KR102197866B1 - Composition of cosmetic application containing illite eluate for inhibiting secretion of sebum, treating acne or soothing skin - Google Patents

Abstract

The present invention relates to a cosmetic composition containing an illite extract alone or a natural material having a synergistic effect and having an excellent sebum secretion inhibitory effect, acne prevention and amelioration effect, and skin soothing effect. The present invention provides a cosmetic composition containing illite eluate obtained by condensing purified water vapor generated by heating illite and purified water in a heating furnace, or making purified water vapor pass through illite and then condensing the vapor. The cosmetic composition exhibits an effect of inhibiting sebum secretion, ameliorating acne, or soothing skin.

Description

Composition of cosmetic application containing illite eluate for inhibiting secretion of sebum, treating acne or soothing skin}

The present invention relates to a cosmetic composition containing illite eluate as the main active ingredient, and more specifically, illite eluate alone or containing natural ingredients having a synergistic effect, and has sebum secretion inhibitory effect, acne prevention and improvement effect, skin soothing It relates to a cosmetic composition having excellent effect.

In general, the production of sebum in the skin, including the scalp and face, plays a role in maintaining moisturization of the skin or preventing the invasion of microorganisms, but excessive secretion of sebum causes the skin to become shiny, lift the makeup, enlarge pores, and post-puberty. It may also trigger the promotion of acne formation. There are several causes involved in the excessive secretion of sebum. Among them, the cause of the activation of sebaceous gland cells by the action of hormones is known as the most important factor. Conventionally, female hormones such as estrogen have been used to improve the secretion of excess sebum or acne caused by male hormones. However, due to the occurrence of skin inflammation and side effects from hormone administration, the use is discontinued or very trace amounts are currently used. In addition, even in the cosmetic field, the research is inadequate, although excessive secretion of sebum is a factor of great interest as a factor of lifting of makeup or enlargement of pores. Currently, the method of suppressing the secretion of sebum in cosmetics is the use of porous powder that temporarily adsorbs sebum, or extracts that are known to suppress sebum, but the content of the active ingredient is insignificant, so the effect is insignificant. .

On the other hand, peroxisome proliferative activating receptors (PPARs) are a known class of nuclear hormone receptors having three subtypes α, β/δ, and γ of various tissue distribution. The peroxisome proliferative activating receptor subtype α (hereinafter referred to as'PPAR-α') was initially identified based on the regulation of the gene encoding the fatty acid oxidase by reacting with a peroxisome proliferative factor such as a derivative of fibric acid. (Issemann and Green, Nature, 1990, 347: 645-650). In addition, the literature (Leone et al., Proc. Natl. Acad. Sci., USA, 1999, 96: 7473-7478) demonstrates the important role of fatty acids in tissues performed by PPAR-α. Lipid activators of PPAR-α such as linoleic acid and the like are known in the art. They have been demonstrated to promote skin epithelial barrier formation in vitro (Hanley et. al., J. Clin. Inv., 1977, 100: 705-712). In addition, activators of PPAR-γ have been found to increase insulin sensitivity in recent animal experiments, as well as reduce the amount of glucose and insulin, suggesting the potential as an ameliorating agent for diabetes and some complications thereof (Ricote M. , Nature, 1998, 391: 79-82). In addition, PPAR-γ has been reported to affect the maturation of sebocytes in the sebaceous gland (Kuenzli et. al., British J. Derm., 2003, 149: 229-236). , There are few examples of using this in the art. In addition, International Publication No. WO2003/068205 refers to foods that can prevent metabolic diseases such as hypertension and cholesterol by PPAR-α and PPAR-γ agonists, but reference to PPAR-γ inactivating agents is No, it also limits the use of the composition to metabolic diseases.

In addition, Korean Patent Application No. 10-2005-0049162 discloses that an extract obtained by extracting pine needles, Myungil leaves (Shinseoncho), kale, green tea, and spirulina as a common organic solvent is effective in improving acne, and Korean Patent Application No. 10 -2006-0039235 discloses that the composition of 90% by weight of the extract obtained by hot water or ethanol extraction of upper leaves, upper leaves, mulberry and mulberry has the effect of promoting skin soothing. have.

In order to solve such skin problems, cosmetics using natural products have been developed in recent years to reduce skin irritation caused by various chemical substances. Natural ingredients not only have fewer side effects on the skin, but recently, as consumers' response to cosmetics using natural ingredients increases, the value of development as a cosmetic ingredient is further increasing.

1. International Publication No. WO2003/068205 2. Korean Patent Application No. 10-2005-0049162 3. Korean Patent Application No. 10-2006-0039235

The present invention provides a method for manufacturing cosmetics having a predetermined functionality by confirming the sebum secretion inhibitory effect, acne prevention and improvement effect, and skin soothing effect of illite eluate, developing a method of use thereof, and using this raw material It aims to do.

In addition, an object of the present invention is to provide an illite eluate showing an effect of inhibiting sebum production by reducing the activity of PPAR-γ, which affects the maturation of sebaceous cells in sebaceous glands when applied to skin cosmetics.

In addition, it is an object of the present invention to provide a cosmetic comprising an illite eluate exhibiting a 5-alpha-reductase inhibitory effect having an excellent anti-acne effect and an anti-hair loss effect.

In order to solve the above technical problem, the present invention

Condensing the purified water vapor generated by placing ilite and purified water in a heating furnace or condensing the vapor after passing purified water vapor through ilite to condense sebum secretion, acne improvement effect, or skin soothing effect It provides a cosmetic composition characterized in that it represents.

In the present invention as described above, when preparing the illite eluate, the illite may further include dried sprouts and/or basalt powder.

As described above, the illite eluate according to the present invention has the effect of reducing sebum cell differentiation and sebum accumulation, reducing skin shine due to excessive sebum secretion, and thus preventing acne and soothing skin.

Accordingly, it can be seen that cosmetic compositions such as lotions, creams, emulsions, packs, and powders containing the illite eluate have excellent cosmetic effects such as improving sebum secretion, preventing and improving acne, hair growth, and soothing skin.

Hereinafter, specific details for carrying out the present invention will be described.

The present invention relates to a composition used as an active ingredient in a cosmetic composition because illite eluate has excellent sebum secretion inhibitory effect, acne prevention and improvement effect, and skin soothing effect.

In addition, the present invention may further include a dried cheongsam in ilite in order to increase the effect.

In addition, the present invention further enhances the sebum secretion inhibitory effect, acne prevention and improvement effect, and skin soothing effect by further including basalt among minerals such as illite.

Illite used in the present invention is one of clay minerals, and is a fine mica-group mineral that occurs in tuff-like volumetric rocks that are not rich in aluminum. In terms of chemical composition, some are close to muscovite mica, but relatively SiO ₂ , MgO, H ₂ O are high and K ₂ O is low. It is a mineral of the same family as hundreds of mica. Hardness 1-2, specific gravity 2.6-2.9. The cleavage is complete with [001], the streak color is white, and there is a earthy gloss. There is a view that it is not an independent mineral in terms of its chemical composition or crystal structure, but a mixed mineral containing other components. It is produced in aluminum-rich heterogeneous or tuff-like sedimentary rocks, and is produced as a metamorphic mineral of a hydrothermal mineral bedrock.

In the present invention, the sprouts used in the present invention are dicotyledonous plants, as a biennial plant of the Lamiaceae family, whose scientific name is Lamium amplexicaule. It grows in grass or on wet roadsides. It is about 30cm high. The stem is chamfered, pruned, and lying at an angle. Leaves are 5~10cm long and 3~8cm wide, facing each other, and the lower leaves are long and round petioles. The upper leaf has no petiole and completely surrounds the stem on both sides. There are hairs on the front and back veins of the leaves, and there are serrations on the edges, and red purple flowers bloom in April-May as if they have turned several on the axils. The calyx is about 5mm at the end, is divided into 5 branches, and has fine hairs. The corolla has a long corolla, the lower lip petal is split into three, and the upper lip petal is slightly bent forward. Two of the four stamens are long, and closed flowers are common.

In the present invention, the outpost including the root of the sprout sprout is used. It is safe to use with flowers during flowering.

Also. Basalt is a volcanic rock mainly composed of basic plagioclase, pyroxene, and olivine. It is a black eruption rock containing less than 52% by weight of SiO ₂ . Because of this low silica content, the basalt lava flow is low in viscosity and flows quickly and flows more than 20 km from the crater. Basalt is generally classified into Tholeiiti basalt, Highalumina basalt, and alkali basalt. Among the erupting rocks, the most common basalt constitutes most of the oceanic crust, and in Korea, it is distributed in Jeju Island, southern Mt. Baekdu, Cheorwon in Gangwon-do, Ulleungdo in Gyeongsangbuk-do, and Hantan River in Gyeonggi-do. In the world, an average of 800m thick lava plateau is formed over 500,000 km2 in the Decan Highlands of India. In addition, the basalt of the Columbia River in the United States reaches a thickness of 1,800 m. When basalt is fresh, it is mostly gray-black or black, fine-grained and looks dense, but sometimes it is porous.

The illite eluate may be prepared through the following process.

The method for preparing an illite eluate according to the present invention comprises a grinding step, an elution step, and a condensation step.

In the pulverization step, the illite mineral is pulverized so that the extraction of the active ingredient of ilite can be better in the elution step. For this, the size of the crushed illite is suitable 0.1-0.5mm. If it is less than 0.1mm, there is a problem that the burden of grinding cost increases, and if it exceeds 0.5mm, there is a problem that the surface area in contact with the solvent is relatively small, and thus elution is not properly performed. The crushing of illite minerals may be performed using a conventional method of crushing minerals.

The elution step is a step of eluting the active ingredient of the illite mineral. The elution method can be largely divided into two.

In the first method, the illite and purified water pulverized in the pulverization step are placed in a heating furnace and heated to elute the active ingredient from the pulverized illite mineral.

It is preferable to maintain the heating furnace temperature at 185 to 400°C during elution. 185°C is a temperature at which purified water begins to boil, and below the temperature, extraction of extracted minerals is hardly performed, and when it exceeds 400°C, there is a problem that illite is denatured. In addition, the duration of the extraction step is not particularly limited, but it is preferable to proceed for 30 minutes to 2 hours.

The second method is to separately boil purified water to generate steam, and to contact the generated purified water vapor with pulverized illite to elute the active ingredient of illite.

Even at this time, the elution time is not particularly limited, but it is preferable to proceed for about 30 minutes to 2 hours.

Lastly, in the condensation step, the steam generated in the elution step is condensed, and the high-temperature steam is liquefied through the condensation step to become the illite elution purified water we want.

In addition, in order to increase the efficacy of the illite eluate according to the present invention, it may further include a dried sprout sprout pulverized to the same particle size as ilite.

In the case of further including dried sweet sprouts, it may have a better effect than when elite alone is eluted.

In addition, in order to increase the efficacy of the illite eluate according to the present invention, basalt pulverized into illite may be included. If these are further included, active ingredients such as eluted minerals are further increased, more than when only illite is eluted. It can have an excellent effect.

The composition of the present invention can be formulated into a composition such as a capsule, liquid, ointment, patch, or sustained-release agent using an acceptable carrier, and an acceptable base, vehicle, excipient, binder (e.g. starch, trager Cans rubber, gelatin, molasses, polyvinyl alcohol, polyvinyl ether, polyvinyl pyrrolidone, hydroxypropyl cellulose, methyl cellulose, ethyl cellulose and carboxymethyl cellulose), grinding agents (e.g. agar, starch, velatin powder, Sodium carboxymethyl cellulose, calcium carboxymethyl cellulose, crystalline cellulose, calcium carbonate, sodium hydrogen carbonate and sodium alginate), lubricants (e.g., magnesium stearate, talc, hydrogenated vegetable oil) and colorants. As the carrier and excipient, lactose, glucose, sucrose, mannitol, potato starch, corn starch, calcium carbonate, calcium phosphate, and cellulose are used. In addition to the above, additives such as a stabilizer, a dissolution aid, an adjuvant such as a transdermal absorption accelerator, and an additive such as a preservative may be further added.

The cosmetic composition according to the present invention has an excellent effect of inhibiting the production of sebum by reducing the activity of the peroxisome proliferator activating receptor subtype gamma (PPAR-γ), and exhibits an excellent effect of improving skin shine.

Since PPAR activation/inactivation can be demonstrated, a widely known method for confirming the activation effect of PPAR is a reporter gene assay (Kliewer et. al., Nature, 1992, 358: 771-774). The reporter gene analysis involves cloning a nucleotide sequence containing a promoter and reporter gene of interest, injection into eukaryotic cells, activating transcription, and It is an assay that controls expression. In this case, as the promoter is activated, the expression of the protein increases. The first reporter gene used was the CAT (chloramphenicol acetyltransferase) gene, and recently, the luciferase gene is most commonly used. In the present invention, the Dual-Luciferase Reporter Assay System Kit provided by Promega was used.

In addition to the composition related to the present invention, among these formulations, ingredients that can be blended into woolen products can be blended in the fields of pharmaceuticals, quasi-drugs, and cosmetics. For example, drugs such as vitamin E and derivatives thereof having a blood circulation promoting action, hormones such as estradiol and estrone having an anti-androgenic action may be mentioned.

Further, vasodilators such as alkoxycarbonylpyridine N-oxide and acetylcholine derivatives; Skin stimulating agents such as cephalantin; Antibacterial agents such as hexachlorophene, undecylenic acid, trichlorocarbanide, and bitionol; Zinc and its derivatives; Lactic acid or its alkyl ester; Organic acids such as citric acid; Protease inhibitors, such as tranekisamusan, etc. can be blended.

Further, alcohols such as ethanol and isopropyl alcohol; Polyhydric alcohols such as glycerin, propylene glycol, and polyethylene glycol; Oils such as higher fatty acids, higher alcohols, hydrocarbon oils, natural oils, ester oils, and silicone oils; Surfactants; Spices; Chelating agents; Moisturizing agents such as 1,3-butylene glycol, hyaluronic acid and derivatives thereof, maltitol, atherocollagen, and sodium lactate; Thickeners such as carboxyvinyl polymer; Antioxidants; Ultraviolet absorbers; Pigment; water; Ingredients, such as stabilizers, that are usually blended in the woolen agent can be blended within a range that does not impair the effects of the present invention.

The composition prepared as described above can be applied to the skin once to several times a day depending on the symptoms, and the application can be adjusted according to the degree of improvement of the symptoms.

Hereinafter, the present invention will be described in more detail with reference to test examples and examples, but the present invention is not limited to these examples.

[ Test example One]

<Production Example 1>

The illite mineral is pulverized to a diameter of 0.3mm, and 300g of the pulverized illite mineral and 500g of purified water are put in a heating furnace, and heated at 200℃ for 20 minutes to generate purified water vapor. Was condensed to obtain an illite eluate. After cooling to room temperature, an illite eluate was prepared.

<Production Example 2>

Preparation Example 2 was prepared in the same manner as in Preparation Example 1, except that 150 g of dried sprouts crushed to the same particle size as the illite mineral was further included, and the amount of illite was reduced to 150 g.

< Manufacturing example 3>

Preparation Example 3 was prepared in the same manner as in Preparation Example 1, except that 150 g of basalt pulverized to the same particle size as the illite mineral was further included and the amount of illite was reduced to 150 g.

< Manufacturing example 4>

Preparation Example 4 was prepared in the same manner as in Preparation Example 1, except that 50 g of dried sprouts crushed to the same particle size as the illite mineral and 100 g of basalt were further included, and the amount of illite was reduced to 150 g.

[ Test example 2] One-Person One-Time Experiment (OFAT) through PPAR Measurement of the activation ability of -γ

PPAR-γ activation ability measurement experiment was carried out as follows.

CV-1 cells (ATCC CCL 70), a monkey kidney epithelial cell line, were subcultured in DMEM medium containing 10% fetal calf serum treated with charcol/dextrin, and the effect of estrogen of phenol red was eliminated. To do this, a phenol red medium was used. Plasmid has a universal promoter expressed under normal culture conditions followed by a PPAR- (PPARs responsive element), which is activated by binding of PPAR- (gene and ligand-binding PPAR-) as a promoter, and as a reporter behind it. A plasmid with a firefly luciferase gene serving as a function, and a plasmid to which the Renilla luciferase gene used as a reference was conjugated was used.

CV-1 cells were spread on a 24-well plate at a concentration of 5×10 ⁴ cells and cultured for 24 hours, and then the three types of plasmid genes were transiently transformed using Promega's TransFast Kit. Next, after incubation for 24 hours, washing with 1X phosphate buffered saline (PBS), candidate substances were treated at appropriate concentrations, cultured again for 24 hours, and washed twice with 1X PBS. Next, after breaking the cells with 1X PLB (passive lysis buffer, Promega), the luciferase activity of the sample and reference was measured using the Dual-Luciferase Reproter Assay System Kit (Promega). In this experiment, the positive control group used pioglitazone, which is known to be the most potent among the ligands of PPAR-γ, and the negative control group was the DMSO used to dissolve the samples and the untreated group. The calculated value was taken as the ratio with the negative control group, and the results for the three repeated experiments are shown in Table 1 below.

PPAR-γ activity 10㎍/㎖ 100㎍/㎖ Manufacturing Example 1 0.85 0.72 Manufacturing Example 2 0.81 0.69 Manufacturing Example 3 0.79 0.65 Manufacturing Example 4 0.72 0.59 Negative control One One Positive control
0.1㎍/㎖ 1㎍/㎖ 10㎍/㎖ 1.2 3.8 5.4

As can be seen from Table 1, the composition of the present invention exhibited excellent PPAR-γ activity inhibitory effect.

[ Test example 3] Test on the effect of improving skin oiliness through inhibition of sebum secretion

In order to confirm the sebum production suppression and skin shine improvement effect of the present invention, 26 males and females aged 14-28 were divided into two groups, and the composition of Table 2 was used in the same manner as the daily lotion use for 4 weeks. The degree of improvement was determined according to the following evaluation criteria. The determination results are shown in Table 3 below.

<Evaluation criteria>

+++: The secretion of sebum is significantly reduced and has a very good improvement effect.

++: Significant improvement effect

+: There is a slight improvement effect

ㅁ: No improvement effect, but no worsening

-: No improvement effect, rather worse

Ingredients (content: wt%) In implementation 1 Example 2 Example 3 Example 4 Comparative Example 1 Manufacturing Example 1 2.0 - Manufacturing Example 2 2.0 - Manufacturing Example 3 2.0 - Manufacturing Example 4 2.0 - Multi Wax 3.6 3.6 3.6 3.6 3.6 Steric acid 0.5 0.5 0.5 0.5 0.5 Floating paraffin 5.0 5.0 5.0 5.0 5.0 Dhigh Deg Wax 0.8 0.8 0.8 0.8 0.8 Bee Wax 17 0.05 0.05 0.05 0.05 0.05 Nicole MGS-B 1.9 1.9 1.9 1.9 1.9 Aricel #165 0.7 0.7 0.7 0.7 0.7 glycerin 7.0 7.0 7.0 7.0 7.0 Methylparaben 0.12 0.12 0.12 0.12 0.12 Profile Paraben 0.03 0.03 0.03 0.03 0.03 Liquid Paraben 5.0 5.0 5.0 5.0 5.0 Twin60 0.5 0.5 0.5 0.5 0.5 Carbomer 0.12 0.12 0.12 0.12 0.12 Purified water To 100 To 100 To 100 To 100 To 100

term Example 1 Example 2 Example 3 Example 2 Comparative Example 1 1 week + ++ ++ ++ ㅁ 2 weeks ++ ++ ++ +++ ㅁ 3 weeks ++ +++ +++ +++ ㅁ 4 weeks +++ +++ +++ +++ +

As can be seen from Table 3, it was found that in the Example containing the composition according to the present invention, the sebum production inhibitory effect and the skin shine improvement effect were excellent, and the best effect was shown in Example 4.

[ Test example 4] Antibacterial activity test against acne bacteria

In this test example, a Paper Disc Test was performed to confirm the antibacterial activity of the composition of the present invention obtained in Test Example 1 against acne bacteria. First, in order to activate Propionibacterium acnes, which is the cause of acne, it was pre-cultured in BHI liquid medium (Brain-Heart Infusion Broth; 3.7%) for 48 hours. The bacterial culture solution thus prepared was spread on BHI solid medium (Brain-Heart Infusion Broth; 3.7%; agar1.5%) by 0.1 ml and dried. The composition obtained in Test Example 1 was diluted to 12% (w/v) in a 95% ethanol aqueous solution, and 50 μl of each was dropped onto a paper disk having a diameter of 8 mm, placed on the solid medium prepared above, and then this was prepared in a 35°C anaerobic culture tank. Incubated for 3 days. The antibacterial activity was evaluated by observing the area to prevent the growth of bacteria around the paper disc and measuring the size of the ring, and the results are shown in Table 4, and it was confirmed that the growth of bacteria was inhibited by looking at Table 4.

Manufacturing example One 2 3 4 Bacterial growth stop ring (㎜) 19 23 24 27

As described above, the illite eluate according to the present invention exhibited the effect of reducing sebum cell differentiation and sebum accumulation, reducing skin shine due to excessive sebum secretion, and thus preventing acne and skin pruning.

Accordingly, it can be seen that cosmetic compositions such as lotions, creams, emulsions, packs, and powders containing the illite eluate have excellent cosmetic effects such as improving sebum secretion, preventing and improving acne, hair growth, and soothing skin.

Claims (7)

Containing an eluent obtained by condensing purified water vapor generated by placing illite, pulverized dry prickly pear powder and purified water in a heating furnace, or condensing purified water vapor through illite and pulverized dried prickly pear powder and condensing the vapor. A cosmetic composition, characterized in that it exhibits sebum secretion inhibitory effect, acne improvement effect, or skin soothing effect.
The method of claim 1,
A cosmetic composition, characterized in that it exhibits sebum secretion inhibitory effect, acne improvement effect, or skin soothing effect, characterized in that it further comprises pulverized basalt when the illite and the pulverized dry sprout sprout powder are eluted with purified water.
The method of claim 1,
The illite eluate is 150 g of illite mineral pulverized to have a diameter of 0.3 mm, 150 g of dried wild sprouts pulverized to the same particle size as the illite mineral, and 500 g of purified water in a heating furnace, 2 atm, 200 A cosmetic composition, characterized in that obtained by heating for 20 minutes under conditions of °C to generate purified water vapor and condensing the generated vapor.
The method of claim 2,
The illite eluate is 150 g of illite mineral pulverized to have a diameter of 0.3 mm, 50 g of dried crypt sprouts crushed to the same particle size as the illite mineral, and basalt crushed to the same particle size as the illite mineral 100g and 500g of purified water are put in a heating furnace, heated for 20 minutes under a condition of 2 atmospheres and 200°C to generate purified water vapor, and a cosmetic composition obtained by condensing the generated vapor.

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