CN113811320A

CN113811320A - Plant composition, preparation method and application thereof

Info

Publication number: CN113811320A
Application number: CN202080030908.0A
Authority: CN
Inventors: 董银卯; 孟宏; 秦春莉; 查沛娜
Original assignee: Taihe Kangmei Beijing Research Institute of Traditional Chinese Medicine Co Ltd
Current assignee: Taihe Kangmei Beijing Research Institute of Traditional Chinese Medicine Co Ltd
Priority date: 2019-05-31
Filing date: 2020-05-27
Publication date: 2021-12-17
Also published as: WO2020238983A1

Abstract

A plant composition, its preparation method and its application in sleep-promoting product are disclosed. The plant composition comprises the following components in parts by weight: 40-55 parts of spina date seed, 30-45 parts of rose and 5-15 parts of cinnamon. The composition adopts components which are homologous in medicine and food, safe and easily available as composition raw materials, has a formula for synergism, not only solves the multi-dimensional sleep problem remarkably, but also has the functions of improving skin microcirculation and skin temperature, namely improving skin metabolism capability, improving skin color uniformity, whitening and lightening spots, improving skin stasis state and accelerating skin pigment metabolism, and has a wide application prospect.

Description

Plant composition, preparation method and application thereof

Technical Field

The invention relates to a plant composition, in particular to a plant composition with sleep-aiding and beauty-maintaining effects, application of the plant composition in preparation of products with sleep-aiding and beauty-maintaining effects and a preparation process.

Background

The sleep occupies about 1/3 hours in the whole life cycle of human, has close relationship with the individual psychological and physiological health, can eliminate the physical and psychological fatigue of the individual and can effectively regulate the metabolism of the individual. The increasing pace of life and the increasing social competitive pressure in the modern times lead to more and more heavy psychological burdens of individuals, disordered work and rest time and endocrine dyscrasia, thereby causing sleep disorders with different degrees. Poor sleep quality not only can cause bad effects of mental fatigue, tiredness in daytime, and the like of patients, but also can cause poor circulation of qi and blood of the skin of people, thereby affecting the health and beauty of people. How to safely and effectively improve the sleep quality and improve the health and beauty of human skin is also an urgent problem to be solved in modern research.

At present, main components of common products for solving insomnia in the market comprise melatonin, vitamin B6 and the like, and although researches prove that the melatonin has a good treatment effect on sleep disorders caused by jet lag, sleep delay syndrome and the like, reports indicate that the mechanism of normal synthesis and melatonin secretion of an organism is directly inhibited by long-term overdose of the melatonin, so that the sleep function is disordered. How to safely and effectively solve insomnia and improve sleep quality still remain problems to be solved urgently.

Patent CN102188610B discloses a Chinese medicinal composition with sleep improving effect and its preparation method, wherein the composition is prepared by mixing Ganoderma, arillus longan, semen Ziziphi Spinosae, semen Platycladi, caulis Polygoni Multiflori, rhizoma Polygonati, fructus Jujubae and adjuvants at a certain proportion, and making into oral dosage form. However, the invention uses more raw materials, has complex components and higher cost.

Disclosure of Invention

Insomnia is caused by disharmony between the heart and spleen, which is considered by traditional Chinese medicine, and is caused by disharmony between the heart and spleen, and is mediated by the heart and spleen. Heart stores spirit, liver stores spirit, spleen distributes semen. The novel concept of comprehensively helping sleep and beautifying is considered on the principle of synchronously regulating the heart and the spleen, nourishing blood and moistening dryness and soothing the liver and regulating blood, the strong requirements of people on health, safety and no side effect of oral products are met, and the novel concept has practical significance and wide market prospect.

Aiming at the defects of the prior art, the invention provides a plant composition which is homologous in medicine and food and easy to obtain raw materials, and the plant composition has the advantages that all components are synergistic, can improve sleep, and has good cosmetic effects of improving skin microcirculation, whitening skin, homogenizing skin color and the like;

the second object of the present invention is to provide an extract comprising the plant composition and a method for preparing the same;

the third purpose of the invention is to provide the application of the plant composition or the plant extract in oral products, including but not limited to substitutional tea, powder, paste, oral liquid, granules, capsules, pills, tablets or powder and the like;

the fourth object of the present invention is to provide a powder comprising the plant composition or plant extract and a method for preparing the same;

the invention can solve the sleep problem from multiple dimensions, the sleep effect can be improved for a long time and effectively, and the invention has good beauty effect, especially has obvious effects on the aspects of improving the blood perfusion amount of the skin, increasing the skin temperature and the like.

In order to realize the purpose of the invention, the invention adopts the following technical scheme:

the invention provides a plant composition in a first aspect, which comprises the following components in parts by weight: 40-55 parts of spina date seed, 30-45 parts of rose and 5-15 parts of cinnamon.

According to the invention, the parts by weight of the wild jujube seeds in the plant composition are 40-55 parts, such as 40 parts, 41 parts, 42 parts, 43 parts, 44 parts, 45 parts, 46 parts, 47 parts, 48 parts, 49 parts, 50 parts, 51 parts, 52 parts, 53 parts, 54 parts, 55 parts, and the values between the above, preferably 45 parts.

Furthermore, the fried spina date seed has better effect and can be prepared by the following or similar processes: 1. sieving semen Ziziphi Spinosae with 14 mesh sieve; 2. crushing the screened spina date seeds by a flattening machine; 3. parching crushed semen Ziziphi Spinosae at 260 deg.C for 12-15 min; taking out of the pot at 4.115-120 ℃.

According to the invention, the rose is present in the plant composition in an amount of 30 to 45 parts by weight, such as 30 parts, 31 parts, 32 parts, 33 parts, 34 parts, 35 parts, 36 parts, 37 parts, 38 parts, 39 parts, 40 parts, 41 parts, 42 parts, 43 parts, 44 parts, 45 parts, and points between these values, preferably 40 parts.

According to the invention, the cinnamon is present in the plant composition in an amount of 5 to 15 parts by weight, such as 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, and dot values between these, preferably 15 parts.

The invention provides a plant composition, which comprises the following components in parts by weight: 20-50 parts of lily, 10-40 parts of fried spina date seed, 10-30 parts of rose and 1-20 parts of cinnamon.

According to the present invention, the lily is 20 to 50 parts by weight of the plant composition of the second aspect, for example, 20 parts, 21 parts, 22 parts, 23 parts, 24 parts, 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts, 31 parts, 32 parts, 33 parts, 34 parts, 35 parts, 36 parts, 37 parts, 38 parts, 39 parts, 40 parts, 41 parts, 42 parts, 43 parts, 44 parts, 45 parts, 46 parts, 47 parts, 48 parts, 49 parts or 50 parts, preferably the lily is 30 to 40 parts by weight of the plant composition, and a specific point value therebetween is more preferably 40 parts.

According to the invention, the weight part of the wild jujube seed in the plant composition of the second aspect is 10-40 parts, for example, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, 23 parts, 24 parts, 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts, 31 parts, 32 parts, 33 parts, 34 parts, 35 parts, 36 parts, 37 parts, 38 parts, 39 parts or 40 parts, preferably the weight part of the wild jujube seed in the plant composition is 15-35 parts, and the specific points between the above values are more preferably 35 parts.

According to the invention, the parts by weight of the roses in the plant composition of the second aspect are 10-30 parts, for example 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, 23 parts, 24 parts, 25 parts, 26 parts, 27 parts, 28 parts, 29 parts or 30 parts, and specific points between the above values, preferably the parts by weight of roses in the plant composition are 15-25 parts, more preferably 15 parts.

According to the invention, the cinnamon is present in the plant composition of the second aspect in an amount of 1 to 20 parts by weight, for example 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 parts by weight, preferably 5 to 15 parts by weight of cinnamon is present in the plant composition, and more preferably 10 parts by weight.

The composition and the proportion of the components in the composition are important for the efficacy of the composition, and the inventor finally determines that the combination of the lily, the spina date seed, the rose and the cinnamon has a synergistic effect through repeated tests, so that the efficacy of the composition can be greatly improved, and particularly, the fried spina date seed has a better effect.

In a third aspect, the present invention provides a plant extract prepared from the plant composition of the first or second aspect of the present invention.

In a fourth aspect of the present invention, there is provided a method for preparing the plant extract, the method comprising the steps of:

(1) weighing the raw materials according to the dosage;

(2) according to the material-liquid ratio of 1: 8-1: 15 in water for 20-40 minutes;

(3) adding 0.5-1% cellulase and 0.5-1% amylase, extracting at 45-50 deg.C, and heating to 75-80 deg.C for extraction;

(4) inactivating enzyme to obtain extractive solution.

According to the invention, the feed-liquid ratio in the step (2) is 1: 8-1: 15, for example, may be 1: 8. 1: 9. 1: 10. 1: 11. 1: 12. 1: 13. 1: 14 or 1: 15, and the point values between these values, preferably 1: 10.

according to the invention, the cellulase in step (3) is used in an amount of 0.5 to 1%, and may be, for example, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, 0.85%, 0.9%, 0.95%, or 1%, and specific values therebetween, preferably 1%.

According to the invention, the amylase is used in step (3) in an amount of 0.5-1%, for example 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, 0.85%, 0.9%, 0.95% or 1%, and values between these values, preferably 1%.

According to the invention, 0.5-1% pectinase may also be added in step (3) together with cellulase and amylase, and the amount of pectinase may be, for example, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, 0.85%, 0.9%, 0.95%, or 1%, and specific values between the above, preferably 1%.

The selection of the amount and type of enzyme has a great influence on the extraction effect, and the inventors surprisingly found that the combination and amount of the above enzymes provides the best extraction effect.

According to the invention, the extraction conditions in step (3) are: extracting in water bath at 45-50 deg.C for 1-3 hr, preferably 2 hr; then heating to 75-80 ℃ and extracting in water bath for 2-3h, preferably 2 h; the selection of the extraction temperature and the extraction time is crucial to the extraction efficiency, the property of an extract, the extraction efficiency and the like can be influenced by improper extraction temperature and extraction time, and the extraction time and the extraction temperature under the experimental conditions are optimal.

In some preferred embodiments, step (3) of the present invention controls the pH to be 4.5 to 7.5, and may be, for example, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.1, 7.2, 7.3, 7.4 or 7.5, and specific values therebetween, preferably 4.5 to 5.5.

In some embodiments, step (3) uses citric acid to control the pH.

According to the invention, enzyme deactivation is carried out for 25-40min at 90-100 ℃ in the step (4).

The selection of parameters and steps of the preparation process has great influence on the extraction rate and the extraction effect of the extract, and the inventor finally obtains the preparation process of the third aspect of the invention through repeated experiments, and the preparation process has important significance in improving the extraction efficiency and saving the production cost.

In a fifth aspect, the invention provides the use of the plant composition or the plant extract. The plant composition or plant extract can be used for products with various formulations, such as substitutional tea, powder, paste, oral liquid, granules, capsules, pills, tablets or powder and the like.

According to some specific examples of the invention, the composition of the invention may be used in tea substitutes prepared by the steps of:

(1) respectively crushing the raw materials of the composition;

(2) sieving by using a 4-20-mesh sieve, and taking the sieved part;

(3) sieving the sieved part with a 40-65-mesh sieve, and taking the sieved part;

(4) mixing at a certain proportion, and packaging.

According to some embodiments of the invention, the substitutional tea is prepared by:

(1) respectively crushing the raw materials of the composition;

(2) sieving by using a 4-20-mesh sieve, and taking the sieved part;

(4) the components are uniformly mixed according to the proportion and then are subpackaged by a packaging machine, and a bag is filled with 2.5-5 g of the mixture of the composition.

The recommended use method of the substitutional tea provided by the invention comprises the following steps: infusing with hot water.

According to a sixth aspect of the present invention, there is provided a powder formulation, which, according to some preferred embodiments of the present invention, is prepared by:

(1) weighing the raw materials according to the dosage;

(2) the raw materials in the step (1) are mixed according to a material-liquid ratio of 1: 8-1: 15 in water for 20-40 minutes;

(3) adding 0.5-1% cellulase and 0.5-1% amylase, extracting at 45-50 deg.C, heating to 75-80 deg.C, and extracting;

(4) inactivating enzyme to obtain extractive solution;

(1) coarsely filtering the extract with a 400-mesh gauze of 180 meshes, and cooling to 30-45 ℃ to obtain coarse filtrate;

(2) fine filtering the crude filtrate with H70 (pore size of 0.45 μm) paper board to obtain fine filtrate;

(3) adding 10-35% dextrin, dissolving, and concentrating to solid content of 15-35% to obtain concentrated solution;

(4) drying the concentrated solution to obtain powder.

according to the invention, the cellulase in step (3) is used in an amount of 0.5 to 1%, and may be, for example, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, 0.85%, 0.9%, 0.95%, or 1%, and specific values therebetween, more preferably 1%.

According to the invention, the amylase is used in step (3) in an amount of 0.5-1%, for example 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, 0.85%, 0.9%, 0.95% or 1%, and values between the above values, more preferably 1%.

According to the invention, 0.5-1% pectinase may also be added in step (3), which may be, for example, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, 0.85%, 0.9%, 0.95%, or 1%, as well as values specifically between the above values, more preferably 1%.

According to the invention, the extraction conditions in step (3) are: extracting in water bath at 45-50 deg.C for 1-3 hr, preferably 2 hr; then extracting with 75-80 deg.C water bath for 2-3 hr, preferably 2 hr.

In some preferred embodiments, step (3) of the present invention controls the pH to be 4.5 to 7.5, and may be, for example, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.1, 7.2, 7.3, 7.4 or 7.5, and specific values therebetween, preferably 4.5 to 5.5, more preferably 4.8.

In some embodiments, step (3) uses citric acid to control the pH.

According to the invention, the dextrin in step (7) is preferably a resistant dextrin and/or a maltodextrin.

According to the invention, freeze drying is adopted in the step (8), and the freeze drying conditions are as follows: the temperature is-76 ℃, the pressure is 60-70mTor, and the time is 24 h.

The preparation method of the plant extract, the powder, the substituted tea and the like is not limited to the preparation method, and other conventional methods in the field can achieve the effects, and the preparation method is recommended by the inventor.

The invention has the beneficial effects that:

(1) the raw materials selected by the invention are all medicinal and edible raw materials, and are wide in source, safe and easy to obtain.

(2) The preparation method has the advantages of simple preparation process and high extraction rate, and is suitable for industrial production.

(3) The invention has obvious advantages in the aspects of sleep aiding and beauty treatment, not only solves the problem of multidimensional sleep remarkably, but also has the functions of improving skin microcirculation and skin temperature, namely improving skin metabolism capability; meanwhile, the skin whitening cream has the effects of whitening, homogenizing skin color, lightening spots and relieving sensitive symptoms.

The invention adopts medicinal and edible raw materials, researches and develops a characteristic plant efficacy formula according to the traditional Chinese medicine formula principle, has unique advantages in the aspects of sleep aiding and beauty treatment, has wide and easily-obtained raw material sources, simple preparation process and low preparation cost, and meets the strong requirements of people on health and safety of oral products.

Drawings

FIG. 1 is a graph showing the results of a blood perfusion test;

FIG. 2 is a graph showing the results of VISIA-CR standard light 2 test;

FIG. 3 is a VISIA-CR UV light test result chart;

FIG. 4 is a VISIA-CR cross-polarized light test result chart.

Detailed Description

In order to make the technical solution of the present invention better understood by those skilled in the art, the present invention will be described in detail below with reference to the embodiments and the accompanying drawings. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting of the invention. The examples, which are not specifically shown for the specific methods, are all routine in the art or according to the product specifications. The reagents or apparatus used are conventional products commercially available from normal sources, not indicated by the manufacturer.

Table 1 raw materials supplier list

Components	Source of raw materials
Lily bulb	Beijing Qiancao Chinese herbal pieces Limited
Parched semen Ziziphi Spinosae	Beijing Qiancao Chinese herbal pieces Limited
Rose	Beijing Qiancao Chinese herbal pieces Limited
Cortex Cinnamomi	Beijing Qiancao Chinese herbal pieces Limited
Raw wild jujube kernel	Beijing Qiancao Chinese herbal pieces Limited
Resistant dextrins	Baolingbao Biological Co., Ltd.
Maltodextrin	Baolingbao Biological Co., Ltd.
Cellulase enzymes	Beijing Yinuo Biotech Co Ltd
Pectinase	Beijing Yinuo Biotech Co Ltd
Amylase	Beijing Yinuo Biotech Co Ltd
Wild jujube seed	Beijing Qiancao Chinese herbal pieces Limited

Example 1 preparation of substitutional tea

(1) Pulverizing Bulbus Lilii, parched semen Ziziphi Spinosae, flos Rosae Rugosae, and cortex Cinnamomi respectively;

(2) sieving with a 10-mesh sieve, and taking the sieved part;

(3) sieving the sieved part with a 50-mesh sieve, and taking the sieved part;

(4) uniformly mixing 40 parts by weight of lily, 35 parts by weight of fried spina date seed, 15 parts by weight of rose and 10 parts by weight of cinnamon, and subpackaging by using a packaging machine, wherein 2.5g of the mixture of the composition is taken as one bag.

Example 2 tea substitute preparation

(2) sieving with 4 mesh sieve, and collecting the sieved part;

(3) sieving the sieved part with a 40-mesh sieve, and taking the sieved part;

(4) uniformly mixing 20 parts by weight of lily, 40 parts by weight of fried spina date seed, 10 parts by weight of rose and 20 parts by weight of cinnamon, and subpackaging by using a packaging machine, wherein 3.5g of the mixture of the composition is used as one bag.

Example 3 substitutional tea preparation

(2) sieving with a 20-mesh sieve, and taking the sieved part;

(3) sieving the sieved part with a 65-mesh sieve, and taking the sieved part;

(4) mixing Bulbus Lilii 50 weight parts, parched semen Ziziphi Spinosae 10 weight parts, flos Rosae Rugosae 30 weight parts, and cortex Cinnamomi 1 weight parts, packaging with packaging machine, and taking 5g mixture of the composition as a bag.

Example 4 tea substitute preparation

(1) Respectively pulverizing Bulbus Lilii, semen Ziziphi Spinosae, flos Rosae Rugosae, and cortex Cinnamomi;

(2) sieving with a 20-mesh sieve, and taking the sieved part;

(3) sieving the sieved part with a 50-mesh sieve, and taking the sieved part;

(4) uniformly mixing 40 parts by weight of lily, 35 parts by weight of spina date seed, 15 parts by weight of rose and 10 parts by weight of cinnamon, and subpackaging by using a packaging machine, wherein a bag is filled with a mixture of 2.5g of the composition.

Example 5 tea substitute preparation

(1) Respectively pulverizing parched semen Ziziphi Spinosae, flos Rosae Rugosae, and cortex Cinnamomi;

(2) sieving with a 10-mesh sieve, and taking the sieved part;

(3) sieving the sieved part with a 50-mesh sieve, and taking the sieved part;

(4) uniformly mixing 45 parts of fried spina date seeds, 40 parts of roses and 15 parts of cinnamon, and subpackaging by using a packaging machine, wherein 2.5g of the mixture of the composition is used as one bag.

Comparative examples 1 to 5

TABLE 2

Evaluation of efficacy

(1) Volunteer recruitment and sleep statistics

96 subjects were recruited, and the sleep of the subjects was recorded by means of a sleep diary, and after 1 week of recording, the subjects were randomly divided into 8 groups, and the groups of the substitutional tea of example 1, example 4, example 5 and comparative examples 1 to 5 were drunk 1 bag per day for 1 week continuously. The sleep diary format is as follows.

TABLE 3

The mean improvement in sleep by the sleep diary record for each group of subjects after 1 week of drinking compared to before drinking is shown in the following table:

TABLE 4

Note: the sleep efficiency is 100% of the actual sleep time/time in bed, and the actual sleep time in the sleep diary is the time in bed minus the time required to fall asleep. Before the volunteers are grouped, strict sleep quality investigation and screening are carried out, and strict sleep evaluation method training is carried out after the volunteers are grouped.

As can be seen from the above table, after the sample of the embodiment 1 of the invention is tried, the sleeping time and the sleeping efficiency of a testee are obviously improved, the sleeping-aid effect of the composition is obviously higher than that of a single medicine or other combinations, and the components have a synergistic effect. After the test sample of the embodiment 5 of the invention is tried, the sleep efficiency of the test subject is obviously improved, the sleep aiding effect of the composition of the invention is obviously higher than that of a single medicine or other combinations, and the components have a synergistic interaction effect. The embodiment 1 and the embodiment 4 have obvious sleep improvement effect, and compared with the raw wild jujube kernel, the fried wild jujube kernel and other components are combined to have more obvious sleep improvement effect.

The above results show that the composition of the present invention has a significant effect on improving sleep.

The inventor further researches the preparation process of the combined extract and explores the influence of the conditions such as extraction temperature, extraction time, compound enzymolysis and the like on the extraction rate of the active ingredients of the extract. Uniformly mixing 40 parts of lily, 35 parts of fried spina date seed, 15 parts of rose and 10 parts of cinnamon, and mixing the raw materials according to a material-liquid ratio of 1: 10 in water for 30 minutes, and the extracts were prepared according to the experimental methods shown in table 5, respectively, with the pH being controlled to 4.8 during the enzymatic hydrolysis using citric acid.

Firstly, the kind of complex enzyme is considered, the higher the solid content value is, the higher the representative extraction rate is, the higher the solid content value is, the solid content value of the enzymatic hydrolysis of different kinds of complex enzyme under the fixed condition is recorded respectively, and the result is shown in table 5:

TABLE 5

(Table 5 Experimental conditions: after 3 hours in 50 ℃ water bath, pH was controlled to 4.8)

As shown in Table 5, the solid content can be significantly improved by compounding cellulase, pectinase and amylase by adopting the cellulase and the amylase. The combination of cellulase and amylase may be preferred for cost reasons.

The inventor further inspects the temperature and time of the complex enzyme enzymolysis, takes the solid content value as an index, the higher the solid content value is, the higher the extraction rate is, and respectively records the solid content values of 1h, 2h, 3h, 4h and 5h extracted under different enzymolysis conditions, and the results are shown in table 6:

TABLE 6

As can be seen from the experimental results shown in Table 6, the solid content of the obtained extract is higher and the extraction rate is higher by performing enzymolysis in a 50 ℃ water bath and then heating the water bath to 80 ℃.

The inventor further considers the influence of different compound enzyme dosages and proportions on the solid content value of the extracting solution, when the cellulase dosage is 0.5-1% and the amylase dosage is 0.5-1%, the solid content value is above 5.8, the effect is optimal when the cellulase dosage and the amylase dosage are 1%, and the solid content value is as high as 7.2 after 4h of extraction (wherein, the raw materials used in examples 15 and 16 and the preparation method thereof are that 45 parts by weight of fried spina date seeds, 40 parts by weight of rose and 15 parts by weight of cinnamon are uniformly mixed, soaked in water for 30 minutes according to the proportion of 1: 10 of the material-to-liquid ratio, and the pH is controlled to be 4.8 during enzymolysis, and the influence of different compound enzyme dosages and proportions on the solid content value of the extracting solution is considered), and the results are as follows:

TABLE 7

(Table 7 Experimental conditions: after 50 ℃ water bath for 2h, temperature rise in 80 ℃ water bath for 2h, pH control 4.8)

The inventor further prepares a powder product by taking the plant composition as a raw material and carries out clinical observation and human body efficacy evaluation experiments.

Preparation of powder products:

EXAMPLE 17 powder preparation

(1) Weighing 40 parts of lily, 35 parts of fried spina date seed, 15 parts of rose and 10 parts of cinnamon;

(2) according to the material-liquid ratio of 1: 10 in water for 30 minutes;

(3) adjusting the pH value to 4.8 by using citric acid, adding 1% of cellulase and 1% of amylase, carrying out water bath at 50 ℃ for 2h, and heating to 80 ℃ for 2 h;

(4) heating to 90 deg.C to inactivate enzyme for 30min to obtain extract;

(5) coarse filtering the extract with 180 mesh gauze, cooling to 35 deg.C to obtain coarse filtrate;

(6) fine filtering the crude filtrate with H70 (pore size of 0.45 μm) paper board to obtain fine filtrate;

(7) adding 30% maltodextrin, dissolving, and concentrating by rotary evaporation until the solid content is 20% to obtain concentrated solution;

(8) drying the concentrated solution: the temperature is-76 ℃, the pressure is 60-70mTor, and the time is 24 hours, thus obtaining the powder.

EXAMPLE 18 powder preparation

(1) Weighing 35 parts of lily, 25 parts of fried spina date seed, 18 parts of rose and 5 parts of cinnamon;

(2) according to the material-liquid ratio of 1: 15 in water for 20 minutes;

(3) adjusting the pH value to 4.5 by using citric acid, adding 0.75% of cellulase and 1% of amylase, carrying out water bath at 45 ℃ for 2h, and heating to 80 ℃ for 2 h;

(4) heating to 90 deg.C to inactivate enzyme for 30min to obtain extractive solution;

(5) coarse filtering the extractive solution with 400 mesh gauze, cooling to 45 deg.C to obtain coarse filtrate;

(7) adding 10% resistant dextrin, dissolving, and concentrating until the solid content is 35% to obtain a concentrated solution;

EXAMPLE 19 powder preparation

(1) Weighing 30 parts of lily, 25 parts of fried spina date seed, 20 parts of rose and 10 parts of cinnamon;

(2) according to the material-liquid ratio of 1: 10 in water for 30 minutes;

(3) adjusting the pH value to 5.5 by using citric acid, adding 0.75% of cellulase and 1% of amylase, carrying out water bath at 50 ℃ for 2h, and heating to 80 ℃ for 2 h;

(7) adding 35% maltodextrin, dissolving, and concentrating until the solid content is 15% to obtain concentrated solution;

EXAMPLE 20 powder preparation

(1) Weighing 35 parts of lily, 35 parts of fried spina date seed, 25 parts of rose and 15 parts of cinnamon;

(2) according to the material-liquid ratio of 1: 8 in water for 40 minutes;

(3) adjusting the pH value to 5.0 by using citric acid, adding 0.5% of cellulase and 1% of amylase into the mixture, performing water bath at 50 ℃ for 1h, and heating the mixture to 75 ℃ for water bath for 2 h;

(4) heating to 100 deg.C to inactivate enzyme for 25min to obtain extractive solution;

(7) adding 10% maltodextrin, dissolving, and concentrating until the solid content is 35% to obtain concentrated solution;

EXAMPLE 21 powder preparation

(1) Weighing 30 parts of lily, 20 parts of fried spina date seed, 30 parts of rose and 20 parts of cinnamon;

(2) according to the material-liquid ratio of 1: 15 in water for 20 minutes;

(3) adjusting the pH value to 6.0 by using citric acid, adding 1% of cellulase and 0.5% of amylase into the mixture, performing water bath at 45 ℃ for 2 hours, and heating the mixture to 75 ℃ for 3 hours;

(4) heating to 100 deg.C to inactivate enzyme for 40min to obtain extractive solution;

(5) coarsely filtering the extractive solution with 200 mesh gauze, and cooling to 45 deg.C to obtain coarse filtrate;

(7) adding 18% maltodextrin, dissolving, and concentrating until the solid content is 25% to obtain concentrated solution;

EXAMPLE 22 powder preparation

(1) Weighing 45 parts of fried spina date seeds, 40 parts of roses and 15 parts of cinnamon;

(2) according to the material-liquid ratio of 1: 10 in water for 30 minutes;

(3) adjusting the pH value to 4.8 by using citric acid, adding 1% of cellulase and 0.5% of amylase into the mixture, performing water bath at 50 ℃ for 2 hours, and heating the mixture to 80 ℃ for water bath for 2 hours;

(5) coarse filtering the extractive solution with 180 mesh gauze, cooling to 35 deg.C to obtain coarse filtrate;

(7) adding 30% maltodextrin, dissolving, and concentrating to solid content of 20% to obtain concentrated solution;

EXAMPLE 23 powder preparation

(1) Weighing 40 parts of spina date seed, 40 parts of rose and 15 parts of cinnamon;

(2) according to the material-liquid ratio of 1: 10 in water for 30 minutes;

(3) adjusting the pH value to 4.8 by using citric acid, adding 1% of cellulase and 1% of amylase into the mixture, performing water bath at 50 ℃ for 2 hours, and heating the mixture to 75 ℃ for water bath for 2 hours;

The inventor further adopts evaluation methods such as Pittsburgh sleep quality index and the like to observe 36 other volunteers drinking the powder for 4 weeks for a long time, and verifies the effects of the powder on improving sleep conditions, skin blood perfusion and skin temperature in multiple angles.

Clinical observation and human efficacy evaluation test 1:

volunteer recruitment: 36 cases. The age is 20-60 years, the average age (31.97 +/-12.02) years, 7 male cases and 29 female cases. Insomnia is present in 7 cases for years; insomnia is greater than 6 months, and 17 cases are less than 1 year; the insomnia is more than 4 weeks, and 5 cases are found in less than 3-6 months; insomnia was less than 7 cases in 4 weeks.

Volunteers drunk the powder prepared in example 17 of the present invention 1 bag a day for 1 week. Subjects filled out Pittsburgh Sleep Quality Index (PSQI) scale at week 0, after week 1, and after week 4, respectively.

The evaluation method comprises the following steps:

sleep disorder scoring

The sleep of the subject was assessed using the Pittsburgh Sleep Quality Index (PSQI) scale. Pittsburgh Sleep Quality Index (PSQI), which was proposed by Buysse et al in 1989, has good intrinsic agreement (Cronbach's α is 0.83) and re-confidence (r is 0.85), and can effectively assess sleep quality. The PSQI is composed of 7 dimensions, namely a component A (sleep quality), a component B (sleep time), a component C (sleep time), a component D (sleep efficiency), a component E (sleep disorder), a component F (hypnotic), and a component G (daytime function), wherein each dimension is 0-3 points, the total point range is 0-21 points, and the higher the point is, the worse the sleep quality is. Wherein 5-6 points are the critical state of insomnia, more than 7 points are taken as the standard of sleep disorder, 7-11 points are mild, 12-16 points are moderate, and 17-21 points are severe.

The Self-Rating Scale (Self-Rating Anxiety Scale SAS) scores, and the Self-Rating Scale (Self-Rating Anxiety Scale SAS) scores adopt 4 grades of scores, and mainly evaluate the frequency of symptom appearance. According to the results of the Chinese norm, the SAS standard score has a cut-off value of 50, wherein 50-59 scores are mild anxiety, 60-69 scores are moderate anxiety, and more than 70 scores are severe anxiety.

Scoring by a depression self-scoring Scale (Se1f-Rating depression scope, SDS), scoring by a depression self-scoring Scale (Se1f-Rating depression Scale, SDS), wherein according to the result of Chinese orthodox, the cut-off value of the SDS standard score is 53 points, wherein 53-62 points are mild depression, 63-72 points are moderate depression, and more than 72 points are severe depression.

Exclusion criteria

Samples with the following cases should not be listed as panelists, to the exclusion:

(1) patients diagnosed with psychiatric disorders such as major anxiety, depression, bipolar disorder, schizophrenia, etc. in the past 3 months;

(2) patients with drug abuse;

(3) there are other sleep-related disorders, such as: somnolence, abnormal sleep;

(4) mental disorder, central nervous system disease, brain tumor, dementia or mild cognitive impairment;

(5) pregnant and lactating women.

Selection criteria are as follows:

(1) for people suffering from sleep disturbance, the Pittsburgh sleep quality index meets the condition that PSQI is more than or equal to 7;

(2) meanwhile, the depression self-rating scale score SDS <72 and the anxiety self-rating scale score SAS <70 are selected.

Drinking method

Infusing with hot water, and drinking one bag 1-2 hours before sleeping every day. And evaluated again for sleep disorders after 1 week and 4 weeks, respectively.

Evaluation criteria of sleep curative effect

Firstly, curing: the PSQI score is not less than 7 and is not more than 4;

secondly, effect is displayed: the insomnia grade is reduced if the insomnia is not cured, such as the original severe/moderate insomnia is changed into mild insomnia;

③ improving: the insomnia is not cured, the grade of the insomnia is not changed, but the score is reduced by more than or equal to 2 points;

fourthly, invalidation: the score was decreased by 1 point or was unchanged or increased.

The fifth step is effective: cure, obvious effect and improvement.

Statistical method

The SPSS 23.0 software is adopted to carry out paired sample t test on the scores and the total scores of all components of Pittsburgh sleep quality scales (PSQI), skin data and other related indexes of 36 subjects in the test after drinking the powder for 0, 1 and 4 weeks.

Evaluation results were as follows:

and (3) sleep statistics results:

TABLE 8

Note: 1. statistical significance (P <0.05) compared to before use (week 0); indicates statistical significance (P < 0.01).

After 1 week and 4 weeks of drinking, the mean value and standard deviation of the component F (hypnotic) were 0. The sleep-aiding powder is a common food and a non-medicine.

From the results, it was found that the PSQI of 36 subjects 1 week later had statistical significance for component a (sleep quality), component B (time to fall asleep), component C (sleep time), component E (sleep disturbance), component G (daytime function), and the total score compared to before drinking (week 0); after 4 weeks, component A (sleep quality), component B (sleep onset time), component C (sleep time), component G (daytime function), and the total score were statistically significant compared to those before drinking (week 0). The invention can effectively improve the sleeping quality of the testee, shorten the time of falling asleep and prolong the sleeping time, and has obvious effects on the aspects of drowsiness, insufficient energy and the like of the testee in the daytime.

The treatment results are as follows:

TABLE 9

After 4 weeks of drinking, 12 cases are cured, 17 cases are obviously effective, 3 cases are improved, the total effective rate is the cure rate plus the obvious and good conversion rates, and the effective rate is 89%.

Subjective evaluation results:

the subjects had subjective evaluation of the presence or absence of changes in the overall sleep quality after drinking the tea, and the results are shown in the following table:

watch 10

	Improved but not obvious	Has great improvement	Without improvement	Others
Number of people in 1 week	28	6	1	1
1 week (%)	78％	17％	3％	3％
4 weeks	22	13	1	0
4 weeks (%)	61％	36％	3％	0％

After 1 week of drinking, 94% (34) of 36 subjects showed an improvement in overall sleep quality; after 4 weeks of drinking, 97% (35) of 36 subjects showed an improvement in overall sleep quality.

Skin improvement criteria

Subjects were tested for perfusion volume and skin temperature at week 0, after week 1, and after week 4. The blood perfusion and skin temperature data were statistically significant (P <0.05) after 4 weeks compared to before drinking (week 0).

Skin test results:

TABLE 11

Note: statistical significance (P <0.05) compared to before use (week 0); indicates statistical significance (P < 0.01).

As can be seen from the table above, the two indexes of the blood perfusion amount and the skin temperature of the subject after 4 weeks have statistical significance compared with those before drinking (week 0), which shows that the invention not only can effectively solve various sleep disorders and improve the sleep quality, but also can improve the facial qi and blood circulation of the subject. Fig. 1 shows the blood perfusion amount picture before and after the test of part of volunteers, and the results in fig. 1 show that the powder of the invention has the effects of improving skin microcirculation and promoting skin metabolism.

The inventor adopts a scientific skin beauty detection instrument VISIA-CR to observe the cheek part of a subject and evaluates the skin improvement effect of the subject before and after using the product.

FIG. 2 is a VISIA-CR image (Standard light 2 Flat light) showing that the skin of the subject is more white and the skin color is uniform after the powder of the present invention is tried, which shows that the present invention has the effects of improving the uniformity and whitening of the skin.

FIG. 3 is a photograph of a VISIA-CR image (UV spots) showing that the powder of the present invention is used by a subject to reduce the UV spots, indicating that the powder of the present invention has a spot-lightening effect.

Fig. 4 shows the results of VISIA-CR imaging photographs (cross-polarized red), and it can be seen that after the powder of the present invention is tried on a subject, the red area becomes lighter in color and smaller in area, which shows that the present invention has the effects of relieving the state of skin stasis and accelerating the skin pigment metabolism.

Clinical observation and human body efficacy evaluation test 2

Volunteer recruitment: and (12) cases. The age is 20-60 years, and 3 cases of insomnia are existed for years; insomnia is greater than 6 months, and 3 cases are less than 1 year; the insomnia is more than 4 weeks, and 3 cases are found in less than 3-6 months; insomnia was less than 3 cases of 4 weeks.

Exclusion criteria

(2) patients with drug abuse;

(5) pregnant and lactating women.

Selection criteria are as follows:

(1) for people suffering from sleep disturbance, the Pittsburgh sleep quality index meets the condition that PSQI is more than or equal to 5;

Volunteers drunk the powder prepared in example 22 of the present invention 1 bag a day for 1 week. The subjects feed back the sleep data on the bracelet every day, fill in the sleep diary and score the sleep before and after the night (score range is 2-10, the higher the score is, the better the sleep quality), and respectively fill in the sleep condition questionnaire after 1 week and after 2 weeks after 0 week, 1 week and 2 weeks.

Drinking method

Infusing with hot water, and drinking one bag 1-2 hours before sleeping every day.

Testing tool

Glowing bracelet 4: the testee wears the bracelet about unusual wrist carpal bone 1 finger as required, transmits sleep data to cell-phone APP through the bluetooth, feeds back the sleep index (sleep score, sleep duration, deep sleep time, light sleep time, quick eye movement sleep time, deep sleep proportion, light sleep proportion, quick eye movement sleep proportion, deep sleep continuity, breathing quality etc.) every day.

Scoring the sleep diary:

1. how is it felt earlier today?

2. How do you sleep at yesterday and night?

Statistical method

SPSS 23.0 software is adopted to carry out paired sample t test on related indexes such as bracelet sleep data and sleep diary subjective scores of 12 experimental subjects after drinking the powder for 0, 1 and 2 weeks.

Evaluation results were as follows:

TABLE 12

As shown in the above table, the sleep deep sleep ratio of the subjects after 1 week, 2 weeks and 4 weeks is statistically significant (P <0.01) compared with the mean value at week 0, and the sleep score of the subjects after 1 week, 2 weeks and 4 weeks is statistically significant (P <0.05) compared with the mean value at week 0. The product has obvious effect on improving the integral sleep and deep sleep ratio of the testee.

Subjective scoring of sleep diaries

Watch 13

Note: statistical significance (P <0.05) compared to before use (week 0); indicates statistical significance (P <0.01)

As can be seen from the data in the table, the subject had an elevated self-rated sleep score, with statistical significance at week 1 compared to week 0 (P <0.05), and statistical significance at weeks 2, 3, and 4 compared to week 0 (P < 0.01).

TABLE 14

As can be seen from the data in the table, 8 of 12 subjects at week 2 felt increased sleep time, nighttime dreaminess or nightmare, and on the following day felt energetic and mood pleasant, accounting for 73%; 10 people feel that the sleep is fast and the sleepiness in the daytime is reduced, accounting for 91 percent.

Claims

The plant composition is characterized by comprising the following components in parts by weight: 40-55 parts of spina date seed, 30-45 parts of rose and 5-15 parts of cinnamon.
The plant composition is characterized by comprising the following components in parts by weight: 20-50 parts of lily, 10-40 parts of spina date seed, 10-30 parts of rose and 1-20 parts of cinnamon.
A plant extract prepared from the plant composition of claim 1 or 2 as a raw material.
A plant extract as claimed in claim 3, prepared by a process comprising the steps of:

(5) weighing the raw materials according to the dosage;

(6) the raw materials in the step (1) are mixed according to a material-liquid ratio of 1: 8-1: 15 in water for 20-40 minutes;

(7) adding 0.5-1% of cellulase and 0.5-1% of amylase, extracting at 45-50 ℃, and then heating to 75-80 ℃ for extraction;

(8) inactivating enzyme to obtain extract.
The method for preparing a plant extract according to claim 4, wherein 0.5% to 1% of pectinase is added together with cellulase and amylase in the step (3).
The method for preparing a plant extract as claimed in claim 4, wherein the extraction conditions in the step (3) are: extracting in water bath at 45-50 deg.C for 1-3 hr, heating to 75-80 deg.C, and extracting in water bath for 2-3 hr.
The method for preparing a plant extract according to claim 4, wherein in the step (3), the pH is controlled to 4.5 to 7.5; in the step (4), enzyme deactivation is carried out for 25-40min at 90-100 ℃ to obtain the extract.
Use of a plant composition according to any one of claims 1 or 2 or a plant extract according to any one of claims 3 to 7 in an oral product.
The use according to claim 8, wherein the oral product is substituted tea, powder, paste, oral liquid, granules, capsules, pills, tablets or powder.
A powder preparation obtained by filtering the plant extract according to any one of claims 3 to 7, mixing with dextrin, concentrating, and drying, preferably the dextrin is maltodextrin and/or resistant dextrin.