KR102099521B1 - Method for manufacturing raw material containing S-adenosylmethionine, and external preparation, cosmetic and health food containing liquid raw material containing S-adenosylmethionine - Google Patents

Abstract

Water and brown sugar are added to the culture tank, a warm dissolution process to warm-dissolve, a culture process to inject yeast into the culture tank, a separation process to separate medium and yeast by centrifugation, and lysis of the obtained yeast. It has a lysis treatment step of destroying cells by treatment, and an extraction step of extracting with water under acidic conditions. It was decided to decrease by 15%. Thereby, an S-adenosylmethionine-containing raw material with a high content of S-adenosylmethionine can be obtained. In addition, the obtained liquid S-adenosylmethionine-containing raw material can be blended into cream, gel or liquid external preparations, cosmetics or health foods.

Description

Method for manufacturing raw material containing S-adenosylmethionine, and external preparation, cosmetic and health food containing liquid raw material containing S-adenosylmethionine

The present invention relates to a method for preparing a raw material containing S-adenosyl methionine, an external preparation containing a liquid raw material containing S-adenosyl methionine, cosmetics, and health food.

S-adenosylmethionine was first developed in Europe in 1974 as a treatment for depression. In addition, in the United States, it is particularly popular as a supplement that is effective for arthritis, and it is also believed to be effective for liver disease. In particular, arthritis patients tend to increase year by year, and the demand for health foods to protect joints is increasing.

Here, Patent Document 1, by combining the vinegar derived from brewing such as malt vinegar (も ろ み 청, malt vinegar) and sake sake lees, to obtain a health food containing S-adenosylmethionine in a stable state.

In addition, Patent Document 2 describes that a composition containing S-adenosylmethionine is used as an external preparation for skin such as cosmetics.

Japanese Patent Application Publication No. 2008-11803 International Publication No. 2012/035685

In order to increase the S-adenosylmethionine content of the S-adenosylmethionine-containing raw material blended in external preparations, cosmetics or health foods, it is necessary to increase the S-adenosylmethionine content in the cells of the cultured yeast as much as possible. However, the inventions described in Patent Documents 1 and 2 are not intended to increase the S-adenosylmethionine content in yeast cells.

In addition, conventional S-adenosylmethionine-containing raw materials are powdery and difficult to dissolve in water or oil, making it difficult to blend into creamy, gel-like, or liquid products (such as cosmetic creams). .

Here, an object of the present invention is to provide a method for producing a raw material containing S-adenosylmethionine containing a large amount of S-adenosylmethionine, and an external preparation, cosmetic, and health food in which liquid raw material containing S-adenosylmethionine is blended. .

The method for producing an S-adenosylmethionine-containing raw material of the present invention according to claim 1 is introduced by injecting water and brown sugar into a culture tank, followed by a warm dissolution step of warm dissolving and a warm dissolution step, followed by infusion of yeast into a culture tank. After the cultivation step to be performed, and after the cultivation step, after the separation step to separate the medium and yeast by centrifugation, and after the separation step, the obtained yeast is subjected to lysis treatment to destroy the cells by lysis treatment, and after the lysis treatment step, It is characterized by having an extraction process for extracting with water under acidic conditions, and stopping the culture in the culture step from when the turbidity of the culture medium is switched from rising to falling until turbidity is decreased by 15%. .

The method for producing an S-adenosylmethionine-containing raw material of the present invention according to claim 2 is introduced by injecting water and brown sugar into a culture tank, and heating and dissolving, followed by heating and dissolving, followed by heating and dissolving. After the cultivation step to be performed, and after the cultivation step, after the separation step to separate the medium and yeast by centrifugation, and after the separation step, the obtained yeast is subjected to lysis treatment to destroy the cells by lysis treatment, and after the lysis treatment step, It has an extraction step of performing extraction with water under acidic conditions.

The method for producing the S-adenosylmethionine-containing raw material of the present invention according to claim 3 is introduced by injecting water and brown sugar into a culture tank, and heating and dissolving, followed by heating and dissolving. A culture step to be performed, and a separation step to separate the medium and yeast by centrifugation after the culture step, and the culture stop timing in the culture step is 15% turbidity from when the turbidity of the culture medium is switched from rising to falling. It is characterized in that it is made until it falls.

In the present invention of claim 4, in the method for producing the S-adenosylmethionine-containing raw material according to claim 3, the yeast is cheonju yeast, the temperature in the heating dissolution step is 20 ° C or more and 35 ° C or less, and It is characterized in that the temperature is 20 ° C or more and 35 ° C or less, the concentration of brown sugar is 6% or more and 10% or less by weight, and the initial pH of the medium is 3 or more and 5 or less.

The present invention according to claim 5 is characterized in that it has a drying step of obtaining a dry powder by freeze-drying the yeast after the separation step in the method for producing an S-adenosylmethionine-containing raw material according to claim 3 or 4 Is done.

The external preparation of the present invention according to claim 6 is characterized in that it comprises an extract liquid raw material of yeasts containing S-adenosylmethionine which has been subjected to lysis treatment.

The cosmetic product of the present invention described in claim 7 is characterized in that it comprises an extract liquid raw material of yeast containing S-adenosylmethionine which has been lysed.

The health food of the present invention described in claim 8 is characterized in that it comprises an extract liquid raw material of yeast containing S-adenosylmethionine treated with lysis.

ADVANTAGE OF THE INVENTION According to this invention, the manufacturing method of the raw material containing S-adenosylmethionine with a high content of S-adenosylmethionine, the external preparation, cosmetics, and health food compounded with the liquid raw material containing S-adenosylmethionine can be provided.

1 is a view showing the test results of different culture conditions in the culture step.
Fig. 2 is a diagram showing the transition of pain in the knee joint when an external cream containing S-adenosylmethionine-containing liquid raw material is used.
It is a figure which shows the result of the functional evaluation when the external cream which mix | blended S-adenosylmethionine-containing liquid raw material was used.

In the production method of the raw material containing S-adenosylmethionine according to the first embodiment of the present invention, water and brown sugar are introduced into a culture tank, followed by a warm dissolution step of warm dissolving, followed by a warm dissolution step, followed by culture. A culture step in which yeast is introduced into a tank for cultivation, a separation step in which medium and yeast are separated by centrifugation after the cultivation step, and after the separation step, the obtained yeast is subjected to destruction of cells by lysis treatment. After the lysis treatment step and the lysis treatment step, an extraction step of extracting with water under acidic conditions, and the culture stop timing in the culture step is 15% turbidity from when the turbidity of the culture medium is switched from rising to falling. It was made until it deteriorated.

In the method for producing an S-adenosylmethionine-containing raw material according to the second embodiment of the present invention, water and brown sugar are introduced into a culture tank, followed by a warm dissolution step of warm dissolving and a warm dissolution step, followed by adding yeast to the culture tank. A cultivation step of injecting and culturing, a separation step of separating the medium and yeast by centrifugation after the cultivation step, and a lysis treatment step of dissolving cells by lysis treatment on the obtained yeast after the separation step, and a lysis treatment. After the process, it has an extraction process that performs extraction with water under acidic conditions.

According to these embodiments, an S-adenosylmethionine-containing raw material with a high content of S-adenosylmethionine can be obtained. In addition, since the obtained liquid S-adenosylmethionine-containing raw material is dissolved in water, it can also be blended into external preparations in cream, gel or liquid, cosmetics or health foods.

In the method for producing an S-adenosylmethionine-containing raw material according to a third embodiment of the present invention, water and brown sugar are added to a culture tank, and a warm dissolution step of warm dissolving and a warm dissolution step are followed by adding yeast to the culture tank. It has a culturing process to inject and cultivate, and a separation process to separate the medium and yeast by centrifugation after the cultivation process, and the timing of cultivation stop in the cultivation process is turbidity from when the turbidity of the culture medium is switched from rising to falling. Is until it decreases by 15%.

In the fourth embodiment of the present invention, in the method for producing the S-adenosylmethionine-containing raw material according to the third embodiment, the yeast is Cheongju yeast, and the temperature in the heating and dissolving step is 20 ° C or higher and 35 ° C or lower In the culture step, the temperature is 20 ° C or more and 35 ° C or less, the concentration of brown sugar is 6% or more and 10% or less, and the initial pH of the medium is 3 or more and 5 or less.

According to these embodiments, an S-adenosylmethionine-containing raw material with a high content of S-adenosylmethionine can be obtained.

In the fifth embodiment of the present invention, in the method for producing S-adenosylmethionine-containing raw materials according to the third or fourth embodiment, after the separation step, a drying step of freeze-drying the yeast to obtain a dry powder To have.

According to the present embodiment, a powdered S-adenosylmethionine-containing raw material with a high content of S-adenosylmethionine can be obtained.

The external preparation according to the sixth embodiment of the present invention contains an extract liquid raw material of yeast containing S-adenosylmethionine that has been subjected to lysis treatment.

The cosmetic product according to the seventh embodiment of the present invention contains an extract liquid raw material of S-adenosylmethionine-containing yeast subjected to lysis treatment.

The health food according to the eighth embodiment of the present invention contains an extract liquid raw material of yeasts containing S-adenosylmethionine lysed.

According to these embodiments, a cream-like, gel-like or liquid external preparation, cosmetic, or health food containing S-adenosylmethionine-containing liquid raw material can be obtained.

[Example]

Hereinafter, a method of manufacturing a raw material containing S-adenosylmethionine according to an embodiment of the present invention, and an external preparation, cosmetic, and health food in which S-adenosylmethionine-containing liquid raw material is blended will be described.

First, the manufacturing method of the raw material containing S-adenosylmethionine according to this Example will be described.

In the method for producing a raw material containing S-adenosylmethionine according to the present embodiment, brown sugar, which is a nutrient source for water and yeast, is added to a culture tank that has been sterile washed, and a nitrogen source is appropriately added to warm it to a predetermined temperature. It has a heating dissolution step to dissolve, and a culture step to inject and culture (ferment) yeast into the culture tank after the heating dissolution step, and a separation step to separate the medium and yeast by centrifugation after the culture step.

The predetermined temperature in the heating and dissolving step is preferably 20 ° C or more and 35 ° C or less, and more preferably 30 ° C.

Moreover, it is preferable to add a placenta extract powder as a nitrogen source in a warm melting process.

In addition, it is preferable to add the brown sugar only at the start of the warm melting process.

Yeast to be added after the heating dissolution process includes Saccharomyces, Candida, Torulopsis, Zygosaccharomyces, and Skizosaccharomyces. ), Edible yeast selected from the genus Pichia, Hansenula, Kluyveromyces, and Debaryomyces can be used. Especially, it is preferable to use the genus Saccharomyces, and it is more preferable to use the sake yeast among the genus Saccharomyces.

In the culturing step, the yeast is cultured (fermented), and S-adenosylmethionine is produced in the yeast.

The temperature in the culture step is preferably 20 ° C or more and 35 ° C or less, which is a temperature suitable for yeast growth, and more preferably 30 ° C. At low temperatures, the rate of yeast growth slows down, and at high temperatures, yeast dies out.

In addition, the concentration of brown sugar in the culturing step is preferably 3% or more and 10% or less, and more preferably 6% or more and 10% or less in weight ratio with respect to water.

In addition, the initial pH (the pH at the start of culture) of the culture solution in the culture step is preferably 3 or more and 7 or less, more preferably 3 or more and 5 or less, and most preferably 4.

The timing of the culture stop in the culture step is determined based on, for example, a change in the turbidity of the culture medium, a change in the pH of the medium, and the content of S-adenosylmethionine in yeast cells. In order to obtain S-adenosylmethionine-containing yeast having a high content of S-adenosylmethionine in the cells, the timing of the culture stop, when the turbidity of the culture medium changes from rising to falling (turning point), when turbidity decreases by 15% It's good to do so. That is, after the turbidity of the culture solution reaches the peak and starts to decrease, the culture stop timing is set until the turbidity decreases by 15% from the peak value. Further, it is more preferable to set the culture stop timing from the turning point until the turbidity decreases by 5%. In addition, when the peak switching point can be predicted in advance, it may be set before the peak switching point as the culture stop timing.

In the separation step, the yeast culture is passed through a continuous centrifuge to separate the medium from the yeast. S-adenosylmethionine-containing yeast can be obtained by separating the medium from yeast cells.

1 is a view showing the test results of different culture conditions (black sugar concentration in the medium, pH adjustment, culture time) in the culture step. The test is, It was carried out by incubation at 30C at 1t.

For measurement, the turbidity of the yeast culture solution was measured at 600 nm absorbance using an ultraviolet visible spectrophotometer (UV mini 1240 (Kabushiki Chemical Co., Ltd.)), and the yeast cell mass (OD 600 value) was measured. did.

Quantification (HPLC quantification) of S-adenosylmethionine content in yeast cells uses a device (type GL Sciences Inc. GL-7400), and a column (Chemco Scientific Co., Ltd.) ., Ltd.) Chemcopak Nucleosil 100-10SA), the mobile phase is a flow rate of 1.0 mL / min, 20 min at 0.05 M (NH ₄ ) ₂ HPO ₄ (pH3.0), and 5 min continuously With 0.5M (NH ₄ ) ₂ HPO ₄ (pH3.0) was performed at a linear slope to change the composition, it was detected at 260 nm. The method of analysis is by the method of Goto et al. ("Quantification and Change of S-adenosylmethionine in Cheongju sake lees" Nihonjo Kyokai, 1992).

The brown sugar concentration (weight ratio to water) of the medium in each test example and the initial pH of the medium are as follows. In addition, lactic acid was used for pH adjustment, and Na hydroxide was used for raising.

Test Example 1 ... Brown sugar concentration: 3%, initial pH: 4 (Fig. 1 (a))

Test Example 2 ... Brown sugar concentration: 6%, initial pH: 4 (Fig. 1 (b))

Test Example 3 ... Brown sugar concentration: 10%, initial pH: 4 (Fig. 1 (c))

Test Example 4 ... Brown sugar concentration: 6%, initial pH: 3 (Fig. 1 (d))

Test Example 5 ... Brown sugar concentration: 6%, initial pH: 5 (Fig. 1 (e))

Test Example 6 ... Brown sugar concentration: 6%, initial pH: 6 (Fig. 1 (f))

Test Example 7 ... Brown sugar concentration: 6%, initial pH: 7 (Figure 1 (g))

In FIG. 1, "◆" is OD 600 value (left axis), "▲" is S-adenosylmethionine content (left axis) in yeast cells, and "■" is pH (right axis). In addition, the content of S-adenosylmethionine in yeast cells is set to the highest result of 100.

When the initial pH was set to be the same and the difference in concentration of brown sugar was compared and compared, as shown in Figs. 1 (a) to (c), in Test Example 2 with a brown sugar concentration of 6%, the OD 600 value was a test example. It is higher than 1, and the content of S-adenosylmethionine in yeast cells is higher than that in Test Examples 1 and 3, and it is the best overall result.

In addition, when comparing by considering the difference in initial pH with the same concentration of brown sugar, as shown in Figs. 1 (b) and (d) to (g), Test Example 2 with an initial pH of 4 was OD. The 600 value was higher than Test Examples 4 and 7, and the S-adenosylmethionine content in the yeast cells was higher than Test Examples 4 to 7, and it was the best overall result.

Here, a method for determining the timing of cultivation stop in the cultivation process will be described by taking Test Example 2 (6% concentration of brown sugar in the medium and initial pH 4) as the overall best result.

Under the culture conditions of Test Example 2, as shown in Fig. 1 (b), when yeast enters the growth phase, the pH of the medium decreases in inverse proportion to the OD 600 value, and the slope of the curve toward the normal phase is gentle. It can be seen that it becomes constant around pH 3 at sunset, and immediately after entering the normal phase, the S-adenosylmethionine content (accumulation amount) is low, and the most S-adenosylmethionine content is present just before the death phase.

Therefore, the culture stop timing is set from when the turbidity of the culture medium is switched from rising to falling until turbidity decreases by 15%, more preferably until turbidity decreases by 5%. That is, in Test Example 2, since the peak of the OD 600 value is about 70, after the OD 600 value reaches the peak, until the OD 600 value reaches about 60, which is 85% of the peak value of about 70, the OD 600 value is more preferably at the peak. After reaching, there is a timing to stop the culture until the OD 600 value reaches about 66, which is 95% of the peak value of about 70.

This culture stop timing is within an elapsed time of 73 hours or more and 77 hours or less from the start of the culture step. That is, when viewed from the incubation time, it is preferable to be 75 hours ± 2 hours. In this way, the culture stop timing (culture time) can be determined by confirming the OD 600 value in the culture medium, the pH change of the medium, and the S-adenosylmethionine content in the cells.

As described above, according to the method for producing the S-adenosylmethionine-containing raw material according to the present embodiment, S-adenosylmethionine-containing yeast having a large amount of S-adenosylmethionine in yeast cells can be obtained.

Next, the production method of the powdered S-adenosylmethionine-containing raw material (hereinafter referred to as "S-adenosylmethionine-containing yeast powder raw material") according to this embodiment will be described.

The method for producing the raw material for yeast powder containing S-adenosylmethionine according to the present embodiment is a non-heat sterilization of the obtained S-adenosylmethionine containing yeast after the separation step in the method for producing raw material containing S-adenosylmethionine It has a sterilization process to be performed and a drying process to obtain dry powder by freeze-drying the yeast containing S-adenosylmethionine after the sterilization process.

Through these steps, S-adenosylmethionine-containing yeast is used as a dry powder, for example, S-adenosylmethionine-containing yeast powder containing 10% or more of S-adenosylmethionine can be obtained. The obtained S-adenosylmethionine-containing yeast powder can be used as a raw material for health foods and the like.

Next, a method for producing a liquid S-adenosylmethionine-containing raw material (hereinafter referred to as "S-adenosylmethionine-containing liquid raw material") according to this embodiment will be described.

The method for producing an S-adenosylmethionine-containing liquid raw material according to the present embodiment is to non-heat sterilize the obtained S-adenosylmethionine-containing yeast after the separation step in the aforementioned method for producing an S-adenosylmethionine-containing raw material. It has a sterilization step and a sterilization treatment step in which bacterial cells are destroyed by lysis treatment for yeasts containing S-adenosylmethionine after the sterilization step, and an extraction step of extracting with water under acidic conditions after the lysis treatment step.

For S-adenosylmethionine-containing yeasts, after lysis treatment, extracting with water under acidic conditions to stabilize S-adenosylmethionine and filtering the lysate extract, a liquid raw material containing S-adenosylmethionine can be obtained. . The obtained S-adenosylmethionine-containing liquid raw material can be used as a raw material for external preparations, cosmetics or health foods.

Since the conventional S-adenosylmethionine-containing raw material is powdery, it is not soluble in water or oil, and it has been difficult to blend into products such as creams, gels, or liquid external preparations, cosmetics or health foods. If S-adenosylmethionine-containing yeast powder raw materials are tried to be blended into a creamy product, there is no choice but to mix them into the cream, and since it cannot stably and uniformly blend into the cream, the blending of only 0.5% was the limit. Compared to this, the liquid raw material containing S-adenosylmethionine according to the present embodiment is liquid unlike the raw material for yeast powder containing S-adenosylmethionine, and is soluble in water. It can be done and can be blended up to 70%. Therefore, by using S-adenosylmethionine-containing liquid raw materials, products such as cream, gel, or liquid external preparations, cosmetics, or health foods containing a lot of S-adenosylmethionine can be realized.

Moreover, it is preferable that the liquid raw material containing S-adenosylmethionine contains 1 ppm or more of S-adenosylmethionine and pH is 3 or more and 5 or less.

2 and 3 are external creams containing S-adenosylmethionine-containing liquid raw material in the knee of a subject with relatively mild deformed knee joint, to confirm the effect of S-adenosylmethionine on external use. It is a figure which shows the result which apply | coated and confirmed the progress.

As an evaluation method, an external cream containing S-adenosylmethionine-containing liquid raw material was applied to the subject's knee three times a day, and every two weeks, VAS (Visual Analog Scale: visual evaluation scale ... The level of pain in the knee was evaluated in the method of digitizing by marking on a horizontal straight line, and functional evaluation was performed. The evaluation period was 12 weeks (3 months), and the number of subjects was 11 from the start to 8 weeks later, but after that, the number of subjects decreased by 1, and finally the total number of subjects was 10. In addition, evaluation was referred to an orthopedic surgeon.

2 is the average score of VAS, and shows the transition of pain in the knee joint when an external cream containing S-adenosylmethionine-containing liquid raw material is used. As shown in Fig. 2, an external cream containing S-adenosylmethionine-containing liquid raw material was used for 12 weeks, but pain in the knee joint was alleviated.

It is a figure which shows the result of the functional evaluation when the external cream which mix | blended S-adenosylmethionine-containing liquid raw material was used. Fig. 3 (a) is a stair climb, Fig. 3 (b) is a stair climb, Fig. 3 (c) is walking on the flat, Fig. 3 (d) is kneeling, and Fig. 3 (e) is standing up.

As shown in Figs. 3 (a) and 3 (b), as for the up and down of the stairs, subjects who feel "slightly difficult" are decreasing after 12 weeks from the start. In particular, about 80% of subjects who felt "slightly difficult" at the start of the stairs, but about 60% of them felt "no problem" after 12 weeks. Thus, improvement of the functionality was recognized for the up and down of the stairs.

As shown in Fig. 3 (c), about 40% or more of the subjects who felt "slightly difficult" at the start of walking on the flatland, but after 12 weeks, the power all felt "no problem". As described above, improvement in functionality was also recognized for walking on flat land.

As for kneeling, as shown in Fig. 3 (d), at the start, about 80% of the subjects felt "extremely difficult", but after 12 weeks, the power all felt "no problem". Thus, improvement in functionality was also recognized for kneeling.

As shown in Fig. 3 (e), when standing up, about 90% of the subjects felt "extremely difficult" or "slightly difficult" at the start, but after 12 weeks, there was no problem. Subject occupied approximately 80%. Thus, improvement of the functionality was also recognized for standing up.

Therefore, by applying the external cream containing S-adenosylmethionine-containing liquid raw material, relief of pain in the knee joint and improvement of daily operation were recognized.

The raw material containing S-adenosylmethionine according to the production method of the present invention can be used as a raw material for external preparations, cosmetics or health foods. In addition, the liquid raw material containing S-adenosylmethionine can also be blended into products in cream, gel, or liquid form.

Claims (8)

A heating dissolving process in which water and brown sugar are added to a culture tank and heated and dissolved,
After the warming and dissolving step, the culture step of culturing by introducing yeast into the culture tank,
After the culturing process, the separation process to separate the medium (培 地) and the yeast by centrifugation,
After the separation step, a lysis treatment step for destroying the cells by lysis treatment on the obtained yeast,
After the lysis treatment step, it has an extraction step of performing extraction with water under acidic conditions,
The cultivation stop timing in the cultivation step is set from the time when the culture medium pH becomes constant around 3, and the turbidity of the culture medium is changed from rising to falling until the turbidity decreases by 15%.
After the extraction process, the bacterial extract is filtered, and the S-adenosylmethionine-containing liquid raw material is blended, characterized in that the liquid raw material containing S-adenosylmethionine-containing liquid is prepared. A heating dissolving process in which water and brown sugar are added to a culture tank and heated and dissolved,
After the warming and dissolving step, the culture step of culturing by introducing yeast into the culture tank,
After the culturing process, the separation process to separate the medium (培 地) and the yeast by centrifugation,
After the separation step, a lysis treatment step for destroying the cells by lysis treatment on the obtained yeast,
After the lysis treatment step, it has an extraction step of performing extraction with water under acidic conditions,
The cultivation stop timing in the cultivation step is set from the time when the culture medium pH becomes constant around 3, and the turbidity of the culture medium is changed from rising to falling until the turbidity decreases by 15%.
After the extraction process, the bacterial extract is filtered, and the S-adenosylmethionine-containing liquid raw material is blended. A heating dissolving process in which water and brown sugar are added to a culture tank and heated and dissolved,
After the warming and dissolving step, the culture step of culturing by introducing yeast into the culture tank,
After the culturing process, the separation process to separate the medium (培 地) and the yeast by centrifugation,
After the separation step, a lysis treatment step for destroying the cells by lysis treatment on the obtained yeast,
After the lysis treatment step, it has an extraction step of performing extraction with water under acidic conditions,
The cultivation stop timing in the cultivation step is set from the time when the culture medium pH becomes constant around 3, and the turbidity of the culture medium is changed from rising to falling until the turbidity decreases by 15%.
A method for producing a creamy, gel, or liquid health food comprising a liquid raw material containing S-adenosylmethionine, wherein the lytic extract is filtered after the extraction step, and the obtained liquid raw material containing S-adenosylmethionine is blended. According to claim 1,
The yeast is Cheongju yeast,
The temperature in the heating dissolution step is 20 ° C or more and 35 ° C or less,
In the culture step, the temperature is 20 ° C or more and 35 ° C or less, the concentration of the brown sugar is 6% or more and 10% or less by weight with respect to the water, the initial pH of the medium is 4, and the brown sugar starts the warm dissolution process A method for preparing a cream, gel or liquid external preparation containing S-adenosylmethionine-containing liquid raw material, characterized in that it is added only to the si. According to claim 2,
The yeast is Cheongju yeast,
The temperature in the heating dissolution step is 20 ° C or more and 35 ° C or less,
In the culture step, the temperature is 20 ° C or more and 35 ° C or less, the concentration of the brown sugar is 6% or more and 10% or less by weight with respect to the water, the initial pH of the medium is 4, and the brown sugar starts the warm dissolution process A method for producing a creamy, gel or liquid cosmetic product comprising a liquid raw material containing S-adenosylmethionine, characterized in that it is added only to the si. The method of claim 3,
The yeast is Cheongju yeast,
The temperature in the heating dissolution step is 20 ° C or more and 35 ° C or less,
In the culture step, the temperature is 20 ° C or more and 35 ° C or less, the concentration of the brown sugar is 6% or more and 10% or less by weight with respect to the water, the initial pH of the medium is 4, and the brown sugar starts the warm dissolution process A method for producing a health food in a creamy, gel or liquid form in which a liquid raw material containing S-adenosylmethionine is blended, characterized in that it is added only to the sieve. delete delete

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