KR102143829B1 - Cosmetic composition for improving skin moisturing and acne comprising cross-linked hyaluronic acid and lupeol - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin moisturizing and acne improvement, comprising cross-linked hyaluronic acid and lupeol as active ingredients. The hyaluronic acid in the cross-linked hyaluronic acid promotes absorption of lupeol, and thus the skin moisturizing and acne improvement activity is improved. In addition, the cosmetic composition for skin moisturizing and acne improvement has an antibacterial activity by forming a film on skin.

Description

A cosmetic composition for improving skin moisturing and acne comprising cross-linked hyaluronic acid and lupeol}

The present invention relates to a cosmetic composition for skin moisturizing and acne improvement comprising cross-linked hyaluronic acid and lupeol, and more particularly, hyaluronic acid in cross-linked hyaluronic acid absorbs lupeol It relates to a cosmetic composition for skin moisturizing and acne improvement having antibacterial activity by forming a film on the skin, as well as improving skin moisturizing and acne improvement activity by promoting it.

Hyaluronic acid is one of complex polysaccharides composed of amino acids and uronic acid, and is a polymer compound composed of N-acetylglucosamine and glucuronic acid.

Hyaluronic acid since it was first isolated from the fluid that fills the eye of an animal, is known as being a lot like the placenta of animals, lubrication of joints, pleural fluid, skin, combs of roosters, Streptococcus spp of Streptococcus equi, Streptococcus zooepidemecus It is also produced in the back.

Because of its excellent biocompatibility and high viscoelasticity in solution, hyaluronic acid is widely used not only for cosmetic applications such as cosmetic additives, but also for various pharmaceutical applications such as ophthalmic surgical aids, joint function improving agents, drug delivery substances and eye drops. have.

However, since hyaluronic acid is easily decomposed in vivo or under conditions such as acid, alkali, etc., its use is limited, so studies to develop structurally stable cross-linked hyaluronic acid are underway. The cross-linked hyaluronic acid has been developed for various uses, such as a cosmetic aid (filler), an anti-adhesion agent after surgery, a drug delivery system, and a cell support (Patent Documents 1 to 3).

However, the prior art uses crosslinked hyaluronic acid as a filler for shaping, an insert for treating wrinkles, and the like, and there is no known example of using it for skin moisturizing.

In addition, a cosmetic composition for relieving acne and suppressing sebum secretion containing lupeol is disclosed (Patent Document 4), but with respect to a cosmetic composition for skin moisturizing and acne improvement comprising crosslinked hyaluronic acid and lupeol It is not known.

Republic of Korea Patent Publication No. 10-2013-0123080 Korean Patent Application Publication No. 10-2017-0025778 Korean Patent Application Publication No. 10-2017-0090965 Republic of Korea Patent Publication No. 10-2018-0134084

An object of the present invention is that hyaluronic acid in crosslinked hyaluronic acid promotes skin absorption of lupeol, thereby improving skin moisturizing and acne improvement activity, as well as skin moisturizing and acne improvement having antibacterial activity by forming a film on the skin. It is to provide a cosmetic composition.

The present invention provides a cosmetic composition for skin moisturizing and acne improvement, comprising cross-linked hyaluronic acid and lupeol as active ingredients in order to achieve the above object.

In addition, the present invention provides a cosmetic composition for skin moisturizing and acne improvement comprising 0.001 to 10% by weight of the crosslinked hyaluronic acid and 0.001 to 5% by weight of the lupeol relative to the total weight of the composition.

In addition, the present invention provides a cosmetic composition for skin moisturizing and acne improvement further comprising aloe extract, centella asiatica extract and polydioxyribonucleotide (PDRN) in addition to the cross-linked hyaluronic acid and lupeol. .

In addition, in the present invention, the crosslinked hyaluronic acid is 1,4-butanediol diglycidyl ether, ethylene glycol diglycidyl ether, 1,6-hexanediol diglycidyl ether, propylene glycol diglycidyl ether , Polypropylene glycol diglycidyl ether, polytetramethyl glycol diglycidyl ether, neopentyl glycol diglycidyl ether, polyglycerol polyglycidyl ether, diglycerol polyglycidyl ether, glycerol polyglycidyl Ether, trimethylpropane polyglycidyl ether, 1,2-(bis(2,3-epoxypropoxy)ethylene, pentaerythritol polyglycidyl ether, and at least one selected from the group consisting of sorbitol polyglycidyl ether It provides a cosmetic composition for skin moisturizing and acne improvement cross-linked with an epoxide crosslinking agent.

In addition, the present invention provides a cosmetic composition for skin moisturizing and acne improvement in which the molecular weight of the cross-linked hyaluronic acid is 100,000 to 1,000,000 Da.

In addition, the present invention provides a cosmetic composition for skin moisturizing and acne improvement in which the lupeol is derived from fan beans.

In addition, the present invention is based on the total weight of the composition, cross-linked hyaluronic acid 0.001 to 10% by weight, lupeol 0.001 to 5% by weight, aloe extract 0.001 to 5% by weight, centella extract 0.001 to 5% by weight, and polydeoxyribonucleotide (PDRN) It provides a cosmetic composition for skin moisturizing and acne improvement containing 0.0001 to 5% by weight.

In addition, the present invention provides a cosmetic composition for skin moisturizing and acne improvement in which the formulation of the composition is a cream, lotion, toner, emulsion or spray.

In addition, the present invention is white lupine seed extract, quinoa seed extract, oat extract, cactus extract, barley extract, edelweiss extract, lentil extract, ice plant extract, sugar forsythia extract, sea grape extract, horsetail kelp extract, epidermis extract , Burdock seed extract, Angelica extract, Pogongyeong extract, Magnolia flower extract, Licorice extract, Sesin extract, Mint extract, Golden extract, Hwangryeon extract, Hwangbaek extract, Broiler extract, Jujube extract, Japonica extract, Yeongyo extract, Astragalus extract, Elm tree It provides a cosmetic composition for skin moisturizing and acne improvement further comprising a herbal extract selected from the group consisting of extract, Gosam extract, casualty extract, Changija extract, tangerine peel extract, and oil extract.

In addition, the present invention provides a cosmetic composition for skin moisturizing and acne improvement comprising 0.001 to 10% by weight of the herbal extract, based on the total weight of the composition.

According to the present invention, it is possible to provide a cosmetic composition for skin moisturizing and acne improvement having antibacterial activity by forming a film on the skin, as well as improving the skin moisturizing and acne improvement activity by promoting absorption of the cross-linked hyaluronic acid. I can.

1 is a photograph showing a condition after 10 days after applying the cream composition of Example 1 to the face after washing face twice a day in the morning and evening for a 27-year-old woman with complex and sensitive skin type.

The present invention relates to a cosmetic composition for skin moisturizing and acne improvement, comprising cross-linked hyaluronic acid and lupeol as active ingredients.

The crosslinked hyaluronic acid used as an active ingredient of the cosmetic composition of the present invention may be obtained by crosslinking hyaluronic acid using an epoxide crosslinking agent having two or more epoxy reactive groups.

In the present invention, the term "hyaluronic acid" includes all of hyaluronic acid, a hyaluronic acid salt, or a mixture of hyaluronic acid and a hyaluronic acid salt, wherein the hyaluronic acid salt is sodium hyaluronate, potassium hyaluronate, calcium hyaluronate, hyaluronic acid It may be one or more selected from the group consisting of magnesium hydroxide, zinc hyaluronate, cobalt hyaluronate, and tetrabutyl ammonium hyaluronate, preferably sodium hyaluronate, but is not limited thereto.

The crosslinked hyaluronic acid is crosslinked by dissolving hyaluronic acid or hyaluronic acid salt in an alkaline aqueous solution, and then crosslinking reaction by adding an epoxide crosslinking agent to the hyaluronic acid or hyaluronic acid salt solution, and then washing the crosslinked reactant several times to unreacted product. It can be produced by removing.

Crosslinking agents used in the crosslinking reaction include 1,4-butanediol diglycidyl ether, ethylene glycol diglycidyl ether, 1,6-hexanediol diglycidyl ether, propylene glycol diglycidyl ether, and polypropylene. Glycol diglycidyl ether, polytetramethyl glycol diglycidyl ether, neopentyl glycol diglycidyl ether, polyglycerol polyglycidyl ether, diglycerol polyglycidyl ether, glycerol polyglycidyl ether, trimethyl At least one epoxide crosslinking agent selected from the group consisting of propane polyglycidyl ether, 1,2-(bis(2,3-epoxypropoxy)ethylene, pentaerythritol polyglycidyl ether, and sorbitol polyglycidyl ether For example, 1,4-butanediol diglycidyl ether may be used most preferably, but is not limited thereto.

The epoxide crosslinking agent is a crosslinking agent containing two or more epoxy functional groups, and is crosslinked by reacting with an alcohol group of hyaluronic acid to form an ether bond.

The crosslinking density of the crosslinked hyaluronic acid used as an active ingredient of the cosmetic composition of the present invention may be 0.001 to 10%, more preferably 1 to 5%. When the crosslinking density is less than 0.001%, there is a problem in that physical properties are lowered and water absorption is increased, which makes purification difficult. When it exceeds 10%, there is a high possibility that a large amount of crosslinking agent remains.

The cosmetic composition of the present invention may also contain 0.001 to 10% by weight, preferably 0.001 to 5% by weight, and more preferably 0.001 to 2% by weight, based on the total weight of the crosslinked hyaluronic acid. When the cross-linked hyaluronic acid is included in less than 0.001% by weight, the intended skin moisturizing effect cannot be achieved, and when the cross-linked hyaluronic acid is included in excess of 10% by weight, a predetermined or more skin moisturizing effect is achieved. Since it is not increased, there is a problem in that the manufacturing cost is increased.

The molecular weight of the crosslinked hyaluronic acid contained in the cosmetic composition of the present invention may be 100,000 Da or more, preferably 100,000 to 1,000,000 Da, but outside the above range, the effect of delaying the decomposition rate in the skin is not excellent. Or, there is a problem that the moisturizing effect of the skin is relatively weak.

In the present invention, the term "lupeol" is a plant-derived, preferably fan bean-derived, more preferably a bean pod or flower-derived fat-soluble component, which can directly dissolve sebum, , It can sterilize acne bacteria by entering the pores, and it functions to suppress various causes of acne such as sebum production, inflammation, and keratinization that cause acne.

In the cosmetic composition of the present invention, hyaluronic acid contained in the cross-linked hyaluronic acid promotes skin absorption of the lupeol, thereby enhancing skin moisturizing and acne improvement activity, and the cross-linked hyaluronic acid is a coating of the skin. By maintaining the formation, it is possible to prevent the penetration of bacteria and maximize the acne healing effect.

The cosmetic composition of the present invention may contain 0.001 to 5% by weight of the lupeol based on the total weight of the composition. When the amount of lupeol is less than 0.001% by weight, the target acne improvement effect cannot be obtained, and when the amount of lupeol exceeds 10% by weight, the acne improvement effect above a predetermined level is not increased. There is a problem that the cost increases.

In addition to the crosslinked hyaluronic acid and lupeol, the cosmetic composition of the present invention may further include aloe extract, centella asiatica extract, and polydeoxyribonucleotide (PDRN).

The aloe (Aloe) is a plant belonging to the lily family of the monocotyledonous plant, and promotes blood circulation, improves body cell fluid, decomposes toxic substances in the body, and strengthens antibacterial ability, and makes normal cells of the body the same. It functions such as imparting the ability to form cells of a property.

In addition, Centella asiatica is a plant of the family Parsley family, and the extract of the folding screen contains components such as asiaticoside, madecassoside, and asiatic acid, and is known to have excellent cell regeneration effect.

Since the aloe extract or the folding screen extract is sufficient to be extracted and used by a solvent extraction method such as hot water extraction or ethanol known in the art, a detailed description thereof will be omitted.

In addition, the polydeoxyribonucleotide (PDRN) is a substance extracted from testis such as salmon and trout, and promotes cell regeneration to quickly heal wounds while reducing scar formation and reducing pain. It is widely used under license, and it is also used as a specialized medicine in Korea to treat wounds, ulcers, and to promote tissue regeneration after surgery.

The cosmetic composition of the present invention, based on the total weight of the composition, cross-linked hyaluronic acid 0.001 to 10% by weight, lupeol 0.001 to 5% by weight, aloe extract 0.001 to 5% by weight, centella extract 0.001 to 5% by weight and poly It may contain 0.0001 to 5% by weight of deoxyribonucleotide (PDRN).

The formulation of the cosmetic composition of the present invention may be a cream, lotion, toner, emulsion or spray, but is not limited thereto, and may be modified and used in a formulation known in the art as needed.

The cosmetic composition of the present invention is quinoa seed extract, oat extract, cactus extract, adlay extract, edelweiss extract, lentil extract, ice plant extract, sugar forsythia extract, sea grape extract, horsetail kelp extract, mortar extract, burdock seed Extract, Angelica extract, Pogongyeong extract, Magnolia flower extract, Licorice extract, Sesin extract, Mint extract, Golden extract, Hwangryeon extract, Hwangbaek extract, Broiler extract, Jujube extract, Japonica extract, Yeongyo extract, Astragalus extract, Elm extract, Sophora ginseng It may further include a herbal extract selected from the group consisting of extract, casualty extract, changja extract, tangerine peel extract, and oil extract, but is not limited thereto.

The herbal extracts contained in the cosmetic composition of the present invention are preferably quinoa seed extract, oat extract, cactus extract, adlay extract, edelweiss extract, lentil extract, ice plant extract, sugar forsythia extract, sea grape extract and hose It may be selected from the group consisting of tail kelp extract.

The cosmetic composition of the present invention may further include 0.001 to 10% by weight of the herbal extract based on the total weight of the composition, but the content of the herbal extract may be adjusted as necessary.

The herbal extracts that can be further included in the cosmetic composition of the present invention include herbal substances such as quinoa seed extract, oat extract, etc., added to purified water in an amount of 5 to 20 times the weight of the herbal medicine, and the volume of the first purified water at 50 to 120°C. After extraction so as to be 5 to 50%, the obtained by filtration may be used, but is not limited thereto.

The herbal extract can purify the skin and form moisture to moisturize the skin and protect the skin in any skin environment.

The cosmetic composition of the present invention may further include fatty substances, organic solvents, solubilizers, thickening and gelling agents, softening agents, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or Nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in cosmetics It may contain an adjuvant commonly used in the field of cosmetics or dermatology such as, but is not limited thereto.

In addition, the above ingredients may be introduced in an amount generally used in the field of dermatology.

In particular, when the cosmetic composition according to the present invention is a cream formulation, as a carrier component, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. May be used, but is not limited thereto.

In addition, when the cosmetic composition according to the present invention is a spray formulation, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as carrier components, and additionally, chlorofluorohydrocarbon, propane/ It may include a propellant such as butane or dimethyl ether, but is not limited thereto.

Hereinafter, the content of the present invention will be described in detail through examples. However, these examples are for describing the present invention in more detail, and the scope of the present invention is not limited thereto.

<Preparation Example> Preparation of crosslinked hyaluronic acid

Hyaluronic acid was dissolved in 100 ml of 0.25N NaOH aqueous solution to prepare 18 g of hyaluronic acid solution. 3 g of 1,4-butanediol diglycidyl ether (BDDE) was added to the hyaluronic acid solution, stirred at 35° C. for 48 hours, and then crystallized with ethanol to obtain 18 g of crosslinked hyaluronic acid (crosslinking degree 5%).

At this time, the hyaluronic acid (MW: 120 kDa) was provided by Shideido, Inc., and the 1,4-butanediol diglycidyl ether (MW: 202.25Da) was purchased from Sigma-Aldrich (ST. Louis. MO, USA). I did. In addition, sodium hydroxide (MW: 40 Da) was also purchased from Sigma-Aldrich.

<Experimental Example 1> (Cytotoxicity test)

The cell viability of the crosslinked hyaluronic acid obtained in Preparation Example was measured through MTT assay.

MTT assay is an experimental method that is widely used for cell proliferation and toxicity by measuring the number of living cells.Live cells are water-soluble in the mitochondria, and MTT, a yellow salt, is reduced to water-insoluble blue formazan derivatives by succinate dehydrogenase. It is to use the principle of becoming.

First, HaCaT cells were inoculated into a 6-well plate at a density of 1×10 ⁻⁵ cells/well and incubated at 37° C. for one day. After that, each sample was treated in each well by concentration (1, 10, 20 µg/ml). Then, after incubation at 37°C for 12 hours, the MTT solution was treated, activated for 1 hour, and measured at 450 nm with an ELISA reader. The control was performed with H ₂ O ₂ , and cell viability was shown compared to the control.

The experimental results are shown in Table 1 below, and it can be seen that the crosslinked hyaluronic acid obtained in Preparation Example exhibits superior survival rate compared to the control group.

division 12 hours Control 100 0.2 Manufacturing example 1㎍/ml 123 0.17 10㎍/ml 127 0.15 20㎍/ml 126 0.23

<Example 1> (Preparation of a cosmetic composition containing cross-linked hyaluronic acid and lupeol)

After heating and mixing the oil phase and the aqueous phase at 75° C., respectively, at the composition and content (% by weight) shown in Table 2 below, it was cooled to room temperature to prepare a cosmetic composition in a cream formulation containing crosslinked hyaluronic acid and lupeol.

ingredient Content (% by weight) Crosslinked HA of Preparation Example 0.05 Sodium hyaluronate 0.05 Betaine 2.00 Xanthan gum 0.06 PDRN (sodium DNA) 100ppm 0.01 Hydrolyzed collagen 0.10 Disodium EDTA 0.03 Allantoin 0.30 Ceramide NP 0.10 Adenosine 0.04 Butylene Glycol 5.00 Glyceres-26 3.00 Cetearyl alcohol 1.20 Stearic acid 0.50 Beads wax 0.30 Cetearylolivate, sorbitanolivate 3.00 Polyglyceryl-3methylglucose distearate 1.00 Ascorbyl Tetraisopalmitate 0.20 Blue Tanji Flower Oil 0.05 Ethylhexyl palmitate 4.00 Camellia seed oil 2.00 Argan kernel oil 2.00 Macadamia seed oil 2.00 Jojoba seed oil 2.00 Sunflower seed oil 1.00 Tocopheryl Acetate 0.20 Glyceryl Stearate, PEG-100 Stearate 1.80 Cyclopentasiloxane, cyclohexasiloxane 3.00 Dimethicone 1.00 Polyacrylate-13, Polyisobutene, Polysorbate 20 0.50 Rose essential oil 0.20 White Lupine Seed Extract (Lupeol) 2.00 Quinoa Seed Extract 0.50 Oat extract 0.50 Cactus extract 0.50 Adlay extract 0.50 Edelweiss Extract 0.50 Lentil extract 0.50 Ice Plant Extract 0.50 Sugar forsythia extract 0.50 Sea grape extract 0.50 Horsetail Gelp Extract 0.50 amino acid 2.00 Beta-glucan 2.00 Glyceryl glucoside 1.00 Kelp extract 2.00 Centella asiatica extract 0.25 Aloe extract 0.50 1,2-hexanediol & ethylhexyl glycerin 2.10 Purified water Balance Sum 100

In Table 2, amino acids include glycine, alanine, valine, leucine, isoleucine, phenylalanine, tyrosine tryptophan, lysine, arginine, histinine, aspartic acid, glutamic acid, cysteine, methionine, serine, threonine, and proline.

<Example 2> (Preparation of a cosmetic composition containing crosslinked hyaluronic acid and lupeol)

The oil phase and the aqueous phase were heated and mixed at 75° C., respectively, in the composition and content (% by weight) shown in Table 3 below, and then cooled to room temperature to prepare a cosmetic composition in an emulsion formulation containing crosslinked hyaluronic acid and lupeol.

ingredient Content (% by weight) Crosslinked HA of Preparation Example 0.05 Betaine 1.00 Xanthan gum 0.06 PDRN (sodium DNA) 100ppm 0.01 Disodium EDTA 0.03 Allantoin 0.15 Adenosine 0.04 Glyceres-26 3.00 Butylene Glycol 5.00 Cetearyl alcohol 0.40 Stearic acid 0.50 Polyglyceryl-3methylglucose distearate 1.80 Glyceryl Stearate, PEG-100 Stearate 1.00 Tri-C14-15 alkyl citrate 0.40 Ascorbyl Tetraisopalmitate 0.20 Blue Tanji Flower Oil 0.10 Camellia seed oil 1.50 Argan kernel oil 1.50 Ethylhexyl palmitate 3.00 Hydrogenated Polydecene 3.00 Sunflower seed oil 1.00 Tocopheryl Acetate 0.20 Cyclopentasiloxane, cyclohexasiloxane 3.00 Dimethicone 0.20 Polyacrylate-13, Polyisobutene, Polysorbate 20 0.30 Rose essential oil 0.20 White Lupine Seed Extract (Lupeol) 5.00 Quinoa Seed Extract 0.50 Oat extract 0.50 Cactus extract 0.50 Adlay extract 0.50 Edelweiss Extract 0.50 Lentil extract 0.50 Ice Plant Extract 0.50 Sugar forsythia extract 0.50 Sea grape extract 0.50 Horsetail Gelp Extract 0.50 amino acid 2.00 Beta-glucan 2.00 Glyceryl glucoside 1.00 Kelp extract 2.00 Centella asiatica extract 0.50 Aloe extract 0.50 1,2-hexanediol & ethylhexyl glycerin 2.10 Purified water Balance Sum 100

In Table 3, amino acids include glycine, alanine, valine, leucine, isoleucine, phenylalanine, tyrosine tryptophan, lysine, arginine, histinine, aspartic acid, glutamic acid, cysteine, methionine, serine, threonine, and proline.

<Example 3> (Preparation of a cosmetic composition containing crosslinked hyaluronic acid and lupeol)

After heating and mixing at 75° C. in the composition and content (% by weight) shown in Table 4 below, it was cooled to room temperature to prepare a cosmetic composition of a toner formulation containing crosslinked hyaluronic acid and lupeol.

ingredient Content (% by weight) Crosslinked HA of Preparation Example 0.05 Betaine 1.00 White Lupine Seed Extract (Lupeol) 5.00 Quinoa Seed Extract 0.50 Oat extract 0.50 Cactus extract 0.50 Adlay extract 0.50 Edelweiss Extract 0.50 Lentil extract 0.50 Ice Plant Extract 0.50 Sugar forsythia extract 0.50 Sea grape extract 0.50 Horsetail Gelp Extract 0.50 amino acid 2.00 Beta-glucan 1.00 Glyceryl glucoside 1.00 Kelp extract 1.00 Centella asiatica extract 0.50 Aloe extract 0.50 Xanthan gum 0.03 DisodiumEDTA 0.03 Adenosine 0.04 Glyceres-26 2.00 Ammonium Acryloyl Dimethyl Taurate/VP Copolymer 0.15 Butylene Glycol 5.00 PG-60 Hydrogenated Castor Oil 1.00 Ascorbyl Tetraisopalmitate 0.20 Blue Tanji Flower Oil 0.05 Rose essential oil 0.10 1,2-hexanediol & ethylhexyl glycerin 2.10 Purified water Balance Sum 100

In Table 4, amino acids include glycine, alanine, valine, leucine, isoleucine, phenylalanine, tyrosine tryptophan, lysine, arginine, histinine, aspartic acid, glutamic acid, cysteine, methionine, serine, threonine, and proline.

<Comparative Example 1> (Preparation of a cosmetic composition containing lupeol)

A cosmetic composition in a cream formulation containing lupeol was prepared in the same manner as in Example 1, except that crosslinked hyaluronic acid was not included.

<Comparative Example 2> (Preparation of a cosmetic composition containing lupeol)

A cosmetic composition in an emulsion formulation containing lupeol was prepared in the same manner as in Example 2, except that crosslinked hyaluronic acid was not included.

<Comparative Example 3> (Preparation of a cosmetic composition containing lupeol)

A cosmetic composition of a toner formulation containing lupeol was prepared in the same manner as in Example 3, except that crosslinked hyaluronic acid was not included.

<Example 4> (Preparation of a cosmetic composition containing cross-linked hyaluronic acid, lupeol, and herbal extracts)

As herbal extracts, except that quinoa seed extract, oat extract, cactus extract, adlay extract, edelweiss extract, lentil extract, ice plant extract, sugar forsythia extract, sea grape extract and horsetail kelp extract each further contain 0.5% by weight. Then, in the same manner as in Example 1, a cosmetic composition in a cream formulation including crosslinked hyaluronic acid, lupeol and herbal extracts was prepared.

At this time, the herbal extract is quinoa seed extract, oat extract, cactus extract, adlay extract, edelweiss extract, lentil extract, ice plant extract, sugar forsythia extract, sea grape extract and horsetail kelp extract. , It was added to 10 times the amount of purified water based on the weight of the herbal medicine, extracted so as to be 25% of the original purified water volume at 110°C, and then filtered.

<Example 5> (Preparation of a cosmetic composition containing cross-linked hyaluronic acid, lupeol and herbal extracts)

Excluding the addition of 0.25% by weight of quinoa seed extract, oat extract, cactus extract, adlay extract, edelweiss extract, lentil extract, ice plant extract, sugar forsythia extract, sea grape extract and horsetail kelp extract, respectively, as herbal extracts Then, in the same manner as in Example 2, a cosmetic composition of an emulsion formulation including crosslinked hyaluronic acid, lupeol and herbal extracts was prepared.

At this time, the herbal extract is quinoa seed extract, oat extract, cactus extract, adlay extract, edelweiss extract, lentil extract, ice plant extract, sugar forsythia extract, sea grape extract and horsetail kelp extract. , It was added to 10 times the amount of purified water based on the weight of the herbal medicine, extracted so as to be 25% of the original purified water volume at 110°C, and then filtered.

<Example 6> (Preparation of a cosmetic composition containing cross-linked hyaluronic acid, lupeol, and herbal extracts)

As herbal extracts, except that quinoa seed extract, oat extract, cactus extract, adlay extract, edelweiss extract, lentil extract, ice plant extract, sugar forsythia extract, sea grape extract and horsetail kelp extract each further contain 0.5% by weight. Then, in the same manner as in Example 3, a cosmetic composition of a toner formulation including crosslinked hyaluronic acid, lupeol, and herbal extracts was prepared.

At this time, the herbal extract is quinoa seed extract, oat extract, cactus extract, adlay extract, edelweiss extract, lentil extract, ice plant extract, sugar forsythia extract, sea grape extract and horsetail kelp extract. , It was added to 10 times the amount of purified water based on the weight of the herbal medicine, extracted so as to be 25% of the original purified water volume at 110°C, and then filtered.

<Experimental Example 2> Skin moisturizing effect

For a total of 50 women in their twenties, the cosmetic composition of the cream formulation prepared in Example 1, the cosmetic composition of the cream formulation containing the herbal extract prepared in Example 4, does not contain crosslinked hyaluronic acid The experiment was carried out for each of 10 people using the cream-formed cosmetic composition prepared in Comparative Example 1, and the remaining 10 people were tested as a control group (Comparative Example 4) not using the cosmetic composition.

In the experimental method, the cosmetic composition was applied to each face for 10 days, and the result was compared with that before application, and a comprehensive evaluation was measured for side effects and rejection, such as moistness, tingling, and burning on the face.

In the evaluation, very bad (side effects and discontinuation of the experiment, etc.) were treated as very bad, and the whole, such as very good (10 points), was divided into five stages, and the results are shown in Table 5 (unit: persons). .

division Very good good usually Bad Very bad Sum Average score 10 7.5 5 2.5 0 - - Example 1 7 2 One - - 90 9.0 Example 4 9 One - - - 97.5 9.75 Comparative Example 1 - - 7 3 - 42.5 4.25 Comparative Example 4 - - 5 4 One 35 3.5

As shown in Table 5, the experimental group using the cosmetic composition of Example 1 containing cross-linked hyaluronic acid and lupeol was the experimental group using the cosmetic composition of Comparative Example 1 not containing cross-linked hyaluronic acid and the cosmetic It can be seen that the moisturizing effect is very excellent compared to Comparative Example 4 in which the composition was not used.

On the other hand, it can be seen that the cosmetic composition of Example 4 additionally including an herbal extract has a better moisturizing effect than the cosmetic composition of Example 1 that does not contain it.

<Experimental Example 3> Acne improvement effect

In the morning for each 10 patients with severe acne using the cream-formed cosmetic composition prepared in Example 1 and the cream-formed cosmetic composition prepared in Comparative Example 1 not containing crosslinked hyaluronic acid, It was applied to the entire face for 10 days in the evening to test the effect of improving acne.

For 10 other patients with severe acne, an experiment was conducted as a control group (Comparative Example 4) not using the cosmetic composition. The evaluation was evaluated by dividing the entire effect into five stages, such as very good (5), normal (3), and none (1), and the results are shown in Table 6 (unit: persons).

division Very good good usually Almost none No Sum Average score 5 4 3 2 One - - Example 1 9 One - - - 49 4.9 Comparative Example 1 4 6 - - - 44 4.4 Comparative Example 4 - - - One 9 11 1.1

As shown in Table 6, the cosmetic composition of Example 1 comprising crosslinked hyaluronic acid and lupeol has excellent acne improvement effect compared to the cosmetic composition of Comparative Example 1 that does not contain crosslinked hyaluronic acid. , From this, it can be seen that the hyaluronic acid in the crosslinked hyaluronic acid promotes absorption of lupeol, thereby improving the acne improvement effect.

In addition, it can be seen that the cosmetic composition of Example 1 according to the present invention has very excellent acne improvement effect compared to Comparative Example 4, which is a control group.

On the other hand, Figure 1 is one of the test subjects, targeting a 27-year-old woman with complex and sensitive skin type, after washing her face twice a day in the morning and evening, the cream composition of Example 1 was applied to the face after 10 days. This is a picture showing the condition.

As shown in FIG. 1, the experimenter with redness and acne as a sensitive skin type noticeably improved redness and acne after 10 days, and it can be seen that the troubled skin was also trimmed.

As described above, the present invention has been described with reference to preferred embodiments of the present invention, but those skilled in the art of the present invention can see the present invention within the scope not departing from the spirit and scope of the invention described in the following claims. It will be appreciated that various modifications and changes can be made to the invention.

According to the present invention, a cosmetic composition for skin moisturizing and acne improvement having antibacterial activity by forming a film on the skin as well as improving skin moisturizing and acne improvement activity by promoting absorption of lupeol by hyaluronic acid in cross-linked hyaluronic acid Since it can provide, it can be usefully applied to the technical field to which the present invention belongs.

Claims (9)

As a cosmetic composition for skin moisturizing and acne improvement, comprising cross-linked hyaluronic acid and lupeol as active ingredients,
Including 0.001 to 10% by weight of the crosslinked hyaluronic acid and 0.001 to 5% by weight of the lupeol, based on the total weight of the composition,
Based on the total weight of the composition, the aloe extract 0.001 to 5% by weight, centella extract 0.001 to 5% by weight, and polydeoxyribonucleotide (PDRN) 0.0001 to 5% by weight further comprising,
Based on the total weight of the composition, a herbal extract consisting of quinoa seed extract, oat extract, cactus extract, adlay extract, edelweiss extract, lentil extract, ice plant extract, sugar forsythia extract, sea grape extract and horsetail kelp extract 0.001~ Cosmetic composition for skin moisturizing and acne improvement, characterized in that it further comprises 10% by weight. delete delete The cosmetic composition of claim 1, wherein the crosslinked hyaluronic acid has a molecular weight of 100,000 to 1,000,000 Da. The cosmetic composition for skin moisturizing and acne improvement according to claim 1, wherein the lupeol is derived from fan beans. delete The cosmetic composition of claim 1, wherein the formulation of the composition is a cream, lotion, toner, emulsion or spray. delete delete

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