KR102087868B1 - Cosmetic composition containing the extracts of natural substances, Stellaria media, Helianthus annuus and Vaccinium vitis-idaea - Google Patents

Abstract

The present invention relates to a cosmetic composition containing, as an active ingredient, a composite extract prepared by subcritical extraction after the ultra-high pressure pretreatment of a mixture of Stellaria media, Helianthus annuus and Vaccinium vitis-idaea. The prepared composite extract exhibits excellent skin cell protection and anti-aging efficacy under condition involving UV and blue light irradiation that could cause skin cell damage and aging, and effectively inhibits the penetration of blue light. Due to the effects, the composite extract of the present invention can be useful as a cosmetic ingredient for preventing skin aging or blocking blue light due to ultraviolet rays and blue light.

Description

Cosmetic composition containing chickweed, sunflower and bilberry extract as an active ingredient {Cosmetic composition containing the extracts of natural substances, Stellaria media, Helianthus annuus and Vaccinium vitis-idaea}

The present invention relates to a cosmetic composition containing chickweed, sunflower and bilberry complex extract, specifically, Stellaria media , sunflower ( Helianthus annuus ) and bilberry ( Viccinium vitis-idaea ) mixture by ultracritical extraction after ultra high pressure pretreatment It contains a chickweed, sunflower and bilberry complex extract prepared as an active ingredient, and protects the skin from blue light and ultraviolet rays which have a harmful effect on the skin, and relates to a cosmetic composition showing excellent anti-aging activity.

Skin aging can be classified into intrinsic aging according to time-dependent natural aging and extrinsic aging due to external factors. Exogenous aging factors include various factors such as wind, temperature, and pollution, but the most important factor is ultraviolet rays. Endogenous aging is characterized by fine wrinkles, pale skin, dry skin, and slight loss of elasticity, while exogenous aging is more rapid and significant signs of aging such as thick and deep wrinkles, decreased elasticity, dry skin, and pigmentation. 2013; Jeon So-hyun et al., 2014).

Continuous UV exposure induces an increase in reactive oxygen species (ROS) in the skin, causing pigmentation due to irregular melanogenesis of melanocytes and irregularly changing the arrangement of keratinocytes to reduce skin moisturization. It also increases the production of matrix metalloproteinase-1 (MMP-1), which promotes the breakdown of collagen in fibroblasts, and reduces the production of procollagen type 1, a precursor of collagen, which induces wrinkles and reduces elasticity, resulting in accelerated skin aging. (Kim Ok-kyung et al., 2013). Recent skin research has shown that not only ultraviolet light but also blue light can be an important cause of skin aging. Blue light has been shown to reduce skin cell survival, increase ROS production, activate skin aging mechanisms such as increased MMP-1 production, reduced collagen production, and increased DNA damage (Avola R. et al. , 2019). Therefore, in developing products for inhibiting skin aging, it is most important to find a material that can control skin factors that are changed by ultraviolet light and blue light.

Natural materials for inhibiting skin aging caused by ultraviolet rays have been studied in the past, but the material for inhibiting skin aging due to blue light has been studied only relatively recently. A study paper recently published that olive, resveratrol, etc. inhibits skin cell damage caused by blue light (Avola R. et al., 2019; Mamalis A. et al., 2016). Dozens of blue light and skin related patents have been searched so far, but most of them are mainly related to LED beauty devices, and a few have been found that can block and protect blue light from the skin. Related patents include a blue light protective cosmetic composition (Korean Patent Publication No. 10-2019-0006910), a blue light blocking cosmetic composition (Korean Patent Registration No. 10-1851623) containing spirulina extract as an active ingredient. Other harmful visible light blocking cosmetic composition (Korean Patent Publication No. 10-2017-0101799), blue light blocking cosmetic composition containing jade powder (Korean Patent Publication No. 10-2017-0003391), ultraviolet rays and blue light Titanium particles having excellent absorption ability and a manufacturing method thereof (Korea Patent No. 10-1905418) and the like have been disclosed.

Stellaria media is a vascular plant belonging to the Sukjukokseok family, and is a biennial plant that grows commonly in sunny, low-lying areas such as roadsides, vacant lands, and field dunes. It is similar to Stellaria aquatica , but it is smaller in size than the stellar flower and has three pistils and is distinct from five stigmas. It eats young shoots and flies all over Korea.

Sunflower ( Helianthus annuus ) is a flowering plant belonging to the chrysanthemum Asteraceae. It is a natural plant native to North America, and is an annual herb that is planted in fields or flower beds for ornamental and food use. A similar naturalized plant native to North America, Helianthus salicifolius does not have a leaf directly under the head flower, and is 5 ~ 9cm in diameter and small, and the tip of the lanceolate bundle is sharp in the shape of a tail. Flowers are for ornamental purposes. Fruits are used for edible or cooking oil, and they are planted throughout Korea.

The bilberry ( Vaccinium vitis-idaea ) is a flowering plant belonging to the rhododendron Azalea, an evergreen small evergreen tree that rarely grows in high mountain forests. Flowers bloom in May-June, white or pink, and hang 2 ~ 3 each on a 1 ~ 2cm long inflorescence from the axilla at the tip of last year's branches. Fruits are round berries and ripen in red from August to September. This species is a small bush compared to Vaccinium bracteatum , with two or three flowers, and ripened red. Planted for ornamental purposes, leaves are medicinal, fruits are edible. It grows wild on the Korean peninsula.

The present inventors conducted a study to discover a natural material composite extract that can exhibit the skin protection effect from ultraviolet light and blue light, and finally selected three materials of chickweed, sunflower and bilberry through primary screening. When the mixture is mixed with these and then subcritically extracted and prepared as a complex extract, the effect of increasing the skin aging-inducing factor and the decrease of skin collagen precursor due to blue light or ultraviolet ray is greatly enhanced, and also shows the blue light permeation inhibitory activity. It was confirmed that the present invention was completed.

It is an object of the present invention to provide a cosmetic composition containing chickweed, sunflower and bilberry composite extract prepared by subcritical extraction after ultra high pressure pretreatment as an active ingredient.

In another aspect, the present invention is to provide a method for producing a chickweed, sunflower and bilberry complex extract excellent in the activity of inhibiting skin aging due to ultraviolet light and blue light.

According to the present invention in order to achieve the above object is provided a cosmetic composition containing 0.01 to 15% by weight relative to the total weight of the cosmetic composition as an active ingredient is prepared by extracting under subcritical conditions after ultra-high pressure pretreatment. .

The composite extract is produced by mixing and extracting dried chickweed, sunflower and bilberry in a weight ratio of 1 to 5: 1 to 5: 1 to 5, respectively.

The cosmetic composition is characterized in that for inhibiting skin aging due to ultraviolet light and blue light. In addition, the cosmetic composition is characterized in that the blue light blocking.

According to the present invention to achieve the above another object, (A) mixing the chickweed, sunflower and bilberry at a weight ratio of 1 to 5: 1 to 5: 1 to 5, respectively, to prepare a mixture; (B) a pretreatment step of applying the extraction solvent to the mixture itself or the mixture, and then pressurized to ultra high pressure; And (C) is added to the extraction solvent is provided a method for producing a star flower, sunflower and bilberry complex extract comprising the step of extracting for 10 to 30 minutes at 120 ~ 200 ℃, the pressure of 0.1 ~ 15MPa subcritical conditions.

The extraction solvent in the step (B) or (C) is water, anhydrous alcohol of 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethylene glycol, propylene glycol, butylene glycol, caprylic / capric triglyceride, At least one selected from the group consisting of dimethicone, mineral oil, cyclomethicone, octyldodecanol, cetylethylhexanoate, triethylhexanoin, isopropyl myristate and vegetable oil is used.

The pretreatment of step (B) is more preferably made by applying the extraction solvent to the mixture or the mixture in a plastic pouch and sealed and pressurized for 60 to 180 minutes with water at 40 ~ 50 ℃, 50 ~ 100MPa conditions. .

Starflower, sunflower and bilberry complex extract according to the present invention is made of a natural extract is safe, and by its synergism, inhibits skin aging due to ultraviolet rays and blue light, anti-aging cosmetics because it is very excellent in inhibiting the transmission of blue light It can be usefully used.

1 is a photograph showing the blue light blocking activity test results of chickweed, sunflower and bilberry complex extract according to an embodiment of the present invention.

Hereinafter, the content of the present invention will be described in detail in order to help understanding of the present invention.

According to the present invention there is provided a cosmetic composition containing 0.01 to 15% by weight relative to the total weight of the cosmetic composition as the active ingredient chickweed, sunflower and bilberry complex extract prepared by subcritical extraction after ultra-high pressure pretreatment.

The chickweed, sunflower and bilberry complex extract is prepared by a method comprising the following steps.

According to an embodiment of the present invention, (A) mixing the chickweed, sunflower and bilberry in a weight ratio of 1 to 5: 1 to 5: 1 to 5, respectively, to prepare a mixture; (B) a pretreatment step of applying the extraction solvent to the mixture itself or the mixture, and then pressurized to ultra high pressure; And (C) adding a solvent to extract for 10 to 30 minutes at 120 to 200 ° C., which is a subcritical condition, and a pressure of 0.1 to 15 MPa.

First, the dried chickweed, sunflower and bilberry are mixed in a weight ratio of 1-5: 1-5: 1-5 to prepare a mixture. At this time, chickweed outpost that can be used as a component of the cosmetic is used, sunflower outpost, flowers and / or seeds are used, bilberry fruit, leaves and / or seeds are used.

Subsequently, the mixture itself or the mixture is water, anhydrous alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethylene glycol, propylene glycol, butylene glycol, caprylic / capric triglycerides, dimethicone, mineral oil, Cyclomethicone, octyldodecanol, cetylethylhexanoate, triethylhexanoin, isopropyl myristate and at least one extraction solvent selected from the group consisting of vegetable oils were added and then pressurized to an ultra-high pressure of 50-100 MPa. Pretreatment The vegetable oil refers to an oil extracted from a plant seed or pulp by compression or the like, and may be used as olive oil, sunflower seed oil, jojoba oil, palm oil, avocado oil, and the like.

More preferably, the pretreatment is performed by adding the extraction solvent to the mixture or the mixture in a plastic pouch and sealing it, and pressurizing for 60 to 180 minutes using water at 40 to 50 ° C. and 50 to 100 MPa conditions as a pressure medium. .

 Thereafter, an extraction solvent is added to the mixture, which is pretreated, and extracted under subcritical conditions, filtered, and concentrated to prepare a chickweed, sunflower and bilberry complex extract. In this case, when the mixture is pressurized with the extraction solvent in the pretreatment process, the extraction process may be performed under subcritical conditions.

At this time, when the chickweed, sunflower and bilberry were mixed and extracted at a weight ratio of 1 to 3: 3 to 5: 1 to 3, respectively, they showed more excellent activity, and each was mixed to a weight ratio of 3: 5: 1. It showed the best activity when.

The composite extract of chickweed, sunflower and bilberry prepared in this way is superior to ultraviolet and blue light as compared to the composite extract of chickweed, sunflower or bilberry, respectively, or extracted by conventional methods or extracted only in subcritical conditions without pretreatment. It showed skin cell protection and anti-aging effect, and confirmed the synergistic effect between the active ingredients according to the mixing.

The chickweed, sunflower, and bilberry complex extracts of the present invention not only irradiated with UV and blue light, but also irradiated with ultraviolet and blue light without affecting the survival rate of skin-derived cell lines even in the irradiated state (Test Examples 1 and 2). Under the conditions, the generation of skin aging-inducing factor ROS (Test Example 3) was effectively suppressed. In addition, the mRNA expression of MMP-1 (Test Example 4) was reduced under UV and blue light irradiation conditions, and procollagen type 1 production was greatly increased (Test Example 5). In addition, the chickweed, sunflower and bilberry composite extract of the present invention showed an effect of greatly reducing the transmission of blue light (Test Example 6). When the complex extract was applied to serum and cream formulations, it showed formulation safety at room temperature, refrigeration, constant temperature, and circulation conditions (Test Example 7).

Therefore, the chickweed, sunflower and bilberry complex extract can be usefully used as a cosmetic for inhibiting skin aging due to ultraviolet rays and blue light and cosmetics for blocking blue light.

The chickweed, sunflower and bilberry complex extract may be contained 0.01 to 15% by weight relative to the total weight of the cosmetic composition as an active ingredient.

 The cosmetic composition of the present invention may be prepared in any of the formulations commonly prepared, such as lotion, cream, essence, cleansing foam, cleansing water, feminine cleanser, pack, body lotion, body oil, body gel, shampoo, Rinse, hair conditioner, hair gel, foundation, lipstick, mascara, makeup base and the like.

EXAMPLE

Hereinafter, examples will be described in detail to help understand the present invention. However, the following examples are merely to illustrate the content of the present invention, the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Preparation Examples 1-3: Preparation of chickweed, sunflower or bilberry extract

5 g of purified water was added to 100 g of dried chickweed outpost, sunflower flower and bilberry fruit as extraction solvent, and the extract was heated at 95 ° C. in an extractor equipped with a cooling condenser (Cosmos-660, Gyeongseo Machinery) for 2 hours.

 After extraction by the above method, the mixture was left at room temperature for 3 days, and the precipitate was filtered through a 300 mesh filter paper, and the precipitate was filtered twice with an Adventech No. 5 filter paper and a Whatman GFC 150 mm filter paper to prepare an extract.

Preparation Example 4-6 Preparation of Chickweed, Sunflower or Bilberry Subcritical Extract

To 100 g of dried chickweed outpost, sunflower flower and bilberry fruit, 5 times the weight of purified water was added as an extraction solvent, and extracted for 15 minutes at 200 ° C. under pressure of 15 MPa.

 After extraction by the above method, the mixture was left at room temperature for 3 days, and the precipitate was filtered through a 300 mesh filter paper, and the precipitate was filtered twice with an Adventech No. 5 filter paper and a Whatman GFC 150 mm filter paper to prepare an extract.

Preparation Examples 7-11: Preparation of Chickweed, Sunflower and Bilberry Subcritical Complex Extracts

Dried chickweed outpost, sunflower flower and bilberry fruit was mixed in the weight ratio of Table 1 to prepare a mixture of 100g, 5 times the weight of purified water and extracted for 15 minutes at 200 ℃, pressure 15 MPa of subcritical conditions.

 After extraction by the above method, the mixture was left at room temperature for 3 days, and the precipitate was filtered through a 300 mesh filter paper, and the precipitate was filtered twice with an Adventech No. 5 filter paper and a Whatman GFC 150 mm filter paper to prepare an extract.

Examples 1-5 5: Preparation of Chickweed, Sunflower and Bilberry Ultra High Pressure Pretreatment Subcritical Complex Extracts

The dried chickweed outpost, sunflower flower and bilberry fruit were mixed in a weight ratio of Table 1 to prepare a mixture of 100g, and then put in a plastic pouch and sealed and pressurized at an ultra-high pressure for 120 minutes with water at 50 ℃, 100MPa (TFS -10L, Toyo Koatsu Co. LTD. Then, after removing the vinyl pouch, 5 times the weight of purified water was added to the pretreated chickweed, sunflower and bilberry mixture and extracted for 15 minutes at 200 ° C. under pressure of 15 MPa.

After extraction by the above method, the mixture was left at room temperature for 3 days, and the precipitate was filtered through a 300 mesh filter paper, and the precipitate was filtered twice with an Adventech No. 5 filter paper and a Whatman GFC 150 mm filter paper to prepare an extract.

(Weight ratio) chickweed sunflower Bilberry Preparation Example 7 Example 1 One One One Preparation Example 8 Example 2 5 3 One Preparation Example 9 Example 3 3 One 5 Preparation Example 10 Example 4 One 5 3 Preparation Example 11 Example 5 3 5 One

Test Example 1: Confirmation of Cytotoxicity

To determine the cytotoxicity, keratinocyte lines HaCaT and fibroblast NHDF (normal human dermal fibroblast) were cultured in DMEM medium with 10% FBS and 5 × 10 ⁵ and 3 × 10 ⁴ cells in 96 well plates, respectively. Inoculation at concentration was incubated for 24 hours in a 37 ℃ 5% CO ₂ incubator. After culturing, the medium was removed, and the extract prepared in Examples and Examples above was diluted with dimethyl sulfoxide so as to have a sample concentration of 0.1 or 1.0%, followed by incubation for 24 hours, followed by MTT (3- [4). , 5-dimethylthiazol-2yl] -2,5-diphenyltetrazoliumboromide, Sigma) solution was added to each well 100ml (3 mg / ml) and further incubated for 4 hours. Then, the supernatant was removed, 150 μl of dimethyl sulfoxide was added, and then shaken for 30 minutes to dissolve the generated formazan, and the absorbance was measured at 540 nm using a multimicroplate reader (Molecular device Spectra max190). Cell viability was calculated according to the following formula and the results are shown in Table 2 below.

Cell survival rate (%) = absorbance of the sample addition group / absorbance of the control group × 100

division Cell survival rate (%) HaCAT NHDF 0.1% 1.0% 0.1% 1.0% Control 100 Preparation Example 1 101 100 100 100 Preparation Example 2 101 102 100 101 Preparation Example 3 100 100 100 100 Preparation Example 4 101 100 99 98 Preparation Example 5 100 100 101 100 Preparation Example 6 100 99 101 101 Preparation Example 7 101 98 99 100 Preparation Example 8 100 101 100 99 Preparation Example 9 100 103 100 100 Preparation Example 10 101 98 99 98 Preparation Example 11 101 101 100 99 Example 1 100 100 100 100 Example 2 99 101 100 99 Example 3 101 100 99 96 Example 4 98 101 100 100 Example 5 100 101 100 101

As shown in the results of Table 2, it was confirmed that it does not affect the cytotoxicity of human skin-derived cells as it shows a cell viability of 95% or more in all samples to which the chickweed, sunflower or bilberry extract and their mixed extracts are applied. It was confirmed that there is no problem with safety.

Test Example 2: Confirmation of cytotoxicity under UV and blue light irradiation conditions

To determine cytotoxicity under UV and blue light irradiation conditions, HaCaT and NHDF cells were inoculated into 24 well plates at cell concentrations of 6 × 10 ⁶ and 4 × 10 ⁵ , respectively, for 24 hours in a 37 ° C. 5% CO ₂ incubator. Incubated for 2 hours. After incubation, the medium was removed, washed once with PBS (phosphate buffered saline), and 1 ml of PBS was added to each well. In order to check the cytotoxicity caused by UV light, the plate was placed in a UV irradiator (BLX-312, Vilber Lourmal, France) and irradiated with UV light at a light quantity of 75 mJ / cm ² using a UV lamp. Cytotoxicity by blue light was confirmed after irradiation for 30 minutes using an LED-Blue light lamp (HBL0204, Jeno, Korea) of 440 ~ 450 nm wavelength. After irradiating each ray, the PBS contained in the plate was removed and the extract prepared in Preparation Example and Example was treated to have a sample concentration of 1.0%. After incubation for 24 hours, the MTT experiment was performed in the same manner as that described in Test Example 1 to confirm the effect of the extract sample on cytotoxicity. Cell viability was calculated using the absorbance of the ultraviolet and blue light irradiation group as a control group and the results are shown in Table 3 below.

division Cell survival rate (%) UV-rays Blue light HaCaT NHDF HaCaT NHDF Light irradiation X 142 134 128 117 Control 100 Preparation Example 1 101 100 99 97 Preparation Example 2 102 105 102 99 Preparation Example 3 105 104 100 100 Preparation Example 4 104 101 101 101 Preparation Example 5 103 99 103 100 Preparation Example 6 102 104 100 99 Preparation Example 7 101 106 104 101 Preparation Example 8 105 100 102 98 Preparation Example 9 99 98 101 102 Preparation Example 10 101 97 100 100 Preparation Example 11 102 105 106 103 Example 1 101 103 98 99 Example 2 105 104 99 101 Example 3 98 105 106 102 Example 4 102 100 101 100 Example 5 101 105 105 100

As shown in the results of Table 3, all of the samples to which the chickweed, sunflower or bilberry extract and mixed extracts thereof, etc. exhibited cell viability of 95% or more compared to the control, and thus did not affect the cytotoxicity of human skin-derived cells. It was confirmed.

Test Example 3: Confirmation of ROS generation inhibitory effect under UV and blue light irradiation conditions

In order to confirm the cytotoxicity under UV and blue light irradiation conditions, HaCaT and NHDF cells were inoculated in 24 well plates and incubated in 37 ° C. 5% CO ₂ incubator for 24 hours. After incubation, the medium was removed, washed once with PBS (phosphate buffered saline), 1 ml of PBS was added to each well, and the UV and blue lights were irradiated in the same manner as described in Test Example 2. Thereafter, the extracts prepared in Preparation Examples and Examples were treated to have a sample concentration of 1.0% and then incubated for 24 hours. Remove the culture medium, add 40 μM of DCF-DA (2'-7'-dichlorofluoresceine diacetate) dissolved in DMEM medium containing no phenol red, and after 30 minutes, pick up the supernatant of each well and attach a fluorometer with an ELISA reader. Fluorescence was measured at excitation 485 nm and emission 530 nm. The ROS production rate was calculated using the fluorescence of the ultraviolet and blue light irradiation groups as a control, and the results are shown in Table 4 below.

division ROS production rate (%) UV-rays Blue light HaCaT NHDF HaCaT NHDF Light irradiation X 8 11 4 9 Control 100 Preparation Example 1 67 72 69 58 Preparation Example 2 66 71 65 59 Preparation Example 3 64 73 68 57 Preparation Example 4 58 64 62 49 Preparation Example 5 56 66 61 50 Preparation Example 6 55 67 60 47 Preparation Example 7 48 60 51 41 Preparation Example 8 46 60 52 40 Preparation Example 9 45 62 52 41 Preparation Example 10 42 54 48 34 Preparation Example 11 40 53 46 32 Example 1 34 45 40 22 Example 2 33 46 39 23 Example 3 34 44 41 21 Example 4 29 40 34 14 Example 5 22 37 31 10

As shown in the results of Table 4, the chickweed, sunflower and bilberry complex extract of the present invention prepared by subcritical extraction after ultra-high pressure pretreatment compared to the respective extracts of the preparation, in the skin-derived cell line UV and blue light Inhibition of the production of ROS is shown to be excellent, it was confirmed that the effect of inhibiting skin aging due to ultraviolet light and blue light. In particular, the high inhibitory activity was shown in Examples 4 and 5, the most excellent activity in Example 5.

Test Example 4 Confirmation of MMP-1 Expression Inhibitory Effect Under UV and Blue Light Irradiation Conditions

In order to confirm the effect of inhibiting MMP-1 expression under UV and blue light irradiation conditions, NHDF cells were inoculated into 6 well plates and incubated in 37 ° C. 5% CO ₂ incubator for 24 hours. After incubation, the medium was removed, washed once with PBS (phosphate buffered saline), 1 ml of PBS was added to each well, and the UV and blue light were irradiated in the same manner as described in Test Example 2. Thereafter, the extracts prepared in Preparation Examples and Examples were treated to a sample concentration of 0.1 or 1.0%, and then incubated for 24 hours. Thereafter, NHDF cells were recovered using Trizol reagent (invitrogen, USA), mRNA was extracted, and cDNA was synthesized through a series of processes. The amount of gene expression was confirmed by electrophoresis by amplifying the MMP-1 gene region from the synthesized cDNA, and gene amplification was performed using a thermal cycler (GenePro, Hangzhou bioer tech. 10 pmol primer (F: GGT GAT GAA GCA GCC CAG, R: CAG TAG AAT GGG AGA GTC) and taq polymerase Minutes / 72 degrees 1 min 28 cycle amplification, was carried out under the conditions of reacting for 5 minutes at 72 degrees. MMP-1 expression was calculated using the expression level of the ultraviolet and blue light irradiation group as a control group and the results are shown in Table 5 below.

division MMP-1 expression level (%) UV-rays Blue light 0.1% 1.0% 0.1% 1.0% Light irradiation X 46 35 Control 100 Preparation Example 1 74 67 77 68 Preparation Example 2 75 68 76 67 Preparation Example 3 76 66 75 65 Preparation Example 4 72 62 74 65 Preparation Example 5 70 60 72 63 Preparation Example 6 71 58 73 63 Preparation Example 7 67 52 70 58 Preparation Example 8 68 51 69 57 Preparation Example 9 66 53 68 57 Preparation Example 10 61 48 64 52 Preparation Example 11 60 46 61 49 Example 1 56 40 60 46 Example 2 57 41 61 48 Example 3 56 39 59 45 Example 4 52 34 56 40 Example 5 49 32 52 36

As shown in the results of Table 5, the chickweed, sunflower and bilberry composite extract of the embodiment of the present invention prepared by subcritical extraction after ultra high pressure pretreatment, the respective extracts of Preparation Examples 1 to 6, the ultra high pressure pretreatment of the present invention Compared with the composite extracts of Examples 7 to 11, it was confirmed that the human fibroblasts effectively suppressed the increase of MMP-1 expression by ultraviolet or blue light. In particular, Example 5 showed the best activity.

Test Example 5 Confirmation of the increase in procollagen type 1 production under UV and blue light irradiation conditions

In order to confirm the inhibitory effect of procollagen type 1 production under UV and blue light irradiation conditions, NHDF cells were inoculated in 24 well plates and incubated in 37 ° C. 5% CO ₂ incubator for 24 hours. After incubation, the medium was removed, washed once with PBS (phosphate buffered saline), 1 ml of PBS was added to each well, and UV and blue light were irradiated in the same manner as described in Test Example 2. Thereafter, the extracts prepared in Preparation Examples and Examples were treated to a sample concentration of 0.1 or 1.0%, and then incubated for 24 hours. Thereafter, the cultured cells were collected and the amount of procollagen type 1 was measured using a Procollagen type I c-peptide EIA kit (TAKARA, MK101). On the other hand, cells attached to the bottom was washed with PBS and lysed with 1N NaOH to measure the total protein amount to calculate the amount of procollagen per constant protein. The results are shown in Table 6 below.

division Procollagen type 1 production / total protein UV-rays Blue light 0.1% 1.0% 0.1% 1.0% Light irradiation X 5.3 Control 2.8 3.2 Preparation Example 1 2.8 2.9 3.2 3.3 Preparation Example 2 2.9 2.9 3.2 3.2 Preparation Example 3 2.9 3.0 3.3 3.4 Preparation Example 4 3.1 3.2 3.5 3.6 Preparation Example 5 3.2 3.3 3.4 3.6 Preparation Example 6 3.2 3.3 3.5 3.7 Preparation Example 7 3.8 3.9 3.7 4.0 Preparation Example 8 3.7 3.9 3.8 3.9 Preparation Example 9 3.8 4.0 3.7 4.0 Preparation Example 10 4.1 4.2 4.0 4.3 Preparation Example 11 4.2 4.3 4.1 4.3 Example 1 4.0 4.5 4.3 4.5 Example 2 4.2 4.4 4.4 4.6 Example 3 4.3 4.5 4.3 4.8 Example 4 4.8 4.9 4.7 5.3 Example 5 4.9 5.2 4.8 5.5

As shown in the results of Table 6, the chickweed, sunflower and bilberry composite extract of the present invention is compared to the respective extracts of the preparation, the composite extract of the pre-treatment example, in the ultraviolet and blue light in human fibroblasts It was confirmed that it effectively inhibits the reduction of procollagen type 1 production.

Experimental Example 6: Checking Blue Light Transmission

The blue light blocking activity of the chickweed, sunflower and bilberry complex extract of the present invention was evaluated as follows.

The complex extract of Example 5 mixed with purified water and purified water, and a solution (control group) similar in color to the complex extract of Example 5 by adding a pigment to the purified water were placed in clear glass bottles, respectively, at a wavelength of 440-450 nm. Photographs were taken after irradiating blue light using an LED-Blue light lamp. The results are shown in FIG.

As shown in the results of Figure 1, compared to the purified water or the control compared to the ultra high pressure pretreatment after the subcritical extraction prepared according to the fifth embodiment of the present invention, the chickweed, sunflower and bilberry complex extract greatly reduced the blue light permeation blue light blocking activity It could be confirmed that having.

Example 6: Preparation of Serum Containing Chickweed, Sunflower and Bilberry Complex Extracts

Serum containing the composite extract of Example 5 was prepared according to the conventional method in the composition and content of Table 7. Since Example 5 showed the best skin aging inhibitory effect by ultraviolet light and blue light through the test example, it was prepared using Example 5.

Raw material Content (% by weight) Compound Extract (Example 5) 1.0 Sorbitan Sesquioleate 0.5 Squalane 10.0 Glyceryl Stearate 1.0 Cetearyl Alcohol 1.5 Glyceryl Stearate / Pig-100 Stearate 1.0 Propylene glycol 3.0 Carboxy Polymer 0.1 Triethanolamine 0.1 Phenoxyethanol Quantity Purified water To 100

Example 7 Preparation of Cream Containing Chickweed, Sunflower, and Bilberry Complex

The cream containing the composite extract of Example 5 was prepared according to a conventional method in the composition and content of Table 8 below.

Raw material Content (% by weight) Compound Extract (Example 5) 1.0 Shea butter 2.0 Sorbitan Sesquioleate 0.8 Squalane 10.0 Dimethicone 3.0 Glyceryl Stearate 1.0 Cetearyl Alcohol 2.0 Glyceryl Stearate / Pig-100 Stearate 1.0 Propylene glycol 3.0 Carboxy Polymer 0.25 Triethanolamine 0.25 Phenoxyethanol Quantity Purified water To 100

Test Example 7: Confirmation of formulation stability

The formulations prepared in Examples 6 and 7 were stored in an opaque glass container in an indoor, refrigerator and incubator kept at room temperature (25 ° C.), refrigerated (4 ° C.) and constant temperature (50 ° C.) for 24 weeks, and Observations (discoloration, deodorization and separation) were made. In addition, the stability was confirmed in the temperature cycling conditions (-5 ~ 45 ℃). The results are shown in Table 9.

Temperature condition Check stability Example 6 Example 7 discoloration Deodorant Separation discoloration Deodorant Separation Room temperature (25 ℃) 0 0 0 0 0 0 Refrigerated (4 ℃) 0 0 0 0 0 0 Constant temperature (50 ℃) 0 0 0 0 0 0 Circulation (-5 ~ 45 ℃) 0 0 0 0 0 0

<Formulation stability grade>

0: no change 1: slight change 2: change 3: extreme change

As shown in Table 9, it was confirmed that the formulations of Examples 6 and 7 were stable and did not exhibit discoloration discoloration and separation under 25 ° C, 4 ° C, and 50 ° C temperature and circulation conditions.

Claims (8)

The mixture prepared by mixing chickweed, sunflower and bilberry in the weight ratio of 1-5: 1-5: 1-5, respectively, was put in a plastic pouch and sealed, and water of 40-50 ° C. and 50-100 MPa was used as a pressure medium. After the ultra-high pressure pretreatment for 60 to 180 minutes, the chickweed, sunflower and bilberry complex extracts are prepared by subcritical extraction for 10 to 30 minutes at 120 to 200 ° C, which is a subcritical condition, and pressure of 0.1 to 15 MPa. Cosmetic composition for inhibiting skin aging due to ultraviolet rays and blue light containing. The cosmetic composition for inhibiting skin aging according to claim 1, wherein the complex extract contains 0.01 to 15% by weight, based on the total weight of the cosmetic composition. delete delete According to claim 1, wherein the cosmetic composition is a cosmetic composition for inhibiting skin aging due to ultraviolet light and blue light, characterized in that it has a blue light blocking effect. (A) mixing the chickweed, sunflower and bilberry at a weight ratio of 1-5: 1-5: 1-5 to prepare a mixture;
(B) a pretreatment step of applying the extraction solvent to the mixture itself or the mixture and then pressurized to ultra high pressure; And
(C) Method of producing a chickweed, sunflower and bilberry complex extract comprising the step of adding for 10 minutes to 30 minutes at 120 ~ 200 ℃, pressure 0.1 ~ 15MPa subcritical conditions by adding an extraction solvent. The method of claim 6, wherein the extraction solvent in step (B) or (C) is water, anhydrous alcohol of 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethylene glycol, propylene glycol, butylene glycol, caprylic At least one selected from the group consisting of capric triglycerides, dimethicone, mineral oil, cyclomethicone, octyldodecanol, cetylethylhexanoate, trirexylhexanoin, isopropyl myristate and vegetable oils Method for producing chickweed, sunflower and bilberry complex extract, characterized in that it is used. The method according to claim 6, wherein the pretreatment of the step (B) is a star flower, characterized in that the mixture is put into a plastic pouch and sealed and pressurized for 120 to 180 minutes with water at 40 ~ 50 ℃, 50 ~ 100MPa conditions, Sunflower and bilberry complex extract.

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