KR101951812B1 - Cosmetic composition for heavy metal chelation and particulate matter removal comprising dianthus chinensis l. extract as active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for removing heavy metals and fine dusts containing Dianthus chinensis L. extract as an active ingredient. More particularly, the present invention relates to a cosmetic composition for removing microorganisms, A collagen biosynthesis inhibiting effect, a hyaluronic acid decomposition inhibiting effect, and a skin damage recovery effect, thereby suppressing the trouble phenomenon. The cosmetic composition according to the present invention has a feature of preventing the skin trouble caused by heavy metals and fine dusts by containing the extract of Gout.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition for removing heavy metals and fine dusts containing an extract of tofu as an active ingredient,

TECHNICAL FIELD The present invention relates to a cosmetic composition for removing heavy metals and fine dusts containing an extract of Tofu, which is characterized by inhibiting skin aging and skin troubles caused by fine dust and heavy metals.

Fine dust is a very small material that is invisible to the naked eye and means a particulate material with a diameter of 10 μm or less that floats or drifts in the air for a long time. Specifically, it is an air pollutant which is generated when fossil fuels such as coal and petroleum are burned, or when they come out from exhaust gas such as manufacturing / automobile exhaust gas, and are adsorbed to the lungs through the bronchi and cause various lung diseases.

Dust can be classified into total dust (TSP, total suspended particles), fine dusts with a diameter of 10 μm or less (PM 10), and ultrafine dusts with a diameter of 2.5 μm or less (PM 2.5) depending on the particle size. Fine dust is composed of ionic compounds such as nitrate (NO ₃ ^- ), ammonium (NH ₄ ⁺ ) and sulfate (SO ₄ ^2- ), carbon compounds and elements. ) Has designated BC (black carbon) emitted from diesel in fine dust as a first-level carcinogen. When exposed to fine dust for a long period of time, the immunity is rapidly lowered, and it can be exposed to various diseases such as cardiovascular diseases, skin diseases and eye diseases as well as respiratory diseases such as colds, asthma and bronchitis. Ultrafine dusts with a diameter of less than 2.5 μm are particularly susceptible to penetration into the bronchi and deep lungs of the human body, resulting in various diseases caused by bronchial and lung diseases (PM 2.5).

Dusts are sand in China's Gobi Desert and Mongolia, mainly in spring in March. On the other hand, the fine dust classified as a first-level carcinogen by the World Health Organization (WHO) is a high concentration of air pollutants including soot and automobile exhaust gas in Shanghai and Qingdao, China.

Fine dust affects not only inflammatory skin diseases such as psoriasis, atopic dermatitis and acne but also skin aging, hair loss and skin cancer. Fine dust can penetrate easily into the skin because it is up to 20 times smaller than the size of the pores, and persistent fine dust attack can lead to serious disease by losing protection of the skin barrier. It is also known that the increase of inflammatory cytokines and the increase of reactive oxygen species by fine dust increase inflammation reaction, wrinkle formation and pigmentation in skin cells, and seriously affect skin cancer outbreak. Fine dust can cause premature aging, moisture loss, pigmentation, wrinkles and wrinkles in the short term, loss of skin protection from environmental factors due to irreversible skin damage in the long term, It can cause illness.

Korean Patent No. 10-1629706 discloses a method for producing yellow dust or yellow dust containing at least one extract selected from the group consisting of Citrus sunki crustacean extract, Sceptridium ternatum or Botrychium ternatum extract and Korthalsella japonica extract as an active ingredient, The present invention relates to a composition for relieving skin inflammation caused by fine dusts. Korean Patent Laid-Open No. 10-2016-0108015 discloses a composition for preventing or treating skin damage caused by heavy metals containing as an active ingredient a complex plant extract of Guava, Sasae, Ulgum, Baeksoo, and Ishimaki .

Korean Patent No. 10-1177547 discloses a composition for preventing and treating skin diseases containing, as an active ingredient, one or more plant extracts selected from the group consisting of angular axis, 2006-137742 describes an anti-aging agent containing an extract of a plant of the genus Dianthus .

The inventors of the present invention have studied the applicability of various natural products to cosmetics as far as they are unknown, and found that the extract of the extract is selected and the effect of adsorbing heavy metal and fine dust, the effect of inhibiting cell death by fine dust, , The effect of inhibiting collagen synthesis by fine dusts, the effect of inhibiting the decomposition of hyaluronic acid due to fine dust, and the effect of recovering skin damages are excellent, and thus it is possible to expect the efficacy as a cosmetic product .

It is an object of the present invention to provide a method for inhibiting the collagen synthesis inhibition by fine dust, a method for inhibiting collagen synthesis by fine dust, A cosmetic composition exhibiting a hyaluronic acid decomposition inhibiting effect and a skin damage recovery effect.

It is also an object of the present invention to provide a cosmetic composition which exhibits an efficacy by preparing a supercritical, ultrasonic and fermented extract and purifying the extract in order to maximize the effectiveness of the extract.

TECHNICAL FIELD The present invention relates to a cosmetic composition for removing heavy metals and fine dusts containing a gingko extract as an active ingredient.

Preferably, the cosmetic composition has an effect of inhibiting cell death by fine dust, an inhibitory effect on inflammatory cytokine expression, an inhibitory effect on inhibition of collagen biosynthesis, an inhibitory effect on hyaluronic acid decomposition, or a skin damage recovery effect.

Preferably, the tofu extract is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

Preferably, the extract is selected from the group consisting of water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, And a solvent extraction method using an extraction solvent selected from the group consisting of a mixture of water and a solvent.

Preferably, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray ≪ / RTI >

The extract of the present invention showed the effect of inhibiting the expression of heavy metals and fine dust (Experimental Example 1), the effect of inhibiting cell death by fine dust (Experimental Example 2), the effect of inhibiting the expression of inflammatory cytokines by fine dust (Experimental Example 3) (Experimental Example 4), a hyaluronic acid decomposition inhibitory effect (Experimental Example 5) by micro dust, and a skin damaging effect (Experimental Example 6) by fine dust.

1 is a graph showing the amount of hyaluronic acid synthesized by Production Example 1 according to the present invention.

Gumaek used as an active ingredient of the cosmetic composition of the present invention (Dianthus chinensis L. ) extracts are used to impart the function of inhibiting skin troubles that may be caused by heavy metals and fine dusts.

Dianthus chinensis L. is a name derived from the shape of a seed and the shape of a seed similar to barley, which means a shape that grows in many directions. It is the top part of Dianthus chinensis L or Dianthus superbus L. It is odorless, flavorful, and natural. It is used for cystitis, urethritis, acute pyelonephritis, etc. It is also used for menstrual occlusion, menstrual cramps, menstrual cramps, and other diseases. It is also used for gingiva, gonorrhea, dysentery, and sore throat.

Diuretic action, intestinal excitatory action, cardiac inhibitory action, blood pressure lowering action, and inhibition of liver dyspnoea have been reported as pharmacological actions.

Its appearance is stalked, and there are branches with slightly elevated nodes, and the branches of the lanceolate are opposite to each other. Branches are branched here. Outer surface is yellowish green or light green with no hair. There is a flower or a stem at the end of the stem and branch. Calyx is cylindrical and its tip is divided into 5 branches, yellowish green, with its fruit on top. The seeds are brown. Other names include giant mackerel, mackerel, mackerel, southern sheepskins, large chrysanthemums, large orchids, mackerel, , Mountain beetle, and stone bamboo.

The gut extract of the present invention is obtained from the ground part (for example, flowers, stems, branches, etc.) of the phlox, and preferably means from stem or branch.

In the present invention, the extract of a plant for blocking heavy metals and fine dusts is obtained by extracting from various organs or parts (e.g., leaves, branches, flowers, roots, stems, fruits, etc.) of a plant, Or an extract from a branch.

In the present invention, the gut extract can be produced by using a conventional solvent according to a conventional method known in the art, that is, under the conditions of ordinary temperature and pressure, but preferably (a) water, Propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane (such as methanol, ethanol, , Chloroform, hexane, and a mixture thereof.

In the present invention, a solvent extraction method using an extraction solvent is preferably used. Preferably ethanol, 70% (v / v) ethanol or water, and most preferably 70% (v / v) ethanol.

On the other hand, it is obvious to those skilled in the art that the extract of the extract of the present invention having substantially the same effect can be obtained not only by the above-mentioned extraction solvent but also by using other extraction solvent.

The tofu extract according to the present invention includes an extract obtained through fermentation, and a fermented extract of a natural plant can be prepared as follows. The natural plant extract is finely crushed to a size of about 100 to 500 mesh, then 1 to 50 g / L of a conventional microorganism culture solution is added, and microorganisms such as yeast strains or lactic acid bacteria are added in an amount of 10,000 to 100,000 cfu / L. The culture temperature is 30 to 37 ° C. The pH is 5 to 7 and aerobic or anaerobic conditions are maintained for about 5 to 10 days. Which can be obtained through aging and filtration.

According to the present invention, the tofu extract is contained in an amount of 0.0001 to 30.0 wt% based on the total weight of the cosmetic composition, and more preferably, the tofu extract is contained in an amount of 0.01 to 10 wt% do. When the content of the extract is less than 0.0001% by weight, the effect of improving the skin is not exhibited. When the content of the extract is more than 30.0% by weight, the degree of improvement of the skin against the increase of the content is insignificant, It is not even.

Therefore, the cosmetic composition of the present invention comprising the extract of Gouraud having various functions is effective for removing heavy metals and fine dusts. In addition, the cosmetic composition according to the present invention prevents skin inflammation caused by heavy metals and fine dusts and restores skin damage. Specifically, the cosmetic composition for removing heavy metals and fine dusts can be obtained through excellent heavy metal and fine dust adsorption effect, cell death suppression effect, anti-inflammatory effect, and skin damage recovery effect.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Production Example 1: Preparation of Gingko Extract

1 kg of dried limbs (branches, stems or leaves) was precipitated in an aqueous solution of ethyl alcohol and water mixed at 7: 3 (v / v) for 5 days, filtered through 300 mesh, ≪ / RTI > The thus-obtained extract was easily dissolved in 1,3-butylene glycol so that the extract was obtained.

Experimental Example  1: Heavy metal and fine dust adsorption effect

In order to confirm the effect of heavy metal and fine dust adsorption on the extract of goopene, 100 ppm of lead, cadmium, copper, arsenic, zinc and fine dust (PM2.5) were reacted for 1 hour with 10,000 ppm concentration of Preparation Example 1, Respectively. The remaining heavy metal and fine dust content of the filter liquid remaining after filtration was measured by atomic absorption spectrometry. As a control group, a group containing purified water was used instead of the goat extract. The adsorption rate was calculated by dividing the concentration of the sample after the adsorption of the heavy metal and the fine dust by the concentration of the adsorbed sample. The higher the adsorption rate, the more effective adsorption and removal of the heavy metal and the fine dust were, and the results are shown in Table 1.

sample Adsorption (%) lead Copper cadmium zinc arsenic fine dust (PM2.5) Control group (no addition) - - - - - - Production Example 1 35 36 39 36 38 37

As can be seen from the results of Table 1, Production Example 1 of the present invention shows that the effect of adsorbing heavy metals and fine dusts is excellent.

Experimental Example 2: Effect of inhibiting cell death by fine dust particles

Experimental Example 2 is a measurement of the effect of inhibiting the cell death by the fine dust of the extract obtained in Production Example 1.

Human dermal keratinocytes (Korean Cell Line Bank, Korea) were inoculated into 48-well microplates (Nunc, Denmark) at a density of 1 × 10 ⁴ cells per well and cultured in DMEM medium (Sigma, Lt; / RTI > After the culture, the cells were further cultured for 24 hours in serum-free DMEM medium supplemented with fine dust (100 ppm) or yellow powder and 500 ppm of the extract of Preparation Example 1, respectively, and serum-free DMEM medium containing only fine dust as a control. The particulate matter used is all suspended particulate matter (TSP), particulate matter 2.5, particulate matter 10, and urban dust (Standard Reference Material ^®) 1649b). After incubation, MTT reagent was added and incubated for 4 hours. After the culture was removed, 1N NaOH / isopropanol solution was added and stirred for 20 minutes. Absorbance was measured at 565 nm using a spectrophotometer and the cell survival rate was calculated. Are shown in Table 2.

Cell survival rate (%) Yellow sand -0.01% PM2.5 -0.01% PM10 -0.01% urban dust-0.01% TSP -0.01% Control group (no addition) 43.7 35.5 49.8 46.4 41.5 Production Example 1 78.5 76.4 77.2 76.1 75.3

As can be seen from the results in Table 2, it was confirmed that apoptosis of skin keratinocytes was induced by yellow dust, TSP (total suspended particle), PM2.5 (Particulate matter 2.5), PM10 (Particulate matter 10) I could. It was confirmed that the extract prepared according to Preparation Example 1 of the present invention can inhibit the cell death caused by fine dusts in the keratinocyte, thereby increasing the cell survival rate. The gout extract of the present invention can reduce skin irritation that may occur with yellow sand and fine dust.

Experimental Example 3: Inhibitory effect of inflammatory cytokine on fine dust

Experimental Example 3 was conducted to evaluate the inhibitory effect of the extract obtained through Preparation Example 1 on the expression of inflammatory cytokines expressed by fine dust and heavy metals. Fibroblasts isolated from human epidermal tissue were placed in a 24-well test plate at 5 × 10 ⁴ and adhered for 24 hours. Each well was washed once with PBS and 500 占 퐇 of PBS was added to each well. The fibroblasts were further cultured in serum-free DMEM medium supplemented with fine dust (100 ppm) or yellow dust and 500 ppm of Preparation Example 1, respectively, and serum-free DMEM medium containing only fine dust for 24 hours as a control. 150 [mu] l of the culture supernatant was quantitated to determine the effect of inhibiting the expression of inflammatory cytokines in the extract of goat. The amount of IL-1α was quantitated using an enzyme-linked immunosorbent assay, and the inhibition rate (%) of inflammatory cytokine (IL-1α) was calculated by the following equation.

Bo: IL-1? Production in wells without UV irradiation

Bt: IL-1? Production in wells irradiated with ultraviolet light and not treated with sample

St: IL-1alpha production amount of well irradiated and treated with UV

Name of sample Inhibitory rate of inflammatory cytokine expression (%) Yellow sand (0.01%) PM2.5 (0.01%) PM10 (0.01%) Production Example 1 52.35 + 1.31 52.56 ± 1.43 53.94 + 1.37 Indomethacin (100 ppm) 62.58 ± 1.21 63.60 ± 1.07 61.72 + -0.94

According to Table 3, it can be seen that the extract of Preparation Example 1 inhibits the production of IL-1? Which is an inflammatory cytokine caused by fine dusts. Therefore, it can be seen that the extract of the present invention 1 effectively inhibits the inflammation caused by ultraviolet irradiation even at a low concentration.

Experimental Example 4: Inhibitory effect of collagen biosynthesis inhibited by fine dust particles

In Experimental Example 4, the effect of inhibiting collagen biosynthesis inhibition by the fine dust of the extract obtained in Production Example 1 was measured.

Human normal epithelial cells, fibroblasts, were inoculated on a 48-well microplate at a density of 1 x 10 ⁴ cells per well and cultured in DMEM medium for 24 hours. After the cultivation, the cells were further cultured for 24 hours in serum-free DMEM medium supplemented with fine dust (100 ppm) or yellow powder and 500 ppm of the extract of Preparation Example 1, respectively, and serum-free DMEM medium containing only dust or sand dust alone as a control. After the incubation, the supernatant of each well was collected, and the amount of procollagen type I C-peptide (PICP) was measured using a collagen kit (Takara, Japan) and converted into ng / Respectively. Collagen biosynthesis increase rate (%) was calculated according to the following formula (2), and the results are shown in Table 4.

Treatment concentration (% by weight) Increase in collagen biosynthesis (%) Yellow sand (0.01%) PM2.5 (0.01%) PM10 (0.01%) Production Example 1 65.2 63.5 63.1 Control group (no preparation / fine dust treatment) 21.4 22.1 24.5 Fine dust treatment 100 100 100

According to Table 4, it can be confirmed that the extract prepared according to Production Example 1 of the present invention recovers collagen biosynthesis degraded by fine dust.

Experimental Example 5: Inhibitory effect of micro dust on decomposition of hyaluronic acid

Experimental Example 5 was conducted to confirm that the extract of Preparation Example 1 can inhibit degradation of hyaluronic acid by fine dust. Human fibroblasts were cultured in DMEM containing 10% FBS at a concentration of 2 × 10 ⁴ cells / well in a 24-well plate. After 24 hours, the cells grown to 70-80% were replaced with serum-free DMEM and cultured for 5 hours. After culturing for 5 hours, the extracts of Preparation Example 1 were each treated with 500 ppm. Three days after the culture, the amount of total hyaluronic acid in the medium was measured by using a Hyaluronan Quantikine ELISA kit (R & D biosystem), and the result was shown in FIG.

1, it can be confirmed that the extract prepared by Production Example 1 of the present invention inhibits the decomposition of hyaluronic acid by fine dusts.

Formulation Example 1: Preparation of cosmetic composition containing extract for fine dust removal

A cosmetic composition containing the extract of Preparation Example 1 (Formulation Example 1) was prepared, and a cosmetic composition (Comparative Formulation Example 1) containing ceramide was used instead of the extract (Comparative Formulation Example 2) were prepared with the compositions shown in Table 5 below.

(Unit: wt%) ingredient Formulation Example 1 Comparative Formulation Example 1 Comparative Formulation Example 2 A Production Example 1 2 - - Ceramide - 2 - glycerin - - - 1,3-butylene glycol - - - EDTA-2Na 0.02 0.02 0.02 Purified water to100 to100 to100 B Cetostearyl alcohol 2 2 2 Glyceryl stearate 1.5 1.5 1.5 Microcrystalline 0.7 0.7 0.7 Squalane 5 5 5 Liquid paraffin 3 3 3 Trioctanoin 5 5 5 Polysorbate 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 Tocopheryl acetate 0.2 0.2 0.2 Cyclomethicone 3 3 3 BHT 0.1 0.1 0.1 C Incense, preservative Suitable amount Suitable amount Suitable amount

Experimental Example  6: Recovery of skin damage

TEWL (Trans-Epidermal Water Loss) is an index of moisture (excluding sweat) that evaporates through the skin and is used to assess the extent of damage to the stratum corneum and skin barrier function. A high level of TEWL indicates that the skin barrier is damaged. TEWL was measured at constant temperature (22 ° C) and humidity (47%), and the unit was g / h / m ² . The method of measurement was as follows: First, 30 times of tape-stripping was performed to cause damage to the skin barrier. TEWL levels were measured using a Tewameter TM210 (Courage + Khazaka, Germany) prior to application of the test product after causing skin barrier damage. Formulation Example 1 and Comparative Formulation Examples 1 and 2 of Table 5 were applied to damaged skin for 2 days twice a day for 3 days, and the change in TEWL value was measured to measure the degree of recovery of transdermal water loss according to Equation (3). The results are shown in Table 6.

Name of sample TEWL value TEWL Recovery (%) Before stripping After stripping Three days after application Formulation Example 1 9.18 19.44 11.23 80.02 Comparative Formulation Example 1 8.40 17.20 9.42 88.41 Comparative Formulation Example 2 9.38 18.61 15.05 38.57

As shown in Table 6, when TEWL was measured after applying Formulation Example 1 containing extract, Comparative Formulation Example 1 containing ceramide, and Comparative Formulation Example 2 containing no active ingredient, It can be seen that the cosmetic composition containing Example 1 quickly restores skin damage.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (5)

A cosmetic composition for the removal of heavy metals and fine dusts, comprising the extract of tofu as an active ingredient, wherein the extract is a top part of a fennel, and the extract of the extract is extracted with ethyl alcohol. The cosmetic composition according to claim 1, wherein the cosmetic composition is used for removing heavy metals and fine dusts having an effect of inhibiting cell death by fine dust, an effect of inhibiting inflammatory cytokine expression, inhibiting collagen biosynthesis inhibiting effect, hyaluronic acid decomposition inhibiting effect, Cosmetic composition. The cosmetic composition for removing heavy metals and fine dust according to claim 1, wherein the extract is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray Wherein the cosmetic composition has a formulation selected from the group consisting of: delete

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