KR102200016B1 - Composition comprising Green tea extract for caring damages of skin cells by microdust - Google Patents

Abstract

In the present specification, as a composition comprising green tea extract as an active ingredient, the expression level of one or more selected from the group consisting of KRT 1, CLDN 1, CLDN 4, and OCLN, which are genes in skin cells whose expression level is affected by fine dust, is Disclosed is a composition for skin damage care by fine dust that is controlled to a normal level.
By using the composition according to the present invention, it is possible to take care of damage to skin cells by returning the gene expression level changed by fine dust to a normal level.

Description

Composition comprising Green tea extract for caring damages of skin cells by microdust}

The present specification discloses a composition for treating skin cell damage caused by fine dust. In more detail, a composition comprising a tea tree extract is disclosed, which significantly changes a biomarker, which is a skin cell gene whose expression level is changed by fine dust compared to normal skin cells, to care for skin cell damage.

Recently, interest in fine dust has increased due to environmental issues, and the pollutant market is expanding. Fine dust is more harmful to the human body because it has smaller sized particles than general yellow sand, so it penetrates the human body better, and contains more harmful substances such as heavy metals, nitrates, ammonium and sulfates. When fine dust enters our body, cells responsible for the body's immunity act to remove the dust. As a side effect at this time, inflammation is accompanied, and in addition, the World Health Organization classifies fine dust as a group 1 carcinogens that have a direct relationship with cancer.

Fine dust penetrates not only the respiratory tract, but also into the skin or hair follicles, which is expected to cause skin troubles or hair loss, but the mechanism and target research on how it negatively affects the skin and hair is only incomplete, and the problem is solved accordingly. There is also no research on anti-pollution materials to do so.

Non-Patent Literature 1 discloses that the expression level of claudin 1 (CLDN 1), which is a major component of a tight junction, which is a granular layer barrier, decreased due to the stimulation of fine dust of PM 2.5.

Kim, H.J., et al, "Transcriptome analysis of airborne PM2.5-induced detrimental effects on human keratinocytes", Toxicology Letters 273, 26-35, 2017.

Accordingly, the present inventors have found that fine dust has a harmful effect on the skin, and by this effect, it affects the expression of skin cell genes, resulting in symptoms such as skin cell damage, and that green tea extract has the effect of restoring gene expression. Confirmed.

Accordingly, in one aspect, an object of the present invention is to provide a composition for treating damage to skin cells by fine dust comprising a green tea extract.

In order to achieve the above object, in one aspect, the present invention is a composition comprising a green tea extract as an active ingredient, keratin 1 (KRT 1), claudin 1 ( It provides a composition for skin damage care by fine dust that controls the expression level of one or more selected from the group consisting of CLDN 1), claudin 4 (CLDN 4), and occludin (OCLN) to a normal level.

In one aspect, by using the composition for skin damage care by fine dust provided by the present invention, it is possible to care for damage to skin cells by returning the gene expression level changed by fine dust to a normal level.

1 shows that the mRNA expression level of the KRT 1 gene was increased in skin cells stimulated by ERM-CZ120 fine dust, and returned to the normal level by green tea extract treatment.
2 shows an increase in the mRNA expression level of the CLDN 1 gene in skin cells stimulated by ERM-CZ120 fine dust, and returns to a normal level by treatment with green tea extract.
3 shows an increase in the mRNA expression level of the CLDN 4 gene in skin cells stimulated by ERM-CZ120 fine dust, and returns to the normal level by treatment with green tea extract.
4 shows an increase in the mRNA expression level of the OCLN gene in skin cells stimulated by ERM-CZ120 fine dust, and returns to a normal level by treatment with green tea extract.

Hereinafter, the present invention will be described in detail.

In one aspect of the present invention, the composition for care of skin damage caused by fine dust may include green tea extract as an active ingredient.

Tea tree ( Camellia sinensis ) is a perennial evergreen plant belonging to theaceae family. It is distributed in tropical, subtropical and temperate regions. The main components of the tea tree include catechins, caffeine, amino acids, vitamins and minerals, and these chemical components are various It is reported to exhibit physiological activity and pharmacological effect. Today, active research is being conducted as a physiologically active substance that contributes to health, and among them, catechins are reported to have various functions such as caries prevention effect, antioxidant effect, antibacterial effect and anticancer effect.

In one aspect, the green tea extract is a green tea leaf extract, the green tea leaves are not fermented, and internal enzymes may be inactivated.

In one aspect of the present invention, the green tea may be extracted with a specific extraction solvent to form a green tea extract.

In one aspect of the present invention, the green tea extract may be prepared by extracting green tea with water or an organic solvent. Specifically, green tea is selected from the group consisting of water, C ₁ -C ₆ anhydrous or aqueous lower alcohol, acetone, butylene glycol, ethyl acetate, diethyl acetate, diethyl ether, benzene, chloroform, and hexane. It can be prepared by extraction with an extraction solvent.

In one aspect, the green tea extract may be extracted at room temperature.

In one aspect, the green tea extract may be obtained by additionally at least one of evaporation, filtration, concentration, separation, or drying after being extracted with the extraction solvent. In particular, the green tea extract may have been subjected to one or more filtration processes.

In one aspect, the separation process may include a centrifugation process.

Specifically, the extraction includes water, an anhydrous or hydrous lower alcohol of C ₁ -C ₆ , a polar solvent including acetone and butylene glycol, and ethyl acetate, diethyl acetate, diethyl ether, benzene, chloroform, and hexane. Any one or more of the low polarity solvents may be used as the solvent. More specifically, the solvent may be a 10-90% aqueous ethanol solution, 10-50%, 15-45%, or 20-40% ethanol aqueous solution. When the solvent is a 20-40% ethanol aqueous solution, the active ingredient can be effectively extracted from green tea. In one embodiment, the solvent is about 30% aqueous ethanol solution.

In one aspect, the extract may be concentrated under reduced pressure to an appropriate temperature in a distillation apparatus equipped with a cooling condenser after extraction.

However, the green tea extract according to the present invention can be obtained by extraction by a conventional method in the art, and is not limited by the above-described method.

In one aspect of the present invention, in the composition, the composition may include green tea extract in an amount of 0.000001 to 30% by weight based on the total weight of the composition. When the content is 0.000001 to 30% by weight, the skin damage care effect due to fine dust by the green tea extract was excellent.

Specifically, 0.0000001 wt% or more, 0.0000005 wt% or more, 0.0000007 wt% or more, 0.0000009 wt% or more, 0.000001 wt% or more, 0.000002 wt% or more, 0.000004 wt% or more, 0.000006 wt% or more, 0.000008 wt% or more, 0.00001 weight % Or more, 0.00003% or more, 0.00005% or more, 0.00007% or more, 0.00009% or more, 0.0001% or more, 0.0003% or more, 0.0005% or more, 0.0007% or more, 0.0009% or more, 0.001% or more % Or more, 0.01% or more, 0.1% or more, 1% or more, 3% or more, 5% or more, 7% or more, 9% or more, 10% or more, 13% or more, 15% % Or more, 17 wt% or more, 19 wt% or more, 21 wt% or more, 23 wt% or more, 25 wt% or more, 27 wt% or more, 29 wt% or more, 30 wt% or more, 31 wt% or more, 32% or less, 31% or less, 30% or less, 29% or less, 28% or less, 26% or less, 24% or less, 22% or less, 20% or less, 18% or less, 16% or less, 14% or less, 12% or less, 10% or less, 9% or less, 8% or less, 6% or less, 4% or less, 2% or less, 1% or less, 0.1 wt% or less, 0.09 wt% or less, 0.04 wt% or less, 0.01 wt% or less, 0.006 wt% or less, 0.001 wt% or less, 0.0009 wt% or less, 0.0007 wt% or less, 0.00005 wt% or less, 0.00003 wt% or less, 0.00001 wt% or less, 0.000009 wt% or less, 0.000007 wt% or less, 0.000005 wt% or less, 0.000003 wt% or less, 0.000001 wt% or less Below, 0.0000009 wt% or less, 0.0000007 wt% or less, 0.0000005 wt% or less, 0.0000003 wt% or less, 0.0000002 wt% or less, 0.0000001 wt% or less, 0.00000009 wt% or less, but is not limited thereto.

In one aspect, the active ingredient of the composition according to the present invention may be one that acts on the tight junction of the skin barrier.

The close junction is a type of cell junction and is composed of occluding, claudin, tricellulin, junctional adhesion molecule (JAM), zona occluden (ZO), and the like. The close junction can be divided into a part contained in the cell membrane and an intracellular part. Occludin and claudin are close-junction proteins contained in cell membranes. Occludin is the first transmembrane protein found in tight junctions and has four transmembrane regions. Occludin is known not only as a constituent of tight junction, but also regulates the function of tight junction. Along with Occludin, claudin, a protein constituting a tight junction, also has four transmembrane regions. Close junction is a link between cells that fills the gaps between neighboring cells and cells, regulates the movement of small substances, regulates material permeation between cells and cells, and maintains the polarity of cells. Recently, it has been reported that close contact is playing an important role in skin barrier function through research results. Tight junction is a type of intercellular binding mode. It is a junction between cells that appears mainly in epithelial cells and endothelial cells, and forms a complex network having a string-like structure. Tight junction has two main functions from this structure. The first is the barrier function, which prevents the leakage of substances between cells constituting epithelial cells or endothelial cells. It is mainly dealt with importantly in the blood vessels or digestive tract. The second is the fencing function, which is the function of enclosing a string-shaped fencing from cell membrane lipids to partition the cell membrane. Therefore, the active ingredient of the composition according to the present invention acts on the tight junction of the skin barrier to enhance the barrier function and barrier function of skin cells, thereby treating the damaged skin by stimulation of fine dust.

Another aspect of the present invention includes the use of the composition to care for skin damage caused by fine dust.

As used herein, the term "fine dust" refers to a particulate matter that is invisible to our eyes and floats or scatters for a long time in the atmosphere. Specifically, in the present invention, the particle size of the fine dust may be 10 μm or less in particle diameter (PM 10), specifically 2.5 μm or less in particle diameter (PM 2.5). Particularly, particulate matter having a particle diameter of 2.5 μm (PM 2.5) or less is referred to as "ultra-fine dust", and in this specification, "fine dust" is intended to include "ultra-fine dust."

In one aspect, the fine dust in the present invention may include one or more of arsenic, cadmium, lead, and nickel. The heavy metal components such as arsenic, cadmium, lead, and nickel are included together in fine dust and attack skin cells, causing skin allergy, pigmentation, and inflammation to prevent skin aging. Can trigger. In one embodiment, the fine dust includes arsenic, cadmium, lead, and nickel.

The term "care" as used herein refers to effectively protecting skin cells from stimulation, and inhibiting, preventing, and restoring (restore) a change in the expression level of a specific gene by the stimulation. Here, "recovery" also includes improving the expression level above the reference in addition to changing the expression level close to the reference when the expression level of the gene in the absence of stimulation is based on the reference.

In one aspect, the present invention provides a composition for suppressing damage to skin cells by fine dust by controlling the expression level of a specific gene in skin cells damaged by fine dust to a normal level.

Specifically, in the present invention, genes in skin cells whose expression levels are affected by fine dust include keratin 1 (KRT 1), claudin 1 (CLDN 1), claudin 4 (CLDN 4), and occludin (OCLN). It may include one or more selected from the group. The keratin 1 (KRT 1), claudin 1 (CLDN 1), claudin 4 (CLDN 4), and occludin (OCLN) are genes whose expression levels are reduced by fine dust, so they suppress the expression levels of these genes to a normal level. By controlling it, damage to skin cells can be suppressed.

Genes used in the present invention whose expression levels are changed by fine dust are shown in Table 1 below. [Table 1] shows genes whose expression level is reduced by fine dust. In these tables, Gene Symbol means the official gene symbol, and Gene title means the name of each gene. These contents can be confirmed through the description in Non-Patent Document 1.

Reduction gene Gene
Symbol Gene title KRT 1 Keratin 1 CLDN 1 Claudin 1 CLDN 4 Claudin 4 OCLN Occludin

Analysis of the expression level of the gene or protein includes microarray, PCR, NGS (Nest Generation Sequencing; next-generation sequencing), Western blot, Northern blot, ELISA, radioimmunoassay, radioimmuno diffusion method, tissue immunostaining, immunoprecipitation analysis, etc. It can be analyzed using a variety of analysis methods known in the art.

Skin cells are damaged by fine dust, which induces inflammation and further damages the skin cells. Green tea extract can improve the skin condition by taking care of the vicious cycle of skin cell damage.

In one aspect of the present invention, the composition may be a cosmetic composition, a pharmaceutical composition, or a health functional food composition.

The cosmetic composition includes, for example, various creams, lotions, various creams, lotions, and cosmetics such as skins, cleansing, face wash, soap, and beauty liquids.

In one aspect, the cosmetic composition to which the composition containing the green tea extract of the present invention is added may take the shape of a solution, an emulsion, a viscous mixture, or the like.

That is, in one aspect, the cosmetic composition of the present invention is not particularly limited in its formulation, for example, emulsion, cream, lotion, essence, pack, gel, powder, makeup base, foundation, lotion, ointment, patch, essence, cleansing Formulations such as foam, cleansing cream, cleansing water, body lotion, body cream, body oil, body essence, shampoo, conditioner, body cleaner, soap, hair dye, spray, etc. may be mentioned.

In the cosmetic composition of each formulation, other ingredients other than the green tea extract may be appropriately selected and blended by a person skilled in the art without difficulty according to the formulation or purpose of use of other cosmetic materials.

In addition, in one aspect, the cosmetic composition of the present invention may include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingo lipids, and seaweed extract.

In one aspect, in the cosmetic composition of the present invention, in addition to the essential ingredients, other ingredients which are usually blended in the cosmetic may be blended as needed.

Other ingredients that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, Blood circulation accelerators, cold sensation agents, restrictors, and purified water.

In addition, the blending components that may be added are not limited thereto, and any of the above components can be blended within a range that does not impair the object and effect of the present invention.

In one aspect, the pharmaceutical composition comprising the green tea extract of the present invention may further include a suitable carrier, excipient, and diluent commonly used in the preparation of pharmaceutical compositions.

As the dosage form, the pharmaceutical composition containing the green tea extract may be formulated according to a conventional method, including oral preparations such as tablets, capsules, powders or syrups, or external preparations such as ointments, gels, creams, patches or sprays, etc. It can be formulated and used in any form suitable for pharmaceutical preparations.

In general, it should be understood that the actual dosage of the active ingredient administered by the pharmaceutical composition should be determined in light of various related factors such as the severity of symptoms, the selected route of administration, the age, sex, weight and health status of the subject. In general, the dosage of the active ingredient may be 0.0001 mg/kg/day to 3000 mg/kg/day, for example, 10 mg/kg/day to 500 mg/kg/day.

In the health functional food composition as an aspect of the present invention, the health food is manufactured using nutrients that are easily deficient in daily meals or raw materials or ingredients (functional ingredients) having useful functions for the human body, and maintains the normal function of the human body Or it may refer to food that maintains and improves health through activation of physiological functions, but is not limited thereto. The health food may be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills, etc., but is not limited thereto and may be manufactured and processed in any form according to laws.

Specifically, the health drink composition is not particularly limited to other ingredients other than containing the compound as an essential ingredient in the indicated ratio, and may contain various flavoring agents or natural carbohydrates as an additional ingredient, such as a conventional beverage. Examples of natural carbohydrates are conventional sugars such as monosaccharide polysaccharide, cyclodextrin, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used.

In general, it should be understood that the actual dosage of the active ingredient administered by the health functional food composition should be determined in light of various related factors such as the severity of symptoms, the selected route of administration, the age, sex, weight, and health status of the subject. . In general, the dosage of the active ingredient may be 0.0001 mg/kg/day to 1000 mg/kg/day, for example, 0.02 mg/kg/day to 6 mg/kg/day.

Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to examples. However, these examples are provided for illustrative purposes only to aid understanding of the present invention, and the scope and scope of the present invention are not limited by the following examples.

[Example 1] Preparation of green tea extract

Green tea extract was prepared using green tea obtained from Amorepacific Group, Jangwon. The collected green tea leaves were washed with purified water, filtered and dried, mixed with 30% ethanol (EtOH) in a ratio of 7 weight of 1:8, stirred at 25° C. for 72 hours, and extracted in a size of 250 mesh.

The obtained extract was filtered three times, each with a size of 3.0 μm or less, 1.0 μm or less, and 0.3 μm or less. The filtered extract was precipitated at 4° C. for 72 hours, and then filtered again to a size of 0.2 μm or less to prepare a green tea extract.

[Experimental Example 1] Gene expression level measurement experiment through real-time qPCR quantification

1. Cultivation of keratinocyte line

Keratinocyte cell line (Human normal epidermal keratinocytes) was Lonza社(Lonza, Inc. Walkersville, Maryland material) was purchased and subcultured in a culture under a CO ₂ incubator (CO ₂ incubator), 37 ℃ from, 5% CO ₂ conditions I did. In 500ml of KBM-2 (KBMTM-2, CC-3103) medium, BPE (Bovine pituitary extract), human epidermal growth factor (hEGF), insulin, hydrocortisone, gentamicin sulfate (Gentamycin Suflate), epinephrine (Epinephrine), a culture medium containing transferrin (Transferrin) was used.

Confluency of up to 90% was maintained during cultivation, and subcultivation was performed thereafter.

2. Treated keratinocytes with fine dust and green tea extract

For fine dust, ERM-CZ120 fine dust commercially available from Sigma-Aldrich was used, and fine dust of PM 10 level and arsenic, cadmium, lead, and nickel ).

A 6-well plate was used and '1. Cell lines cultured under the condition of cultivation of keratinocytes' cell lines were cultured under the conditions of 3 × 10 ⁵ cells/well, respectively, as a control group, untreated group (control group 1), and a group treated with 50 ppm of fine dust (Control group 2), and a group treated with 50 ppm of fine dust and green tea extract, respectively (Example 1), a total of three groups were subjected to material treatment and incubated for 3 hours and 6 hours.

Experimental group fine dust
( ERM -CZ120 Fine Dust) Green tea extract Control 1
(Fine dust not treated) - - Control 2
(Fine dust treatment) 50 ppm - Example 1
(Fine dust + green tea extract treatment) 50 ppm 50 ppm

3. Real-time qPCR quantification

3-1. RNA extraction process

'2. Remove the culture solution from Control 1, Control 2, and Example 1 obtained from'treatment of fine dust and green tea extract on keratinocyte line', and wash the cells with 2 ml of Phosphate Buffered Saline (PBS), and then trizol reagent ( Trizol reagent, Invitrogen, Carlsbad, CA, USA) was used to isolate intracellular RNA.

3-2. cDNA synthesis process

The isolated RNA was purified once more with QIAGEN's RNA kit (QIAGEN RNeasy kt, QIAGEN, Valencia, CA), and then Agilent's Bioanalyzer 2100 model instrument (Agilent 2100 BioAnalyzer, Agilent Technologies, Santa Clara, CA, USA ) Was used to check the quality of RNA. CDNA was synthesized from RNA using Invitrogen's Superscript Reverse Transcriptase (RT) kit, Invitrogen, Carlsbad, CA.

3-3. qPCR performance

This was quantitatively analyzed through a real time-reverse transcription polymerase chain reaction (Q-RT-PCR) using the primers of [Table 3]. Gene expression pattern changes were evaluated by real-time PCR using Applied Biosystems' TaqMan gene expression system (TaqMan gene expression assay kit, Applied Biosystems, Foster City, CA), and the results were evaluated by real-time PCR. To [Fig. 4]. Both Q-RT-PCR and real-time PCR used were carried out according to the standard protocol distributed by Life Technology, and specifically, after treatment at 95°C for 20 seconds, the process of treating 3 seconds at 95°C and 30 seconds at 60°C Was performed for 40 cycles.

As shown in [Fig. 1] to [Fig. 4], the control group 2 treated with the fine dust at a concentration of 50 ppm was compared to the control group 1 without treatment with the fine dust keratin 1 (Keratin 1), claudin 1 (Claudin 1), claudin 4 (Claudin 4) and occludin (Occludin) mRNA expression levels were confirmed to decrease. Specifically, the mRNA expression level of keratin 1 was 0.19 when treated for 3 hours, and 0.13 when treated for 6 hours, and it was confirmed that the mRNA expression level was significantly reduced. Claudin 1 was 0.44 and 0.24, respectively, and claudin 4 was both 0.52. , It was confirmed that occludin decreased to 0.48 and 0.39, respectively. That is, the expression levels of the keratinocyte differentiation marker (ie, keratin 1) and the tight junction components (ie, claudin 1, claudin 4, and occludin), which are granular barriers, were all decreased by fine dust.

Example 1 shows that the green tea extract of the present invention can care for the mRNA expression level of the markers reduced by fine dust. Specifically, FIG. 1 shows that the mRNA expression level of keratin 1 is 0.79 when treated for 3 hours, and 0.99 when treated for 6 hours, indicating that the decreased mRNA expression level by fine dust is restored, and FIG. 4 is the mRNA expression level of occludin Is 0.88 when treated for 3 hours and 0.98 when treated for 6 hours, indicating that the decreased mRNA expression level by fine dust is recovered. In particular, claudin 1 and claudin 4 were 1.12 and 1.23, respectively, when treated for 3 hours, and 1.23 and 1.45, respectively, when treated for 6 hours, respectively, showing improved mRNA expression levels compared to control 1 (see FIGS. 2 and 3).

Therefore, it can be seen that the expression levels of keratin 1, claudin 1, claudin 4, and occludin decreased in skin cells stimulated by fine dust can be recovered by treatment with green tea extract.

Hereinafter, examples of the formulation of the composition according to the present invention will be described, but cosmetic compositions, pharmaceutical compositions, and health functional food compositions can be applied in various formulations, which are not intended to limit the present invention, but are intended to be described in detail. .

[Formulation Example 1] Tablet

After mixing 100 mg of green tea extract, 400 mg of lactose, 400 mg of corn starch and 2 mg of magnesium stearate according to an embodiment of the present invention, tablets were prepared by tableting according to a conventional tablet preparation method.

Ingredients Content(mg) Green tea extract 100 Lactose 400 Corn starch 400 Magnesium stearate 2

[Formulation Example 2] Capsule

After mixing 100 mg of green tea extract, 400 mg of lactose, 400 mg of corn starch, and 2 mg of magnesium stearate according to an exemplary embodiment of the present invention, a capsule was prepared by filling in a gelatin capsule according to a conventional capsule preparation method.

Ingredients Content(mg) Green tea extract 100 Lactose 400 Corn starch 400 Magnesium stearate 2

[Formulation Example 3] Granules

50 mg of green tea extract, 250 mg of anhydrous crystalline glucose, and 550 mg of starch according to an embodiment of the present invention were mixed, molded into granules using a fluid bed granulator, and then filled into a cloth.

Ingredients Content(mg) Green tea extract 50 Anhydrous glucose 250 Starch 550

[Formulation Example 4] Soap

Ingredients content(%) Green tea extract 5.00 maintain Appropriate amount Sodium hydroxide Appropriate amount Sodium chloride Appropriate amount Spices Appropriate amount Purified water Balance

[Formulation Example 5] Lotion

Ingredients content(%) Green tea extract 5.00 L-ascorbic acid-2-phosphate magnesium salt 1.00 Water-soluble collagen (1% aqueous solution) 1.00 Sodium citrate 0.10 Citric acid 0.05 Licorice extract 0.20 1,3-butylene glycol 3.00 Purified water Balance

[Formulation Example 6] Cream

Ingredients content(%) Green tea extract 3.00 Polyethylene glycol monostearate 2.00 Self-emulsifying type glycerin monostearate 5.00 Cetyl alcohol 4.00 Squalene 6.00 Glyceryl tri2-ethylhexanoate 6.00 Sphingoglycolipid 1.00 1.3-butylene glycol 7.00 Purified water Balance

[Formulation Example 7] Ointment

Ingredients content(%) Green tea extract 5.00 Polyvinyl alcohol 13.00 L-ascorbic acid-2-phosphate magnesium salt 1.00 Lauroylhydroxyproline 1.00 Water-soluble collagen (1% aqueous solution) 2.00 1,3-butylene glycol 3.00 ethanol 5.00 Purified water Balance

[Formulation Example 8] Preparation of essence

Ingredients content(%) Green tea extract 3.00 Hydroxyethylene cellulose (2% aqueous solution) 12.00 Xanthan gum (2% aqueous solution) 2.00 1,3-butylene glycol 6.00 Dark glycerin 4.00 Sodium hyaluronate (1% aqueous solution) 2.00 Purified water Balance

[Formulation Example 9] Health Food

Ingredients content Green tea extract 2mg Vitamin A acetate 70μg Vitamin E 1.0mg Vitamin B1 0.13mg Vitamin B2 0.15mg Vitamin B6 0.5mg Vitamin B12 0.2μg Vitamin C 10mg Biotin 10μg Nicotinic acid amide 1.7mg Folic acid 50μg Calcium pantothenate 0.5mg Ferrous sulfate 1.75mg Zinc oxide 0.82mg Magnesium carbonate 25.3mg Potassium Phosphate Monobasic 15mg Dicalcium phosphate 55mg Potassium citrate 90mg Calcium carbonate 100mg Magnesium chloride 24.8mg

[Formulation Example 10] Health Drink

Ingredients content Green tea extract 50mg Citric acid 1000mg oligosaccharide 100 g Taurine 1g Purified water Balance

Claims (11)

Containing green tea extract as an active ingredient, fine Cosmetic composition for the care of skin damage caused by dust. Containing green tea extract as an active ingredient, fine Health functional food composition for care of skin damage caused by dust. The method according to claim 1 or 2,
The green tea extract is contained in an amount of 0.000001 to 30% by weight based on the total weight of the composition. The method according to claim 1 or 2,
The green tea extract is water, any one or more extraction solvents selected from the group consisting of water, C ₁ -C ₆ anhydrous or aqueous lower alcohol, acetone, butylene glycol, ethyl acetate, diethyl acetate, diethyl ether, benzene, chloroform and hexane The composition is extracted with. The method according to claim 1 or 2,
The active ingredient of the composition is to act on the tight junction of the skin barrier, the composition. The method according to claim 1 or 2,
The composition, wherein the fine dust comprises one or more of arsenic, cadmium, lead, and nickel. The method according to claim 1 or 2,
The fine dust has a particle size of 10 PM or less, composition. The method of claim 2,
The green tea extract is consumed in an amount of 0.02 to 6 mg / kg / day, composition. delete delete delete

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