KR102210655B1 - Cosmetic Composition For Heavy Metal Chelation And Particulate Matter Removal Comprising Extract Of Inulae Flos As Active Ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for removing heavy metals and fine dust, and contains a sunbokhwa solvent extract, a distribution extract or a fermented extract as an active ingredient.
The cosmetic composition according to the present invention has an effect of inhibiting apoptosis by fine dust, an effect of inhibiting the expression of inflammatory cytokines, an effect of inhibiting the inhibition of collagen biosynthesis, an effect of inhibiting the degradation of hyaluronic acid, and an effect of recovering skin damage.

Description

Cosmetic Composition For Heavy Metal Chelation And Particulate Matter Removal Comprising Extract Of Inulae Flos As Active Ingredient}

The present invention relates to a cosmetic composition for removing heavy metals and fine dust containing sunbokhwa extract as an active ingredient, and more particularly, an effect of inhibiting apoptosis by fine dust, an effect of suppressing the expression of inflammatory cytokines by fine dust, fine dust It relates to a cosmetic composition for removing heavy metals and fine dust having an effect of inhibiting collagen biosynthesis by inhibiting, inhibiting hyaluronic acid decomposition by fine dust, and recovering skin damage by fine dust.

Fine dust is a very small substance that is invisible to our eyes and means particulate matter with a diameter of 10㎛ or less that floats or scatters in the air for a long time. Specifically, it is an air pollutant that occurs when fossil fuels such as coal and petroleum are burned or from exhaust gases such as manufacturing and automobile exhaust, and adsorbed to the lungs through the bronchi, causing various lung diseases.

Dust is divided into total dust (TSP, Total Suspended Particle), fine dust with a diameter of 10 μm or less (PM 10), and ultrafine dust with a diameter of 2.5 μm or less (PM 2.5). Fine dust is nitrate (NO ₃ ^-), ammonium (NH ₄ ^+), sulfate (SO ₄ ^2-) there consists of ions, such as carbon compounds (carbon compounds), metal (elements) compound such as the World Health Organization (WHO ) Has designated BC (black carbon) emitted from diesel among fine dust as a first-class carcinogen. When exposed to fine dust for a long period of time, immunity is rapidly lowered, leading to exposure to various diseases such as cardiovascular disease, skin disease, and eye disease, as well as respiratory diseases such as colds, asthma, and bronchitis. Particularly, ultrafine dust with a diameter of 2.5㎛ or less easily penetrates deep into the bronchi and lungs in the human body and attaches to the bronchi and lungs, causing various diseases (PM 2.5). Yellow sand refers to the sand that comes mainly from the Gobi Desert in China and Mongolia in the spring of March. On the other hand, fine dust, which has been designated as a first-class carcinogen by the World Health Organization (WHO), is a high-concentration air pollutant in Shanghai and Qingdao, China, including soot and automobile exhaust.

Fine dust affects not only inflammatory skin diseases such as psoriasis, atopic dermatitis, and acne, but also skin aging, hair loss, and skin cancer. Since fine dust is up to 20 times smaller than the size of pores, it can easily penetrate into the skin, and continuous attack of fine dust may cause serious diseases by losing the protective function of the skin barrier. In addition, it is known that the increase of inflammatory cytokines and the increase of free radicals in the body caused by fine dust increase the inflammatory response, wrinkle formation, and pigmentation in skin cells, and seriously affect the development of skin cancer. Fine dust increases premature aging, lack of moisture, pigmentation, fine lines and wrinkles in the short term, and in the long term, irreversible skin damage causes the skin to lose its ability to protect the body from environmental factors, leading to serious problems such as skin cancer. May cause illness.

Korean Patent Registration No. 10-1629706 describes a composition for relieving skin inflammation caused by yellow dust or fine dust, comprising as an active ingredient at least one extract selected from the group consisting of a Tangerine peel extract, a bracken ginseng extract, and a white parasitic extract. . In addition, Korean Patent Laid-Open Publication No. 10-2016-0108015 describes a composition for preventing or treating skin damage caused by heavy metals containing complex plant extracts of guava, chorizo, turmeric, baekshou, and island Ogapi as active ingredients. .

As a result of researching the possibility of application to cosmetics in various natural products, the inventors of the present invention selected Seonbokhwa and prepared Seonbokhwa extract, distribution extract, and fermented extract from it to prevent heavy metal and fine dust adsorption effect and fine dust. The effect of measuring the effect of inhibiting apoptosis due to apoptosis, the effect of inhibiting inflammatory cytokines by fine dust, the effect of inhibiting the inhibition of collagen synthesis by fine dust, the effect of inhibiting the decomposition of hyaluronic acid by the fine dust, and the effect of recovering skin damage by the fine dust is very effective. I found it to be excellent.

The object of the present invention is to contain the extract, distribution extract and fermentation extract, so as to adsorb heavy metals and fine dust, inhibit apoptosis by fine dust, inhibit inflammatory cytokines by fine dust, inhibit collagen synthesis by fine dust. It is to provide a cosmetic composition that exhibits an inhibitory effect, an effect of inhibiting the decomposition of hyaluronic acid by fine dust, and an effect of recovering skin damage by fine dust.

Accordingly, the object of the present invention as described above is achieved by a cosmetic composition for removing heavy metals and fine dust containing an extract of Inulae Flos as an active ingredient.

Preferably, the sunbokhwa extract is (a) water, anhydrous or water-containing lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane , Hexane, and a solvent extract extracted with an extraction solvent selected from the group consisting of mixtures thereof, (b) the extract extracted by solvent extraction method of sunbokhwa, N-hexane, methylene chloride, ethyl acetate (Ethyl acetate) and a partitioned extract obtained by sequentially fractionating with water or (c) a fermented extract extracted using a fermentation extraction method obtained by fermentation with Lactobacillus plantarum ATCC 10012; and It is characterized in that it is a cosmetic composition for removing fine dust.

Preferably, the sunbokhwa extract is characterized in that it is a cosmetic composition for removing heavy metals and fine dust having an effect of inhibiting apoptosis by fine dust, an inhibitory effect of inhibiting collagen biosynthesis, an effect of inhibiting decomposition of hyaluronic acid, and an effect of recovering skin damage.

Preferably, the sunbokhwa extract is characterized in that it is a cosmetic composition for removing heavy metals and fine dust containing 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

Preferably, the cosmetic composition is a group consisting of a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a cleansing, an oil, a powder foundation, an emulsion foundation, a wax foundation, a pack, a massage cream and a spray. It is characterized in that it is a cosmetic composition for removing heavy metals and fine dust having a formulation selected from.

Seonbokhwa extract, distribution extract, and fermentation extract according to the present invention have the effect of adsorbing heavy metals and fine dust (Experimental Example 1), inhibiting apoptosis by fine dust (Experimental Example 2), and inhibiting the expression of inflammatory cytokines by fine dust. (Experimental Example 3), the effect of inhibiting the inhibition of collagen biosynthesis by fine dust (Experimental Example 4), the effect of inhibiting the decomposition of hyaluronic acid by fine dust (Experimental Example 5), and the effect of recovering skin damage due to fine dust (Experimental Example 6). .

Figure 1 shows the manufacturing process of the extract of Seonbokhwa distribution according to the present invention.
Figure 2 is a graph showing the hyaluronic acid decomposition inhibitory effect of the sunbokhwa extract according to the present invention.

Hereinafter, the present invention will be described in detail.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and correspond to the meanings as commonly understood by one of ordinary skill in the relevant fields of the present invention. In addition, although preferred methods or samples are described in the present specification, those similar or equivalent are included in the scope of the present invention.

The term “about” refers to 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, or 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, or relative to a reference amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length. It means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length varying by 4, 3, 2 or 1%.

Throughout this specification, unless otherwise required by the context, the terms “comprise” and “comprising” include the steps or components presented, or groups of steps or components, but any other step or component, or It is to be understood that a step or group of components is not excluded.

The cosmetic composition according to the present invention is a cosmetic composition containing a sunbokhwa extract, a distribution extract, or a fermentation extract containing as an active ingredient for removing heavy metals and fine dust. The herbal name of Seonbokhwa is Inulae Flos, and it is the dried medicinal material of Inula britannica var. japonica(Thunb.) Franch. & Sav). The shape is flat or spherical and consists of several guns and is arranged in a tiled shape. Fragment of bract is grayish yellow in shape of small scales. There are traces of calyx at the bottom of the gun, and the fragment of bract and the outer surface of calyx are covered with white hair. There are many white hairs at the end of the ovary, and the length is about 5mm. Here is a small oval seed. Seonbokhwa has a peculiar smell, bitter taste, spicy and salty, and a little warm in nature. Seonbokhwa is used for sputum, cough, hiccups, belching, asthma, chronic bronchitis, acute pleurisy, and stuffy symptoms as if the chest is always full by circulating and releasing the lumps. As a pharmacological action, anticonvulsant and diuretic effects of the bronchi have been reported.

In the present invention, the extract of Seonbokhwa means obtained by extracting from various organs or parts of Seonbokhwa (eg, leaves, branches, flowers, roots, stems, etc.), and is preferably an extract obtained from outposts or roots, Most preferably, it means an extract obtained from outpost. The sunbokhwa extract includes a solvent extract, a distribution extract, or a ferment extract.

In the present invention, the solvent extract of sunbokhwa may be prepared according to a conventional method known in the art, that is, under the conditions of a conventional temperature and pressure, using a conventional solvent, preferably (a) Water, anhydrous or hydrated lower alcohols having 1-4 carbon atoms (e.g., methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate , Dichloromethane, chloroform, hexane, and extracted using a solvent extraction method using a solvent selected from the group consisting of a mixture thereof. In the present invention, preferably, a solvent extraction method using an extraction solvent was used. It is preferably obtained using ethanol, 70% (v/v) ethanol or water, and most preferably, it is obtained using 70% (v/v) ethanol. On the other hand, it is apparent to those skilled in the art that the extract of the natural plant of the present invention can obtain an extract exhibiting substantially the same effect even by using not only the above-described extraction solvent but also other extraction solvents.

The distribution extract of sunbokhwa of the present invention is prepared by sequentially fractionating the solvent extract with N-hexane, methylene chloride, ethyl acetate, and water according to polarity. The solvent extract was redissolved in water, and then fractionated 2 to 5 times by adding N-hexane in the same amount as water, and the N-hexane layer was separated and concentrated under reduced pressure to prepare an N-hexane fraction. Next, after removing N-hexane, the same amount of methylene chloride was added to the remaining water layer, fractionated 2 to 5 times, and methylene chloride was separated and concentrated under reduced pressure to prepare a methylene chloride fraction. Then, after removing methylene chloride, the same amount of ethyl acetate was added to the remaining water layer to fractionate 2 to 5 times, and after removing ethyl acetate, it was concentrated under reduced pressure to prepare an ethyl acetate fraction. The remaining water layer is freeze-dried to prepare a water fraction.

The fermented extract of Seonbokhwa of the present invention can be prepared as follows. After finely crushing the sunbokhwa to about 100-500 mesh, 1-30% by weight of sunbokhwa was added to a conventional microbial culture solution, and lactic acid bacteria were added in an amount of 10,000 ~ 10,000,000 cfu/mL. The culture temperature is 30~37℃ under normal microbial culture conditions. The pH is 5-7, and incubation is carried out for about 24 to 72 hours under aerobic or usually anaerobic conditions. It can then be obtained through aging and filtration.

According to the present invention, the sunbokhwa extract is contained in 0.0001 to 30.0% by weight with respect to the total weight of the cosmetic composition, more preferably, the sunbokhwa extract is contained in 0.01 to 10% by weight with respect to the total weight of the cosmetic composition It is characterized. When the content of the sunbokhwa extract is less than 0.0001% by weight, the skin improvement effect does not appear, and when it exceeds 30.0% by weight, the degree of increase in the skin improvement effect due to the increase in content is insignificant, and there is a problem with the safety and stability of the formulation. And it is not economical.

Therefore, the cosmetic composition of the present invention including the extract of sunbokhwa, distribution extract, and fermentation extract having these various functions is effective in removing heavy metals and fine dust. In addition, the cosmetic composition according to the present invention prevents skin inflammation and recovers skin damage caused by heavy metals and fine dust. Specifically, the cosmetic composition for removing heavy metals and fine dust may be achieved through excellent heavy metal and fine dust adsorption effect, apoptosis inhibitory effect, anti-inflammatory effect, and skin damage recovery effect.

Ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the active ingredients as an active ingredient. For example, conventional ingredients such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances It includes an adjuvant, and a carrier.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , Oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray may be formulated, but is not limited thereto. In more detail, it may be prepared in the form of a flexible lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as carrier components. I can.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, ethoxylated isostearyl alcohol, suspending agents such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Sex cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like can be used.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether.

In the case of cleansing the formulation of the present invention containing a surfactant, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid Amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters, and the like may be used.

When the cosmetic composition of the present invention is a soap, a cleansing formulation containing a surfactant, or a cleansing formulation containing no surfactant, it may be applied to the skin and then wiped off, removed, or washed with water. As a specific example, the soap is liquid soap, powder soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, and the cleansing formulation without surfactant is a cleansing cream , Cleansing lotion, cleansing water, and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for describing the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Preparation Example 1: Preparation of sunbokhwa extract

100 g of shredded and shaded outpost was refluxed three times for 5 hours each with 70% (V/V) ethanol aqueous solution, cooled, and then filtered through Whatman #4 filter paper. The filtered extract was concentrated under reduced pressure at 50° C. or lower and freeze-dried to obtain 19.4 g of sunbokhwa extract.

Preparation Examples 2 to 5: Fractions of Seonbokhwa extract

In order to fractionate the ethanol extract according to polarity, a systematic fractionation was performed as shown in FIG. 1 using an organic solvent. Specifically, after dissolving the ethanol extract in water again, the same amount of N-hexane was added, and the hexane layer was separated to obtain a hexane fraction (Preparation Example 2). An equal amount of methylene chloride was added to the water layer from which the hexane fraction was removed, and the methylene chloride layer was separated to obtain a methylene chloride fraction (Preparation Example 3). An equal amount of ethyl acetate was added to the water layer from which the methylene chloride fraction was removed, and the ethyl acetate layer was separated to obtain an ethyl acetate fraction (Preparation Example 4). The ethyl acetate fraction was removed and the remaining water layer was lyophilized to obtain a water fraction (Preparation Example 5). The fractionation process was performed at room temperature, and each fraction was allowed to stand for at least 3 hours. The process of obtaining each fraction was repeated twice, and the fractions excluding the water layer were filtered, concentrated under reduced pressure, and dried. The process is described in Figure 2.

Preparation Example 6: Preparation of fermented extract of Seonbokhwa

Preparation Example 6 was prepared by fermenting Preparation Example 1 as a fermented extract of Seonbokhwa extract. The culture medium for lactic acid bacteria was prepared by adding Lactobacillus MRS Broth (DIFCO, USA) to 1 to 30% by weight, preferably 10% by weight, respectively, of the Seonbokhwa extract of Preparation Example 1 . Lactobacillus plantarum ( Lactobacillus plantarum ATCC 10012) was inoculated with 10,000,000 cfu in the Lactobacillus MRS medium and cultured at 35° C. for 72 hours. After cultivation, the culture solution was centrifuged to remove bacteria to obtain a precipitated fermented product, and ethanol was added to the final 70% (V/V), followed by extraction under reflux 3 times for 5 hours, cooling, and then Whatman Filtered through (Whatman) #4 filter paper. The filtered fermented extract was filtered again with 0.25 um filter paper, concentrated under reduced pressure at 60° C. or lower, and then frozen to obtain 16.3 g of fermented fermented extract.

Experimental Example 1: Adsorption effect of heavy metals and fine dust

In order to confirm the adsorption effect of heavy metals and fine dust of natural plant extracts, 100 ppm of lead, cadmium, copper, arsenic, zinc, and fine dust (PM2.5) of 100 ppm were respectively added to Preparation Examples 1 to 6 at a concentration of 10,000 ppm for 1 hour, respectively. After reacting, it was filtered. The content of residual heavy metals and fine dust in the filter liquid remaining after filtering was measured by atomic absorption spectrometry. As a control, a group containing purified water was used instead of the preparation example. The adsorption rate was calculated by dividing the concentration of the sample after adsorption of heavy metals and fine dust by the concentration of the sample before adsorption. The higher the adsorption rate, the more effective it means to adsorb and remove heavy metals and fine dust, and the results are shown in Table 1.

sample Adsorption rate (%) lead Copper cadmium zinc arsenic fine dust (PM2.5) Control (no addition) - - - - - - Manufacturing Example 1 40 37 37 50 49 45 Manufacturing Example 2 51 49 50 50 52 51 Manufacturing Example 3 52 51 50 49 52 50 Manufacturing Example 4 50 49 51 49 51 49 Manufacturing Example 5 31 29 28 32 32 30 Manufacturing Example 6 55 52 52 52 54 53

As can be seen from the results of Table 1, the solvent extract prepared by Preparation Example 1, which is the sunbokhwa extract of the present invention, the distribution extract prepared by Preparation Examples 2 to 5, and the ferment extract prepared by Preparation Example 6, are heavy metals And it was confirmed that the fine dust adsorption effect was excellent.

Experimental Example 2: Effect of inhibiting apoptosis by fine dust

Experimental Example 2 measures the effect of inhibiting apoptosis by fine dust of the extracts obtained in Preparation Examples 1 to 6.

Human skin keratinocytes (Korea Cell Line Bank, Republic of Korea) were inoculated into 48-well microplates (Nunc, Denmark) to 1×10 ⁴ cells per well, and 24 at 37°C in DMEM medium (Sigma, USA). Incubated for hours. After cultivation, fine dust (100 ppm) or yellow sand and the extracts of Preparation Examples 1 to 6 were further cultured for 24 hours in a serum-free DMEM medium containing 500 ppm, respectively, and a serum-free DMEM medium containing only fine dust as a control. The fine dust used is yellow sand or all particulate matter with a diameter of 0.001㎛ floating in the air, such as TSP (total suspended particle), PM2.5 (Particulate matter 2.5), PM10 (Particulate matter 10) and urban dust (Standard Reference Material).1649b). After incubation, MTT reagent was added and incubated for 4 hours, the culture solution was removed, 1N NaOH/isopropanol solution was added, stirred for 20 minutes, absorbance was measured at 565 nm with an absorbance photometer, and the viability of cells was calculated. It is shown in Table 2.

Cell viability (%) Yellow sand (0.01%) PM2.5 (0.01%) PM10 (0.01%) urban dust (0.01%) TSP (0.01%) Control 40.5 31.3 38.4 40.3 37.5 (No additives) Manufacturing Example 1 73.4 75.5 75.4 78.1 73.8 Manufacturing Example 2 88.6 87.7 88.1 87.5 85.8 Manufacturing Example 3 86.7 85.6 86.3 86.1 84.9 Manufacturing Example 4 85.2 86.6 86.6 87.1 85.3 Manufacturing Example 5 69.5 68.4 67.8 68.1 66.4 Manufacturing Example 6 88.5 87.5 86.2 88.6 87.7

As can be seen from the results of Table 2, it was confirmed that apoptosis of skin keratinocytes was induced by yellow dust, TSP (total suspended particle), PM2.5 (Particulate matter 2.5), PM10 (Particulate matter 10), and urban dust. Could The extract prepared by Preparation Example 1 of the present invention, the fractions prepared by Preparation Examples 2 to 5, and the fermentation extract prepared by Preparation Example 6 suppress apoptosis caused by fine dust in skin keratinocytes It was confirmed that can be increased.

Experimental Example 3: Inhibitory Effect of Inflammatory Cytokine Expression by Fine Dust

Experimental Example 3 is to evaluate the effect of inhibiting the expression of inflammatory cytokines expressed by fine dust and heavy metals of the extracts obtained through Preparation Examples 1 to 6. Fibroblasts isolated from human epidermal tissue were placed in a 24-well test plate each of 5×10 ⁴ and adhered for 24 hours. Each well was washed once with PBS, and 500 μl of PBS was added to each well. The fibroblasts were further cultured for 24 hours in a serum-free DMEM medium containing 500 ppm of fine dust (100 ppm) or yellow dust and Preparation Examples 1 to 6, respectively, and a serum-free DMEM medium containing only fine dust as a control. By taking 150 μl of the culture supernatant and quantifying IL-1α, the inhibitory effect of plant extracts and mixtures on inflammatory cytokine expression was determined. The amount of IL-1α was quantified using an enzyme-linked immunosorbent assay, and the inhibition rate (%) of the expression of an inflammatory cytokine (IL-1α) was calculated by Equation 1 below.

Bo: IL-1α production in wells without UV irradiation and sample treatment

Bt: The amount of IL-1α produced in the well not subjected to UV irradiation and sample treatment

St: IL-1α production amount of the sample-treated well after irradiation with ultraviolet rays

Sample name Inhibition rate of inflammatory cytokine expression (%) Yellow sand (0.01%) PM2.5 (0.01%) PM10 (0.01%) Manufacturing Example 1 54.13 ± 1.46 52.17 ± 1.13 53.27 ± 1.06 Manufacturing Example 2 65.11 ± 1.87 66.18 ± 1.14 65.22 ± 1.37 Manufacturing Example 3 67.18 ± 1.24 66.24 ± 1.18 67.12 ± 1.31 Manufacturing Example 4 66.92 ± 1.21 67.53 ± 1.37 66.58 ± 1.22 Manufacturing Example 5 45.35 ± 1.29 43.14 ± 1.26 44.32 ± 1.16 Manufacturing Example 6 74.12 ± 1.31 73.26 ± 2.14 74.36 ± 1.32 Indomethacin (100ppm) 62.27 ± 1.27 63.29 ± 1.12 61.43 ± 0.87

According to Table 3, the extract prepared by Preparation Example 1, the fractions prepared by Preparation Examples 2 to 5, and the fermented extract prepared by Preparation Example 6 inhibit the production of IL-1α, an inflammatory cytokine by fine dust. I could see that.

Experimental Example 4: Inhibitory effect of inhibiting collagen biosynthesis by fine dust

In Experimental Example 4, the effect of inhibiting the inhibition of collagen biosynthesis by fine dust of the extracts obtained in Preparation Examples 1 to 6 was measured. Fibroblasts, which are human normal epithelial cells, were inoculated into 48-well microplates at 1 x 10 ⁴ cells per well, and cultured in DMEM medium for 24 hours. After cultivation, it was further cultured for 24 hours in a serum-free DMEM medium containing 500 ppm of fine dust (100 ppm) or yellow sand and the extracts of Preparation Examples 1 to 6, respectively, and a serum-free DMEM medium containing only fine dust or yellow sand as a control. . After incubation, the supernatant of each well was collected and the amount of procollagen-type IC-peptide (PICP) was measured using a collagen kit (Takara, Japan) and converted into ng/ml, and the amount of synthesized collagen was measured. I did. Collagen biosynthesis increase rate (%) was calculated according to Equation 2 below, and the results are shown in Table 4.

Treatment concentration (% by weight) Collagen biosynthesis rate (%) Yellow sand (0.01%) PM2.5 (0.01%) PM10 (0.01%) Manufacturing Example 1 65.8 66.8 62.5 Manufacturing Example 2 73.1 72.8 72.2 Manufacturing Example 3 74.6 72.4 73.7 Manufacturing Example 4 75.4 74.8 73.5 Manufacturing Example 5 54.8 52.8 50.5 Manufacturing Example 6 78.2 79.1 79.4 Control (Preparation example no addition/fine dust treatment) 20.4 21.2 23.7 No fine dust treatment 100 100 100

According to Table 4, the extract prepared by Preparation Example 1 of the present invention, the fractions prepared by Preparation Examples 2 to 5, and the fermented extract prepared by Preparation Example 6 restore collagen biosynthesis reduced by fine dust again. I could confirm that.

Experimental Example 5: Effect of inhibiting hyaluronic acid decomposition by fine dust

Experimental Example 5 was carried out to confirm whether the extracts of Preparation Examples 1 to 6 can suppress the decomposition of hyaluronic acid by fine dust. Human fibroblasts were cultured in DMEM containing 10% FBS, added to a 24-well plate at a concentration of 2×10 ⁴ cells/well, and cultured. After 24 hours, the medium of the cells grown to 70-80% was replaced with Serum-free DMEM and cultured for 5 hours. After incubation for 5 hours, the extracts of Preparation Examples 1 to 6 were each treated with 500 ppm. After 3 days of culture, the total amount of hyaluronic acid in the medium was measured using the Hyaluronan Quantikine ELISA Kit (R&D biosystem), and the results are shown in FIG. 1.

According to FIG. 1, it was confirmed that the extract prepared by Preparation Example 1 of the invention, the fractions prepared by Preparation Examples 2 to 5, and the fermented extract prepared by Preparation Example 6 inhibited the degradation of hyaluronic acid by fine dust. .

Formulation Example 1: Preparation of a cosmetic composition containing an extract for removing fine dust

To prepare a cosmetic composition (Formulation Examples 1 to 6) each containing the extract or fraction or fermentation extract of Preparation Examples 1 to 6, and a cosmetic composition containing ceramide as a moisturizer (Comparative Formulation Example 1), extracts or moisturizers all The cosmetic composition that does not contain (Comparative Formulation Example 2) is shown in Table 5 below.

ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Formulation Example 6 Comparative Formulation Example 1 Comparative Formulation Example 2 A Manufacturing Example 1 2 - - - - - - - Manufacturing Example 2 - 2 - - - - - - Manufacturing Example 3 - - 2 - - - - - Manufacturing Example 4 - - - 2 - - - - Manufacturing Example 5 - - - - 2 - - - Manufacturing Example 6 - - - - - 2 - - Ceramide - - - - - - 0.5 - glycerin - - - - - - - - 1,3 butylene glycol - - - - - - - - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Purified water to100 to100 to100 to100 to100 to 100 to 100 to 100 B Cetostearyl alcohol 2 2 2 2 2 2 2 2 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Microcrystalline 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 Squalane 5 5 5 5 5 5 5 5 Floating paraffin 3 3 3 3 3 3 3 3 Trioctanoine 5 5 5 5 5 5 5 5 Polysorbate 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl Acetate 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Cyclomethicone 3 3 3 3 3 3 3 3 BHT 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 C Fragrance, preservative Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount

Experimental Example 6: Skin damage recovery effect

TEWL (Trans-Epidermal Water Loss) is an index that indicates moisture evaporated through the skin (excluding sweat) and is used to evaluate the degree of damage to the stratum corneum and skin barrier function. A high level of TEWL indicates that the skin barrier is damaged. TEWL was measured at constant temperature (22°C) and humidity (47%), and the unit is g/h/m ² . The measurement method is as follows. First, tape-stripping was performed 30 times to induce damage to the skin barrier. After inducing damage to the skin barrier and before applying the test product, the TEWL value was measured using Tewameter TM210 (Courage+Khazaka, Germany). Formulation Examples 1 to 6 of Table 5 and Comparative Formulation Examples 1 and 2 of Table 5 were applied to the damaged skin twice a day for 3 days, and then the change in TEWL value was measured, and the degree of recovery of transdermal moisture loss was measured according to Equation 3. . The results are shown in Table 6.

Sample name TEWL value TEWL recovery (%) Before stripping After stripping 3 days after application Formulation Example 1 7.1 18.6 9.5 79.1 Formulation Example 2 7.4 18.9 9.3 83.5 Formulation Example 3 7.2 18.6 8.5 88.6 Formulation Example 4 7.2 18.5 8.8 85.8 Formulation Example 5 7.2 18.7 11.3 64.3 Formulation Example 6 7.3 18.4 7.8 95.5 Comparative Formulation Example 1 7 18.5 7.5 95.7 Comparative Formulation Example 2 7.1 19.5 15.8 29.8

As shown in Table 6, after application of Formulation Examples 1 to 6 containing extracts and mixtures and Comparative Formulation Example 1 containing ceramide and Comparative Formulation Example 2 containing no active ingredient to the skin, TEWL was measured to determine the skin damage recovery effect. As a result of confirmation, it can be seen that the cosmetic composition containing Formulation Examples 1 to 6 rapidly recovers skin damage.

As described above, specific parts of the present invention have been described in detail, and it is obvious that these specific techniques are only preferred embodiments for those of ordinary skill in the art, and the scope of the present invention is not limited thereto. Therefore, it will be said that the practical scope of the present invention is defined by the appended claims and their equivalents.

Claims (7)

As a cosmetic composition for removing heavy metals and fine dust containing Inulae Flos extract as an active ingredient,
The sunbokhwa extract is a distribution extract obtained by sequentially fractionating the extract obtained by extracting sunbokhwa by a solvent extraction method into N-hexane, methylene chloride, and ethyl acetate, or lactobacillus It is a fermented extract extracted using a fermentation extraction method obtained by fermenting with plantarum (Lactobacillus plantarum ATCC 10012),
The distribution extract is a fraction obtained by fractionation with N-hexane, a fraction obtained by fractionation with methylene chloride, or a fraction obtained by fractionation with ethyl acetate. Cosmetic composition for dust removal. The method of claim 1, wherein the extraction solvent used in the solvent extraction method is water, an anhydrous or hydrous lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether , Dichloromethane, hexane, and a cosmetic composition for removing heavy metals and fine dust, characterized in that the solvent extract extracted with an extraction solvent selected from the group consisting of a mixture thereof. The cosmetic composition of claim 1, wherein the sunbokhwa extract has an effect of inhibiting apoptosis by fine dust, inhibiting collagen biosynthesis, inhibiting hyaluronic acid decomposition, and recovering skin damage. The cosmetic composition for removing heavy metals and fine dust according to claim 1, wherein the sunbokhwa extract is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. The method of claim 1, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing, oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray. Having a formulation selected from the group consisting of, a cosmetic composition for removing heavy metals and fine dust. delete delete

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