KR102224350B1 - Agent for reducing skin irritation and cosmetic composition for skin whitening containing the same - Google Patents

Abstract

The present invention relates to a skin irritation emollient containing the green tea tree root extract as an active ingredient and a cosmetic composition for skin whitening comprising the same.

Description

Skin irritation emollient and skin whitening cosmetic composition containing the same {AGENT FOR REDUCING SKIN IRRITATION AND COSMETIC COMPOSITION FOR SKIN WHITENING CONTAINING CONTAINING THE SAME}

The present invention relates to a skin irritation reliever and a cosmetic composition for whitening comprising the same.

Women want to have a young and white skin color as a standard of beauty, and in response to these demands, interest in whitening functional cosmetics is also increasing in the cosmetic industry.

Recently, as a number of mechanisms of melanin pigmentation have been revealed, various types of whitening agents have been developed. The color of the skin is determined by the amount and distribution of skin pigments such as melanin, and when the skin is exposed to ultraviolet rays, tyrosine in the melanosomes of melanocytes is converted by an enzyme called tyrosinase. It is produced by oxidation. The melanin produced in this way is transferred to the keratinocytes through dendrite of melanocytes. Melanin plays an important role in preventing skin damage from UV rays.However, when the amount of melanin produced is increased due to certain factors, a large amount of melanin produced by this is transferred to keratinocytes, thereby accumulating melanin in the skin's epidermis and pigmentation such as spots Causes.

Niacinamide is a physiologically active amide of vitamin B3, also known as pyridine 3 carboxylic acid, and inhibits the movement of melanosomes, thereby inhibiting the movement of synthesized melanin to keratinocytes. Accordingly, skin pigmentation is reduced to increase the whitening effect.

However, since niacinamide having a whitening function causes skin irritation when applied to the skin, there is a limitation that it cannot be contained in a certain concentration or more when used as a cosmetic ingredient.

In general, when external stimulation is given, Keratinocoyte or Langerhan's cells make and release various cytokines. 8: IL-8) and other cytokines are essentially released.

Hydrocortisone, known to be effective in relieving skin inflammation and skin irritation, is a steroid and causes side effects such as skin atrophy and vasodilation.

Therefore, while containing niacinamide at a concentration that has a sufficient skin whitening effect or higher, it is possible to reduce or inhibit the release of cytokines such as IL-8 without containing steroids such as hydrocortisone, thereby reducing skin irritation caused by niacinamide. There is a need to develop cosmetic materials that can be used.

Korean Patent Publication No. 10-2009-0091511 (2009.08.28)

An object of the present invention is to provide a skin irritation emollient having an effect of reducing irritation when applied to the skin, and a cosmetic composition including the same, which has a skin whitening effect and has a low irritation when applied to skin.

The present invention provides a skin irritation reliever containing a green tea tree root extract as an active ingredient.

In addition, the present invention provides a cosmetic composition for skin whitening containing a green tea tree root extract and niacinamide as an active ingredient.

The skin irritation emollient according to the present invention has the effect of suppressing or reducing skin irritation caused by niacinamide, and the cosmetic composition for skin whitening according to the present invention contains niacinamide to provide excellent skin whitening effect and skin irritation caused by niacinamide. This is low.

1 shows the analysis results of NMR and FAB/MS using the green tea root extract powder of Example 1 as a sample, where the horizontal axis represents the amount of charge (unit: m/z), and the vertical axis represents the relative intensity (relatative intensity, based on 100). to be.

In the present specification, the term "skin" refers to a tissue covering the body surface of an animal, and is the broadest concept including not only the tissue covering the body surface such as a face or body, but also the scalp and hair. In addition, in the present specification, "sensitive skin" has low resistance to external stimuli and may be defined as skin that is susceptible to adverse reactions such as itching, itching, burning, burning, redness, dryness, and inflammation due to external stimuli. , The cause of such sensitive skin may be congenital skin characteristics, family history, medical history, misuse of cosmetics, etc., life environment or stress. In the present specification, "extract" is a broad concept including all substances obtained by extracting a component of a natural substance, regardless of an extraction method, an extraction solvent, an extracted component, or a form of an extract.

In the present specification, green tea tree refers to Camellia Sinensis of the tea tree family.

The present invention provides a skin irritation reliever containing a green tea tree root extract as an active ingredient.

In one embodiment of the present invention, the Green Tea Root Extract is a step of washing green tea tree roots, pulverizing, extracting with a solvent, filtering, concentrating, and drying It is produced by carrying out.

The step of extracting with the solvent may be extraction with a 70% aqueous ethanol solution.

The filtering may be filtering with a filter having a diameter of 5 μm, 1 μm, 0.5 μm, and/or 0.2 μm of filter pores.

In one embodiment of the present invention, the green tea tree root extract contains green tea ponin.

The green tea phonin may be represented by the following Formula 1.

[Formula 1]

In one embodiment of the present invention, the skin irritation is for alleviating skin irritation caused by niacinamide. More specifically, the skin irritation may be skin irritation caused by niacinamide when a skin external preparation including niacinamide is applied to the skin.

The skin irritation emollient contains the green tea tree root extract as an active ingredient to inhibit interleukin-8 (IL-8) secreted into the skin when the niacinamide component comes into contact with the skin. Allows amide ingredients to be applied to the skin.

In one embodiment of the present invention, the content of the green tea tree root extract is 3000 ppm or less when 6.25 ppm or more is applied with respect to the total weight of the skin irritation reliever. If the content of the green tea tree root extract is 6.25 ppm or more with respect to the total weight of the skin irritation reliever, when niacinamide is applied to the skin, interleukin-8 secreted into the skin is suppressed, thereby reducing irritation caused by niacinamide. have. If more than 3000 ppm is used, the effect of alleviating irritation is no longer improved.

The present invention provides a cosmetic composition for whitening containing green tea tree root extract and niacinamide as an active ingredient.

The cosmetic composition for whitening according to the present invention contains a green tea tree root extract that reduces skin irritation caused by niacinamide, so that the cosmetic composition may contain a high concentration of niacinamide compared to a cosmetic composition containing niacinamide alone. When applied to the skin irritation can be appropriately suppressed, and for this reason, it can contain a high concentration of niacinamide, so when the cosmetic composition is applied to the skin, the melanin synthesized in the skin is strongly inhibited from moving to keratinocytes. It can further increase the whitening effect by reducing skin pigmentation.

In one embodiment of the present invention, the content of the green tea tree root extract is 6.25 ppm or more and 3000 ppm or less based on the total weight of the skin whitening cosmetic composition. If the content of the green tea tree root extract is 6.25 ppm or more with respect to the total weight of the skin whitening cosmetic composition, the effect of suppressing interleukin-8 secreted to the skin when niacinamide is applied to the skin to relieve irritation caused by niacinamide And, when it exceeds 3000 ppm, the irritation relief effect is no longer improved.

In one embodiment of the present invention, the weight ratio of the green tea tree root extract: the niacinamide is 1:3000 to 1:6. If the weight ratio of the green tea tree root extract: the niacinamide is 1:3000 or more, it is effective, and if it exceeds 1:6, the stimulation alleviating effect is no longer enhanced.

The cosmetic composition for skin whitening according to the present invention may be formulated containing a medium or base that is acceptable cosmetically or dermatologically. These are all formulations suitable for topical application, such as solutions, gels, solids, paste anhydrous products, emulsions obtained by dispersing the oil phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or, ionic (liposomes) and It may be provided in the form of a nonionic vesicle dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. The cosmetic may be prepared according to a conventional method in the art.

In addition, the cosmetic is a fatty substance, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic or nonionic emulsifier. , Fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredient commonly used in cosmetics, such as cosmetic or It may contain adjuvants commonly used in the field of dermatology. The adjuvant is introduced in an amount generally used in the field of cosmetology or dermatology.

Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to test examples and examples. However, these Test Examples and Experimental Examples are provided for illustrative purposes only to aid understanding of the present invention, and the scope and scope of the present invention are not limited by the following Examples.

<Example 1>

After washing green tea tree roots with water, it was pulverized to a certain size. The pulverized green tea tree roots were put in a 70% ethanol aqueous solution (1:8) and extraction was performed at a temperature of 60° C. for 24 hours. The green tea tree root extract was sequentially filtered through filters having filter pore diameters of 5 µm, 1 µm, 0.5 µm, and 0.2 µm. The filtered green tea tree root extract was removed from the solvent at a temperature of 60° C. and then vacuum-dried at 70° C. for 24 hours to prepare a green tea tree root extract powder.

Using the green tea root extract powder as a sample, using NMR (Varian 500 MHz, Inova 500 Spectrometer, Varian Inc., USA) and FAB/MS (JMS 700, Jeol Ltd., Japan), components contained in the green tea root extract It was confirmed that it was green tea ponin, and the results are as shown in FIG. 1.

<Comparative Example 1>

The dried green tea leaves were put in a 70% ethanol aqueous solution (1:8) and extraction was performed at a temperature of 60° C. for 24 hours. The green tea tree root extract was sequentially filtered through filters having filter pore diameters of 5 µm, 1 µm, 0.5 µm, and 0.2 µm. The filtered green tea tree root extract was vacuum-dried at 70° C. for 24 hours after removing the solvent at a temperature of 60° C. to prepare a green tea leaf extract powder.

<Test Example 1> Evaluation of IL-8 release inhibition rate against niacinamide stimulation.

Test cells, keratinocytes (cell line name: HaCaT, source: Dr. NE Fusenig, Deutsches Krebsforschungszentrum, Heidelberg, Germany) and 10% fetal bovine serum (Fetal bovine sereum, FBS, source: Lonza, USA) and 1 % Penicillin-Streptomycin (Penicillin-Streptomycin, source: Lonza, USA) containing DMEM (Dulbecco's Modified EagleMedium, source: Lonza, USA) using a medium, 96-well plate (48 well plate) about 2 After dispensing at a density of x 10 ⁴ cells/well, it was incubated for 24 hours under conditions of 37°C and 5% CO _2. After removing the culture solution, it was washed once with 200 µl of phosphate buffered saline (PBS), and 200 µl of DMEM medium containing no fetal calf serum was added and incubated for 6 hours. Thereafter, Example 1 (green tea tree root extract powder), Comparative Example 1 (green tea leaf extract powder) and positive control (hydrocortisone) were treated for 10 minutes at the concentrations mentioned in Table 1 below with DMEM containing 1% fetal calf serum. After that, it was treated with niacinamide at a concentration of 500 ppm and incubated for 16 hours. 50 µl of the supernatant from the cultured solution was taken, and the inhibitory effect on the release of IL-8 was evaluated by ELISA using an IL-8 ELISA kit (obtained from: BD pharmigen, USA). Specifically, a standard curve was drawn based on the absorbance of the standard substance rh IL-8 (from BD pharmigen, USA), and the reaction equation between the absorbance and the standard substance was obtained, and then the absorbance of the treated substance was substituted, and these inflammation mediators IL A secretion amount of -8 was obtained. Thereafter, according to the formula below, the release inhibition rate (%) of IL-8 was calculated, and the results are shown in Table 1 below.

Free inhibition rate (%) = [1-(IL-8 secretion in the group treated with the test concentration-IL-8 secretion in the untreated group) / ((IL-8 secretion in the group treated with 500ppm niacinamide)-( IL-8 secretion of untreated group))] × 100

Test substance Test concentration IL-8 IL-8 secretion
(pg/mL) Glass suppression rate (%) Niacinamide 500 ppm 669.80 0.00 Example 1 (green tea tree root extract powder) 12.5 ppm 439.11 58.37 6.25 ppm 445.05 56.87 Comparative Example 1 (Green Tea Leaf Extract Powder) 12.5 ppm 563.54 26.89 6.25 ppm 580.94 22.48 Positive control
(Hydrocortisone)
Hydrocortisone 50 nM 342.78 82.74 Untreated group - 274.58 100.00

Claims (6)

As a cosmetic composition for alleviating skin irritation reactions containing green tea tree root extract as an active ingredient,
The skin irritation is characterized in that due to niacinamide, a cosmetic composition for relieving skin irritation reaction. The cosmetic composition of claim 1, wherein the green tea tree root extract contains green tea ponin represented by the following formula (1).
[Formula 1]

The cosmetic composition of claim 1, wherein the skin irritation reaction is any one or more of irritation, itching, burning, burning, redness, dryness, and inflammation occurring in sensitive skin. The cosmetic composition according to claim 1, wherein the content of the green tea tree root extract is 6.25 ppm or more and 3000 ppm or less based on the total weight of the composition. delete delete

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