KR101982816B1 - Composition for removing hangover and improving liver function containing Saltwort - Google Patents

Abstract

Since a composition for relieving hangover and enhancing liver function according to the present invention contains an extract of saltwort, the composition is effective for the activity of alcoholytic enzymes to effectively control the alcohol concentration in blood, and has an excellent effect for protecting hepatocytes. Therefore, the composition can be commercialized into a health functional food comprising the same to be easily and simply used for hangover relief and liver function enhancement of modern people.

Description

Technical Field [0001] The present invention relates to a composition for eliminating hangover and liver function including green tea,

The present invention relates to a composition for ameliorating hangover and improving liver function comprising green tea.

An adequate amount of alcohol eliminates stress, appetite, and eliminates fatigue. However, excessive drinking and chronic drinking are adversely affecting your health.

The general alcohol metabolism is absorbed mainly from the gastrointestinal tract or small intestine when it is consumed by alcohol. It is decomposed by alcohol dehydrogenase (ADH) in gastrointestinal tract to minimize the absorption of alcohol through blood And the rest is transferred to the liver and metabolized. Approximately 90% of alcohol reached in the liver is oxidized to acetaldehyde by ADH and then acetic acid by acetaldehyde dehydrogenase (ALDH). The remaining 10% of the alcohol is metabolized to acetaldehyde by catalase. Acetaldehyde, which is the primary metabolite of the alcohol consumed in the process, induces nausea, vomiting, dizziness, thirst and lethargy. In addition, alcoholic fatty liver or alcoholic hepatitis occurs, and if you continue to drink alcohol in bad liver, cirrhosis may occur.

Recently, a variety of products have been introduced to help prevent fat accumulation, liver damage, and hangover due to increased drinking of modern people. However, studies on the composition of hangover detoxification using unique materials are scarce.

On the other hand, it is said to be called "Hamcho" in the oriental medicine, and "Tengtungmadi" which is used for medicinal purposes is known to contain Choline and Betain, dietary fibers and minerals. In the United States, The effects of hangover removal and liver function improvement are not known precisely.

Due to the frequent drinking of modern people, there is a high interest in a composition or a manufacturing method for removing hangover, and Patent No. 10-0853078 entitled " Method for producing herbal composition for hangover resolution ", Registered Patent No. 10-0969227 " A composition for preventing or eliminating a hangover, and a method for producing the same, " and a method for preparing the composition for preventing or eliminating a hangover, which are disclosed in Korean Patent Laid-Open Publication No. 10-2004-0056769 " And a method for producing the same, " but the technical characteristics of the present invention are different.

Accordingly, the inventors of the present application found that the extracts of Ranunculus Hamcho were excellent in the control of blood alcohol level and hepatocyte protection while studying a composition effective for hangover resolution and improvement of hepatic function. The present inventors have completed the present invention in which the hangover resolution and the liver protection function are further enhanced through additional experiments.

Patent No. 10-0853078 Registration No. 10-0969227 Patent Publication No. 10-2004-0056769 Patent Publication No. 10-2011-0033644 Japanese Patent Application Laid-Open No. 10-2017-0085451

In order to solve the above-mentioned problems, the present inventors intend to provide a composition for relieving hangover and improving liver function comprising a green tea extract.

The present invention also provides a health functional food comprising the composition for improving hangover and liver function.

To achieve the above object, the present invention provides a composition for relieving hangover and improving liver function comprising a green tea extract.

The present invention also provides a health functional food composition comprising a composition for eliminating hangover and liver function according to the present invention.

The composition for relieving hangover and improving liver function according to the present invention comprises a green tea extract as a main component and the green tea extract may be contained in an amount of 1 to 20% by weight based on the total weight of the composition for improving hangover and improving liver function, By weight to 15% by weight.

The compositions for improving hangover and improving liver function may further comprise at least one selected from the group consisting of Gugija extract, green tea extract, mung bean extract, citrus extract, licorice extract, astragalus extract, chrysanthemum extract, cinnamon extract, chrysanthemum extract, , Ginger extract, sine extract, ginger extract, but are not limited thereto.

The extracts of Gugija extract, Kugai extract, Red bean extract, Gangbyeol extract, Sein extract and Ginger extract are contained in an amount of 1 to 15 : 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 15: 1 to 10: 1 to 10: 1 to 10: To 10: 1 to 10 by weight, but not limited thereto.

The extracts of the present invention can be prepared by using conventional extraction methods for preparing medicinal plants and natural products. The extraction method is preferably one selected from the group consisting of hot water extraction, cold extraction, reflux cooling extraction, ultrasonic extraction and steam extraction, but is not limited thereto.

It is preferable to extract at a temperature of 50 to 120 ° C in hot water extraction. If the extraction is performed at a temperature lower than the above temperature, the active ingredient may not be extracted. If the extraction is performed at a temperature higher than the above temperature, the active ingredient may be destroyed .

The extracting time is preferably 2 to 10 hours. If the extracting time is shorter than the extracting time, an effective ingredient may not be extracted. If extracting more time than the extracting time, a large number of impurities may be extracted in addition to the effective ingredient to be.

In addition, the present invention can provide a health functional food composition including the composition for improving hangover and liver function, and the health functional food can be manufactured in various forms by a conventionally known method. For example, a liquid (beverage, pouch), a tablet, a pill, a hard capsule, a soft capsule, a granule, and the like.

The health functional food composition may be prepared by a conventional method known in the art and further includes additives commonly used in the production of health functional foods such as nutrients, vitamins, electrolytes, sweeteners, colorants, organic acids, can do.

The composition for eliminating hangover and liver function according to the present invention can effectively control the alcohol concentration in the blood by helping the activity of the alcohololytic enzyme and has an excellent effect for protecting the hepatocyte, And can be used easily and conveniently for improving hangover and liver function.

Figure 1 shows an animal modeling experiment to assess hangover efficacy.
FIG. 2 shows changes in blood alcohol concentration and ALDH by group according to time after administration of alcohol.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the present invention is not limited to the following Examples and Experimental Examples.

≪ Examples 1-3 >

The composition of Table 1 was mixed with dried green tea leaves, Chinese cabbage, Chinese cabbage, Chinese cabbage, citrus fruits, licorice root, Chinese cabbage, cinnamon, Chinese cabbage, red cabbage, red cabbage, red cabbage, red cabbage, An integer or constant of 10 times by weight was added thereto, and the mixture was extracted at a temperature of 105 DEG C for 2 to 10 hours. After the extraction was completed, it was separated from the extraction foil using a filter cloth, a stainless mesh net, and a dehydrator, and centrifuged or filtered using a cartridge filter.

Raw material name Example 1 Example 2 Example 3 Green tea 6.25 4 13.0 Gugija 6.25 10 8.0 An effective medicine 6.25 8 8.0 칡 6.25 10 8.0 Citrus 6.25 7 7.0 licorice 6.25 7 7.0 Chunmun Dong 6.25 6 6.0 Window plantain 6.25 6 6.0 cinnamon 6.25 6 6.0 Flower 6.25 10 5.0 Fried 6.25 5 5.0 Red ginseng 6.25 5 5.0 red bean 6.25 5 5.0 Boryeong 6.25 4 4.0 sign 6.25 4 4.0 ginger 6.25 3 3.0 Sum 100 100 100

<Experimental Example 1> Confirmation of hepatocyte protective activity

In order to confirm the effect of improving the liver function of the present invention, the above Examples 1 to 3 were treated with 300 mM of alcohol and then hepatocyte protective activity was confirmed by repeating 3 times. Specific experimental methods are as follows, and the experimental results are shown in Table 2;

Remove the medium of the flask in which the cells (over 80%) are cultured, and incubate for 2 minutes with trypsin. When the incubation is complete, tap the flask and drop the cells into a 15 mL falcon tube. An appropriate amount of medium is added to the tube and centrifuged at 15,000 rpm for 5 to 10 minutes. After centrifugation, the supernatant is discarded, and an appropriate amount of medium is pelleted by pelleting the pellet, and the cell count is adjusted by cell counting.

180 μL of the cell dilution is added to a 96-well plate and cultured in an incubator at 37 ° C and 5% CO 2 for 12 to 24 hours. The sample is then diluted with the solvent in which the sample is dissolved.

Add 20 μL of the diluted sample to a 96-well plate, incubate in an incubator at 37 ° C and 5% CO 2 for 2 hours, add 300 mM of 95% EtOH, and incubate for 24-48 hours.

MTT Add 20 μL of the reagent and incubate for 4 hours in an incubator at 37 ° C and 5% CO 2. After 4 hours, MTT reagent is flipped off and 200 μL of DMSO-Ethanol (1: 1) is added and incubated for 10 minutes. The absorbance is then measured at 570 to 630 nm.

Normal group Control group
(Treated with 300 mM EtOH) Example 1 (占 퐂 / mL) Example 2 ([mu] g / mL) Example 3 (占 퐂 / mL) 400 400 400 One 0.865 0.693 0.865 0.720 0.706 2 0.694 0.711 0.666 0.678 0.798 3 0.710 0.723 0.693 0.809 0.965 Average 0.756 0.709 0.741 0.736 0.823 Standard Deviation 0.094 0.015 0.108 0.067 0.131 Normal group
Contrast Activity (%) 100 93.742 98.017 97.268 108.814

&Lt; Experimental Example 2 >

In order to confirm the efficacy of the hangover resolution of the present invention, animal experiments using experimental rats were carried out.

The experimental group was divided into three groups. One group (C) was a control group and the second group (P) was a positive control group. After drinking alcohol, Hovenia japonica extract (250 mg / kg) as a functional ingredient was administered. Group 3 (AH) is the experimental group in which Example 3 (6.7 mg / kg) of the present invention was administered after alcohol consumption.

Blood alcohol and ALDH activity were measured at intervals of 30 minutes for a total of 300 minutes by dissecting and collecting blood after 30 minutes of oral administration of 20% alcohol 8 ml / kg once to experimental rats. The results are shown in FIG.

2-1. Check your blood alcohol level

The time to reach the maximum concentration after alcohol administration was 60 minutes in group C and P, and the alcohol concentration in AH group was much lower than that of group C and P. All groups recovered to levels before alcohol was administered at 300 min. The blood alcohol concentration at 60 minutes after alcohol administration was found to be decreased to 64.12 ± 9.37 ng / mL in Group C, and 36.14 ± 15.83 ng / mL in AH group according to the present invention, which was decreased to p <0.05. P group was 43.38 ± 10.75 ng / mL, which showed no significant difference but decreased. Thus, it can be seen that the blood alcohol concentration can be effectively controlled upon administration of Example 3 according to the present invention.

2.2 Identification of ALDH (aldehyde dehydrogenase) activity

At 60 minutes after alcohol administration, the activity of ALDH was 9.32 ± 2.44 milliunits / mL in group C and 14.60 ± 2.97 and 13.73 ± 3.66 milliunits / mL in group P and AH, respectively, &Lt; 0.05. &Lt; / RTI &gt; As a result, when Example 3 according to the present invention was administered, the ALDH activity of the liver tissue was increased. These results suggest that the present invention promotes the metabolism of alcohol by assisting the activity of the enzyme that decomposes alcohol.

Claims (6)

A composition for relieving hangover and improving liver function comprising a green tea extract,
The composition contains 13% by weight of the green tea extract as a total weight, 8% by weight of the extract of Gumijia, 8% by weight of the extract, 8% by weight of the extract, 7% by weight of citrus extract, 7% by weight of the licorice extract, 6% by weight of Cinnamonopsis lanceolata extract, 6% by weight of cinnamon extract, 5% by weight of chrysanthemum extract, 5% by weight of bamboo shoot extract, 5% by weight of red ginseng extract, 5% by weight of red bean extract, 4% And 3% by weight of a ginger extract. delete delete delete delete A health functional food composition for improving hangover resolution and liver function comprising the composition of claim 1.

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