KR101847375B1 - Dandelion mixed extract composition for improving brain comfort when tasting and uses thereof - Google Patents

Abstract

The present invention relates to: a Taraxacum platycarpum mixed extract composition for improving brain comfort; health functional food for improving brain comfort including the Taraxacum platycarpum mixed extract composition; a method for producing the Taraxacum platycarpum mixed extract composition having an effect of improving brain comfort; and to a Taraxacum platycarpum mixed extract produced by the method. The Taraxacum platycarpum mixed extract composition of the present invention, when taking the Taraxacum platycarpum mixed extract, induces the increase of relative fast alpha power spectrum (RFA) in the left prefrontal cortex, left frontal lobe, right frontal lobe, middle larynx, right parietal lobe, central parietal lobe and left parietal lobe, and induces the increase of the spectral edge frequency 50% of the alpha spectrum band (ASEF) in the middle prefrontal cortex to increase the brain comfort.

Description

Dandelion mixed extract composition for enhancing the comfort of brain during tasting and its use -

The present invention is based on an increase in RFA (relative fast alpha power spectrum) and an increase in ASEF (spectral edge frequency 50%) in the middle prefrontal cortex in the left prefrontal cortex, right frontal cortex, left frontal lobe, right frontal lobe, medial parietal lobe, of alpha spectrum band in order to enhance the comfort of the brain, a health functional food for enhancing brain comfort, which comprises the above-mentioned dandelion mixed extract composition, and a dandelion mixed extract having brain-improving effect And a dandelion mixed extract prepared by the above method.

Dandelion is a perennial plant belonging to the family Asteraceae (Compositae). It is a perennial plant belonging to the family Asteraceae ( Taraxacum hallanisanese ), mountain dandelion ( T. ohwianum ), white dandelion ( T. coreanum ), T. officinale , T. mongolicum ) is inhabited (Lee, 1996).

Dandelion is widely used in Korea. Young leaves are used as herbs, and roots are used as medicines. It is used in the treatment of constipation, rheumatism, neurosis, and blindness in Europe, and in Europe, in the treatment of constipation, tuberculosis, epilepsy, It is used as a substitute.

The dandelion leaves are rich in vitamins and minerals, low in fat content and calories, suitable for nutritionally good well-being foods, and contain a lot of taraxin, inulin, and carotenoid, Taraxathin, triterpene taraxerol, taraxasterol, β-sitosterol, and caffeic acid, as well as vitamin A, vitamin C, tocopherol, ), Calcium (Ca), iron (Fe), and potassium (K).

In this study, we investigated the effects of ginseng root extracts on the growth of dendritic cells, liver function promoting and improving effect is excellent, and (Williams et al 1996. Flavonoid, cinnamic acids and coumarins from the different tissues and medicinal preparation of Taraxacum officinale Phytochem 42:... 121-127), dandelion ethanol extract of anti-allergy, anti- Inflammatory, antitumor, and anticancer activities have been reported (Ho et al., 1998. Desacetylmartricarin and anti-allegic components from Taraxacum platycarpum. Planta Med. 64: 577-578).

Recently, a variety of processing products such as dandelion kimchi, dandelion soap, dandelion tea, dandelion fragrance, dandelion extract, dandelion extract, dandelion flower, dandelion chocolate, and dandelion steamed bread have been developed and marketed using the efficacy of dandelion 2017. Yanggu Dandelion Convergence Industry System Project Final Report).

Korean Patent Registration No. 10-1193085 discloses a method for preparing a composition for improving, preventing or treating diabetic or diabetic complication, which comprises a fermented broth of Dandelion Lactobacillus as an active ingredient. However, the composition of the present invention for improving brain comfort, . Korean Patent Registration No. 10-1725665 discloses a method for producing a functional dandelion fermented product by mixed fermentation of a pickle extract of dandelion peria, but there is no description of the use for enhancing brain comfort as in the present invention. Korean Patent No. 10-0870241 discloses a health supplement food based on dandelion and a manufacturing method thereof, but is different from the mixed dandelion extract composition of the present invention for improving brain comfort. Korean Patent No. 10-1438717 discloses a fermented extract composition of a mixed plant of dandelion plants having a hepatocyte protective action and a method for producing the same, but it does not verify brain comfort by measuring EEG of the brain as in the present invention .

As a result of analyzing EEG changes using the dandelion mixed extract composition, the present inventors have found that the dandelion mixed extract composition of the present invention can be applied to the left frontal lobe, the right frontal lobe, the left larynx, By confirming the increase of RFA (relative fast alpha power spectrum) in the central occipital lobe, right parietal lobe, central parietal lobe and left parietal lobe and increase of ASEF (spectral edge frequency 50% of alpha spectrum band) .

In order to solve the above problems, the present invention provides a mixed extract composition of dandelion for improving brain comfort comprising a mixture of fermented dandelion extract, jujube concentrate and Angelica giganteum concentrate as an active ingredient.

In addition, the present invention provides a health functional food for enhancing brain comfort comprising the above-described mixed extract composition of dandelion.

The present invention also relates to a composition comprising (a) a dandelion powder, a fructooligosaccharide powder and an Aspergillus oryzae ), adding water thereto, adding water to the fermented fermented product, and extracting the fermented product to prepare a fermented dandelion extract; And (b) mixing the fermented dandelion extract prepared in step (a) with a jujube concentrate and a Ganoderma concentrate to prepare a mixed extract of dandelion having a brain-cooling enhancing effect.

The present invention also provides a mixed extract of dandelion having the brain-comfort enhancing effect prepared by the above-described method.

The mixed extract composition of dandelion of the present invention showed an increase in relative fast alpha power spectrum (RFA) in the left prefrontal cortex, the right frontal lobe, the left occipital lobe, the right occipital lobe, the central occipital lobe, the central parietal lobe and the left parietal lobe (ASEF) in the central pharyngeal lobe, and the brain enhancement effect can be obtained by inducing an increase in the ASEF (spectral edge frequency 50% of the alpha spectrum band). In addition, the present invention can be used for the development of a health functional food which induces a brain-comfort enhancing effect by adding a dandelion mixed extract composition to a food.

FIG. 1 is a photograph showing a 10-20 international standard electrode attaching method showing a region where an electrode is attached to a human brain for EEG measurement.
FIG. 2 is a photograph showing an EGG cap placed on a subject's brain and measuring brain waves.
FIG. 3 shows a brain map mapping result showing changes in RFA (relative fast alpha power spectrum) after a water tasting (left) and a tasting of dandelion mixed extract (right).
FIG. 4 is a map of the brain map showing changes in the ASEF (spectral edge frequency 50% of the alpha spectrum band) after the water tasting (left) and tasting of the dandelion mixed extract (right).

In order to achieve the object of the present invention, the present invention provides a mixed extract composition of dandelion for improving brain comfort comprising a mixture of a fermented dandelion extract, a jujube concentrate and a ginseng concentrate as an active ingredient.

In the dandelion mixed extract composition for enhancing brain comfort of the present invention, the mixture is preferably a dandelion powder, a fructooligosaccharide powder and an Aspergillus oryzae were mixed at 94 ~ 96: 4 ~ 6: 0.08 ~ 0.12 weight ratio, water was added and the fermented product was fermented at 30 ~ 45 ℃ for 48 ~ 72 hours. , a mixture of 94 to 96% by weight of fermented dandelion extract extracted at 95 to 100 ° C for 8 to 16 hours, 2 to 4% by weight of a jujube concentrate and 1.5 to 2.5% by weight of a concentrate of Angelica keiskei koidz. Specifically, dandelion powder, fructo-oligosaccharide powder, and Aspergillus oryzae were mixed at a weight ratio of 95: 4.9: 0.1, water was added, and water was added 10 times (v / w) to the fermented product fermented at 37 ° C for 48 to 72 hours, 95% by weight of fermented dandelion extract, 3% by weight of jujube concentrate, and 2% by weight of Angelica gigas Lacticum concentrate.

The mixed extract composition of dandelion according to the present invention has an increase in relative fast alpha power spectrum (RFA) in the left prefrontal region, a right frontal lobe, a left frontal lobe, a right frontal lobe, a middle laryngeal lobe, edge frequency 50% of the alpha spectrum band), thereby exhibiting a brain-comfort enhancing effect.

The increase in RFA means that the ratio of fast alpha waves increases in the entire EEG. As the rapid increase in ALPA increases the concentration of the brain by awakening the brain, you can feel the sense of stability and comfort. ASEF means Alpha wave SEF50, and the increase in ASEF means that the rate of Fast Alpha wave increases, and it is used to evaluate comfort level.

In addition, the present invention provides a health functional food for enhancing brain comfort comprising the above-described mixed extract composition of dandelion.

The health functional food may contain various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, colorants and enhancers (cheese, chocolate etc.), pectic acid and its salts, alginic acid and its salts, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. It may also contain flesh for the production of natural fruit juices and vegetable drinks. These components may be used independently or in combination.

The health functional food of the present invention may contain various flavors or natural carbohydrates as an additional ingredient. The natural carbohydrates are sugar saccharides such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like.

The present invention also relates to

(a) Dandelion powder, fructooligosaccharide powder and Aspergillus oryzae strain are mixed in a weight ratio of 94 to 96: 4 to 6: 0.08 to 0.12, water is added, Adding water to the fermented fermented product for 72 hours at a rate of 5 to 20 times (v / w) relative to the fermented product and extracting the fermented dandelion extract at 95 to 100 ° C for 8 to 16 hours; And

(b) mixing 94 to 96% by weight of the fermented dandelion extract prepared in step (a) with 2 to 4% by weight of a jujube concentrate and 1.5 to 2.5% by weight of a ganoderma concentrate, based on the total weight of the dandelion mixed extract liquid A method for producing a dandelion mixed extract having a brain-comfort enhancing effect is provided.

The method for producing a dandelion mixed extract having the brain-comfort enhancing effect of the present invention, more specifically,

(a) Dandelion powder, fructooligosaccharide powder and Aspergillus oryzae strain were mixed in a weight ratio of 95: 4.9: 0.1, water was added, and the resulting fermented product was fermented at 37 DEG C for 48 to 72 hours Adding water 10 times (v / w) to the fermented product, and extracting the fermented dandelion extract at 97 ° C for 12 hours; And

(b) mixing the 95% by weight of the fermented dandelion extract prepared in the step (a) with 3% by weight of the jujube concentrate and 2% by weight of the ginseng concentrate, based on the total weight of the mixed dandelion extract solution.

The present invention also provides a mixed extract of dandelion having a brain-comfort enhancing effect produced by the above-described method.

Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Manufacturing example  1. Dandelion mixed extract

(a) The stem and root parts of the dandelion cultivated in a clean area, Gangwon-do, were harvested and immediately washed, dried and powdered to prepare dandelion powder.

(b) A mixture of 95% by weight of the dandelion powder prepared in the step (a) and 4.9% by weight of fructooligosaccharide powder was sterilized with steam at 110 ° C for 20 minutes using a steam sterilizer, , Aspergillus oryzae ) in an amount of 0.1% by weight was added with sterilized water at a viscosity (10 to 20% based on the mixture) suitable for culturing. The mixture containing the sterilized water was cultured at 37 DEG C and a relative humidity of 60% or more for 48 to 72 hours to prepare a dandelion fermented product. The fermented dandelion extract was prepared by adding water 10 times (v / w) to the fermented product of dandelion prepared above, then extracting it for 12 hours while maintaining the pressure at 1.0 to 2.0 kgf / cm ² at 97 ° C, Respectively.

(c) 95% by weight of the fermented dandelion extract prepared in the step (b), 3% by weight of the jujube concentrate and 2% by weight of the concentrate were mixed with stirring at 90 ° C for 30 minutes to prepare a mixed dandelion extract.

Example  1. Effects of Dandelion Mixture Extract on EEG

We measured the EEG by knowing the influence of the tasting of the dandelion mixed extract on human brain activity. EEG measurements were performed on a total of 20 subjects, 10 of each male and female, in a laboratory of 32.4 m ^{2 in} which the noise was not mixed and the temperature was maintained at 23 ° C and 50% humidity. The brain waves were measured using the QEEG-64FX system (LXE3208, LAXTHA Inc. Korea). The EEG signals of the subjects were measured using LAXTHA's data collection equipment and analysis program (Telescan). The left and right prefrontal cords (Fp1, Fp2, Fpz, Af3, Af4, Afz), left and right frontal lobes (F3, F4, F7, F8, Fz, Fc1, Fc2, Left and right temporal lobes (P3, P4, P7, P8, Cp1, Cp2, Cz, Pz), left and right temporal lobes (T3, T4, T7, T8, Cp5, Cp6) O1, O2, and Oz), and ground and reference electrodes were attached to the right and left earlobes, respectively (FIGS. 1 and 2). Subjects were measured for 45 seconds with a blind tasting (no irritation) and a mixed extract of dandelion (unstimulated) with eyes closed.

The data processing obtained by EEG measurement on the mixed extract of dandelion extracts only the representative 30-second EEG signals among the EEG signals of the subject and performed by batch processing of brain waves. Then, statistical analysis software program SPSS (SPSS 23.0 version ) Was used to perform a corresponding sample T test.

Table 1 shows the types of EEG indicators for analyzing data on the mixed dandelion extracts, and Table 2 shows the meanings of the EEG indices.

Types of EEG indicators used in data analysis No. Analysis index Full name of EEG indicator Waveband (Hz) One AT Absolute Theta Power Spectrum 4 to 8 2 AA Absolute Alpha Power Spectrum 8-13 3 AB Absolute Beta Power Spectrum 13 to 30 4 AG Absolute Gamma Power Spectrum 30 to 50 5 AFA Absolute Fast Alpha Power Spectrum 11-13 6 ASA Absolute Slow Alpha Power Spectrum 8 ~ 11 7 ALB Absolute Low Beta Power Spectrum 12 to 15 8 AMB Absolute Mid Beta Power Spectrum 15-20 9 AHB Absolute High Beta Power Spectrum 20 ~ 30 10 RT Relative Theta Power Spectrum (4 to 8) / (4 to 50) 11 RA Relative Alpha Power Spectrum (8 to 13) / (4 to 50) 12 RB Relative Beta Power Spectrum (13 to 30) / (4 to 50) 13 RG Relative Gamma Power Spectrum (30 to 50) / (4 to 50) 14 RFA Relative Fast Alpha Power Spectrum (11 to 13) / (4 to 50) 15 RSA Relative Slow Alpha Power Spectrum (8 to 11) / (4 to 50) 16 RLB Relative Low Beta Power Spectrum (12 to 15) / (4 to 50) 17 RMB Relative Mid Beta Power Spectrum (15 to 20) / (4 to 50) 18 RHB Relative High Beta Power Spectrum (20 to 30) / (4 to 50) 19 RST Ratio of SMR to Theta (12 to 15) / (4 to 8) 20 RMT Ratio of Mid Beta to Theta (15 to 20) / (4 to 8) 21 RSMT Ratio of (SMR-Mid Beta) to Theta (12 to 20) / (4 to 8) 22 RAHB Ratio of Alpha to High Beta (8 to 13) / (20 to 30) 23 SEF50 Spectral Edge Frequency 50% 4 to 50 24 SEF 90 Spectral Edge Frequency 90% 4 to 50 25 ASEF Spectral Edge Frequency 50% of Alpha Spectrum Band 8-13

Meaning of EEG indices used in data analysis No. Analysis index meaning One AT Absolute power of theta wave (meditation, sleepy) 2 AA Absolute power of Alpha wave (relaxation, stability) 3 AB The absolute power of Beta waves (tension, arousal, activity, cognitive function) 4 AG Absolute power of the Gamma wave (highly cognitive or anxious, excited) 5 AFA Fast alpha Absolute power of wave (comfortable and immersive) 6 ASA Absolute power of Slow alpha wave (relaxation) 7 ALB Absolute power of the low beta wave, SMR parameter (caution or boundary) 8 AMB Mid beta wave absolute power (concentration, attention) 9 AHB High beta Absolute power (stress, mental load, tension) 10 RT Percentage of theta waves in total 11 RA The ratio of Alpha waves in total 12 RB The ratio of Beta waves in total 13 RG Percentage of Gamma waves in total 14 RFA The percentage of Fast Alpha waves in total 15 RSA Slow Alpha ratio in all 16 RLB The ratio of Low Beta waves in total 17 RMB Percentage of Mid Beta waves in total 18 RHB The ratio of High Beta waves in total 19 RST The ratio of SMR wave / Theta wave 20 RMT Mid Beta wave / Theta wave ratio 21 RSMT SMR ~ Mid Beta wave / Theta wave (EEG concentration indicator) 22 RAHB Alpha wave / High Beta wave (stable and relaxed) 23 SEF50 Median Frequency Brain activity (EEG) 24 SEF 90 Median Frequency Brain activity (mental stress, stress) 25 ASEF Alpha SEF50 (Comfort: Fast Alpha wave)

In the present example, EEG data were statistically significant only in 25 EEG (Relative Fast Alpha Power Spectrum) and ASEF (Spectral edge frequency 50% of alpha spectrum band) p < 0.05). The remaining 23 power spectra were not described in this embodiment because no difference was found.

1. Relative fast alpha power spectrum (RFA)

The RFA indicator is the relative power spectrum value divided by the fast alpha wave range (11 ~ 13 Hz) as the absolute power ratio of the fast alpha band to the absolute power of the entire frequency domain in the EEG spectrum. The mean value of RFA, which is an indicator of EEG analysis, increased statistically when tasting mixed dandelion extract ( p <0.05).

2. ASEF (Spectral edge frequency 50% of alpha spectrum band)

The ASEF index is an alpha wave SEF50, which is known to be an EEG indicator indicating comfort. The mean value of ASEF, which is an indicator of EEG analysis, increased significantly during tasting of dandelion mixed extract ( p <0.05).

Based on the above results, it was found that the intake of the mixed dandelion extract of the present invention induces a change in brain waves, thereby exhibiting a brain comfort enhancing effect.

Claims (7)

Dandelion powder, fructooligosaccharide powder and Aspergillus oryzae strain were mixed in a weight ratio of 94 to 96: 4 to 6: 0.08 to 0.12, water was added, and the mixture was stirred at 30 to 45 ° C for 48 to 72 hours 94 to 96% by weight of fermented dandelion extract, 2 to 4% by weight of jujube concentrate, and Angelica keiskei kotatsu (fermented soybean curd) were added to the fermented fermented product at a temperature of 95 to 100 DEG C for 5 to 20 times (v / (Relative fast alpha power spectrum) in the left prefrontal cortex, the right frontal cortex, the left temporal lobe, the right temporal lobe, the central occipital lobe, the right parietal lobe, the central parietal lobe, and the left parietal lobe And increasing the ASEF (spectral edge frequency 50% of the alpha spectrum band) in the middle prefrontal cortex.
delete delete A health functional food for enhancing brain comfort comprising the dandelion mixed extract composition according to claim 1.
The composition according to claim 4, further comprising at least one compound selected from the group consisting of vitamins, minerals, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, And a carbonating agent used in a carbonated drink. &Lt; RTI ID = 0.0 &gt; 21. &lt; / RTI &gt;
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