KR102200411B1 - Additive composition for feed - Google Patents

Abstract

The feed additive composition according to the present invention may include red pepper (Capsicum annuum) powder.
The feed additive composition according to the present invention promotes the decomposition of antibiotics and antibacterial agents accumulated in livestock by using vegetable materials, and discharges or reduces accumulated heavy metals.
In addition, the feed additive composition according to the present invention allows the immunity of livestock to be improved, thereby reducing the use of antibiotics and increasing the production of livestock products, and ultimately improving the quality of livestock products.

Description

Additive composition for feed {ADDITIVE COMPOSITION FOR FEED}

The present invention relates to an additive composition for feed. In more detail, the present invention relates to an additive composition for feed that includes a vegetable material, increases the immunity of livestock, and reduces residual heavy metals and antibiotics, thereby improving the quality of meat as well as enhancing the productivity of livestock.

As consumers become more advanced and diversified in their diet, awareness and sales of high-quality meats that are not only healthy but also taste good are increasing. Therefore, it is true that in response to the demands of these consumers, producers are conducting many tests and studies to improve the quality of meat products in various ways. In particular, studies on blended feeds and feed additives are constantly being conducted to produce high-quality meat. For example, various feed-related products such as feed using loess, feed using bamboo vinegar, feed using green tea, and feed using brown rice are being studied. It is commercially available.

That is, in general, livestock such as pigs, cows, and chickens are raised for use as animal food sources for humans. In such livestock breeding, the biggest problem is that immunity to diseases and other environmental factors and physiological activity are lowered due to changes in the breeding environment. This is not a natural habitat, but high-density breeding, increased use of chemical treatment agents for feed ingredients, and increased feeding amount of processed feed are known to be the main causes.

If resistance to diseases of livestock, that is, immunity and physiological activity, is lowered due to the above causes, it leads to stagnation of growth and death.In order to prevent this, if the amount of drugs such as antibiotics is increased, drugs such as antibiotics are accumulated in the body. As a result, problems leading to the problem occur, which has an important effect on the productivity and economics of livestock farms.

Accordingly, feed additives or supplementary feeds refer to additives used to improve the digestibility, physiological activity, growth promotion, and immunity of livestock products by adding them to the blended feed. As the national diet became westernized, the consumption of livestock products increased. Negative views have been raised due to excessive consumption of animal fat. In particular, as cholesterol is pointed out as one of the main causes of arteriosclerosis, it is necessary to improve supplementary feeds that can lower LDL cholesterol in livestock products and enhance HDL cholesterol. In addition, the livestock industry generally uses antibiotics that can promote livestock growth and prevent and treat livestock diseases (Guo, FC et al., 2004, Effect of a Chinese herb medicine formulation, as an alternative for antibiotics, on performance of broilers, Br Poult Sci., 45, 793-797), if antibiotics are frequently used, resistant microorganisms increase and the effectiveness of the antibiotics decreases or remains in livestock products, causing adverse effects on the human body. The use of antibiotics is increasingly being regulated in that they can cause (Chae, MH et al., 2011, Antimicrobial resistance in Campylobacter jejuni and Campylobacter coli isolated from food animals and raw meats in slaughterhouse in Korea during 2010, Kor. J. Vet.Publ.Hilth., 35, 239-245).

Therefore, by using feed additives derived from natural products, functional additives based on various natural materials are used to reduce the amount of antibiotics remaining in livestock products by enhancing the immunity of livestock itself, and to allow the discharge or reduction of heavy metals accumulated in livestock. It is being studied.

KR 10-2017-0132475 A KR 10-2016-0040790 A

It is an object of the present invention to provide a feed additive composition that promotes the decomposition of antibiotics and antimicrobial agents accumulated in livestock using vegetable materials, and allows the discharge or reduction of accumulated heavy metals.

In addition, the feed additive composition according to the present invention is intended to improve the quality of livestock products while reducing the use of antibiotics and increasing the production of livestock products by enabling the immunity of livestock to be improved.

Another object of the present invention is to provide a feed prepared by using the feed additive composition.

In order to achieve the above object, the feed additive composition according to an embodiment of the present invention is to include red pepper (Capsicum annuum) powder.

The chili powder may be chili pepper powder.

The feed additive composition may further include pepper seed powder.

The feed additive composition may be one further comprising a thistle extract.

The feed additive composition may include the peppercorn stem powder, and the pepper seed powder may be contained in an amount of 10 to 800 parts by weight based on 100 parts by weight of the pepper fruit stem powder.

The composition for feed additives may include the pepper fruit stem powder, and 10 to 800 parts by weight of pepper seed powder and 10 to 800 parts by weight of thistle extract based on 100 parts by weight of the pepper fruit stem powder.

Feed according to another embodiment of the present invention may be one containing the composition for feed additives.

Hereinafter, the present invention will be described in more detail.

Feed additive composition according to an embodiment of the present invention is to include a pepper (Capsicum annuum) powder.

The pepper referred to in the present invention includes the fruit of the pepper tree as a commonly used meaning, and is defined as including the pepper fruit flesh, pepper seed, and pepper fruit stem.

When the pepper powder is used as a feed additive, it not only enhances the immune activity of livestock, but also reduces residual antibiotics and heavy metals accumulated in livestock during the breeding process, so that better quality livestock products can be provided. do.

The chili powder may be chili pepper powder.

Compared to the case of using red pepper flesh or whole red pepper, which is commonly used in red pepper cooking, when the above-mentioned pepper fruit sprout powder is used as a feed additive, the active ingredient contained in the red pepper fruit increases the immunity-enhancing effect and accumulates antibiotics. The back decreases. Particularly, when the pepper fruit stem powder is included in the feed additive, the meat quality of livestock products may be improved.

The feed additive composition may further include pepper seed powder.

In other words, when using a mixture of pepper seed powder and pepper seed powder, the immunity activity of livestock raised is significantly higher than that of the powder obtained by crushing the whole pepper or the powder from the pulp of the pepper fruit. And the flavor becomes much superior.

The pepper seeds and pepper fruit stems are by-products of pepper and are generally discarded without being used in the food manufacturing stage, and they are usually discarded or used as compost. However, in the case of the present invention, the pepper by-products discarded can be effectively utilized as a use of the composition for feed additives.

The feed additive composition may be one further comprising a thistle extract.

Thistle (Cirsium japonicum) is also called a thorny plant, and it grows in mountains or fields. On the other hand, the stem stands upright and is 50-100cm high, with white hairs and cobweb-like hairs on the whole. Root leaves of thistle remain until flowering and are larger than stem leaves. Stem leaves are oval shaped like a lanceolate, split like feathers, and wrap around the circle at the bottom, and the split edges are split again, and there are thorns with serrated teeth in the shape of a deep recess. Flowers bloom from June to August and are purple to red. Head flowers hang at the ends of branches and stems. The gun pieces are arranged in 7 or 8 rows, the longer the inside. Tubular hair is 16 to 19 mm long. Soft plants are used as herbs and mature roots are used for medicinal purposes. Fruits are achene and are 3.5 to 4mm long. It is distributed in Korea, Japan, northeastern China and Ussuri. Those with narrow, green, and somewhat more thorns are called narrow leaf thistles, those with more thorns and more thorns are called thorn thistles, and those with white flowers are called white thorn thistles.

When the thistle extract is further included, the productivity of the livestock to be raised may be increased, and a livestock product having a meat quality of organoleptic superior quality may be provided.

In particular, when the thistle extract is mixed with the pepper fruit stem powder and pepper seed powder and used together in the feed additive composition, the amount of residual antibiotics and heavy metals accumulated in livestock products can be reduced by interaction, and the productivity and production of livestock products It can increase the flavor and oily quality of livestock products that have been made, so that it can produce livestock products of excellent quality.

Preferably, the composition for feed additives may be one further comprising a new green (toscano, Braccica oleracea) extract.

The new green is a type of broccoli of the cruciferous family, and is also called Toscano. Broccoli mainly eats flower buds and stalks, while New Green is leaf broccoli that eats leaves. The leaves are blackish green, and the front is convex. It is relatively easy to cultivate due to its strong resistance to cold, moisture, and disease. When the leaves grow to the size of a palm, they are harvested by picking the leaves one by one, but the yield is large due to the rapid growth of the leaves.

When the composition for feed additives further includes a New Green extract, livestock products having excellent texture and flavor can be produced organoleptic, and it is helpful in the growth and development of livestock, thereby helping to increase production. On the other hand, it is possible to produce high-quality livestock products by activating the immune function and reducing the amount of antibiotics accumulated in livestock.

The feed additive composition may include the peppercorn stem powder, and the pepper seed powder may be contained in an amount of 10 to 800 parts by weight based on 100 parts by weight of the pepper fruit stem powder.

In the case of the above range, it may be helpful to increase the production amount and improve the quality of livestock products due to a synergistic effect due to the interaction of each raw material.

The composition for feed additives may include the pepper fruit stem powder, and 10 to 800 parts by weight of pepper seed powder and 10 to 800 parts by weight of thistle extract based on 100 parts by weight of the pepper fruit stem powder.

In the case of the above range, it is possible to reduce antibiotics accumulated in livestock products, and as a livestock product having excellent flavor and texture, it can contribute to an increase in production.

Preferably, the composition for feed additives comprises the peppercorn stem powder, and the pepper seed powder is 100 to 600 parts by weight based on 100 parts by weight of the pepper fruit stem powder; Thistle extract may be included in an amount of 100 to 600 parts by weight and 200 to 400 parts by weight of New Green extract.

In the case of the above range, it is possible to greatly increase the production efficiency of livestock products by increasing the growth of livestock while removing the accumulated antibiotics as a synergistic effect due to the interaction of each powder and extract. In particular, in the case of the above range, the produced livestock product can produce a high-quality product that is sensory and very excellent.

Feed according to another embodiment of the present invention may be one containing the composition for feed additives.

The feed additive composition according to the present invention promotes the decomposition of antibiotics and antimicrobial agents accumulated in livestock by utilizing vegetable materials, and discharges or reduces accumulated heavy metals.

In addition, the feed additive composition according to the present invention allows the immunity of livestock to be improved, thereby reducing the use of antibiotics and increasing the production of livestock products, and ultimately improving the quality of livestock products.

The present invention makes it possible to provide a feed exhibiting the above-described effects through feed prepared using the feed additive composition.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. However, the present invention may be implemented in various different forms and is not limited to the embodiments described herein.

[Production Example: Preparation of feed additive]

1. Preparation of powder

(1) Preparation of red pepper powder

The pepper flesh from which the pepper fruit stem and pepper seeds were removed was dried and dried at 35 to 40° C., and pulverized to 300 mesh or less to prepare pepper flesh powder (A-1).

(2) Preparation of pepper fruit spout powder

The stem of the pepper fruit was dried at 35 to 40°C. The dried fruit stem and pepper seeds were pulverized to less than 300 mesh to prepare pepper fruit stem powder (A-2).

(3) Preparation of pepper seed powder

Pepper seeds were dried at 35 to 40°C. The dried fruit stem and pepper seeds were pulverized to less than 300 mesh to prepare pepper fruit stem powder (A-3).

(4) Mixing of pepper seed fruit stem powder and pepper seed powder

The pepper seed fruit stem powder and the pepper seed powder were mixed to prepare a pepper seed fruit stem and pepper seed mixed powder (A-4).

2. Preparation of extract

(1) Preparation of thistle extract

After selecting and pulverizing thistle (Korean thisle, Cirsium japonicum maachii), hot water extraction for 2 hours or more using purified water of about 8 times the volume (w/v%) of the weight of the pulverized product as a solvent, and 4 times of purified water And second extraction. The extract was filtered through a filter cloth, and the filtrate was concentrated in vacuo, and then freeze-dried to prepare a thistle extract (EXG).

(2) Preparation of New Green Extract (B-2)

After sorting and pulverizing new green (toscano, Braccica oleracea), extracting purified water with a volume (w/v) of about 7 times the weight of the pulverized product as a solvent for about 4 hours by hot water extraction, and extracting 4 times more purified water. And second extraction. The extract was immediately filtered, concentrated in vacuo, and then freeze-dried to prepare a New Green extract (EXT).

3. Preparation of feed additives

A feed additive was prepared by mixing the prepared A-1 to A-4 and EXG, EXT in the same ratio as in [Table 1] below.

S1 S2 S3 A-1 100 - - A-4 - 100 100 EXG 100 100 100 EXT - - 100

(Unit: parts by weight)

[Experimental Example: Evaluation of feed additives]

1. Experiment on the effect of improving the productivity of chickens by using feed additives

The 20-day-old chickens were separately bred for 30 days and fed for 30 days by dividing them into a general feed feeder (control, C) and a feed feeder with feed additives of S1 to S3. Feed additives were added by 5% based on the total weight of feed, and separate breeding was carried out while maintaining indoor temperature of 20 to 25 degrees and humidity of 65 to 70% in a farm of 1.8 X 1.4 m width x length.

As can be seen in the following [Table 2], all 60 experimental heads fed with the feed additive of the present invention S3 survived, and 3 heads of the control group (C) died. The number of mortality in general chicken meat is statistically about 5%. In the case of average body weight, in the case of chickens ingesting feed containing feed additives consisting of the composition of S3, it was confirmed that the weight gain was improved by about 15% compared to the control (C).

Item S1 S2 S3 C Average weight (Kg) 1.59 1.61 1.81 1.58 Number of dead heads (heads) 3 2 0 3

Therefore, it can be seen that the productivity of chickens is greatly increased in the case of the composition of S3.

2. Sensory characteristics of chicken meat produced through feeding feed additives

A sensory test was conducted on 30 people using chicken meat from poultry fed feed containing the feed additive powder of S1 to S3 and chicken meat (control, C) fed feed without feed additive as a sample. I did. Carcasses were cut into dimensions of width X length X height 5.0 cm X 3.0 cm X 1.0 cm, and the cut meat was heated with propane gas behind an iron plate and sufficiently baked for 10 minutes each.

The sensory test was evaluated based on a 7-point grade system (7=I like the best, 4=I don't like it or hate it, 1=I don't like it the most), and 30 adults in their 30s were evaluated by sensory testers. The results are shown in Table 3 below.

Item S1 S2 S3 C Juicy 4.25 5.25 5.53 4.11 year 4.21 4.67 4.90 3.85 Flavor 4.14 5.51 5.76 4.01

(Unit: grade average)

Referring to [Table 3], it can be seen that the overall functionality is improved in the case of S1 to S3. Particularly, in the case of Example S2, it can be seen that when using pepper fruit powder and pepper seed powder, the sensory properties of the produced chicken meat are much higher than when using pepper fruit powder.

On the other hand, in the case of the composition of S3, it can be seen that the functionality is improved evenly in succulent, softness, and flavor, and the functionality is most excellently increased.

3. Examination of the residual amount of antibiotics in chicken meat produced through feed additives

Enroflo, a fluoroquinolone-based synthetic antibacterial agent, was sampled from chicken meat from poultry fed with feed containing the feed additive powder of S1 to S3 and chicken meat from poultry fed with feed without feed additive (control, C). The residual amount of enrofloxacin was checked.

Test operation is a liquid chromatograph measuring instrument according to the Food Code (separate book) 15. Remaining substance test method in food, 3) Synthetic antibacterial agent, (2) fluoroquinolone type [Enrofloxacin] among quantitative test methods It was done using. After finely grinding the chicken meat, take exactly 10 g, add 40 ml of dichloromethane, and homogenize for 2 minutes with a homogenizer. The homogenate is centrifuged at 8,000 rpm for 20 minutes, and a small amount of the liquid in the upper layer of the meat is removed with a Pasteur pipette, and the entire amount of the dichloromethane layer is collected and transferred to a concentrator, and the same operation is performed for the residue. Repeat once more to combine the extract. The collected extract is concentrated under reduced pressure at 35° so that it becomes about 1 ml. To this concentrate, 40 ml of 0.1N hydrochloric acid solution was added and washed in a separatory filter. 40 ml of hexane was added and shaken vigorously for 5 minutes to separate the hexane layer from the 0.1N hydrochloric acid layer. The 0.1N hydrochloric acid layer was transferred to another separating filter, 40 ml of dichloromethane was added, shaken vigorously for 5 minutes, and the layers were separated to obtain a dichloromethane layer. This extraction operation is repeated twice. The extract is dried under reduced pressure at 35°, dissolved by adding 2 ml of the mobile phase, filtered through a 0.45 μm membrane filter, and used as a test solution.

For analysis, column C18 (4.6 mm × 250 mm, 5 μm) or equivalent is used, and the mobile phase is 0.01 M hydroxyl: acetonitrile: methanol (6: 3: 1), flow rate 1.0 ml/min, excitation wavelength 327 nm, measurement wavelength 369 nm Depending on the conditions of, test solution and standard solution are injected into the liquid chromatography. Comparing the retention times of each obtained peak, a calibration curve is drawn with the height or area of the peak to obtain the content of enrofloxacin, respectively.

The results are shown in the following [Table 4]. Referring to [Table 4] below, it was confirmed that S1 had a slight reduction effect compared to control C, and that S2 produced a higher degree of antibiotic reduction effect than this. On the other hand, feeding feed containing the feed additive powder of S3?h The amount of antibiotic residues in chicken meat of poultry was not detected at all in eggs, and a statistically significant decrease was confirmed in broilers compared to the control. I could confirm that.

Item S1 S2 S3 C Enrofloxacin (in broiler) 0.22 0.12 0.02 0.25 Enrofloxacin (in egg) 0.81 0.67 Not detected 0.86

(Unit: mg/kg)

5. Effect of improving pig productivity by feeding feed additives

In order to investigate the productivity (weight gain) effect of the feed additives according to the present invention S1 to S3, 50 piglets were fed with regular feed, respectively, and the feed with feed additive powder was divided into feed groups for 6 months. After free feeding, the average weight was measured.

Average weight of piglets fed freely for 6 months with feed supplemented with 5% of the feed additive powder of Preparation Example 1 based on the total weight of the feed for pig pigs and piglets fed freely for 6 months without feed additive powder (control) , The results of the investigation of the feed demand rate and the number of dead heads are shown in [Table 5].

Item S1 S2 S3 C Average weight (Kg) 9.85 106.4 118.37 99.22 Feed demand rate 2.12 2.55 2.17 3.01 Number of dead heads (heads) One One 0 3

Referring to [Table 5], in the case of S1, no special improvement effect was confirmed compared to the control (C), and in the case of S2, it was confirmed that the average weight and feed requirement rate were increased.

In particular, pigs fed with feed supplemented with S3 feed additives significantly improved their average weight and feed demand compared to pigs in the control group, thereby improving overall pig farming productivity. They survived for 6 months without any mortality, resulting in immunity. Overall improvement was confirmed.

6. Sensory characteristics of pork produced through feeding feed additives

A sensory test was performed on 30 subjects using the pork of pigs fed the feed containing the feed additive powder of S1 to S3 and the pork of pigs fed feed without the feed additive (control) as samples. Carcasses were cut into dimensions of width X length X height 5.0cm X 3.0cm X 1.0cm, and the cut meat was heated with propane gas behind an iron plate and baked sufficiently for each 17 minutes.

The sensory test was evaluated based on a 7-point grade system (7=I like the best, 4=I don't like it or hate it, 1=I don't like it the most), and 30 adults in their 30s were evaluated by sensory testers. The results are shown in the following [Table 6].

Item S1 S2 S3 C Juicy 5.14 5.95 6.53 4.76 year 5.64 5.46 5.87 4.46 Flavor 4.29 5.14 6.34 4.32

(Unit: grade average)

Referring to [Table 6], when S1 was included, the succulent and year were slightly increased, and when S2 was included, compared with the control (C), it was found that the sensory properties were improved in all items of succulent, year and flavor. I can.

In particular, in the case of S3, it can be confirmed that the juiciness, year, and flavor of pork prepared by ingesting it are improved to a superior grade compared to the control.

7. Inspection of the residual amount of antibiotics in pork produced by feeding feed additives

The residual amount of penicillin synthetic antibacterial ampicillin (ampicillin) was determined by using as a sample the pork of pigs fed with feed containing the feed additive powder of S1 to S3 and pork of pigs fed with feed without feed additives (control). Confirmed.

The test operation was conducted according to the method described in the Handbook for Analysis of Hazardous Substances in Livestock Products, and reagents were prepared by using a liquid chromatograph-mass spectrometer LC-MS/MS as a measuring instrument, using all of the standard ampicillin with HPLC grade. The test solution is 2 g of a homogenized sample by preparing 0.05% formic acid in water, 0.05% formic acid in Actonitrile, 0.1M phosphate buffer (pH 4.5), 0.17 M sulphruic acid, 5% sodoium tungstate, 25 mm PBS (pH 9.0). Add 100uL of standard solution with a concentration of 1ug/mL to the spike sample at a concentration of 0.05ug/g.

For analysis, the column XBridge C18 (2.1 mm × 150 mm, 3.5 μm) or equivalent was used, the mobile phase was 0.05% FA in water/0.05% FA in MeCN gradient, and the ionization was ESI (positive), Capiilary (kV): 3 ,00, Exractor (V): 0.3, Source temperature 150 degrees, Desolvation temperature 350 degrees, Gas Flow: Desolvation 650 L/hr, Cone 50 L/hr, Auxilary Gas: Nitrogen Collision Gas: Test solution and standard according to argon conditions The solution is injected into the liquid chromatography. The retention times of each obtained peak are compared, and a calibration curve is drawn with the height or area of the peak, and the ampicillin content is obtained, respectively.

Item S1 S2 S3 C Ampicillin 0.14 0.14 0 0.02 Detection frequency 1 case 1 case 0 cases 4 cases

(Unit: mg/kg)

As a result, it was confirmed that the antibiotic reduction effect of S1 and S2 was confirmed. In particular, the residual amount of antibiotics in pig meat of pigs fed with feed containing the feed additive of S3 was hardly detected, and the detection frequency was 0 cases. Therefore, it can be seen that the composition of S3 can have an excellent effect in removing residual antibiotics in pork.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention defined in the following claims are also present. It belongs to the scope of rights of

Claims (7)

It contains red pepper berry stem powder,
It further contains pepper seed powder, thistle extract and new green extract,
Improve livestock immunity, quality and productivity,
Which can reduce residual heavy metals and antibiotics in livestock
Feed additive composition. delete delete delete The method of claim 1,
Including the powdered pepper fruit,
The pepper seed powder is contained in an amount of 10 to 800 parts by weight based on 100 parts by weight of the pepper fruit stem
Feed additive composition. The method of claim 1,
Including the powdered pepper fruit,
Pepper seed powder is 10 to 800 parts by weight based on 100 parts by weight of the pepper fruit stem powder, and
Thistle extract is contained in 10 to 800 parts by weight
Feed additive composition. Feed comprising the feed additive composition according to claim 1.