KR102081180B1 - Elastic liposome composition comprising sucrose based surfactant, and cosmetic composition containing the same - Google Patents

Abstract

The present invention provides an elastic liposome composition containing an elastic liposome capable of maintaining a stable state for a long period of time while having excellent deformability, flexibility, skin permeability, and efficiency of capturing active ingredients, wherein the elastic liposome composition includes lecithin, and a sucrose-based surfactant in which sucrose and at least one stearic acid are esterified. In addition, the present invention provides a cosmetic composition excellent in stability and skin permeability by containing the elastic liposome composition.

Description

Elastomeric liposome composition containing sucrose-based surfactant and cosmetic composition containing same {ELASTIC LIPOSOME COMPOSITION COMPRISING SUCROSE BASED SURFACTANT, AND COSMETIC COMPOSITION CONTAINING THE SAME}

The present invention relates to an elastic liposome composition comprising a sucrose-based surfactant and a cosmetic composition containing the same.

Human skin consists of three layers: epidermis, dermis and subcutaneous fat, of which the epidermis is composed of the basal layer, the pole layer, the granular layer and the stratum corneum. The stratum corneum is a structure in which keratinocytes are stacked in layers, and lipid components exist in the empty spaces between the keratinocytes. At this time, the transdermal absorption pathway is reported to be more effective when penetrating the lipid component located between the keratinocytes of the skin rather than penetrating the keratinocytes of the skin directly.

Accordingly, attempts have been made to develop a skin absorption delivery system of an active ingredient using liposomes based on lipid components. In particular, the liposome is absorbed into the skin in a state in which a poorly soluble flavonoid material is collected therein, thereby effectively delivering active ingredients such as poorly soluble and insoluble to the skin. In recent years, the development of cosmetic compositions using liposomes has been actively progressed.

For example, Korean Patent Laid-Open Publication No. 10-2003-0085941 discloses a liposome and a cosmetic composition containing the flavonoids having an effect such as antioxidant, skin aging and the like. However, the conventional liposome disclosed in the patent has the advantage that it is possible to carry a poorly soluble substances therein, there is a problem of low collection efficiency of active ingredients such as poorly soluble substances, low stability of liposomes and low skin permeability of liposomes .

In order to solve the above problems, the utilization efficiency of the elastic liposomes having high collection efficiency of the active ingredient and excellent stability of the liposomes while having elasticity or deformability is increasing. The elastic liposomes are mainly composed of a phospholipid forming a double layer and a surfactant which is an edge activator, and has elasticity and flexibility. That is, the elastic liposomes have variability and can effectively pass through the stratum corneum, so that the skin delivery efficiency of the active ingredient can be more excellent.

Most of the elastic liposomes are phospholipids, surfactants having a single carbon chain such as cholesterol, sodium cholate, span 80, octaoxyethylene laurate ether, or ionic surfactants such as sodium hexadecyl sulfate and cetylpyridinium chloride. Is formed by. For example, Korean Patent Publication No. 10-2018-0013541 discloses an elastic liposome formed of a surfactant such as sodium cholate. However, in the case of the elastic liposome formed of the above-described surfactant, while deformability, flexibility, skin permeability, etc. are partially improved, there is a problem that the stability is remarkably decreased.

Accordingly, there is a need for development of elastic liposomes and cosmetic compositions containing the same, which are excellent in properties such as deformability, flexibility, skin permeability, effective ingredient collection efficiency, and can be maintained for a long time.

Republic of Korea Patent Publication No. 10-2003-0085941 Republic of Korea Patent Publication No. 10-2018-0013541

A first object of the present invention is to provide an elastic liposome composition, which contains elastic liposomes that are excellent in deformability, flexibility, skin permeability, and flavonoid capture efficiency, and which can maintain a stable state for a long time.

The second object of the present invention is to provide a cosmetic composition, which can effectively deliver flavonoids to the skin, by containing the elastic liposome composition, excellent skin improvement effect.

The object of the present invention is not limited to the technical problem as described above, another technical problem can be derived from the following description.

In order to achieve the first object, the present invention is lecithin; And sucrose-based surfactants in which sucrose and at least one stearic acid are esterified; it provides an elastic liposome composition.

The sucrose-based surfactant may include one or more selected from the group consisting of sucrose monostearate, sucrose distearate, and sucrose tristearate.

The elastic liposome composition may include the lecithin and sucrose-based surfactant in a weight ratio of 1: 0.01 to 0.30.

The elastic liposome may have a particle size of 70 ~ 200 nanometers.

The flavonoids may include one or more selected from the group consisting of quercetin, catechin, catechin, apigenin, myricetin, luteolin, and pisetin.

In order to achieve the second object, the present invention is lecithin; And it provides a cosmetic composition comprising an elastic liposome composition comprising; sucrose (Sucrose) and at least one stearic acid (estearic acid) esterified sucrose-based surfactant.

In the elastic liposome composition of the present invention, the elastic liposomes may be excellent in deformability, flexibility, skin permeability and flavonoid capture efficiency, but also in stability.

 In particular, the elastic liposome composition of the present invention contains sucrose-based surfactants in which lecithin and sucrose and at least one stearic acid are esterified in a specific ratio, thereby achieving more deformability, flexibility, skin permeability, flavonoid capture efficiency and stability. have.

In addition, the elastic liposome composition of the present invention, as an elastic portion, by containing sucrose and sucrose-based surfactants esterified with 1 to 3 stearic acid, it is possible to realize more excellent deformation, flexibility, skin permeability, flavonoid capture efficiency and stability have.

As the cosmetic composition of the present invention contains elastic liposomes in which poorly soluble flavonoids are collected, the flavonoids can be effectively delivered to the skin, thereby realizing an excellent skin improvement effect. In this case, the skin improvement effect may mean all skin improvement effects that can be implemented by flavonoids, and may typically mean antioxidant, anti-aging and the like.

1 is a view showing a TEM image of the elastic liposome composition of Comparative Example 1, Example 3, Example 7 and Example 11, according to Experimental Example 1.
2 is a graph showing the amount of quercetin skin permeation by time of Example 3, Example 7, Example 11, Comparative Example 1 and the control (1,3-butylene glycol) in Experimental Example 3.
Figure 3, in Experimental Example 3, after about 12 hours, the quercetin content of the stratum corneum, the quercetin content of the skin (epidermis and dermis) and the quercetin content that passed through the skin in Examples 3, 7 and 11, It is a graph shown by Comparative Example 1 and the control (1,3-butylene glycol).

DETAILED DESCRIPTION Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art may easily implement the present invention. As those skilled in the art would realize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the present invention. In the drawings, parts irrelevant to the description are omitted in order to clearly describe the present invention.

The terms or words used in the specification and claims of the present invention are not limited to the ordinary or dictionary meanings, and the inventors can appropriately define the concept of terms in order to explain their invention in the best way. Based on the principle, it should be interpreted as meaning and concept corresponding to the technical idea of the present invention.

Throughout the specification of the present invention, when a part "includes" a certain component, it means that it can further include other components, without excluding the other components unless otherwise stated. .

In the specification of the present invention, "A and / or B" means A or B, or A and B.

Hereinafter, the present invention has been described in detail, but the present invention is not limited thereto.

The present invention provides an elastic liposome composition.

In one embodiment of the invention lecithin; And sucrose (Sucrose) and at least one stearic acid (stearic acid) is a sucrose-based surfactant ester; comprising, provides an elastic liposome composition excellent in stability and skin permeability. Specifically, in the present embodiment, a composition containing an elastic liposome in which flavonoids are collected, comprising: a phospholipid layer formed of sucrose and at least one stearic acid esterified sucrose-based surfactant and lecithin; And it provides a composition containing an elastic liposome consisting of; flavonoids carried in the phospholipid layer.

 Elastic liposomes of this embodiment may be excellent in deformability, flexibility, skin permeability and flavonoid capture efficiency but excellent stability.

In particular, the elastic liposome composition of the present embodiment contains a sucrose-based surfactant in which lecithin and sucrose and at least one stearic acid are esterified in a specific ratio, thereby achieving more deformability, flexibility, skin permeability, flavonoid capture efficiency and stability. have.

In addition, the elastic liposome composition of the present embodiment, as sulphurous, contains sucrose and a sucrose-based surfactant in which 1 to 3 stearic acids are esterified, thereby achieving more deformability, flexibility, skin permeability, flavonoid capture efficiency, and stability. have.

In more detail, in this embodiment, it was confirmed that there is a difference in the deformation, flexibility, skin permeability, flavonoid capture efficiency and stability of the elastic liposomes depending on the number of chains of stearic acid added to sucrose.

For example, when the sucrose-based surfactant is esterified with sucrose and 1 to 3 stearic acid, it was confirmed that the above effects are improved. In particular, when the sucrose and two stearic acid is esterified, it was confirmed that the above effect is further improved.

Lecithin is a phospholipid containing glycerin phosphoric acid and is one of the main components of the biofilm. Lecithin is high in egg yolk, soybean oil, liver and brain. The lecithin, together with the sucrose-based surfactant of this embodiment, serves to improve the elasticity, flexibility and deformability of the elastic liposome of this embodiment.

The sucrose-based surfactant is an elastic activator and can impart elasticity, flexibility, and deformability to the elastic liposome of this embodiment. In particular, since elastic liposomes are easily deformed in the shape of lipid membranes, unlike regular liposomes, even if the particle size increases, the elastic liposomes can easily pass through the gaps between the narrow stratum corneum. In other words, the elastic liposome can capture a larger amount of flavonoids than the normal liposomes, but can effectively deliver them to the skin.

The sucrose-based surfactants of the present embodiment are those in which sucrose and at least one stearic acid are esterified.

For example, the sucrose-based surfactant of the present embodiment may be an ester compound of sucrose and 1-3 stearic acids. In this embodiment, as the sucrose-based surfactant includes an ester compound of sucrose and 1 to 3 stearic acid, it is possible to further improve the deformation, flexibility, elasticity, flavonoid capture efficiency and stability of the elastic liposomes. In more detail, the sucrose-based surfactant may include one or more selected from the group consisting of sucrose monostearate, sucrose distearate, and sucrose tristearate.

The sucrose monostearate may be monoesterified with sucrose and stearic acid.

The sucrose distearate may be a diester of sucrose and stearic acid.

The sucrose tristearate may be a triester of sucrose and stearic acid.

As another example, the sucrose-based surfactant of the present embodiment may be sucrose distearate, but is not limited thereto. In particular, when the sucrose-based surfactant of the present embodiment is sucrose distearate, the deformability, flexibility, elasticity, flavonoid capture efficiency and stability of the elastic liposomes can be significantly improved.

The elastic liposome composition of the present embodiment may include the lecithin and the sucrose-based surfactant in a weight ratio of 1: 0.01 to 0.30. If the elastic liposome composition of the present embodiment does not include the lecithin and the sucrose-based surfactant in a weight ratio of 1: 0.01 to 0.30, the flexibility, elasticity, deformability, flavonoid capture efficiency, and stability of the elastic liposome may be significantly reduced. Can be.

As another example, the elastic liposome composition of the present embodiment may include the lecithin and the sucrose-based surfactant in a weight ratio of 1: 0.02 to 0.20.

As another example, the elastic liposome composition of the present embodiment may include the lecithin and the sucrose-based surfactant in a weight ratio of 1: 0.05 to 0.15.

As another example, the elastic liposome composition of the present embodiment may include the lecithin and the sucrose-based surfactant in a weight ratio of 1: 0.10 to 0.12. In particular, when the elastic liposome composition of the present embodiment comprises the lecithin and the sucrose-based surfactant in a weight ratio of 1: 0.10 to 0.12, the formation of elastic liposomes having remarkably excellent flexibility, elasticity, deformability, flavonoid capture efficiency and stability It may be possible.

The flavonoids of this embodiment may include one or more selected from the group consisting of quercetin, catechin, apigenin, myricetin, luteolin and fisetin. However, the present invention is not limited thereto.

For example, the flavonoid of this embodiment may be a quercetin, but is not limited thereto.

Flavonoids of this embodiment may be included as 0.00001 to 5.0 parts by weight, 0.0001 to 3.0 parts by weight, 0.001 to 1.0 parts by weight, or 0.01 to 0.1 parts by weight based on 100 parts by weight of the total content of lecithin and surfactant, but is not limited thereto.

If the flavonoid of the present embodiment is included in less than 0.00001 parts by weight based on 100 parts by weight of the total content of lecithin and surfactant, there may be a problem that the skin improvement effect such as the antioxidant effect, anti-aging effect by the flavonoids is insignificant. On the other hand, when the flavonoid of the present embodiment is included in more than 5 parts by weight based on 100 parts by weight of the total content of lecithin and surfactant, the stability, skin permeability and flavonoid capture efficiency of the elastic liposomes may be reduced.

The elastic liposome of this embodiment is formed of the flavonoids, lecithin and sucrose-based surfactants as described above, but may have a particle size of 70 ~ 200 nanometers, but is not limited thereto.

If the elastic liposome of this embodiment has a particle size of less than 70 nanometers, the collection efficiency of the flavonoids is significantly lowered, and properties such as flexibility, elasticity, variability, and the like may be reduced. On the other hand, when the elastic liposome of the present embodiment has a particle size of more than 200 nanometers, stability and skin permeability may be significantly reduced.

For example, the elastic liposomes of this embodiment may have a particle size of 80-180 nanometers.

As another example, the elastic liposomes of this embodiment may have a particle size of 90 ~ 160 nanometers.

As another example, the elastic liposome of this embodiment may have a particle size of 100 ~ 130 nanometers. In particular, when the elastic liposome of the present embodiment has a particle size of 100 ~ 130 nanometers, the collection efficiency of the flavonoids can be improved, but the characteristics such as flexibility, elasticity, variability, stability, skin permeability and the like can be further improved.

The elastic liposome composition of this embodiment includes a sucrose-based surfactant in which flavonoids, lecithin and sucrose and at least one stearic acid are esterified; in particular, iii) lecithin includes ii) sucrose The surfactant includes one or more selected from the group consisting of sucrose monostearate, sucrose distearate, and sucrose tristearate, iii) the lecithin and the sucrose When the surfactant is included in a weight ratio of 1: 0.01 to 0.30, a composition containing an elastic liposome having a particle size of 70 to 200 nanometers and having properties of remarkably excellent elasticity, deformation, flexibility, flavonoid capture efficiency, and stability Can provide.

The elastic liposome composition of the present embodiment may further include known additives such as purified water and an organic solvent.

The elastic liposome composition of this embodiment can be applied to the skin per se, and can be applied to the skin in the form contained in the cosmetic composition.

In addition, the present invention provides a cosmetic composition containing an elastic liposome composition.

Cosmetic composition of the present embodiment is lecithin; And sucrose-based surfactants in which sucrose and at least one stearic acid are esterified; and may include an elastic liposome composition including elastic liposomes in which flavonoids are collected. Specifically, the cosmetic composition of the present embodiment comprises a phospholipid layer formed of sucrose and at least one stearic acid esterified sucrose-based surfactant and lecithin; And it may include an elastic liposome composition containing an elastic liposome consisting of; flavonoids carried in the phospholipid layer.

The cosmetic composition of the present embodiment contains an elastic liposome having excellent deformability, flexibility, skin permeability and flavonoid capture efficiency and excellent stability, thereby effectively delivering the active ingredient to the skin. Accordingly, the skin improvement effect of the cosmetic composition of the present embodiment can be significantly improved.

In particular, the elastic liposome composition of the present embodiment contains a sucrose-based surfactant in which lecithin and sucrose and at least one stearic acid are esterified in a specific ratio, so that the elastic liposome composition has better deformability, flexibility, skin permeability, flavonoid capture efficiency and stability. The formation of liposomes may be possible.

In addition, the elastic liposome composition of the present embodiment, as an elastic portion, contains sucrose and a sucrose-based surfactant in which 1 to 3 stearic acids are esterified, thereby providing elasticity having more deformability, flexibility, skin permeability, flavonoid capture efficiency, and stability. Implementation of liposomes may be possible.

The cosmetic composition of the present embodiment may include 0.0001 to 50% by weight of the elastic liposome composition based on the total weight.

As another example, the cosmetic composition of the present embodiment may include 0.001 to 40% by weight of the elastic liposome composition based on the total weight.

As another example, the cosmetic composition of the present embodiment may include 0.01 to 30% by weight of the elastic liposome composition based on the total weight.

In addition to the above-mentioned elastic liposome composition, the cosmetic composition of the present embodiment may include thickeners, fatty substances, organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, emulsifiers, Further comprising at least one additional component selected from the group consisting of fillers, metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles and residual water Can be.

The cosmetic composition of the present embodiment is a flexible cosmetic, nourishing cosmetic, astringent cosmetic, skin, lotion, essence, ampoule, cream, massage cream, pack, makeup base, BB cream, foundation, powder, sun cream, sun lotion, sun milk, cleansing foam , Cleansing cream, cleansing water, shampoo, rinse, conditioner, hair gel, hair serum, hair essence, body lotion, body wash and body spray may be formed of one formulation selected from, but is not limited thereto.

The cosmetic composition of the present invention includes all of the contents of the elastic liposome composition of the present invention described above.

Hereinafter, the elastic liposome composition of the present invention and the cosmetic composition containing the same will be described in detail through Examples, Comparative Examples, and Experimental Examples. These examples are only for illustrating the present invention, and the scope of the present invention is not to be construed as being limited by these examples.

EXAMPLE

Examples 1-12

In Table 1 [unit: wt%], 0.002 g of quercetin was mixed with 100 g of a mixture of lecithin and sucrose-based surfactant. In this case, in Table 1, the mixed weight ratio of the lecithin and sucrose-based surfactants was summarized in Table 2, and the numerical values in Table 2 were rounded off to four decimal places, and only to the third decimal place.

Subsequently, the mixture of lecithin and sucrose-based surfactant and quercetin were mixed, followed by mixing and dissolving an organic solvent in which methanol and chloroform were mixed at a ratio of 10:10 (v / v%).

Thereafter, the organic solvent was completely removed using a rotary evaporator, a thin lipid film was formed on the wall, and then hydrated with purified water to form an elastic liposome.

The elastic liposomes were put into glass beads, homogenized, and then subjected to ultrasonic crushing for 15 minutes to make the particle size more uniform, followed by removal of the quercetin that was not collected with a 0.45 μm syringe filter (Minisart CA 26 mm, Germany). A composition was obtained.

Example 13

It was prepared in the same manner as in Example 7, except that catechin was used instead of quercetin.

Example 14

It was prepared in the same manner as in Example 7, except that apigenin was used instead of quercetin.

Example 15

Except for using mycetin in place of quercetin, it was prepared in the same manner as in Example 7.

Example 16

It was prepared in the same manner as in Example 7, except that luteolin was used in place of quercetin.

Example 17

It was prepared in the same manner as in Example 7, except that phycetin was used instead of quercetin.

Comparative Example

Comparative Examples 1 to 3

Lecithin and sucrose monostearate was prepared in the same manner as in Example 1 except that the mixture was used in a weight ratio of Table 3 [unit: weight ratio].

Comparative Examples 4 to 6

It was prepared in the same manner as in Example 1 except that the surfactant of Table 4 was used instead of sucrose monostearate.

Experimental Example

Experimental Example 1 Measurement of Physical Properties

In the elastic liposome compositions according to Examples 1 to 17 and Comparative Examples 1 to 6, the particle size (nm), zeta potential (mV), encapsulation efficiency (%) and variability of quercetin of the elastic liposomes ( Deformability Index) was evaluated and the results are shown in Table 5.

In this case, the physical properties were measured according to the following method.

(1) Particle size and zeta potential : Measured using Microtrac Flex (Microtrac, USA) using light scattering. The average particle size was represented by cumulative analysis and the distribution was analyzed by the contin method. At this time, the measurement conditions of the particle size and the zeta potential are as follows.

-Temperature: 25 ℃, Fall angle: 165 °, Light source: Argon laser

(2) Collection efficiency of quercetin : A certain amount of the elastic liposome composition of Examples 1 to 17 or Comparative Examples 1 to 6 was taken, and then the quercetin not collected by 0.45 μm syringe filter (Minisart CA 26 mm, Germany) was removed. The same operation was repeated three times to remove uncaptured quercetin. 1 mL of the uncaptured quercetin-free elastic liposome composition was taken, and 10 mL of ethanol was mixed therein to destroy the lipid membrane of the liposome.

Thereafter, the solvent was evaporated using a rotary evaporator, and then 1 mL of ethanol was added thereto, and the quercetin collected in the elastic liposome was quantitatively analyzed by UV-vis spectrometer. At this time, the bath temperature of the rotary evaporator was maintained at about 35 ℃.

In addition, a calibration curve of quercetin for each concentration was prepared to calculate the concentration of quercetin collected in liposomes, and the 'quusetin collection efficiency of elastic liposomes' was calculated by Equation 1. Meanwhile, in Examples 13 to 17, the collection efficiency of catechin, apigenin, myricetin, luteolin and pisetin was measured and calculated in place of quercetin, and the results are shown in Table 5.

[Equation 1] Loading efficiency (%) = (T-P) / T Х 100

T: first quercetin concentration

P: Quercetin concentration that did not pass through 0.45 ㎛ syringe filter

(3) Variable formation : The extent of elastic liposomes passing through the artificial permeation barrier was measured by mini extruder. In more detail, 0.2 MPa pressure was applied to the elastic liposomes for 1 minute and 30 seconds, and then the amount of elastic liposomes that passed through the polycarbonate membrane having pores of 0.08 μm in size was measured and the particle size of the liposomes passed therethrough was measured. . At this time, the elasticity of the elastic liposome membrane is proportional to Equation 2 below.

Equation 2 Deformability index = J _Flux X (r _v / r _p ) ²

J _Flux : The amount of elastic liposomes that has passed through the membrane

r _v : particle size of liposome after extrusion

r _p : pore size of membrane

Looking at Table 5, it can be seen that the elastic liposomes of this embodiment are formed to a particle size of 70 ~ 200 nanometers.

In addition, it can be seen that the elastic liposome of this embodiment has a zeta potential similar to or lower than that of Comparative Example 1 corresponding to general liposomes. This means that the elastic liposomes of this embodiment have a state similar to or more stable than normal liposomes.

In addition, it can be seen that the elastic liposomes of this embodiment show a higher 'quacetin capture efficiency (%)' than Comparative Examples 1 to 6. In particular, when comparing Examples 1 to 12, it can be seen that the inclusion efficiency of the quercetin is more excellent when the sucrose-based surfactant is included as sucrose distearate. In addition, among Examples 4 to 8, when the mixing weight ratio of lecithin and sucrose distearate is 1: 0.111, it can be seen that the quercetin collection efficiency is the best.

In addition, it can be seen that the elastic liposomes of this example show higher variability than Comparative Examples 1 to 6. In particular, when comparing the Examples 1 to 12, it can be seen that when the sucrose-based surfactant is included in sucrose distearate, the variability is more excellent. Also, in Examples 4 to 8, when the mixing weight ratio of lecithin and sucrose distearate is 1: 0.111, it can be seen that variability is the most efficient.

Additionally, in comparison with Example 7 and Examples 13 to 17, in addition to quercetin, even when other types of flavonoid-based materials such as catechin, apigenin, myricetin, luteolin, and phycetin were used, the elastic liposomes were 70 to 200 nanometers. It can be seen that it is formed with a particle size of meters and maintains properties such as low zeta potential, good active ingredient collection efficiency (%), and good variability.

In addition, the transmission electron microscopy (TEM) images of the elastic liposome compositions of (A) Comparative Example 1, (B) Example 3, (C) Example 7 and (D) Example 11 are shown in FIG. 1.

Referring to Figure 1, it can be seen that the elastic liposome of this embodiment has a particle size of about 70 ~ 200 nanometers.

Experimental Example 2 Evaluation of Stability

The stability of Examples 1-12 and Comparative Examples 1-6 was evaluated, and the result is shown in Table 6 and Table 7.

(1) Storage stability : First, compare the particle size immediately after the preparation of the elastic liposome composition with the particle size (average value, unit: nanometer) after storing the elastic liposome composition at about 4 ° C. for 12 weeks, and as a result, Is shown in Table 6. At this time, the particle size was measured by the same method as in Experiment 1.

Looking at Table 6, in the case of the elastic liposome according to this embodiment, it can be seen that the overall stability is superior to the comparative example.

However, in the case of Examples 4 and 12 including the lecithin and sucrose-based surfactant in a weight ratio of 1: 0.250 in the elastic liposome according to the present embodiment, it can be seen that the stable state of the elastic liposome is not maintained. That is, when the lecithin and sucrose-based surfactant is included in a weight ratio of 1: 0.02 to 0.20, it means that the formation of an elastic liposome in a more stable state is possible.

In addition, in the case of the elastic liposomes and general liposomes according to the present embodiment, it can be confirmed that they have a similar level of stability.

(2) Accelerated Stability : Accelerated stability tests were performed with LUMiSizer (using space and time-resolved extinction profiles [STEP] technology). The instability of each elastic liposome was confirmed by measuring a light transmittance, and the results are shown in Table 7 as an instability index. In this case, the instability index has a value between 0 and 1, 0 means a stable state with no change in light transmittance, and 1 means an unstable state that is completely phase separated.

In the accelerated stability test, the elastic liposome composition was stressed at 30 ° C. at about 1,500 rpm for 12 hours, and then the light transmittance was measured at an interval of 60 seconds to determine its instability index.

Looking at Table 7, it can be seen that the acceleration stability of the elastic liposome as a whole compared to the comparative example is excellent.

However, in Examples 4, 8 and 12, which include a lecithin and sucrose-based surfactant in a weight ratio of 1: 0.250 in the elastic liposome according to the present embodiment, it can be seen that the acceleration stability of the elastic liposome is low. . That is, when the lecithin and sucrose-based surfactant is included in a weight ratio of 1: 0.02 to 0.20, it means that the formation of an elastic liposome in a more stable state is possible.

In addition, in the case of the elastic liposomes and general liposomes according to the present embodiment, it can be confirmed that they have a similar level of acceleration stability.

Experimental Example 3 Evaluation of Skin Permeability

The skin permeability of Examples 1-12 and Comparative Examples 1-6 was evaluated, and the result is shown in Table 8.

Skin permeability was evaluated by Franz diffusion cell, and the amount of quercetin per skin area was measured for 0 to 12 hours, and the cumulative skin permeation amount of the final quercetin after about 12 hours was measured. At this time, the skin penetration test conditions are as follows.

λmax (nm): 258

-Detecting: UV-spectrometer

Calibration curve: y = 0.0585x + 0.013

Solvent: Recepter phase

Tilt: 0.0585

y-intercept: 0.013

Hard epidermis area (cm ² ): 0.6362

-Sample amount applied per unit area (μg / cm ² ): 30.40

Sample volume (formulation): 0.2 mL, 20 μg, standard formulation concentration (0.001 g / 10 mL)

-Permeate solution (0h ~ 12h): 0.3mL sample from 5mL

Tape, Skin solution: 7 mL EtOH

In addition, the experiment was conducted with the addition of the case where 1,3-butylene glycol was used in place of the elastic liposome as a control.

When Table 8 is used according to this example, it can be seen that compared to Comparative Examples 1 to 6 and the control group, the skin permeability of the active ingredient is significantly improved.

In particular, Examples 1 to 12 corresponding to the elastic liposome according to the present Example and Comparative Example 1, which is an existing liposome, in the case of the elastic liposome than the conventional liposome, the skin permeation amount of the active ingredient is significantly higher, which is It means that the skin permeability of the following elastic liposome is remarkably excellent.

On the other hand, Examples 1 to 12 according to the present Example is excellent in skin permeability compared to the comparative example, Example 4, Example 8 and Example does not contain a lecithin and sucrose-based surfactant in a weight ratio of 1: 0.02 ~ 0.20 In the case of Example 12, it can be seen that the skin permeability is slightly reduced.

In addition, 1,3-butylene glycol corresponding to the control group and the changes in the quercetin skin permeation amount of Comparative Examples 1, 3, 7 and 11 with time are shown in FIG. 2.

Referring to Figure 2, it can be seen that in Examples 3, 7 and 11 including the elastic liposomes prepared according to the present embodiment, the skin permeation efficiency is significantly superior to that of Comparative Example 1, which is a general liposome. In particular, among Examples 3, 7 and 11, it can be seen that the skin permeation efficiency of Example 7 including sucrose distearate as a sucrose-based surfactant is remarkably excellent.

In addition, after about 12 hours, the content of permeated quercetin was quantified to determine the content (%), which was present in the stratum corneum (Tape), in the skin (epidermis and dermis), and The result is shown in FIG. 3 by dividing the amount through the skin (Transdermal).

3, it can be seen that the total quercetin permeation amount is significantly higher in Examples 3, 7 and 11 including elastic liposomes than in Comparative Example 1 including control or normal liposomes. That is, it can be seen that the skin permeability of the flavonoids can be significantly increased when the elastic liposomes are applied, rather than the general liposomes or simple solvents.

In addition, as shown in Figure 2, in Examples 3, 7 and 11, it can be seen that the quercerine content of Example 7 is significantly high, two stearic acid added in the ester compound of sucrose and stearic acid When sucrose distearate is used, skin permeability is remarkably improved.

As described above, the description of the present invention is for the purpose of illustration, and those skilled in the art to which the present invention belongs may easily change to other specific forms without changing the technical spirit or essential features of the present invention. I can understand. Therefore, it is to be understood that the embodiments described above are exemplary in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present invention is shown by the following claims rather than the detailed description, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention. do

Claims (6)

lecithin; And
An elastic liposome composition comprising a sucrose-based surfactant in which sucrose and at least one stearic acid are esterified;
The elastic liposome composition is
The lecithin and the sucrose-based surfactant, characterized in that it comprises a weight ratio of 1: 0.02 ~ 0.20, elastic liposome composition.
The method of claim 1,
The sucrose-based surfactant
Sucrose monostearate, sucrose distearate, and sucrose tristearate, characterized in that it comprises one or more selected from the group consisting of sucrose tristearate, elastic liposome composition.
delete The method of claim 1,
The elastic liposome is
An elastic liposome composition, characterized in that the particle size is 70 to 200 nanometers.
The method of claim 1,
The elastic liposome composition further comprises a flavonoid,
The flavonoids are characterized in that it comprises one or more selected from the group consisting of quercetin (Quercetin), catechin (Catechin), apigenin (Apigenin), myricetin (Myricetin), luteolin (Luteolin) and pisetin (Fisetin) Elastic liposome composition.
Cosmetic composition containing the elastic liposome composition of claim 1.

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