KR102173580B1 - Preparation method of encapsulated seaweed spores - Google Patents

Abstract

The present invention relates to a method for producing a seaweed spore coating bead having an antibacterial property and avoidance of living organisms, improving the growth rate and survival rate of seaweed spores.

Description

Preparation method of encapsulated seaweed spores {Preparation method of encapsulated seaweed spores}

The present invention relates to a method for producing seaweed spore coating beads, and more specifically, by coating seaweed spores with alginic acid containing silica particles, increasing the growth rate and survival rate of seaweed spores, and antimicrobial properties against microorganisms and against breakfast organisms. It relates to a method for producing seaweed spore coating beads with improved avoidability.

The world is experiencing climate change such as rising water temperature and rising sea levels due to global warming. Due to climate change, seaweeds such as green seaweed, seaweed, and Ecklonia disappear from the coasts of Korea, and the sea desertification phenomenon, which is a desertification of the sea, is spreading, in which the calcareous algae, which are composed of calcareous, cover the bedrock and turn white. The coastal ecosystem is rapidly deteriorating and marine resources are decreasing with the spread of seaweed greening.

Seaweeds are an important primary producer of marine ecosystems, and marine forests made up of large seaweeds are an important resource because they provide habitat for many marine organisms and absorb carbon dioxide from the atmosphere. Recently, a sea forest creation project is being implemented in Korea to restore marine ecosystems destroyed by the seaweed greening phenomenon.

The technology generally used for the sea forest creation project is a technology using artificial reefs, and in addition, various technologies such as spore bag, seedling transplantation method, and fertilization agent are used. However, the sea forest creation project technology currently used has a problem of environmental pollution when overused, and has limitations such as cost problems, difficulty in management, and time and location restrictions.

Seaweeds have different spore release times and life cycles for each species, and for efficient sea forest formation, the released spores must be encapsulated to increase the growth rate and survival rate compared to the existing seaweed spores so that they can survive in the harsh sea environment.

Alginic acid is usually used as a material for coating seaweed spores, but if only alginic acid is used in the sea, it is no longer due to the formation of fungi and microorganisms that inhibit the growth and survival of seaweed spores, and sea urchins and starfish, which are breakfast organisms that eat alginic acid. It becomes difficult to grow into a large frond.

Alginic acid is a polysaccharide that makes up the cell wall of brown algae, and is known to be broken down by bacteria in sea urchins and starfish, which are breakfast organisms. In order to prevent the intake of these breakfast organisms, a film made of alleles that they avoid is required. Therefore, in order to improve the growth rate and survival rate of alginic acid, which is a material for coating the existing seaweed spores, it is a coating material that is cheap and easy to obtain in alginic acid, which increases the growth rate and survival rate of seaweed spores, and improves antimicrobial activity against microorganisms and avoidance of breakfast organisms. Skills that can be made are required.

An object of the present invention is to improve the growth rate and survival rate of seaweed spores, and to provide a method for producing seaweed spore coating beads with improved antimicrobial activity against microorganisms and avoidance against breakfast organisms.

Another object of the present invention is to provide a seaweed spore cultivation method or seaweed cultivation method using the seaweed spore coating bead.

For efficient sea forest formation, the released spores must be encapsulated to increase the growth rate and survival rate compared to the existing seaweed spores so that they can survive in the sea environment better. It is an object of the present invention to improve the growth rate and survival rate of seaweed spores, and to improve the antimicrobial activity against microorganisms and avoidance against breakfast organisms.

Another object of the present invention is to increase the growth and survival of spores by making seaweed spores beaded without changing the sea substrate itself, thereby increasing the growth and survival of spores, and improving antibacterial properties against microorganisms and avoidance against breakfast organisms. It is to provide a method for encapsulating spores.

First, the present invention,

a) preparing a coating agent by mixing seaweed spores in an aqueous alginic acid solution;

b) preparing a curing agent by dissolving at least one selected from calcium chloride, calcium propionate, calcium benzoate, calcium sorbate, calcium salicylate, calcium acetate, and calcium levulinate in seawater;

c) forming spore coating beads by dropping the coating agent on the curing agent;

d) washing the spore-coated beads with distilled water; it provides a method for producing seaweed spore-coated beads comprising.

According to the present invention, a method for producing a coating bead of seaweed spores includes a) preparing a coating agent by mixing seaweed spores with an aqueous alginic acid solution.

According to the present invention, the alginic acid aqueous solution may contain 2 to 6% by weight of alginic acid, preferably 3 to 5% by weight, and more preferably 4% by weight. When contained in the above range, it was confirmed through an experiment that it is effective in improving the growth rate and survival rate of seaweed spores.

The alginic acid is a natural polysaccharide that is a cell wall component of brown algae, and is a copolymer composed of two parts of guluronic acid and mannuronic acid. It is represented by the following Chemical Formula 1, the degree of polymerization is 80, and the molecular weight is 15,000 to 240,000.

[Formula 1]

According to the present invention, the seaweed spores are preferably contained in a concentration of 10 ³ ~ 10 ⁷ /ml.

In the present invention, the seaweed may be selected from the group consisting of green laver, seaweed, seaweed, kelp, tot, silmal, agar, mazaban, and hearing, but is not limited thereto, and any type of seaweed classified as seaweed Can be used.

The coating agent may coat seaweed spores, thereby increasing the growth rate and survival rate of seaweed spores.

According to the present invention, the alginic acid aqueous solution may further comprise 0.01 to 1.5 parts by weight of at least one nano-silica particle selected from hydrophilic nano-silica particles, hydrophobic nano-silica particles, or combinations thereof based on 100 parts by weight of alginic acid. have.

If the content of the nanosilica particles is more than the above range, there is a problem that the beads are easily broken, and if it is less than the above range, the effect of improving the growth rate and survival rate of seaweed spores is insignificant compared to the case of using an aqueous solution containing only alginic acid. Therefore, it is preferable to be included in the above range.

Hydrophilic or hydrophobic nanosilica is easy to obtain and is inexpensive. Seaweed spores need external stimulation to attach and germinate and grow. Existing studies related to this have been found to be affected by the physical properties of the surface to which stem cells adhere. In addition, the recognizable area of seaweed spores is in micrometers, and if a nano-sized silica material that is smaller than that is mixed with alginic acid, which is a conventional coating material, and beaded, it does not change the substrate itself in the sea and gives an external stimulus easily. It can have a positive effect on growth.

The nanosilica particles are nanometer-sized silica particles, and are actively used in the bio field due to their well-ordered pore structure and delivery of drugs and enzymes. In the present invention, the nanosilica particles are used as an inorganic substance that induces recognition of the physical properties of seaweed spores.

Nano-silica particles have the advantage that silica having various physical properties can be easily obtained through a simple chemical reaction. In the present invention, hydrophilic silica having the characteristics of conventional silica and hydrophobic silica made by fixing the hydrophilic group of silica can be used alone or in combination. When using an alginic acid aqueous solution containing such nanosilica particles, only an alginic acid aqueous solution is used. Compared to the case, the growth rate and survival rate of seaweed spores are improved.

According to the present invention, if the alginic acid aqueous solution contains at least one selected from hydrophilic nano-silica particles, hydrophobic nano-silica particles, or combinations thereof, the curing agent is calcium chloride, calcium propionate, calcium benzoate, calcium sorbate, calcium salicylate. , Calcium acetate and calcium levulinate may be dissolved in one or more, but preferably calcium chloride. Calcium chloride is economical because it is easy to obtain and inexpensive.

According to the present invention, the hydrophilic nanosilica particles are compounds represented by the following formula (2), and the hydrophobic nanosilica particles may include a compound represented by the following formula (3).

[Formula 2]

[Formula 3]

According to the present invention, the compound represented by Formula 3 can be prepared by reacting the compound represented by Formula 2 with APTMS ((3-aminopropyl) trimethylsilane).

According to the present invention, the method for producing seaweed spore coating beads b) prepares a curing agent by dissolving at least one selected from calcium chloride, calcium propionate, calcium benzoate, calcium sorbate, calcium salicylate, calcium acetate and calcium levulinate in seawater. It includes the step of.

According to the present invention, the curing agent may be contained in a concentration of 2.5 to 3.5% by weight. When the content of the curing agent exceeds the above range, the efficiency decreases because it is not completely dissolved in seawater, and when the content of the curing agent is less than the above range, the viscosity decreases during formation of the beads, and thus the beads are easily broken. According to the present invention, the curing agent may preferably be contained in 3% by weight.

According to the present invention, the curing agent may be prepared by dissolving at least one selected from calcium chloride, calcium propionate, calcium benzoate, calcium sorbate, calcium salicylate, calcium acetate and calcium levulinate in seawater. Calcium chloride is a traditional hardener, and calcium propionate, calcium benzoate (benzoic acid), calcium sorbate, calcium salicylate, calcium acetate, and levulinic acid are antimicrobial organic acids that are actively used as preservatives in processed foods to inhibit the growth of fungi and many microorganisms. . According to the present invention, calcium propionate, calcium benzoate (benzoic acid), calcium sorbate, calcium salicylate, calcium acetate, levulinic acid, or a combination thereof do not affect the growth of seaweed spores, but interfere with growth and survival. It is suitable as a material to suppress and microbes. Each of these structures is shown in the following Chemical Formulas 4 to 9.

[Formula 4]

[Formula 5]

[Formula 6]

[Formula 7]

[Formula 8]

[Formula 9]

According to the present invention, a method of producing a seaweed spore coating bead includes the step of c) forming a spore coating bead by dropping the coating agent in the curing agent.

Preferably, the step of forming spore coating beads by dropping the coating agent on the curing agent is preferably performed for 30 minutes to 1 hour. After dropping, only the surface of the bead is cured within 1 minute, only about half of the bead is cured within 15 minutes, and there is a problem that the inside is not cured, and curing may proceed to the inside of the bead after about 30 minutes. According to the present invention, in step c), in order to prevent the formed beads from sticking together, it is preferable to drop the coating agent while stirring the curing agent.

Alginic acid can be broken down by bacteria present in the breakfast organisms such as sea urchins and starfish. Accordingly, in the present invention, after step c), c1) the formed spore coating bead is added to a mixed aqueous solution of a trivalent iron ion; and a polyphenol compound, lignin, or both; and stirred at pH 7.5 to 8.5 to form a coating film. It may further include the step of.

The coating of the polyphenol compound or lignin is preferable because it can inhibit or kill the proliferation of fungi, bacteria, and viruses existing in the breakfast organisms or in the aquatic environment while preventing the breakfast organisms from ingesting seaweed spores.

According to the present invention, the polyphenol compound may be gallic acid, tannic acid, or both.

Antimicrobial organic acid according to the present invention; The polyphenolic compound or/and the lignin-introduced alginic acid film does not decompose alginic acid for a long time in an aquatic environment, and increases resistance to external stresses, and against marine microorganisms competing for nutrients and space with algae spores in an aquatic environment. Having a raincoat can improve the growth rate and survival rate of seaweed spores.

According to the present invention, the method for producing algal spore coating beads includes: d) washing the spore coating beads with distilled water.

Washing is to remove the curing agent from the spore beads or the compound used in the coating.

In addition, the present invention provides a method for culturing seaweed spores comprising the step of culturing the seaweed spore coating bead prepared by the above method in a culture medium.

According to the present invention, the culture medium is 0.5 to 1.5 parts by weight of PES buffer, preferably 0.8 to 1.2 parts by weight, more preferably 1 part by weight, based on 100 parts by weight of seawater; And GeO ₂ 0.1 to 1 part by weight, preferably 0.4 to 0.6 part by weight, more preferably 0.5 part by weight.

According to the present invention, the cultivation may be performed by irradiating light with 3000 to 4000 lux at preferably 15 to 23°C, preferably 16 to 21°C. The water temperature is preferable because it is similar to the seawater environment.

For example, using a culture medium comprising 1 part by weight of PES buffer and 0.5 part by weight of GeO _{2 based} on 100 parts by weight of seawater, it may be irradiated with 3000 lux light at 18°C.

According to the present invention, the light cycle during the culture may be a light cycle commonly used in seaweed spore culture, and the light/dark cycle may be 18 hours/6 hours to 6 hours/18 hours, for example, light /The dark cycle can be 12 hours/12 hours.

In addition, the present invention provides a seaweed cultivation method comprising; attaching the seaweed spore coating bead prepared by the above method to a rope or a net foot.

The rope or net foot is a transverse string that becomes a substrate on which spores can develop. Spores can be attached to ropes or net feet and transplanted into the sea, or spores germinated through initial culture and then transplanted into the sea.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of the person skilled in the art using the basic concept of the present invention defined in the following claims are also present. It belongs to the scope of rights of

According to the present invention, not only unencapsulated seaweed spores, but also the growth rate and survival rate of spores are higher than that of alginate beads used previously, and have antibacterial properties and avoidance of breakfast organisms. In addition, it is economical because time and cost are greatly reduced because it can be easily made in the process of manufacturing beads without the need for further chemical treatment after coating with an alginic acid compound.

1 is a diagram schematically showing a process of manufacturing a coating bead using nanosilica particles for improving the growth rate and survival rate of seaweed spores according to the present invention.
2 is a diagram schematically showing the chemical structures of hydrophilic silica and hydrophobic silica according to the present invention.
3 is a diagram showing the growth rate of seaweed spores according to the conditions according to the present invention.
4 is a diagram showing the survival rate of seaweed spores according to the conditions according to the present invention.
Figure 5 is a schematic view showing a method for testing the biological avoidance of the seaweed spore coating bead prepared according to the present invention.

Seaweeds are an important primary producer of marine ecosystems, and marine forests made up of large seaweeds are an important resource because they provide habitat for many marine organisms and absorb carbon dioxide from the atmosphere. Recently, a sea forest creation project is being implemented in Korea to restore marine ecosystems destroyed by the seaweed greening phenomenon.

The technology generally used for the sea forest creation project is a technology using artificial reefs, and in addition, various technologies such as spore bag, seedling transplantation method, and fertilization agent are used. However, the sea forest creation project technology currently used has a problem of environmental pollution when overused, and has limitations such as cost problems, difficulty in management, and time and location restrictions.

For efficient sea forest formation, the released spores must be encapsulated to increase the growth rate and survival rate compared to the existing seaweed spores so that they can survive in the sea environment better. When only alginic acid, which is the material for encapsulating seaweed spores, is actually put into the sea, it is difficult to grow into large fronds anymore due to the formation of fungi and microorganisms that inhibit the growth and survival of seaweed spores, and sea urchins and starfish, which are breakfast organisms that eat alginic acid. Lose.

In order to eliminate mold and microbial formation, and for improved growth rate and survival rate, if alginic acid is not decomposed for a long time in the sea environment by adding antibacterial organic acid or nano silica particles to alginic acid, which is widely used as a coating material for seaweed spores. Thus, a novel type of coating bead with increased resistance to external stress was prepared.

Among various seaweeds as seaweed, the experiment was conducted using green seaweed that is easy to obtain from the coast of Korea and has a relatively simple culture and life history.

Example One.

Green spores Bead Produce

A coating agent was prepared by mixing alginic acid with 1% by weight of hydrophilic nanosilica particles of Formula 2 and dissolving in seawater. Next, green spores were added to the coating agent, which is a mixed solution of alginic acid and nano silica particles. At this time, the green spores were contained in a concentration of 10 ³ ~ 10 ⁷ /ml.

CaCl ₂ was dissolved in seawater to prepare a curing agent. The optimum concentration of the curing agent solution is 3%. While stirring the curing agent, the coating agent containing the green spores was added dropwise to form spore beads for 30 minutes. The formed spore beads were washed with distilled water, and CaCl ₂ on the surface of the spore beads was removed.

culture

Next, it was placed in a culture solution containing PES and GeO ₂ in seawater. PES, a medium of green spores, was diluted 100:1 with seawater, and GeO ₂ was diluted 200:1 with seawater to prevent the propagation of diatoms, the most abundant phytoplankton in the ocean, to complete the medium. After the seaweed spore beads were added to the culture medium, the spore beads contained in the culture medium were put in an incubator in which the growth conditions of green spores were adjusted to 18°C, 12L:12D, and 3000 lux, and observed.

Example 2.

Except for using the hydrophobic nanosilica particles of Formula 3 instead of the hydrophilic nanosilica particles of Formula 2, spore beads were formed by the method of Example 1, and observed while culturing.

Example 3.

Green spores Bead Produce

A coating agent was prepared by mixing so as to contain green spores at a concentration of 10 ³ to 10 ⁷ pieces/ml in 4% by weight alginic acid aqueous solution.

A curing agent was prepared by dissolving calcium propionate in seawater. The optimum concentration of the curing agent solution is 3%. While stirring the curing agent, the coating agent containing the green spores was added dropwise to form spore beads for 30 minutes. The formed spore beads were washed with distilled water.

culture

Next, it is placed in a culture solution containing PES and GeO ₂ in seawater. PES, a medium of green spores, was diluted 100:1 with seawater, and GeO ₂ was diluted 200:1 with seawater to prevent the propagation of diatoms, the most abundant phytoplankton in the ocean, to complete the medium. After the seaweed spore beads were added to the culture medium, the spore beads contained in the culture medium were put in an incubator in which the growth conditions of green spores were adjusted to 18°C, 12L:12D, and 3000 lux, and observed.

Example 4.

Except that calcium benzoate was used instead of calcium propionate, spore beads were prepared by the method of Example 3, and observed while culturing.

Example 5.

Except that calcium sorbate was used instead of calcium propionate, spore beads were prepared by the method of Example 3, cultured, and observed.

Example 6.

Except that calcium salicylate was used instead of calcium propionate, spore beads were prepared by the method of Example 3, cultured, and observed.

Example 7.

Spore beads were prepared and cultured according to the method of Example 3, except that calcium acetate was used instead of calcium propionate.

Example 8.

Except that calcium levulinate was used instead of calcium propionate, spore beads were prepared by the method of Example 3, cultured, and observed.

Example 9.

Except that calcium chloride was used instead of calcium propionate, spore beads were prepared by the method of Example 3, and observed while culturing.

Example 10.

Green spore beads were prepared by the method of Example 1. Next, 100 mg of tertiary iron ions were added to 1 L of distilled water, followed by stirring, and tannic acid powder (400 mg/L) in distilled water was added, followed by further stirring until dark purple. The spore beads prepared above were added thereto, and the color change was observed by adjusting the pH to 8 with an aqueous sodium hydroxide solution.

The prepared spore beads coated with tannic acid were washed with distilled water, and iron ions adhering to the surface of the spore beads were removed.

Spore beads coated with tannic acid were added to a culture solution containing PES and GeO ₂ in seawater. PES, a medium of green spores, was diluted 100:1 with seawater, and GeO ₂ was diluted 200:1 with seawater to prevent the propagation of diatoms, the most abundant phytoplankton in the ocean, to complete the medium. After the seaweed spore beads were added to the culture medium, the spore beads contained in the culture medium were put in an incubator in which the growth conditions of green spores were adjusted to 18°C, 12L:12D, and 3000 lux, and observed.

Example 11.

The green spore beads prepared by the method of Example 2 were coated with tannic acid by the method of Example 10, and observed while incubating.

Example 12.

The green spore beads prepared by the method of Example 3 were coated with tannic acid by the method of Example 10, followed by culture and observation.

Example 13.

The green spore beads prepared by the method of Example 4 were coated with tannic acid by the method of Example 10, and observed while culturing.

Example 14.

The green spore beads prepared by the method of Example 5 were coated with tannic acid by the method of Example 10, and observed while incubating.

Example 15.

The green spore beads prepared by the method of Example 6 were coated with tannic acid by the method of Example 10, and observed while incubating.

Example 16.

The green spore beads prepared by the method of Example 7 were coated with tannic acid by the method of Example 10, and observed while incubating.

Example 17.

The green spore beads prepared by the method of Example 8 were coated with tannic acid by the method of Example 10, and observed while incubating.

Example 18.

The green spore beads prepared by the method of Example 9 were coated with tannic acid by the method of Example 10, and observed while incubating.

Example 19.

The green spore beads prepared by the method of Example 9 were coated with tannic acid by the method of Example 10, and observed while incubating.

Comparative example One.

Unencapsulated green spores were observed while culturing.

Comparative example 2.

Alginic acid was dissolved in seawater to prepare a coating agent, and the green spores were coated by the method of Example 1 using this, followed by culture and observation.

Test example 1. Analysis of seaweed spore growth rate

The growth rate of green spores according to each condition in Examples 1 and 2 and Comparative Examples 1 and 2 was analyzed. The growth rate of green spores is shown in FIG. 3 as the chlorophyll a value on the 20th day of culture.

As shown in FIG. 3, the green spores of Example 1 coated with hydrophilic silicon had a growth rate of about 6.3% increased compared to Comparative Example 1, and about 1.3% increased compared to Comparative Example 2. In addition, the green spores of Example 2 coated with hydrophobic silicon were increased by about 11% compared to Comparative Example 1 and increased by about 2.3% compared to Comparative Example 2.

Test example 2. Seaweed spore survival rate analysis

The survival rate of green spores according to each condition in Examples 1 and 2 and Comparative Examples 1 and 2 was analyzed. The survival rate of green spores is shown in FIG. 4 as the germination rate on the 3rd day of culture.

As shown in FIG. 4, the green spores of Example 1 coated with hydrophilic silicon had an increase in survival rate of about 7.8% compared to Comparative Example 1 and an increase of about 1.6% compared to Comparative Example 2. In addition, the green spores of Example 2 coated with hydrophobic silicon were increased by about 10% compared to Comparative Example 1 and about 2.2% compared to Comparative Example 2.

Test example 3. Evasion test for breakfast creatures

Using the blue spore coating beads of Comparative Example 2 (positive control) and the green spore coating beads of Example 19 (experimental group), an evasion test for breakfast organisms was performed. Sea urchin was used as a breakfast creature.

First, the green spore-coated beads of Comparative Example 2 and Examples 12 to 19 were implanted on a transverse line (a rope for seaweed spore growth).

Test example 3.1

A similar marine environment was created in the tank, and food (green) was placed at each end of the tank so that sea urchins could feed, and the sea urchin was placed in the center. Next, between the sea urchin and the food, a transverse line with the previously prepared green spore coating bead was placed so that the sea urchin crosses the transverse line to move to the green sea, and this is schematically illustrated in FIG. 5 (left: control transverse, right: experimental group transverse). . All experiments were repeated 100 times.

Test example 3.2

Experiments were carried out in the same environment as in Test Example 3.1, but no food was placed at both ends of the inside of the tank.

The movement of the sea urchin was observed, and it is shown in Table 1 below.

division
Sea urchin movement (number) Example 19 Comparative Example 2 Not moving Test Example 3.1 4 94 2 Test Example 3.2 10 60 30

n=100

As shown in Table 1, the sea urchin was moved 98 times to feed under the condition of the food, but only 4 times that the green spore-coated beads of Example 19 crossed the transgenic transverse line, and 94 times were comparative examples. The green spore coating bead of 2 crossed the transduced transducer.

On the other hand, under the condition of no food, the sea urchin did not move 30 times, 10 times crossed the transverse line on which the blue spore coat beads of Example 19 were grown, and 60 times the blue spore coat beads of Comparative Example 2 were grown. I crossed the string.

These results demonstrate that when the blue spore coat beads are further coated with a polyphenol compound or lignin, the avoidance of breakfast organisms is increased.

In a further experiment, a result of reduced sea urchin access was obtained even in the transverse lines in which the green spore coat beads of Examples 13 to 18 were formed.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention defined in the following claims are also present. It belongs to the scope of rights of

Claims (15)

a) preparing a coating agent by mixing seaweed spores in an aqueous alginic acid solution;
b) preparing a curing agent by dissolving at least one selected from calcium chloride, calcium propionate, calcium benzoate, calcium sorbate, calcium salicylate, calcium acetate, and calcium levulinate in seawater;
c) forming spore coating beads by dropping the coating agent on the curing agent;
d) washing the spore-coated beads with distilled water; including,
The alginic acid aqueous solution further comprises 0.01 to 1.5 parts by weight of at least one selected from hydrophilic nano-silica particles, hydrophobic nano-silica particles, or combinations thereof based on 100 parts by weight of alginic acid, seaweed spore coating bead production method. The method of claim 1,
The alginic acid aqueous solution is that alginic acid is contained in an amount of 2 to 6% by weight, seaweed spore coating bead production method. delete The method of claim 1,
The seaweed spores are contained in a concentration of 10 ³ to 10 ⁷ /ml, seaweed spore coating bead production method. The method of claim 1,
The curing agent is contained in a concentration of 2.5 to 3.5% by weight, seaweed spore coating bead production method. The method of claim 1,
The curing agent is contained in a concentration of 3% by weight, seaweed spore coating bead production method. The method of claim 1,
The hydrophilic nanosilica particle is a compound represented by the following formula (2), seaweed spore coating bead production method:
[Formula 2]

The method of claim 1,
The hydrophobic nanosilica particle is a compound represented by the following formula (3), seaweed spore coating bead production method:
[Formula 3]

The method of claim 1,
After the step c), c1) the formed spore coating bead is added to a mixed aqueous solution of a trivalent iron ion; and a polyphenol compound, lignin, or both; and stirred at pH 7.5 to 8.5 to form a coating film. That, seaweed spore coating bead manufacturing method. The method of claim 9,
The polyphenol compound is gallic acid, tannic acid, or both, seaweed spore coating bead production method. The method of claim 1,
The seaweed is selected from the group consisting of green onion, laver, seaweed, kelp, tot, sylmal, agar, mazaban and auditory, seaweed spore coating bead production method. A method of culturing seaweed spores comprising the step of culturing the seaweed spore coating beads prepared according to the production method of any one of claims 1 to 2 and 4 to 11 in a culture medium. The method of claim 12,
The culture medium will contain 0.5 to 1.5 parts by weight of PES buffer and 0.1 to 1 parts by weight of GeO _{2 based} on 100 parts by weight of seawater, seaweed spore culture method. The method of claim 12,
The cultivation is carried out by irradiating 3000 to 4000 lux light at 15 to 23 ℃, seaweed spore culture method. Claims 1 to 2 and the step of attaching the seaweed spore coating bead prepared according to any one of the manufacturing method of claim 1 to the rope or net feet; seaweed farming method comprising a.

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