KR101997023B1 - Hydrangea macrophylla extract mixed composition for relaxation and reducing concentration and uses thereof - Google Patents

Abstract

The present invention relates to a Hydrangea macrophylla extract-mixed composition for concentration and tension relief, a health functional food for concentration and tension relief containing the Hydrangea macrophylla extract-mixed composition, a method for manufacturing the Hydrangea macrophylla extract-mixed composition showing effectiveness in concentration and tension relief, and a Hydrangea macrophylla extract mixture showing effectiveness in concentration and tension relief prepared by the method. The Hydrangea macrophylla extract-mixed composition induces a decrease in a relative high beta (RHB) power spectrum in the left prefrontal lobe, when a user takes the Hydrangea macrophylla extract mixture, thereby exhibiting effectiveness in concentration and tension relief.

Description

Hydrangea extract blend composition for tension and intensive alleviation and use thereof -

The present invention relates to a hydrangea extract mixed composition for tense and concentration alleviation, a health functional food for stress and intensive alleviation comprising the hydrangea extract mixed composition, a method for producing a hydrangea extract mixture having a tension and concentration alleviating effect, And an extracting mixture of hydrangeas having a concentration alleviating effect.

EEG is a rhythmic electrical phenomenon caused by the interaction between the nervous cells of the human brain, which is widely spread on the scalp through the tissues covering the surface of the cerebral cortex, the skull, and the scalp. Generally, in the human cerebral cortex, Potential fluctuations are always occurring. Electroencephalograms (EEGs) can be measured by attaching electrodes on the scalp and inputting electrical signals generated by the brain into the brain waves to record the potentials over time. do.

EEG is able to record temporal and spatial changes of brain activity changing instantaneously, and it has the merit of being able to easily measure brain function non-invasively, consecutively, so that it can be used for brain activity record, diagnosis of disease, sleep study, Science, medicine, and medicine.

Human brain waves are divided into delta waves (0.5 to 3 Hz), theta waves (4 to 7 Hz), alpha waves (8 to 13 Hz), beta waves (14 to 30 Hz) and gamma waves (30 Hz and more). In EEG, alpha waves are expressed as fundamental waves, frequency is 8 ~ 13Hz, amplitude is 10 ~ 150V, and it is seen in normal adult arousal, stable, and closed state. Alpha waves are divided into slow and fast (7 ~ 8Hz), midal (9 ~ 11Hz) and fast (12 ~ 13Hz) depending on frequency.

Slow Alpha waves occur when the brain is concentrated while being relaxed, falling asleep, or resting. In other words, it is focused on rest, but it can be said that it is not stopping the activity, but maintaining the state that can act at any time while supplying energy.

The Mid Alpha is characterized by the usual concentration of accidents and does not involve tension, and is known to occur when meditation, creative activity, or efficient injection of new information or knowledge into the brain.

Fast Alpha Wave It appears when you are extremely nervous and focused on something. The brain keeps repeating, concentrating on it, can not afford to think of anything else, and does not respond to other signals or stimuli.

Beta waves are fast waves of 14 to 30 Hz, have low amplitudes of 5 to 10 V, appear when doing normal work or conscious actions, and are activated when there is mental activity such as information processing.

It is divided into slow beta waves (12-15Hz), mid beta waves (15-20Hz) and high beta waves (20-30Hz) depending on the frequency domain. The Slow beta wave appears when performing a relatively simple task of paying attention while minimizing the activity of the motor sensory cortex, which is a sensory motor rhythm (SMR) paradigm.

Mid Beta waves appear when you perform a relatively mental stressful activity while focusing on a specific topic, such as calculations or mental arithmetic. High Beta waves perform relatively complicated reasoning or complex information processing activities with high mental load, or become predominant in emotional tension, anxiety, and excitement. When you are in normal tension with proper tension, you usually get a beta wave, which works effectively, but when you are stressed and over-excited by tension, you get high beta waves.

Hydrangea (Scientific name Hydrangea macrophylla var. Thunbergii) is a deciduous shrub of a dicotyledonous roe-mallow. Its stem is about 70 cm high and has sweetness on leaves. Flowers bloom in early summer, initially blue, then red, and the center is bisexual. The periphery is surrounded by ornaments with calyx. It grows much in Gangneung alpine region of Gangwon-do, 700m above sea level, and it is cultivated in Gyeongsangnam-do region. Fresh leaves contain phyllodulcin in the form of 8-beta-glucoside, which is not sweet but has been reported to be decomposed by enzymatic action in the drying process to produce a sweet taste. Pillow two gins are characterized by a soft sweetness and a soft peppermint.

Hydrangenic acid, thunberginol and hydrangenol 4'-O-glucoside in hydrangea are antimicrobial and anti-allergic to oral bacteria, It is known that it is effective for diabetic effect. Hydrone is a plant listed in food raw material of food and drug safety department. Because leaf can be used for food, it is safe food raw material that can be used without special toxicity test. Lt; / RTI >

Korean Patent No. 1492074 discloses a composition for skin whitening comprising an extract of hydrangea as an active ingredient and Korean Patent No. 1719162 discloses a composition for the prevention and treatment of alcoholic psychiatric disorders containing hydrangea extract, And is different from the hydroentangling mixed composition for relaxation and concentration alleviation of the present invention.

As a result of analyzing EEG changes using the hydrangea extract compound composition, the inventors of the present invention found that the hydrangea extract mixed composition of the present invention can be applied to the left prefrontal cortex of RHB (Relative High Beta Power Spectrum) , Thereby completing the present invention.

In order to solve the above-described problems, the present invention provides a pharmaceutical composition comprising as an active ingredient a mixture of Hydrangea macrophylla extract powder, crystalline cellulose, lactose, magnesium stearate, silicon dioxide, hydroxypropyl methylcellulose, caramel colorant, titanium dioxide and glycerin The present invention provides a hydrangea extract blending composition for relaxation and concentration alleviation which comprises

In addition, the present invention provides a health functional food for stress and concentration alleviation comprising the hydrated extractive mixed composition.

The present invention also relates to a method for preparing a hydrangea macrophylla extract, comprising the steps of: (a) adding a spirit to a hydrangea tea extract, filtering, concentrating and drying the hydrangea extract to prepare Hydrangea macrophylla extract powder; And (b) mixing the hydrated extract powder prepared in the step (a) with crystalline cellulose, lactose, magnesium stearate, silicon dioxide, hydroxypropylmethyl cellulose, caramel colorant, titanium dioxide and glycerin Wherein the hydrangea extract mixture has a stress and concentration alleviating effect.

In addition, the present invention provides a hydrangea extract mixture having the stress and concentration alleviating effect produced by the above method.

The hydronephritic extraction mixed composition of the present invention can induce reduction of Relative High Beta Power Spectrum (RHB) in the left prefrontal cortex through ingestion of hydrangea extract mixture to obtain a tension and concentration relaxation effect. In addition, the hydrangea extract mixed composition of the present invention can be added to food to be used for the development of a health functional food which induces relaxation and concentration relaxation.

FIG. 1 shows the results of HPLC analysis of the content of thunberginol C in the hydrangea extract powder.
FIG. 2 is a photograph showing a 10-20 international standard electrode attaching method showing a region where an electrode is attached to a human brain for EEG measurement.
FIG. 3 is a photograph showing an EGG cap placed on a subject's brain and measuring brain waves.

In order to achieve the object of the present invention, the present invention provides a method for producing a mixture of hydrangea macrophylla extract powder, crystalline cellulose, lactose, magnesium stearate, silicon dioxide, hydroxypropyl methylcellulose, caramel colorant, titanium dioxide and glycerin As a component of the hydrangea extract mixture composition for relaxation and concentration relaxation.

In the hydrangea extract mixing composition of the present invention, the mixture is added specifically to the hydrangea tea at a rate of 8 to 12 times (v / w) at 60 to 70% (v / v) , 48 to 52% by weight of hydrangea macrophylla extract powder, 1 to 3 times of filtration, concentrated and dried, 40 to 45% by weight of crystalline cellulose, 1.8 to 2.2% by weight of lactose, 0.6 to 0.9% by weight of magnesium stearate, The mixture may be a mixture of 0.6 to 0.9 wt% of hydroxypropylmethylcellulose, 1.3 to 1.7 wt% of hydroxypropylmethylcellulose, 1.3 to 1.7 wt% of caramel colorant, 0.5 to 0.7 wt% of titanium dioxide and 0.1 to 0.3 wt% of glycerin, (V / v) of 65% (v / v) alcohol was added to the hydrangea tea, and the mixture was extracted twice at 60 ° C for 2 hours, then filtered and concentrated to obtain 50 wt.% Hydrangea macrophylla extract powder, 42.7% by weight of cellulose, 2% by weight of lactose, 0.75% by weight of magnesium stearate, 0.75% by weight of silicon dioxide, 1.5% by weight of ropil methyl cellulose, 1.5% by weight of caramel colorant, 0.6% by weight of titanium dioxide and 0.2% by weight of glycerin.

The hydronephritic mixed composition of the present invention is characterized by exhibiting a relaxation and concentration-reducing effect by inducing a reduction of RHB (Relative High Beta Power Spectrum) in the left prefrontal cortex.

RHB refers to the proportion of high beta waves in the whole, where beta waves appear when doing normal work or conscious behavior and are activated when there is mental activity, so the reduction of beta waves stabilizes and relaxes the brain state, .

The present invention also provides a health functional food for stress and concentration alleviation comprising the hydrated extractive mixed composition.

The health functional food may contain various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, colorants and enhancers (cheese, chocolate etc.), pectic acid and its salts, alginic acid and its salts, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. It may also contain flesh for the production of natural fruit juices and vegetable drinks. These components may be used independently or in combination.

The health functional food of the present invention may contain various flavors or natural carbohydrates as an additional ingredient. The natural carbohydrates are sugar saccharides such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like.

The present invention also relates to

(a) 60 to 70% (v / v) alcohol was added to the water tea at 8 to 12 times (v / w) and then extracted 1 to 3 times at 50 to 70 ° C for 1 to 3 hours. Preparing Hydrangea macrophylla extract powder; And

(b) 48 to 52% by weight of hydrangea extract powder prepared in the step (a), 40 to 45% by weight of crystalline cellulose, 1.8 to 2.2% by weight of lactose, 0.6 to 0.9% by weight of magnesium stearate, Comprising mixing 0.6 to 0.9% by weight of silicon dioxide, 1.3 to 1.7% by weight of hydroxypropylmethylcellulose, 1.3 to 1.7% by weight of caramel dyes, 0.5 to 0.7% by weight of titanium dioxide and 0.1 to 0.3% by weight of glycerin, Provided is a method for producing a hydrangea extract mixture having a concentrated relaxation effect.

The method for producing the hydrangea extract mixture of the present invention having the effect of tautness and concentration alleviation, more specifically,

(a) 65% to hydrangea tea (v / v) to 10 times ethanol (v / w) was added and then extracted twice with 60 ℃ for 2 hours, filtered and concentrated and dried hydrangea (Hydrangea macrophylla) preparing an extract powder ; And

(b) extracting hydrangea powder comprising 50% by weight of the hydrated extract powder prepared in the step (a), 42.7% by weight of crystalline cellulose, 2% by weight lactose, 0.75% by weight magnesium stearate, 0.75% by weight silicon dioxide, 1.5% by weight of propyl methylcellulose, 1.5% by weight of caramel colorant, 0.6% by weight of titanium dioxide and 0.2% by weight of glycerin.

The present invention also provides a hydrangea extract mixture having the tautness and concentration alleviating effect produced by the above method.

Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Manufacturing example  1. Manufacture of hydrangea extract powder

Hydrangea macrophylla leaves were dried in the sunlight in July and August, the water content of each part of hydrangea leaves was uniformed, the cell tissue was properly destroyed, and hydrangea Was used as a process to make the green leaf in a dark brown color by using an observing machine. The noted hydrangea leaves were sealed in a sealed container, aged at 37 캜 for 12 hours, and dried thoroughly in the sun to prepare hydrangea tea.

1 kg of the hydrangea tea prepared above was pulverized, 10 L of 65% ethanol was added thereto, and the mixture was extracted twice at 60 ° C for 2 hours, and then filtered under reduced pressure using a filter paper (pore 0.7 micrometer). The filtrate was concentrated at 50 to < RTI ID = 0.0 > 18 < / RTI > Dextrin was dissolved in the concentrate in a 1: 1 weight ratio with the solid content of the concentrate, followed by spray drying.

When the hydronephric extract powder prepared above was dissolved in methanol and analyzed by HPLC, it was confirmed that the active ingredient, thunberginol C, was 0.02% or more (Fig. 1).

Manufacturing example  2. Manufacture of hydrangea extract mixture

A mixture of 50% by weight of the hydrated extract powder obtained in Preparation Example 1, 42.7% by weight of crystalline cellulose, 2% by weight lactose, 0.75% by weight magnesium stearate, 0.75% by weight silicon dioxide, 1.5% by weight hydroxypropylmethylcellulose, 1.5% by weight caramel colorant, 0.6% by weight of titanium dioxide and 0.2% by weight of glycerin were mixed to prepare a hydrangea extract mixture.

Comparative Example  1. Hydrone extract mixture

(a) a mixture of a brown rice embryo and a fructooligosaccharide in a weight ratio of 94: 5 and then sterilized at 100 ° C for 10 to 30 minutes was cooled to 36 ° C or lower, and then cooled to an Aspergillus oryzae were mixed in a weight ratio of 99: 1, sterilized water was added, and the mixture was incubated at 36 ° C. and 60% relative humidity for 48 to 96 hours. The cultured cultures were dried at 45 ° C for 6 to 10 hours, and dried to a moisture content of 8% (v / w) or less to prepare brown rice embryo enzymes.

(b) hydrangea blend was prepared by mixing 25% by weight of hydrangea leaves, 20% by weight of Angelica gigas, 20% by weight of the brown rice embryo enzyme prepared in step (a), 20% by weight of gold, 10% by weight of omija and 5% Respectively.

(c) The hydrangea mixture of step (b) was added to the extract and water was added 10 times by weight of the mixture, and the mixture was extracted at a temperature of 100 ° C. and a pressure of 1 to 2 kgf / cm ² for 12 hours. The extracted extract was filtered with a 5 탆 filter, and the filtrate was concentrated to a solid content of 20% (w / v) at 60 캜 by using a vacuum concentrator. The concentrated concentrate was pulverized using a spray drier (hot air temperature 180 占 폚, air temperature 103 占 폚, disk speed 10,000 rpm) to prepare hydrangea extract powder.

(d) the hydrolyzate extract powder prepared in the step (c), 35 wt% of fructooligosaccharide, 30 wt% of maltodextrin, 5 wt% of inositol, 2 wt% of taurine, 1% by weight of vitamin B1, 0.01% by weight of vitamin B1, 0.01% by weight of vitamin B3, 0.01% by weight of vitamin B5 and 0.01% by weight of vitamin B6.

Comparative Example  2. Mountain landscape  Extraction mixture

The extract mixture was prepared by the method of Preparation Example 2, except that hydrangea macrophylla was not used, and Hydrangea serrata was used to prepare an extract of a mountain water soup.

Example  1. Effects of extractive hydration mixture on EEG

The brain waves were measured in order to know how the intake of the hydrangea extract mixture of Preparation Example 2 affected human brain activity. EEG measurements were performed on a total of 20 subjects, 10 of each male and female, in a laboratory of 32.4 m ^{2 in} which the noise was not mixed and the temperature was maintained at 23 ° C and 50% humidity. The EEG used for the EEG was a QEEG-64FX system (LAXTHA Inc. Korea). EEG signals were measured using LAXTHA's data collection equipment and analysis program (Telescan). The left and right prefrontal cords (Fp1, Fp2, Fpz, Af3, Af4, Afz), left and right frontal lobes (F3, F4, F7, F8, Fz, Fc1, Fc2, Left and right temporal lobes (P3, P4, P7, P8, Cp1, Cp2, Cz, Pz), left and right temporal lobes (T3, T4, T7, T8, Cp5, Cp6) O1, O2, and Oz), and ground and reference electrodes were attached to the right and left earlobes, respectively (FIGS. 2 and 3). Subjects were examined for 45 seconds with eyes closed (no irritation) and with hydrangea extract (dietary stimulation).

The data processing obtained by the EEG extraction of the hydronephritic extract mixture was performed by extracting only a representative 30-second EEG signal among the EEG signals of the subject and performing an EEG batch processing. Then, the statistical analysis software program SPSS (SPSS 23.0 version ) Was used to perform a corresponding sample T test.

Table 1 shows the types of EEG indices for data analysis of hydronephrine extract mixtures, and Table 2 shows the meanings of EEG indices.

Types of EEG indicators used in data analysis No. Analysis index Full name of EEG indicator Waveband (Hz) One AT Absolute Theta Power Spectrum 4 to 8 2 AA Absolute Alpha Power Spectrum 8-13 3 AB Absolute Beta Power Spectrum 13 to 30 4 AG Absolute Gamma Power Spectrum 30 to 50 5 AFA Absolute Fast Alpha Power Spectrum 11-13 6 ASA Absolute Slow Alpha Power Spectrum 8 ~ 11 7 ALB Absolute Low Beta Power Spectrum 12 to 15 8 AMB Absolute Mid Beta Power Spectrum 15-20 9 AHB Absolute High Beta Power Spectrum 20 ~ 30 10 RT Relative Theta Power Spectrum (4 to 8) / (4 to 50) 11 RA Relative Alpha Power Spectrum (8 to 13) / (4 to 50) 12 RB Relative Beta Power Spectrum (13 to 30) / (4 to 50) 13 RG Relative Gamma Power Spectrum (30 to 50) / (4 to 50) 14 RFA Relative Fast Alpha Power Spectrum (11 to 13) / (4 to 50) 15 RSA Relative Slow Alpha Power Spectrum (8 to 11) / (4 to 50) 16 RLB Relative Low Beta Power Spectrum (12 to 15) / (4 to 50) 17 RMB Relative Mid Beta Power Spectrum (15 to 20) / (4 to 50) 18 RHB Relative High Beta Power Spectrum (20 to 30) / (4 to 50) 19 RST Ratio of SMR to Theta (12 to 15) / (4 to 8) 20 RMT Ratio of Mid Beta to Theta (15 to 20) / (4 to 8) 21 RSMT Ratio of (SMR-Mid Beta) to Theta (12 to 20) / (4 to 8) 22 RAHB Ratio of Alpha to High Beta (8 to 13) / (20 to 30) 23 SEF50 Spectral Edge Frequency 50% 4 to 50 24 SEF 90 Spectral Edge Frequency 90% 4 to 50 25 ASEF Spectral Edge Frequency 50% of Alpha Spectrum Band 8-13

Meaning of EEG indices used in data analysis No. Analysis index meaning One AT Absolute power of theta wave (meditation, sleepy) 2 AA Absolute power of Alpha wave (relaxation, stability) 3 AB The absolute power of Beta waves (tension, arousal, activity, cognitive function) 4 AG Absolute power of the Gamma wave (highly cognitive or anxious, excited) 5 AFA Fast alpha Absolute power of wave (comfortable and immersive) 6 ASA Absolute power of Slow alpha wave (relaxation) 7 ALB Absolute power of the low beta wave, SMR parameter (caution or boundary) 8 AMB Mid beta wave absolute power (concentration, attention) 9 AHB High beta Absolute power (stress, mental load, tension) 10 RT Percentage of theta waves in total 11 RA The ratio of Alpha waves in total 12 RB The ratio of Beta waves in total 13 RG Percentage of Gamma waves in total 14 RFA The percentage of Fast Alpha waves in total 15 RSA Slow Alpha ratio in all 16 RLB The ratio of Low Beta waves in total 17 RMB Percentage of Mid Beta waves in total 18 RHB The ratio of High Beta waves in total 19 RST The ratio of SMR wave / Theta wave 20 RMT Mid Beta wave / Theta wave ratio 21 RSMT SMR ~ Mid Beta wave / Theta wave (EEG concentration indicator) 22 RAHB Alpha wave / High Beta wave (stable and relaxed) 23 SEF50 Median Frequency Brain activity (EEG) 24 SEF 90 Median Frequency Brain activity (mental stress, stress) 25 ASEF Alpha SEF50 (Comfort: Fast Alpha wave)

In the present embodiment, EEG measurement results showed that there was a statistically significant difference only in RHB (Relative High Beta Power Spectrum) index among 25 EEG analysis indexes ( p <0.05). Since the power spectra of the twenty-four power amplifiers were not significantly different from each other, they were not described in this embodiment.

As a result, it was found that the tasting of the hydrangea extract mixture of the present invention resulted in reducing the relative high beta power spectrum (RHB) at the Fp1 site of the left prefrontal cortex, thereby relieving tension and concentration (Table 3).

Example  2. Of the extraction mixture RHB  Impact on Power Spectrum

The results of observing changes in RHB after the ingestion of the extract mixture of Preparation Example 1 and Comparative Example are shown in Table 4 below. As a result, the hydrangea extract mixture of Preparation Example 1 showed an effect of reducing the Relative High Beta Power Spectrum (RHB) index at the Fp1 site ( p < 0.05), while the extract mixture of Comparative Example 1 and Comparative Example 2 showed all brain sites In the RHB index.

Effect of extraction mixture on RHB power spectrum Brain area Production Example 1 Comparative Example 1 Comparative Example 2 Fp1 Decrease ▼ - - Fp2 - - - F3 - - - F4 - - - T3 - - - T4 - - - P3 - - - P4 - - - O1 - - - O2 - - - F7 - - - F8 - - - T7 - - - T8 - - - P7 - - - P8 - - - Afz - - - Cz - - - Fz - - - Pz - - - Fpz - - - Oz - - - Af3 - - - Af4 - - - Fc1 - - - Fc2 - - - Fc5 - - - Fc6 - - - Cp1 - - - Cp2 - - - Cp5 - - - Cp6 - - -

Fp1 / Af3: Left prefrontal, Fp2 / Af4: Right prefrontal, Fpz / Afz: Central prefrontal, F3 / F7 / Fc1 / Fc5: Left frontal, F4 / T3 / T7 / Cp5: Left temporal, T4 / T8 / Cp6: Right temporal, P3 / T6 / Fc6: Right frontal, Fz: Central frontal, P4 / Cp1: Left parietal, P4 / P8 / Cp2: Right parietal, Cz / Pz: Central parietal, O1: Left occipital, O2: Right occipital (Right parietal) occipital), Oz: central occipital (Central occipital)

Claims (7)

After drying, the hydrangea leaves were sealed in a sealed container, and the mixture was vigorously aged at 37 ° C for 12 hours. The hydrangea tea was added with 65% (v / v) vat 10 times (v / w) 50% by weight of extracted Hydrangea macrophylla powder, 42.7% by weight of crystalline cellulose, 2% by weight of lactose, 0.75% by weight of lactose, magnesium stearate, RHB (Relative High Beta Power) was added to the left prefrontal cortex containing 0.75% by weight of silicon dioxide, 1.5% by weight of hydroxypropylmethylcellulose, 1.5% by weight of caramel colorant, 0.6% by weight of titanium dioxide and 0.2% Spectrum. &Lt; / RTI &gt; delete delete A health functional food for stress and intensive relaxation comprising the hydrated extractive mixed composition according to claim 1. The composition according to claim 4, further comprising at least one compound selected from the group consisting of vitamins, minerals, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, And a carbonating agent used in a carbonated beverage. &Lt; Desc / Clms Page number 24 &gt; delete delete

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