KR101915074B1 - 토마토의 여교배 육종을 위한 단일염기다형성 마커 세트 및 이의 용도 - Google Patents

토마토의 여교배 육종을 위한 단일염기다형성 마커 세트 및 이의 용도 Download PDF

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KR101915074B1
KR101915074B1 KR1020170062466A KR20170062466A KR101915074B1 KR 101915074 B1 KR101915074 B1 KR 101915074B1 KR 1020170062466 A KR1020170062466 A KR 1020170062466A KR 20170062466 A KR20170062466 A KR 20170062466A KR 101915074 B1 KR101915074 B1 KR 101915074B1
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Abstract

본 발명은 SNP(single nucleotide polymorphism) 위치 염기를 포함하는 서열번호 1 내지 143의 염기서열로 이루어진 폴리뉴클레오티드로부터 선택되는 하나 이상의 폴리뉴클레오티드 또는 이의 상보적 폴리뉴클레오티드를 포함하는, 마커이용여교잡(MAB, marker assisted backcross) 육종을 위한 토마토의 유전적 배경 또는 품종 진단용 SNP 조성물에 관한 것으로, 본 발명의 SNP는 토마토 신품종 육성에 있어 유용한 분자마커로 이용될 수 있을 것으로 기대된다.

Description

토마토의 여교배 육종을 위한 단일염기다형성 마커 세트 및 이의 용도{Single nucleotide polymorphism marker set for marker-assisted breeding in tomato and uses thereof}
본 발명은 토마토의 여교배 육종을 위한 단일염기다형성 마커 세트 및 이의 용도에 관한 것이다.
차세대 염기서열 시퀀싱 (next generation sequencing, NGS)의 발달로 다양한 작물에서의 표준유전체 정보가 밝혀지고 대용량 염기서열 생산이 가속화되면서 유전체 기반의 분자육종이 육종산업에서 가지는 중요도는 더욱더 커지고 있다. 분자육종은 원하는 형질의 유무를 표현형의 관찰 없이 염기서열의 차이를 나타내는 분자마커(molecular marker)를 이용해 판별하는 기법을 비롯하여 크게 발전하고 있다(Edwards & Batley, 2010, Plant Biotechnology Journal. 8:2-9).
분자육종 과정은 개량하고자 하는 새로운 형질을 도입하는 과정과 회복친(recurrent)의 우수형질을 유지시키는 과정으로 나눌 수 있다. 분자마커 이용 여교잡(Marker-assisted Backcrossing, MABC)은 후자에 속하는 기술로 3개의 하위 단계를 포함하고 있다. 첫 번째로 목표 형질 또는 QTL(Quantitative trait locus)과 연관된 개체를 선발하기 위해 마커를 사용하는 '포어그라운드 선발(foreground selection)'이 있고, 두 번째로 목표 유전자와 연관된 마커 사이의 재조합이 일어난 여교잡 자손을 선발하는 '재조합 선발(recombinant selection)'이 있다. 마지막으로 본 발명의 목적이기도 한 '백그라운드 선발(background selection)'은 목표유전자와 연관되어 있지 않은 마커를 사용하여 목표 유전자 부위를 제외한 모든 부분이 회복친의 유전체 구성을 갖는 여교잡 자손을 선발하는 것이다.
MABC는 분자마커를 사용하여 유묘기에 여교잡 자손의 염색체 전체 조성을 확인함으로써 여교잡 자손이 회복친의 우수형질을 가진 유전체 구성으로 회복하는데 걸리는 시간을 단축시킬 수 있는 중요한 육종기법이다(Edwards & Batley, 2010, Plant Biotechnology Journal. 8:2-9). 기존의 여교잡 육종의 경우 6-7 세대 이상의 시간이 소요되지만 분자마커를 이용한 여교잡 선발 (MABC) 육종은 빠른 경우에는 여교잡 2세대에서부터 개체를 선발할 수 있기 때문에 작물 개량에 소요되는 노력과 비용을 절감하여 육종의 규모와 효율성을 증대시킬 수 있다. 벼를 비롯한 다양한 작물에서 MABC 육종기술을 적용한 사례들이 보고되고 있으나 아직까지 육종에 실질적인 도움이 되는 MABC 기술은 미흡한 실정이다.
MABC를 실제 육종 현장에 적용시키기 위해서는 몇 가지 조건이 필요하다. 우선 유전체 전체적(genome-wide)으로 분포하고 있는 분자마커를 확보해야 하며 교배 조합에 따라 적용 가능한 마커를 쉽게 확인할 수 있어야 한다. 또한 유전형분석(genotyping)에 사용되는 분자마커만을 선발하여 분석에 소요되는 비용을 줄이는 것이 중요하다.
MABC용 마커 제작에는 주로 단일염기서열다형성(Single nucleotide polymorphism, SNP)를 이용하는데 이는 단순반복서열(Simple sequence repeat)이나 삽입/결실(insertion/deletion, indel)과 같은 변이에 비하여 유전체 내 발생빈도가 높은 구조 변이의 하나이며 탐색에서 분석에 이르는 전 과정이 자동화될 수 있기 때문이다. 하지만 과도하게 많은 SNP는 실험에 많은 시간과 비용이 소모되기 때문에 활용도가 높은 SNP를 선발하는 것이 중요하다.
한국등록특허 제1677714호에서는 '토마토 흰가루병 저항성 또는 이병성 품종 선별용 분자마커 및 이를 이용한 토마토 흰가루병 저항성 또는 이병성 품종 선별방법'이 개시되어 있고, 한국공개특허 제2016-0038243호에서는 '고추의 여교배 육종을 위한 단일염기다형성 마커 세트 및 이의 용도'가 개시되어 있으나, 본 발명에서와 같이, '토마토의 여교배 육종을 위한 단일염기다형성 마커 세트 및 이의 용도'에 대해서는 밝혀진 바가 전혀 없다.
본 발명은 상기와 같은 요구에 의해 도출된 것으로서, 본 발명에서는 앞서 선발된 tag-SNP 27,680 좌를 바탕으로 하여 실제 토마토의 MABC 육종에 활용 가능한 마커 세트를 선발하는 것을 목적으로 하였다. 234개의 SRA(Sequence Read Archive) 토마토를 이용하여 염색체 내 일정 간격(5-10mb)으로 영역을 나눈 다음 PIC(Polymorphism Information Content) value가 높은 SNP를 우선으로 하여 총 143개의 MABC용 후보 SNP를 선발하였으며, 다양한 토마토 계통들의 가상의 교배조합을 통해 마커의 활용 가능성을 검증하였다. 또한 real-time PCR을 이용하여 18개의 SNP 마커를 Luna probe(unlabled probe)를 이용하여 실제 교배 집단에서 검정하여 분석 결과와 실제 실험 결과가 높은 일치율을 보이는 것을 확인하였으며 선발된 후보 SNP 마커 세트의 활용 가능성을 확인함으로써, 본 발명을 완성하였다.
상기 과제를 해결하기 위해, 본 발명은 SNP(single nucleotide polymorphism) 위치 염기를 포함하는 서열번호 1 내지 143의 염기서열로 이루어진 폴리뉴클레오티드로부터 선택되는 하나 이상의 폴리뉴클레오티드 또는 이의 상보적 폴리뉴클레오티드를 포함하는, 마커이용여교잡(MAB, marker assisted backcross) 육종을 위한 토마토의 유전적 배경 또는 품종 진단용 SNP 조성물을 제공한다.
또한, 본 발명은 상기 SNP 또는 이의 cDNA를 포함하는 마커이용여교잡 육종을 위한 토마토의 유전적 배경 또는 품종 진단용 마이크로어레이를 제공한다.
또한, 본 발명은 상기 마이크로어레이를 포함하는 마커이용여교잡 육종을 위한 토마토의 유전적 배경 또는 품종 진단용 키트를 제공한다.
또한, 본 발명은 토마토 시료에서 게놈 DNA를 분리하여 각 SNP 위치 염기인 다형성 부위의 유전자형을 결정하는 단계를 포함하는 마커이용여교잡 육종을 위한 토마토의 유전적 배경 또는 품종 진단 방법을 제공한다.
본 발명에서 선발한 토마토 MAB 마커 세트는 단순히 단일 염기서열 치환(SNP)에 의해 발생된 변이를 임의로 선발하여 사용하던 기존의 방법에 비하여 정보력이 높은 테그 에스엔피(tag-SNP) 27,539개의 마커를 기반으로 선발하였으며 각 교배 집단들의 유전체 조성을 좀 더 효율적으로 대변할 수 있다. 또한 여교잡 육종현장에서 활용될 수 있는 가상의 양친을 대상으로 tag-SNP set의 적용성을 검증함으로서 다양한 여교잡 교배조합에 공통적으로 적용할 수 있는 가능성을 높였다.
본 발명에서 확보한 여교배선발을 위한 단일염기다형성(SNP) 마커 세트는 여교배 후 분리집단의 개체들이 가지고 있는 반복친과 회복친의 유전적 배경을 확인하기 위해 소요되는 시간과 노력을 획기적으로 단축할 수 있어 토마토 품종 육성에 있어서 경쟁력을 크게 향상시킬 수 있으므로 상업적으로 매우 유용하게 이용될 수 있을 것으로 기대된다.
도 1은 야생종과 재배종 토마토 간 SNP를 이용하여 확인한 유전체 내 관계와 과실 형태의 차이를 나타낸다(Tao Lin et al. 2014 Nature Genetics 46, 1220-1226).
도 2는 본 발명에서 최종 선발된 143개 마커의 염색체 상의 위치를 나타낸다. 12개의 토마토 염색체를 막대로 나타내었으며, 각 막대의 아래에 염색체의 길이(Mb)를 표시하였다. 왼쪽은 염색체의 영역을 나타냈으며 오른쪽에는 선발된 MABC 마커의 이름과 위치를 표시하였다.
도 3은 본 발명에서 선발된 MABC 1개 마커를 이용하여 토마토 124개 개체에서의 다형성을 조사한 결과를 나타낸다. 그림의 가로축과 세로축에는 124개의 개체를 순서대로 나열하였으며 각 개체별로 교배조합을 만들었을 때 선발된 143개의 마커에서 몇 개의 마커가 다형성을 나타내는지를 표시하였다. 다형성을 나타내는 마커의 개수가 많을수록 박스의 색이 진하게 나타나도록 설정하였다.
도 4는 본 발명의 124개 토마토 계통 내에서 19개의 대표 품종을 선발하여 교배조합별 다형성을 검증하였다. 19개 계통은 솔라눔 리코퍼시쿰(Solanum lycopersicum) (Group=BIG) 14개의 계통과 리코퍼시쿰 변종 세라시포르메(Solanum lycopersicum var. cerasiforme) (Group=CER) 5개의 계통으로 구성되어있다. 124 개체에서의 다형성 조사 결과와 동일하게 각 계통의 교배조합 내에서 다형성을 나타내는 마커의 개수를 상자의 진하기와 크기로 표시하였다.
도 5는 본 발명의 주요 육종자원 교배 조합 내 MABC용 SNP 후보를 이용하여 다형성 (polymorphism) 검증을 수행한 결과를 나타낸다.
도 6은 Luna probe를 이용하여 SNP 검증을 수행한 결과를 나타낸다. 분석과정의 정확도를 평가하기 위하여 MABC용 SNP 후보 선발과 별도로 토마토 유전체 내 18개의 Luna probe용 SNP를 선발하여 검증하였으며, 도 6은 전체 18개의 마커 중 2개 마커에 대한 결과를 나타낸다.
본 발명의 목적을 달성하기 위하여, 본 발명은 SNP(single nucleotide polymorphism) 위치 염기를 포함하는 서열번호 1 내지 143의 염기서열로 이루어진 폴리뉴클레오티드로부터 선택되는 하나 이상의 폴리뉴클레오티드 또는 이의 상보적 폴리뉴클레오티드를 포함하는, 마커이용여교잡(MAB, marker assisted backcross) 육종을 위한 토마토의 유전적 배경 또는 품종 진단용 SNP 조성물을 제공한다.
상기 서열번호 1 내지 143은 상기 각각의 SNP 부위의 염기서열을 포함하는 폴리뉴클레오티드이다. 상기 서열번호 1 내지 143은 다형성 부위를 포함하는 다형성 서열이다. 다형성 서열(polymorphic sequence)이란 폴리뉴클레오티드 서열 중에 SNP를 나타내는 다형성 부위(polymorphic site)를 포함하는 서열을 말한다. 상기 폴리뉴클레오티드 서열은 DNA 또는 RNA가 될 수 있다.
본 발명의 일 구현 예에 따른 SNP 조성물에 있어서, 상기 SNP 위치 염기는 서열번호 1 내지 143의 염기서열로 이루어진 폴리뉴클레오티드에 있어서, 각각의 염기서열 중 서열번호의 151번째 염기에 위치하며, 이러한 다형성 염기 정보는 표 8의 SNP 염기서열 정보에 []로 표시하였다.
본 발명에 따른 마커이용여교잡 육종을 위한 토마토의 유전적 배경 또는 품종 진단용 SNP를 구성하는 각 단일 SNP의 폴리뉴클레오티드는 바람직하게는 10개 이상의 연속 염기이며, 더욱 바람직하게는 15개 이상의 연속 염기이며, 더더욱 바람직하게는 20개 이상의 연속 염기이며, 가장 바람직하게는 서열번호 1 내지 143의 각 서열이다.
본 발명은 또한, 상기 SNP의 폴리뉴클레오티드, 이에 의해 코딩되는 폴리펩티드 또는 이의 cDNA를 포함하는 마커이용여교잡 육종을 위한 토마토의 유전적 배경 또는 품종 진단용 마이크로어레이를 제공한다. 바람직하게는, 상기 폴리뉴클레오티드는 아미노-실란, 폴리-L-라이신 또는 알데히드의 활성기가 코팅된 기판에 고정될 수 있으나, 이에 제한되지는 않는다. 바람직하게는, 상기 기판은 실리콘 웨이퍼, 유리, 석영, 금속 또는 플라스틱일 수 있으나, 이에 제한되지는 않는다. 상기 폴리뉴클레오티드를 기판에 고정화시키는 방법으로는 피에조일렉트릭(piezoelectric) 방식을 이용한 마이크로피펫팅(micropipetting) 법, 핀(pin) 형태의 스폿터(spotter)를 이용한 방법 등을 사용할 수 있다.
본 발명에 따른 마이크로어레이는 본 발명에 따른 폴리뉴클레오티드 또는 그의 상보적 폴리뉴클레오티드, 그에 의해 코딩되는 폴리펩티드 또는 그의 cDNA를 이용하여 본 분야의 당업자에게 알려져 있는 통상적인 방법에 의해 제조될 수 있다.
또한, 본 발명은 상기 마이크로어레이를 포함하는 마커이용여교잡 육종을 위한 토마토의 유전적 배경 또는 품종 진단용 키트를 제공한다.
본 발명에 따른 키트는 본 발명의 마이크로어레이 이외에 피검체로부터 해당 SNP를 포함하는 DNA를 분리 및 증폭하는데 사용되는 프라이머 세트를 추가로 포함할 수 있으나, 이에 제한되지는 않는다.
또한, 본 발명은
토마토 시료에서 게놈 DNA를 분리하는 단계; 및
본 발명의 서열번호 1 내지 143의 염기서열로 이루어진 폴리뉴클레오티드의 각 SNP(single nucleotide polymorphism) 위치 염기인 다형성 부위의 유전자형을 결정하는 단계를 포함하는 마커이용여교잡 육종을 위한 토마토의 유전적 배경 또는 품종 진단 방법을 제공한다.
본 발명의 방법에 있어서, 토마토 시료로부터 게놈 DNA를 분리하는 방법은 당업계에 알려진 통상적인 방법을 통하여 이루어질 수 있다. 예를 들면, 조직 또는 세포로부터 DNA를 직접적으로 정제하거나 PCR과 같은 증폭 방법을 사용하여 특정한 영역을 특이적으로 증폭하고 이를 분리함으로써 이루어질 수 있다. 본 발명에 있어서, DNA란 DNA 뿐만 아니라 mRNA로부터 합성되는 cDNA도 포함한다. 피검체로부터 핵산을 얻는 단계는 예를 들면, PCR 증폭법, 리가제 연쇄 반응(LCR)(Wu 및 Wallace, Genomics 4, 560(1989), Landegren 등, Science 241, 1077(1988)), 전사증폭(transcription amplification) (Kwoh 등, Proc. Natl. Acad. Sci. USA 86, 1173(1989)), 자가유지 서열복제 (Guatelli 등, Proc. Natl. Acad. Sci. USA 87, 1874(1990)) 및 핵산에 근거한 서열 증폭 (NASBA)이 사용될 수 있으나, 이에 제한되지는 않는다.
분리된 DNA의 염기서열의 결정은 당업계에 알려진 다양한 방법에 의하여 이루어질 수 있다. 예를 들면, 디데옥시법에 의한 직접적인 핵산의 뉴클레오티드 서열의 결정을 통하여 이루어지거나, SNP 부위의 서열을 포함하는 프로브 또는 그에 상보적인 프로브를 상기 DNA와 혼성화시키고 그로부터 얻어지는 혼성화 정도를 측정함으로써 다형성 부위의 뉴클레오티드 서열을 결정하는 방법 등이 이용될 수 있으나, 이에 제한되지는 않는다. 상기 혼성화의 정도는 예를 들면, 검출가능한 표지를 표적 DNA에 표지하여, 혼성화된 표적 DNA 만을 특이적으로 검출함으로써 이루어질 수 있으며, 그외 전기적 신호 검출방법 등이 사용될 수 있으나, 이에 제한되지는 않는다. 본 발명에 따른 마커이용여교잡 육종을 위한 토마토의 유전적 배경 또는 품종 진단 방법은 바람직하게는, 상기 토마토 시료로부터 분리한 핵산 시료를 본 발명에 따른 SNP를 포함하는 폴리뉴클레오티드 또는 이의 상보적 폴리뉴클레오티드, 또는 이와 혼성화하는 폴리뉴클레오티드와 혼성화시킨 후 혼성화 결과를 검출하는 단계를 포함할 수 있다.
이하, 본 발명을 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.
재료 및 방법
1. 토마토 유전체 정보의 확보 및 특징 분석
현재 재배되고 있는 토마토의 품종은 솔라눔 리코퍼시쿰(Solanum lycopersicum)에 속해 있으며, 야생종 토마토에서의 병저항성과 같은 유용한 형질을 도입하기 위하여 유전자 및 염색체의 일부를 도입하여 육성되고 있다. 토마토 유전체 시퀀싱 데이터는 다양한 국가의 연구진에 의하여 생산되어 NCBI의 SRA(Sequence Read Archive) 데이터베이스에 약 800여 개의 SRA 정보가 집적되어 있으며 본 연구를 위해 수집한 염기서열 길이(bp)는 약 7.8Tb에 해당한다.
NCBI-SRA에 등록된 토마토 유전체(tomato genome) 데이터
토마토 SRA 데이터의 학명 SRA 데이터 Total bases (bp)
Solanum lycopersicum 557 5,367,216,027,209
Solanum lycopersicum var. cerasiforme 133 818,234,753,829
Solanum pimpinellifolium 65 470,510,067,600
Solanum habrochaites 17 324,511,688,762
Solanum pennellii 14 137,734,433,883
Solanum arcanum 12 165,974,167,372
Solanum huaylasense 3 101,084,736,600
Solanum cheesmaniae 2 68,609,466,800
Solanum chilense 2 64,731,490,900
Solanum chmielewskii 2 67,986,640,700
Solanum neorickii 2 70,608,085,300
Solanum peruvianum 2 69,515,482,100
Solanum corneliomuelleri 1 32,520,818,500
Solanum galapagense 1 30,910,824,200
총 813 7,790,148,683,755
2. 토마토 집단을 대변하는 계통의 선정
발표된 논문(Tao Lin et al. 2014 Nature Genetics 46, 1220-1226)을 참고하여 연구 및 분석에 사용 가능한 형태의 염기서열 데이터로 토마토 234개 계통의 염기서열 데이터를 선정하였다. 분석에 사용된 토마토 SRA 데이터의 정보는 아래 표 2와 같다. 계통 선발은 수집된 토마토 SRA 계통 중 대과종 완숙용 또는 가공용 재배종으로 많이 이용되고 있는 솔라눔 리코퍼시쿰을 우선으로 하였고, 방울토마토 재배종인 솔라눔 리코퍼시쿰 변종 세라시포르메(Solanum lycopersicum var. cerasiforme)와 방울토마토 육종과정에서 활용도가 높은 솔라눔 핌피넬리폴리움(Solanum pimpinellifolium)을 기본으로 하여 일부 야생종 토마토 계통을 포함하였다.
분석에 사용된 토마토 SRA 정보
토마토 SRA 데이터의 학명 분석에 사용된 SRA 개수
Solanum lycopersicum 126
Solanum lycopersicum var. cerasiforme 68
Solanum pimpinellifolium 33
Solanum habrochaites 1
Solanum cheesmaniae 2
Solanum chilense 1
Solanum neorickii 1
Solanum peruvianum 2
총 234
3. 토마토 표준 유전체 염기서열 데이터의 수집
토마토 표준유전체(reference genome)은 SGN (ftp://ftp. solgenomics .net/)에서 수집한 솔라눔 리코퍼시쿰(Solanum lycopersicum) Heinz 1706 (ITAG 버전 2.4)을 사용하였다. 표준 유전체는 12개 염색체 (chromosome)과 12개 염색체 중 하나의 염색체로 자리 잡지 못한 스캐폴드 (scaffold) 염기서열들을 묶은 1개의 기타 그룹으로 구성된 13개의 염색체로 구성되어 있으며, 서열의 총 길이는 781,666,411 bp에 해당한다.
분석에 사용된 토마토 표준유전체 정보(reference genome: ITAG 버전 2.4)
No. of chromosome Total Length (bp) N50 length (bp) Avg. length (bp)
13 781,666,411 64,840,714 60,128,185
4. 토마토 MABC 용 SNP 후보 선발
공개 데이터베이스(NCBI SRA)에서 수집한 토마토 234 계통의 유전체 염기서열 데이터를 이용하여 유전자 하플로타입(haplotype) 분석을 통한 tag-SNP 선발을 통해 27,680좌의 Tag-SNP를 선발하였다. 27,680좌의 Tag-SNP 중 토마토 MABC 용 SNP 후보를 선발하였다. Tag-SNP의 염색체 분포를 확인한 이후 염색체 영역별로 마커를 선발하였다. 염색체의 길이를 고려하여 5Mb에서 10Mb 간격으로 영역을 나누었으며 해당 영역에서 PIC value가 높은 SNP를 선발하였다. 한 염색체 당 최소 10개의 SNP를 선발하였으며 143개의 후보 마커를 선발하였다.
4.1. 234 계통 중 주요 육종자원의 교배 조합 작성
234개의 토마토 계통의 경우 재배종으로 이용되고 있는 솔라눔 리코퍼시쿰(Solanum lycopersicum) 이외에도 유전적 배경이 다양한 야생종들이 포함되어 있기 때문에 선발된 MABC(Marker-assisted Backcrossing)용 마커가 실제 토마토 육종 과정에서 활용 가능한지 확인하기 위하여 전체 234개의 계통 중 주요 육종자원으로 이용되는 토마토의 계통을 선발하였다. 1차로 선발된 234개의 토마토 SRA 계통 중 대과종 완숙용과 가공용 재배종으로 많이 이용되고 있는 솔라눔 리코퍼시쿰(Solanum lycopersicum)을 우선으로 하여 방울(체리) 토마토로 알려져 있는 솔라눔 리코퍼시쿰 변종 세라시포르메(Solanum lycopersicum var. cerasiforme)와 육종과정에서 활용도가 높은 솔라눔 핌피넬리폴리움(Solanum pimpinellifolium)에 해당하는 계통을 위주로 하여 교배 조합을 작성하였다.
샘플 124개 선정( 참고문헌: doi :10.1038/ng.3117 )
NCBI SRA로부터 얻은 샘플 234개 샘플 중에서 확보한 유전체 데이터의 양과 퀄리티가 충분하고 주로 육종의 소재로 이용되는 솔라눔 리코퍼시쿰(Solanum lycopersicum) 및 리코퍼시쿰 변종 세라시포르메(Solanum lycopersicum var. cerasiforme) 토마토 품종을 일차적으로 178개 계통을 선발하였으며, 선발된 178개 샘플에서 토마토 대과종과 소과종으로 구분되는 group=BIG lines 94 샘플과 group=CER lines 68 샘플 총 162개 샘플을 선발하였고, 최종적으로 총 162개 샘플 중에서 각 그룹 내 사용 용도에 따른 다양한 카테고리에 해당하는 개체들 중 랜덤(random)하게 124개 샘플을 최종 선발하여 MABC 마커 선발에 이용하였다.
토마토 사용 용도에 따른 카테고리 분류
No. Categories
1 cultivar
2 Cocktail tomato
3 Vintage cultivar
4 Latin American cultivar; Wild species
5 Latin American cultivar
6 landrace
7 Vintage Processing
8 Vintage Fresh Market
9 Inbreed line
10 Modern Fresh Market
11 Processing tomato
12 Fresh Market
13 Processing
14 Landrace/Latin American cultivar
15 Vintage Fresh Market,Monogenic
16 Landrace/Latin American cultivar
17 Cocktail/Processing tomato
최종적으로 선발된 124개 샘플의 정보는 아래 표 5에 나타내었다. 각 샘플의 SRA 정보와 품종 정보를 나타내었으며 Group은 BIG과 CER 두 가지로 분류하였다. 카테고리는 위의 표와 같이 사용 용도에 따라 17가지로 분류하였으며 육종을 통하여 개량된 품종의 경우 품종명을 표기하였다. 124개 샘플은 group=CER lines 39 샘플과 group=BIG lines 85 샘플로 구성되어 있다(도 1).
MABC 마커 선발에 이용된 124개 샘플 정보
No SRA Variety Group Categories Name(cultivar)
1 SRR1572327 S. lycopersicum var cerasiforme CER cultivar cerise rose
2 SRR1572335 S. lycopersicum var cerasiforme CER Cocktail tomato
3 SRR1572339 S. lycopersicum var cerasiforme CER Cocktail tomato
4 SRR1572343 S. lycopersicum var cerasiforme CER cultivar Ohmiya SunCerise
5 SRR1572344 S. lycopersicum var cerasiforme CER Vintage cultivar Principe Borghese
6 SRR1572345 S. lycopersicum var cerasiforme CER cultivar Poire jaune
7 SRR1572346 S. lycopersicum var cerasiforme CER cultivar
8 SRR1572348 S. lycopersicum var cerasiforme CER LatinAmericancultivar;Wild species Trujillo
9 SRR1572362 S. lycopersicum var cerasiforme CER cultivar
10 SRR1572364 S. lycopersicum var cerasiforme CER Cocktail tomato
11 SRR1572365 S. lycopersicum var cerasiforme CER cultivar N1565
12 SRR1572372 S. lycopersicum var cerasiforme CER LatinAmericancultivar;Wild species Moyobamba
13 SRR1572373 S. lycopersicum var cerasiforme CER cultivar Cuba Plum
14 SRR1572375 S. lycopersicum var cerasiforme CER cultivar Malintka 101
15 SRR1572377 S. lycopersicum var cerasiforme CER LatinAmericancultivar Villa Hermosa
16 SRR1572380 S. lycopersicum var cerasiforme CER LatinAmericancultivar Veracruz
17 SRR1572383 S. lycopersicum var cerasiforme CER cultivar Monplaisir
18 SRR1572384 S. lycopersicum var cerasiforme CER cultivar Nagcarlang
19 SRR1572385 S. lycopersicum var cerasiforme CER LatinAmericancultivar
20 SRR1572386 S. lycopersicum var cerasiforme CER cultivar 8 bis
21 SRR1572390 S. lycopersicum var cerasiforme CER cultivar Phyra
22 SRR1572391 S. lycopersicum var cerasiforme CER landrace Mobalcon
23 SRR1572396 S. lycopersicum var cerasiforme CER cultivar Atom
24 SRR1572400 S. lycopersicum var cerasiforme CER cultivar L 285
25 SRR1572401 S. lycopersicum var cerasiforme CER LatinAmericancultivar
26 SRR1572406 S. lycopersicum var cerasiforme CER cultivar N 795 Pescio
27 SRR1572409 S. lycopersicum var cerasiforme CER cocktail tomato
28 SRR1572413 S. lycopersicum var cerasiforme CER cultivar Minibel
29 SRR1572417 S. lycopersicum var cerasiforme CER landrace Linosa
30 SRR1572424 S. lycopersicum var cerasiforme CER landrace Cerazjnho
31 SRR1572425 S. lycopersicum var cerasiforme CER landrace Allungato piccolo
32 SRR1572426 S. lycopersicum var cerasiforme CER Cocktail tomato
33 SRR1572427 S. lycopersicum var cerasiforme CER LatinAmericancultivar
34 SRR1572430 S. lycopersicum var cerasiforme CER cultivar N 933
35 SRR1572442 S. lycopersicum var cerasiforme CER Latin American cultivar Tarapoto
36 SRR1572446 S. lycopersicum var cerasiforme CER cultivar
37 SRR1572449 S. lycopersicum var cerasiforme CER cultivar BL 587-S
38 SRR1572450 S. lycopersicum var cerasiforme CER cultivar BL 587
39 SRR1572451 S. lycopersicum var cerasiforme CER cultivar BL 359
40 SRR1572452 S. lycopersicum BIG Vintage Processing VF-36
41 SRR1572453 S. lycopersicum BIG Vintage Fresh Market Moneymaker
42 SRR1572455 S. lycopersicum BIG Inbreed line Hawaii 7998
43 SRR1572456 S. lycopersicum BIG Vintage Fresh Market Edkawi
44 SRR1572457 S. lycopersicum BIG Vintage Processing San Marzano
45 SRR1572458 S. lycopersicum BIG Modern Fresh Market Micro-Tom
46 SRR1572460 S. lycopersicum BIG Vintage Fresh Market AilsaCraig
47 SRR1572461 S. lycopersicum BIG Vintage Fresh Market Marglobe
48 SRR1572462 S. lycopersicum BIG Modern Fresh Market KR2
49 SRR1572463 S. lycopersicum BIG Modern Fresh Market yoku improvement
50 SRR1572464 S. lycopersicum BIG Cocktail tomato
51 SRR1572465 S. lycopersicum BIG Processing tomato
52 SRR1572466 S. lycopersicum BIG Fresh Market Moneymaker
53 SRR1572467 S. lycopersicum BIG Landrace A pera abruzzese
54 SRR1572479 S. lycopersicum BIG Vintage Fresh Market New Yorker
55 SRR1572482 S. lycopersicum BIG LatinAmericancultivar Huachinango
56 SRR1572484 S. lycopersicum BIG Fresh Market N739
57 SRR1572485 S. lycopersicum BIG Processing tomato
58 SRR1572487 S. lycopersicum BIG Processing tomato
59 SRR1572488 S. lycopersicum BIG Processing tomato
60 SRR1572489 S. lycopersicum BIG Processing tomato
61 SRR1572495 S. lycopersicum BIG Processing tomato
62 SRR1572496 S. lycopersicum BIG Cocktail tomato
63 SRR1572497 S. lycopersicum BIG Processing tomato
64 SRR1572498 S. lycopersicum BIG Fresh Market Moneymaker
65 SRR1572499 S. lycopersicum BIG Processing
66 SRR1572514 S. lycopersicum BIG Landrace Scatolone di bolsena
67 SRR1572515 S. lycopersicum BIG Landrace/LatinAmericancultivar Hacienda Calera
68 SRR1572516 S. lycopersicum BIG Vintage Processing Pearson
69 SRR1572517 S. lycopersicum BIG Modern Fresh Market Severianin
70 SRR1572518 S. lycopersicum BIG Vintage Fresh Market Primabel
71 SRR1572530 S. lycopersicum BIG Landrace/LatinAmericancultivar Hacienda Rosario
72 SRR1572532 S. lycopersicum BIG Landrace Spagnoletta
73 SRR1572533 S. lycopersicum BIG Vintage cultivar Red Setter
74 SRR1572534 S. lycopersicum BIG cultivar 149-77
75 SRR1572535 S. lycopersicum BIG cultivar Saladette
76 SRR1572536 S. lycopersicum BIG Vintage cultivar Roma
77 SRR1572537 S. lycopersicum BIG Modern Fresh Market Florida7547
78 SRR1572545 S. lycopersicum BIG Landrace/LatinAmericancultivar Santa Cruz B
79 SRR1572548 S. lycopersicum BIG Vintage Fresh Market,Monogenic Condine Red
80 SRR1572549 S. lycopersicum BIG Processing tomato
81 SRR1572550 S. lycopersicum BIG Processing tomato
82 SRR1572551 S. lycopersicum BIG Processing tomato
83 SRR1572552 S. lycopersicum BIG Processing tomato
84 SRR1572558 S. lycopersicum BIG Landrace/LatinAmericancultivar Tegucigalpa
85 SRR1572561 S. lycopersicum BIG Landrace Cuor di bue
86 SRR1572564 S. lycopersicum BIG Processing tomato
87 SRR1572566 S. lycopersicum BIG Processing tomato
88 SRR1572567 S. lycopersicum BIG Processing tomato
89 SRR1572568 S. lycopersicum BIG Processing tomato
90 SRR1572569 S. lycopersicum BIG Processing tomato
91 SRR1572570 S. lycopersicum BIG Processing tomato
92 SRR1572573 S. lycopersicum BIG Processing tomato
93 SRR1572574 S. lycopersicum BIG Processing tomato
94 SRR1572583 S. lycopersicum BIG Landrace Francescano
95 SRR1572584 S. lycopersicum BIG Vintage cultivar Severianin
96 SRR1572585 S. lycopersicum BIG Landrace Pantano dArdea
97 SRR1572587 S. lycopersicum BIG Vintage Fresh Market Ace
98 SRR1572588 S. lycopersicum BIG Processing tomato N020212
99 SRR1572589 S. lycopersicum BIG Landrace Libanese
100 SRR1572593 S. lycopersicum BIG Landrace/Latin American cultivar San Salvador
101 SRR1572594 S. lycopersicum BIG Landrace Bell pepper-like
102 SRR1572595 S. lycopersicum BIG Modern Fresh Market Florida7060
103 SRR1572596 S. lycopersicum BIG Vintage Fresh Market PrinceBorghese
104 SRR1572597 S. lycopersicum BIG Modern Fresh Market NC265-1(93)-3-3
105 SRR1572607 S. lycopersicum BIG cultivar Barnaulski Konservnyi
106 SRR1572610 S. lycopersicum BIG Processing tomato
107 SRR1572611 S. lycopersicum BIG cultivar Microtom
108 SRR1572615 S. lycopersicum BIG Processing
109 SRR1572616 S. lycopersicum BIG Processing tomato
110 SRR1572617 S. lycopersicum BIG Processing tomato
111 SRR1572618 S. lycopersicum BIG Processing tomato
112 SRR1572619 S. lycopersicum BIG Vintage Fresh Market Platense
113 SRR1572620 S. lycopersicum BIG Modern Fresh Market NC EBR-5
114 SRR1572623 S. lycopersicum BIG LatinAmericancultivar Ayacucho
115 SRR1572625 S. lycopersicum BIG Processing tomato
116 SRR1572626 S. lycopersicum BIG Landrace/LatinAmericancultivar Merida
117 SRR1572629 S. lycopersicum BIG Processing tomato
118 SRR1572630 S. lycopersicum BIG LatinAmericancultivar
119 SRR1572631 S. lycopersicum BIG Processing tomato
120 SRR1572654 S. lycopersicum BIG Landrace Ucra2
121 SRR1572655 S. lycopersicum BIG Processing tomato
122 SRR1572656 S. lycopersicum BIG Processing tomato
123 SRR1572658 S. lycopersicum BIG Cocktail/Processing tomato
124 SRR1572666 S. lycopersicum BIG Landrace
MABC 143개 선발
선발과정 선발된 샘플 개수 마커 선발 개수
1 Tag SNP 27,680
2 Group=BIG+CER:missing data < 30%
Group=BIG:PIC > 0.2
Group=CER:PIC > 0.2		 4,274
3 염색체 일정 크기단위로 마커 10개씩 선발 (chr. 12 x 10 = 120)
(5~10mb 단위)		 143
4 Luna probe 검증 18
전 연구에서 공개 데이터베이스(NCBI SRA)에서 수집한 토마토 234 계통의 유전체 염기서열 데이터를 이용하여 유전자 하플로타입 분석을 통한 tag-SNP 선발을 통해 27,680좌의 Tag-SNP를 선발하였다. 27,680좌의 Tag-SNP 후보 마커들 중에서 다양한 토마토 집단들의 교배조합을 이용한 여교배선발(MABC)용 SNP 마커 후보를 최종 선발하였다. 후보 SNP 마커인 27,680개의 Tag-SNP를 이용하여 Group 전체의 missing data가 30% 이내이고, 각 그룹 내에서 PIC(polymorphism information content) 값이 0.2보다 큰 4,274개의 SNP 마커를 일차적으로 선발하였다.
PIC value는 다음의 식으로 계산하였다.
Figure 112017048004187-pat00001
일차적으로 선발된 4,274개의 SNP 마커를 후보로 하여 염색체 5Mb 크기 단위로 총 143개의 MABC 마커를 선발하였다. 5Mb 내에서 PIC 값이 0.2 이상인 SNP를 후보로 하였으며 124개의 교배조합 내에서 다형성을 가장 많이 나타내는 SNP를 선발하였다. 두 번째 영역부터는 앞서 선발된 SNP 마커가 구분하지 못하는 교배조합을 구분할 수 있는 수가 많은 SNP 마커를 선발하였다.
실시예 1. 본 발명의 통해 선발된 143개 MABC 마커
선발된 143개 마커에 대한 정보는 아래 표 7에 나타내었다. 'No. of candidate primer'에서 MABC 마커 선발에 후보로 이용된 27,680개의 Tag-SNP의 염색체별 개수를 나타내었으며 'No. of selected SNP marker'에서 최종적으로 선발된 마커의 개수를 표기하였다. 평균적으로 10~11개의 마커 선발을 목표로 하였다. 선발된 마커들의 위치에 따라 Intergenic과 Genic(Promoter+Intron+CDS) 영역으로 분류하였다. 염색체 5번, 10번, 12번의 마커를 제외하고 선발된 마커들은 유전자 영역 내에 위치함을 확인할 수 있다. 'Average PIC value'에서는 한 염색체 내 MABC 마커의 평균 PIC value를 나타내었다.
MABC 요약
Chr.# No. of candidate primer No. of selected SNP marker Intergenic Genic (Promoter+Intron+CDS) Average PIC value
1 3488 19 - 19 0.374
2 2780 10 - 10 0.372
3 2612 13 - 13 0.365
4 2394 13 - 13 0.370
5 2066 12 - 12 0.370
6 2214 10 - 10 0.371
7 1963 11 - 11 0.355
8 2027 8 - 8 0.368
9 1911 11 - 11 0.373
10 1963 11 - 11 0.370
11 1944 11 - 11 0.365
12 1948 14 - 14 0.373
Total 27310 143 - 143 0.369
선발된 MABC 마커 정보
서열번호 SNP ID Chr Position Gene Allele PIC value Flanking sequence(301bp)
1 1-1 SL2.40ch01 818648 Solyc01g006180.2 T/C 0.38 TAGTAGTGCCCCAAAACAAATAAGAGAGACGAGTAGAATACAACTATCTAATCAACATCACATTAGGAGAGACGAGAAGAATGCACCTATCTAATCAACATCACAATAAGAGATGAGACGAATGCAATTATCTAATCAATATCATAATTC[T/C]GACCTTTATTGGTCGGTATGCCATAATAGCCCCCATGTGCCCCAAGTCAAATGAGAGTAATGAGAAAAATGCAACTATCTAATCAGGATCACAGTTTCGACCTTTGTTCGTTAATTAACTCTATTAGTCTTCCAAGCCAAATATAAGAGG
2 1-2 SL2.40ch01 7603608 Solyc01g011090.2 A/G 0.38 AAATTTGATGTCTCATTGAATCAAGATCAGATGGAAGTCCAGCAAGTGTAACAACTAGAAACGTCTTCTGACACTGCTCTAATTGCTTTTCAACATTTGGAGTAGGGGGCATTAACTCATTAAGTTCCAGCTTTTTAAGATTAGTCAACC[A/G]AGAAATCACATTATAAAAGCAGGATATATCATTTGTGTACAGCCCACGAACCTTTTCCCAAACTAAAAAGTAAGTCTGGAATGGTCGAAACAATGGCATCAACTTTGATCAATAGATTTCCATAACAGACTACACAATTGAGCATCAATC
3 1-3 SL2.40ch01 10369682 Solyc01g013910.1 A/C 0.37 CCAAAAATAAGTAATAAGTCTTGTAACCAGCCTCGAATATTTGAAATAGAGATATGTTGTAACAACATAATCTAGCATTACTTCTCCAAGAAATTTCAAGTTCTAATAGAATCGTGGAATCTCATGTAGCACGTAAGATCTGTGAGCTAG[A/C]GTTTTAACAAGCAGCGAAGGGTCGTGAAACTTTTAGTGTAGCAGTGATTTCTATGTACCGTACATTGACTAGCTTCTTAGCATTCACGATGAAGTGTATTAAGATAGGTGCATCAATAAAATCAATATCCATCTAGATCCATTTCATAAG
4 1-4 SL2.40ch01 15820305 Solyc01g014910.1 A/G 0.37 CCTTTATTGTATTTTTTTTATTAAATAAATTTTTTTCAAACAATAATAAATGACTTGAAAGTAATAAATTTGTGTGTATATATATAGTAGATATGGTATTCCTTTGACTTCTTCGTATGTATTTACTTTCCGTTAATCAGTGAAAATCTC[A/G]ACTTTCCCTCTGACATAGAAATTAAATTAATTTCATATTTTTGTAAATGCAGGCTGATGAGTGCACCATTATTGATGAAGCAGTGAACCACATCCTAAAGCTGAAGAATACTTTTGAGAAGCTTAAACAGAAAAAGCTAGATGATCTTCA
5 1-5 SL2.40ch01 23787004 Solyc01g017040.1 T/C 0.38 TCAATTCATGTGAAAAGGACCCAACTATGAACAAGAAGAGGAGAGAGCAGATGGAGACTGTGGATAACAATTTATGAAGAAAAAGGATTGACAGAAATAGCCCTTGAAATTCAATGTTTTTACAGGGAAGCACACGCGTTGACGGAGACA[T/C]GTGTTCTATTCCCTCTTCTATATCGTAATATGATATTTATCATCGTGGTCGATTCTATAAATTTAAAAATAAAGGGATAATGCATAAGTACCCCCTAAACCTATGACTGAAATCTCAGAGACACACTTATACTATACTAAGGTCCTATTA
6 1-6 SL2.40ch01 25772669 Solyc01g017850.1 T/C 0.38 AGCAAAGATTCCAGATAAATATGAGTATCCTATATTGCATAAATTAGTGCTCAAACATATGATGCATGGTCCATGTGGAAACAAACGTCCAACAAAAAAATGCATGCAAAATGGACAGTGTAAATATCATTATCCAAGGCCATACAACAA[T/C]AAATCAATCCAAGCAAAGGATGGATATCCAATTTATAAAAGAAGAATGGATGGAAGAATAGAGATTGTTCGTGGAATGCATATGACAAATAGTAACTTACAACCCTTCTCTGCTTACAAGATACAATTGTCACATATGTTGAGGTTTGCT
7 1-7 SL2.40ch01 30941371 Solyc01g020460.1 T/C 0.37 TGTTTTGGTGGCAGGTACTAGTACATCGAGGCCTTAGAGAGCCGCTGGACTCCAGACAAGCCGTTACGAAGCGCCATCATCCCAGGTCAGGCGACCCGAAAGGACCAGACATAAACCACCGCTAAGAAAAGGGTAAATATGACAAGCAAT[T/C]AAGCTGGCAAGTCAGCGATACAGGATTGATTTTTCCAGGGTGTGTGAACTTCCTTATATATTCAGCCACATGTGTGGGTGTGAAAGGATTTTATGGTCTCGGTCATTCTTCTATGAAATATCGATTCTATCAATCATTCCATTTCAGAAT
8 1-8 SL2.40ch01 38118538 Solyc01g044270.2 T/C 0.38 CAGGAAGAAACAGTTCAAGCGCTCCAAATTTTGTGTCGTCTGTGGTCAATAACACTACGTCACCTGATCACAATAACTTCCAGTGGAGCCCATTTTCCCAAACCGCTGGGCATGCATCTGCTGCACAAGCAGCTAATATGGTACAGCAAG[T/C]GTATCAGAAAGTAAAACGAGAGCGTCAAGAAGCCCAGACAGCAACGAAAAGAGAGGAAGCATTGAAAAGGAAAAATCATTCGTCCAAACGACCAAGTGCAGCTTTATCAGCTGGACAGTTTAATGCTTTCAAGAGGAAAAAAGGCATAAA
9 1-9 SL2.40ch01 40895601 Solyc01g049680.2 A/G 0.37 TAGCATTTTAAAAGAATTTGTAATCGTAGGTATCTCAGTAGCTTTTAAGAAACCATACAATACTCCTAAGTGGGTTTGGTTTCAAGGTGGGTCTTAAAGATGTACCAGAAATTGGAGTTAGTTCCAGAAAGGTTTCACCATTTTCATTTA[A/G]AGAAAGTTAACTGGTATTATAAACAGAAAGAAATACACCAATAGTGTACTTCTGTAGTAATTACATAAAAAAGTGAAGAGAGAGCCCATTTGGAAAAAAAAAAGAGATCCATCACCTTTCTGCCTCAGAAGCTATCAAAAATTAGGGTAA
10 1-10 SL2.40ch01 46781031 Solyc01g056370.2 A/C 0.37 TTTTGGCACCTCAATCCTCACGGTAATAATTATGCCAATTCTACCTAAAAATCTCTTTAGGTGATCAAGGGGGCTCCCTTTTTCAGTAGATGAGATTAGGATGAAAGGTATTATCTCAACGCCTCTTTTCTCCGGCTTGTTCTTTCAAAT[A/C]CAATTTTGGCCTACCCAAACTTTTCTGGTTAACCCCAACATTCCCCACTTGTCCAAGACTCTTCTTCAGACTTAGATTCTCTAGCTTCTATTCTCGGGGTACGTAGATCACTAGGTAGCCTAGCTCGACCCAAGCAATGCCCAAAAGTCC
11 1-11 SL2.40ch01 54848293 Solyc01g057580.1 G/C 0.38 ATAGCTGATGCAAGTTGTTTTTCTTGTAGTGTTGTTTGTACTACTACTTTTTGATCTAGCTGTTAGTTTGTTCAATTTCTTCAGATCCGAATAGTGCTTGTTTTCATTGGCGTCAAGTGATGTTGGACTTGTCAAAGCTACTGTTCTTCC[G/C]GTGTTGTGCAAGGTTATCAAAGCGGGGCCAGTCTTTGAAGCTGAAGAATTTAACCATTTTATTTTTTTTCTTGTGCATCACTAATGCTGAGTTGCCTGATGAGCCATTTGAGTAACAACCTGTTACTCCAGTCAATAAACATGTTGAAGC
12 1-12 SL2.40ch01 59236771 Solyc01g058360.1 T/C 0.38 AATGAATCAGCCCCAAAAGAAAATCTTTTTAAGAACTGGCCGTTGATGTCATCTATCATATCATACTGCATTTTCGCTCTTCATGATATGGCGTACTCAGAGGTATACCTATATATATCACTAATAAGTCTCATTCTAAGAGTAGCTGAT[T/C]AGACTCAGGGACGTTCCTTTGAGGAAACACACAAACGTTCTTTCATTTCTCACATTCATTCGAATCCAATGTGTCGTCAAACCTTCTTTTAATTTCTGGAGTGACTTGAATAGGGCATCTTGATTCTTTTGCAGGAAGAACTGTAACACA
13 1-13 SL2.40ch01 64181765 Solyc01g065520.1 C/T 0.38 GCTTGATATAGAGTGCTGGAAGTTTTATAAGGACCAGTTGTAGGGATGGAAGGAAAAGTAGAAGTAGGAGGCTGATTATTAGGGTTAGAAGTCTCATTAAAAGATTCCTCACCTACCCCACTTTCAGTCACGTGAGGGGAAAACTCCAAC[C/T]CAAGTGGGGCGTGATTAGCTTCACTAACCCCATGAAAAATATACACATACATTGTACCCCCCATAATTTATCATATTGAAAAGGTCAAAATAACCTTATCTTTATCAACAAGGACCAATAACCCATCCCACTTGATGAAAAAATCACAGT
14 1-14 SL2.40ch01 65623707 Solyc01g066170.1 A/C 0.37 CATAATTATATTTCATTTTTTATATTGTTTAGATTTATATCATTTCTTACTATGTTTTCATTTTGTTTATAGCACAGCGATGTGATTTTTTACTACCTTTGTAAAAAATCAAAACTTTGCAGCATGGATCAATACAGATACACAACAACC[A/C]ACTGTTTGTTCATGTCACATGTAAAAAACTGTTATGATAGATATTACATGGACGATGATGATAGTCTTACTACACAAGAACATATTGATCGCGCTTCAATTGTATCTGTACATGAGAGGTCAATTATCAACATCATCAAAGGGTTTGGAA
15 1-15 SL2.40ch01 73007858 Solyc01g081210.2 A/G 0.38 AGCAATAAGAATCATTCGTTCTGCAACAGTTAACGATTGAATCGAAAGACTCCTTGATATAAAAGACGGATGATGGCGTAGTATATGAATTGGTGGATCTGACGTAGGCGGATTTGGTAACTGAGTCCACTTTGATGAAATTGGATCTAA[A/G]CAGAAAAACTCCAAATTACTAGTAGTGATAGTGCTATTAGTACTAGTACTGTTGTTTTTGTTGTATGCTGTTGAAGTAGCAGTACTGTTACTACTTCGAGAGCATACAGCGTAAAGACAGTAAAACAGAGGAAAAGATGAGGTGTAAATG
16 1-16 SL2.40ch01 75367587 Solyc01g089900.2 T/G 0.37 GTTTTAAGTATACGATTTGTTTCTGCAATGTACAGGCAACATAGTTCTATGTATTCTGAAGCCTCCAAACTAGAAATGGTCAAGACAACCACAGTCGCTAACACATGTATATGACTGGGAAGTAAGAATTTCATCTACCAATGAACCAAA[T/G]AAGTAAAACATAATAATTCATGATTGATACTCGACTGTCAGACCTAGTATTCATCAGTAACACCAACTATATTGGTTTAAGATCAATAACACAAATATGCAGGAAGTAGATGACTAGATTTCACTTCCATAGCTAGCCTCTTCACACTTC
17 1-17 SL2.40ch01 81793141 Solyc01g099910.2 G/A 0.38 AAAAGCAACAACCGATCCAATGAAATCTCGTCATTAGAGTGCATGCAGACGTTATGTCTGCTCAATCAAATCAAAACGAGTAAGTAATAATTCAAAGAGAAACATTACTAGTAATCATACCTGAAATCTGCACATGTAGTAATCATCTCC[G/A]AGGAAAGCTCTCATAGTAGGAATAGGTTTCATTTTAGGATTCCTTGGCCTAAAAGGCACACTAAGACAAACATATGCCTTGACTAAATCAGGCCTAAACATACACAAGTACCAACCAATCATAGCACCCCAATCATGCGCCACTAAAAAC
18 1-18 SL2.40ch01 85727076 Solyc01g105940.2, Solyc01g105950.2 T/C 0.37 TGCATTGTTTGAGCCTCAACATAGCTACTTTCGAAGATTGATAACAAAAGTCATTGTTTTTATTTCAATCATAGATGACATTTATGATGTTTATGGCACGCTTGATGAGTTGGAACTCTTCACACTTGCTATTCAAAGGTAATTATAATT[T/C]TCTATCGCAAAATAATTACAAAATTTGATTTTCTAGTAAAAATTCAGATGGGATACAAAAGCAATGGACCAAATTCCATACTCTATGAAAGTATGTTATCTTGCACTCATTAATATTATCAATGAAGTTGCGTATGATGTTCTCAAAAAT
19 1-19 SL2.40ch01 90034479 Solyc01g112220.2 C/T 0.35 CATAAACCTCATCAACTCCACTGGCTGCTGAGAATTGGCTATTCCCGGAGACGTTGCTTACCGTGGAGGAGGATGTAGTTGTTGTCGTCTGTGATGATATGCCTACAATTAAATTAATTTTATTATTCGGAAGTAAATCAAATTCTGATT[C/T]ATATTACGAATGGTACCTGAGCTGAAATGGGATGGGCTCTGACTATTAGAACTGAAACAAACTCCCATATCTCTATCTGATGATTATTAAGCAAGTTATTCTTCGGCTGAAAGAGGGATGGGATGAGATTGAGATCTCTTCTCTTCTATT
20 2-1 SL2.40ch02 2309824 Solyc02g005350.2 A/G 0.37 TATCTTACCATATCATGTTGAGGAATCCCCTCTGTTATGCAGACAACTAAGTCCAACTCAGCTTCCATGGCCTCCATAATAGCTGCAGCAGCAAATGGTGGAGGAACATAAACCACTGAAGCATTAGCCTTTGTCTCTACTTTTGCCTCT[A/G]CAACAGTATTAAAAACAGGTAGCCCCAAATGTTCTGTTCCACCCTTCTTGGGGGTCACTCCACCAACCTATTAATGCACACAAAGCATACATGAGGAATTCAGGAAAAAAAACAATTTACTTCTTAAAATATAGTAACAAATAAAATGCA
21 2-2 SL2.40ch02 6352099 Solyc02g014130.1 G/A 0.37 AATTTCTACGTACAAACCGAACTCCCACCGACTCCATACTTGAGCAGATGGCTTAGATCCTTGTTTTCTATCTTCGTAGTTCAGAAAAAATTGCAAACTGCACTGCGAATAACATAAGACCTCTATTCTAGCAAGTAATATGAGCCTTAA[G/A]TGATTACATTTATGGAAGGTAGCCTGTAGATATTTTTTAATGCCTGTCAGTGTATAATAAGTTAAAACACTATGGTAAAAGAGGAATAATGTGTTGGTTGAACTGCGCAATAGCTTCGGAATCAATAGCTCAGTGAGATAGGCCACCCGC
22 2-3 SL2.40ch02 13016181 Solyc02g021240.2 T/G 0.37 TCTTTTCTTTGGTGGAAGTGGGAAGAGGCTTTGGGGTTTCCGAACTTTGTGCCTCTCTCTCCAAATTACTTCTAACGATGAAGTTGAAATTGTTTCCAAATCACTTTACTAACAAGGAAAGAACCTTCTACCCTGTAGGCATAAAGACGG[T/G]GAAATAAAAAGGCAGCTGCTTCAAGTTCTTAAACTTTTAACTCTGAGAAAGAACATGAACAATTTATTATGTTTCTATCACAATAAGAACAATAGTATCTAACGATGTTATTGATATGCACTAAGTACTGAATTGTAGATCAAACTAAAA
23 2-4 SL2.40ch02 16247366 Solyc02g030360.1 A/G 0.37 AGCCTAGCAATGTTTCGCTAGACACTGATATTAACCCGAAGATATCTGATTTTGTCATGGCAAGAACTTTCGGAGGAAATGAGTCTGGAATCATGACAAAAAGAGTTGTTGGGACATAGTAATAATCTTTAAACCAATGAACTATTTGCA[A/G]TATGTGAAAAATCTCCTTATTTTGTTTATCCGACTTATCCTACAGTGGCTACATGTCTCCGGAGTATGCAGAAGAAAGAATATTTTCAGTGAAATCAGATGTATTTAGCTTTGGAGTTCTAGTATTGGAGATTTTAAGCAGGAAGACAAA
24 2-5 SL2.40ch02 21197215 Solyc02g036290.2, Solyc02g036300.2 G/A 0.37 ATGTCATATTGTTCAAGTAAGAACTAAATATTTAATTATCAAACCTAAAAGCACCATAGAATTCAGCCAGATCAATTTAACAAGTAAAGTTGTAAACTGAAGTGCTGTAGAAGTTGAGGGCTGGGTCCTTGTTCAAATAGAACAAGCGAA[G/A]CACCATCACAGAAAATTATTTATATTCAGTAAATTAGTAATTCCATTAAAAATGTATTCTCCTCATAGAGATTCAAAACTTGCATCTCAAGGAGAGAGAATTGGTACAACTACTTTAAAGAGTTCTACAAAAGCATCAACTTCTCAACAT
25 2-6 SL2.40ch02 27338193 Solyc02g055540.1 A/G 0.37 CCCTTTTACCAACAAATAAAAACGAAAGAACAAAGACTGAATGCTCTTGGAGATACTTAAATGTCCGCCCCTTTTATTGAGAAACATTTTTTCAGCTGGACTGAAACAAAAAAACTTTCAACAATCAACAAAATATAGAACCGAGAATCA[A/G]AGCACTCAACGTTGTAAAGAACAACCTAACAGCATTATAAGTCGGACTTCTCCAATACACAAGATTTTGCTTCCAGAGACAGATCCTAAACTGAGAAAATGCACCTTGACTGTATATGCTGGTGAACTTTAGAGGTTCTGAGTTCTCAGG
26 2-7 SL2.40ch02 34191508 Solyc02g069780.2 A/T 0.38 AAAAACAGAGTTGGTTACATATTTGTTGAATTATTCAAAAACAGAAATTGAGCTGCGTAAGTTCTGGCTTCTCCTTTTGTTGGTTAACATGGATGATACTCATGATAGATCTCACTCTCACTCTCTCTCTCTCACACACACAAAAAAAAA[A/T]AAAATTAACACTTGCTAGCCATCTTGTTGAAAAGGCATTACCTGGACTCTGAATAAGGCAAATAGTAGCTGCATTGTTAGTACCTCTATTTTTATTTTGCTTGTTTTTTATTTGATACAGCAGGGGTGTGAGGCAGTGAAAACAAGGATT
27 2-8 SL2.40ch02 35258635 Solyc02g071190.2 A/G 0.38 CAACTATTTGTTGTTGCGAGGACTAATAAACCTCTATTATGGCCAGTGTAATTAGCAGTCAAAGGGAAAACGGTTAGCATTAGTAATGATTAAAGTATTCATTTGTTCCGAAGAGGGGCACACTTAAGAACAGAGGAGTGGCTGAGTAGG[A/G]GCTCTCAACTCCTTCGTGTTCCCGATCTGCAGCATTTCTGTTGCAGCTCCTTTAAGACTACTTTATTTCTAAACACGTGTTCCTAGCAATTGCTACAACCATTGCAGTAGCTATTTTTGAGTAGTGTTGAGTTGTGCGGAGATGCATACG
28 2-9 SL2.40ch02 40093278 Solyc02g081640.2 C/T 0.37 GCACTAGTACCAGGTTATATACTGTGCTCCTTCTAAATTTCTTATTGTTATTCAGGGCAGAGTAGAAGATTCCAACCCAAGCCCAAGTTACAAGTATACAGAGAGAGATATGATACAATCATTCCTGATTTAGATGTAGGAGAGTCTGTG[C/T]CATGTCTGCCAGATTCACAATCCGTTCATTCTGAAATGGATTTTGTGAATTCTGAGAAGGGACATGGATTACCTGCTGCTGATGTCCTTGATTTCTCAGTCAGAGGATTTGATATTATTACAGAATCTGCACATGATTTCCCAGTTCATG
29 2-10 SL2.40ch02 46129269 Solyc02g089840.2 C/T 0.37 ATAAAATAATCCTAACGCCGTTTGTTAACCCCAAATAAACAAAAAAAAAACTACTAGCGTTTCTTCAACGGCAGAAGGACCTATAAATTGGAGCCAATTACAATGCAATAAGCTGCAACTTTTATCAATTCTCTTTATCTCCGAAAGCTT[C/T]AGAGTTAGTTTCTTCGTCTATCAATGGCGTTCAAAAAACTTGTAGCTCAACGTCTTTTCGATTCATACAAAATTAGTCGTCCTTCCCTATCAACATGCCGTATTTGTTCCTCAACCATGACCAAATCGATGGCTTCTCCTAATCCTGAAA
30 3-1 SL2.40ch03 38505 Solyc03g005030.2 C/T 0.37 GGAGTATGATGAGCAGTTTTGGACCCAAAAACAAGACGAAATTGAGGGTCTTTGTGCACTGAAACACATGAACCCATTTCTTCTTCAACAATCACAAATTAATCCATAGAATTTAGAGAGAAGAAGAAAGGTGCTGCCTGCTACTATTAC[C/T]CAGAGTGACTGAAAAAACTCTTCAATTGGGACTTGAAAGCGAAGTTAGAATGGCTTTGGTGTGGTCCCACACAAAAAGTTTATATCATACGCACCATGCGTTTCCCCTTCCGTTCCGTTTCTTCCTAAAGATTTTTTCATATTCGAAAAT
31 3-2 SL2.40ch03 6702041 Solyc03g019770.2 A/G 0.37 CTCTTGTGCTACTAGAAAACAGTGAATGAATCATCTCTTTATCACCATTAAATGAATGTTATATGGTGTGTGAGAAGATTATTTTTTGATGTGCAATTATCTGGGAATGGAATTTATATTATCGTACTAATATTCTTTTGATACATGTGC[A/G]GTGCTATTTTCATTAGTATTCTATAATTAAACTGGAGTTTTCTTTTCTATTCAAGCACCGTGCCTTTTTTCCTCAGTGGACTTGATCAGTATAACTTATAGTATTATGTGTATTTGTTCCCAGGGAATAGTATGGGTTCTAGAATCTGAA
32 3-3 SL2.40ch03 10216978 Solyc03g034250.2 A/G 0.37 ACAACCAAAACTAAAATGCAGAGAAGGTGGGAGACTAGATAAGTCCATACCTATGAAGAGAATTACATATCGAAGAATGCGAACTGTTGTTAGTTCTTGCAGAACCCATATTGCAGCAATAAAAATGATAAATCCTGGCAAGTCCACTCT[A/G]TGGGATCAGTGACTAATTACAGAAGCAAATGCATATCAAGTATTGACACTGATCAAGATCCTGAATATACAAACCTATGCAAAGTCCTCGCAGTGTCCACTGTTCAAGAAAGTAAACCATGTAAGATTCTTGAAGTCCTTTTTAAGAAAA
33 3-4 SL2.40ch03 17025485 Solyc03g046540.1 T/C 0.36 AGCACAAAGTCAAAGGCTCTTTTCTCTCCTTACTGTGACACTCACTCTAAAGACCTTGTCCAACATCTCCTAGGTATACCACCCTCATCTCTAGTCAGGAATTACCTTGGCTTCCCTATCCTCAACCATAAGCCTAAAATGAAGGACTTC[T/C]AGTACATCATCAATAATATGAGAAAAAAACTCGCTAGTTGGAAAAAAAATTCATGACCCTTGCTGGTCGCATCACCCTTGCAAAACTATCCTTGACATTATTCTAGCGTATTCTACGTAATATTTTCGACTCCCTAAAAAAATTTGCAAC
34 3-5 SL2.40ch03 23371652 Solyc03g058810.1 A/G 0.37 AAACCATACATATCAAAGATGACTACAGGCTGTCCTGTGTTGGTAGTCTGTCTAGGAGGAGTTTTGAGCACAATGGTGGGTTCTTGGGTAGCATGAATGTGTCTCAACCTTGCAGCCAAGGTCTGAACCACAGTGTAGGGAGCAGGTTCA[A/G]GTATTTTTTACTGTTGGATGTTTTGATGGCTTTTAAGAAGGTTGTTTTCTATCACTTTTTGATGGGGGGGTGGGCTGGATTTTGTTTATCAAAATTGCTAGAGACTTTTGGAAAATCACTATGGTAATTTGTTTGACCTTTATTCCCCCC
35 3-6 SL2.40ch03 27047365 Solyc03g059410.1 A/G 0.35 TTGACATCGGAGACAGATCTCCTCAAGAGATGTCTTGTCGGTTAATTCCAGAGCACACTTCGGGAGATACCAACCCTCAATGATGTAAGAAGGTGGGCTTGCAACTCTTGGAAGTTAGCCATAGGAGTTAGCGTTTTCGCTATTAACAAC[A/G]GACACTTCCTGTTAGAGCTACCCGCAATAGTGGCTGCAGTGCATGTCATGGCAGGAACCATGTATAGTGGCTGCAGAGCACGTCATGGCAGGAACATGGTTTTGGAAGAAGATGCATCTGAACTTAGAGTGGTGGAGCCCAATCACAGGT
36 3-7 SL2.40ch03 33357866 Solyc03g063760.2 T/C 0.37 TGACCAAAACTCATCCTCTGCATTATCTACATCAAAAAGTCCCAATTGTGATCAAATGCCTCTGGATATCCAACTTGCATAGGATCCCTGGTTGGTTACCCTTTTTTTAGAATCAACACATTCATCGGCTATAAACACTGCATCCATGAT[T/C]TGCCTGCACTTGATAAAGGCCATTTGTTGCCTGTTCACAAGCTTATGCATCACCTTCTTTAGAAGAGATTGTTATTCCTTAAACTAAATGATTTTCCTGATTTGGATAGATGACACTATTTTTACTTGCGTTACTTTTCCTTTAATGAGT
37 3-8 SL2.40ch03 36893476 Solyc03g065080.1 T/G 0.36 AACTTCACTGTGAACTTTAACTTCAACGTCAATGTGAACGTCAATGTATACTATGAACATCAATGTAAACTTAAATGTCAACGTGAATGTCAATGTAACTGTAAGCATGAATATTAATATCAATGTAAACATCAACGTGAGCATAAACTT[T/G]TATGTGAACGTCTAAGCGAGAGTGTATGTGAATGAAATTGTCAACATAAACATGGACGTCAACGTGAAGGTCAATGTGATTATCAACGTCTATGTAAACGTTAACATCGACATCAATGTAAATGTAAATGTGAATGTGAACGTTAACATA
38 3-9 SL2.40ch03 42084323 Solyc03g077920.1 A/T 0.34 TTTGCTCTCTCCGCGTCGATCCTCTAATCTATACCACTGAGGTGCCAAAGGGCTGTCAGGTGGAACCCTTGTAGGCACTTCATTCATGTCAAACACCACTTTGCCTAAATAATCATCTCTCTGCACCATCTCTTTGTCTCTTACAAAAAC[A/T]TCGATAACTGAAGACTGAATCTTCTCTTTGGAAAAAGCAAACACTTGCTTCCACTCAGGGTTTACCTTCTTATCGAAGTGCTTTGTTTTGCCTTTATAGTTTCCGAGTTTGACCTCGACATAAGGATCACAACTCCCTGTCACAGGGTTT
39 3-10 SL2.40ch03 49870313 Solyc03g095360.2 A/C 0.38 TTTAACGGGAGAAGTTCTACAAAAGAAAGTCATACAACTCCCTAGGTGTTTTTTGTGCAACTTGACAGGGTTAAGTAATAACCATCTCTTCCTGAATTGTAAGTTTACTGTCCAAATTTGGAACTTGTTTCTCAACATCACAAGCATGAA[A/C]TAGACAAGGCCAGAACATACATCTGATCTGTTGAGTTGTTGGATTAGGTAGGGAGGAAGTAAGAGTCAAAAGAGATGGTGGAAATTAATACCATCATATGTACGGTGGTCAGTCTGGAAAGAAAGAAATGAAAGATGCTTTGAAGATAGA
40 3-11 SL2.40ch03 50117306 Solyc03g095530.1 A/G 0.37 GCTTATGGGAGTCATCAGTCACAACTTCCTCAATCTATTGGATTGCTCAAACAACTACTCCCTAAATATGGTTTGAACAAAATCTGTGTTTTTTATAGCTTATATTCGGTTAGGAGGGCAAATAATTTATTAAAAATATTTTTTCATGTC[A/G]CTTTGAAATTGAATTGTAATTATCACATAGATTGATAGAAATTATGTCACGCCCCAAGCTACCCCCGAGACGCGGACACGGAACCTAGGACCACAAGTGATCTTAAGCTAAACCTGCTGACATGATCATGAGCATTCTAAAAATAATAAA
41 3-12 SL2.40ch03 56203572 Solyc03g111550.2 C/A 0.37 TGAACTTTGATAAATTCATTAATTTTTGGATCATAAAAAGACTGTATTCATTCAAAAAAAATTTAGAGATGGACATGCTAAAGACAACAACAATAGACGTAATGCATAAAAATGTTCTCTTTTAACTTTGATTTCTGTTAATATTTATGT[C/A]CCTTCAATTTTTAATGTGTACAAATAGACACTTAAACTTATATAAAATTGAATAAATAACACATACATCCTACCTGATAATTTATATGAGATATATTCGAACTGATTTGTATTTGTCTAGTTGTTCATGCCGGACCCATATTATATCATA
42 3-13 SL2.40ch03 60042376 Solyc03g116680.2 C/G 0.37 TGAAGCAGGAATAGAAGTCGGGGAGAAACCAGGATACGGGGAAAATCTAGAGTCCCAGATTGACATAGAAATTAGTGGAGCAGATGATATGAATCACGACATGCCAGATATGGTCGCATCAGAGAGAAATGTCGCCGGAGGGGAGACGTT[C/G]AACATTCAGGACCCTGCATTTGTTGAAGGAACTGGTGGTTTCCATGAAGAGCATAGGCCGCTGGAAGAGGAGAAAAATAAGAACAGTACTGGTCTGGTGATGGATAATGGCCAGATTAACCAGCCAGAGCGGGCACATACCCCGCAACAT
43 4-1 SL2.40ch04 794066 Solyc04g007070.2 G/C 0.38 ATGACCACACGTGACACACGTCTTGGTTTCTTTGCCAATAAAGAACCTCATAAGCTGAAATTCTTGGAGACAGAAGAAAGTTTTGAGTTGTTGGTAATGAGAGTTTTTGGCAGGGGCGGGTGTCCAAATGAATTAGTGGTAACTGGGACA[G/C]AAATTGCAAGAAAATGTGGTGGAGTACCACTTGTAGTAGTGGTGATTGCAGGAGCATTAAGAGGTCGTTCGGACAAAAAGGATTGGGAAAGAGTTGAAAAAAGTGTGGTGCAATATCTTGGTGAGCATACCGAAGATAGCTGCCTGAAAT
44 4-2 SL2.40ch04 5040990 Solyc04g014800.2 C/G 0.37 GCCTATCAAAGGTATGTCACATATCAAACTCGGTGTACCTGCTATTGCATTGTATATTTTACACTCTATTGATCTTGAATGCTTTGATATGTACAGTATCTTCGCAGTCCGAACTTGTGAAGTCCCTCCTTGGCACAGAGGTAATTCACT[C/G]TCTCTATTGTTGCTGTGTACTTTGATCTCACCATGGATCATTTAGTTCATGAATGGCAATGAAACACAGCCTACTTTTGCAATTTGATTATTCTGTTGTAATGATGGTTATGATATGAACATAAACTTTGTTTAATCTTTTTCAAGACTA
45 4-3 SL2.40ch04 13845182 Solyc04g024670.1 A/T 0.37 TAGGATAAACATCTCTATAGATTTCTTTGATTGCTTGAGAAATGATCCCCACAACATCATCTGAATTGAAACTGTAATACAAAGTTTAGCAGTTACAAGTTAAAACAAAGACAATTAAATCATATGATAATAAACATACCATATTTTTTT[A/T]AAAAAAAACCTACGTGTAGCCTTCAGGCTCAATGACAAGTCTATTATGAATGTCCCTCATGCCAACTTGTGTATTGTCATGACCAGGTGTATCTGACTGATTTGTGGTAGGAGTGTTAGGCTCAGAACTATTACCTTGAATGCGAAGACT
46 4-4 SL2.40ch04 16721203 Solyc04g024990.1 C/A 0.37 GGCAGCAGTACGCCAAAAATGACTATTGATGAGGCATTCTCTTATTTGTACCAATTTAATTACAAGTATCCAAATCAAAGAGAGAAGTATAGCACATTCCTTGTTGTTATGATGGGCTTTATGATAAAAAGGTTTGCTACTACAATTCGT[C/A]TATTGTCAATCTTCACTTATTTCTATTCTTATTTGATGATATATGTGGAACTAATTTTTAATATTGCTTACTGAATTGATATTGTTGGTGTCATTGAAAAGGGGAAAAATTTATTCAAAGGGCATTCTAGTTTACTCCTTGGTTTAAATC
47 4-5 SL2.40ch04 22716678 Solyc04g025870.2 A/C 0.37 TAATCAATTTGGTAACTATTTTTACCTTTTTATAATGTCCATCTAAAACCCCAATTTTTGGGGTGTGATCATGTGATTATGGGTTGATTTAGTTTATGGGAATGGCTAGTTGTTAGTTTAAGTGTTATTTAAAAGTGAGTTTAATCATTA[A/C]TTGTGGCTTAATTTAAGAATTGTAGTTACAAATGCAGTTCTATTTTTGTGTTCTTGTCTTGCTCTCGAGAGAGGTTTTAGAACTAAGGTTTTTGATTAATGGTGTGTGGGTATTGGGGCCGAACTGGGTTTAGCTCGAGAGGGTGAATCC
48 4-6 SL2.40ch04 26603172 Solyc04g026350.2 G/C 0.37 GTTCTATTGCAGGCATCAAGTAGATGCATAATAGTTACAAAAGCTTAGTAGCCAAAGGTCAGCTCTCCTTTACATTGTTAATTGAGAATCAGGGAGCAGACATAGTTCAGAAATGAATCAGGGTAATCTGGAGGGGATAAATAAACCCCT[G/C]TAGATTTTAAAGATTGGTAGGGAGTTAGCTTTTAATGATTTGGTAGGTAGTCCCTTCTTCATTTAACAATCCATTCGTAGTATAAATTTTTTGATTACCAAGAAATTCCCGAGTACCAGCGATGCACGGTTTGGAAGTTTAGATAATGGG
49 4-7 SL2.40ch04 31000363 Solyc04g040110.2 C/A 0.37 TGTTTCGAATCCGCTGGTTCAAATTGGTTGTCAATAGAATAGTGAAGTAGCTAGTGACTAATCTTTTCAAATGTATTCTGTTGTCTTTTTAAAATGTATTTTGATGCAGAAAAGAGCACAACATGTACTACACTTGCGCTTTCTAAATAT[C/A]TAACTTAGCTTCAAAATGAGTATTATATATTTAGCTCTAGTAAATTGGATAAGCAAAAAGTGACTAGTAAACTGGGACAATGGGGTGTGTCAGTATTTCTATAATGGCGATGTTCGGGCTAGCTTGGGCACCTAGACTATTGCACAGTGG
50 4-8 SL2.40ch04 36140519 Solyc04g047750.2 C/T 0.36 ATGGACTGAAAGTAGTGGTATTATTCCAATTTGTCACAGTGTATGACTATAGAATTCTGCTGTTTGTCCAATGAACTGATGACGCATCAAGCAAAAGCAAGTCAAATCTACGAATAACAATCATCTTTGGGGCTTAAAAATCATTTTCTC[C/T]TGTTTGTTTTAGGAGAAAAACTTGTGTGAAGATTGCATTTTAAAAGTTTTCCCTATGATGTTTGTTTGTCAATTCTGAATCCTGTTGTGTGCATTCATCGAGTCTTCCATTCCACGAGTTCTAGTGACCTGCAATTTTACTTATTAACCA
51 4-9 SL2.40ch04 40973013 Solyc04g049400.2 C/T 0.37 CATGCCATCATACATCAAGGAAGATGTACGATCAGTGCTTGGCCTACCAGAGCTGACGAATGATAAATCAGTATCCGCTTCAGAAAGGTCAGAAAAGATCCTTGAAGGGATGACTCTTCGTCTATCAAACGGGGACCTGCTTCCAAAACT[C/T]GCTGGCTTTAGTGTCTATACATTATATATAATCAAGCAGAACATCTTATTCTTTTGTAGCAATAGCAATAGCCAGAAACATGTTCAGATAGGTGTAGCAATGGCAATAACAGGTCTTTAAACTTACCCAACTTTTCTGCTGTCATCAGAT
52 4-10 SL2.40ch04 46600361 Solyc04g050520.2 C/T 0.37 GTGAGAAGATCATTTAATTTTCAACAGTTCAAATCATAAGCATAGTCTTACTATTTCATTTCCTCTAATATTTCTGTTCCAATGCTACTTGGGTATTCCAAATAAGATAAGTCAAACAATGAAAACCGTGATCTATTTCTTTTATATGGG[C/T]GCACGGATATAAGAACATTTTCTGGATTATTTTCCAGTGTTCAAATGTGTACAAAGTGACTTGAGATAGTCAAGGTGTAATTTAGACTGAAAGACAATATTTTGATGATACTACTTATTAGTTGAAGCAAAGTGCAACAAAGTAAGAGTA
53 4-11 SL2.40ch04 54245561 Solyc04g057970.2 T/C 0.37 AACTAAAAGTATATTTTGTTTCAATTTCTCAGCATCTAACTCGGCAAGATGCATTCCAGCCTTGGGAGGATGCAGAATGTCTCCAGACTATGCCTGGATCTTCCTAGAAGCTGTTGCTTTTTCCCATTCTCCAATGTCAATGTGTGACAT[T/C]AAAACAAGCAATGGAAGAAACCAACAACAATTATTATGGCTTAACCCTAAATAGGTTAGGGTCGGCTATATGAATACTCACTGACCAAATTCCTTATATAAGCTCAACTCGTGTCGTATCATTGTACCGTTAGTAGAAGCAACCAAAAGA
54 4-12 SL2.40ch04 56138629 Solyc04g071570.2 T/C 0.37 GATAGGTGGAAGTGAAGCATGTGGTCCGAAGATAATCCTTAGAAGTATAAAGGGCATCCAAAGAAGTGAAGAAGTGTTGATTTCATACACTGACTTGTTACAGCCTAAGGTACAGTTGTTCTCAATCTTTTGAAGGACATGACACAATTT[T/C]ATATTATCAAGGACATCACTATAGATAGGAAAGAGTGGATGTCGTGTATTAGGGTAGAAGGCTAGTAGAAGAGAGTGTCGCCTTTCCTTGCATAGGTTTAGTGCCTGTCATAGGACTAGTTGTACCCTTGTAGTTCTTGTGGTATGATGT
55 4-13 SL2.40ch04 60042054 Solyc04g077500.2 T/C 0.37 GTTCAAGACAGAAGAAATTTATCAAGAATGGCTAGGAATCAAGTGTGTTGTTGCTGTTTGATATTATTGGTCAGCAACTACTGACTGAGAGAGTATTAGAATTCCAACTTTTAGCTCTTGAGTATTGACAATTTTTAGCTGGGAAGATTT[T/C]GGTTTCAACGAATGGCAGTAAGAGCCACGCGCAAAATGGAAAAAAATCAACCCACATACCCGCTCCGCCCCGCAAAAACTCTTATCCGCACTGCCACGCAAATACTATAACCCGCCTTGCTCCACAACCGCCCTGCCCATGCCATCCCTG
56 5-1 SL2.40ch05 2142284 Solyc05g007610.2 C/T 0.38 TACCATTAGATCCAATGTATGGGAAGTGAACGATGAGCAGTTCCTTCGACTCAGAATCTTGAAACTAGAAAATCCTTCTTTTTCAAGATGGTATGCCTCAGATGATGCCTTTCCATGCCTTGAACGCTTGGAACTGAAAAGATGCCAGCA[C/T]CTCAAGAACATCCCTTATTGTTTTGAGCATTCTTCGTCCCTGAAATCGCTTAAGATTATATCCTGCAATGACATGCTTGCCAATTCAGTCATGCAAACCAAAGAATCATCAAAGTATTTGCACGGAGCTTCTGGTTTCGAGGTCTTCATC
57 5-2 SL2.40ch05 9039075 Solyc05g014830.1 A/G 0.38 ACTTTAGAGAGATAAAAACTGAAACTATTATACACTTCAAGAAATGAACATATAAGAATAAATAACAATAGAACTAATAAATAATATGATAACTGAAGAAAATTAAACAATACTTAATATAAGATCCAAGTATGTTGAGTTTTTATTCTA[A/G]GCCACATAAATATAGAACTATAAAGTTTTATATGAATAAAGGAAATTAATAAAAGCAAACACCGTGATGCTAGTGATGAAATATTCAGTCTAATCCTTCCTTCTGAGCAGCAACAACAGCCTTTGAAGCGTTACTCTTGTTGTTAGGCTG
58 5-3 SL2.40ch05 10091032 Solyc05g015220.2 A/C 0.37 GTTGAACTTTGGAAAAGTATACGCCTAGACCCAGATGGGCTTAAACGGATCGATATGGACAGTGAGAACACATAGACCCACATGGGCTTAAAAGAATCTAAAGATTTCCACTTATCAACAAAAATATCTAAAGATTTCTTTTTTATGCCA[A/C]TCATACTCTAATGAACTCAGCATAAACCTGTCAGCCTGCTGGCCCGTTCTTCAACTCTTGAATCACGAGTACTGGTTCCAGAAAGAAAAACAATGTCTGCTTTGAAAGGAATCCTAGCAGAAATCTGGATTAAAAAAACATACTTAACAA
59 5-4 SL2.40ch05 15933251 Solyc05g016350.1 T/G 0.36 TTTCGATCGATCTCCAGTCAGATTCGAAAGAATATGAGTACTAAGGTTTGTTCGCCCATGGATGGGGAATCTTTAAGTCCAATCTCGAGTCAAATAGACGGCGAAGTCACCGGCTGCGAAAATTCCGGTGACCCTACATTTGCTATCGCC[T/G]GAATTAATGGTGTCTCAGTAGAAAAGAAGGACTCTCACGGTCAAAGCACTCACGTAACAACACAACCTCATCAAACAGATAGAGAAATCAATAATAACAACATCACTTCTTCTCAAACAGATCACAAATTTGATGAACAACAAGAGAATC
60 5-5 SL2.40ch05 20443535 Solyc05g018320.2 A/G 0.37 AGCTGCACCGCGGGAATTATCCTTCTCCAATGTTGGCAGCTGTATGGAAGAGTAGCCAAACATCAATGGCAAATTAGTAGGTATGACAGGCTGGGCTCCAGGTCTATCTCCACTAGTGGTAGCTATTACTTGCATCATGCCAGGAACTGC[A/G]TAACCAGTCGTACTTGCTGTATTACCAGGGTTAGAATCTCTCAGAGGCATAGAGTATGGTAGGTTCATGATGTTTATTGATTGGCCTTGGAATGGGGTACTGTATGGCGTATTACCGAACTTAACATCCTTTTCAGAAGAAATGGTAAAC
61 5-6 SL2.40ch05 32946515 Solyc05g025510.2 A/G 0.36 TTCAGATCTATACTCAGATTCAAATATCCAATTCTTAATAAACATCTTAATATTCAGATGTGTATTGATAATCAAACATCTTAGTCTTAATAAAAACAAATGAGGCTTAACACTTCCAAGAAAGCTATTATGTAAAGAACGAGGTACTCA[A/G]CCATAGAAATGTCATCATAAGGTCATGTTGTACCATTATTGAAATGTGAACCTTATTTTGCATCGTGAATTAGTTGGATGGAACAAACATTGATAAGCTATTCCTCTTAGAAGATAAAACCTTAAGATTAAGGTCCTCGAGACCTGGACT
62 5-7 SL2.40ch05 39397925 Solyc05g026020.1 A/T 0.37 GTGGTGGTGTTGTTGGTGGTGTTGTTGTTGGTGGTGGTGGTGTTGGTGGTGGTGTTGTTGGTGGTGTTGTTGTTGGTGGTTGTGGTGTTGCTATTGTTATTACTATTATTATTGTAGTTGTTGTTGTTGTTGCTATTGTTGTTGTTGTTG[A/T]TGATGTTGATGTTGATGTTGTTGGTGGTGGTGGTGTTGTTGTTATAGTTGTTGTTGTTGTTACTTTTGTTGGTGGTGGTGGTGTTGGTATTGTTGTTGTTGTTGTTAATGGTGTTGTTGTTGTGTTTTTATTGGTGGGGGTGTTGTTGTT
63 5-8 SL2.40ch05 40920627 Solyc05g026350.1 A/T 0.37 TACTTTTACTTACCTCATTCAAAAGTTCATCAAGGGCTGATAATGTCGAGGATTGAATAAAATTAATTTTATGCTCAATGTTAGCCTTTTTAATAATTGGCAATCCTATCTCGTATGCATCTCGATCAAGGTCTATAGCTATAATCTGCA[A/T]AATATTTGCACACAGAGATCAATGATGTTCTACACAGAGAATATATTATACATTAAACCAATTTTAGTTGTATAAAATTGTGATGCACTAGCCTTGCCATCATCAGGAATTGTAAGGGCAATAAGGACTAAGGAATATCCAGTGAACACT
64 5-9 SL2.40ch05 49143515 Solyc05g041170.1 T/C 0.36 ATAATATATCAATGTATCAAAAAGGTTTAGCCACATAACAAGCATTCAATTAACAATAAAGATCTAAAAGTATTTTATACCTCCATAATTATTCCCCTCATGTTCTCAGAATTATTTGGAACAGCTTTACCCACTCGCACCCTGAAGTCA[T/C]CAAGTTGATACTGAAATCCCTATATAGGTAAAATATTTATATGAGTATTATGAAATTGTGCTTTGTAAGTTCTTTTCTAGAATTTTTAAACAAAAGGAAAAGCAGTGCAAATAGTGTTAAGCATAAAACAAATGTCTTTGGCAAATCATG
65 5-10 SL2.40ch05 50461117 Solyc05g041360.1 A/C 0.37 ATTGTTTATATGGATCCTTCTTGTGTTAAAGTACATAGAAAAATGACATTGCCAAAAAATTGACAAGGTAAAATTTCCATTTATTCATCCAAGGAAACGTCCTTTTTGAAGCTGGAATTGATTTTCCTTCATACCTAACATGATGCATGA[A/C]AGGATCCTTGAATAACCATAGGGTAACCTGAAAATCCTTAGCAAAGACTTCTACAAGACGTTCTATTGTTCCATAGAAATATATTTGTTCAAGAAGGGCTCCAAAAGATGTTGATCGTAAATGAGAAGATTGGTTCTGTAGAAAAACGAA
66 5-11 SL2.40ch05 55044910 Solyc05g043300.1 A/G 0.37 CTCCTTCACGCGCTAATGGGGACAACCCATAAATTGCTCGACAATTCCAGCAGCAATGAAGAGCCTCGAAAACGTGTGAAGCAGCAAACTTTGAAAATCCCACGTGACTAGTATCCTTCCAGGCGGGTACGATTTGGAATAGCCTTTTCC[A/G]TGATTTTAGCCCGTTGTTCTCTGCGCCAGTCCTACGAGATTCATCCTTATTTGTCCTTGCAGTCATAAGATAAATTCATCCCTAGTTTTATATGGAGCATCCACCTCACCTCCCTCCTGTTAGGATTTAACGAATTTTGGGCATCAAATC
67 5-12 SL2.40ch05 62993359 Solyc05g053800.2 A/C 0.38 TAGCTCGACTTATGTACGTATCGAAAAACTAGTGATTCACCATTTGATAGGAACTGGATAGGAGAATCTCATTGTTCTATCTTTTCTTGTGTAAAATGTATTCTGTAAATTAGTGTTCATCTCATCTTAAATTATATATGCTGAACCACT[A/C]TTTGTAAAATCTTCGACTCTGCATTTCCCTTGGCATTTGAGGGGGAGTTTTGCAAGACACAACTGAAATGCATTTGATAAGACCCCCTTGGTGGGAGGTCGTTTCTGATAGACCCTCGACACTGCTTGAGCTAATGTTTACAAAGTATTT
68 6-1 SL2.40ch06 1661847 Solyc06g007650.1 T/C 0.38 AAGAAGGTCATAAGTTCAAGCTACCTCTGGTAGAAATGTAAGAGTTTGTACAATAGATCAAATGTGATCTTATATATCCTCTCACAAACCCGTGCATAGTGGGAGTTTATTGCACCGAAAAACTATTTATTTTTTACTCTCTCCGTTTCA[T/C]TTTATGTGAGGTAGTTTGACTCGGCACTAAGTTTAAGAAGGAAAATAAGATTTTTAAAACTTGTGGTCCAAAATGAATGATAGAAATTTTTGTGATGGTAAATTATTTTATTAAGGCTAAAATAGATATTTTTACAGTGAAATTCTTGCT
69 6-2 SL2.40ch06 6487651 Solyc06g011330.1 T/A 0.37 ATCACAAAAATAATAGGAAAATTGGTTTTTCATAATATCTTTAAATATTATGATAAGATTTCTAAGTAGTAAGATGTCTTCTAAAGGCATTTTACATTAAGCTTTGTTTTTTCAGAATAGGTGAAATGCTTATAGTTTAAACTAGATAAT[T/A]GTAGGAAAGAACTATTTCCAATTTTAAATTTTATGAAAGATTTATGCAGATAGGTTTCCAATTAACATATTGAATTACTTAGGTGTTGAATGAAAAGAAACTAAGTCTAAAAACTAAGCAGAATTGCCACTTTTTGCACCCCTTGGTTGT
70 6-3 SL2.40ch06 13917465 Solyc06g019130.1 A/G 0.36 GTTGATGTTTATCTGTACGTTTATGTTCATAGTAACATTAATGGTCACGTTAACATTGACGTTCATGTATATATTGACGTTCACGTTTATGTTTATTTTTATGTTTATGTTCATTTTTATGTTGACGTTGACATTGATATGTACGTTCAC[A/G]TTGACATTCACGATGATGTTAACATTTACATTGACGTTCACGTTGATGTTTACATTATCGTTCAAGATGACGATTAAGTTGATGTTGAAGTCGAAATCGCCATTGAGTTCACATAAACATTTCAATTGACGTTCACATTGATGTTCAAGT
71 6-4 SL2.40ch06 16073739 Solyc06g030550.1 G/C 0.36 CTAATGAGAGATTCTTTTTGTTTTCTTTAGGATATCTATGGTATGTAACAGTGACATACTTCTTTTAGTTAAGTCTTTCCATATTAAGAGAATAAGTTGTTGCTTATGTTTTTTTTACTTGTTTATGTTGCGCGTGAAATCAAATTGGGT[G/C]AAAACTTGGACTCCTTGTTGGCATTTTTGAGTTTATTAAAGACTCATCATCTGTTGAGTCATACTTCACCAATAACAGCAACAACTGTGAGGCTGGAAATTGAAAATAACAAGTGTCACAGAGCTGAAAGCTACCTATATACCCTCTTTA
72 6-5 SL2.40ch06 22128544 Solyc06g036060.2 A/G 0.38 TTATGATCTATTAATTGTGTGTAGATGCCAGTGGCAAAGCTCAACACAGGTGGTACTCCAGATGCAAAGAAAAAAGTAGATCAGGTGAACAATTCGCTTGATTCTTTCATTAGGCAAGCTATGGGAAAGAAACCCCTACCAAGAACGGGG[A/G]TAGGTCCTGTTCAGTGGACGCAGTGGCTTCATTCTATTGATCAGCCAGGTAACAAATTTAGAACTTGGTGTTTATAGAATACAACATGTCTTTTTTTAATGATTTAACCATCCAGTATCTAAAGTCCATTGCCATAGTATGCTAGTGTTT
73 6-6 SL2.40ch06 28410513 Solyc06g048890.2 G/C 0.37 CTTCGTCTAAGCCTTTGAACGTGAAAAGAAGTGGAGCACTTGATGACAATGACCCCTGGGCTGCTATTGCAGCTCCTGTGCCAACTTCCTCAGCAAGACCTTCAATTGGGCGAAGCCGAGGAACCAAACCCGCAGCTCCTAAATTGGGTG[G/C]GCAAAGAGTAAACCGAACATCATCTGGAATGTAAATTACAAAAGCAAACTAAGGCTCCGCAGCAGATTTGAGTTGATCCTGTAAATTTTTTCCCCGAGGCGGATAAAGTTCCAACTGAAATATGTAAATGGAAGTTCTATAGATGTGTTG
74 6-7 SL2.40ch06 30775982 Solyc06g051140.2 A/G 0.37 TTAAGGCTGATAAATGGAGTTATTCTTTTTTAACACTTGTCCTTTTATTTGTTAAAAATAAAACTTAACTTATACAGACAAATATTTCTAATTTGGATTATAACATAGTATATTTGAAAGAAAAAAATTAATATTGCTTATGATGCAAAT[A/G]AAGGTTGCTTTCAATGTCCCGTGCGATATGCAGAATATCAGCTAATTTGGTGCCTCCAATCAATACATTCATTCACTGACAAATACTCACTCACCCCACATTCTTGCTCTGCACCTTACTGGACTTAATCCTTACCCTTTACCTGACCCC
75 6-8 SL2.40ch06 35268103 Solyc06g060860.1 C/G 0.37 TGAGGTTCCACACTGCCACTACATCCATTCGTACACACTTTGTCATCTAATTTTTTCTCATGATCTGGAAGTATGAAATCTTGAATAGTATCCACTTTTGGAGATTCATAAACATAGCTAGAGAACCAGTTCCGGATGTCTGGAGGCTCT[C/G]ATAAAAATCATATAGGGTAACAAAGATTAGCATTAACAAAAACTATCATTGCCTGCTTAAAATGGAAACTTTTGTATTAGGAGGTGAAATGATCACACTAACCTGAAGCAAGTGACATATCAGCTGAAGATTCCACATCCTAAAGACACG
76 6-9 SL2.40ch06 41603822 Solyc06g073380.2 A/C 0.38 AAGCAAAGCTGTAATCTAAGCAAATGCTAAAGGCACAAACTTGCCATTTTAAGCAAATCACCAAGAAATGTAACACAAGATCAAAGATAAAGATTTCAAGCAACACTATTAACAGCATATATGATGCACTAAGACTCTATTCAGTGCCAG[A/C]ATTACCCCCATTAATAAACAAGTTATTTCAGAATGCTTTGGGGCTTTATGGAAAAGCTTCAAAACTGTTATGTTTGGCAAGACACATTGTGGGTAAGTTATGTGCATATCATGTGCATAAATCTTTTGATGAAATGAGCTACAATTTAAA
77 6-10 SL2.40ch06 45229159 Solyc06g083570.2 C/T 0.37 TCTGATGATTAACTTTTTTACAAGTCATGAATGTGGAGTCATTTCTAACTGCTCGACCGTCACTATTTGTAAGCAGGCAATATTTAGAGGGGCCATGAAAGGCAAGCTCATAGTGACTTGTCCCCTTCCTCCAGAGAGAATACCAAAGTT[C/T]CAATTGCTGTACAAAGATGTTTAGCAGGTATGATTGTTTGTAAATTTAGGTTGACTAGTTCGTAACTTGACATATTTCTCAGCTTGAGTCTAAATTATAAAAAGTTTAACGTGTTGTCGGTATGACCAAGAAATATTTTTCACGAGATAA
78 7-1 SL2.40ch07 396822 Solyc07g005500.2 G/A 0.37 TTATACCCACTTTAACTAATTCCATGGAATACCTACCATCTCCCACCATGATAAACATGGTGCCCGAGCCAACTCGTGCACACCTCAACTAACTCTACGTCATACGTGCCACCTCCTTTCAGCAACATGTACCAGGTGGACAAAACTTAA[G/A]CGCACCTCAACTAATTACACGGAATACCTACCACCTACCACCATGATAACCATGGTGCCCTTCTACGTGATACTAGTCGACTCCCACCAACAATATGTACCATGTGACTCTATCCACCAACGCTATCACCAACGCTAGAAATAGACTAAA
79 7-2 SL2.40ch07 8272212 Solyc07g017890.1 T/C 0.37 TCTTTAAACCAAACTCAACACGGACTTCTTTAAAGCTTTTGTCGCTGGAATAAAACCTTGTAGCATTAGGACTTCTCTTTGTACTTTGCATTGAAAGGAAACTTCTTACGTGATTTTTGTTATTTAGCTTACAAAAACTGTTTTCCTACT[T/C]TACCAAACCTAGTTGAACTCAACTTGGATTAGGTAATATTTTCTCCAAACACGTTTTCCGATTTAGACTTGGATTCAACTACAACGTGTTAATACTTCCCTGTGCGTGTTGCATGTACATGTTAGTACTACATGTTCTACCGTAACTTTG
80 7-3 SL2.40ch07 12477037 Solyc07g019630.1 C/T 0.32 AAAATATCTTCAACATATGAAAATTAGTACAAATCTTAACTTGGAAGAGCATTTACAAGAACAAGACTCATTGAAAAACTTCCCAAATTGCACGCTGATGTGATACGCAATGAATTTAGAAGTGAAGCTCATGAGAAGATCTTTCACACA[C/T]GTACCTTTGTGTTGGTATATAAATAGCATACGAGGGTACATGACTTGTTAAACAGAAGATGAGCCATGTTTTATACTTAACATGTTCCAGATTCTGTGAATTCCTCCCTTTTTTGGTGCAAAGACTATTAAAACGGGAATATGTTTTATG
81 7-4 SL2.40ch07 17962275 Solyc07g021400.1 A/C 0.34 CATATTTTGTACCTCCGAAGCGACAGTTTGTTCCACTATATCCAATTATACTTTAAGCACAATTGTTACAATATATACTTCCTCGACCACTTTATGTTGCACCCTTTTGGTAATAGACATAAATATCCACACCTTTTTTATTTATGGACA[A/C]TAAGTTCCTTTTATTGTTGTCCACCTAGGTTTTTTTACTTGGTTATTGAAGAACATTGTTATCCTTGAGCTAGTTGCTTTTCTTTTTCGTTTTAAATTCTTTTGTTTGTTGTCATTCTTGTTCTTTGGTTTGATTCAATAACATCAAACA
82 7-5 SL2.40ch07 38873153 Solyc07g032470.1 C/G 0.34 ATCCCAACTCCTTTCTAATCTCTCAGGCCCAATCCCCTATCCCATCTCAATTCTGCAGGCCTAACTTACTAATACCCAACCCACTTTTCCCTATACAGATTCTCTTTTTGCCCCGACCCGTTTCTCTGTCGTTCAAAAAAGATGAGAGCA[C/G]TAGTTGTACAACAGAGAAGCTTCATCACAGAGCGTGTAGCATCACGTGATTCCAAGCAGTTGCGACGTCCATTGAACCAGACAACGCAAAAATGCGTGAGCAACGACACTCTTTCCTCCAATGTCTCCTTGAGAAGAAAATCCTGTAGTT
83 7-6 SL2.40ch07 42769040 Solyc07g038150.1 A/T 0.34 ATCAACAACAACAACAACAACAATAACAACGGAAACAACAACAACAACAATAACAACAACAACAACAACAATAGCAAAAACAGCTGCAACAACAGTAACATCAATAACAACAAAAGCAACAACAATAGAAGCAACAACAATAGCAATTAC[A/T]ACAATAATAACAATAACAACAACAACAATAGCAACAATAGCAAAAACAGCTGCAAAAATAGCAACAACAACTTTAGCGACTACAGCAACAACAATAACAACAATAACAGTAACAACAACAAAAACAATAACAATAACAACAACAAAAAAA
84 7-7 SL2.40ch07 47065263 Solyc07g040790.2 C/T 0.33 CTCTATGTACGAATTTTTGGAGGTCACCAGCATGTCCTTAATGCTGAGGAATACAACATTACCGTTTTTCAAAAAAACTGGCTTGAATTGGATCCTTAAAATTTCTCTTAATGGAGGTTTAGATCCTGAATCAGTAGCAAAGAGCCAAAT[C/T]GGTGAATGGAGTAATCTTGGCTGGAAATTTTATTTTTTTTTGAAGAAGGTAACTTGTATTATGAACAAAAGGAATACTCAGAAAAGTATTCCAGTATACTTGCAACAAAATTACAGAGGGAGAGGTGTCCCTACTAAGCTTCATAAATTC
85 7-8 SL2.40ch07 54696990 Solyc07g043510.2 C/T 0.37 CTCCAGTTAGAGTTCCCAGAAAGAAGCATTGGCTTTTTTTCTAAGCCCATTCACCAGCAATAATACTGCATACTGCAAATTGATAACTCTACAGAGCATGGCATGCACATAACAAAATCCTATGTATAATATTGTTTATACTGCTCAGTT[C/T]GTAGAAGGAAGACATTTATGATTTAATTTTACCCGATTTCAAGATAAATTTAAAAAGTATTCTTTTGGATCTCCCGGGGTACGATTCAAGGCATGCCCGATCTGAGGGCCGCTAAACTCTGAATGTCACGTGTCACCTTTTAATACGTTG
86 7-9 SL2.40ch07 55504839 Solyc07g045070.2 C/T 0.37 TTATGAAATTATGAGGTATGAGTATTTGGAGCAGAAGATCTTTACCTCAACTGAATGCGCTTTTAAACTCAAATACAAGCATTAGGTAGGCTTTCCTATCAATGCTGGAAGACTTTGGCAATAAGAGTTCTGTTAGGTAGTTTGAATATA[C/T]ATATCATACTTGGTTATGGAATGTAAAAGGTTAGCTGTAAGCAATTGTCAACACACATCTTAATGATATTTTTTTCTCGTGCCTATACTTTTTCTTTTGATATAAAGGTAACTATATATTCACAAAAACAAATTCATCATTCAAACAATG
87 7-10 SL2.40ch07 62466270 Solyc07g062550.2 T/A 0.38 AACTAAAACAGAAAAAGAGAAAATCTGGGATATTGAGGAGTTGAAGTTTGAAGAGAATGCCTATGAATTATTGTCATTGTACATTATCTTCAGGTTTTCATGTTACTTCCATGTGATCAGTGCATTATTGCATTCATGTAGGGTCCTTTG[T/A]TAACCAATAATTTGGTAACTATTCATGTACTAATTTCTACATAAGTTACTCAAAAATACAAAATTAACGCAAAGTTCCTTTTTTGCAATTTAGAAATCCGTATAACTAATACCTGTACAAGTTATGAGAAAATTTGTGTATTATCTGATG
88 7-11 SL2.40ch07 65006928 Solyc07g066300.2 T/G 0.37 ACAGAGCTTCTATCCAAGAAGACGGCAATCCACATCACAAAGAGTTTGGCTTGATTCAACCAATGAGCGGAAGGAGATTGTCCAAAGCTGTACACACTCCATGATGTGCGTGCCAGGCATGTTTTGGCATCAGAACTTGCTTAAGTTTTA[T/G]CTCCTCTTTTATCATTGTTTGTCTTGATCCACTCCATATATATTATGATGTTTGTGCTCTTGGTCTGGCAGTATGTCTGATAATTTGTTCCTTTGTTGAACGATCCTTGGAAATGCTTGTGTTTTTCATTCCCGATTCAACTAATCCTTG
89 8-1 SL2.40ch08 4475 Solyc08g005000.2 G/C 0.37 CAGAAAAATGAGCTTGGACATCTATCTATTAATCTACTTGAAAATCTTGGTTTTAACTTAATCATAACAACTACTTGGTATAAAAAAAGGAACTTATTTAAATATGCATCAATATTATGTATTTTATTACTGTAGTGGCCAGACTAACTG[G/C]TGCACACGACTATTCCATATGGTACCTAATACCTCCAATTAGCACGGACACCAAATAACTGGTCACCAGTGTAGGTAAATCAGAAGAAATCACCTAGAGCTTTTTGTCTTGGTTGTAACACATTTTATTGACCTCTAAGTCACACCTAGA
90 8-2 SL2.40ch08 17244322 Solyc08g023560.2 G/T 0.36 AATATTTTACTTTTTGTACTCCTTCACTTCCTCCATTATGGCCCTTGGAATTTCTCCAAACATCTTCTTCTTCAAATTTTGTATTGTCTTGGCAAATGATTCTTTCATCCTCTTGGCATGTGGAGTATATGGATGTTGAACATTCTTTGA[G/T]AGAATGTCCTTCATCCCCCTCTTGTTTTTGAATTCATTGACAGTTTGACTCAAGGTTTGAAGATTCATGATAGAATCTTTATCATAATGTTTCTTGCACTTCTCACAGAGAAAGCCACAACATCTTCTACAAGAGGTAGCCCCTTCATGT
91 8-3 SL2.40ch08 23020495 Solyc08g029200.2 C/T 0.35 AAATGGGATTTTGCCCAAATTTTGGATTTTCAGCCGCTTTGTGGGTGCTATGTTTGGTCAAAAAGGTTCCAATGCACTCTTCTGTGTTGAGTACTATTGTTGTGATCGTCCTAATCCTACTCTCCAGGTTCTCAATTCACCTTATTTCAA[C/T]TTCAAATCAGTTACTGCTTCATCACATTCTATGCTGTACAATAATGGTGAAAATGATGATGTCAAGATTTCACAACTTTTTTGCTTAATTTGATAGTGTAGCAACTTTGAATATTTTGGGGAGTTAGCTCAACTTGAGCAATATAATAAT
92 8-4 SL2.40ch08 35423942 Solyc08g045660.1 G/A 0.35 TTTATACCCGTTTGACAATAAGGGAAATATTTTTGTAATAACCAGCTCGAAGTTGAATTAGGATACGAAAATTCCTTAACATTTCCTTTGCATTTTAGGCCAGTTACAATGGCAAATTCCTTCATTCCAAACGCCAATACATTCCCATTA[G/A]TATGACGAACATGCAGTACATCTTTATTTTCATGTCCCACCTCCAACAACAGCAAACACTTGGTAATCTAACCCTGGAAATTACACTTTGGAATTTCCAAATATGGTCCAAAAATTGTTTTCTTAAACAGTGCAACTCCCTCGTCTTTTA
93 8-5 SL2.40ch08 49982665 Solyc08g063130.2 C/G 0.37 TTCAGTATTCAGAATTCATAAACTCGAAATTCTGATCGATCATTGAATTGTTAGGTGTTGATAGGATGTGATGGTGTGAATTCAATGGTGGCCAAGTGGATGGGCCTTCCAAAACCAATTGATGCAAATCGTTCAGCAATTAGAGGATAT[C/G]TTGAGTATCCAAAAGCCCATGGATTTGAGCCCAAATTTTGTGCTTATTTTGGTGGTGGAGTTCGTATTGGTTTTCTTCCTTGTGATCACAAAAGTTTGTACTGGTTTTGCACTTTTACTCCATCTGATGTAGATTGTAAGTCTTCTTTTT
94 8-6 SL2.40ch08 54824782 Solyc08g068440.2 A/G 0.38 CGGCGGGTGTTGATGTAATGACATTTGGCCAGTACATGAGACCATCAAAGCGTCACATGCCTGTTACTGAATACATAACCCCCGAAGCCTTTGAGAATTATCAAGTTTTCGGCACACAAATGGTTTGTTTACTCCTCCTTTCTTTTTATT[A/G]TTTAAAGTCTTTCTGCATATCATGTTCCTGAGTTTGTATTGGGTAATATAGTATATTGCTTGTGATGGAGATACTATATCACATCTAAAGAAAATATATATCTCAATGTCTAATCTGTTTTAAGGTTTGGATATTGTAAGTTTGCTACTA
95 8-7 SL2.40ch08 55144784 Solyc08g068860.2 A/G 0.38 AATGCTTCTAAGAGGGTAAAAATTTATGTGTACAGAATATTCCCGTCCAATCAACTTATCATAATTAAGCAATTCAATGAGAGAGGCATACGAAAAAACACATTACAAACAAGTATTGATTAACTTGACGCAAACATTCGGCAGGAATAA[A/G]TTTTAACCCTGGGAACAAGCAATACTACGACAAGAAGAAGCATTAAAAACGATTATACAAAGTAATCACGTACAGTTCGTGCCAAATCCAATTGTTTCGCCTTCTAAATCATAAAGCACATTGAATCCACGCTGTTGAACCATACCAATA
96 8-8 SL2.40ch08 61240553 Solyc08g080910.2 A/T 0.38 AATCATTATAACTCCATTTTGAGCTTCAAAACTACAAACAATATCAAGAAAAACTTCCTCGCCATTGTTAAAGGAGGACAAAAGTCTCACATCGATGGATAATGAAATGGGTAGACTTCTTATAACGCTTGGGCAATACTCCTCTTTTTT[A/T]GCTAACTTTTGGGACGTAAGTTAGGCCTAACACCTAATTTTACGTGATAACATAGTTATGAGGTAGGTGTTAAAGAATGACAAAAGGACAATAGTCCTACGTCAATGGTTAATGAGATAGGGCAAAACTTGGCAATTCTCCTCCTCAAAT
97 9-1 SL2.40ch09 39870 Solyc09g005060.1 T/A 0.37 AAGTTTGAGCTCATCATCCAAAGCAAAAAGCTTAAACTCAAATCTATGACCATGATTTGGCAACTTAGGTCCACGCCATCCTGGGACTTTTTCATCATTAATTCCTTCTTTAACATGACCATATTCTTCACCATTACCAGAAAATCCTTC[T/A]GGCAAACCTTTTAAACTTGGTGGAATATTAGTCACAACCCAAATAACCCATGGTACAATAGGTGCATCAATATCTTGTACTAATAGTGACAAACTTTTTGTGTCTTGTGGTAAATTGTACCATTCTAATGGTGGTGACATTTTTCTTTGT
98 9-2 SL2.40ch09 7049998 Solyc09g014840.1 T/A 0.37 AATTCCGTCCTTAAACTTTCTAGCAATAAACTTTGAATTATACAACTTATTTCTGTTTAATTGAATACACTTGTAAATAGGATGATAGTTCTTTACAATAAAATCACCTGAGTCCCTGTCAATCGAAGCATATAACCACTTACACTTAAC[T/A]TTTTTGCACTTCACCCTCACTCTATGTTTTTCATTAGGTCTTAGCTTTAACTGTCTTCTATACTCTACAGCATAATCTGCCACTGCTTTCCTGAACTGATCTGCCCCTTCAAAGACCATTCCAAGCTCAAAAATTGACACTTCACTATCT
99 9-3 SL2.40ch09 17892974 Solyc09g019990.1 T/G 0.37 GGTCAAGTTCGTGAATTTTTCTCAAGTCAGGTCAGGTTCAATTTTTGATTGGGTTTTGGGTCTTGGATTGAGCTCGGGTGTTGGTCGAGTTTGGGTTGTGAGTCAGGTGTCAAGGTCAGGACCGAGTCTCAGATCGAGTGTCGGATCTTT[T/G]TCCAGGGGATGAAACCTAGTCGGGTCTATATATATGCAAAGAAGGAACATAGGTCTGATGATGTGACAATATTTATATTAGAACGGTCATTTGTCTATTTTCTCATATTTTACAAGCATTTTAAAAATTTTAAAACATGTTATTGTTTTT
100 9-4 SL2.40ch09 20328015 Solyc09g030450.2 G/A 0.37 CTGAGTACATTTCTTGGTCTCTATCACCTCGGGTGCTCTATATCCTGCACTCCTTGATGGGATAGTAGGAAAACCCATTAGAGGTGTAAGACCAACATCTGAGATGCATCCACTATTATCTTGGGTGAGAAGCACATTCGATGACTTGAT[G/A]TTGCCATGAATTAATTTCCCACCAGAAACAGCATGGATATGGGCGATACCACTTGCAGCTCCATGGGCAATCCTCAATCTAGATTCCCAATCCAGTGTTCTTCCCAAGTCCCTGTTGCCTATAATAAATAAAACAAGGAGCAATGAAAGA
101 9-5 SL2.40ch09 25053529 Solyc09g031970.2 A/G 0.37 TGTAAGGCATGATGTTGTCTTTCCTATCAGGTTTTTTGGTCATGTTGAAGTCCTCCCTTCTGGCTCGTAAGTACTAAACAAACTATATATGTGGTAAATTATTTAAAGAAACACACTTCTTTTTAAAAAAGTATATTTAAAGAAACAAAC[A/G]TGTCAAATTGAATTTTTTGAAGCCACAATGCTCTCAAATTCAAACTCAATTGTTCATAAAATTTGTCGTAGGCGAAAATGGGTTGGTGGAGAGGTCGTACAGGCTCTTGCATATGATGTGCCCATTCCAGGATACAAAACTAAAAACACC
102 9-6 SL2.40ch09 41674855 Solyc09g055910.2 C/T 0.37 AGACTCAACAAATATGAGGTTCCTTTAACTTTGGCATTTACCAGAACTGTCTCAATCCTGAATCTTCCTGACTGGTCACTGATGTTCTAAAATATACCACCTTTTGTCATTTCGAAATGAATTTTCCAGAAAAAAAAAAGGAAAAATATT[C/T]ACAATTTAGTATGCATATCATTAAGGAGGAACATACCTCCTCAGTGTTGAAAATGTTTTTTTCTAAAAAGACAGGAAAATAATGTGATATTTCAGCATTGATCCATCCGATTCTAACCCCTTTGGCTGCTTTGTAGAGCTAAAAACCTCA
103 9-7 SL2.40ch09 45192194 Solyc09g057540.2 T/G 0.37 TGACAAAATTGAATCCATCACACAAGGTCTTCCAAAAACACGTGAGATGAGATTTGATACTTTTTCATCCTGAAAAAATAAAATTTTAAGTTAATTTACTTAAATAATAGGTACGTATCTAAGTAAACATATTTGAGAAGTGTATAATTT[T/G]CTATTAGTTTATATAATTTCAATTCTTTCAATAAATTAATTGATTGTGAGTTCAAGATTATCTCTCTTGTCAAGGGAGTGCAACTCATATGCACGGGGTGGAAAGAAATCCAAGATATAATTTAACACATTTAACACTAGTGTAAATCTC
104 9-8 SL2.40ch09 50156566 Solyc09g059490.1 A/T 0.37 CGTAGATAATATAAAATCACATCAACATGCTGACAAATTTAAACACAAATGTATAAATACTATAACTACTAATCAATCATTGTTTGTAATTATAAATGCTAAACAACTCTTACCGAATTGTTCAATAACTGTCCTTTGAAAAACAGATTA[A/T]AGAACCAATTCTTATTGTCCACTATGAGAACGCCAAGGTTAATGGCCACAGAAATTTCAGCTAGATTCTTCTTGTAATGGTCTTGATCATGCTTCCTAAAAACAGAATAAAAATATAAACAGTATACCATTCATTAAAAAGATAAAGTAA
105 9-9 SL2.40ch09 59515303 Solyc09g065690.2 C/T 0.38 AGAGAAAATTATATACCTCTATATGCATTACTAGGCTGGGAAGTAGTACAAGTTATAGTTTTATCCATACTACTTGAAGGAAATTGCAGCATCTTATTCTGCCCTGGTCACCAAAGAGGGAGAGATGAAAATAATCATGTATATTCTTTT[C/T]TTGAACTTGAGAAAAATGATGATAATCACGTATATACTATCCAACAATATGGTAAATCCTTCTTTTGCATTAATGTTTAACAATTCAGACGAATTAAAAGTACAACCAAAATTTTAGCTAAGAAAGCTGCCTATGATAATACTACATCAA
106 9-10 SL2.40ch09 62774934 Solyc09g075590.1 G/C 0.38 CCGGAAAATAATGTGGTGGTGGAAAATTATAGTTAGATGATGGTGGTGGCAGTGACGGCGGAGAGAAGATAGGCTGACATGGGTTGTCACAGGAAGAACACATTGTGCAGGATATTCGGTCTGCCGTTGTCGACGTGGCGTGAGTTGATT[G/C]GACATTGCGAAGCAAAATAAGGGTACTGAAGACTAGAAATTGAAAATGCAGGGGAGCCATTTGTATGAAAACAGAGGAGATATGAAACAGGGGAAAATCAGGTTATGCACAAAAAGAAGAGTTGTTTATGGTAGTATACACAATAGAAGA
107 9-11 SL2.40ch09 67166911 Solyc09g097900.2 G/A 0.38 ATTTGAATATTTAGTTACCATAATCTTATTCTATTCATGTATTTATATCATTATACACTTTAAGTAATATACAAGTTATTCTTCATACCATATGAATTGCACGATATCCGAGTCAGGTCAATCCCCATTTGGGCTCCTGGTCTACAATGC[G/A]GTTGGGTTTGGAGGTGAAGGGGGTGTTAGAGTGGATAAAGTCCGACATTGGTTGGGGAATGGCCTGATGGTTTGTTTATATGGCCTTGACAATCTTTACAATTTACTTAACAAACCTTATGAATTAACTTCTTCTTTTTTTGAAATTGGT
108 10-1 SL2.40ch10 842479 Solyc10g006100.2 A/C 0.38 GAATTACAAGTTCTAGTTATAGATTCCTCTATGGGAAAGAATAGAATAAGTAAAGAAAAGACCAGGACATACCCGACGACAATGTAGTTGAGACTTTCCATTTACTCTTCAAGAATGTGCGAGTAGATGGAGATAGTGAAGCTGGAAGCA[A/C]CTTGAAGCGCTCGTAGATGCATAAACCAGCAGCACACTGCAAAGCCATTACATAATTAGGAGACTAATTGCAGTGATACCAAAATATCTAACAATTAAAGTTAGAGAAGTCACATTTCACTGTATTCAATATCACATTGAAATGATTGGA
109 10-2 SL2.40ch10 8411374 Solyc10g018510.1 A/T 0.38 TTAATGACTGACCTAGTTATATTTAGGTATGCTGCACTTTCTAGTTATTGGAGAACTCAAGTACTCTCTTTCAGTACCTATAGTTGGCTATTTGTCTAAAAGGAGTCAAGAACTAATATTTCTTCTTTTCTCTTCAACCCGACACGTCAC[A/T]GTACGCAACAACTAATACTTATATAGTTGGCTATTTTTCTGAAAGGAGTCAGGAACTACTTTCATCTTCTTTTCTCTTCAACTCTACATGTCACATAATTGATACTTAACTAGTTGGGTATTTGTCTAAAAGGAGTCGACAACTAATTTG
110 10-3 SL2.40ch10 21902314 Solyc10g044450.1 A/G 0.37 TTGGTAAAGGATTGTATTCTAGTGCCTATAAATTGGGTCTCAATGTAATAATGTAGAAACAATAACAATTCAATAATATTCTTTTCCTACATTTCTCACAACTTCAGAATTTTCTGATCAATTAAGGTAACTATAAACATGGTGGTCGTC[A/G]TGGTTTGAGGGATGTGGATGACACTTCTATTGGGGAAGGAAAACCCGTGAGAAGGGGAGGCACTTCTGCTTACAATTCTCTTGAGGTGTGTTTTCTTTTTAAATTCTTATTTGTACTTTGATCTTTGATTTTCCCTTGTACATTTACTGA
111 10-4 SL2.40ch10 26225296 Solyc10g045100.1 T/C 0.36 TTGTCGCTATCACTTTCACATATTTCCTCCATCTCTATATCCCTCCACATCGATTCAATGAAACCTGTATATCACAAGTTAACTACATCAGATTAAGTTCTAACACCAATATTTATTTGTTTTTTTGGTTTTCAATTGTATGCTCGAAGT[T/C]CACATTAGAGCTTTCATTAAATTTGAATCCCATTCTACAAAGCAAATTTGAGTGTGCTCCAAAAAAGGATTTTCTTCATATTCAGGGCTCAAACACAAAAGCTTTGGTTAAGAGTGAAGTAAACAGTTTCTACTCAATCTTCTTTGCTTT
112 10-5 SL2.40ch10 31551881 Solyc10g045770.1 A/G 0.34 ACTTCATGTCAAATCAAAATCATAAAAAAACCAAATTGAAAGAAAAAGAGTATCTATTTTTTTATATAAATGAATTAACCTTTTTTAACTTTAAATACACAGGAGTATTTATCAATAATATTCCAATTCATTGAGTACACTTTCTCTTAG[A/G]ATCATGGAACTCTGACGGATAATCGTAGATGTACACAAAAAAACATTAAAGAGTTTTTCGAATAAAATTATTTCATAATGAGACTCAACTACTATCCACAGTAACAGAAACCAGAGCTCACCTTCGAAACAAGATGTCACTATGATCCAA
113 10-6 SL2.40ch10 38482645 Solyc10g047910.1 A/G 0.37 ATGTAGGACCATTATAGCTTATCGAAAGCTTTTTCAAGGTCAATCTTATCATGTTAGCATTTTTCCCTCTTATTTTTTTGAGGTGAGAGATAAATTCCTGGAAAATAATGACATTGTTAGAGGTTCTTCTATTGGAGAGAAAGCTAGCCT[A/G]ATTGGGGCCAATGATATACGGAAGCATGTGCTTAATTCTGTTGAGAATGATCTTGGTGATCAACTTAGGTGGTATTTCAAAACCCGATTGGTTTGAAGTTCTTGATGGAGGTGGCTGGCATATGGGCTTTTAGTGATAAGGCAGAGCATA
114 10-7 SL2.40ch10 42975810 Solyc10g050020.1 C/T 0.38 CAATAATAACAAAAATAACAACAACAAAATCAATAACGACAATAACAACAACGACAACAACGATGAAAGCAACAATAACAACAATGATAAAAATGACGATATCAATAAATACAACAAAAACTACAACAACAACGACAACGATGACAACAA[C/T]GACAACGACATCATCGACAATGACGACAACGATAACAACGACAATGATAACAATAATAACGACAACAACAACAATGACAATAACAATGACGACAACGACAATGATGACAATAACAACAACAACAAAGACAACAACCATAACCACAACAAC
115 10-8 SL2.40ch10 45229538 Solyc10g050440.1 G/T 0.38 AATCCACATTCGCCAGAAAATTGCACAGATATAAAATCAAAATGCATAACCAGCTTCAGAAGTGCTTAAGTAGACACTTAAACTTGTAAAAAAATCGAACAAGTAGGCACAAACATCCTACGTGGCATAATACACGTAAGACATCATATA[G/T]GACCAAACTTGCCACTTAGGACGAATGAGTATACTTGTTTAGTTAAATACAAGCTTAAGTGCACACTCAAAGTTAGAGACATAGATGTCAGTTGACACCAAATTAAATGACACATTCATGTATTATGCCTAATCAAAACTACATTTACTA
116 10-9 SL2.40ch10 50005218 Solyc10g054040.1 G/A 0.37 GCTAGTAGATGCTTGTTGACCAGACAACCACATAAATGACACTTCAATTTATTTTGTTTTGGTGACCGTGGGGTCCAGGCTAGCTTTCGCACACCTCGGCTGATTACACGGGATACCTTTCACCTCCTAACAGCAACACGTCTCAGAACA[G/A]ATAGGAAGAAATCACCTAATGTTTTTGTCCCTGTTGGGAATTGAACCTTAGACCTCATGGTGCTCAATCCACATCATTGACCACTAGGCTACACCTTTGGGAGCATAAATGACACTTCAAATTGACTTGTCTATGAACAAGAACAGAGCT
117 10-10 SL2.40ch10 57057607 Solyc10g074390.1 G/T 0.37 CCAGAAGCACAATGGTCATTAGTGTTACCGCACCACCCGAATTTGCTGCAACATTGTCCCGACGCACAAACTTTGCCTCCGCCCTGTGAACCACAATTCTGTGCTGAGGCAGCACTCAGCAAAACCAGAGAAAATACTAAAGAAAAAGTA[G/T]TGAATTTAGAAGTTTTCCTCATTTTGAATGTGATGGCAAACATGTTGCAAACTGAAATATATTTATAGGCAACTGCTTGTGAGGAATCAGTGATTTTTGTTACAAAATTGATAGTCCGTCCTGTCTTATATTGAATGACAGCTTTACTTT
118 10-11 SL2.40ch10 60273786 Solyc10g079410.1 C/T 0.37 AGAGTTGAGTTCTTCTCCATAGGACCTTCTTCAATTGTAGGTCGAACAATAGCCTTTCGAGTCTTGTTTTGCAAATCAATATCGCGGTTGAGGCGTAGTATTTTTCATTTCATGATGTATTACTTGTACAAGATCAAGAATTGTCTGTCA[C/T]ACTATTTAACACCTTTGATCAAATGGTTTCACCCGCGTAATCCACAAGGGATACTAGTGTTAGTAACACTTCTAGCCTTCTTGTTGAGGCGATATACGTATGTTAAAATCAGGGCTGATATGGTTTATAAGAGGAAATTTTGGAGGAATA
119 11-1 SL2.40ch11 97752 Solyc11g005110.1 T/C 0.37 TTAAATAATAGAAAACACAGGCTCAATATGACATAAAAAGTGAAGAGCTCAAGCAACTAAATTTGCTACCGACAACAATCAAAACTGTGACCAGCTAAGTCTAATACCTTAGATAAGATCCTCTTAGTTCATTTTCTAGCAGCAAAGGAA[T/C]GCAATGAATTACTAGTTACACTCCTCATCAGTTCAAAAGGAGAATGAAAAAGAGACATGTCAAACCAATGATGCACAGGGATGAGGGATCCCAGTCGCAGTAAATGCATGCCTAGTTGGCAACTAAAAAATGGAGAAGCCACACTATGAT
120 11-2 SL2.40ch11 5702292 Solyc11g012910.1 A/G 0.37 GCAATGAGGCTTGCCTTAGAAAGTCATATTAAAGTAGTTGGTTGTAATTTGTCAGCCCTTATGCTATGAAAGAGAAGAGAAGCGACTTGACTTGCCAGTGGCCTCTTAAAAATAGCATTTTCTTGAGAGAATTCCTTCAGAACTTCTGGA[A/G]TTCGCTTTGTGTGTAATTAAATTTCCGAAGGAAATCTATAACTTTAATTATTTAAATTTGGGTAACAAATATCAAATAAATGCTCATGTTCACTTAGATGAATTGGAAAATTGCATTTTAACTTGAATCCAAAAGATTTGGCTCCCCTAT
121 11-3 SL2.40ch11 10006256 Solyc11g020020.1 A/G 0.37 GATAACTCAATCATTTCTAAGACTCGTGGATGAATGGGATATTTCTTTACGAGGTCCCAAAATTTTCCATCACAGCTCCTCGTATTTAAAATCATATTAACACTTCCATTCCATATATCCCGTGACCTATGGGTAAGTTGTTGAATTAGT[A/G]CCGATGGTTCAAGTGGATCGGCAACCAATCGATATGCACTATAACCAGTCATATACGTATCTATTAAACTGAAAAATCCATATGAAAGTAAATTAAATATAATTGTTAGTCCAAAATATAACAACCAAATCAAAGTAATGTACTGTCACC
122 11-4 SL2.40ch11 18356842 Solyc11g030490.1 C/T 0.36 TTAATTTTGGGAAGTTGTTGTAATAATCCACATTTGTTCGATTGGTTCGGTTTGTTGTGATGCTTTCTCATTACTTGAAGGTGTTCCAAGACTGATTAGTTTGCTTCCCATCCTTTCCTTGACCCTGCTCTGAGCTATGCTTGCCTTCCT[C/T]CGCACTCCCTGTCTGCTCAATTCCTTGCCGGAGTGCTCCAATTTTTTGTTTGAGTGTTCTTGCCTTTGCATTTGTAACTTCCCATTCTCTTTACCTGTCTCATCTTGGTTGGTTAGGGTCTGTGGATTACATGCATTCAAGTTGGGAGTT
123 11-5 SL2.40ch11 20046062 Solyc11g030870.1 C/A 0.36 CTGGTGTGTTGTTTGTTCTAGCGATAGGATCCAAGCACGATTCTGATTCTCAATTTTTTTTCTATTCTGGAATGAATATAATCTAAGGAAATACCTGCCTATACTGAAGAGGGGCAGGCTCAAGGCAATGAATGGTGGTATCAGTCGGGG[C/A]AAACACAATACTCATTCCCGCAACCCTTGTTGCAGTTGTTAGAGTCAAATGTAATGGCACGAGAAGTTGTCTTAATCAAAAAACCTTCCGAATGGAATAAGCTACTATAACTTTCTTTGTCGTCAAAGGAAAATGGAATCATACCTTAGT
124 11-6 SL2.40ch11 28367572 Solyc11g040040.1 T/C 0.36 CCTCTAATGAAAGGACTGGATGAGACTGCAACCAAATTTATACATGTTAGCTGCAACAAACATACCTGGTCCGTTCAAATTTGTTTGTCTGATTTTAACTTGACATGAAGTTTAAGAAGGTCAAGAAAATTTTAAATCTTGTAATTAAAC[T/C]AAAGAAATGTATAATATACAAAAATATGCTTTAATGTGGTCTTAAACATGTCATGTGAAAAGTTAGAATGAAAGAGATATCAAAAAGGAAAAGAGATTTTAAACGGAATTAAAAGAAAAGTAAGATACATAAATTGAAACAGACGAAGCA
125 11-7 SL2.40ch11 32179330 Solyc11g042500.1 G/A 0.35 GATTGAGTATGAAAAGGCTGCAACTGGAGCAACAGGTCCACAAAAACTGTCATCGAGCCTCATTTGTATGGACTATCAACTCTAAACTTCTCCAAAATTAGATTAGATTCTAAAATCTGATTCAAGAAAATGCTCTTACCGGTTATGCTC[G/A]CCTCTGCAGCTATTACCCTGAAGTGTGTCGCAGCATCAATTGGTGAAAAAGTCCCATTGACTAAAAAGCTGCCGGCAACAACAACAGTATGATCTTTTCATTTAATAGCCAAGCATTTGATTCCTCTATGGTTTAAGGTTTTTACTCATC
126 11-8 SL2.40ch11 35999963 Solyc11g044650.1 C/A 0.36 CCCTGATAATTGCGTCGTTGTGCTTGGGCTGTGATGTCTATCAGCCACATGGAAAGTTCAATGTGCTCATCGGCGCTTGACCTTGAGATGTGGATCATCCAAGGCACATTAGCATGGCGTACTCCACCTGTTAGAATTAGTGTTTGAAAC[C/A]AAAATCCTCCTCAGGAGGATAGATGTGGCAATTCGGTTGAGATCCAATGTAGGGAGGGAGGACCATCACGGCTCCTCTCTTCTCGAGAATTCATACATCCCTTATCAGCATATGGACACTTATCTCTCGAGCATAGGTTTAGGTTCAACC
127 11-9 SL2.40ch11 43153452 Solyc11g056470.1 T/G 0.37 GAAGCTTCTTTTTATCGCTATAAGGCATAAGTAGTAAGGCAAGTAGTAGACCTATAAAGAGAAAAGGAAGCGCTATCCCTCCTACTTATGATTCAACATTTAGCTCGATCTATTCTAGATGTATTTTTTACATCAATGGCATCAGCTGGA[T/G]CTTGCACCGAATTGCTTACCCGCGGAACTCATTTAACCCGACCCTACATAAGTACTTTCACCTCTAGGAAAAACTTCTGGGATGAGCCCTTCTACTCGTAATTTTTTCATTTTGATAGTAGAGGGAAAATTATCGATCCCAGACCAGGCT
128 11-10 SL2.40ch11 46329248 Solyc11g062310.1 G/A 0.37 TATAAATAGGGTCTTTGTGTAATAATGTGAAACAAACAATTCATAACATCCTCCTATATTTCTCACATGGTATCAGAGTCTCTACAATATTGGTAGAGAATCAAAGAGCTCTCATTGTCACCGGGTGGCTATTACCCATATATGTTGCCC[G/A]TCTAGCGAACAATTATGTTAACAAAAGTAGGTCGATGATATATGCATCAAACATATATTCAGGGGAAGAAAACTTAGTCAAACAGCTCACCCGCACATCAATATTTGGTGACTTCCTAGGGTTGTTATGTGTCAACCCCATCTTTACGGT
129 11-11 SL2.40ch11 52219700 Solyc11g071730.1 A/G 0.38 CTTAATGAACTTGCTGAGGATCTGAAGCAGGATGTTGAAATGGAAGATAAGTCATCAGATATTTTGCATAATCCTATTTCAGAGGACCTAAGTTTGTTGAGGATGAAGCTTGAAGGAGCGCAGGAAAAGCTTCTAAAATCTGCCAATACC[A/G]TAAGTATGTTTGGTTCATTAGAGAGAGCAATTGTTGAGGTTGATGAACTTGCAGAAGAAATTGAAGGACTCGAAAAGAGCATTGAAGTGAAAAAACAAGGATACACATCCTTCAAGCTTCAGTCGTCACAAATGCTTGAAAAGAAAGTTT
130 12-1 SL2.40ch12 620880 Solyc12g006010.1 G/T 0.38 ATTATCTGCCCTTAGTTGAGGTTGTTACCAAGTTGTGATCTGCCTTTGTCTTCATGATACGCCAATAACCTGTGATATACCCACTTCTTGAGCTTTTTGAAATTCACTTTCTATTGCAAGATCACCAATGTTTCTTTTTGACTATACACA[G/T]TTCATTCTGGCTATATAACTTGATATCATACGCAATTTTGTTGATTTTGTTATGTAGTTGATGAAAAAGTTACTGGCGGACCTTTTTCTTTCTTCGTCCTATCTTTTATTTCCATCCCTTACTGTTATTATGAAGGTGGGCAGCTCACTA
131 12-2 SL2.40ch12 5998763 Solyc12g015960.1 T/C 0.38 AAGGACCAAATCATAACAAGAGAAATTGTCCAAAAAATTCTAAACTAAGTCTACACCAACACCCAGTTAAGAGGTACATTCATATTGATTTGTTTTTATAAATTATTTTAGATTACTTATTAAATTTAATGTCTTGTTGTGTTTTTTTTT[T/C]CACTCATATTAGTCCACCACTGGAAAAAAGAGGGGTAGAGGTCATTATGAAAGAGCAAGCACCAGTAAGACTGGTACAAGAAGAGGTGCAGGAAGTGGTTACAGGAAGAGGCCTAAAGTTGTTGGACAAGGTGTATTTGTTGCTGATACT
132 12-3 SL2.40ch12 10263688 Solyc12g019430.1 T/C 0.38 CCGGATGCATCAGCTTGTAATACAGTCACATTCGATAACTCACCAGTCGCTGACACAATACAAACAGCCCTTGGACTACTGTGAATAAATGACAATATTTTTGATGACACATCCTGAAAATCAGATAATAAGGATTTGTAAGATATTGTC[T/C]GTGACTCCCAACCAAGTTAACTTCTACCGGATTTTATGAATTATTATAACAAAACTGATATGGAATAGTTGCAATTCAATAAGAATGTTTGCTGGCTATTCCCAAGTTTCAAATAAAACTAAATGGTTTTCAAGTCAAAGGAAATAGAAA
133 12-4 SL2.40ch12 15129922 Solyc12g021280.1 T/C 0.37 CAAACACTCCTTTCCAACAGACTGCAATCCATCTCCAGATGTCTGATAAAGAGGGAAGTTGTGTTTCTCCCTCATCAAAAGAGCCCACAATGCACCTCTTGAAAAGTCCAGTGTCTCTTGCCCTTGGTGCCTCTGCAAGTTGAAGGTTTC[T/C]TTCACTGTACGAGATCTTAGTCTCACCTGAAGTGTTTGATATCCAGCTTTCTCCAGCTGCTGTGGCATAAGAAAGGTCCTTCTTAAAATGTTTGGGTTGTACTGCATTCTTCAGTTGGTGAGAGCATTGAATGAACCTCTCTATCTTAGA
134 12-5 SL2.40ch12 21053801 Solyc12g027820.1 A/T 0.37 ATCGGAGTGTCACGTTTCGACACATAGTATTAGGGGATCGGAGTGTCACGTTCCGACACATATAGGGGATCGGAGTTGTTTCTATCTACTATTATCTAAAAATAATCAAGTTATTAATGACTGGACGAAACCAAGAAATAACCCCTCACG[A/T]GCGAAATTATAGAAGATCCCCTTTAAGATCAAACAATTCCATCGAATTGAGTATGATTGTATGTGTGATAGCATCTACTATACCAGGAATATCAATGAACCCAATTATTGCAATTGCTCAGGATAGCCTTTTTTAGCTTCTAGGGTCTAT
135 12-6 SL2.40ch12 25725692 Solyc12g035500.1 A/G 0.37 TGCAATCTTTCCCCTACTTATTTTCTTTTTGTAGCAATCCAGATATCACGCTAGCTGAGAGTTGGGCAAATGGTTGGGATATATCTTTCAGGATACGTTTGAATGATTGGGGAAATAGATAGGGTGGCACAATTGTTACATGCAATCAAC[A/G]AGTTCAATGGTTTTGCTGCAAGGACAGACGGTCTCGCGGGTTCACTCTGAGGAAGGAAGGTTCACAATTAACAAGCTCTATAAGAAGGAACTAGGAACTCAATAAAGCCTCAGGTTGGCTAGATTGAAATATGTATGGACAAGCCAAGCA
136 12-7 SL2.40ch12 30202652 Solyc12g036260.1 G/A 0.37 CTTTTATGGTAATAGCAACAGCCGTTACGGCAATATTTGTGCAAATCATGGTGAAATGGCTTTATGATCCATCTAGGAAATATGCTGGATATCAAAGAAGAAACTTAATGAATTCCAACAACAAATTACCCATACTAGTTTGCATCCACA[G/A]CCTTGATAACACTGCTGCTATACTTAGACTGCTGGAGAAATCTAATCCCACTAGGGATTTCCCAATAGTGTCCAATGTTCTCCACCTTATAGAGCTACGCGGTCGAGCATCTTCTGTTTTCATCTCTCACCAAGTTCAGACAAAGGCTAT
137 12-8 SL2.40ch12 35206364 Solyc12g038400.1 T/A 0.37 AAACTGCAGTCGAGTTGTTGAAGCTCATTCACCGATGGCAATTGTGATTCCCAATAGAGTCTCCAGCTTGAGCATAGAATTACGTGATGGTGACATCTCTTGGCCTGACTTTTTAGACAGAGTGAAAGATTCCAAGTAAGAACTCCTGCC[T/A]CAGAGATTCTTATAATTTTTTTGGTACATGTTTAATCTAGGAAAAGGACAATATAGTGCTTGTTCTTTTTTTTGTCATACTTAATTGTTGCCAATGAATTGGGTCAGAGTTTGGAATGTTATATCAACTTCTTCTTTTAAACATTAAGAA
138 12-9 SL2.40ch12 40010179 Solyc12g040610.1 C/T 0.37 CTTTATAGGAGGTTTTGCAATTTTTTTGCGAACATTAGTCGGATAGGATTGAACCCTCTTGTGATCCATTATGAATTCGATTCACGATGGCTTGGAGCCTACTTGAGATTCTGTTTATATTTCGCACATCACCAAAAGTACTCGTCATTA[C/T]AACAAAAAGACCATAAATGGGGAAAAGAAACTTTAACTTAGTCATTTTACTGCACTATGTGCTTCACTTCATCTATCAAAGATTTATGTGTTTCATCCCCCTTAAAGTACATTCAACAACCTTATTATTAGAACTTTTCTAACAGAAAGA
139 12-10 SL2.40ch12 45641599 Solyc12g044840.1 A/T 0.37 CCTTTGGAGTGGTCTGCACGTCAAAAGATAGCTGTTGGAGCTGCACGAGGTTTGCGATATCTTCATGAAGAGTGCAGAGTGGGCTGCATTGTCCATCGGGACATGAGACCAAACAACATTCTCATCACTCATGACTTTGAACCACTAGTA[A/T]GGGCAACCTCTTACTAATAAGAAAGTTATTCAAGATAAACGTTAACTTCTAACCAAGGCTCCTTCAGAGCATAAAAAGAGTCTGCACCAGTGTCTCGTATTTCCCTGTGTATCTATTCTTGATAGCTAATGACTTGTCAAAATTCTCTTA
140 12-11 SL2.40ch12 54012179 Solyc12g062990.1 G/C 0.38 AACAACAACAACAACAAGAACATCGACAGCAACAACAACAACAACAATAACAACAGCAACAACAATAATAACAGAAACAATAATAAAAATAAACAATAACAATAACAACAACGTCAATAACAACGACAAGAAGAAGAAGAAGAAGAAGAA[G/C]AACAACAACAACAACCTCAAATAACCACAACGATGAGTAAGAACAACAACATAAACAACAATAACAAATAATAGCAACAACAACAACAACAACAAAAAGGACATACACAACAATAACAACAAAAAGATCAAAAACAACAACAACACCAAC
141 12-12 SL2.40ch12 55161472 Solyc12g070200.1 C/G 0.37 TTTACCTTCTTTTCTAATTTTATGCCTACTTTTTTTTTTCCTTCACTCTATTTAATATTTTTTACTTTACATAAGTTTAAAAAAGAATTAAAAATTGGCAAATAAGCGTGCAAACACATAAATATATTCTTTAGGCATGTATGAACATAT[C/G]AATGAGTTAAACTTCTTTTAAAAAATCTAAAAATCGTTATAAGATATAAGTAATGAGCAATTGAATATTTGAACGGGAACCTAGAAGCTTATTTGGAATAATTTAATTGTTTATACAATTGTCGTTTCTAATTGTACGCAACCAATTGAA
142 12-13 SL2.40ch12 61036454 Solyc12g082740.1 T/C 0.37 AAAATAGGATAGAGGTTATTGTTCCCTATATGCAACCAGGGTTATCGTGCATTGTGAAGACATAACAAATTAAATAGGGCTTTTACCTATATGCAACTAGGGGTTATCCTTCCCTATGAAGACACGAAAATTAGGATAGGAGTTAATGTG[T/C]CCTATATGCAACTAGGGGTTATTATGTCCCGTGAAGGCATGAAAATACAATTGGGGGGGGGGGGGGGTTATTGTGCCCTGTGAAGGCATGAAAGATAAAATAGGGAATATAGTGCCCTATATGAAACCAGGAGTTATTGTACCTTGTGAA
143 12-14 SL2.40ch12 65136464 Solyc12g099800.1 C/T 0.37 ATAGGAGCACTCAAAGAATTTAATTTTGAAGCAAATAAGACCAGTCTATTGACTCGCACAACTTGTCATTGTCATTGTTAGTAAAATTAATACTACTATTACTCATAAAGCCAGGTGTGATGCAGATAAAGAACAGATTCCACGGTTGCA[C/T]GGGATATTACTAGCTTTCAAGGCAGTTTCTGGCTTGAAAGTGAATCTTCTTAAGAACAACATCACAACATAATTAGTTTTTATGCACCCCCAAGATTGGTGGGCAGCTTCTATTAGCTTCTCCTTATATTTGAAGGCTGACAGACAGGTC
실시예 2. MABC 143개 토마토 염색체상의 위치
최종 선발된 143개 마커의 염색체 상의 위치를 도 2와 같이 나타내었다. 12개의 토마토 염색체를 막대로 나타내었으며 각 막대의 아래에 염색체의 길이(Mb)를 표시하였다. 왼쪽은 염색체의 영역을 나타냈으며 오른쪽에는 선발된 MABC 마커의 이름과 위치를 표시하였다. 아래 그림을 통하여 선발된 MABC 143개 마커가 염색체 전반적으로 분포하고 있음을 확인할 수 있다.
실시예 3. MABC 143개의 토마토 124 개체에서의 다형성 검증 결과
선발된 MABC 1개 마커를 이용하여 토마토 124개 개체에서의 다형성을 조사한 결과를 도 3과 같이 나타내었다. 그림의 가로축과 세로축에는 124개의 개체를 순서대로 나열하였으며 각 개체별로 교배조합을 만들었을 때 선발된 143개의 마커에서 몇 개의 마커가 다형성을 나타내는지를 표시하였다. 다형성을 나타내는 마커의 개수가 많을수록 박스의 색이 진하게 나타나도록 설정하였다. 도 3을 통하여 선발된 143개의 MABC 마커가 유전적으로 다양한 계통으로 이루어진 집단 내에서 전반적으로 높은 다형성을 나타내는 것을 확인할 수 있다.
실시예 4. MABC 143개의 토마토 19개 대표 품종에서의 다형성 검증 결과
124개 토마토 계통 내에서 19개의 대표 품종을 선발하여 교배조합별 다형성을 검증하였다. 19개 계통은 솔라눔 리코퍼시쿰(Solanum lycopersicum) (Group=BIG) 14개의 계통과 리코퍼시쿰 변종 세라시포르메(Solanum lycopersicum var. cerasiforme) (Group=CER) 5개의 계통으로 구성되어있다. 124 개체에서의 다형성 조사 결과와 동일하게 각 계통의 교배조합 내에서 다형성을 나타내는 마커의 개수를 상자의 진하기와 크기로 표시하였다(도 4). 높은 다형성을 가지는 경우 상자의 색이 진하고 상자의 크기 또한 크게 나타난다. 상자의 색과 마커의 개수를 동시에 표기하였으며 도 4를 통해 선발된 마커가 유전적으로 다양한 집단에서뿐만 아니라 같은 그룹 내의 개체 간 조합에서도 높은 다형성을 나타내었다.
실시예 5. 교배조합에 따른 MABC 143개 토마토 염색체상의 위치 예시
주요 육종자원 교배 조합 내 MABC용 SNP 후보를 이용하여 다형성 (polymorphism) 검증을 수행한 결과, 염색체의 영역과 PIC value를 고려하여 선발된 143개의 후보 마커를 이용해 각 조합 내에서의 다형성을 검증하였다(도 5).
도 5는 선발된 143개의 후보 마커를 이용하여 같은 그룹 내에 속하는 개체 간의 조합과 다른 그룹에 속하는 개체 간의 조합 내에서 다형성을 검증한 결과를 나타낸다. MABC 마커 선발에 이용된 124개의 토마토 계통은 솔라늄 리코퍼시쿰(Solanum lycopersicum) (Group=BIG)과 리코퍼시쿰 변종 세라시포르메(Solanum lycopersicum var. cerasiforme) (Group=CER)인 두 가지로 나뉘어지며 같은 그룹에 속하는 계통들은 상대적으로 유전적인 배경이 유사하다. 선발된 143개의 마커가 유전적인 배경이 다양한 계통간의 교배조합뿐만 아니라 유전적인 배경이 유사한 그룹 내 계통 간의 교배 조합을 이용한 육종 과정에서도 활용이 가능한지 확인하기 위하여 같은 그룹 내의 개체 조합 (BIG vs BIG/CER vs CER)과 서로 다른 그룹에 속하는 교배 조합(BIG vs CER)을 이용하여 다형성을 검증하였다. 도 5의 (1)의 경우 BIG 그룹 내의 두 계통을 이용하여 다형성을 검증하였으며, (2)의 경우 CER 그룹 내의 두 계통을 이용하였다. (3)과 (4)는 BIG 그룹과 CER 그룹 내의 계통을 이용하여 다형성을 검증한 예시이다. 12개의 토마토 염색체를 막대로 나타내었으며 각 막대의 아래에 염색체의 길이(Mb)를 표시하였다. 왼쪽은 염색체의 영역을 나타냈으며 오른쪽에는 교배조합 내에서 다형성을 나타낸 후보 MABC 마커의 이름과 위치를 표시하였다. 같은 그룹 내의 교배조합을 이용한 (1) 및 (2)도 염색체의 일부 영역을 제외하고 대부분의 영역에서 후보 마커가 다형성을 나타냄을 확인하였다.
실시예 6. Luna probe를 이용하여 분석파이프라인을 통해 선발된 SNP 마커 정확성 검증
분석파이프라인을 통해 선발된 SNP 마커의 정확성을 검증하기 위하여 Real-time PCR을 이용한 Luna probe(unlabled probe)의 melting curve 분석을 통해 SNP를 확인하였다. MABC 마커 세트와는 별도로 솔라눔 리코퍼시쿰(Solanum lycopersicum) 및 솔라눔 핌피넬리폴리움(Solanum pimpinellifolium)에 속하는 계통간의 교배조합에서 다형성을 나타내는 SNP를 염색체별로 랜덤하게 선발하였다. MABC 마커의 경우 PIC value > 0.3을 기준으로 하여 필터링 과정을 수행하였지만 Luna probe 검증에 사용한 SNP의 경우 다양한 PIC value를 가지는 것으로 선발하였다. SNP 검증에 이용된 18개의 Luna probe 시퀀스는 아래 표 9에 나타내었다.
SNP 검증에 이용된 Luna probe 정보
No. Chr Position Allele PIC Luna probe(unlabelled probe)
Primer sequence(5'-3') Probe sequence
(서열번호)		 Product size
(bp)
Forward
(서열번호)		 Reverse
(서열번호)
1 1 4686399 T/G 0.02 ACT CTG CCT CTG CTG GAT CG(144) GGA TCT TCC TTA TGC CGG ATC(145) TGC ATG ACT AAT CTG TTA AGG AAG AAG GCT T(146) 156
2 1 80404624 T/C 0.02 CTC CCT TCT AGT GTA ATA TCA G(147) CCC TGA ATC TGC AAC TTT CTG(148) TTG CAA ACG GCT TCT TCT GTC TCT GAG AAG C(149) 139
3 2 31384147 C/G 0.02 CCC CTT AAC CAC TTC ACT AGA(150) AAG GGC GGA GCT GAA GGG A(151) AGC CCC AAA AAA TCT CTT TTT GCC TTA TTT G(152) 157
4 2 41129419 G/C 0.37 ATG CAG CCA ATC TAA CTA CGA A(153) GAT GGT TTA TTA TCC TGT TCT GTC(154) TGG TGT TGG ACA AGA GTT AGT GAA CAA CCC T(155) 151
5 3 1578846 A/C 0.38 AAA GTT GTT GGT CCT GTT GCT G(156) CTG CAG CAC ATT AAC AGA ATA C(157) GTG TGG CAC AAC TGC AAT GGT ATG ACT GTG G(158) 154
6 3 60970656 C/T 0.37 GAG AAT CTA AAG AGC CAC TGA A(159) AAA TTG CAC AGT AAA CTT GAG GA(160) ATT ATT GAA TTT TAT CCC AAT ACT GAT AGC(161) 145
7 4 59090349 A/G 0.02 CTC AAA TTT TCG CAG TGC CTC A(162) AGG AAC CAA GAT CTC CTG CTG(163) TAC ATT GTG TAT GTC ATT GTT GTT ATT CTA T(164) 154
8 4 62077725 T/C 0.29 AGG ACC TTG AAT TGC ACC TTG T(165) GGA TCT AAA TGG CAG TTT GGG(166) ATA TAG AAC TAG GTC TTC CGG AGA TGA CTG T(167) 153
9 5 60310090 T/C 0.02 TCA TGG CTT CTG GTA AGT CCT A(168) CAT CCA CAC AAC AAC CTG TCA TA(169) TGA TTT TAT GGG GTT TCG TTA GTA TGT TTC T(170) 152
10 6 40031690 G/T 0.02 CTT TTG TAC ATC ATC CTG CAT G(171) GTT AGC CAC AAT CTA ACA TTA AG(172) ACA AGG ACA TGA GTT GAA AAA GTA TCT TCA G(173) 144
11 6 44630172 A/G 0,02 CAC CTC TGC ATA TTG CTT TGG(174) GGT GGT AAC TGA GAA GGG ATT(175) GTA AAT GCT GCT GGC ATA GAA GAT ACA GCT A(176) 151
12 7 57277111 G/C 0.37 CTT TTG TAC ATC ATC CTG CAT G(177) GTT AGC CAC AAT CTA ACA TTA AG(178) AAA CGG AAT ATA AAA GCC TGT TGC TTT TTC C(179) 150
13 8 53144148 G/A 0.02 GTA GTA CTA ATA ATG AAG AGC TC(180) CAG CAG CAG AAG TAG ATA TTG AA(181) TGT GCG CCA TTT CCA GCA TTT CCA TTG CTG T(182) 152
14 9 154993 A/G 0.37 ATC CCT TGC ACC TTG AGT ATT T(183) ATC TGC ATC CCT AAT GTC TTC A(184) GTA CAA ATA AGC ATT ACG TTT GAT CGT GTG T(185) 152
15 9 55798375 A/G 0.37 AGA CTG TTG CTG CAG CTG GG(186) GTC ACT GAA AAT ATG TGG TCT TA(187) GAG CTT GAT ACC TAT AAA AAT GAA GTT GTA T(188) 151
16 9 67448422 T/C 0.29 TGA TTC CCA GTG GCA AAT GTA C(189) TTC GTA TGA GTT TCC CAT TTG C(190) ACC AAC GAT TGA AGC TAC CGT CTG TTA ACA G(191) 154
17 10 7318167 A/G 0.02 TAG GTT TAG CAG CGA TTT TGT G(192) CAA ACC ACC ACC TGA AAC CC(193) TGG TTG TTT GCA AAA ACT CAC TCA AAA GGG A(194) 156
18 12 3136269 C/T 0.02 GTG GTC AGT CTT ACA CAG AC(195) GAA GCA GCA ACA GAC AAA GTG(196) AGA ACA CAC AAT CTG CCT CTA CAT TTC AGT T(197) 151
Luna probe를 이용한 SNP 검증 결과는 아래 표 10에 나타내었다. 18개의 마커를 이용하여 교배집단의 부모본 2 샘플과 F1 5 샘플에 대하여 genotyping을 수행하였으며 분석결과와 실험결과를 비교하여 일치율을 계산하였다. Real-Time PCR 수행 결과 melting peak가 나타나지 않은 경우'(-)'로 표기하고 일치율 계산에서 제외하였다. 18개의 마커를 이용하여 각각 7개 샘플 총 126의 SNP 정보를 검증한 결과 4개의 SNP를 제외한 121개의 SNP가 분석파이프라인에서 분석한 정보와 실험적으로 검증한 결과가 일치함을 확인하였다(1개의 샘플은 판독불가).
도 6은 MABC 마커 선발 과정의 정확도를 검증하기 위하여 Luna probe를 이용하여 SNP 검증을 수행하였다. 마커 선발과 별도로 토마토 유전체 내 18개의 마커를 선발하여 부모본 2 샘플과 F1 5 샘플에 대하여 genotyping을 수행하였다. 전체 18개의 마커 중 2개 마커에 대한 결과를 나타냈으며, SL2.40ch07_57277111 마커의 경우 부모본 2 샘플은 각각 G와 C를 나타내며 F1 샘플은 G,C 그리고 두 가지 allele를 모두 가지는 Hetero 타입인 S를 나타낸다. 해당 마커를 이용하여 검증한 결과 G,C,S 타입이 구분되는 것을 확인할 수 있으며 판독불가인 1개의 F1 샘플을 제외하고 분석결과와 실제 실험 결과가 일치한다. SL2.40ch09_154993 마커의 경우 부모본에서 A와 G를 나타내고 F1은 A,G 그리고 Hetero 타입인 R을 나타낸다. 실험 결과 세 가지 타입이 구분되는 것을 확인할 수 있다.
Luna probe를 이용한 SNP 검증 결과
No. Chr Position Allele PIC Comparison of result(Sequencing/Lunaprobe) 판독불가샘플 Equal rate(%)
1 2 3 4 5 6 7
1 1 4686399 T/G 0.02 G/G T/T T/T T/T T/T T/T T/T - 100
2 1 80404624 T/C 0.02 T/T C/C T/T T/T T/T T/T T/T - 100
3 2 31384147 C/G 0.02 C/C G/G C/C C/C C/C C/C C/C - 100
4 2 41129419 G/C 0.37 C/C G/G C/C S/S S/S S/S S/S - 100
5 3 1578846 A/C 0.38 A/A C/C M/M A/A A/A C/C A/A - 100
6 3 60970656 C/T 0.37 T/T C/C Y/C Y/C T/T Y/C C/C - 57
7 4 59090349 A/G 0.02 A/A G/G G/G G/G G/G G/G GG - 100
8 4 62077725 T/C 0.29 C/C T/T Y/Y T/T C/C C/C Y/Y - 100
9 5 60310090 T/C 0.02 T/T C/C C/C C/C C/C C/C C - 100
10 6 40031690 G/T 0.02 T/T G/G T/T T/T T/T T/T T/T - 100
11 6 44630172 G/A 0.02 G/G A/A A/A A/A A/A A/A A/A - 100
12 7 57277111 G/C 0.37 C/C G/G G/G S/S G/G - C/C 1 100
13 8 53144148 G/A 0.02 A/A G/G G/G G/G G/G G/G G/G - 100
14 9 154993 A/G 0.37 A/A G/G G/G G/G R/R R/R A/A - 100
15 9 55798375 A/G 0.37 A/A G/G G/G A/A G/G R/A A/A - 86
16 9 67448422 T/C 0.29 C/C T/T Y/Y C/C Y/Y Y/Y C/C - 100
17 10 7318167 A/G 0.02 G/G A/A G/G G/G G/G G/G G/G - 100
18 12 3136269 C/T 0.02 C/C T/T C/C C/C C/C C/C C/C - 100
<110> SEEDERS <120> Single nucleotide polymorphism marker set for marker-assisted breeding in tomato and uses thereof <130> PN17174 <160> 197 <170> KopatentIn 2.0 <210> 1 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 1 tagtagtgcc ccaaaacaaa taagagagac gagtagaata caactatcta atcaacatca 60 cattaggaga gacgagaaga atgcacctat ctaatcaaca tcacaataag agatgagacg 120 aatgcaatta tctaatcaat atcataattc tgacctttat tggtcggtat gccataatag 180 cccccatgtg ccccaagtca aatgagagta atgagaaaaa tgcaactatc taatcaggat 240 cacagtttcg acctttgttc gttaattaac tctattagtc ttccaagcca aatataagag 300 g 301 <210> 2 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 2 aaatttgatg tctcattgaa tcaagatcag atggaagtcc agcaagtgta acaactagaa 60 acgtcttctg acactgctct aattgctttt caacatttgg agtagggggc attaactcat 120 taagttccag ctttttaaga ttagtcaacc aagaaatcac attataaaag caggatatat 180 catttgtgta cagcccacga accttttccc aaactaaaaa gtaagtctgg aatggtcgaa 240 acaatggcat caactttgat caatagattt ccataacaga ctacacaatt gagcatcaat 300 c 301 <210> 3 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 3 ccaaaaataa gtaataagtc ttgtaaccag cctcgaatat ttgaaataga gatatgttgt 60 aacaacataa tctagcatta cttctccaag aaatttcaag ttctaataga atcgtggaat 120 ctcatgtagc acgtaagatc tgtgagctag agttttaaca agcagcgaag ggtcgtgaaa 180 cttttagtgt agcagtgatt tctatgtacc gtacattgac tagcttctta gcattcacga 240 tgaagtgtat taagataggt gcatcaataa aatcaatatc catctagatc catttcataa 300 g 301 <210> 4 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 4 cctttattgt atttttttta ttaaataaat ttttttcaaa caataataaa tgacttgaaa 60 gtaataaatt tgtgtgtata tatatagtag atatggtatt cctttgactt cttcgtatgt 120 atttactttc cgttaatcag tgaaaatctc aactttccct ctgacataga aattaaatta 180 atttcatatt tttgtaaatg caggctgatg agtgcaccat tattgatgaa gcagtgaacc 240 acatcctaaa gctgaagaat acttttgaga agcttaaaca gaaaaagcta gatgatcttc 300 a 301 <210> 5 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 5 tcaattcatg tgaaaaggac ccaactatga acaagaagag gagagagcag atggagactg 60 tggataacaa tttatgaaga aaaaggattg acagaaatag cccttgaaat tcaatgtttt 120 tacagggaag cacacgcgtt gacggagaca tgtgttctat tccctcttct atatcgtaat 180 atgatattta tcatcgtggt cgattctata aatttaaaaa taaagggata atgcataagt 240 accccctaaa cctatgactg aaatctcaga gacacactta tactatacta aggtcctatt 300 a 301 <210> 6 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 6 agcaaagatt ccagataaat atgagtatcc tatattgcat aaattagtgc tcaaacatat 60 gatgcatggt ccatgtggaa acaaacgtcc aacaaaaaaa tgcatgcaaa atggacagtg 120 taaatatcat tatccaaggc catacaacaa taaatcaatc caagcaaagg atggatatcc 180 aatttataaa agaagaatgg atggaagaat agagattgtt cgtggaatgc atatgacaaa 240 tagtaactta caacccttct ctgcttacaa gatacaattg tcacatatgt tgaggtttgc 300 t 301 <210> 7 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 7 tgttttggtg gcaggtacta gtacatcgag gccttagaga gccgctggac tccagacaag 60 ccgttacgaa gcgccatcat cccaggtcag gcgacccgaa aggaccagac ataaaccacc 120 gctaagaaaa gggtaaatat gacaagcaat taagctggca agtcagcgat acaggattga 180 tttttccagg gtgtgtgaac ttccttatat attcagccac atgtgtgggt gtgaaaggat 240 tttatggtct cggtcattct tctatgaaat atcgattcta tcaatcattc catttcagaa 300 t 301 <210> 8 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 8 caggaagaaa cagttcaagc gctccaaatt ttgtgtcgtc tgtggtcaat aacactacgt 60 cacctgatca caataacttc cagtggagcc cattttccca aaccgctggg catgcatctg 120 ctgcacaagc agctaatatg gtacagcaag tgtatcagaa agtaaaacga gagcgtcaag 180 aagcccagac agcaacgaaa agagaggaag cattgaaaag gaaaaatcat tcgtccaaac 240 gaccaagtgc agctttatca gctggacagt ttaatgcttt caagaggaaa aaaggcataa 300 a 301 <210> 9 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 9 tagcatttta aaagaatttg taatcgtagg tatctcagta gcttttaaga aaccatacaa 60 tactcctaag tgggtttggt ttcaaggtgg gtcttaaaga tgtaccagaa attggagtta 120 gttccagaaa ggtttcacca ttttcattta aagaaagtta actggtatta taaacagaaa 180 gaaatacacc aatagtgtac ttctgtagta attacataaa aaagtgaaga gagagcccat 240 ttggaaaaaa aaaagagatc catcaccttt ctgcctcaga agctatcaaa aattagggta 300 a 301 <210> 10 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 10 ttttggcacc tcaatcctca cggtaataat tatgccaatt ctacctaaaa atctctttag 60 gtgatcaagg gggctccctt tttcagtaga tgagattagg atgaaaggta ttatctcaac 120 gcctcttttc tccggcttgt tctttcaaat acaattttgg cctacccaaa cttttctggt 180 taaccccaac attccccact tgtccaagac tcttcttcag acttagattc tctagcttct 240 attctcgggg tacgtagatc actaggtagc ctagctcgac ccaagcaatg cccaaaagtc 300 c 301 <210> 11 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 11 atagctgatg caagttgttt ttcttgtagt gttgtttgta ctactacttt ttgatctagc 60 tgttagtttg ttcaatttct tcagatccga atagtgcttg ttttcattgg cgtcaagtga 120 tgttggactt gtcaaagcta ctgttcttcc ggtgttgtgc aaggttatca aagcggggcc 180 agtctttgaa gctgaagaat ttaaccattt tatttttttt cttgtgcatc actaatgctg 240 agttgcctga tgagccattt gagtaacaac ctgttactcc agtcaataaa catgttgaag 300 c 301 <210> 12 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 12 aatgaatcag ccccaaaaga aaatcttttt aagaactggc cgttgatgtc atctatcata 60 tcatactgca ttttcgctct tcatgatatg gcgtactcag aggtatacct atatatatca 120 ctaataagtc tcattctaag agtagctgat tagactcagg gacgttcctt tgaggaaaca 180 cacaaacgtt ctttcatttc tcacattcat tcgaatccaa tgtgtcgtca aaccttcttt 240 taatttctgg agtgacttga atagggcatc ttgattcttt tgcaggaaga actgtaacac 300 a 301 <210> 13 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 13 gcttgatata gagtgctgga agttttataa ggaccagttg tagggatgga aggaaaagta 60 gaagtaggag gctgattatt agggttagaa gtctcattaa aagattcctc acctacccca 120 ctttcagtca cgtgagggga aaactccaac ccaagtgggg cgtgattagc ttcactaacc 180 ccatgaaaaa tatacacata cattgtaccc cccataattt atcatattga aaaggtcaaa 240 ataaccttat ctttatcaac aaggaccaat aacccatccc acttgatgaa aaaatcacag 300 t 301 <210> 14 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 14 cataattata tttcattttt tatattgttt agatttatat catttcttac tatgttttca 60 ttttgtttat agcacagcga tgtgattttt tactaccttt gtaaaaaatc aaaactttgc 120 agcatggatc aatacagata cacaacaacc aactgtttgt tcatgtcaca tgtaaaaaac 180 tgttatgata gatattacat ggacgatgat gatagtctta ctacacaaga acatattgat 240 cgcgcttcaa ttgtatctgt acatgagagg tcaattatca acatcatcaa agggtttgga 300 a 301 <210> 15 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 15 agcaataaga atcattcgtt ctgcaacagt taacgattga atcgaaagac tccttgatat 60 aaaagacgga tgatggcgta gtatatgaat tggtggatct gacgtaggcg gatttggtaa 120 ctgagtccac tttgatgaaa ttggatctaa acagaaaaac tccaaattac tagtagtgat 180 agtgctatta gtactagtac tgttgttttt gttgtatgct gttgaagtag cagtactgtt 240 actacttcga gagcatacag cgtaaagaca gtaaaacaga ggaaaagatg aggtgtaaat 300 g 301 <210> 16 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 16 gttttaagta tacgatttgt ttctgcaatg tacaggcaac atagttctat gtattctgaa 60 gcctccaaac tagaaatggt caagacaacc acagtcgcta acacatgtat atgactggga 120 agtaagaatt tcatctacca atgaaccaaa taagtaaaac ataataattc atgattgata 180 ctcgactgtc agacctagta ttcatcagta acaccaacta tattggttta agatcaataa 240 cacaaatatg caggaagtag atgactagat ttcacttcca tagctagcct cttcacactt 300 c 301 <210> 17 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 17 aaaagcaaca accgatccaa tgaaatctcg tcattagagt gcatgcagac gttatgtctg 60 ctcaatcaaa tcaaaacgag taagtaataa ttcaaagaga aacattacta gtaatcatac 120 ctgaaatctg cacatgtagt aatcatctcc gaggaaagct ctcatagtag gaataggttt 180 cattttagga ttccttggcc taaaaggcac actaagacaa acatatgcct tgactaaatc 240 aggcctaaac atacacaagt accaaccaat catagcaccc caatcatgcg ccactaaaaa 300 c 301 <210> 18 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 18 tgcattgttt gagcctcaac atagctactt tcgaagattg ataacaaaag tcattgtttt 60 tatttcaatc atagatgaca tttatgatgt ttatggcacg cttgatgagt tggaactctt 120 cacacttgct attcaaaggt aattataatt ttctatcgca aaataattac aaaatttgat 180 tttctagtaa aaattcagat gggatacaaa agcaatggac caaattccat actctatgaa 240 agtatgttat cttgcactca ttaatattat caatgaagtt gcgtatgatg ttctcaaaaa 300 t 301 <210> 19 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 19 cataaacctc atcaactcca ctggctgctg agaattggct attcccggag acgttgctta 60 ccgtggagga ggatgtagtt gttgtcgtct gtgatgatat gcctacaatt aaattaattt 120 tattattcgg aagtaaatca aattctgatt catattacga atggtacctg agctgaaatg 180 ggatgggctc tgactattag aactgaaaca aactcccata tctctatctg atgattatta 240 agcaagttat tcttcggctg aaagagggat gggatgagat tgagatctct tctcttctat 300 t 301 <210> 20 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 20 tatcttacca tatcatgttg aggaatcccc tctgttatgc agacaactaa gtccaactca 60 gcttccatgg cctccataat agctgcagca gcaaatggtg gaggaacata aaccactgaa 120 gcattagcct ttgtctctac ttttgcctct acaacagtat taaaaacagg tagccccaaa 180 tgttctgttc cacccttctt gggggtcact ccaccaacct attaatgcac acaaagcata 240 catgaggaat tcaggaaaaa aaacaattta cttcttaaaa tatagtaaca aataaaatgc 300 a 301 <210> 21 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 21 aatttctacg tacaaaccga actcccaccg actccatact tgagcagatg gcttagatcc 60 ttgttttcta tcttcgtagt tcagaaaaaa ttgcaaactg cactgcgaat aacataagac 120 ctctattcta gcaagtaata tgagccttaa gtgattacat ttatggaagg tagcctgtag 180 atatttttta atgcctgtca gtgtataata agttaaaaca ctatggtaaa agaggaataa 240 tgtgttggtt gaactgcgca atagcttcgg aatcaatagc tcagtgagat aggccacccg 300 c 301 <210> 22 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 22 tcttttcttt ggtggaagtg ggaagaggct ttggggtttc cgaactttgt gcctctctct 60 ccaaattact tctaacgatg aagttgaaat tgtttccaaa tcactttact aacaaggaaa 120 gaaccttcta ccctgtaggc ataaagacgg tgaaataaaa aggcagctgc ttcaagttct 180 taaactttta actctgagaa agaacatgaa caatttatta tgtttctatc acaataagaa 240 caatagtatc taacgatgtt attgatatgc actaagtact gaattgtaga tcaaactaaa 300 a 301 <210> 23 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 23 agcctagcaa tgtttcgcta gacactgata ttaacccgaa gatatctgat tttgtcatgg 60 caagaacttt cggaggaaat gagtctggaa tcatgacaaa aagagttgtt gggacatagt 120 aataatcttt aaaccaatga actatttgca atatgtgaaa aatctcctta ttttgtttat 180 ccgacttatc ctacagtggc tacatgtctc cggagtatgc agaagaaaga atattttcag 240 tgaaatcaga tgtatttagc tttggagttc tagtattgga gattttaagc aggaagacaa 300 a 301 <210> 24 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 24 atgtcatatt gttcaagtaa gaactaaata tttaattatc aaacctaaaa gcaccataga 60 attcagccag atcaatttaa caagtaaagt tgtaaactga agtgctgtag aagttgaggg 120 ctgggtcctt gttcaaatag aacaagcgaa gcaccatcac agaaaattat ttatattcag 180 taaattagta attccattaa aaatgtattc tcctcataga gattcaaaac ttgcatctca 240 aggagagaga attggtacaa ctactttaaa gagttctaca aaagcatcaa cttctcaaca 300 t 301 <210> 25 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 25 cccttttacc aacaaataaa aacgaaagaa caaagactga atgctcttgg agatacttaa 60 atgtccgccc cttttattga gaaacatttt ttcagctgga ctgaaacaaa aaaactttca 120 acaatcaaca aaatatagaa ccgagaatca aagcactcaa cgttgtaaag aacaacctaa 180 cagcattata agtcggactt ctccaataca caagattttg cttccagaga cagatcctaa 240 actgagaaaa tgcaccttga ctgtatatgc tggtgaactt tagaggttct gagttctcag 300 g 301 <210> 26 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 26 aaaaacagag ttggttacat atttgttgaa ttattcaaaa acagaaattg agctgcgtaa 60 gttctggctt ctccttttgt tggttaacat ggatgatact catgatagat ctcactctca 120 ctctctctct ctcacacaca caaaaaaaaa aaaaattaac acttgctagc catcttgttg 180 aaaaggcatt acctggactc tgaataaggc aaatagtagc tgcattgtta gtacctctat 240 ttttattttg cttgtttttt atttgataca gcaggggtgt gaggcagtga aaacaaggat 300 t 301 <210> 27 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 27 caactatttg ttgttgcgag gactaataaa cctctattat ggccagtgta attagcagtc 60 aaagggaaaa cggttagcat tagtaatgat taaagtattc atttgttccg aagaggggca 120 cacttaagaa cagaggagtg gctgagtagg agctctcaac tccttcgtgt tcccgatctg 180 cagcatttct gttgcagctc ctttaagact actttatttc taaacacgtg ttcctagcaa 240 ttgctacaac cattgcagta gctatttttg agtagtgttg agttgtgcgg agatgcatac 300 g 301 <210> 28 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 28 gcactagtac caggttatat actgtgctcc ttctaaattt cttattgtta ttcagggcag 60 agtagaagat tccaacccaa gcccaagtta caagtataca gagagagata tgatacaatc 120 attcctgatt tagatgtagg agagtctgtg ccatgtctgc cagattcaca atccgttcat 180 tctgaaatgg attttgtgaa ttctgagaag ggacatggat tacctgctgc tgatgtcctt 240 gatttctcag tcagaggatt tgatattatt acagaatctg cacatgattt cccagttcat 300 g 301 <210> 29 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 29 ataaaataat cctaacgccg tttgttaacc ccaaataaac aaaaaaaaaa ctactagcgt 60 ttcttcaacg gcagaaggac ctataaattg gagccaatta caatgcaata agctgcaact 120 tttatcaatt ctctttatct ccgaaagctt cagagttagt ttcttcgtct atcaatggcg 180 ttcaaaaaac ttgtagctca acgtcttttc gattcataca aaattagtcg tccttcccta 240 tcaacatgcc gtatttgttc ctcaaccatg accaaatcga tggcttctcc taatcctgaa 300 a 301 <210> 30 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 30 ggagtatgat gagcagtttt ggacccaaaa acaagacgaa attgagggtc tttgtgcact 60 gaaacacatg aacccatttc ttcttcaaca atcacaaatt aatccataga atttagagag 120 aagaagaaag gtgctgcctg ctactattac ccagagtgac tgaaaaaact cttcaattgg 180 gacttgaaag cgaagttaga atggctttgg tgtggtccca cacaaaaagt ttatatcata 240 cgcaccatgc gtttcccctt ccgttccgtt tcttcctaaa gattttttca tattcgaaaa 300 t 301 <210> 31 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 31 ctcttgtgct actagaaaac agtgaatgaa tcatctcttt atcaccatta aatgaatgtt 60 atatggtgtg tgagaagatt attttttgat gtgcaattat ctgggaatgg aatttatatt 120 atcgtactaa tattcttttg atacatgtgc agtgctattt tcattagtat tctataatta 180 aactggagtt ttcttttcta ttcaagcacc gtgccttttt tcctcagtgg acttgatcag 240 tataacttat agtattatgt gtatttgttc ccagggaata gtatgggttc tagaatctga 300 a 301 <210> 32 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 32 acaaccaaaa ctaaaatgca gagaaggtgg gagactagat aagtccatac ctatgaagag 60 aattacatat cgaagaatgc gaactgttgt tagttcttgc agaacccata ttgcagcaat 120 aaaaatgata aatcctggca agtccactct atgggatcag tgactaatta cagaagcaaa 180 tgcatatcaa gtattgacac tgatcaagat cctgaatata caaacctatg caaagtcctc 240 gcagtgtcca ctgttcaaga aagtaaacca tgtaagattc ttgaagtcct ttttaagaaa 300 a 301 <210> 33 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 33 agcacaaagt caaaggctct tttctctcct tactgtgaca ctcactctaa agaccttgtc 60 caacatctcc taggtatacc accctcatct ctagtcagga attaccttgg cttccctatc 120 ctcaaccata agcctaaaat gaaggacttc tagtacatca tcaataatat gagaaaaaaa 180 ctcgctagtt ggaaaaaaaa ttcatgaccc ttgctggtcg catcaccctt gcaaaactat 240 ccttgacatt attctagcgt attctacgta atattttcga ctccctaaaa aaatttgcaa 300 c 301 <210> 34 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 34 aaaccataca tatcaaagat gactacaggc tgtcctgtgt tggtagtctg tctaggagga 60 gttttgagca caatggtggg ttcttgggta gcatgaatgt gtctcaacct tgcagccaag 120 gtctgaacca cagtgtaggg agcaggttca agtatttttt actgttggat gttttgatgg 180 cttttaagaa ggttgttttc tatcactttt tgatgggggg gtgggctgga ttttgtttat 240 caaaattgct agagactttt ggaaaatcac tatggtaatt tgtttgacct ttattccccc 300 c 301 <210> 35 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 35 ttgacatcgg agacagatct cctcaagaga tgtcttgtcg gttaattcca gagcacactt 60 cgggagatac caaccctcaa tgatgtaaga aggtgggctt gcaactcttg gaagttagcc 120 ataggagtta gcgttttcgc tattaacaac agacacttcc tgttagagct acccgcaata 180 gtggctgcag tgcatgtcat ggcaggaacc atgtatagtg gctgcagagc acgtcatggc 240 aggaacatgg ttttggaaga agatgcatct gaacttagag tggtggagcc caatcacagg 300 t 301 <210> 36 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 36 tgaccaaaac tcatcctctg cattatctac atcaaaaagt cccaattgtg atcaaatgcc 60 tctggatatc caacttgcat aggatccctg gttggttacc ctttttttag aatcaacaca 120 ttcatcggct ataaacactg catccatgat ttgcctgcac ttgataaagg ccatttgttg 180 cctgttcaca agcttatgca tcaccttctt tagaagagat tgttattcct taaactaaat 240 gattttcctg atttggatag atgacactat ttttacttgc gttacttttc ctttaatgag 300 t 301 <210> 37 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 37 aacttcactg tgaactttaa cttcaacgtc aatgtgaacg tcaatgtata ctatgaacat 60 caatgtaaac ttaaatgtca acgtgaatgt caatgtaact gtaagcatga atattaatat 120 caatgtaaac atcaacgtga gcataaactt ttatgtgaac gtctaagcga gagtgtatgt 180 gaatgaaatt gtcaacataa acatggacgt caacgtgaag gtcaatgtga ttatcaacgt 240 ctatgtaaac gttaacatcg acatcaatgt aaatgtaaat gtgaatgtga acgttaacat 300 a 301 <210> 38 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 38 tttgctctct ccgcgtcgat cctctaatct ataccactga ggtgccaaag ggctgtcagg 60 tggaaccctt gtaggcactt cattcatgtc aaacaccact ttgcctaaat aatcatctct 120 ctgcaccatc tctttgtctc ttacaaaaac atcgataact gaagactgaa tcttctcttt 180 ggaaaaagca aacacttgct tccactcagg gtttaccttc ttatcgaagt gctttgtttt 240 gcctttatag tttccgagtt tgacctcgac ataaggatca caactccctg tcacagggtt 300 t 301 <210> 39 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 39 tttaacggga gaagttctac aaaagaaagt catacaactc cctaggtgtt ttttgtgcaa 60 cttgacaggg ttaagtaata accatctctt cctgaattgt aagtttactg tccaaatttg 120 gaacttgttt ctcaacatca caagcatgaa atagacaagg ccagaacata catctgatct 180 gttgagttgt tggattaggt agggaggaag taagagtcaa aagagatggt ggaaattaat 240 accatcatat gtacggtggt cagtctggaa agaaagaaat gaaagatgct ttgaagatag 300 a 301 <210> 40 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 40 gcttatggga gtcatcagtc acaacttcct caatctattg gattgctcaa acaactactc 60 cctaaatatg gtttgaacaa aatctgtgtt ttttatagct tatattcggt taggagggca 120 aataatttat taaaaatatt ttttcatgtc actttgaaat tgaattgtaa ttatcacata 180 gattgataga aattatgtca cgccccaagc tacccccgag acgcggacac ggaacctagg 240 accacaagtg atcttaagct aaacctgctg acatgatcat gagcattcta aaaataataa 300 a 301 <210> 41 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 41 tgaactttga taaattcatt aatttttgga tcataaaaag actgtattca ttcaaaaaaa 60 atttagagat ggacatgcta aagacaacaa caatagacgt aatgcataaa aatgttctct 120 tttaactttg atttctgtta atatttatgt cccttcaatt tttaatgtgt acaaatagac 180 acttaaactt atataaaatt gaataaataa cacatacatc ctacctgata atttatatga 240 gatatattcg aactgatttg tatttgtcta gttgttcatg ccggacccat attatatcat 300 a 301 <210> 42 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 42 tgaagcagga atagaagtcg gggagaaacc aggatacggg gaaaatctag agtcccagat 60 tgacatagaa attagtggag cagatgatat gaatcacgac atgccagata tggtcgcatc 120 agagagaaat gtcgccggag gggagacgtt caacattcag gaccctgcat ttgttgaagg 180 aactggtggt ttccatgaag agcataggcc gctggaagag gagaaaaata agaacagtac 240 tggtctggtg atggataatg gccagattaa ccagccagag cgggcacata ccccgcaaca 300 t 301 <210> 43 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 43 atgaccacac gtgacacacg tcttggtttc tttgccaata aagaacctca taagctgaaa 60 ttcttggaga cagaagaaag ttttgagttg ttggtaatga gagtttttgg caggggcggg 120 tgtccaaatg aattagtggt aactgggaca gaaattgcaa gaaaatgtgg tggagtacca 180 cttgtagtag tggtgattgc aggagcatta agaggtcgtt cggacaaaaa ggattgggaa 240 agagttgaaa aaagtgtggt gcaatatctt ggtgagcata ccgaagatag ctgcctgaaa 300 t 301 <210> 44 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 44 gcctatcaaa ggtatgtcac atatcaaact cggtgtacct gctattgcat tgtatatttt 60 acactctatt gatcttgaat gctttgatat gtacagtatc ttcgcagtcc gaacttgtga 120 agtccctcct tggcacagag gtaattcact ctctctattg ttgctgtgta ctttgatctc 180 accatggatc atttagttca tgaatggcaa tgaaacacag cctacttttg caatttgatt 240 attctgttgt aatgatggtt atgatatgaa cataaacttt gtttaatctt tttcaagact 300 a 301 <210> 45 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 45 taggataaac atctctatag atttctttga ttgcttgaga aatgatcccc acaacatcat 60 ctgaattgaa actgtaatac aaagtttagc agttacaagt taaaacaaag acaattaaat 120 catatgataa taaacatacc atattttttt aaaaaaaaac ctacgtgtag ccttcaggct 180 caatgacaag tctattatga atgtccctca tgccaacttg tgtattgtca tgaccaggtg 240 tatctgactg atttgtggta ggagtgttag gctcagaact attaccttga atgcgaagac 300 t 301 <210> 46 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 46 ggcagcagta cgccaaaaat gactattgat gaggcattct cttatttgta ccaatttaat 60 tacaagtatc caaatcaaag agagaagtat agcacattcc ttgttgttat gatgggcttt 120 atgataaaaa ggtttgctac tacaattcgt ctattgtcaa tcttcactta tttctattct 180 tatttgatga tatatgtgga actaattttt aatattgctt actgaattga tattgttggt 240 gtcattgaaa aggggaaaaa tttattcaaa gggcattcta gtttactcct tggtttaaat 300 c 301 <210> 47 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 47 taatcaattt ggtaactatt tttacctttt tataatgtcc atctaaaacc ccaatttttg 60 gggtgtgatc atgtgattat gggttgattt agtttatggg aatggctagt tgttagttta 120 agtgttattt aaaagtgagt ttaatcatta attgtggctt aatttaagaa ttgtagttac 180 aaatgcagtt ctatttttgt gttcttgtct tgctctcgag agaggtttta gaactaaggt 240 ttttgattaa tggtgtgtgg gtattggggc cgaactgggt ttagctcgag agggtgaatc 300 c 301 <210> 48 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 48 gttctattgc aggcatcaag tagatgcata atagttacaa aagcttagta gccaaaggtc 60 agctctcctt tacattgtta attgagaatc agggagcaga catagttcag aaatgaatca 120 gggtaatctg gaggggataa ataaacccct gtagatttta aagattggta gggagttagc 180 ttttaatgat ttggtaggta gtcccttctt catttaacaa tccattcgta gtataaattt 240 tttgattacc aagaaattcc cgagtaccag cgatgcacgg tttggaagtt tagataatgg 300 g 301 <210> 49 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 49 tgtttcgaat ccgctggttc aaattggttg tcaatagaat agtgaagtag ctagtgacta 60 atcttttcaa atgtattctg ttgtcttttt aaaatgtatt ttgatgcaga aaagagcaca 120 acatgtacta cacttgcgct ttctaaatat ctaacttagc ttcaaaatga gtattatata 180 tttagctcta gtaaattgga taagcaaaaa gtgactagta aactgggaca atggggtgtg 240 tcagtatttc tataatggcg atgttcgggc tagcttgggc acctagacta ttgcacagtg 300 g 301 <210> 50 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 50 atggactgaa agtagtggta ttattccaat ttgtcacagt gtatgactat agaattctgc 60 tgtttgtcca atgaactgat gacgcatcaa gcaaaagcaa gtcaaatcta cgaataacaa 120 tcatctttgg ggcttaaaaa tcattttctc ctgtttgttt taggagaaaa acttgtgtga 180 agattgcatt ttaaaagttt tccctatgat gtttgtttgt caattctgaa tcctgttgtg 240 tgcattcatc gagtcttcca ttccacgagt tctagtgacc tgcaatttta cttattaacc 300 a 301 <210> 51 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 51 catgccatca tacatcaagg aagatgtacg atcagtgctt ggcctaccag agctgacgaa 60 tgataaatca gtatccgctt cagaaaggtc agaaaagatc cttgaaggga tgactcttcg 120 tctatcaaac ggggacctgc ttccaaaact cgctggcttt agtgtctata cattatatat 180 aatcaagcag aacatcttat tcttttgtag caatagcaat agccagaaac atgttcagat 240 aggtgtagca atggcaataa caggtcttta aacttaccca acttttctgc tgtcatcaga 300 t 301 <210> 52 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 52 gtgagaagat catttaattt tcaacagttc aaatcataag catagtctta ctatttcatt 60 tcctctaata tttctgttcc aatgctactt gggtattcca aataagataa gtcaaacaat 120 gaaaaccgtg atctatttct tttatatggg cgcacggata taagaacatt ttctggatta 180 ttttccagtg ttcaaatgtg tacaaagtga cttgagatag tcaaggtgta atttagactg 240 aaagacaata ttttgatgat actacttatt agttgaagca aagtgcaaca aagtaagagt 300 a 301 <210> 53 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 53 aactaaaagt atattttgtt tcaatttctc agcatctaac tcggcaagat gcattccagc 60 cttgggagga tgcagaatgt ctccagacta tgcctggatc ttcctagaag ctgttgcttt 120 ttcccattct ccaatgtcaa tgtgtgacat taaaacaagc aatggaagaa accaacaaca 180 attattatgg cttaacccta aataggttag ggtcggctat atgaatactc actgaccaaa 240 ttccttatat aagctcaact cgtgtcgtat cattgtaccg ttagtagaag caaccaaaag 300 a 301 <210> 54 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 54 gataggtgga agtgaagcat gtggtccgaa gataatcctt agaagtataa agggcatcca 60 aagaagtgaa gaagtgttga tttcatacac tgacttgtta cagcctaagg tacagttgtt 120 ctcaatcttt tgaaggacat gacacaattt tatattatca aggacatcac tatagatagg 180 aaagagtgga tgtcgtgtat tagggtagaa ggctagtaga agagagtgtc gcctttcctt 240 gcataggttt agtgcctgtc ataggactag ttgtaccctt gtagttcttg tggtatgatg 300 t 301 <210> 55 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 55 gttcaagaca gaagaaattt atcaagaatg gctaggaatc aagtgtgttg ttgctgtttg 60 atattattgg tcagcaacta ctgactgaga gagtattaga attccaactt ttagctcttg 120 agtattgaca atttttagct gggaagattt tggtttcaac gaatggcagt aagagccacg 180 cgcaaaatgg aaaaaaatca acccacatac ccgctccgcc ccgcaaaaac tcttatccgc 240 actgccacgc aaatactata acccgccttg ctccacaacc gccctgccca tgccatccct 300 g 301 <210> 56 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 56 taccattaga tccaatgtat gggaagtgaa cgatgagcag ttccttcgac tcagaatctt 60 gaaactagaa aatccttctt tttcaagatg gtatgcctca gatgatgcct ttccatgcct 120 tgaacgcttg gaactgaaaa gatgccagca cctcaagaac atcccttatt gttttgagca 180 ttcttcgtcc ctgaaatcgc ttaagattat atcctgcaat gacatgcttg ccaattcagt 240 catgcaaacc aaagaatcat caaagtattt gcacggagct tctggtttcg aggtcttcat 300 c 301 <210> 57 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 57 actttagaga gataaaaact gaaactatta tacacttcaa gaaatgaaca tataagaata 60 aataacaata gaactaataa ataatatgat aactgaagaa aattaaacaa tacttaatat 120 aagatccaag tatgttgagt ttttattcta agccacataa atatagaact ataaagtttt 180 atatgaataa aggaaattaa taaaagcaaa caccgtgatg ctagtgatga aatattcagt 240 ctaatccttc cttctgagca gcaacaacag cctttgaagc gttactcttg ttgttaggct 300 g 301 <210> 58 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 58 gttgaacttt ggaaaagtat acgcctagac ccagatgggc ttaaacggat cgatatggac 60 agtgagaaca catagaccca catgggctta aaagaatcta aagatttcca cttatcaaca 120 aaaatatcta aagatttctt ttttatgcca atcatactct aatgaactca gcataaacct 180 gtcagcctgc tggcccgttc ttcaactctt gaatcacgag tactggttcc agaaagaaaa 240 acaatgtctg ctttgaaagg aatcctagca gaaatctgga ttaaaaaaac atacttaaca 300 a 301 <210> 59 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 59 tttcgatcga tctccagtca gattcgaaag aatatgagta ctaaggtttg ttcgcccatg 60 gatggggaat ctttaagtcc aatctcgagt caaatagacg gcgaagtcac cggctgcgaa 120 aattccggtg accctacatt tgctatcgcc tgaattaatg gtgtctcagt agaaaagaag 180 gactctcacg gtcaaagcac tcacgtaaca acacaacctc atcaaacaga tagagaaatc 240 aataataaca acatcacttc ttctcaaaca gatcacaaat ttgatgaaca acaagagaat 300 c 301 <210> 60 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 60 agctgcaccg cgggaattat ccttctccaa tgttggcagc tgtatggaag agtagccaaa 60 catcaatggc aaattagtag gtatgacagg ctgggctcca ggtctatctc cactagtggt 120 agctattact tgcatcatgc caggaactgc ataaccagtc gtacttgctg tattaccagg 180 gttagaatct ctcagaggca tagagtatgg taggttcatg atgtttattg attggccttg 240 gaatggggta ctgtatggcg tattaccgaa cttaacatcc ttttcagaag aaatggtaaa 300 c 301 <210> 61 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 61 ttcagatcta tactcagatt caaatatcca attcttaata aacatcttaa tattcagatg 60 tgtattgata atcaaacatc ttagtcttaa taaaaacaaa tgaggcttaa cacttccaag 120 aaagctatta tgtaaagaac gaggtactca accatagaaa tgtcatcata aggtcatgtt 180 gtaccattat tgaaatgtga accttatttt gcatcgtgaa ttagttggat ggaacaaaca 240 ttgataagct attcctctta gaagataaaa ccttaagatt aaggtcctcg agacctggac 300 t 301 <210> 62 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 62 gtggtggtgt tgttggtggt gttgttgttg gtggtggtgg tgttggtggt ggtgttgttg 60 gtggtgttgt tgttggtggt tgtggtgttg ctattgttat tactattatt attgtagttg 120 ttgttgttgt tgctattgtt gttgttgttg atgatgttga tgttgatgtt gttggtggtg 180 gtggtgttgt tgttatagtt gttgttgttg ttacttttgt tggtggtggt ggtgttggta 240 ttgttgttgt tgttgttaat ggtgttgttg ttgtgttttt attggtgggg gtgttgttgt 300 t 301 <210> 63 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 63 tacttttact tacctcattc aaaagttcat caagggctga taatgtcgag gattgaataa 60 aattaatttt atgctcaatg ttagcctttt taataattgg caatcctatc tcgtatgcat 120 ctcgatcaag gtctatagct ataatctgca aaatatttgc acacagagat caatgatgtt 180 ctacacagag aatatattat acattaaacc aattttagtt gtataaaatt gtgatgcact 240 agccttgcca tcatcaggaa ttgtaagggc aataaggact aaggaatatc cagtgaacac 300 t 301 <210> 64 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 64 ataatatatc aatgtatcaa aaaggtttag ccacataaca agcattcaat taacaataaa 60 gatctaaaag tattttatac ctccataatt attcccctca tgttctcaga attatttgga 120 acagctttac ccactcgcac cctgaagtca tcaagttgat actgaaatcc ctatataggt 180 aaaatattta tatgagtatt atgaaattgt gctttgtaag ttcttttcta gaatttttaa 240 acaaaaggaa aagcagtgca aatagtgtta agcataaaac aaatgtcttt ggcaaatcat 300 g 301 <210> 65 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 65 attgtttata tggatccttc ttgtgttaaa gtacatagaa aaatgacatt gccaaaaaat 60 tgacaaggta aaatttccat ttattcatcc aaggaaacgt cctttttgaa gctggaattg 120 attttccttc atacctaaca tgatgcatga aaggatcctt gaataaccat agggtaacct 180 gaaaatcctt agcaaagact tctacaagac gttctattgt tccatagaaa tatatttgtt 240 caagaagggc tccaaaagat gttgatcgta aatgagaaga ttggttctgt agaaaaacga 300 a 301 <210> 66 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 66 ctccttcacg cgctaatggg gacaacccat aaattgctcg acaattccag cagcaatgaa 60 gagcctcgaa aacgtgtgaa gcagcaaact ttgaaaatcc cacgtgacta gtatccttcc 120 aggcgggtac gatttggaat agccttttcc atgattttag cccgttgttc tctgcgccag 180 tcctacgaga ttcatcctta tttgtccttg cagtcataag ataaattcat ccctagtttt 240 atatggagca tccacctcac ctccctcctg ttaggattta acgaattttg ggcatcaaat 300 c 301 <210> 67 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 67 tagctcgact tatgtacgta tcgaaaaact agtgattcac catttgatag gaactggata 60 ggagaatctc attgttctat cttttcttgt gtaaaatgta ttctgtaaat tagtgttcat 120 ctcatcttaa attatatatg ctgaaccact atttgtaaaa tcttcgactc tgcatttccc 180 ttggcatttg agggggagtt ttgcaagaca caactgaaat gcatttgata agaccccctt 240 ggtgggaggt cgtttctgat agaccctcga cactgcttga gctaatgttt acaaagtatt 300 t 301 <210> 68 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 68 aagaaggtca taagttcaag ctacctctgg tagaaatgta agagtttgta caatagatca 60 aatgtgatct tatatatcct ctcacaaacc cgtgcatagt gggagtttat tgcaccgaaa 120 aactatttat tttttactct ctccgtttca ttttatgtga ggtagtttga ctcggcacta 180 agtttaagaa ggaaaataag atttttaaaa cttgtggtcc aaaatgaatg atagaaattt 240 ttgtgatggt aaattatttt attaaggcta aaatagatat ttttacagtg aaattcttgc 300 t 301 <210> 69 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 69 atcacaaaaa taataggaaa attggttttt cataatatct ttaaatatta tgataagatt 60 tctaagtagt aagatgtctt ctaaaggcat tttacattaa gctttgtttt ttcagaatag 120 gtgaaatgct tatagtttaa actagataat tgtaggaaag aactatttcc aattttaaat 180 tttatgaaag atttatgcag ataggtttcc aattaacata ttgaattact taggtgttga 240 atgaaaagaa actaagtcta aaaactaagc agaattgcca ctttttgcac cccttggttg 300 t 301 <210> 70 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 70 gttgatgttt atctgtacgt ttatgttcat agtaacatta atggtcacgt taacattgac 60 gttcatgtat atattgacgt tcacgtttat gtttattttt atgtttatgt tcatttttat 120 gttgacgttg acattgatat gtacgttcac attgacattc acgatgatgt taacatttac 180 attgacgttc acgttgatgt ttacattatc gttcaagatg acgattaagt tgatgttgaa 240 gtcgaaatcg ccattgagtt cacataaaca tttcaattga cgttcacatt gatgttcaag 300 t 301 <210> 71 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 71 ctaatgagag attctttttg ttttctttag gatatctatg gtatgtaaca gtgacatact 60 tcttttagtt aagtctttcc atattaagag aataagttgt tgcttatgtt ttttttactt 120 gtttatgttg cgcgtgaaat caaattgggt gaaaacttgg actccttgtt ggcatttttg 180 agtttattaa agactcatca tctgttgagt catacttcac caataacagc aacaactgtg 240 aggctggaaa ttgaaaataa caagtgtcac agagctgaaa gctacctata taccctcttt 300 a 301 <210> 72 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 72 ttatgatcta ttaattgtgt gtagatgcca gtggcaaagc tcaacacagg tggtactcca 60 gatgcaaaga aaaaagtaga tcaggtgaac aattcgcttg attctttcat taggcaagct 120 atgggaaaga aacccctacc aagaacgggg ataggtcctg ttcagtggac gcagtggctt 180 cattctattg atcagccagg taacaaattt agaacttggt gtttatagaa tacaacatgt 240 ctttttttaa tgatttaacc atccagtatc taaagtccat tgccatagta tgctagtgtt 300 t 301 <210> 73 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 73 cttcgtctaa gcctttgaac gtgaaaagaa gtggagcact tgatgacaat gacccctggg 60 ctgctattgc agctcctgtg ccaacttcct cagcaagacc ttcaattggg cgaagccgag 120 gaaccaaacc cgcagctcct aaattgggtg ggcaaagagt aaaccgaaca tcatctggaa 180 tgtaaattac aaaagcaaac taaggctccg cagcagattt gagttgatcc tgtaaatttt 240 ttccccgagg cggataaagt tccaactgaa atatgtaaat ggaagttcta tagatgtgtt 300 g 301 <210> 74 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 74 ttaaggctga taaatggagt tattcttttt taacacttgt ccttttattt gttaaaaata 60 aaacttaact tatacagaca aatatttcta atttggatta taacatagta tatttgaaag 120 aaaaaaatta atattgctta tgatgcaaat aaaggttgct ttcaatgtcc cgtgcgatat 180 gcagaatatc agctaatttg gtgcctccaa tcaatacatt cattcactga caaatactca 240 ctcaccccac attcttgctc tgcaccttac tggacttaat ccttaccctt tacctgaccc 300 c 301 <210> 75 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 75 tgaggttcca cactgccact acatccattc gtacacactt tgtcatctaa ttttttctca 60 tgatctggaa gtatgaaatc ttgaatagta tccacttttg gagattcata aacatagcta 120 gagaaccagt tccggatgtc tggaggctct cataaaaatc atatagggta acaaagatta 180 gcattaacaa aaactatcat tgcctgctta aaatggaaac ttttgtatta ggaggtgaaa 240 tgatcacact aacctgaagc aagtgacata tcagctgaag attccacatc ctaaagacac 300 g 301 <210> 76 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 76 aagcaaagct gtaatctaag caaatgctaa aggcacaaac ttgccatttt aagcaaatca 60 ccaagaaatg taacacaaga tcaaagataa agatttcaag caacactatt aacagcatat 120 atgatgcact aagactctat tcagtgccag aattaccccc attaataaac aagttatttc 180 agaatgcttt ggggctttat ggaaaagctt caaaactgtt atgtttggca agacacattg 240 tgggtaagtt atgtgcatat catgtgcata aatcttttga tgaaatgagc tacaatttaa 300 a 301 <210> 77 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 77 tctgatgatt aactttttta caagtcatga atgtggagtc atttctaact gctcgaccgt 60 cactatttgt aagcaggcaa tatttagagg ggccatgaaa ggcaagctca tagtgacttg 120 tccccttcct ccagagagaa taccaaagtt ccaattgctg tacaaagatg tttagcaggt 180 atgattgttt gtaaatttag gttgactagt tcgtaacttg acatatttct cagcttgagt 240 ctaaattata aaaagtttaa cgtgttgtcg gtatgaccaa gaaatatttt tcacgagata 300 a 301 <210> 78 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 78 ttatacccac tttaactaat tccatggaat acctaccatc tcccaccatg ataaacatgg 60 tgcccgagcc aactcgtgca cacctcaact aactctacgt catacgtgcc acctcctttc 120 agcaacatgt accaggtgga caaaacttaa gcgcacctca actaattaca cggaatacct 180 accacctacc accatgataa ccatggtgcc cttctacgtg atactagtcg actcccacca 240 acaatatgta ccatgtgact ctatccacca acgctatcac caacgctaga aatagactaa 300 a 301 <210> 79 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 79 tctttaaacc aaactcaaca cggacttctt taaagctttt gtcgctggaa taaaaccttg 60 tagcattagg acttctcttt gtactttgca ttgaaaggaa acttcttacg tgatttttgt 120 tatttagctt acaaaaactg ttttcctact ttaccaaacc tagttgaact caacttggat 180 taggtaatat tttctccaaa cacgttttcc gatttagact tggattcaac tacaacgtgt 240 taatacttcc ctgtgcgtgt tgcatgtaca tgttagtact acatgttcta ccgtaacttt 300 g 301 <210> 80 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 80 aaaatatctt caacatatga aaattagtac aaatcttaac ttggaagagc atttacaaga 60 acaagactca ttgaaaaact tcccaaattg cacgctgatg tgatacgcaa tgaatttaga 120 agtgaagctc atgagaagat ctttcacaca cgtacctttg tgttggtata taaatagcat 180 acgagggtac atgacttgtt aaacagaaga tgagccatgt tttatactta acatgttcca 240 gattctgtga attcctccct tttttggtgc aaagactatt aaaacgggaa tatgttttat 300 g 301 <210> 81 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 81 catattttgt acctccgaag cgacagtttg ttccactata tccaattata ctttaagcac 60 aattgttaca atatatactt cctcgaccac tttatgttgc acccttttgg taatagacat 120 aaatatccac acctttttta tttatggaca ataagttcct tttattgttg tccacctagg 180 tttttttact tggttattga agaacattgt tatccttgag ctagttgctt ttctttttcg 240 ttttaaattc ttttgtttgt tgtcattctt gttctttggt ttgattcaat aacatcaaac 300 a 301 <210> 82 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 82 atcccaactc ctttctaatc tctcaggccc aatcccctat cccatctcaa ttctgcaggc 60 ctaacttact aatacccaac ccacttttcc ctatacagat tctctttttg ccccgacccg 120 tttctctgtc gttcaaaaaa gatgagagca ctagttgtac aacagagaag cttcatcaca 180 gagcgtgtag catcacgtga ttccaagcag ttgcgacgtc cattgaacca gacaacgcaa 240 aaatgcgtga gcaacgacac tctttcctcc aatgtctcct tgagaagaaa atcctgtagt 300 t 301 <210> 83 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 83 atcaacaaca acaacaacaa caataacaac ggaaacaaca acaacaacaa taacaacaac 60 aacaacaaca atagcaaaaa cagctgcaac aacagtaaca tcaataacaa caaaagcaac 120 aacaatagaa gcaacaacaa tagcaattac aacaataata acaataacaa caacaacaat 180 agcaacaata gcaaaaacag ctgcaaaaat agcaacaaca actttagcga ctacagcaac 240 aacaataaca acaataacag taacaacaac aaaaacaata acaataacaa caacaaaaaa 300 a 301 <210> 84 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 84 ctctatgtac gaatttttgg aggtcaccag catgtcctta atgctgagga atacaacatt 60 accgtttttc aaaaaaactg gcttgaattg gatccttaaa atttctctta atggaggttt 120 agatcctgaa tcagtagcaa agagccaaat cggtgaatgg agtaatcttg gctggaaatt 180 ttattttttt ttgaagaagg taacttgtat tatgaacaaa aggaatactc agaaaagtat 240 tccagtatac ttgcaacaaa attacagagg gagaggtgtc cctactaagc ttcataaatt 300 c 301 <210> 85 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 85 ctccagttag agttcccaga aagaagcatt ggcttttttt ctaagcccat tcaccagcaa 60 taatactgca tactgcaaat tgataactct acagagcatg gcatgcacat aacaaaatcc 120 tatgtataat attgtttata ctgctcagtt cgtagaagga agacatttat gatttaattt 180 tacccgattt caagataaat ttaaaaagta ttcttttgga tctcccgggg tacgattcaa 240 ggcatgcccg atctgagggc cgctaaactc tgaatgtcac gtgtcacctt ttaatacgtt 300 g 301 <210> 86 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 86 ttatgaaatt atgaggtatg agtatttgga gcagaagatc tttacctcaa ctgaatgcgc 60 ttttaaactc aaatacaagc attaggtagg ctttcctatc aatgctggaa gactttggca 120 ataagagttc tgttaggtag tttgaatata catatcatac ttggttatgg aatgtaaaag 180 gttagctgta agcaattgtc aacacacatc ttaatgatat ttttttctcg tgcctatact 240 ttttcttttg atataaaggt aactatatat tcacaaaaac aaattcatca ttcaaacaat 300 g 301 <210> 87 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 87 aactaaaaca gaaaaagaga aaatctggga tattgaggag ttgaagtttg aagagaatgc 60 ctatgaatta ttgtcattgt acattatctt caggttttca tgttacttcc atgtgatcag 120 tgcattattg cattcatgta gggtcctttg ttaaccaata atttggtaac tattcatgta 180 ctaatttcta cataagttac tcaaaaatac aaaattaacg caaagttcct tttttgcaat 240 ttagaaatcc gtataactaa tacctgtaca agttatgaga aaatttgtgt attatctgat 300 g 301 <210> 88 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 88 acagagcttc tatccaagaa gacggcaatc cacatcacaa agagtttggc ttgattcaac 60 caatgagcgg aaggagattg tccaaagctg tacacactcc atgatgtgcg tgccaggcat 120 gttttggcat cagaacttgc ttaagtttta tctcctcttt tatcattgtt tgtcttgatc 180 cactccatat atattatgat gtttgtgctc ttggtctggc agtatgtctg ataatttgtt 240 cctttgttga acgatccttg gaaatgcttg tgtttttcat tcccgattca actaatcctt 300 g 301 <210> 89 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 89 cagaaaaatg agcttggaca tctatctatt aatctacttg aaaatcttgg ttttaactta 60 atcataacaa ctacttggta taaaaaaagg aacttattta aatatgcatc aatattatgt 120 attttattac tgtagtggcc agactaactg gtgcacacga ctattccata tggtacctaa 180 tacctccaat tagcacggac accaaataac tggtcaccag tgtaggtaaa tcagaagaaa 240 tcacctagag ctttttgtct tggttgtaac acattttatt gacctctaag tcacacctag 300 a 301 <210> 90 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 90 aatattttac tttttgtact ccttcacttc ctccattatg gcccttggaa tttctccaaa 60 catcttcttc ttcaaatttt gtattgtctt ggcaaatgat tctttcatcc tcttggcatg 120 tggagtatat ggatgttgaa cattctttga gagaatgtcc ttcatccccc tcttgttttt 180 gaattcattg acagtttgac tcaaggtttg aagattcatg atagaatctt tatcataatg 240 tttcttgcac ttctcacaga gaaagccaca acatcttcta caagaggtag ccccttcatg 300 t 301 <210> 91 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 91 aaatgggatt ttgcccaaat tttggatttt cagccgcttt gtgggtgcta tgtttggtca 60 aaaaggttcc aatgcactct tctgtgttga gtactattgt tgtgatcgtc ctaatcctac 120 tctccaggtt ctcaattcac cttatttcaa cttcaaatca gttactgctt catcacattc 180 tatgctgtac aataatggtg aaaatgatga tgtcaagatt tcacaacttt tttgcttaat 240 ttgatagtgt agcaactttg aatattttgg ggagttagct caacttgagc aatataataa 300 t 301 <210> 92 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 92 tttatacccg tttgacaata agggaaatat ttttgtaata accagctcga agttgaatta 60 ggatacgaaa attccttaac atttcctttg cattttaggc cagttacaat ggcaaattcc 120 ttcattccaa acgccaatac attcccatta gtatgacgaa catgcagtac atctttattt 180 tcatgtccca cctccaacaa cagcaaacac ttggtaatct aaccctggaa attacacttt 240 ggaatttcca aatatggtcc aaaaattgtt ttcttaaaca gtgcaactcc ctcgtctttt 300 a 301 <210> 93 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 93 ttcagtattc agaattcata aactcgaaat tctgatcgat cattgaattg ttaggtgttg 60 ataggatgtg atggtgtgaa ttcaatggtg gccaagtgga tgggccttcc aaaaccaatt 120 gatgcaaatc gttcagcaat tagaggatat cttgagtatc caaaagccca tggatttgag 180 cccaaatttt gtgcttattt tggtggtgga gttcgtattg gttttcttcc ttgtgatcac 240 aaaagtttgt actggttttg cacttttact ccatctgatg tagattgtaa gtcttctttt 300 t 301 <210> 94 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 94 cggcgggtgt tgatgtaatg acatttggcc agtacatgag accatcaaag cgtcacatgc 60 ctgttactga atacataacc cccgaagcct ttgagaatta tcaagttttc ggcacacaaa 120 tggtttgttt actcctcctt tctttttatt atttaaagtc tttctgcata tcatgttcct 180 gagtttgtat tgggtaatat agtatattgc ttgtgatgga gatactatat cacatctaaa 240 gaaaatatat atctcaatgt ctaatctgtt ttaaggtttg gatattgtaa gtttgctact 300 a 301 <210> 95 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 95 aatgcttcta agagggtaaa aatttatgtg tacagaatat tcccgtccaa tcaacttatc 60 ataattaagc aattcaatga gagaggcata cgaaaaaaca cattacaaac aagtattgat 120 taacttgacg caaacattcg gcaggaataa attttaaccc tgggaacaag caatactacg 180 acaagaagaa gcattaaaaa cgattataca aagtaatcac gtacagttcg tgccaaatcc 240 aattgtttcg ccttctaaat cataaagcac attgaatcca cgctgttgaa ccataccaat 300 a 301 <210> 96 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 96 aatcattata actccatttt gagcttcaaa actacaaaca atatcaagaa aaacttcctc 60 gccattgtta aaggaggaca aaagtctcac atcgatggat aatgaaatgg gtagacttct 120 tataacgctt gggcaatact cctctttttt agctaacttt tgggacgtaa gttaggccta 180 acacctaatt ttacgtgata acatagttat gaggtaggtg ttaaagaatg acaaaaggac 240 aatagtccta cgtcaatggt taatgagata gggcaaaact tggcaattct cctcctcaaa 300 t 301 <210> 97 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 97 aagtttgagc tcatcatcca aagcaaaaag cttaaactca aatctatgac catgatttgg 60 caacttaggt ccacgccatc ctgggacttt ttcatcatta attccttctt taacatgacc 120 atattcttca ccattaccag aaaatccttc tggcaaacct tttaaacttg gtggaatatt 180 agtcacaacc caaataaccc atggtacaat aggtgcatca atatcttgta ctaatagtga 240 caaacttttt gtgtcttgtg gtaaattgta ccattctaat ggtggtgaca tttttctttg 300 t 301 <210> 98 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 98 aattccgtcc ttaaactttc tagcaataaa ctttgaatta tacaacttat ttctgtttaa 60 ttgaatacac ttgtaaatag gatgatagtt ctttacaata aaatcacctg agtccctgtc 120 aatcgaagca tataaccact tacacttaac ttttttgcac ttcaccctca ctctatgttt 180 ttcattaggt cttagcttta actgtcttct atactctaca gcataatctg ccactgcttt 240 cctgaactga tctgcccctt caaagaccat tccaagctca aaaattgaca cttcactatc 300 t 301 <210> 99 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 99 ggtcaagttc gtgaattttt ctcaagtcag gtcaggttca atttttgatt gggttttggg 60 tcttggattg agctcgggtg ttggtcgagt ttgggttgtg agtcaggtgt caaggtcagg 120 accgagtctc agatcgagtg tcggatcttt ttccagggga tgaaacctag tcgggtctat 180 atatatgcaa agaaggaaca taggtctgat gatgtgacaa tatttatatt agaacggtca 240 tttgtctatt ttctcatatt ttacaagcat tttaaaaatt ttaaaacatg ttattgtttt 300 t 301 <210> 100 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 100 ctgagtacat ttcttggtct ctatcacctc gggtgctcta tatcctgcac tccttgatgg 60 gatagtagga aaacccatta gaggtgtaag accaacatct gagatgcatc cactattatc 120 ttgggtgaga agcacattcg atgacttgat gttgccatga attaatttcc caccagaaac 180 agcatggata tgggcgatac cacttgcagc tccatgggca atcctcaatc tagattccca 240 atccagtgtt cttcccaagt ccctgttgcc tataataaat aaaacaagga gcaatgaaag 300 a 301 <210> 101 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 101 tgtaaggcat gatgttgtct ttcctatcag gttttttggt catgttgaag tcctcccttc 60 tggctcgtaa gtactaaaca aactatatat gtggtaaatt atttaaagaa acacacttct 120 ttttaaaaaa gtatatttaa agaaacaaac atgtcaaatt gaattttttg aagccacaat 180 gctctcaaat tcaaactcaa ttgttcataa aatttgtcgt aggcgaaaat gggttggtgg 240 agaggtcgta caggctcttg catatgatgt gcccattcca ggatacaaaa ctaaaaacac 300 c 301 <210> 102 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 102 agactcaaca aatatgaggt tcctttaact ttggcattta ccagaactgt ctcaatcctg 60 aatcttcctg actggtcact gatgttctaa aatataccac cttttgtcat ttcgaaatga 120 attttccaga aaaaaaaaag gaaaaatatt cacaatttag tatgcatatc attaaggagg 180 aacatacctc ctcagtgttg aaaatgtttt tttctaaaaa gacaggaaaa taatgtgata 240 tttcagcatt gatccatccg attctaaccc ctttggctgc tttgtagagc taaaaacctc 300 a 301 <210> 103 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 103 tgacaaaatt gaatccatca cacaaggtct tccaaaaaca cgtgagatga gatttgatac 60 tttttcatcc tgaaaaaata aaattttaag ttaatttact taaataatag gtacgtatct 120 aagtaaacat atttgagaag tgtataattt tctattagtt tatataattt caattctttc 180 aataaattaa ttgattgtga gttcaagatt atctctcttg tcaagggagt gcaactcata 240 tgcacggggt ggaaagaaat ccaagatata atttaacaca tttaacacta gtgtaaatct 300 c 301 <210> 104 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 104 cgtagataat ataaaatcac atcaacatgc tgacaaattt aaacacaaat gtataaatac 60 tataactact aatcaatcat tgtttgtaat tataaatgct aaacaactct taccgaattg 120 ttcaataact gtcctttgaa aaacagatta aagaaccaat tcttattgtc cactatgaga 180 acgccaaggt taatggccac agaaatttca gctagattct tcttgtaatg gtcttgatca 240 tgcttcctaa aaacagaata aaaatataaa cagtatacca ttcattaaaa agataaagta 300 a 301 <210> 105 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 105 agagaaaatt atatacctct atatgcatta ctaggctggg aagtagtaca agttatagtt 60 ttatccatac tacttgaagg aaattgcagc atcttattct gccctggtca ccaaagaggg 120 agagatgaaa ataatcatgt atattctttt cttgaacttg agaaaaatga tgataatcac 180 gtatatacta tccaacaata tggtaaatcc ttcttttgca ttaatgttta acaattcaga 240 cgaattaaaa gtacaaccaa aattttagct aagaaagctg cctatgataa tactacatca 300 a 301 <210> 106 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 106 ccggaaaata atgtggtggt ggaaaattat agttagatga tggtggtggc agtgacggcg 60 gagagaagat aggctgacat gggttgtcac aggaagaaca cattgtgcag gatattcggt 120 ctgccgttgt cgacgtggcg tgagttgatt ggacattgcg aagcaaaata agggtactga 180 agactagaaa ttgaaaatgc aggggagcca tttgtatgaa aacagaggag atatgaaaca 240 ggggaaaatc aggttatgca caaaaagaag agttgtttat ggtagtatac acaatagaag 300 a 301 <210> 107 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 107 atttgaatat ttagttacca taatcttatt ctattcatgt atttatatca ttatacactt 60 taagtaatat acaagttatt cttcatacca tatgaattgc acgatatccg agtcaggtca 120 atccccattt gggctcctgg tctacaatgc ggttgggttt ggaggtgaag ggggtgttag 180 agtggataaa gtccgacatt ggttggggaa tggcctgatg gtttgtttat atggccttga 240 caatctttac aatttactta acaaacctta tgaattaact tcttcttttt ttgaaattgg 300 t 301 <210> 108 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 108 gaattacaag ttctagttat agattcctct atgggaaaga atagaataag taaagaaaag 60 accaggacat acccgacgac aatgtagttg agactttcca tttactcttc aagaatgtgc 120 gagtagatgg agatagtgaa gctggaagca acttgaagcg ctcgtagatg cataaaccag 180 cagcacactg caaagccatt acataattag gagactaatt gcagtgatac caaaatatct 240 aacaattaaa gttagagaag tcacatttca ctgtattcaa tatcacattg aaatgattgg 300 a 301 <210> 109 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 109 ttaatgactg acctagttat atttaggtat gctgcacttt ctagttattg gagaactcaa 60 gtactctctt tcagtaccta tagttggcta tttgtctaaa aggagtcaag aactaatatt 120 tcttcttttc tcttcaaccc gacacgtcac agtacgcaac aactaatact tatatagttg 180 gctatttttc tgaaaggagt caggaactac tttcatcttc ttttctcttc aactctacat 240 gtcacataat tgatacttaa ctagttgggt atttgtctaa aaggagtcga caactaattt 300 g 301 <210> 110 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 110 ttggtaaagg attgtattct agtgcctata aattgggtct caatgtaata atgtagaaac 60 aataacaatt caataatatt cttttcctac atttctcaca acttcagaat tttctgatca 120 attaaggtaa ctataaacat ggtggtcgtc atggtttgag ggatgtggat gacacttcta 180 ttggggaagg aaaacccgtg agaaggggag gcacttctgc ttacaattct cttgaggtgt 240 gttttctttt taaattctta tttgtacttt gatctttgat tttcccttgt acatttactg 300 a 301 <210> 111 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 111 ttgtcgctat cactttcaca tatttcctcc atctctatat ccctccacat cgattcaatg 60 aaacctgtat atcacaagtt aactacatca gattaagttc taacaccaat atttatttgt 120 ttttttggtt ttcaattgta tgctcgaagt tcacattaga gctttcatta aatttgaatc 180 ccattctaca aagcaaattt gagtgtgctc caaaaaagga ttttcttcat attcagggct 240 caaacacaaa agctttggtt aagagtgaag taaacagttt ctactcaatc ttctttgctt 300 t 301 <210> 112 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 112 acttcatgtc aaatcaaaat cataaaaaaa ccaaattgaa agaaaaagag tatctatttt 60 tttatataaa tgaattaacc ttttttaact ttaaatacac aggagtattt atcaataata 120 ttccaattca ttgagtacac tttctcttag aatcatggaa ctctgacgga taatcgtaga 180 tgtacacaaa aaaacattaa agagtttttc gaataaaatt atttcataat gagactcaac 240 tactatccac agtaacagaa accagagctc accttcgaaa caagatgtca ctatgatcca 300 a 301 <210> 113 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 113 atgtaggacc attatagctt atcgaaagct ttttcaaggt caatcttatc atgttagcat 60 ttttccctct tatttttttg aggtgagaga taaattcctg gaaaataatg acattgttag 120 aggttcttct attggagaga aagctagcct aattggggcc aatgatatac ggaagcatgt 180 gcttaattct gttgagaatg atcttggtga tcaacttagg tggtatttca aaacccgatt 240 ggtttgaagt tcttgatgga ggtggctggc atatgggctt ttagtgataa ggcagagcat 300 a 301 <210> 114 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 114 caataataac aaaaataaca acaacaaaat caataacgac aataacaaca acgacaacaa 60 cgatgaaagc aacaataaca acaatgataa aaatgacgat atcaataaat acaacaaaaa 120 ctacaacaac aacgacaacg atgacaacaa cgacaacgac atcatcgaca atgacgacaa 180 cgataacaac gacaatgata acaataataa cgacaacaac aacaatgaca ataacaatga 240 cgacaacgac aatgatgaca ataacaacaa caacaaagac aacaaccata accacaacaa 300 c 301 <210> 115 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 115 aatccacatt cgccagaaaa ttgcacagat ataaaatcaa aatgcataac cagcttcaga 60 agtgcttaag tagacactta aacttgtaaa aaaatcgaac aagtaggcac aaacatccta 120 cgtggcataa tacacgtaag acatcatata ggaccaaact tgccacttag gacgaatgag 180 tatacttgtt tagttaaata caagcttaag tgcacactca aagttagaga catagatgtc 240 agttgacacc aaattaaatg acacattcat gtattatgcc taatcaaaac tacatttact 300 a 301 <210> 116 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 116 gctagtagat gcttgttgac cagacaacca cataaatgac acttcaattt attttgtttt 60 ggtgaccgtg gggtccaggc tagctttcgc acacctcggc tgattacacg ggataccttt 120 cacctcctaa cagcaacacg tctcagaaca gataggaaga aatcacctaa tgtttttgtc 180 cctgttggga attgaacctt agacctcatg gtgctcaatc cacatcattg accactaggc 240 tacacctttg ggagcataaa tgacacttca aattgacttg tctatgaaca agaacagagc 300 t 301 <210> 117 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 117 ccagaagcac aatggtcatt agtgttaccg caccacccga atttgctgca acattgtccc 60 gacgcacaaa ctttgcctcc gccctgtgaa ccacaattct gtgctgaggc agcactcagc 120 aaaaccagag aaaatactaa agaaaaagta gtgaatttag aagttttcct cattttgaat 180 gtgatggcaa acatgttgca aactgaaata tatttatagg caactgcttg tgaggaatca 240 gtgatttttg ttacaaaatt gatagtccgt cctgtcttat attgaatgac agctttactt 300 t 301 <210> 118 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 118 agagttgagt tcttctccat aggaccttct tcaattgtag gtcgaacaat agcctttcga 60 gtcttgtttt gcaaatcaat atcgcggttg aggcgtagta tttttcattt catgatgtat 120 tacttgtaca agatcaagaa ttgtctgtca cactatttaa cacctttgat caaatggttt 180 cacccgcgta atccacaagg gatactagtg ttagtaacac ttctagcctt cttgttgagg 240 cgatatacgt atgttaaaat cagggctgat atggtttata agaggaaatt ttggaggaat 300 a 301 <210> 119 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 119 ttaaataata gaaaacacag gctcaatatg acataaaaag tgaagagctc aagcaactaa 60 atttgctacc gacaacaatc aaaactgtga ccagctaagt ctaatacctt agataagatc 120 ctcttagttc attttctagc agcaaaggaa tgcaatgaat tactagttac actcctcatc 180 agttcaaaag gagaatgaaa aagagacatg tcaaaccaat gatgcacagg gatgagggat 240 cccagtcgca gtaaatgcat gcctagttgg caactaaaaa atggagaagc cacactatga 300 t 301 <210> 120 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 120 gcaatgaggc ttgccttaga aagtcatatt aaagtagttg gttgtaattt gtcagccctt 60 atgctatgaa agagaagaga agcgacttga cttgccagtg gcctcttaaa aatagcattt 120 tcttgagaga attccttcag aacttctgga attcgctttg tgtgtaatta aatttccgaa 180 ggaaatctat aactttaatt atttaaattt gggtaacaaa tatcaaataa atgctcatgt 240 tcacttagat gaattggaaa attgcatttt aacttgaatc caaaagattt ggctccccta 300 t 301 <210> 121 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 121 gataactcaa tcatttctaa gactcgtgga tgaatgggat atttctttac gaggtcccaa 60 aattttccat cacagctcct cgtatttaaa atcatattaa cacttccatt ccatatatcc 120 cgtgacctat gggtaagttg ttgaattagt accgatggtt caagtggatc ggcaaccaat 180 cgatatgcac tataaccagt catatacgta tctattaaac tgaaaaatcc atatgaaagt 240 aaattaaata taattgttag tccaaaatat aacaaccaaa tcaaagtaat gtactgtcac 300 c 301 <210> 122 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 122 ttaattttgg gaagttgttg taataatcca catttgttcg attggttcgg tttgttgtga 60 tgctttctca ttacttgaag gtgttccaag actgattagt ttgcttccca tcctttcctt 120 gaccctgctc tgagctatgc ttgccttcct ccgcactccc tgtctgctca attccttgcc 180 ggagtgctcc aattttttgt ttgagtgttc ttgcctttgc atttgtaact tcccattctc 240 tttacctgtc tcatcttggt tggttagggt ctgtggatta catgcattca agttgggagt 300 t 301 <210> 123 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 123 ctggtgtgtt gtttgttcta gcgataggat ccaagcacga ttctgattct caattttttt 60 tctattctgg aatgaatata atctaaggaa atacctgcct atactgaaga ggggcaggct 120 caaggcaatg aatggtggta tcagtcgggg caaacacaat actcattccc gcaacccttg 180 ttgcagttgt tagagtcaaa tgtaatggca cgagaagttg tcttaatcaa aaaaccttcc 240 gaatggaata agctactata actttctttg tcgtcaaagg aaaatggaat cataccttag 300 t 301 <210> 124 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 124 cctctaatga aaggactgga tgagactgca accaaattta tacatgttag ctgcaacaaa 60 catacctggt ccgttcaaat ttgtttgtct gattttaact tgacatgaag tttaagaagg 120 tcaagaaaat tttaaatctt gtaattaaac taaagaaatg tataatatac aaaaatatgc 180 tttaatgtgg tcttaaacat gtcatgtgaa aagttagaat gaaagagata tcaaaaagga 240 aaagagattt taaacggaat taaaagaaaa gtaagataca taaattgaaa cagacgaagc 300 a 301 <210> 125 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 125 gattgagtat gaaaaggctg caactggagc aacaggtcca caaaaactgt catcgagcct 60 catttgtatg gactatcaac tctaaacttc tccaaaatta gattagattc taaaatctga 120 ttcaagaaaa tgctcttacc ggttatgctc gcctctgcag ctattaccct gaagtgtgtc 180 gcagcatcaa ttggtgaaaa agtcccattg actaaaaagc tgccggcaac aacaacagta 240 tgatcttttc atttaatagc caagcatttg attcctctat ggtttaaggt ttttactcat 300 c 301 <210> 126 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 126 ccctgataat tgcgtcgttg tgcttgggct gtgatgtcta tcagccacat ggaaagttca 60 atgtgctcat cggcgcttga ccttgagatg tggatcatcc aaggcacatt agcatggcgt 120 actccacctg ttagaattag tgtttgaaac caaaatcctc ctcaggagga tagatgtggc 180 aattcggttg agatccaatg tagggaggga ggaccatcac ggctcctctc ttctcgagaa 240 ttcatacatc ccttatcagc atatggacac ttatctctcg agcataggtt taggttcaac 300 c 301 <210> 127 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 127 gaagcttctt tttatcgcta taaggcataa gtagtaaggc aagtagtaga cctataaaga 60 gaaaaggaag cgctatccct cctacttatg attcaacatt tagctcgatc tattctagat 120 gtatttttta catcaatggc atcagctgga tcttgcaccg aattgcttac ccgcggaact 180 catttaaccc gaccctacat aagtactttc acctctagga aaaacttctg ggatgagccc 240 ttctactcgt aattttttca ttttgatagt agagggaaaa ttatcgatcc cagaccaggc 300 t 301 <210> 128 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 128 tataaatagg gtctttgtgt aataatgtga aacaaacaat tcataacatc ctcctatatt 60 tctcacatgg tatcagagtc tctacaatat tggtagagaa tcaaagagct ctcattgtca 120 ccgggtggct attacccata tatgttgccc gtctagcgaa caattatgtt aacaaaagta 180 ggtcgatgat atatgcatca aacatatatt caggggaaga aaacttagtc aaacagctca 240 cccgcacatc aatatttggt gacttcctag ggttgttatg tgtcaacccc atctttacgg 300 t 301 <210> 129 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 129 cttaatgaac ttgctgagga tctgaagcag gatgttgaaa tggaagataa gtcatcagat 60 attttgcata atcctatttc agaggaccta agtttgttga ggatgaagct tgaaggagcg 120 caggaaaagc ttctaaaatc tgccaatacc ataagtatgt ttggttcatt agagagagca 180 attgttgagg ttgatgaact tgcagaagaa attgaaggac tcgaaaagag cattgaagtg 240 aaaaaacaag gatacacatc cttcaagctt cagtcgtcac aaatgcttga aaagaaagtt 300 t 301 <210> 130 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 130 attatctgcc cttagttgag gttgttacca agttgtgatc tgcctttgtc ttcatgatac 60 gccaataacc tgtgatatac ccacttcttg agctttttga aattcacttt ctattgcaag 120 atcaccaatg tttctttttg actatacaca gttcattctg gctatataac ttgatatcat 180 acgcaatttt gttgattttg ttatgtagtt gatgaaaaag ttactggcgg acctttttct 240 ttcttcgtcc tatcttttat ttccatccct tactgttatt atgaaggtgg gcagctcact 300 a 301 <210> 131 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 131 aaggaccaaa tcataacaag agaaattgtc caaaaaattc taaactaagt ctacaccaac 60 acccagttaa gaggtacatt catattgatt tgtttttata aattatttta gattacttat 120 taaatttaat gtcttgttgt gttttttttt tcactcatat tagtccacca ctggaaaaaa 180 gaggggtaga ggtcattatg aaagagcaag caccagtaag actggtacaa gaagaggtgc 240 aggaagtggt tacaggaaga ggcctaaagt tgttggacaa ggtgtatttg ttgctgatac 300 t 301 <210> 132 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 132 ccggatgcat cagcttgtaa tacagtcaca ttcgataact caccagtcgc tgacacaata 60 caaacagccc ttggactact gtgaataaat gacaatattt ttgatgacac atcctgaaaa 120 tcagataata aggatttgta agatattgtc tgtgactccc aaccaagtta acttctaccg 180 gattttatga attattataa caaaactgat atggaatagt tgcaattcaa taagaatgtt 240 tgctggctat tcccaagttt caaataaaac taaatggttt tcaagtcaaa ggaaatagaa 300 a 301 <210> 133 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 133 caaacactcc tttccaacag actgcaatcc atctccagat gtctgataaa gagggaagtt 60 gtgtttctcc ctcatcaaaa gagcccacaa tgcacctctt gaaaagtcca gtgtctcttg 120 cccttggtgc ctctgcaagt tgaaggtttc tttcactgta cgagatctta gtctcacctg 180 aagtgtttga tatccagctt tctccagctg ctgtggcata agaaaggtcc ttcttaaaat 240 gtttgggttg tactgcattc ttcagttggt gagagcattg aatgaacctc tctatcttag 300 a 301 <210> 134 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 134 atcggagtgt cacgtttcga cacatagtat taggggatcg gagtgtcacg ttccgacaca 60 tataggggat cggagttgtt tctatctact attatctaaa aataatcaag ttattaatga 120 ctggacgaaa ccaagaaata acccctcacg agcgaaatta tagaagatcc cctttaagat 180 caaacaattc catcgaattg agtatgattg tatgtgtgat agcatctact ataccaggaa 240 tatcaatgaa cccaattatt gcaattgctc aggatagcct tttttagctt ctagggtcta 300 t 301 <210> 135 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 135 tgcaatcttt cccctactta ttttcttttt gtagcaatcc agatatcacg ctagctgaga 60 gttgggcaaa tggttgggat atatctttca ggatacgttt gaatgattgg ggaaatagat 120 agggtggcac aattgttaca tgcaatcaac aagttcaatg gttttgctgc aaggacagac 180 ggtctcgcgg gttcactctg aggaaggaag gttcacaatt aacaagctct ataagaagga 240 actaggaact caataaagcc tcaggttggc tagattgaaa tatgtatgga caagccaagc 300 a 301 <210> 136 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 136 cttttatggt aatagcaaca gccgttacgg caatatttgt gcaaatcatg gtgaaatggc 60 tttatgatcc atctaggaaa tatgctggat atcaaagaag aaacttaatg aattccaaca 120 acaaattacc catactagtt tgcatccaca gccttgataa cactgctgct atacttagac 180 tgctggagaa atctaatccc actagggatt tcccaatagt gtccaatgtt ctccacctta 240 tagagctacg cggtcgagca tcttctgttt tcatctctca ccaagttcag acaaaggcta 300 t 301 <210> 137 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 137 aaactgcagt cgagttgttg aagctcattc accgatggca attgtgattc ccaatagagt 60 ctccagcttg agcatagaat tacgtgatgg tgacatctct tggcctgact ttttagacag 120 agtgaaagat tccaagtaag aactcctgcc tcagagattc ttataatttt tttggtacat 180 gtttaatcta ggaaaaggac aatatagtgc ttgttctttt ttttgtcata cttaattgtt 240 gccaatgaat tgggtcagag tttggaatgt tatatcaact tcttctttta aacattaaga 300 a 301 <210> 138 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 138 ctttatagga ggttttgcaa tttttttgcg aacattagtc ggataggatt gaaccctctt 60 gtgatccatt atgaattcga ttcacgatgg cttggagcct acttgagatt ctgtttatat 120 ttcgcacatc accaaaagta ctcgtcatta caacaaaaag accataaatg gggaaaagaa 180 actttaactt agtcatttta ctgcactatg tgcttcactt catctatcaa agatttatgt 240 gtttcatccc ccttaaagta cattcaacaa ccttattatt agaacttttc taacagaaag 300 a 301 <210> 139 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 139 cctttggagt ggtctgcacg tcaaaagata gctgttggag ctgcacgagg tttgcgatat 60 cttcatgaag agtgcagagt gggctgcatt gtccatcggg acatgagacc aaacaacatt 120 ctcatcactc atgactttga accactagta agggcaacct cttactaata agaaagttat 180 tcaagataaa cgttaacttc taaccaaggc tccttcagag cataaaaaga gtctgcacca 240 gtgtctcgta tttccctgtg tatctattct tgatagctaa tgacttgtca aaattctctt 300 a 301 <210> 140 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 140 aacaacaaca acaacaagaa catcgacagc aacaacaaca acaacaataa caacagcaac 60 aacaataata acagaaacaa taataaaaat aaacaataac aataacaaca acgtcaataa 120 caacgacaag aagaagaaga agaagaagaa gaacaacaac aacaacctca aataaccaca 180 acgatgagta agaacaacaa cataaacaac aataacaaat aatagcaaca acaacaacaa 240 caacaaaaag gacatacaca acaataacaa caaaaagatc aaaaacaaca acaacaccaa 300 c 301 <210> 141 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 141 tttaccttct tttctaattt tatgcctact tttttttttc cttcactcta tttaatattt 60 tttactttac ataagtttaa aaaagaatta aaaattggca aataagcgtg caaacacata 120 aatatattct ttaggcatgt atgaacatat caatgagtta aacttctttt aaaaaatcta 180 aaaatcgtta taagatataa gtaatgagca attgaatatt tgaacgggaa cctagaagct 240 tatttggaat aatttaattg tttatacaat tgtcgtttct aattgtacgc aaccaattga 300 a 301 <210> 142 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 142 aaaataggat agaggttatt gttccctata tgcaaccagg gttatcgtgc attgtgaaga 60 cataacaaat taaatagggc ttttacctat atgcaactag gggttatcct tccctatgaa 120 gacacgaaaa ttaggatagg agttaatgtg tcctatatgc aactaggggt tattatgtcc 180 cgtgaaggca tgaaaataca attggggggg ggggggggtt attgtgccct gtgaaggcat 240 gaaagataaa atagggaata tagtgcccta tatgaaacca ggagttattg taccttgtga 300 a 301 <210> 143 <211> 301 <212> DNA <213> Solanum lycopersicum <400> 143 ataggagcac tcaaagaatt taattttgaa gcaaataaga ccagtctatt gactcgcaca 60 acttgtcatt gtcattgtta gtaaaattaa tactactatt actcataaag ccaggtgtga 120 tgcagataaa gaacagattc cacggttgca cgggatatta ctagctttca aggcagtttc 180 tggcttgaaa gtgaatcttc ttaagaacaa catcacaaca taattagttt ttatgcaccc 240 ccaagattgg tgggcagctt ctattagctt ctccttatat ttgaaggctg acagacaggt 300 c 301 <210> 144 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 144 actctgcctc tgctggatcg 20 <210> 145 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 145 ggatcttcct tatgccggat c 21 <210> 146 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 146 tgcatgacta atctgttaag gaagaaggct t 31 <210> 147 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 147 ctcccttcta gtgtaatatc ag 22 <210> 148 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 148 ccctgaatct gcaactttct g 21 <210> 149 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 149 ttgcaaacgg cttcttctgt ctctgagaag c 31 <210> 150 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 150 ccccttaacc acttcactag a 21 <210> 151 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 151 aagggcggag ctgaaggga 19 <210> 152 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 152 agccccaaaa aatctctttt tgccttattt g 31 <210> 153 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 153 atgcagccaa tctaactacg aa 22 <210> 154 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 154 gatggtttat tatcctgttc tgtc 24 <210> 155 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 155 tggtgttgga caagagttag tgaacaaccc t 31 <210> 156 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 156 aaagttgttg gtcctgttgc tg 22 <210> 157 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 157 ctgcagcaca ttaacagaat ac 22 <210> 158 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 158 gtgtggcaca actgcaatgg tatgactgtg g 31 <210> 159 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 159 gagaatctaa agagccactg aa 22 <210> 160 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 160 aaattgcaca gtaaacttga gga 23 <210> 161 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 161 attattgaat tttatcccaa tactgatagc 30 <210> 162 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 162 ctcaaatttt cgcagtgcct ca 22 <210> 163 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 163 aggaaccaag atctcctgct g 21 <210> 164 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 164 tacattgtgt atgtcattgt tgttattcta t 31 <210> 165 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 165 aggaccttga attgcacctt gt 22 <210> 166 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 166 ggatctaaat ggcagtttgg g 21 <210> 167 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 167 atatagaact aggtcttccg gagatgactg t 31 <210> 168 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 168 tcatggcttc tggtaagtcc ta 22 <210> 169 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 169 catccacaca acaacctgtc ata 23 <210> 170 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 170 tgattttatg gggtttcgtt agtatgtttc t 31 <210> 171 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 171 cttttgtaca tcatcctgca tg 22 <210> 172 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 172 gttagccaca atctaacatt aag 23 <210> 173 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 173 acaaggacat gagttgaaaa agtatcttca g 31 <210> 174 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 174 cacctctgca tattgctttg g 21 <210> 175 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 175 ggtggtaact gagaagggat t 21 <210> 176 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 176 gtaaatgctg ctggcataga agatacagct a 31 <210> 177 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 177 cttttgtaca tcatcctgca tg 22 <210> 178 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 178 gttagccaca atctaacatt aag 23 <210> 179 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 179 aaacggaata taaaagcctg ttgctttttc c 31 <210> 180 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 180 gtagtactaa taatgaagag ctc 23 <210> 181 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 181 cagcagcaga agtagatatt gaa 23 <210> 182 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 182 tgtgcgccat ttccagcatt tccattgctg t 31 <210> 183 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 183 atcccttgca ccttgagtat tt 22 <210> 184 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 184 atctgcatcc ctaatgtctt ca 22 <210> 185 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 185 gtacaaataa gcattacgtt tgatcgtgtg t 31 <210> 186 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 186 agactgttgc tgcagctggg 20 <210> 187 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 187 gtcactgaaa atatgtggtc tta 23 <210> 188 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 188 gagcttgata cctataaaaa tgaagttgta t 31 <210> 189 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 189 tgattcccag tggcaaatgt ac 22 <210> 190 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 190 ttcgtatgag tttcccattt gc 22 <210> 191 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 191 accaacgatt gaagctaccg tctgttaaca g 31 <210> 192 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 192 taggtttagc agcgattttg tg 22 <210> 193 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 193 caaaccacca cctgaaaccc 20 <210> 194 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 194 tggttgtttg caaaaactca ctcaaaaggg a 31 <210> 195 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 195 gtggtcagtc ttacacagac 20 <210> 196 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 196 gaagcagcaa cagacaaagt g 21 <210> 197 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> probe <400> 197 agaacacaca atctgcctct acatttcagt t 31

Claims (6)

  1. 서열번호 1 내지 143의 염기서열로 이루어진 폴리뉴클레오티드에 있어서, 각각의 염기서열 중 서열번호의 151번째 염기에 위치한 SNP(single nucleotide polymorphism)를 포함하는 10개 이상의 연속 염기로 구성되는 폴리뉴클레오티드 또는 이의 상보적 폴리뉴클레오티드를 포함하는, 마커이용여교잡(MAB, marker assisted backcross) 육종을 위한 토마토의 유전적 차이 진단용 SNP 조성물.
  2. 제1항에 기재된 SNP(single nucleotide polymorphism)의 폴리뉴클레오티드 또는 이의 cDNA를 포함하는 마커이용여교잡(MAB, marker assisted backcross) 육종을 위한 토마토의 유전적 차이 진단용 마이크로어레이.
  3. 제2항에 있어서, 상기 폴리뉴클레오티드는 아미노-실란, 폴리-L-라이신 또는 알데히드의 활성기가 코팅된 기판에 고정되는 것을 특징으로 하는 마커이용여교잡 육종을 위한 토마토의 유전적 차이 진단용 마이크로어레이.
  4. 제3항에 있어서, 상기 기판은 실리콘 웨이퍼, 유리, 석영, 금속 또는 플라스틱인 것을 특징으로 하는 마커이용여교잡 육종을 위한 토마토의 유전적 차이 진단용 마이크로어레이.
  5. 제2항의 마이크로어레이를 포함하는 마커이용여교잡 육종을 위한 토마토의 유전적 차이 진단용 키트.
  6. 토마토 시료에서 게놈 DNA를 분리하는 단계; 및
    제1항에 기재된 서열번호 1 내지 143의 염기서열로 이루어진 폴리뉴클레오티드의 각 SNP(single nucleotide polymorphism) 위치 염기인 다형성 부위의 유전자형을 결정하는 단계를 포함하는 마커이용여교잡 육종을 위한 토마토의 유전적 차이 진단 방법.
KR1020170062466A 2017-05-19 2017-05-19 토마토의 여교배 육종을 위한 단일염기다형성 마커 세트 및 이의 용도 KR101915074B1 (ko)

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* Cited by examiner, † Cited by third party
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KR102264689B1 (ko) * 2020-06-18 2021-06-14 세종대학교산학협력단 토마토 품종 식별용 마커 조성물 및 이를 이용한 식별 방법

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101539737B1 (ko) * 2014-04-18 2015-07-28 주식회사 씨더스 유전체 정보와 분자마커를 이용한 여교잡 선발의 효율성 증진 기술

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101539737B1 (ko) * 2014-04-18 2015-07-28 주식회사 씨더스 유전체 정보와 분자마커를 이용한 여교잡 선발의 효율성 증진 기술

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102264689B1 (ko) * 2020-06-18 2021-06-14 세종대학교산학협력단 토마토 품종 식별용 마커 조성물 및 이를 이용한 식별 방법

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