.5 to 3 g, pyridoxine Hydrochloride (C ₈ H ₁₁ NO ₃ HCl, pyridoxine hydrochloride) 0.05 to 0.5 g and thiamine hydrochloride (thiamine hydrochloride) 0.05 to 0.5 g, characterized in that it comprises a wild ginseng cultured root production method.

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