KR20150089402A - Measuring apparatus of water toxicity using algae and method thereof - Google Patents
Measuring apparatus of water toxicity using algae and method thereof Download PDFInfo
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- KR20150089402A KR20150089402A KR1020140009963A KR20140009963A KR20150089402A KR 20150089402 A KR20150089402 A KR 20150089402A KR 1020140009963 A KR1020140009963 A KR 1020140009963A KR 20140009963 A KR20140009963 A KR 20140009963A KR 20150089402 A KR20150089402 A KR 20150089402A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
- G01N33/1866—Water using one or more living organisms, e.g. a fish using microorganisms
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Abstract
Description
The present invention relates to an apparatus and method for measuring water toxicity using algae, and more particularly, to a method and apparatus for measuring the toxicity of a water by using a three-color light filter of a blue filter, a red filter and a yellow filter The present invention relates to an apparatus for measuring water toxicity using algae and a method for measuring ecotoxicity such as test water (effluent from a river water or a water treatment facility) by digitizing the difference in chromaticity.
Recently, water pollution has become a problem as an environmental problem. Thus, there is a growing interest in methods for measuring the toxicity of water. The device for measuring the toxicity of water quality has been developed by many researchers, but the development of the chemical water quality toxicity measuring device has been mainly made. The chemical water quality toxicity measuring device can be quantitatively measured only in a substance known to the water system, and it is disadvantageous in that it can be used only by a high-priced installation cost and a skilled technical researcher. In recent years, development of a biological water quality toxicity measuring device has been developed to compensate for such problems, and devices for measuring water quality toxicity have been developed using fish, daphnia, luminescent bacteria, microalgae and microbial cells.
The water quality toxicity measuring device using fish is a device for measuring the toxicity of water quality by using the reflux property of fish which goes up the water flow. However, the water quality toxicity measuring device using fish has a disadvantage in that the sensitivity of the toxicity detecting object is large and the measurement time and error range are large.
When the water is not contaminated by toxic substances, the water daphnia shows regular movement and when the toxic substances are introduced, the movement of water daphnia changes irregularly and decreases rapidly. Respectively. However, since the size of the daphnia is smaller than that of the fish, the sensitivity is better than that of the fish, but the culture of the daphnia must be changed 2-3 times a week, and it is troublesome to separate the mother and the baby every day.
The water quality toxicity measuring system using luminescent microorganisms measures the toxicity of water quality by measuring the luminescence of microorganisms responding to toxicity. However, in the case of a device using a luminescent microorganism, a light measuring device for measuring the luminescence amount is separately required. Since most of the luminescent microorganisms depend on imports, they are expensive in terms of cost, and have a disadvantage that specialized manpower for maintenance and management is required.
To solve these problems, Korean Patent Laid-Open No. 10-2012-0041096 has been proposed.
The prior patent discloses a case for forming a culture chamber for culture of control group parasites cultured in a water quality not containing toxicity and experimental group parasites cultured in a toxic measurement target water quality; A light source for irradiating light to the control group and the test group; A temperature regulator for regulating a temperature inside the culture chamber; A camera for photographing and imaging the parasites cultured in the culture room; And a second area ratio, which is a ratio of the total area of the control group and the area where the hue is changed to white, and the area ratio of the entire area of the test group to the white area, And calculating a toxicity of the toxic measurement target water quality by using the first area ratio and the second area ratio. Thus, not only can the toxicity of the water quality be measured, but also the speed and accuracy of the toxicity measurement of the water quality Can be secured.
However, the prior patent has a problem in that it is necessary to cultivate the palaea in the water to be measured which is adjusted to the concentrations of 100%, 50%, 25%, 12.5%, and 6.25% by the half-dilution method.
Accordingly, an object of the present invention is to solve the above-mentioned problems, and it is an object of the present invention to provide a pharmaceutical composition containing chlorpin-a, b, c, d and β-carotene, phycobilin, By measuring the change in the chromaticity of cells that change when a coloring matter such as fucoxanthin is exposed to a toxic substance using a three-color light filter of a blue filter, a red filter and a yellow filter, the toxicity of the effluent of the river water or water treatment facility And to provide a method and apparatus for measuring water toxicity using algae that can be monitored.
The apparatus for measuring water toxicity using algae according to the present invention comprises: a solution storage part having a culture solution container for culturing a culture solution necessary for culturing algae and a sample container for storing drainage water of a river water or water treatment facility; A filter unit connected to the solution storage unit and filtering the suspended substances before transferring the water from the river water or the water treatment facility to the reserve tank; A solution transferring part connected to the filter part and supplying a culture solution from the solution storing part to the reservoir tank and the photochemical sensor part and supplying drainage water from the filter part to the reserve tank and the photochemical sensor part, Wow; A photochemical sensor unit connected to the solution transfer unit and the central processing unit and equipped with a photochemical sensor capable of measuring a light quantity of a wavelength band of a visible light region and amplifying and digitizing an analog value of the measured light quantity; A signal communication unit connected to the central processing unit, for transmitting data digitized by the photochemical sensor unit to the terminal of the control center via wire / wireless, and for transmitting the command transmitted from the terminal of the control center to the central processing unit; A display unit connected to the central processing unit and displaying a difference between an analog light amount value measured from an algae exposed to the algae on the culture liquid and pollutants, an amplified digital value and a difference between the two values in real time in a graphical form; And a central processing unit for operating an operating system (OS) connected to the photochemical sensor unit, the signal communication unit, the temperature control unit, and the display unit, and controlling a change in chromaticity of the cells, And measuring the change in the chromaticity by using the photochemical sensor unit, and calculating the water toxicity using the data change in chromaticity.
The method for measuring toxicity of water using algae according to the present invention comprises the steps of sucking the discharge water of a river water or a water treatment facility into a sample container of a solution storage part or sucking a culture solution into a culture solution container of the solution storage part; A step of delivering the discharge water of the river water or the water treatment facility to the sample container of the solution storage part or delivering the culture solution to the culture solution container of the solution storage part; The diluted culture liquid is delivered to the sample container of the solution storage portion; The algae being supplied to the sample container and the culture liquid container of the solution storage portion; The sample to which the alga is supplied is measured by a photochemical sensor, and the culture liquid supplied with the algae is measured by a photochemical sensor for checking with the algae-fed sample; The data on the sample supplied with the algae measured by the photochemical sensor and the data on the culture fluid supplied with the algae measured by the photochemical sensor are transmitted to the central processing unit; And analyzing and converting the transmitted data in the central processing unit; And outputting result values and graphs on the screen using the analyzed and transformed data; Wherein the sample to which the alga is supplied and the culture solution to which the algae are supplied are measured with a photochemical sensor to compare the change in the color of the algae cells caused by the change in pigment in the algae cells to thereby calculate the water toxicity.
As described above, the apparatus and method for measuring water quality toxicity using algae according to the present invention are characterized in that chromaticity changes of cells that change when a bird is exposed to a toxic substance are detected using a three-color light filter of a blue filter, a red filter and a yellow filter It is advantageous in that the maintenance cost is low and the cultivation is easy compared with the water quality toxicity measuring device using other species to measure water quality toxicity by measuring the data of the change in chromaticity and using the data change in chromaticity change.
In addition, there is an advantage in that it is very advantageous in automation because of a long biological supply interval for maintenance and easy management of the apparatus.
In addition, it has the advantage of reducing the damage caused by water pollution and monitoring river ecology more precisely from water pollution sources, thereby protecting the river ecosystem and water resources, and further enhancing public health.
1 is a schematic block diagram of an apparatus for measuring water quality toxicity using algae according to the present invention.
Figs. 2A to 2G are photographs of the main part of Fig.
3 is a perspective view of an apparatus for measuring water quality toxicity using algae according to the present invention.
4A-4C are a plan view, a front view and a side view of Fig. 2;
FIG. 5 is a flow chart for explaining a method for measuring water toxicity using algae according to the present invention. FIG.
6A and 6B are graphs and graphs of water toxicity measurements using algae according to the present invention.
FIGS. 7A to 7C are SEM diagrams showing the measurement of the algae before and after exposure of toxic substances using a three-color light filter of a blue filter, a red filter, and a yellow filter. FIG.
FIG. 8 is a graph showing the resultant values of FIG. 6 and FIG. 7, and the ecotoxicity value converted using the SEM.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, an apparatus and method for measuring water toxicity using algae according to the present invention will be described in detail with reference to the accompanying drawings. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. The following terms are defined in consideration of the functions of the present invention, and they may vary depending on the intentions or customs of the client, the operator, the user, and the like. Therefore, the definition should be based on the contents throughout this specification.
Like numbers refer to like elements throughout the drawings.
FIG. 1 is a schematic block diagram of an apparatus for measuring a water toxicity using algae according to the present invention, FIGS. 2A to 2G are photographs of the main part of FIG. 1, and FIG. FIG. 5 is a flow chart for explaining a method for measuring water toxicity using algae according to the present invention, and FIGS. 6A and 6B are views FIGS. 7A to 7C are graphs showing the results and graphs of the water quality toxicity measurement using the algae according to the present invention. FIGS. 7A to 7C are SEM images And FIG. 8 is the ecotoxicity value converted using the results and graphs of FIGS. 6A and 6B and FIGS. 7A to 7C and the SEM.
1 to 4C, the apparatus for measuring water toxicity using algae according to the present invention includes a
Here, the
The
The
The
The
The
The
Hereinafter, a method for measuring water toxicity using algae according to the present invention will be described in detail with reference to FIG.
First, the discharge water of the river water or the water treatment facility is sucked into the sample container of the solution storage part 110 (S510), or the culture solution is sucked into the culture solution container of the solution storage part 110 (S510-1). Here, the order of the inhalation of the effluent from the river water or the water treatment facility or the inhalation of the culture fluid first is irrelevant. As described above, the culture solution in the
Thereafter, the discharge water of the river water or the water treatment facility is transferred to the sample container of the solution storage part 110 (S520), or the culture solution is transferred to the culture solution container of the solution storage part 110 (S520-1). At this time, steps S520 and S520-1 are performed at the same time. If the culture solution is stored for culturing the algae in the solution container of the
Thereafter, the diluted culture liquid is transferred to the sample container of the solution storage unit 110 (S530).
Thereafter, the algae are supplied to the sample container and the culture container of the solution storage unit 110 (S540).
Thereafter, the sample supplied with the algae is measured with a photochemical sensor (S550), and the culture liquid supplied with the algae is measured by the photochemical sensor for comparison with the algae-fed sample (S550-1). At this time, steps S550 and S550-1 are performed simultaneously.
Thereafter, the data of the sample supplied with the algae measured by the photochemical sensor and the data of the culture fluid supplied with the algae measured by the photochemical sensor are transmitted to the central processing unit 180 (S560).
Thereafter, the
Thereafter, the resultant value and the graph are displayed on the screen using the analyzed and converted data (S580).
The result values and graphs thus displayed on the screen are as shown in FIGS. 6A and 6B.
Meanwhile, the chromaticity change of the cell due to the change of the pigment in the bird cell before the algae exposed to the toxic substance and after the algae exposed to the toxic substance measured in the steps S550 and S550-1 is referred to as a blue filter, a red filter, The SEM measured using the three-color light filter is as shown in Figs. 7A to 7C. Herein, the pigment in the bird cell before the algae is exposed to the toxic substance is as shown on the left, and the dye in the bird cell after the algae is exposed to the toxic substance is as shown on the right. 7A shows a case where a blue light filter is applied, FIG. 7B shows a case where a red light filter is applied, and FIG. 7C shows a case where a yellow light filter is used.
The ecotoxicity values converted by using the results and graphs shown in FIGS. 6A and 6B and FIGS. 7A to 7C and the SEM are numerically expressed. The ecotoxicity values thus quantified are as shown in FIG.
As described above, the apparatus and method for measuring water quality toxicity using algae according to the present invention are characterized in that chromaticity changes of cells that change when a bird is exposed to a toxic substance are measured using a three-color light filter of a blue filter, a red filter and a yellow filter The data of the measured chromaticity are converted into data and the data of the chromaticity change is used to measure the water toxicity, the maintenance cost is lower and the cultivation is easier than the water quality toxicity measuring device using other species. In addition, it is very advantageous for automation because it has a long interval of biological supply for maintenance and is easy to manage the apparatus. In addition, it can reduce the damage caused by water pollution and monitor river ecology more precisely from water pollution source, thereby protecting river ecosystem and water resources, and further increasing national health.
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, Various changes, modifications or adjustments to the example will be possible. Therefore, the scope of protection of the present invention should be construed as including all changes, modifications, and adjustments that fall within the spirit of the technical idea of the present invention.
110: solution storing part 120: filter part
130: solution transfer part 140: photochemical sensor part
150: signal communication unit 160: temperature control unit
170: display unit 180: central processing unit
Claims (5)
A filter unit 120 connected to the solution storage unit 110 and filtering out suspended solids before transferring the effluent of the water or water treatment facility to the reserve tank;
And supplies the culture liquid from the solution storage unit 110 to the reservoir tank and the photochemical sensor unit 140. The filter unit 120 is connected to the reservoir tank and the photochemical sensor unit A solution transfer unit 130 for supplying the drainage of the river water or the water treatment facility to the water supply unit 140;
A photochemical sensor unit connected to the solution transfer unit 130 and the central processing unit 180 and equipped with a photochemical sensor capable of measuring the amount of light in the wavelength region of the visible light region and amplifying and digitizing an analog value of the measured light amount 140;
The control unit 180 is connected to the central processing unit 180. The photochemical sensor unit 140 transmits the digitized data to the terminal of the control center via wire or wireless communication and transmits the command transmitted from the terminal of the control center to the central processing unit 180. [ A signal communication unit 150 for transmitting the signal to the mobile station;
A display unit 170 connected to the central processing unit 180 and displaying the difference between the analog light amount measured from algae exposed to the pollutants on the culture liquid and the amplified digital value and the two values in real time in a graph, And;
And a central processing unit 180 connected to the photochemical sensor unit 140, the signal communication unit 150, the temperature control unit 160 and the display unit 170 and operating an OS (Operating System) A change in chromaticity of a cell that changes when a bird is exposed to a toxic substance is measured using the photochemical sensor unit 140, data of the measured chromaticity change is calculated, and water quality toxicity is calculated using the data change in chromaticity Water toxicity measuring device using algae.
The solution storage part 110 stores the culture solution for 10 to 15 days, stores distilled water for cleaning the intermediate reserve tank and the measuring acrylic tube for 10 to 15 days,
Wherein the photochemical sensor unit 140 includes a red light (590 to 610 nm) filter, a yellow light (500 to 700 nm) filter, and a blue light (400 to 450 nm) filter. .
The central processing unit 180 calculates the total light amount of each wavelength band by dividing the light amount measured from the photochemical sensor unit 140 by the range of the red light, the yellow light, and the blue light, And comparing the light quantity of the algae exposed to the light source with the light quantity of the algae exposed to the light source.
(S520-1) of delivering the culture solution to the culture solution container of the solution storage part 110 (S520-1);
(S530) of delivering the diluted culture solution to the sample container of the solution storage unit 110;
A step S540 of supplying the alga to the sample vessel and the culture vessel of the solution storage unit 110;
A step S550-1 of measuring the sample supplied with the algae with the photochemical sensor (S550), measuring the culture solution supplied with the algae with the photochemical sensor for comparison with the algae-fed sample;
A step (S560) of transmitting data on the sample supplied with the algae measured by the photochemical sensor and data on the culture solution supplied with the algae measured by the photochemical sensor to the central processing unit 180;
(S570) of analyzing and converting the transmitted data in the central processing unit (180);
And outputting a result and a graph on the screen using the analyzed and converted data (S580);
Characterized in that the sample to which the algae are supplied and the culture liquid to which the algae are supplied are measured with a photochemical sensor to compare the change in chromaticity of the algae cells due to the change in pigment in the algae cells to determine the toxicity of the algae Methods for measuring water quality toxicity.
If the culture solution for culture of algae is stored in the culture solution container of the solution storage part 110, step S520-1 is not necessary;
Wherein the step S550 and the step S550-1 are performed simultaneously.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017159895A1 (en) * | 2016-03-17 | 2017-09-21 | 주식회사 켐토피아 | Water toxicity measurement apparatus using algae, and method therefor |
| KR101838729B1 (en) * | 2017-11-08 | 2018-03-14 | 한국해양과학기술원 | Eotoxicity evaluation analyzer device using microalgae image-analysis program and method thereof |
| KR101951330B1 (en) | 2018-03-27 | 2019-02-22 | 주식회사 켐토피아 | Device for monitoring quality of water |
| KR102156882B1 (en) | 2019-11-18 | 2020-09-16 | 주식회사 켐토피아 | Device for monitoring quality of water |
| KR102594012B1 (en) * | 2022-10-19 | 2023-10-26 | 동문이엔티(주) | Toxicity monitor using algae |
-
2014
- 2014-01-27 KR KR1020140009963A patent/KR20150089402A/en not_active Application Discontinuation
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017159895A1 (en) * | 2016-03-17 | 2017-09-21 | 주식회사 켐토피아 | Water toxicity measurement apparatus using algae, and method therefor |
| KR101838729B1 (en) * | 2017-11-08 | 2018-03-14 | 한국해양과학기술원 | Eotoxicity evaluation analyzer device using microalgae image-analysis program and method thereof |
| WO2019093741A1 (en) * | 2017-11-08 | 2019-05-16 | 한국해양과학기술원 | Ecotoxicity evaluation analyzing device including microalgae image analysis program installed therein, and method thereof |
| US11526981B2 (en) | 2017-11-08 | 2022-12-13 | Korea Institute Of Ocean Science & Technology | Ecotoxicity evaluation analyzing device including microalgae image analysis program installed therein, and method thereof |
| KR101951330B1 (en) | 2018-03-27 | 2019-02-22 | 주식회사 켐토피아 | Device for monitoring quality of water |
| KR102156882B1 (en) | 2019-11-18 | 2020-09-16 | 주식회사 켐토피아 | Device for monitoring quality of water |
| KR102594012B1 (en) * | 2022-10-19 | 2023-10-26 | 동문이엔티(주) | Toxicity monitor using algae |
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