KR101951330B1 - Device for monitoring quality of water - Google Patents

Abstract

The present invention relate to a water quality monitoring device using microalgae with improved structure, wherein a rotary table is used to rotate a measurement cell so as to inject an algae culture fluid and external sample water onto the measurement cell by using a syringe injector. Moreover, a camera in which an optical image sensor is installed is used to monitor a direct change value of toxicity in accordance with an optical image in real time. In addition, the syringe injector is washed in time between individual processes by using purified water to prevent pollution even though the syringe injector is repeatedly used. Therefore, errors in a toxicity measurement test can be reduced.

Description

TECHNICAL FIELD [0001] The present invention relates to a device for monitoring water quality using microalgae,

In particular, the present invention relates to an apparatus for monitoring water quality using microalgae. In particular, the present invention relates to an apparatus for monitoring water quality using microscopic algae, By monitoring the intuitive toxicity change value according to the optical image using the installed camera in real time and cleaning the syringe injector using purified water between each processes, it is possible to prevent contamination even by repeated use of the syringe injector, And more particularly to a water quality monitoring apparatus using microalgae whose structure is improved so as to reduce errors.

As a general environmental problem, water pollution is a problem. Thus, there is a growing interest in methods for measuring the toxicity of water.

Many devices for measuring the toxicity of existing water quality have been developed, but most have been limited to chemical water quality toxicity measuring devices.

The chemical water quality toxicity measuring device can be quantitatively measured only in a substance known to the water system, and it is disadvantageous in that it can be used only by a high-priced installation cost and a skilled technical researcher.

In recent years, development of a biological water quality toxicity measuring device has been under way to overcome such problems, and devices for measuring water quality toxicity using fish, daphnia, luminescent bacteria, microalgae and microbial cells have been developed.

Fish toxicity measuring device is a device that measures the toxicity of water by using the reflux that fish goes back to the water flow. However, since the size of the object to detect toxicity is large, the accuracy is low and the measurement time and error range are large. have.

When the water is not contaminated by toxic substances, the water daphnia shows regular movement. When toxic substances are introduced into the water daphnia, the movement of water daphnia changes irregularly and rapidly decreases However, there is a disadvantage that the culture of daphnia must be replaced two or three times a week, and it is troublesome to separate the mother and the baby every day.

Prior art related to a water quality monitoring apparatus using microalgae is disclosed in Korean Patent Laid-Open Publication No. 10-2015-0089402 entitled " Apparatus and Method for Measuring Water Quality Toxicity Using Algae "(Published Date: A culture solution container for culturing the culture solution necessary for culturing the culture medium; and a sample container for storing the effluent of the river water or the water treatment facility; A filter unit connected to the solution storage unit and filtering the suspended substances before transferring the water from the river water or the water treatment facility to the reserve tank; A solution transfer unit connected to the filter unit and supplying a culture solution from the solution storage unit to the reservoir tank and the photochemical sensor unit and supplying drainage water from the filter unit to the reservoir tank and the photochemical sensor unit, Wow; A photochemical sensor unit connected to the solution transfer unit and the central processing unit and equipped with a photochemical sensor capable of measuring a light quantity of a wavelength band of a visible light region and amplifying and digitizing an analog value of the measured light quantity; A signal communication unit connected to the central processing unit, for transmitting data digitized by the photochemical sensor unit to the terminal of the control center via wire / wireless, and for transmitting the command transmitted from the terminal of the control center to the central processing unit; A display unit connected to the central processing unit and displaying a difference between an analog light amount value measured from an algae exposed to the algae on the culture liquid and pollutants, an amplified digital value and a difference between the two values in real time in a graphical form; And a central processing unit connected to the photochemical sensor unit, the signal communication unit, the temperature regulating unit, and the display unit to operate an OS to control the change in chromaticity of the cells when the algae are exposed to the toxic substance, And the data of the measured chromaticity is calculated, and the water quality toxicity is calculated by using the data of the chromaticity change.

Prior art related to existing water quality monitoring apparatuses is disclosed in Japanese Laid Open Patent Publication No. 11326312 entitled " Water Quality Monitoring Device "(published on November 26, 1999) And the dissolved oxygen supply means is provided in the measurement chamber and includes a temperature sensor for detecting the temperature of the sample water and a heater installed in the sample water channel on the upstream side of the dissolved oxygen meter .

However, the conventional water quality monitoring apparatus is not only difficult to continuously measure the sample, but also may contaminate the injector during repeated measurement of the sample water and the algal culture liquid on the upper side of the measuring cell during the continuous measurement operation.

In addition, the existing water quality monitoring device analyzes the data based on the numerical value generated by digitizing the change of the fluorescence amount measured through the photochemical sensor, so that it is not possible to monitor in real time on the monitor, There is a disadvantage that the numerical value can not be obtained.

Korean Patent Laid-Open Publication No. 10-2015-0089402 "Apparatus and Method for Measuring Water Quality Toxicity Using Algae" (Publication Date: Aug. 20, 2015) Japanese Laid Open No. 11326312 "Water Quality Monitoring Device" (Open date: November 26, 1999)

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned problems, and an object of the present invention is to provide an algae culture liquid and an external sample water by rotating a measurement cell using a rotary table, In addition, by monitoring the intuitive toxicity change value according to the optical image in real time using a camera equipped with optical image sensor and cleaning the syringe injector between each process by using purified water, And to provide a water quality monitoring device using microalgae whose structure is improved so as to reduce the toxicity measurement test error.

In order to accomplish the above object, the present invention provides a method for controlling an internal combustion engine, comprising the steps of: dividing an internal space into an upper space and a lower space based on a rotary table, a sample tank containing a purified water tank and sample analysis water, A main body having a drainage tank; A rotary table arranged on the upper side of the lower space part so as to be rotatable by a predetermined angle by a driving unit and having a plurality of through holes so that an end of the syringe injector is lowered toward the lower space part when the syringe injector is lowered; A plurality of measurement cells arranged in the rotary table and rotatable by 180 ° such that they can be turned upside down by rotation means and arranged to perform different processes each time the rotary table is rotated at a predetermined angle, ; Wherein the syringe needle is provided so as to be vertically movable up and down so that the contents contained in the purified water tank, the sample tank and the culture tank can be respectively injected into the inside, A syringe injector for injecting sample analysis water and algae culture fluid into any one of the plurality of measurement cells; And an analyzer for analyzing the toxicity change of the sample analyzer, which is disposed in the upper hollow portion of the upper side of the rotary table and is installed so as to be able to ascend and descend by the ascending and descending means and mixed with the algae culture liquid contained in any one of the plurality of measurement cells, And a digital camera device for measuring the rotation angle of the rotary table and outputting the measurement value to a monitor, wherein the rotation means comprises: a fixed block provided on the upper side of the rotary table; And a fixing bracket provided on an upper side of the rotary table so that the other side of the measurement cell is connected to a connection pin, A guide block provided in a vertical direction; Teoro characterized in that configured.

The driving means includes a driving motor provided in the lower space portion and provided with a driving pulley at a lower portion thereof, a driven pulley provided at a lower portion of the rotating table and connected to the driving pulley so as to transmit rotational force to the rotating table, As shown in Fig.

The syringe injector includes at least two syringe injectors that simultaneously operate. The syringe injector sucks and discharges purified water into the syringe body when the sample analysis water or algae culture liquid is introduced into the receiving groove, thereby cleaning the syringe body To perform an operation.

And a cleaning nozzle installed in the water retention tank and discharging purified water for cleaning toward the inverted measurement cell.

The rotating table is rotated by an angle of 90 ° by the driving means, and the measuring cells are arranged in four on a rotating table so as to perform respective processes by rotating at an angle of 90 °.

The moving means includes a guide member installed in the upper hollow portion of the main body in a lateral direction, a guide member connected to the guide member by a connection bracket and supported by the first screw shaft and the guide bar, A first moving motor having a connecting block which moves left and right and a first screw shaft which is axially coupled to the connecting block and is driven to rotate in the forward and reverse directions in response to application of external power, A second moving motor supported by the first moving block and having a second screw shaft rotatably driven in the forward and reverse directions when external power is applied; A lift block supported on a first guide bar axially coupled to the first moving block and supported by the first moving block to be moved up and down during rotation of the second screw shaft, A second moving block coupled to the second moving block so as to be coupled to the syringe body of the syringe injector at a lower portion thereof to be coupled to the second moving block and having a third screw shaft rotatably driven in the forward and reverse directions A third moving motor, and a second guide bar which is axially coupled to the third screw shaft and is supported by the second moving block, is lifted and lowered when the third screw shaft is driven to rotate, and the piston of the syringe injector And a lifting plate connected to the lifting plate.

The present invention relates to a method and apparatus for intuitively detecting toxicity by using a digital camera apparatus equipped with an optical image sensor capable of imaging a toxicity change value while irradiating light to induce a chlorophyll fluorescence reaction in a state where sample analysis water supplied from an external source is mixed with an algal culture liquid It is possible not only to monitor the change value on the monitor in real time but also to perform the continuous measurement test automatically by using the rotation table, thereby obtaining more accurate test data.

In addition, since the rotating means of the present invention can rotate the measuring cell so as to be turned upside down by 180 ° by the driving rotational force of the rotating motor, the algae culture liquid and the sample analyzing water contained in the receiving groove can be easily poured into the water collecting tank, So that the inside of the receiving groove of the inverted measurement cell can be easily cleaned.

Since the four measurement cells of the present invention are rotated at an angle of 90 degrees by the driving means, the rotation table of the present invention can be used for washing the syringe body, analyzing the sample and injecting the algae culture liquid, mixing the sample analysis water and the algae culture liquid, Measurement, and cleaning process of the measuring cell can be performed successively, respectively.

Since the syringe needle can be lifted and lowered using the third moving motor as well as the upward / downward movement and the left / right movement of the syringe injector according to the present invention, since the syringe body is cleaned It is possible to inject the algae culture liquid and the sample analysis water into the first, second, third and fourth receiving grooves.

1 is an external perspective view of a water quality monitoring apparatus using microalgae according to the present invention.
2 is a perspective view showing the internal structure of a water quality monitoring apparatus using microalgae according to the present invention.
3 is a cross-sectional view showing the rotating table, the driving means and the rotating means of the present invention.
4 is a plan view showing the internal structure of the main body of the present invention.
5 is a plan view showing a rotation table and first, second, third and fourth measurement cells according to the present invention;
6 is a perspective view showing the syringe feeder and elevating means of the present invention.
7 is a perspective view showing a rotary table and a syringe injector of the present invention.
8 is a schematic structural view of the moving means of the present invention.
9 is a use state view of the moving means of the present invention.
FIG. 10 is a schematic view showing a cleaning process using purified water of a syringe injector of the present invention. FIG.
11 is a flow chart sequentially showing the operation of the syringe injector of the present invention.

1 to 11, the internal space is divided into an upper hollow portion 110 and a lower hollow portion 120 with reference to the rotary table 200, A main body 100 having a sample tank 30 in which a purified water tank 10 and a sample analysis water are contained, a culture tank 20 containing a algal culture liquid and a water retention tank 40 are installed in the lower space part 120; The lower end of the syringe injector 400 is connected to the upper end of the lower space 120 by a driving unit 600 at a predetermined angle, A rotation table 200 having a plurality of through holes 210 formed therein so as to be lowered; And is arranged to be rotatable by 180 ° so as to be turned upside down by the rotating means 700 and arranged to perform different processes at every rotation of the rotary table 200 at a predetermined angle, A plurality of measurement cells (300) having a groove (310); The syringe needle 410 is installed in the upper hollow section 110 and is moved leftward, rightward and upward and downward by the moving means 800 so that the syringe needle 410 can be elevated upward and downward. The purified water tank 10 and the sample tank A syringe injector 400 injecting the sample analysis water and the algal culture liquid into any one of the plurality of measurement cells 300, the syringe injector 400 being capable of injecting the contents contained in the culture tank 20 and the culture tank 20 into the respective measurement cells 300; And an algae culture liquid which is disposed in the upper hollow portion 110 on the upper side of the rotary table 200 and is vertically movable up and down by the elevating means 900 and is contained in any one of the plurality of measurement cells 300 during the lowering operation, And a digital camera device 500 for measuring the toxicity change of the mixed sample analysis water with the optical image sensor 510 and outputting the measured value to a monitor.

Referring to FIGS. 1 and 2, the main body 100 is formed in a cabinet shape. The main body 100 includes an upper space 110 provided on the upper side of the rotary table 200 with respect to the rotation table 200, And a lower space part 120 provided below the rotary table 200.

The main body 100 is provided with an inspection opening 150 which is rotated around the hinge to be opened and closed for an internal inspection.

The rotary table 200 is formed in a circular plate structure and a plurality of through holes 210 are formed so that the syringe of the syringe injector 400 is lowered into the lower space portion 120.

The through hole 210 has a structure formed in the shape of a long elongated hole at a position close to each measurement cell 300.

3, the driving means 600 for rotating the rotary table 200 includes a driving motor 610 installed in the lower space portion 120 and provided with a driving pulley 615 at a lower portion thereof, And a rotating shaft 620 provided at a lower portion of the rotating table 200 and having a driven pulley 625 connected to the driving pulley 615 by a belt so as to be rotated together with the driving pulley 615 to transmit rotational force to the rotating table 200 .

The driving unit 600 rotates the rotary table 200 by a predetermined angle of 90 ° so that the measuring cell 300 is rotated at an angle of 90 ° to rotate the rotary table 200 (Installed).

4 and 5, the measurement cell 300 includes four first, second, third, and fourth measurement cells 300a and 300b spaced apart from each other to correspond to an angle of 90 ° with respect to the rotary table 200 The receiving grooves 310 are formed on the upper surface of each of the measurement cells 300 before being inverted. The receiving grooves 310 include four first, second, third and fourth receiving grooves 310a, 310b, 310c and 310d. Has a concave formed structure.

3, the rotating unit 700 includes a fixed block 710 provided on the upper side of the rotary table 200 and a fixed block 710 disposed inside the fixed block 710 and provided on one side of the measuring cell 300 A rotary motor 720 having a first rotary block 730 coupled to the rotary gear 725 to rotate in conjunction with the rotary gear 725 and a rotary gear 725 rotationally driven as external power is applied, And a fixing bracket 750 provided above the rotary table 200 so that the second rotary block 740 provided at the other side of the rotary table 200 is connected to the connection pin 760.

A plurality of receiving grooves 310 are formed on the upper surface of the measurement cell 300 so as to contain the algal culture fluid and the analysis sample. A plurality of receiving grooves 310 are formed on the upper surface of the measuring cell 300, And a second rotation block 740 having a groove into which a part of the connection pin 760 is inserted corresponding to the fixing bracket 750 is formed on the other side of the upper surface.

One end of the connecting pin 760 is accommodated in the fixing bracket 750 and the other end is accommodated in the second rotating block 740 to rotate the fixing bracket 750 and the second rotating block 740 Possibly pin-connected.

Accordingly, the plurality of measurement cells 300 can be rotated by 180 ° so as to be reversed with respect to the rotary table 200. However, after measuring the toxicity change value through the digital camera device 500, The analysis number is rotated so as to be inverted only in the cleaning process corresponding to the fourth measurement position before being injected into the receiving groove 310 of the measurement cell 300. [

The purified water tank 10 has a first inlet through which external purified water is introduced and a first outlet through which purified water is discharged. The sample tank 30 is connected to a second inlet through which sample analysis water is introduced from the outside, A second outlet is provided.

In addition, the water retention tank 40 is further provided with a cleaning nozzle 45 installed therein for spraying purified water for cleaning toward the inverted measurement cell 300.

The water retention tank 40 has a third inlet for supplying purified water for cleaning to the cleaning nozzle 45 and a drain for draining the algal culture liquid and sample analysis water to the outside.

6 and 7, the syringe injector 400 includes two syringe needles 410 and a syringe body 420, and the syringe needle 400 and the syringe needle 410 are connected to the syringe needle 410, During the lifting and lowering operation, the purified water, the algal culture fluid, and the sample analysis water are respectively stored in the syringe body 420 and then discharged to the outside of the syringe body 420.

The syringe body 420 has a cylindrical shape and a graduated scale on the outer peripheral surface thereof.

The syringe body 400 is filled with purified water and stored in the syringe body 420 so that the inside of the syringe body 420 is cleaned and the syringe body 420 is cleaned with purified water. The sample analysis numbers are temporarily stored and then injected into the receiving grooves 310 of the measuring cell 300, respectively.

8 and 9, the moving unit 800 includes a guide member 810 installed in the upper hollow portion 110 of the main body 100 in the transverse direction, and a connection bracket 820 And is supported by a first screw shaft 835 and a guide rod 836 and is connected to a connection block 840 which is moved left and right along the guide member 810 when the first screw shaft 835 rotates And a first screw shaft 835 axially coupled to the connection block 840. The first and second screw shafts 835 and 835 are driven in forward and reverse directions as external power is applied to move the connection block 840 in the left and right directions A first moving block 845 supported by the connecting bracket 820 and a second moving block 840 supported by the first moving block 845 and rotated in forward and reverse directions as external power is applied A second moving motor 850 having a second screw shaft 855 and a second moving motor 850 axially coupled to the second screw shaft 855, A lifting block 860 supported by the first guide bar 856 and operated to be lifted and lowered when the second screw shaft 855 is driven to rotate and a second moving block 860 coupled to the lifting block 860, A third moving motor 880 supported by the second moving block 870 and having a third screw shaft 885 driven in forward and reverse directions as external power is applied; Is supported by the second guide bar 886 which is axially coupled to the shaft 885 and is supported by the second moving block 870 and is operated to move up and down when the third screw shaft 885 is driven to rotate, And a lifting plate (890) connected to the piston (430) of the syringe injector (400) in an interlocking manner.

The first, second and third moving motors 830, 850, and 880 may employ a servo motor.

The syringe body 420 of the syringe injector 400 has a structure fixed to a lower portion of the second moving block 870.

The first moving motor 830 moves the connecting block 840 in the left and right directions and the second moving motor 850 moves the elevating block 860 upward and downward, 400 can move left, right and up and down in the X and Y axis directions.

The third moving motor 880 moves the piston 430 of the syringe injector 400 connected to the lifting plate 890 upward and downward by lifting the lifting plate 890 upward and downward through the connecting member 895, The syringe needle 410 connected to the piston 430 is lifted and lowered to suck and discharge the purified water, the sample analysis water, and the algal culture liquid into the syringe body 420.

The digital camera apparatus 500 is disposed in the upper hollow portion 110 and is disposed on one of the plurality of measurement cells 300 rotated by the rotary table 200 in a rotary manner, Up and down.

The digital camera apparatus 500 is provided with an optical image sensor 510 and a light mechanism 520. The light mechanism 520 includes a measuring light, an actinic light, and a photosynthesis induction light pulse light), and parameters such as Fv / Fm (maximum quantum yield) and ETRmax (maximum electron transfer efficiency) through the fluorescence reaction of chlorophyll a reaction center are used.

The measuring light irradiates weak light which is not used for photosynthesis among the light energy absorbed by the chlorophyll and induces it to emit fluorescence, and the actinic light causes photosynthesis.

6, the elevating means 900 includes a guide block 910 vertically installed on the upper hollow portion 110, a guide block 910 connected to the elevating motor 920 so as to be vertically guided, And a lifting body 930 having a ccd camera mounted on the lower part thereof.

The guide block 910 may employ an LM guide.

The culture tank 20 is provided with a pump for oxygen supply and circulation for culturing algae, and an LED light source is provided.

The purifier water tank 10, the sample tank 30, and the culture tank 20 are open at the upper part to allow the syringe needle 410 to enter and exit, and an entrance for entering and exiting the syringe needle 410 is formed at the upper corner part. do.

The present invention having such a configuration is characterized in that the measurement cell 300 in which the alga culture fluid and the sample analysis water are injected into the receiving groove 310 so as to divide the plurality of measurement cells 300 in a state where the rotary table 200 is stopped The measurement cell 300 which is called the first measurement cell 300a and is located in the rotary rotation direction from the first measurement cell 300a is referred to as a second measurement cell 300b, The measuring cell 300 located at the lower side is referred to as a third measuring cell 300c and the measuring cell 300c positioned in the rotary direction of rotation from the third measuring cell 300c and inverted by the rotating means 700 When the syringe needle 410 is moved downward by the moving means 800 after the syringe injector 400 is lowered by the moving means 800 as shown in FIGS. 10 and 11, 3 moving motor 880, the descending operation and the ascending operation are repeated a plurality of times, To the inlet and outlet in the body 420, thereby performing a first washing step (S1) to wash the syringe body 420.

Subsequently, after the syringe injector 400 is moved upward and laterally (rightward in FIG. 10) by the moving means 800, after the first moving step S2, the syringe injector 400 is lowered And a sample analyzing water sucking step (S3) for sucking sample analyzing water from the sample tank (30) by a descending and ascending operation of the syringe needle (410) and temporarily storing the sample analyzing water in the syringe body (420).

A second moving step S4 of moving the syringe injector 400 to the upper side of the first measuring cell 300a and moving the syringe injector 400 laterally by the moving means 800, The syringe needle 410 is lowered by the third moving motor 880 and the sample analyzing water in the syringe body 420 is moved to the receiving groove 310 of the first measuring cell 300a, A sample analyzing step in which a part of the sample is put into the first and second receiving grooves 310a and 310b and the rest is introduced into the third and fourth receiving grooves 310c and 310d among the receiving grooves 310 of the first measuring cell 300a (S5).

At this time, when the sample analysis number contained in the syringe body 420 is 4 cc, 2 cc is injected into the first and second receiving grooves 310a and 310b and 2 cc is injected into the third and fourth receiving grooves 310c and 310d .

After the syringe injector 400 is moved upward and laterally to return to the upper side of the purified water tank 10, the syringe injector 400 is lowered and the syringe needle 410 is lowered and raised. (S6) for sucking, discharging and discharging the gas into the second chamber (420).

A third moving step (S7) for raising the sludge injector (400) again after the second washing step and moving the raised syringe injector (400) to the upper side of the culture tank (20) After the moving step, the algae culture liquid sucking step (S8) for lowering the syringe injector (400) and sucking the algal culture liquid in the culture tank (20) by the descending and ascending operation of the syringe needle (410) ).

A fourth moving step (S9) of raising the syringe injector (400) and moving the syringe injector (400) to the upper side of the first measuring cell (300a) by using the moving means (800) A part of the algae culture liquid is introduced into the first and second receiving grooves 310a and 310b of the first measurement cell 300a in which the sample analyzer is contained by the lowering operation of the syringe needle 400, (Step S10), in which the remaining algae culture liquid is introduced into the third and fourth receiving grooves 310c and 310d of the first measurement cell 300a.

At this time, when the alga culture fluid contained in the syringe body 420 is 4 cc, 2 cc is injected into the first and second receiving grooves 310a and 310b and 2 cc is injected into the third and fourth receiving grooves 310c and 310d .

Thereafter, the syringe body 400 is lifted and moved in the lateral direction to return to the upper side of the purified water tank 10, and then the purified water is sucked and discharged into the syringe body 420, The third washing step S11 is performed.

The step of injecting the sample analysis water and the algae culture liquid into the measurement cell 300 at the first measurement position using the syringe injector 400 is repeatedly performed in the order described above.

Thereafter, the rotary table 200 is rotated by an angle of 90 ° using the driving means 600 to rotate the first measurement cell 300a to the second measurement position where the second measurement cell in the stopped state is located, The second measurement cell 300b is rotated to the third measurement position below the digital camera apparatus 500 and the third measurement cell 300c is rotated to the fourth measurement position where the fourth measurement cell in the stopped state is located do.

The first measurement cell 300a charged into the first, second, third, and fourth receiving grooves 310a, 310b, 310c, and 310d is placed in the second measurement position where the stopped second measurement cell is located, While waiting for the toxic change occurring in the mixed state of the algal culture solution and the sample analysis water to occur.

At this time, as the second measurement cell 300b is located at the third measurement position (lower side of the digital camera device 500) during the rotation of the rotary, the elevation means 900 is operated to descend to mix the algae culture liquid and the sample analysis water The light of the digital camera device 500 is stored in the second measurement cell 300b so that the chlorophyll reaction occurs on the first, second, third and fourth receiving grooves 310a, 310b, 310c and 310d. The fluorescence reaction generated by irradiation of the water can be used to intuitively measure the toxicity change value with the optical image sensor 510 and can be monitored in real time using the image output to the monitor.

The third measurement cell 300c is rotated 180 degrees by the rotating means 700 at the fourth measurement position during the rotation of the rotary, and the first, second, third, and fourth receiving grooves 310a, 310b, 310c, 310d are poured into the water collecting tank 40. [0051] As shown in FIG.

Thereafter, the cleaning water is jetted from the cleaning nozzle 45 to the first, second, third, and fourth receiving grooves 310a, 310b, 310c, and 310d of the third measurement cell 300c in an inverted state, 310, 310c, and 310d, and the cleaned purified water for cleaning is contained in the water retention tank 40 and then drained to the outside through the drain port.

The fourth measurement cell 300d is rotated 180 degrees again in the inverted state to rotate the first, second, third, and fourth receiving grooves 310a, 310b, 310c, and 310d upward, After the alga culture liquid and sample analyzing water are again returned to the first measurement position where the first measurement cell of the fourth measurement cell 300d is located, 310b, 310c, and 310d, and the above-described processes are repeated so that continuous measurement tests can be performed.

Accordingly, the present invention relates to a digital camera device (hereinafter, referred to as " digital camera device ") equipped with an optical image sensor 510 capable of imaging a toxic change value while irradiating light to induce chlorophyll fluorescence reaction in a state where sample analysis water supplied from outside is mixed with an algal culture liquid 500), it is possible not only to monitor the toxicity change value on the monitor in real time intuitively but also to continuously perform the measurement test automatically by using the rotation table 200, Respectively.

The rotating means 700 of the present invention can rotate the measuring cell 300 by 180 ° with the driving torque of the rotating motor 720 so that the algal culture liquid and the sample analyzing water contained in the receiving groove 310 are discharged It can be easily poured into the tank 40 and the inside of the receiving groove 310 of the inverted measurement cell 300 can be easily cleaned by using the cleaning nozzle 45.

The rotating table 200 according to the present invention is configured such that the four measuring cells 300 are rotated at an angle of 90 ° by the driving means 600 so that the cleaning of the syringe body 420, The mixing of the analysis water and the algal culture liquid, the measurement of the digital camera device 500, and the cleaning process of the measurement cell 300 can be performed successively.

The moving means 800 of the present invention can move up and down the syringe injector 400 in the upward and downward directions and in the left and right directions as well as move the syringe needle 410 up and down using the third moving motor 880 The cleaning operation of the syringe body 420 using purified water and the quantification of the algal culture liquid and the sample analysis water can be carried out in the first, second, third and fourth receiving grooves 310a, 310b, 310c and 310d. .

Although the present invention has been described in detail with reference to specific embodiments, it is to be understood that the invention is not limited to the disclosed embodiments. Therefore, the scope of the present invention should not be limited to the above-described embodiments, but should be determined by the appended claims, as well as the appended claims.

That is, the present invention described above is not limited to the above-described specific preferred embodiments, and any person skilled in the art will be allowed to use the present invention without departing from the gist of the present invention. It will be understood that various modifications can be made without departing from the spirit and scope of the invention as defined in the appended claims.

10: Purified water tank 20: Culture tank
30: sample tank 40:
45: Cleaning nozzle 100: Body
110: upper hollow portion 120: lower space portion
150: check port 200: rotating table
210: through hole 300: measuring cell
300a, 300b, 300c, 300d: first, second, third,
400: syringe injector 410: syringe needle
420: syringe body 430: piston
500: Digital camera device 510: Optical image sensor
520: light mechanism 600: driving means
610: Driving motor 615: Driving pulley
620: rotating shaft 625: driven pulley
700: rotating means 710: fixed block
720: rotation motor 725: rotary gear
730: first rotating block 740: second rotating block
750: Fixing bracket 760: Connection pin
800: moving means 810: guide member
820: connection bracket 830: first moving motor
835: first screw shaft 836: guide rod
840: connection block 845: first movement block
850: second moving motor 855: second screw shaft
856: first guide bar 860: lift block
870: second moving block 880: third moving motor
890: Steel plate 895: Connecting member
900: elevating means 910: guide block
920: lifting motor 930: lifting body

Claims (6)

The inner space is divided into the upper space 110 and the lower space 120 with reference to the rotary table 200 and the purified water tank 10 and the sample tank 30 A main body 100 provided with a culture tank 20 and a water retention tank 40 containing an algal culture liquid;
The lower end of the syringe injector 400 is connected to the upper end of the lower space 120 by a driving unit 600 at a predetermined angle, A rotation table 200 having a plurality of through holes 210 formed therein so as to be lowered;
And is arranged to be rotatable by 180 ° so as to be turned upside down by the rotating means 700 and arranged to perform different processes at every rotation of the rotary table 200 at a predetermined angle, A plurality of measurement cells (300) having a groove (310);
The syringe needle 410 is installed in the upper hollow portion 110 and is moved leftward, rightward and upward and downward by the moving means 800 so that the syringe needle 410 can be elevated upward and downward. The purified water tank 10 and the sample tank A syringe injector 400 injecting the sample analysis water and the algal culture liquid into any one of the plurality of measurement cells 300, the syringe injector 400 being capable of injecting the contents contained in the culture tank 20 and the culture tank 20 into the respective measurement cells 300; And
And an algae culture liquid placed in one of the plurality of measurement cells 300 at the time of the lowering operation is disposed in the upper hollow portion 110 above the rotary table 200 and is vertically movable up and down by the elevating means 900, And a digital camera device (500) for measuring a change in toxicity of the analyzed sample water with the optical image sensor (510) and outputting the measured value to a monitor,
The rotating unit 700 includes a fixed block 710 provided on the rotating table 200 and a fixed block 710 mounted on the fixed block 710 and having one side of the measuring cell 300 interlocked with the rotating gear 725, A rotation motor 720 having a rotation gear 725 which is rotated as the external power source is applied and the other side of the measurement cell 300 is connected to the connection pin 760, And a fixing bracket 750 provided on the fixing bracket 750,
The elevating means 900 includes a guide block 910 installed vertically to the upper hollow portion 110 and a lift motor 920 guided and guided by the guide block 910 so as to be able to move up and down. Water quality monitoring system using algae. The method according to claim 1,
The driving unit 600 includes a driving motor 610 installed in the lower space 120 and provided with a driving pulley 615,
And a rotary shaft 620 provided at a lower portion of the rotary table 200 and having a driven pulley 625 connected to the drive pulley 615 by a belt and rotated in conjunction therewith to transmit rotational force to the rotary table 200 A water quality monitoring system using microalgae. The method according to claim 1,
The syringe injector 400 is composed of at least two syringe injectors 400 that operate simultaneously,
The syringe injector 400 is configured to perform an operation of cleaning the syringe body 420 by sucking and discharging purified water into the syringe body 420 when the sample analysis water or algae culture liquid is introduced into the receiving groove 310 Water quality monitoring device using microalgae. The method according to claim 1,
Further comprising a cleaning nozzle (45) installed in the water retention tank (40) and injecting cleaning purified water toward the inverted measurement cell (300). The method according to claim 1,
The rotary table 200 is rotated at an angle of 90 degrees by the driving means,
Wherein the measurement cells (300) are arranged at four positions on the rotary table (200) so as to be rotated at angles of 90 占 and perform respective processes. The method according to claim 1,
The moving means 800 includes a guide member 810 installed in the transverse direction in the upper hollow portion 110 of the main body 100,
The first screw shaft 835 is connected to the guide member 810 by a connection bracket 820 and is supported by a first screw shaft 835 and a guide rod 836. When the first screw shaft 835 rotates, A connection block 840 which is moved left and right,
The connection block 840 has a first screw shaft 835 which is axially coupled to the connection block 840 and is driven in forward and reverse directions as external power is applied to move the connection block 840 in the left and right directions. 830,
A first moving block 845 supported by the connection bracket 820,
A second moving motor 850 supported by the first moving block 845 and having a second screw shaft 855 driven in forward and reverse directions as external power is applied,
A first guide bar 856 which is axially coupled to the second screw shaft 855 and is supported by the first moving block 845 so as to be moved up and down during rotation of the second screw shaft 855, (860)
A second moving block 870 coupled to the lift block 860 and coupled to the syringe body 420 of the syringe injector 400 to be fixed thereto,
A third moving motor 880 supported by the second moving block 870 and having a third screw shaft 885 driven in forward and reverse directions as external power is applied,
Is supported by a second guide bar 886 which is axially coupled to the third screw shaft 885 and is supported by the second moving block 870 so that the third screw shaft 885 is moved up and down when the third screw shaft 885 is driven to rotate, And a lifting plate (890) connected to the piston (430) of the syringe injector (400) through the passage (895).

Images